{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001433","ANALYSIS_ID":"AN002395","VERSION":"1","CREATED_ON":"July 27, 2020, 11:33 am"},

"PROJECT":{"PROJECT_TITLE":"Evidence for proline utilisation by oral bacterial biofilms grown in saliva","PROJECT_TYPE":"Research study","PROJECT_SUMMARY":"Within the mouth bacteria are starved of saccharides as their main nutrient source between meals and it is unclear what drives their metabolism. Previously oral in vitro biofilms grown in saliva have shown proteolytic degradation of salivary proteins and increased extracellular proline. Although arginine and glucose have been shown before to have an effect on oral biofilm growth and activity, there is limited evidence for proline. Nuclear magnetic resonance (NMR) spectroscopy was used to identify extracellular metabolites produced by bacteria in oral biofilms grown on hydroxyapatite discs. Biofilms were inoculated with whole mouth saliva and then grown for 7 days using sterilised whole mouth saliva supplemented with proline, arginine and glucose as a growth-medium. Overall proline had a beneficial effect on biofilm growth – with significantly fewer dead bacteria present by biomass and surface area of the biofilms (p <0.05). Where arginine and glucose significantly increased and decreased pH, respectively, the pH of proline supplemented biofilms remained neutral at pH 7.3-7.5. SDS-polyacrylamide gel electrophoresis of the spent saliva from proline and arginine supplemented biofilms showed inhibition of salivary protein degradation of immature biofilms. NMR analysis of the spent saliva revealed that proline supplemented biofilms were metabolically similar to unsupplemented biofilms, but these biofilms actively metabolised proline to 5-aminopentanoate, butyrate and propionate, and actively utilised glycine. This study shows that in a nutrient limited environment, proline has a beneficial effect on in vitro oral biofilms grown from a saliva inoculum.","INSTITUTE":"King's College London","DEPARTMENT":"Centre for Host Microbiome Interactions","LAST_NAME":"Cleaver","FIRST_NAME":"Leanne","ADDRESS":"Floor 17, Tower Wing, Guy's Hospital, Great Maze Pond, London, London, SE1 9RT, United Kingdom","EMAIL":"leanne.cleaver@kcl.ac.uk","PHONE":"07464626438","FUNDING_SOURCE":"This work was funded by a BBSRC Lido-associated PhD studentship for LC in association with Colgate-Palmolive, USA."},

"STUDY":{"STUDY_TITLE":"Evidence for proline utilisation by oral bacterial biofilms grown in saliva","STUDY_TYPE":"Research study","STUDY_SUMMARY":"Within the mouth bacteria are starved of saccharides as their main nutrient source between meals and it is unclear what drives their metabolism. Previously oral in vitro biofilms grown in saliva have shown proteolytic degradation of salivary proteins and increased extracellular proline. Although arginine and glucose have been shown before to have an effect on oral biofilm growth and activity, there is limited evidence for proline. Nuclear magnetic resonance (NMR) spectroscopy was used to identify extracellular metabolites produced by bacteria in oral biofilms grown on hydroxyapatite discs. Biofilms were inoculated with whole mouth saliva and then grown for 7 days using sterilised whole mouth saliva supplemented with proline, arginine and glucose as a growth-medium. Overall proline had a beneficial effect on biofilm growth – with significantly fewer dead bacteria present by biomass and surface area of the biofilms (p <0.05). Where arginine and glucose significantly increased and decreased pH, respectively, the pH of proline supplemented biofilms remained neutral at pH 7.3-7.5. SDS-polyacrylamide gel electrophoresis of the spent saliva from proline and arginine supplemented biofilms showed inhibition of salivary protein degradation of immature biofilms. NMR analysis of the spent saliva revealed that proline supplemented biofilms were metabolically similar to unsupplemented biofilms, but these biofilms actively metabolised proline to 5-aminopentanoate, butyrate and propionate, and actively utilised glycine. This study shows that in a nutrient limited environment, proline has a beneficial effect on in vitro oral biofilms grown from a saliva inoculum.","INSTITUTE":"King's College London","DEPARTMENT":"Centre for Host Microbiome Interactions","LAST_NAME":"Cleaver","FIRST_NAME":"Leanne","ADDRESS":"Floor 17, Tower Wing, Guy's Hospital, Great Maze Pond","EMAIL":"leanne.cleaver@kcl.ac.uk","PHONE":"07464626438","NUM_GROUPS":"11"},

