{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001330","ANALYSIS_ID":"AN002216","VERSION":"1","CREATED_ON":"March 22, 2020, 8:59 pm"},

"PROJECT":{"PROJECT_TITLE":"Rice panicle blast resistence","PROJECT_SUMMARY":"Metabolomics studies of OsGF14b-mediated innate immunity against panicle blast in rice","INSTITUTE":"Guangdong Academy of Agricultural Sciences","DEPARTMENT":"Agro-biological Gene Research Center","LAST_NAME":"Yan","FIRST_NAME":"Shijuan","ADDRESS":"No. 20 Jinying Road, Tianhe District, Guangzhou City, Guangdong Province, 510640, China.","EMAIL":"shijuan@agrogene.ac.cn","PHONE":"+86-020-38213643"},

"STUDY":{"STUDY_TITLE":"Multi-omics of OsGF14b-mediated innate immunity against panicle blast in rice","STUDY_SUMMARY":"In the present study, we used a multi-omics approach to decipher the molecular mechanisms of OsGF14b in governing panicle resistance to Magnaporthe oryzae.Results revealed OsGF14b mediated panicle blast resistance was involved in the activation of auxin and JA signaling pathways, resulting in reprogramming of the phenylpropanoid and diterpenoid pathway.","INSTITUTE":"Agro-biological Gene Research Center , Guangdong Academy of Agricultural Sciences","LAST_NAME":"Yan","FIRST_NAME":"Shijuan","ADDRESS":"No. 20 Jinying Road, Tianhe District, Guangzhou City, Guangdong Province, 510640, China.","EMAIL":"shijuan@agrogene.ac.cn","PHONE":"+86-020-38213643"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Oryza sativa Japonica Group","TAXONOMY_ID":"39947"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-0h-1",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-0h-1"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-0h-2",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-0h-2"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-0h-3",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-0h-3"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-0h-4",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-0h-4"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-0h-5",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-0h-5"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-0h-1",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-0h-1"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-0h-2",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-0h-2"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-0h-3",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-0h-3"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-0h-4",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-0h-4"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-0h-5",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-0h-5"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-0h-1",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-0h-1"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-0h-2",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-0h-2"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-0h-3",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-0h-3"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-0h-4",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-0h-4"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-0h-5",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-0h-5"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-0h-1",
"Factors":{"Genotype":"Wild-type","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-0h-1"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-0h-2",
"Factors":{"Genotype":"Wild-type","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-0h-2"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-0h-3",
"Factors":{"Genotype":"Wild-type","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-0h-3"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-0h-4",
"Factors":{"Genotype":"Wild-type","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-0h-4"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-0h-5",
"Factors":{"Genotype":"Wild-type","Treatment":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-0h-5"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-24h-1",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-24h-1"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-24h-2",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-24h-2"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-24h-3",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-24h-3"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-24h-4",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-24h-4"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-2-24h-5",
"Factors":{"Genotype":"OsGF14b-overexpression line 2","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-2-24h-5"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-24h-1",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-24h-1"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-24h-5",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-24h-5"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-24h-2",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-24h-2"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-24h-3",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-24h-3"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-4-24h-4",
"Factors":{"Genotype":"OsGF14b-overexpression line 4","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-4-24h-4"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-24h-1",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-24h-1"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-24h-2",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-24h-2"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-24h-3",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-24h-3"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-24h-4",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-24h-4"}
},
{
"Subject ID":"-",
"Sample ID":"OXGF14b-6-24h-5",
"Factors":{"Genotype":"OsGF14b-overexpression line 6","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"GF14b-6-24h-5"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-24h-1",
"Factors":{"Genotype":"Wild-type","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-24h-1"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-24h-2",
"Factors":{"Genotype":"Wild-type","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-24h-2"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-24h-3",
"Factors":{"Genotype":"Wild-type","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-24h-3"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-24h-4",
"Factors":{"Genotype":"Wild-type","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-24h-4"}
},
{
"Subject ID":"-",
"Sample ID":"Nip-24h-5",
"Factors":{"Genotype":"Wild-type","Treatment":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"Nip-24h-5"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"The panicles at the initial heading stage of the wild-type Nipponbare (Nip) and OsGF14b-overexpressing plants were harvested before (Nip-0h; OXGF14b-2-0h; OXGF14b-4-0h; OXGF14b-6-0h) and after M. oryzae 24-hour inoculation (Nip-24h; OXGF14b-2-24h; OXGF14b-4-24h; OXGF14b-6-24h) respectively. They were immediately frozen in liquid nitrogen, with each biological replicate containing panicle pooled from 10 individual plants.","SAMPLE_TYPE":"Seeds"},

