{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001351","ANALYSIS_ID":"AN002247","VERSION":"1","CREATED_ON":"April 7, 2020, 5:33 pm"},

"PROJECT":{"PROJECT_TITLE":"NMR Metabolomic Analysis of Bacterial Resistance Pathways Using Multivalent Quaternary Ammonium Functionalized Macromolecules","PROJECT_TYPE":"NMR Hydrophilic Metabolomic Analysis","PROJECT_SUMMARY":"Multivalent antimicrobial dendrimers are an exciting new system that is being developed to address the growing problem of drug resistant bacteria. Nuclear Magnetic Resonance (NMR) metabolomics is a quantitative and reproducible method for the determination of bacterial response to environmental stressors and for visualization of perturbations to biochemical pathways. NMR metabolomics is used to elucidate metabolite differences between wild type and antimicrobially mutated Escherichia coli (E. coli) samples. Proton (1H) NMR hydrophilic metabolite analysis was conducted on samples of E. coli after 33 growth cycles of a minimum inhibitory challenge to E. coli by poly(amidoamine) dendrimers functionalized with mannose and with C16-DABCO quaternary ammonium endgroups and compared to the metabolic profile of wild type E. coli. The wild type and mutated E. coli samples were separated into distinct sample sets by hierarchical clustering, principal component analysis (PCA) and sparse partial least squares discriminate analysis (sPLS-DA). Metabolite components of membrane fortification and energy related pathways had a significant p-value and fold change between the wild type and mutated E. coli. Amino acids commonly associated with membrane fortification from cationic antimicrobials, such as lysine, were found to have a higher concentration in the mutated E. coli than the wild type E. coli. N-acetylglucosamine, a major component of peptidoglycan synthesis, was found to have a 25 fold higher concentration in the mid log phase of the mutated E. coli than the mid log phase of the wild type. The metabolic profile suggests that E. coli change their peptidoglycan composition in order to garner protection from the highly positively charged and multivalent C16 DABCO and mannose functionalized dendrimer.","INSTITUTE":"Montana State University","DEPARTMENT":"Chemistry and Biochemistry","LABORATORY":"Cloninger","LAST_NAME":"Aries","FIRST_NAME":"Michelle","ADDRESS":"Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, 59717 USA","EMAIL":"michelle.aries@montana.edu","PHONE":"4069943051"},

"STUDY":{"STUDY_TITLE":"NMR Metabolomic Analysis of Bacterial Resistance Pathways Using Multivalent Quaternary Ammonium Functionalized Macromolecules","STUDY_TYPE":"NMR Hydrophilic Metabolomics","STUDY_SUMMARY":"Multivalent antimicrobial dendrimers are an exciting new system that is being developed to address the growing problem of drug resistant bacteria. Nuclear Magnetic Resonance (NMR) metabolomics is a quantitative and reproducible method for the determination of bacterial response to environmental stressors and for visualization of perturbations to biochemical pathways. NMR metabolomics is used to elucidate metabolite differences between wild type and antimicrobially mutated Escherichia coli (E. coli) samples. Proton (1H) NMR hydrophilic metabolite analysis was conducted on samples of E. coli after 33 growth cycles of a minimum inhibitory challenge to E. coli by poly(amidoamine) dendrimers functionalized with mannose and with C16-DABCO quaternary ammonium endgroups and compared to