{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001373","ANALYSIS_ID":"AN002293","VERSION":"1","CREATED_ON":"May 8, 2020, 12:11 pm"},

"PROJECT":{"PROJECT_TITLE":"Targeting Sirt2 reprograms T cell metabolism for effective immune response","PROJECT_TYPE":"Targeted Metabolomics","PROJECT_SUMMARY":"There is a growing evidence that metabolism is a key driver of T cell functions. A switch from oxidative phosphorylation to aerobic glycolysis is a hallmark of T cell activation and is required to meet metabolic demands of proliferation and effector functions. However the mechanisms underlying the metabolic switch in T cells remain unclear. Here we identify Sirt2 as a crucial immune checkpoint coordinating metabolic and functional fitness of T cells. Sirt2 is induced upon T cells activation and increases in late maturation stages. Sirt2 negatively regulates glycolysis by targeting key glycolytic enzymes. Remarkably, Sirt2 knockout T cells exhibit profound upregulation of aerobic glycolysis with enhanced proliferation and effector function and thus effectively reject tumor challenge in vivo. Furthermore pharmacologic inhibition of Sirt2 in human tumor infiltrating lymphocytes demonstrated similar phenotype. Taken together our results demonstrate Sirt2 as an actionable target to reprogram T cell metabolism to augment immunotherapy.","INSTITUTE":"Moffitt Cancer Center","DEPARTMENT":"Immunology","LABORATORY":"Sungjune Kim","LAST_NAME":"Koomen","FIRST_NAME":"John","ADDRESS":"12902 Magnolia Drive","EMAIL":"john.koomen@moffitt.org","PHONE":"8137458524","CONTRIBUTORS":"Imene Hamaidi1, Lin Zhang2, Nayoung Kim3, Min Hsuan Wang1, Cristina Iclozan1, Bin Fang4, Min Liu4, John Koomen4, Anders Berglund5, Sungjune Kim1,6*"},

"STUDY":{"STUDY_TITLE":"Targeting Sirt2 reprograms T cell metabolism for effective immune response","STUDY_TYPE":"Targeted Metabolomics","STUDY_SUMMARY":"There is a growing evidence that metabolism is a key driver of T cell functions. A switch from oxidative phosphorylation to aerobic glycolysis is a hallmark of T cell activation and is required to meet metabolic demands of proliferation and effector functions. However the mechanisms underlying the metabolic switch in T cells remain unclear. Here we identify Sirt2 as a crucial immune checkpoint coordinating metabolic and functional fitness of T cells. Sirt2 is induced upon T cells activation and increases in late maturation stages. Sirt2 negatively regulates glycolysis by targeting key glycolytic enzymes. Remarkably, Sirt2 knockout T cells exhibit profound upregulation of aerobic glycolysis with enhanced proliferation and effector function and thus effectively reject tumor challenge in vivo. Furthermore pharmacologic inhibition of Sirt2 in human tumor infiltrating lymphocytes demonstrated similar phenotype. Taken together our results demonstrate Sirt2 as an actionable target to reprogram T cell metabolism to augment immunotherapy.","INSTITUTE":"Moffitt Cancer Center","DEPARTMENT":"Immunology","LABORATORY":"Sungjune Kim","LAST_NAME":"Koomen","FIRST_NAME":"John","ADDRESS":"12902 Magnolia Drive","EMAIL":"john.koomen@moffitt.org","PHONE":"8137458524","NUM_GROUPS":"2","TOTAL_SUBJECTS":"9"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"WT1",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"NEG_WT1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"WT2",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"NEG_WT2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"WT3",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"NEG_WT3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"WT4",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"NEG_WT4.raw"}