"TREATMENT":{"TREATMENT_SUMMARY":"Wild-type japonica rice (Oryzae sativa cv. Nipponbare) and three OsGF14b gene overexpressing lines, including transgenic line 2 (OXGF14b-2), transgenic line 4 (OXGF14b-4), transgenic line 6 (OXGF14b-6) were used in this study. Rice seeds were surface-sterilized and transferred to 1/2 MS medium and incubated in a growth chamber for germination under light of 200 μmol/m2/s with a 12-h photoperiod at 26℃. Subsequently, rice seedlings were transplanted into soil and kept in a greenhouse. M. oryzae GD08-T13 was used for rice blast inoculation."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"The rice panicle pre-cooled in liquid nitrogen were ground using a Mixer/mill (MM400; Retsch) with steel ball for 30 seconds at 30 HZ. Fifty milligram of rice panicle powder of each sample was extracted with a fixed volume (1 ml) of pre-cooled (−20 °C) extraction solvent (methanol: chloroform: water = 5: 2: 2) was added to homogenized tissues. After adding the extraction solvent, the vials/tubes were thoroughly vortexed for 1 min and then incubated on an orbital shaker (200 rpm) for 10 min at 4 °C followed by a 15 min sonication step. For phase separation, a volume of 500 µl of solvent (methanol: water = 1: 3), was added to each vial/tube and the samples were again thoroughly vortexed for 1 min. After that, the samples are centrifuged at a speed of 14000rpm for 10 min at 4 °C. Two fixed volume of 200 μL of the polar phase (the lower phase) were transferred into pre-labeled 1.5 ml microcentrifuge tube respectively. Then the samples were dried in a SpeedVac concentrator without heating. Two dried 200 μL aliquots of the polar phase in each sample were analyzed using gas chromatography tandem mass spectrometry (GC-MS) and liquid chromatography tandem mass spectrometry (LC-MS) for metabolomics study. The dried 200 µl aliquots of the polar phase for GC-MS analysis were re-suspended in methoxyamine-hydrochloride/pyridine solution for methoxymization of carbonyl groups followed by heating at 37 °C for 2 h. The samples were further derivatized with N-methyl-N-trimethylsilyltrifloracetamide (MSTFA) for 30 min at 37 °C. then one µl of the derivatized sample mixture was injected onto the GC-column and measured. Another dried 200 µl aliquots of the polar phase were re-suspended in 150 µl UPLC-grade methanol: water (1:1, vol/vol) and subjected to LC-MS analysis.","PROCESSING_STORAGE_CONDITIONS":"-80℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"One µl was taken from each sample and injected into GC-MS at 270°C in a split mode (50: 1) with helium carrier gas (> 99.999% purity) flow set to 1 ml/min, and separated by a DB-35MS UI (30 m × 0.25 mm, 0.25 µm) capillary column. The temperature was isothermal for 4 min at 90°C, followed by an 8°C per min ramp up to 205°C, then held for 2 min, and finally ramped up at a rate of 15°C per min to 310°C, held for 2 min.","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Agilent DB35-MS (30×0.25mm, 0.25µm)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 5975","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"The transfer line temperature was set to 300°C, and the ion source temperature was set to 230°C. The mass range analyzed was from m/z 85 to 700. The Agilent MassHunter Qualitative Analysis (version B06.00) software and the Agilent MassHunter Quantitative Analysis (version B.07.01) were jointly used for GC-MS data analyses. NIST library and in-house database established using authentic standards were used together for metabolite identification.","MS_RESULTS_FILE":"ST001330_AN002216_Results.txt UNITS:Relative content Has m/z:No Has RT:Yes RT units:Minutes"}

}