{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001343","ANALYSIS_ID":"AN002237","VERSION":"1","CREATED_ON":"April 6, 2020, 3:17 pm"},

"PROJECT":{"PROJECT_TITLE":"Toll-like receptors induce signal specific reprogramming of the macrophage lipidome","PROJECT_TYPE":"Shotgun lipidomics","PROJECT_SUMMARY":"Macrophages reprogram their lipid metabolism in response to activation signals. However, a systems level understanding of how different pro-inflammatory stimuli reshape the macrophage lipidome is lacking. Herein, we use complementary “shotgun” mass spectrometry approaches to define the changes in lipid composition of macrophages induced by Toll-like receptors (TLRs) and inflammatory cytokines. “Shotgun” lipidomics data revealed that different TLRs and cytokines induce macrophages to acquire distinct lipidomes, indicating specificity in reshaping lipid composition.","INSTITUTE":"University of California, Los Angeles","DEPARTMENT":"MIMG","LABORATORY":"Bensinger lab","LAST_NAME":"Bensinger","FIRST_NAME":"Steven","ADDRESS":"University of California, Los Angeles","EMAIL":"bensingerlab@gmail.com","PHONE":"3102061016"},

"STUDY":{"STUDY_TITLE":"C56BL6 WT or TRIFKO BMDM stimulated with different TLRs (part-III)","STUDY_TYPE":"WT vs TRIF deficient macrophages","STUDY_SUMMARY":"WT control or TRIF deficient bone marrow derived macrophages were stimulated with TLR2, TLR3, and TLR4 ligands for 48 h.","INSTITUTE":"University of California, Los Angeles","DEPARTMENT":"MIMG","LABORATORY":"Bensinger lab","LAST_NAME":"Bensinger","FIRST_NAME":"Steven","ADDRESS":"University of California, Los Angeles","EMAIL":"bensingerlab@gmail.com","PHONE":"3102061016"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL6/J","AGE_OR_AGE_RANGE":"8-12","GENDER":"Male"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"NT_WT_1",
"Factors":{"Treatment":"NT","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (1).mzML Sample_Method2 (1).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_WT_2",
"Factors":{"Treatment":"NT","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (2).mzML Sample_Method2 (2).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_WT_3",
"Factors":{"Treatment":"NT","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (3).mzML Sample_Method2 (3).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_WT_4",
"Factors":{"Treatment":"NT","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (4).mzML Sample_Method2 (4).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_TRIFKO_1",
"Factors":{"Treatment":"NT","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (5).mzML Sample_Method2 (5).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_TRIFKO_2",
"Factors":{"Treatment":"NT","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (6).mzML Sample_Method2 (6).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_TRIFKO_3",
"Factors":{"Treatment":"NT","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (7).mzML Sample_Method2 (7).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"NT_TRIFKO_4",
"Factors":{"Treatment":"NT","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (8).mzML Sample_Method2 (8).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_WT_1",
"Factors":{"Treatment":"TLR2","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (9).mzML Sample_Method2 (9).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_WT_2",
"Factors":{"Treatment":"TLR2","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (10).mzML Sample_Method2 (10).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_WT_3",
"Factors":{"Treatment":"TLR2","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (11).mzML Sample_Method2 (11).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_WT_4",