},
{
"Subject ID":"-",
"Sample ID":"WT5",
"Factors":{"Genotype":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"NEG_WT5.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sirt2KO1",
"Factors":{"Genotype":"Sirt2 Knockout"},
"Additional sample data":{"RAW_FILE_NAME":"NEG_Sirt2KO1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sirt2KO2",
"Factors":{"Genotype":"Sirt2 Knockout"},
"Additional sample data":{"RAW_FILE_NAME":"NEG_Sirt2KO2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sirt2KO3",
"Factors":{"Genotype":"Sirt2 Knockout"},
"Additional sample data":{"RAW_FILE_NAME":"NEG_Sirt2KO3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sirt2KO4",
"Factors":{"Genotype":"Sirt2 Knockout"},
"Additional sample data":{"RAW_FILE_NAME":"NEG_Sirt2KO4.raw"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"CD8+ purified T cells from WT (n = 5) and Sirt2 KO (n = 4) mouse spleens were stimulated with plate-coated anti-CD3 (5 μg/ml, BXCELL) for 72h, followed by extensive washing of the cell pellets with PBS. All processes are carried out on ice. An aliquot (1 mL) of 80% MeOH extraction solvent is added to the sample (with Internal Standards spiked in) for protein precipitation. The 80% MeOH extraction solvent is is precooled to -80C for at least one hour. After addition of the extraction solvent, the samples are then placed in the -80C freezer for 30 minutes. After incubation, the samples are immediately centrifuged at 18,800 × g (Microfuge 22R, Beckman Coulter) at 4 C for 10 minutes. After centrifugation, the supernatant is transferred to new a microcentrifuge tube (Eppendorf brand) for drying by vacuum concentration. The dried metabolites are re-dissolved in 80% MeOH. The precipitated protein pellet is resolubilized for Bradford assays to measure the protein concentration as a quality control for the sample loading.","SAMPLE_TYPE":"Spleen","COLLECTION_METHOD":"T-cell isolation","COLLECTION_LOCATION":"Moffitt Cancer Center","COLLECTION_FREQUENCY":"1 time","VOLUMEORAMOUNT_COLLECTED":"2,000,000 cells","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"CD8+ purified T cells from WT (n = 5) and Sirt2 KO (n = 4) mouse spleens were stimulated with plate-coated anti-CD3 (5 μg/ml, BXCELL) for 72h, followed by extensive washing of the cell pellets with PBS.","TREATMENT":"anti-CD3 stimulation","TREATMENT_COMPOUND":"antibody","TREATMENT_ROUTE":"Cells placed on coated plate","TREATMENT_DOSEDURATION":"72 hours"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"All processes are carried out on ice. An aliquot (1 mL) of 80% MeOH extraction solvent is added to the sample (with Internal Standards spiked in) for protein precipitation. The 80% MeOH extraction solvent is precooled to -80C for at least