the metabolic profile of wild type E. coli. The wild type and mutated E. coli samples were separated into distinct sample sets by hierarchical clustering, principal component analysis (PCA) and sparse partial least squares discriminate analysis (sPLS-DA). Metabolite components of membrane fortification and energy related pathways had a significant p-value and fold change between the wild type and mutated E. coli. Amino acids commonly associated with membrane fortification from cationic antimicrobials, such as lysine, were found to have a higher concentration in the mutated E. coli than the wild type E. coli. N-acetylglucosamine, a major component of peptidoglycan synthesis, was found to have a 25 fold higher concentration in the mid log phase of the mutated E. coli than the mid log phase of the wild type.The metabolic profile suggests that E. coli change their peptidoglycan composition in order to garner protection from the highly positively charged and multivalent C16-DABCO and mannose functionalized dendrimer.","INSTITUTE":"Montana State University","DEPARTMENT":"Chemistry and Biochemistry","LABORATORY":"Cloninger","LAST_NAME":"Aries","FIRST_NAME":"Michelle","ADDRESS":"Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, 59717 USA","EMAIL":"michelle.aries@montana.edu","PHONE":"4069943051"},

"SUBJECT":{"SUBJECT_TYPE":"Bacteria","SUBJECT_SPECIES":"Escherichia coli","TAXONOMY_ID":"562","GENOTYPE_STRAIN":"ATCC 25922"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"WT_S_1",
"Factors":{"Genotype":"Wild Type","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"fid - WT_2_1 - S"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_2",
"Factors":{"Genotype":"Wild Type","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"fid - WT_2_2 - S"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_5",
"Factors":{"Genotype":"Wild Type","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_6",
"Factors":{"Genotype":"Wild Type","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_7",
"Factors":{"Genotype":"Wild Type","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_9",
"Factors":{"Genotype":"Wild Type","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"fid - WT_2_9 - S"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_10",
"Factors":{"Genotype":"Wild Type","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"fid - WT_2_10 - S"}
},
{
"Subject ID":"-",
"Sample ID":"Mut_S_1",
"Factors":{"Genotype":"Mutant","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut_S_2",
"Factors":{"Genotype":"Mutant","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"fid - Mut_2_2 - S"}
},
{
"Subject ID":"-",
"Sample ID":"Mut_S_3",
"Factors":{"Genotype":"Mutant","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut_S_7",
"Factors":{"Genotype":"Mutant","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut_S_8",
"Factors":{"Genotype":"Mutant","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut_S_9",
"Factors":{"Genotype":"Mutant","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"fid - Mut_2_9 - S"}
},
{
"Subject ID":"-",
"Sample ID":"Mut_S_10",
"Factors":{"Genotype":"Mutant","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut_S_12",
"Factors":{"Genotype":"Mutant","Phase":"Stationary"},
"Additional sample data":{"RAW_FILE_NAME":"fid - Mut_2_12 - S"}
},
{
"Subject ID":"-",
"Sample ID":"Mut _ML_1",
"Factors":{"Genotype":"Mutant","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"fid - Mut_1_2 - ML"}
