{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001412","ANALYSIS_ID":"AN002362","VERSION":"1","CREATED_ON":"June 26, 2020, 5:27 pm"},

"PROJECT":{"PROJECT_TITLE":"Metabolomics study in Plasma of Obese Patients with Neuropathy Identifies Potential Metabolomics Signatures","PROJECT_SUMMARY":"The goal of this study was to characterize biochemical profiles observed in human plasma samples originating from an obese cohort stratified by a diagnosis of neuropathy as well as a cohort of lean control subjects without a clinical manifestation of neuropathy.","INSTITUTE":"University of Michigan","LAST_NAME":"Feldman","FIRST_NAME":"Eva","ADDRESS":"5017 AATBSRB, 109 Zina Pitcher Place Ann Arbor, MI 48109-2200","EMAIL":"efeldman@med.umich.edu","PHONE":"734-763-7274"},

"STUDY":{"STUDY_TITLE":"Metabolomics study in Plasma of Obese Patients with Neuropathy Identifies Potential Metabolomics Signatures","STUDY_SUMMARY":"The goal of this study was to characterize biochemical profiles observed in human plasma samples originating from an obese cohort stratified by a diagnosis of neuropathy as well as a cohort of lean control subjects without a clinical manifestation of neuropathy.","INSTITUTE":"University of Michigan","LAST_NAME":"Feldman","FIRST_NAME":"Eva","ADDRESS":"5017 AATBSRB, 109 Zina Pitcher Place Ann Arbor, MI 48109-2200","EMAIL":"efeldman@med.umich.edu","PHONE":"734-763-7274"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"MICH-01525",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01526",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01527",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01528",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01529",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01530",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01531",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01532",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01533",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01534",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01535",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01536",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01537",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01538",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01539",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01540",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01541",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01542",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01543",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01544",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01545",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01546",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01547",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01548",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01549",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01550",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01551",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01552",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01553",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01554",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01555",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01556",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01557",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01558",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01559",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01560",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01561",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01562",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01563",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01564",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01565",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01566",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01567",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01568",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01569",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01570",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01571",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01572",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01573",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01574",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01575",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01576",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01577",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01578",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01579",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01580",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01581",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01582",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01583",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01584",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01585",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01586",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01587",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01588",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01589",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01590",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01591",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01592",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01593",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01594",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01595",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01596",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01597",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01598",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01599",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01600",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01601",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01602",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01603",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01604",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01605",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01606",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01607",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01608",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01609",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01610",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01611",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01612",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01613",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01614",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01615",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01616",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01617",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01618",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01619",
"Factors":{"Group":"Lean"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01620",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01621",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01622",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01623",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01624",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01625",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01626",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01627",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01628",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01629",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01630",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01631",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01632",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01633",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01634",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01635",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01636",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01637",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01638",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01639",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01640",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01641",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01642",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01643",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01644",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01645",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01646",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01647",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01648",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01649",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01650",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01651",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01652",
"Factors":{"Group":"Obese non neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01653",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01654",
"Factors":{"Group":"Obese neuropathy"}
},
{
"Subject ID":"-",
"Sample ID":"MICH-01655",
"Factors":{"Group":"Obese neuropathy"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Plasma was collected in purple EDTA tubes with 10 mL of buffy coat, inverted 10 times, incubated for 30-90 minutes at room temperature, and centrifuged at 3000 rpm for 10 minutes. The plasma supernatant was collected by aspirating plasma to approximately 5 mm above the buffy coat and plasma samples were stored in 0.5 mL aliquots at -80C prior to metabolomics analysis.","SAMPLE_TYPE":"Blood (plasma)"},

"TREATMENT":{"TREATMENT_SUMMARY":"None"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Low pH polar","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity BEH C18 (100 x 2mm, 1.7um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Proprietary analytical software for integration and peak picking"},

"MS_METABOLITE_DATA":{
"Units":"peak area",

"Data":[{"Metabolite":"(12 or 13)-methylmyristate (a15:0 or 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}