one hour. After addition of the extraction solvent, the samples are then placed in the -80 oC freezer for 30 minutes. After incubation, the samples are immediately centrifuged at 18,800 × g (Microfuge 22R, Beckman Coulter) at 4 oC for 10 minutes. After centrifugation, the supernatant is transferred to new a microcentrifuge tube (Eppendorf brand) for drying by vacuum concentration. The dried metabolites are re-dissolved in 80% MeOH. The precipitated protein pellet is resolubilized for Bradford assays to measure the protein concentration as a quality control for the sample loading.","PROCESSING_STORAGE_CONDITIONS":"On ice","EXTRACTION_METHOD":"Methanol","EXTRACT_ENRICHMENT":"Vacuum centrifugation","SAMPLE_RESUSPENSION":"80% MeOH","SAMPLE_DERIVATIZATION":"None","SAMPLE_SPIKING":"Internal Standards added","SUBCELLULAR_LOCATION":"N/A"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"UHPLC-MS was performed using a Vanquish LC (Thermo, San Jose, CA) interfaced with a Q Exactive HF mass spectrometer (Thermo, San Jose, CA). Chromatographic separation was performed on a SeQuant ZIC-pHILIC LC column (150 × 4.6 mm, 5 µm particle size, MilliporeSigma, Burlington, MA). In order to maintain the stable column pressure and further filter the LC solvents, a SeQuant ZIC-pHILIC guard column (20 × 4.6 mm, 5 µm particle size, MilliporeSigma, Burlington, MA) is connected before the LC column. The mobile phase A was aqueous 10 mM ammonium carbonate and 0.05% ammonium hydroxide, and the mobile phase B was 100% acetonitrile. The total running time is 20 minutes. The column temperature was set to 30°C, and the injection volume is 2 µL.","CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Vanquish","COLUMN_NAME":"SeQuant ZIC-pHILIC","FLOW_GRADIENT":"80% B to 20%B over 13 minutes","FLOW_RATE":"0.25 ml/min","COLUMN_TEMPERATURE":"30","SOLVENT_A":"aqueous 10 mM ammonium carbonate and 0.05% ammonium hydroxide","SOLVENT_B":"100% acetonitrile","INJECTION_TEMPERATURE":"5","INTERNAL_STANDARD":"D-Glucose (2,3,4,5,6-13C5), D-Glucose-6-phosphate (U-13C6), D-Fructose-1, 6-bisphosphate (U-13C6), L-Serine (13C3), Glycine (1,2-13C2), L-Cysteine (3,3-D2), Phosphoenol Pyruvate (2,3-13C2), Lactate (3,3,3-D3), Pyruvate (D3), Acetyl-1,2-13C2 CoA, Citric Acid (2,2,4,4-D4), Alpha-Ketoglutaric Acid (1,2,3,4-13C4), Succinic Acid (D4), Fumaric Acid (D4), DL-Malic Acid (2,3,3-D3), D-Fructose-6-phosphate (U-13C6) are all from Cambridge Isotope Labs.","ANALYTICAL_TIME":"20"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Proteomics and Metabolomics Core","OPERATOR_NAME":"Min Liu, PhD","DETECTOR_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF hybrid Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Full MS is performed in positive and negative mode separately, and the mass scan range is 60 to 900 m/z. In addition to MS data acquisition 1, parallel reaction monitoring (PRM) data are acquired for the important intermediates involved in Glycolysis and the TCA cycle."},

"MS_METABOLITE_DATA":{
"Units":"Peak Intensity",

