{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001342","ANALYSIS_ID":"AN002236","VERSION":"1","CREATED_ON":"April 6, 2020, 3:13 pm"},

"PROJECT":{"PROJECT_TITLE":"Toll-like receptors induce signal specific reprogramming of the macrophage lipidome","PROJECT_TYPE":"Shotgun lipidomics","PROJECT_SUMMARY":"Macrophages reprogram their lipid metabolism in response to activation signals. However, a systems level understanding of how different pro-inflammatory stimuli reshape the macrophage lipidome is lacking. Herein, we use complementary “shotgun” mass spectrometry approaches to define the changes in lipid composition of macrophages induced by Toll-like receptors (TLRs) and inflammatory cytokines. “Shotgun” lipidomics data revealed that different TLRs and cytokines induce macrophages to acquire distinct lipidomes, indicating specificity in reshaping lipid composition.","INSTITUTE":"University of California, Los Angeles","DEPARTMENT":"MIMG","LABORATORY":"Bensinger lab","LAST_NAME":"Bensinger","FIRST_NAME":"Steven","ADDRESS":"University of California, Los Angeles","EMAIL":"bensingerlab@gmail.com","PHONE":"+1-310-206-1016"},

"STUDY":{"STUDY_TITLE":"Timecourse of C56BL6 BMDM stimulated with different TLRs (part-I)","STUDY_TYPE":"Timecourse","STUDY_SUMMARY":"C57BL6 bone marrow derived macrophages were stimulated with TLR2, TLR3, TLR4, TLR7, and TLR9 ligands for 24 or 48 h.","INSTITUTE":"University of California, Los Angeles","DEPARTMENT":"MIMG","LABORATORY":"Bensinger lab","LAST_NAME":"Bensinger","FIRST_NAME":"Steven","ADDRESS":"University of California, Los Angeles","EMAIL":"bensingerlab@gmail.com","PHONE":"310-206-1016"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL6/J","AGE_OR_AGE_RANGE":"8-12","GENDER":"Male","CELL_PASSAGE_NUMBER":"Primary"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"NT_1",
"Factors":{"Treatment":"NT","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (1).mzML Sample_Method2 (1).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_2",
"Factors":{"Treatment":"NT","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (2).mzML Sample_Method2 (2).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_3",
"Factors":{"Treatment":"NT","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (3).mzML Sample_Method2 (3).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_4",
"Factors":{"Treatment":"NT","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (4).mzML Sample_Method2 (4).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_5",
"Factors":{"Treatment":"TLR2","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (5).mzML Sample_Method2 (5).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_6",
"Factors":{"Treatment":"TLR2","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (6).mzML Sample_Method2 (6).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_7",
"Factors":{"Treatment":"TLR2","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (7).mzML Sample_Method2 (7).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_8",
"Factors":{"Treatment":"TLR2","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (8).mzML Sample_Method2 (8).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_9",
"Factors":{"Treatment":"TLR3","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (9).mzML Sample_Method2 (9).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_10",
"Factors":{"Treatment":"TLR3","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (10).mzML Sample_Method2 (10).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_11",
"Factors":{"Treatment":"TLR3","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (11).mzML Sample_Method2 (11).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_12",
"Factors":{"Treatment":"TLR3","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (12).mzML Sample_Method2 (12).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_13",
"Factors":{"Treatment":"TLR4","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (13).mzML Sample_Method2 (13).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_14",
"Factors":{"Treatment":"TLR4","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (14).mzML Sample_Method2 (14).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_15",
"Factors":{"Treatment":"TLR4","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (15).mzML Sample_Method2 (15).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_16",
"Factors":{"Treatment":"TLR4","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (16).mzML Sample_Method2 (16).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR7_17",
"Factors":{"Treatment":"TLR7","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (17).mzML Sample_Method2 (17).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR7_18",
"Factors":{"Treatment":"TLR7","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (18).mzML Sample_Method2 (18).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR7_19",
"Factors":{"Treatment":"TLR7","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (19).mzML Sample_Method2 (19).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR7_20",
"Factors":{"Treatment":"TLR7","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (20).mzML Sample_Method2 (20).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR9_21",
"Factors":{"Treatment":"TLR9","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (21).mzML Sample_Method2 (21).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR9_22",
"Factors":{"Treatment":"TLR9","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (22).mzML Sample_Method2 (22).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR9_23",
"Factors":{"Treatment":"TLR9","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (23).mzML Sample_Method2 (23).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR9_24",