"Factors":{"Treatment":"TLR2","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (12).mzML Sample_Method2 (12).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_TRIFKO_1",
"Factors":{"Treatment":"TLR2","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (13).mzML Sample_Method2 (13).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_TRIFKO_2",
"Factors":{"Treatment":"TLR2","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (14).mzML Sample_Method2 (14).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_TRIFKO_3",
"Factors":{"Treatment":"TLR2","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (15).mzML Sample_Method2 (15).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR2_TRIFKO_4",
"Factors":{"Treatment":"TLR2","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (16).mzML Sample_Method2 (16).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_ WT_1",
"Factors":{"Treatment":"TLR3","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (17).mzML Sample_Method2 (17).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_ WT_2",
"Factors":{"Treatment":"TLR3","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (18).mzML Sample_Method2 (18).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_ WT_4",
"Factors":{"Treatment":"TLR3","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (20).mzML Sample_Method2 (20).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_TRIFKO_1",
"Factors":{"Treatment":"TLR3","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (21).mzML Sample_Method2 (21).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_TRIFKO_2",
"Factors":{"Treatment":"TLR3","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (22).mzML Sample_Method2 (22).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_TRIFKO_3",
"Factors":{"Treatment":"TLR3","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (23).mzML Sample_Method2 (23).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR3_TRIFKO_4",
"Factors":{"Treatment":"TLR3","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (24).mzML Sample_Method2 (24).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_WT_1",
"Factors":{"Treatment":"TLR4","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (25).mzML Sample_Method2 (25).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_WT_2",
"Factors":{"Treatment":"TLR4","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (26).mzML Sample_Method2 (26).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_WT_3",
"Factors":{"Treatment":"TLR4","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (27).mzML Sample_Method2 (27).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_WT_4",
"Factors":{"Treatment":"TLR4","Genotype":"WT"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (28).mzML Sample_Method2 (28).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_TRIFKO_2",
"Factors":{"Treatment":"TLR4","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (30).mzML Sample_Method2 (30).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_TRIFKO_3",
"Factors":{"Treatment":"TLR4","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (31).mzML Sample_Method2 (31).mzML"}
},
{
"Subject ID":"-",
"Sample ID":"TLR4_TRIFKO_4",
"Factors":{"Treatment":"TLR4","Genotype":"TRIF-/-"},
"Additional sample data":{"Time":"48","RAW_FILE_NAME":"Sample_Method1 (32).mzML Sample_Method2 (32).mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Day 6 BMDMs were seeded at a density of 9x105 cell/well in 6 well dishes (Fisher 08-772-1B). On day 8, BMDM were mock-treated (media change) or co-treated with ligands as described below in 5% BMDM media for 48 h. Prior to collection, cell number was assessed by imaging as described below. After removal of culturing media, 1 mL of ice-cold PBS was added into each well and cells were scraped with cell lifters and spun down in glass tubes at 365g for 5 min at 4°C.","SAMPLE_TYPE":"Macrophages","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"WT or TRIF deficient BMDMs were seeded at a density of 9x105 cell/well in 6 well dishes (Fisher 08-772-1B). On day 8, BMDM were stimulated with TLR2, TLR3, or TLR4 ligands in 5% FBS BMDM media for 48 h."