"Data":[{"Metabolite":"TARTARIC ACID","WT1":"6838.872437","WT2":"4807.664427","WT3":"1025.352317","WT4":"437.7923459","WT5":"699.5675292","Sirt2KO1":"613.9504644","Sirt2KO2":"1162.429288","Sirt2KO3":"1433.05513","Sirt2KO4":"1035.352317"},{"Metabolite":"MEVALONIC ACID","WT1":"1805.512889","WT2":"1295.043617","WT3":"963.0731534","WT4":"298.1562544","WT5":"0","Sirt2KO1":"1223.701868","Sirt2KO2":"1875.560751","Sirt2KO3":"3779.71934","Sirt2KO4":"3528.803983"},{"Metabolite":"DIHYDROOROTATE","WT1":"1691.999142","WT2":"1380.338525","WT3":"2513.59548","WT4":"719.1862276","WT5":"755.1794284","Sirt2KO1":"3890.610374","Sirt2KO2":"6973.80267","Sirt2KO3":"4072.378647","Sirt2KO4":"2343.999277"},{"Metabolite":"LACTATE","WT1":"9661124.448","WT2":"10292954.14","WT3":"8244356.922","WT4":"15017850.34","WT5":"14436443.08","Sirt2KO1":"3339376.77","Sirt2KO2":"6544655.224","Sirt2KO3":"8872995.791","Sirt2KO4":"7130026.497"},{"Metabolite":"MALATE","WT1":"2117936.462","WT2":"995420.1794","WT3":"1672063.958","WT4":"469673.8043","WT5":"489527.9891","Sirt2KO1":"2107087.188","Sirt2KO2":"2978269.157","Sirt2KO3":"4691222.465","Sirt2KO4":"5037564.515"},{"Metabolite":"10-HYDROXYDECANOATE","WT1":"5494.750113","WT2":"5867.771861","WT3":"8824.889205","WT4":"4357.573676","WT5":"5544.091677","Sirt2KO1":"2284.702437","Sirt2KO2":"4993.72795","Sirt2KO3":"11743.77358","Sirt2KO4":"7814.246915"},{"Metabolite":"2'-DEOXYGUANOSINE 5'-MONOPHOSPHATE","WT1":"359.9636521","WT2":"143.5819152","WT3":"403.5456321","WT4":"1520.489749","WT5":"1794.544508","Sirt2KO1":"690.2236461","Sirt2KO2":"1365.266594","Sirt2KO3":"1570.431162","Sirt2KO4":"4571.67382"},{"Metabolite":"2-HYDROXYBUTYRIC ACID","WT1":"11408.62252","WT2":"7607.171385","WT3":"5247.92294","WT4":"7604.874296","WT5":"7165.577919","Sirt2KO1":"2855.626858","Sirt2KO2":"8799.304288","Sirt2KO3":"7647.798349","Sirt2KO4":"8433.222908"},{"Metabolite":"3-(4-HYDROXYPHENYL)LACTATE","WT1":"16031.73265","WT2":"30034.00657","WT3":"15045.8255","WT4":"32388.15023","WT5":"35720.56015","Sirt2KO1":"9258.640733","Sirt2KO2":"14631.21827","Sirt2KO3":"17887.36052","Sirt2KO4":"15384.31899"},{"Metabolite":"3-(4-HYDROXYPHENYL)PYRUVATE","WT1":"925.5932","WT2":"658.2217804","WT3":"606.4153054","WT4":"1264.165095","WT5":"1156.19443","Sirt2KO1":"434.5809821","Sirt2KO2":"620.7932729","Sirt2KO3":"807.6139446","Sirt2KO4":"858.2313741"},{"Metabolite":"3-HYDROXY-3-METHYLGLUTARATE","WT1":"36597.93012","WT2":"40640.67633","WT3":"26140.7716","WT4":"47640.42019","WT5":"49798.95398","Sirt2KO1":"15641.21567","Sirt2KO2":"22282.47205","Sirt2KO3":"29514.47524","Sirt2KO4":"23560.68133"},{"Metabolite":"3-HYDROXYBUTANOIC ACID","WT1":"2229.042427","WT2":"2217.768533","WT3":"1756.521839","WT4":"2557.680097","WT5":"2631.534283","Sirt2KO1":"1056.688994","Sirt2KO2":"2203.514104","Sirt2KO3":"2269.30513","Sirt2KO4":"2566.189981"},{"Metabolite":"METHYL-2-OXOVALERIC ACID","WT1":"640594.3383","WT2":"467525.7066","WT3":"303638.1052","WT4":"527017.5438","WT5":"452040.9224","Sirt2KO1":"102866.4186","Sirt2KO2":"193018.8029","Sirt2KO3":"347985.269","Sirt2KO4":"293010.4335"},{"Metabolite":"3-METHYLADENINE","WT1":"5882.071864","WT2":"8257.54204","WT3":"6952.890125","WT4":"7857.786645","WT5":"7531.648997","Sirt2KO1":"3970.905728","Sirt2KO2":"6015.252436","Sirt2KO3":"11033.66863","Sirt2KO4":"16451.82001"},{"Metabolite":"ADIPIC