},
{
"Subject ID":"-",
"Sample ID":"Mut _ML_2",
"Factors":{"Genotype":"Mutant","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut _ML_3",
"Factors":{"Genotype":"Mutant","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut _ML_4",
"Factors":{"Genotype":"Mutant","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"fid - Mut_1_4 - ML"}
},
{
"Subject ID":"-",
"Sample ID":"Mut _ML_6",
"Factors":{"Genotype":"Mutant","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut _ML_7",
"Factors":{"Genotype":"Mutant","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut _ML_8",
"Factors":{"Genotype":"Mutant","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut _ML_9",
"Factors":{"Genotype":"Mutant","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"Mut _ML_10",
"Factors":{"Genotype":"Mutant","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"fid - Mut_1_10 - ML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_1",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"fid - WT_1_1 - ML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_2",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"fid - WT_1_2 - ML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_3",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_6",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_7",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_8",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"fid - WT_1_8 - ML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_9",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_10",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_11",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_12",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"-"}
},
{
"Subject ID":"-",
"Sample ID":"WT_ML_13",
"Factors":{"Genotype":"Wild Type","Phase":"Mid Log"},
"Additional sample data":{"RAW_FILE_NAME":"fid - WT_1_13 -ML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Samples were collected in the mid log and stationary phases, washed with cold PBS, frozen at -80 ºC. Please see attached PDF for detailed procedures.","SAMPLE_TYPE":"Bacterial cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"The samples were not treated with anything. I collected the samples then extracted the hydrophilic metabolites, placed them in an NMR buffer then ran them on the NMR. All these procedures were uploaded."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"The hydrophilic metabolites were extracted, the samples were added to an NMR buffer, run on the NMR and profiled, standardized to their concentrations and analyzed. See uploaded protocol for more details."},

"ANALYSIS":{"ANALYSIS_TYPE":"NMR"},

"NM":{"INSTRUMENT_NAME":"Buker Avance III","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","SPECTROMETER_FREQUENCY":"600 MHz"},

"NMR_METABOLITE_DATA":{
"Units":"mM",

"Data":[{"Metabolite":"Acetate","WT_S_1":"5.004207","WT_S_2":"1.420349","WT_S_5":"3.673355","WT_S_6":"2.396972","WT_S_7":"2.696732","WT_S_9":"2.342503","WT_S_10":"3.050962","Mut_S_1":"2.195773","Mut_S_2":"0.663715","Mut_S_3":"0.3064","Mut_S_7":"1.279346","Mut_S_8":"1.157738","Mut_S_9":"1.651761","Mut_S_10":"0.362918","Mut_S_12":"0.892798","Mut _ML_1":"4.604748","Mut _ML_2":"3.940933","Mut _ML_3":"2.762371","Mut _ML_4":"3.306119","Mut _ML_6":"2.218623","Mut _ML_7":"4.01517","Mut _ML_8":"3.520154","Mut _ML_9":"3.1311","Mut _ML_10":"4.27284","WT_ML_1":"3.696428","WT_ML_2":"4.507632","WT_ML_3":"6.053971","WT_ML_6":"4.605458","WT_ML_7":"5.477735","WT_ML_8":"5.041596","WT_ML_9":"4.587081","WT_ML_10":"4.10203","WT_ML_11":"5.280802","WT_ML_12":"5.514488","WT_ML_13":"4.901498"},{"Metabolite":"Acetoacetate","WT_S_1":"0.24309","WT_S_2":"0.123455","WT_S_5":"0.283431","WT_S_6":"0.10069","WT_S_7":"0.306197","WT_S_9":"0.323773","WT_S_10":"0.288621","Mut_S_1":"0.139559","Mut_S_2":"0.066026","Mut_S_3":"0.225876","Mut_S_7":"0.14557","Mut_S_8":"0.103174","Mut_S_9":"0.140322","Mut_S_10":"0.151581","Mut_S_12":"0.11931","Mut _ML_1":"0.671179","Mut _ML_2":"0.081061","Mut _ML_3":"0.421237","Mut _ML_4":"0.272755","Mut _ML_6":"0.56972","Mut _ML_7":"0.296206","Mut _ML_8":"0.258009","Mut _ML_9":"0.278807","Mut _ML_10":"0.37612","WT_ML_1":"0","WT_ML_2":"0.159002","WT_ML_3":"0.052112","WT_ML_6":"0","WT_ML_7":"0.084259","WT_ML_8":"0.042129","WT_ML_9":"0.042129","WT_ML_10":"0.079501","WT_ML_11":"0.105557","WT_ML_12":"0","WT_ML_13":"0.116406"},{"Metabolite":"Adenosine","WT_S_1":"0.61653","WT_S_2":"0.066666","WT_S_5":"0.497739","WT_S_6":"0.086758","WT_S_7":"0.477647","WT_S_9":"0.378948","WT_S_10":"0.576345","Mut_S_1":"0.936612","Mut_S_2":"0.878938","Mut_S_3":"0.978256","Mut_S_7":"0.967761","Mut_S_8":"0.522493","Mut_S_9":"0.166048","Mut_S_10":"0.99891","Mut_S_12":"0.681298","Mut _ML_1":"0.638963","Mut _ML_2":"0.890187","Mut _ML_3":"0.385446","Mut _ML_4":"0.393061","Mut _ML_6":"0.377831","Mut _ML_7":"0.604839","Mut _ML_8":"0.711331","Mut _ML_9":"0.335407","Mut _ML_10":"0.764575","WT_ML_1":"0.707489","WT_ML_2":"0.428092","WT_ML_3":"0.272013","WT_ML_6":"0.491446","WT_ML_7":"0.291119","WT_ML_8":"0.391282","WT_ML_9":"0.499304","WT_ML_10":"0.567791","WT_ML_11":"0.350053","WT_ML_12":"0.275403","WT_ML_13":"0.310225"},{"Metabolite":"Alanine","WT_S_1":"3.095242","WT_S_2":"3.603872","WT_S_5":"3.238241","WT_S_6":"3.640412","WT_S_7":"3.201702","WT_S_9":"3.381241","WT_S_10":"3.022163","Mut_S_1":"3.745169","Mut_S_2":"2.799352","Mut_S_3":"3.728143","Mut_S_7":"3.126668","Mut_S_8":"3.661522","Mut_S_9":"4.523691","Mut_S_10":"2.508166","Mut_S_12":"3.27707","Mut _ML_1":"3.633135","Mut _ML_2":"4.2206","Mut _ML_3":"4.285751","Mut _ML_4":"4.944485","Mut _ML_6":"3.627018","Mut _ML_7":"4.11156","Mut _ML_8":"4.209","Mut _ML_9":"4.383348","Mut _ML_10":"3.926867","WT_ML_1":"5.898304","WT_ML_2":"5.433286","WT_ML_3":"5.261134","WT_ML_6":"5.754285","WT_ML_7":"5.142107","WT_ML_8":"5.448196","WT_ML_9":"5.520206","WT_ML_10":"5.665795","WT_ML_11":"5.34721","WT_ML_12":"5.610267","WT_ML_13":"5.023081"},{"Metabolite":"AMP","WT_S_1":"0.667406","WT_S_2":"0.900793","WT_S_5":"0.625175","WT_S_6":"1.012276","WT_S_7":"0.513692","WT_S_9":"0.582944","WT_S_10":"0.444441","Mut_S_1":"1.021736","Mut_S_2":"0.967562","Mut_S_3":"0.70847","Mut_S_7":"0.855155","Mut_S_8":"1.329448","Mut_S_9":"1.691333","Mut_S_10":"0.688573","Mut_S_12":"1.115823","Mut _ML_1":"1.220935","Mut _ML_2":"0.583634","Mut _ML_3":"0.543861","Mut _ML_4":"0.529897","Mut _ML_6":"0.557824","Mut _ML_7":"0.884384","Mut _ML_8":"0.560594","Mut _ML_9":"0.696222","Mut _ML_10":"0.902284","WT_ML_1":"0.267592","WT_ML_2":"0.631895","WT_ML_3":"0.274798","WT_ML_6":"0.265641","WT_ML_7":"0.448609","WT_ML_8":"0.357125","WT_ML_9":"0.3581","WT_ML_10":"0.449743","WT_ML_11":"0.453346","WT_ML_12":"0.263691","WT_ML_13":"0.62242"},{"Metabolite":"Aspartate","WT_S_1":"6.022608","WT_S_2":"4.25488","WT_S_5":"5.513727","WT_S_6":"5.45505","WT_S_7":"4.313557","WT_S_9":"5.004846","WT_S_10":"3.622267","Mut_S_1":"7.298124","Mut_S_2":"5.442504","Mut_S_3":"6.527577","Mut_S_7":"7.484072","Mut_S_8":"5.890425","Mut_S_9":"6.338346","Mut_S_10":"7.67002","Mut_S_12":"6.483623","Mut _ML_1":"7.758444","Mut _ML_2":"12.54406","Mut _ML_3":"6.876525","Mut _ML_4":"9.075297","Mut _ML_6":"4.677754","Mut _ML_7":"10.43766","Mut _ML_8":"10.41235","Mut _ML_9":"8.943502","Mut _ML_10":"10.15125","WT_ML_1":"5.573016","WT_ML_2":"8.877851","WT_ML_3":"5.391347","WT_ML_6":"5.582218","WT_ML_7":"6.955479","WT_ML_8":"6.268848","WT_ML_9":"6.264247","WT_ML_10":"7.225434","WT_ML_11":"7.134599","WT_ML_12":"5.59142","WT_ML_13":"8.519611"},{"Metabolite":"Betaine","WT_S_1":"0.409897","WT_S_2":"0.624221","WT_S_5":"0.417007","WT_S_6":"0.562086","WT_S_7":"0.479143","WT_S_9":"0.424118","WT_S_10":"0.534167","Mut_S_1":"0.459575","Mut_S_2":"0.767859","Mut_S_3":"0.47037","Mut_S_7":"0.599755","Mut_S_8":"0.589995","Mut_S_9":"0.412131","Mut_S_10":"0.739935","Mut_S_12":"0.639975","Mut _ML_1":"0.759292","Mut _ML_2":"0.694907","Mut _ML_3":"0.922818","Mut _ML_4":"0.982652","Mut _ML_6":"0.862985","Mut _ML_7":"0.825154","Mut _ML_8":"0.769228","Mut _ML_9":"0.972041","Mut _ML_10":"0.727099","WT_ML_1":"0.45394","WT_ML_2":"0.555435","WT_ML_3":"0.345407","WT_ML_6":"0.398049","WT_ML_7":"0.394515","WT_ML_8":"0.396282","WT_ML_9":"0.424227","WT_ML_10":"0.504687","WT_ML_11":"0.450421","WT_ML_12":"0.342158","WT_ML_13":"0.443623"},{"Metabolite":"Cysteine","WT_S_1":"1.321165","WT_S_2":"2.693369","WT_S_5":"2.020742","WT_S_6":"2.719391","WT_S_7":"1.99472","WT_S_9":"2.72032","WT_S_10":"1.26912","Mut_S_1":"2.033308","Mut_S_2":"1.614617","Mut_S_3":"2.668459","Mut_S_7":"1.862824","Mut_S_8":"2.209791","Mut_S_9":"2.804966","Mut_S_10":"1.692341","Mut_S_12":"2.037308","Mut _ML_1":"2.183831","Mut _ML_2":"1.398319","Mut _ML_3":"2.489392","Mut _ML_4":"1.777957","Mut _ML_6":"3.200827","Mut _ML_7":"1.671801","Mut _ML_8":"1.869338","Mut _ML_9":"1.961323","Mut _ML_10":"1.791075","WT_ML_1":"3.417311","WT_ML_2":"2.66579","WT_ML_3":"3.087591","WT_ML_6":"3.04343","WT_ML_7":"3.354397","WT_ML_8":"3.198914","WT_ML_9":"3.385854","WT_ML_10":"3.04155","WT_ML_11":"2.876691","WT_ML_12":"2.669549","WT_ML_13":"3.621203"},{"Metabolite":"Cytosine","WT_S_1":"0.462037","WT_S_2":"0.195059","WT_S_5":"0.280464","WT_S_6":"0.278467","WT_S_7":"0.197057","WT_S_9":"0.098891","WT_S_10":"0.295222","Mut_S_1":"0.358148","Mut_S_2":"0.608798","Mut_S_3":"0.179239","Mut_S_7":"0.341424","Mut_S_8":"0.496371","Mut_S_9":"0.383944","Mut_S_10":"0.324701","Mut_S_12":"0.439148","Mut _ML_1":"0","Mut _ML_2":"0.180636","Mut _ML_3":"0.099953","Mut _ML_4":"0","Mut _ML_6":"0.199906","Mut _ML_7":"0.060212","Mut _ML_8":"0.153741","Mut _ML_9":"0.049977","Mut 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_ML_10":"2.75958","WT_ML_1":"3.790603","WT_ML_2":"4.342158","WT_ML_3":"3.485258","WT_ML_6":"3.693336","WT_ML_7":"3.434798","WT_ML_8":"3.564067","WT_ML_9":"3.612701","WT_ML_10":"4.066381","WT_ML_11":"3.913708","WT_ML_12":"3.596068","WT_ML_13":"3.384339"},{"Metabolite":"UDP-glucose","WT_S_1":"0.547829","WT_S_2":"0.462892","WT_S_5":"0.273914","WT_S_6":"0.542365","WT_S_7":"0.194442","WT_S_9":"0","WT_S_10":"0.388884","Mut_S_1":"1.029476","Mut_S_2":"0.648644","Mut_S_3":"0.517398","Mut_S_7":"0.956103","Mut_S_8":"0.927882","Mut_S_9":"1.20712","Mut_S_10":"0.88273","Mut_S_12":"0.912831","Mut _ML_1":"0.29204","Mut _ML_2":"0.672094","Mut _ML_3":"0.353843","Mut _ML_4":"0.269081","Mut _ML_6":"0.438604","Mut _ML_7":"0.485894","Mut _ML_8":"0.553017","Mut _ML_9":"0.423696","Mut 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}

}