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"A modified Bligh and Dyer extraction (Bligh and Dyer, 1959) was carried out on samples. Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10 mM Ammonium Acetate and transferred to robovials (Thermo 10800107) for analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"None (Direct infusion)","INSTRUMENT_NAME":"none","COLUMN_NAME":"none"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 5500 QTRAP with SelexION","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"Lipidyzer Workflow Manager 1.0 Samples are normalized to cell count"},

"MS_METABOLITE_DATA":{
"Units":"nmol per 10e7 cells",

"Data":[{"Metabolite":"CE(14:0)","NT_WT_1":"0.026814102","NT_WT_2":"0","NT_WT_3":"0.021111208","NT_WT_4":"0.026530626","NT_TRIFKO_1":"0.028807188","NT_TRIFKO_2":"0.0275723","NT_TRIFKO_3":"0.026032652","NT_TRIFKO_4":"0","TLR2_WT_1":"0.028131583","TLR2_WT_2":"0.034547286","TLR2_WT_3":"0","TLR2_WT_4":"0","TLR2_TRIFKO_1":"0","TLR2_TRIFKO_2":"0","TLR2_TRIFKO_3":"0","TLR2_TRIFKO_4":"0","TLR3_ WT_1":"0.559725962","TLR3_ WT_2":"0.545330438","TLR3_ WT_4":"0.468602737","TLR3_TRIFKO_1":"0.088087415","TLR3_TRIFKO_2":"0.100424868","TLR3_TRIFKO_3":"0.082196403","TLR3_TRIFKO_4":"0.076969256","TLR4_WT_1":"0.363152645","TLR4_WT_2":"0.322034501","TLR4_WT_3":"0.369376993","TLR4_WT_4":"0.40995695","TLR4_TRIFKO_2":"0.039954088","TLR4_TRIFKO_3":"0.049096708","TLR4_TRIFKO_4":"0.038408763"},{"Metabolite":"CE(15:0)","NT_WT_1":"0","NT_WT_2":"0","NT_WT_3":"0","NT_WT_4":"0","NT_TRIFKO_1":"0","NT_TRIFKO_2":"0","NT_TRIFKO_3":"0","NT_TRIFKO_4":"0","TLR2_WT_1":"0","TLR2_WT_2":"0","TLR2_WT_3":"0","TLR2_WT_4":"0","TLR2_TRIFKO_1":"0","TLR2_TRIFKO_2":"0","TLR2_TRIFKO_3":"0","TLR2_TRIFKO_4":"0","TLR3_ WT_1":"0.166667708","TLR3_ WT_2":"0.147996389","TLR3_ WT_4":"0.168798982","TLR3_TRIFKO_1":"0.031746001","TLR3_TRIFKO_2":"0.040058697","TLR3_TRIFKO_3":"0","TLR3_TRIFKO_4":"0.033351379","TLR4_WT_1":"0.085966922","TLR4_WT_2":"0.094972011","TLR4_WT_3":"0.107459225","TLR4_WT_4":"0.138460521","TLR4_TRIFKO_2":"0","TLR4_TRIFKO_3":"0","TLR4_TRIFKO_4":"0"},{"Metabolite":"CE(16:0)","NT_WT_1":"0.181131241","NT_WT_2":"0.113908477","NT_WT_3":"0.186835533","NT_WT_4":"0.213752096","NT_TRIFKO_1":"0.199930038","NT_TRIFKO_2":"0.212131492","NT_TRIFKO_3":"0.23223449","NT_TRIFKO_4":"0.147910452","TLR2_WT_1":"0.245806214","TLR2_WT_2":"0.224803697","TLR2_WT_3":"0.223441926","TLR2_WT_4":"0.212175036","TLR2_TRIFKO_1":"0.184895333","TLR2_TRIFKO_2":"0.157677297","TLR2_TRIFKO_3":"0.156466166","TLR2_TRIFKO_4":"0.1536322","TLR3_ WT_1":"1.899664459","TLR3_ WT_2":"1.928753895","TLR3_ WT_4":"1.704821292","TLR3_TRIFKO_1":"0.443629592","TLR3_TRIFKO_2":"0.485825384","TLR3_TRIFKO_3":"0.442663655","TLR3_TRIFKO_4":"0.436431429","TLR4_WT_1":"1.042051449","TLR4_WT_2":"0.979923701","TLR4_WT_3":"1.083180362","TLR4_WT_4":"1.419929179","TLR4_TRIFKO_2":"0.265287567","TLR4_TRIFKO_3":"0.209875112","TLR4_TRIFKO_4":"0.228667286"},{"Metabolite":"CE(16:1)","NT_WT_1":"0.058848368","NT_WT_2":"0.036167868","NT_WT_3":"0.061380771","NT_WT_4":"0.077257373","NT_TRIFKO_1":"0.067120868","NT_TRIFKO_2":"0.087231428","NT_TRIFKO_3":"0.067783464","NT_TRIFKO_4":"0.045829261","TLR2_WT_1":"0.083195253","TLR2_WT_2":"0.085129051","TLR2_WT_3":"0.074824486","TLR2_WT_4":"0.06051631","TLR2_TRIFKO_1":"0.053107756","TLR2_TRIFKO_2":"0.048717426","TLR2_TRIFKO_3":"0.051348418","TLR2_TRIFKO_4":"0.069253301","TLR3_ WT_1":"0.425866781","TLR3_ WT_2":"0.44074909","TLR3_ WT_4":"0.382152436","TLR3_TRIFKO_1":"0.154649771","TLR3_TRIFKO_2":"0.15650881","TLR3_TRIFKO_3":"0.136962701","TLR3_TRIFKO_4":"0.146004926","TLR4_WT_1":"0.250600166","TLR4_WT_2":"0.236664897","TLR4_WT_3":"0.230045682","TLR4_WT_4":"0.302577193","TLR4_TRIFKO_2":"0.106110293","TLR4_TRIFKO_3":"0.063031463","TLR4_TRIFKO_4":"0.089339583"},{"Metabolite":"CE(17:0)","NT_WT_1":"0","NT_WT_2":"0","NT_WT_3":"0","NT_WT_4":"0","NT_TRIFKO_1":"0","NT_TRIFKO_2":"0","NT_TRIFKO_3":"0.022969987","NT_TRIFKO_4":"0","TLR2_WT_1":"0","TLR2_WT_2":"0","TLR2_WT_3":"0","TLR2_WT_4":"0","TLR2_TRIFKO_1":"0","TLR2_TRIFKO_2":"0","TLR2_TRIFKO_3":"0","TLR2_TRIFKO_4":"0","TLR3_ WT_1":"0.17772424","TLR3_ WT_2":"0.189011006","TLR3_ WT_4":"0.163975872","TLR3_TRIFKO_1":"0.032625572","TLR3_TRIFKO_2":"0","TLR3_TRIFKO_3":"0.034251347","TLR3_TRIFKO_4":"0","TLR4_WT_1":"0.095609401","TLR4_WT_2":"0.089788242","TLR4_WT_3":"0.098822096","TLR4_WT_4":"0.126695708","TLR4_TRIFKO_2":"0","TLR4_TRIFKO_3":"0","TLR4_TRIFKO_4":"0"},{"Metabolite":"CE(18:0)","NT_WT_1":"0.036387925","NT_WT_2":"0","NT_WT_3":"0.035285493","NT_WT_4":"0.036802453","NT_TRIFKO_1":"0.036449545","NT_TRIFKO_2":"0.035442762","NT_TRIFKO_3":"0.045423663","NT_TRIFKO_4":"0","TLR2_WT_1":"0.039017578","TLR2_WT_2":"0","TLR2_WT_3":"0.037227491","TLR2_WT_4":"0.029717971","TLR2_TRIFKO_1":"0.030338723","TLR2_TRIFKO_2":"0","TLR2_TRIFKO_3":"0","TLR2_TRIFKO_4":"0","TLR3_ WT_1":"0.975638937","TLR3_ WT_2":"0.990919885","TLR3_ WT_4":"0.844775702","TLR3_TRIFKO_1":"0.117455316","TLR3_TRIFKO_2":"0.122745429","TLR3_TRIFKO_3":"0.113878045","TLR3_TRIFKO_4":"0.095447267","TLR4_WT_1":"0.359938485","TLR4_WT_2":"0.359162532","TLR4_WT_3":"0.398160889","TLR4_WT_4":"0.49955658","TLR4_TRIFKO_2":"0.047054932","TLR4_TRIFKO_3":"0","TLR4_TRIFKO_4":"0.044782415"},{"Metabolite":"CE(18:1)","NT_WT_1":"0.227003169","NT_WT_2":"0.137683669","NT_WT_3":"0.221986902","NT_WT_4":"0.254437512","NT_TRIFKO_1":"0.281040914","NT_TRIFKO_2":"0.273948006","NT_TRIFKO_3":"0.277963246","NT_TRIFKO_4":"0.190837626","TLR2_WT_1":"0.262561442","TLR2_WT_2":"0.248407525","TLR2_WT_3":"0.255149957","TLR2_WT_4":"0.238518811","TLR2_TRIFKO_1":"0.191370792","TLR2_TRIFKO_2":"0.183915875","TLR2_TRIFKO_3":"0.17031998","TLR2_TRIFKO_4":"0.190676966","TLR3_ WT_1":"5.675240863","TLR3_ WT_2":"5.869575096","TLR3_ WT_4":"4.925650962","TLR3_TRIFKO_1":"0.878268014","TLR3_TRIFKO_2":"0.937348798","TLR3_TRIFKO_3":"0.821084692","TLR3_TRIFKO_4":"0.857231505","TLR4_WT_1":"2.197650914","TLR4_WT_2":"2.193767388","TLR4_WT_3":"2.213889446","TLR4_WT_4":"2.8046033","TLR4_TRIFKO_2":"0.371200614","TLR4_TRIFKO_3":"0.279689879","TLR4_TRIFKO_4":"0.326833209"},{"Metabolite":"CE(18:2)","NT_WT_1":"0.072453553","NT_WT_2":"0.040255368","NT_WT_3":"0.065849802","NT_WT_4":"0.074030435","NT_TRIFKO_1":"0.073995992","NT_TRIFKO_2":"0.07865896","NT_TRIFKO_3":"0.076502085","NT_TRIFKO_4":"0.048595842","TLR2_WT_1":"0.06296224","TLR2_WT_2":"0.074297566","TLR2_WT_3":"0.084185244","TLR2_WT_4":"0.073833029","TLR2_TRIFKO_1":"0.05486902","TLR2_TRIFKO_2":"0.052491617","TLR2_TRIFKO_3":"0.038491016","TLR2_TRIFKO_4":"0.056252599","TLR3_ WT_1":"0.419611497","TLR3_ WT_2":"0.447831816","TLR3_ WT_4":"0.40174138","TLR3_TRIFKO_1":"0.126495352","TLR3_TRIFKO_2":"0.118410225","TLR3_TRIFKO_3":"0.111564457","TLR3_TRIFKO_4":"0.115281231","TLR4_WT_1":"0.161352716","TLR4_WT_2":"0.130598471","TLR4_WT_3":"0.142556755","TLR4_WT_4":"0.199873826","TLR4_TRIFKO_2":"0.094212433","TLR4_TRIFKO_3":"0.078446639","TLR4_TRIFKO_4":"0.07797713"},{"Metabolite":"CE(18:3)","NT_WT_1":"0.009309645","NT_WT_2":"0","NT_WT_3":"0","NT_WT_4":"0","NT_TRIFKO_1":"0","NT_TRIFKO_2":"0.0093715","NT_TRIFKO_3":"0","NT_TRIFKO_4":"0","TLR2_WT_1":"0","TLR2_WT_2":"0","TLR2_WT_3":"0","TLR2_WT_4":"0","TLR2_TRIFKO_1":"0","TLR2_TRIFKO_2":"0","TLR2_TRIFKO_3":"0","TLR2_TRIFKO_4":"0","TLR3_ WT_1":"0","TLR3_ WT_2":"0","TLR3_ 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