"Factors":{"Treatment":"TLR9","Time":"24"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (24).mzML Sample_Method2 (24).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_25",
"Factors":{"Treatment":"NT","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (25).mzML Sample_Method2 (25).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_26",
"Factors":{"Treatment":"NT","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (26).mzML Sample_Method2 (26).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_27",
"Factors":{"Treatment":"NT","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (27).mzML Sample_Method2 (27).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_28",
"Factors":{"Treatment":"NT","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (28).mzML Sample_Method2 (28).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_29",
"Factors":{"Treatment":"TLR2","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (29).mzML Sample_Method2 (29).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_30",
"Factors":{"Treatment":"TLR2","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (30).mzML Sample_Method2 (30).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_31",
"Factors":{"Treatment":"TLR2","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (31).mzML Sample_Method2 (31).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_32",
"Factors":{"Treatment":"TLR2","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (32).mzML Sample_Method2 (32).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_33",
"Factors":{"Treatment":"TLR3","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (33).mzML Sample_Method2 (33).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_34",
"Factors":{"Treatment":"TLR3","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (34).mzML Sample_Method2 (34).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_35",
"Factors":{"Treatment":"TLR3","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (35).mzML Sample_Method2 (35).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_36",
"Factors":{"Treatment":"TLR3","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (36).mzML Sample_Method2 (36).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_37",
"Factors":{"Treatment":"TLR4","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (37).mzML Sample_Method2 (37).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_38",
"Factors":{"Treatment":"TLR4","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (38).mzML Sample_Method2 (38).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_39",
"Factors":{"Treatment":"TLR4","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (39).mzML Sample_Method2 (39).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_40",
"Factors":{"Treatment":"TLR4","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (40).mzML Sample_Method2 (40).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR7_41",
"Factors":{"Treatment":"TLR7","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (41).mzML Sample_Method2 (41).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR7_42",
"Factors":{"Treatment":"TLR7","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (42).mzML Sample_Method2 (42).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR7_43",
"Factors":{"Treatment":"TLR7","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (43).mzML Sample_Method2 (43).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR7_44",
"Factors":{"Treatment":"TLR7","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (44).mzML Sample_Method2 (44).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR9_45",
"Factors":{"Treatment":"TLR9","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (45).mzML Sample_Method2 (45).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR9_46",
"Factors":{"Treatment":"TLR9","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (46).mzML Sample_Method2 (46).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR9_47",
"Factors":{"Treatment":"TLR9","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (47).mzML Sample_Method2 (47).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR9_48",
"Factors":{"Treatment":"TLR9","Time":"48"},
"Additional sample data":{"RAW_FILE_NAME":"Sample_Method1 (48).mzML Sample_Method2 (48).mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Day 6 BMDMs were seeded at a density of 9x105 cell/well in 6 well dishes (Fisher 08-772-1B). On day 8, BMDM were mock-treated (media change) or co-treated with ligands as described below in 5% BMDM media for 48 h. Prior to collection, cell number was assessed by imaging as described below. After removal of culturing media, 1 mL of ice-cold PBS was added into each well and cells were scraped with cell lifters and spun down in glass tubes at 365g for 5 min at 4°C.","SAMPLE_TYPE":"Macrophages","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"BMDMs were seeded at a density of 9x105 cell/well in 6 well dishes (Fisher 08-772-1B). On day 8, BMDM were mock-treated (media change) or co-treated with TLR2, TLR3, TLR4, TLR7, or TLR9 ligands in 5% FBS BMDM media for 24 or 48 h."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"A modified Bligh and Dyer extraction (Bligh and Dyer, 1959) was carried out on samples. Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10 mM Ammonium Acetate and transferred to robovials (Thermo 10800107) for analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"None (Direct infusion)","INSTRUMENT_NAME":"none","COLUMN_NAME":"none"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 5500 QTRAP with SelexION","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"Lipidyzer Workflow Manager 1.0 Samples are normalized to cell count"},

"MS_METABOLITE_DATA":{
"Units":"nmol per 10e7 cells",

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