ACID","WT1":"0","WT2":"1618.030409","WT3":"3343.738636","WT4":"0","WT5":"0","Sirt2KO1":"1901.580283","Sirt2KO2":"2421.443127","Sirt2KO3":"0","Sirt2KO4":"4716.309013"},{"Metabolite":"3-SULFINO-L-ALANINE","WT1":"1563.598446","WT2":"1861.711918","WT3":"927.1233132","WT4":"1796.702022","WT5":"1466.960282","Sirt2KO1":"763.7764572","Sirt2KO2":"1345.790089","Sirt2KO3":"2217.331221","Sirt2KO4":"2585.426167"},{"Metabolite":"4-ACETAMIDOBUTANOATE","WT1":"2861.178207","WT2":"2836.456523","WT3":"1960.361683","WT4":"3267.950954","WT5":"3083.590103","Sirt2KO1":"695.5275512","Sirt2KO2":"1255.299194","Sirt2KO3":"1816.345224","Sirt2KO4":"1786.401053"},{"Metabolite":"4-AMINOBUTANOATE","WT1":"40245.69406","WT2":"24175.54933","WT3":"32423.92222","WT4":"14021.24977","WT5":"12078.52284","Sirt2KO1":"59445.85427","Sirt2KO2":"48421.18847","Sirt2KO3":"90161.96404","Sirt2KO4":"135602.3605"},{"Metabolite":"4-HYDROXY-2-QUINOLINECARBOXYLIC ACID","WT1":"1859.85286","WT2":"2026.73447","WT3":"208.8646795","WT4":"427.5350139","WT5":"366.3202549","Sirt2KO1":"263.0887827","Sirt2KO2":"1535.683538","Sirt2KO3":"1243.646521","Sirt2KO4":"543.06079"},{"Metabolite":"4-PYRIDOXATE","WT1":"14652.60943","WT2":"10626.62206","WT3":"6465.316761","WT4":"14604.01908","WT5":"11508.707","Sirt2KO1":"2556.823549","Sirt2KO2":"3624.587515","Sirt2KO3":"5950.770637","Sirt2KO4":"5497.845024"},{"Metabolite":"5'-CMP","WT1":"1196.582102","WT2":"1199.094293","WT3":"652.9454457","WT4":"1784.236148","WT5":"1965.160857","Sirt2KO1":"471.3784202","Sirt2KO2":"890.3718066","Sirt2KO3":"3753.893868","Sirt2KO4":"3154.532591"},{"Metabolite":"5'-METHYLTHIOADENOSINE","WT1":"128.3371467","WT2":"210.6852141","WT3":"123.7328547","WT4":"276.0378034","WT5":"165.5782714","Sirt2KO1":"114.9386203","Sirt2KO2":"145.1662176","Sirt2KO3":"491.5014004","Sirt2KO4":"508.3250905"},{"Metabolite":"5-OXO-PROLINE","WT1":"1776392.514","WT2":"1645990.682","WT3":"1367133.181","WT4":"2093570.218","WT5":"2018492.659","Sirt2KO1":"797190.718","Sirt2KO2":"1106676.878","Sirt2KO3":"1730058.7","Sirt2KO4":"1633637.018"},{"Metabolite":"ADENOSINE 5'-DIPHOSPHATE","WT1":"10967.98405","WT2":"8399.180653","WT3":"6861.648914","WT4":"20551.99884","WT5":"29097.26255","Sirt2KO1":"14560.64757","Sirt2KO2":"16798.69627","Sirt2KO3":"48952.2691","Sirt2KO4":"76010.82994"},{"Metabolite":"ADENOSINE 5'-DIPHOSPHORIBOSE","WT1":"1408.525536","WT2":"2177.919749","WT3":"3190.097186","WT4":"3563.991069","WT5":"3673.15554","Sirt2KO1":"1184.398017","Sirt2KO2":"1016.711812","Sirt2KO3":"3782.794571","Sirt2KO4":"6247.435622"},{"Metabolite":"ADENOSINE 5'-MONOPHOSPHATE","WT1":"14340.99141","WT2":"8193.143478","WT3":"9467.176096","WT4":"7339.781392","WT5":"11470.51799","Sirt2KO1":"7472.117331","Sirt2KO2":"15166.06204","Sirt2KO3":"115486.4772","Sirt2KO4":"104748.5538"},{"Metabolite":"ALLANTOIN","WT1":"159193.4304","WT2":"184340.5397","WT3":"95337.05958","WT4":"157215.3772","WT5":"182899.814","Sirt2KO1":"60210.89584","Sirt2KO2":"83030.56204","Sirt2KO3":"136091.3296","Sirt2KO4":"143033.801"},{"Metabolite":"ALPHA-D-GALACTOSE 1-PHOSPHATE;D-MANNOSE 6-PHOSPHATE;D-FRUCTOSE 6-PHOSPHATE;ALPHA-D-GLUCOSE 1-PHOSPHATE","WT1":"24396.80312","WT2":"28034.24181","WT3":"26209.59797","WT4":"28160.10708","WT5":"32774.32533","Sirt2KO1":"18100.39611","Sirt2KO2":"42462.87076","Sirt2KO3":"57435.24943","Sirt2KO4":"58460.00877"},{"Metabolite":"ALPHA-D-GLUCOSE","WT1":"17042.11691","WT2":"15071.05357","WT3":"8696.439641","WT4":"13216.77429","WT5":"12554.27962","Sirt2KO1":"4286.180936","Sirt2KO2":"2290.769275","Sirt2KO3":"3960.059257","Sirt2KO4":"5092.668656"},{"Metabolite":"ALPHA-KETOGLUTARIC ACID","WT1":"389946.9306","WT2":"111339.0786","WT3":"308947.0107","WT4":"33747.53027","WT5":"34229.54459","Sirt2KO1":"160128.3654","Sirt2KO2":"237094.9226","Sirt2KO3":"587157.1197","Sirt2KO4":"261584.1911"},{"Metabolite":"13C4-ALPHA-KETOGLUTARIC ACID","WT1":"432430.0647","WT2":"598362.054","WT3":"441072.1901","WT4":"755209.3183","WT5":"804115.4361","Sirt2KO1":"367144.3356","Sirt2KO2":"611027.2981","Sirt2KO3":"803554.6027","Sirt2KO4":"1448890.626"},{"Metabolite":"ARABINOSE;D-XYLOSE;D-(-)-ARABINOSE","WT1":"5211.444607","WT2":"8895.407791","WT3":"4198.409091","WT4":"6858.288724","WT5":"8363.552672","Sirt2KO1":"3520.891502","Sirt2KO2":"4746.526004","Sirt2KO3":"3870.581958","Sirt2KO4":"2902.040638"},{"Metabolite":"ARACHIDIC ACID","WT1":"24035.88248","WT2":"29028.07591","WT3":"26244.62666","WT4":"22678.45936","WT5":"31703.42739","Sirt2KO1":"15157.8201","Sirt2KO2":"35377.64433","Sirt2KO3":"40011.02318","Sirt2KO4":"42551.03625"},{"Metabolite":"AZELAIC ACID","WT1":"2845.111664","WT2":"3883.810358","WT3":"1673.816584","WT4":"2406.851331","WT5":"1313.401701","Sirt2KO1":"910.1400678","Sirt2KO2":"7752.471737","Sirt2KO3":"2228.954009","Sirt2KO4":"2972.336709"},{"Metabolite":"BETAINE;L-NORVALINE","WT1":"4984.333258","WT2":"4622.975406","WT3":"3862.549361","WT4":"5354.790718","WT5":"5115.806972","Sirt2KO1":"61660.06763","Sirt2KO2":"27229.43196","Sirt2KO3":"8258.53002","Sirt2KO4":"9213.091317"},{"Metabolite":"BETA-NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE","WT1":"535.2936386","WT2":"607.4725862","WT3":"466.7887962","WT4":"525.1372971","WT5":"537.795944","Sirt2KO1":"472.2818665","Sirt2KO2":"779.8969701","Sirt2KO3":"1222.572302","Sirt2KO4":"1308.714458"},{"Metabolite":"CAFFEATE","WT1":"1317.273063","WT2":"1292.154971","WT3":"487.4717685","WT4":"379.8380107","WT5":"331.940648","Sirt2KO1":"382.9700837","Sirt2KO2":"898.7181241","Sirt2KO3":"737.2092423","Sirt2KO4":"887.4669729"},{"Metabolite":"CAPRYLIC ACID","WT1":"95009.61862","WT2":"80753.32992","WT3":"94856.89789","WT4":"80769.03856","WT5":"92235.98684","Sirt2KO1":"60314.07457","Sirt2KO2":"108094.0304","Sirt2KO3":"140857.4412","Sirt2KO4":"211646.626"},{"Metabolite":"CARNOSINE","WT1":"11467.82078","WT2":"10653.31928","WT3":"7126.992688","WT4":"14856.2289","WT5":"16002.57902","Sirt2KO1":"6752.736892","Sirt2KO2":"7890.524349","Sirt2KO3":"10280.74482","Sirt2KO4":"13475.39968"},{"Metabolite":"CITRACONIC ACID","WT1":"8068.256431","WT2":"7902.581278","WT3":"3396.826349","WT4":"2782.521889","WT5":"2563.361103","Sirt2KO1":"3729.753888","Sirt2KO2":"11333.74924","Sirt2KO3":"9128.740566","Sirt2KO4":"8432.381974"},{"Metabolite":"CITRATE;ISOCITRIC ACID","WT1":"352485.3791","WT2":"638156.7265","WT3":"325684.5","WT4":"592127.426","WT5":"534496.4128","Sirt2KO1":"101315.7924","Sirt2KO2":"295672.3848","Sirt2KO3":"317192.3073","Sirt2KO4":"179236.6688"},{"Metabolite":"D4-CITRIC 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