"SUBJECT":{"SUBJECT_TYPE":"Bacteria","SUBJECT_SPECIES":"Multi-species non-defined biofilm consortium"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"Sterile Saliva",
"Sample ID":"sample1",
"Factors":{"Treatment":"Growth Medium"},
"Additional sample data":{"RAW_FILE_NAME":"sample1.raw"}
},
{
"Subject ID":"P10 1 D4",
"Sample ID":"sample2",
"Factors":{"Treatment":"Proline 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample2.raw"}
},
{
"Subject ID":"P10 2 D4",
"Sample ID":"sample3",
"Factors":{"Treatment":"Proline 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample3.raw"}
},
{
"Subject ID":"P10 3 D4",
"Sample ID":"sample4",
"Factors":{"Treatment":"Proline 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample4.raw"}
},
{
"Subject ID":"A10 1 D4",
"Sample ID":"sample5",
"Factors":{"Treatment":"Arginine 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample5.raw"}
},
{
"Subject ID":"A10 2 D4",
"Sample ID":"sample6",
"Factors":{"Treatment":"Arginine 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample6.raw"}
},
{
"Subject ID":"A10 3 D4",
"Sample ID":"sample7",
"Factors":{"Treatment":"Arginine 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample7.raw"}
},
{
"Subject ID":"G10 1 D4",
"Sample ID":"sample8",
"Factors":{"Treatment":"Glucose 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample8.raw"}
},
{
"Subject ID":"G10 2 D4",
"Sample ID":"sample9",
"Factors":{"Treatment":"Glucose 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample9.raw"}
},
{
"Subject ID":"G10 3 D4",
"Sample ID":"sample10",
"Factors":{"Treatment":"Glucose 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample10.raw"}
},
{
"Subject ID":"P25 1 D4",
"Sample ID":"sample11",
"Factors":{"Treatment":"Proline 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample11.raw"}
},
{
"Subject ID":"P25 2 D4",
"Sample ID":"sample12",
"Factors":{"Treatment":"Proline 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample12.raw"}
},
{
"Subject ID":"P25 3 D4",
"Sample ID":"sample13",
"Factors":{"Treatment":"Proline 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample13.raw"}
},
{
"Subject ID":"A25 1 D4",
"Sample ID":"sample14",
"Factors":{"Treatment":"Arginine 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample14.raw"}
},
{
"Subject ID":"A25 2 D4",
"Sample ID":"sample15",
"Factors":{"Treatment":"Arginine 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample15.raw"}
},
{
"Subject ID":"A25 3 D4",
"Sample ID":"sample16",
"Factors":{"Treatment":"Arginine 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample16.raw"}
},
{
"Subject ID":"G25 1 D4",
"Sample ID":"sample17",
"Factors":{"Treatment":"Glucose 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample17.raw"}
},
{
"Subject ID":"G25 2 D4",
"Sample ID":"sample18",
"Factors":{"Treatment":"Glucose 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample18.raw"}
},
{
"Subject ID":"G25 3 D4",
"Sample ID":"sample19",
"Factors":{"Treatment":"Glucose 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample19.raw"}
},
{
"Subject ID":"P50 1 D4",
"Sample ID":"sample20",
"Factors":{"Treatment":"Proline 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample20.raw"}
},
{
"Subject ID":"P50 2 D4",
"Sample ID":"sample21",
"Factors":{"Treatment":"Proline 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample21.raw"}
},
{
"Subject ID":"P50 3 D4",
"Sample ID":"sample22",
"Factors":{"Treatment":"Proline 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample22.raw"}
},
{
"Subject ID":"A50 1 D4",
"Sample ID":"sample23",
"Factors":{"Treatment":"Arginine 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample23.raw"}
},
{
"Subject ID":"A50 2 D4",
"Sample ID":"sample24",
"Factors":{"Treatment":"Arginine 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample24.raw"}
},
{
"Subject ID":"A50 3 D4",
"Sample ID":"sample25",
"Factors":{"Treatment":"Arginine 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample25.raw"}
},
{
"Subject ID":"G50 1 D4",
"Sample ID":"sample26",
"Factors":{"Treatment":"Glucose 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample26.raw"}
},
{
"Subject ID":"G50 2 D4",
"Sample ID":"sample27",
"Factors":{"Treatment":"Glucose 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample27.raw"}
},
{
"Subject ID":"G50 3 D4",
"Sample ID":"sample28",
"Factors":{"Treatment":"Glucose 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample28.raw"}
},
{
"Subject ID":"Unsupplemented Control 1 D4",
"Sample ID":"sample29",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample29.raw"}
},
{
"Subject ID":"Unsupplemented Control 2 D4",
"Sample ID":"sample30",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample30.raw"}
},
{
"Subject ID":"Unsupplemented Control 3 D4",
"Sample ID":"sample31",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample31.raw"}
},
{
"Subject ID":"Negative Control 1 D4",
"Sample ID":"sample32",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample32.raw"}
},
{
"Subject ID":"Negative Control 2 D4",
"Sample ID":"sample33",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample33.raw"}
},
{
"Subject ID":"Negative Control 3 D4",
"Sample ID":"sample34",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample34.raw"}
},
{
"Subject ID":"P10 1 D7",
"Sample ID":"sample35",
"Factors":{"Treatment":"Proline 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample35.raw"}
},
{
"Subject ID":"P10 2 D7",
"Sample ID":"sample36",
"Factors":{"Treatment":"Proline 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample36.raw"}
},
{
"Subject ID":"P10 3 D7",
"Sample ID":"sample37",
"Factors":{"Treatment":"Proline 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample37.raw"}
},
{
"Subject ID":"A10 1 D7",
"Sample ID":"sample38",
"Factors":{"Treatment":"Arginine 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample38.raw"}
},
{
"Subject ID":"A10 2 D7",
"Sample ID":"sample39",
"Factors":{"Treatment":"Arginine 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample39.raw"}
},
{
"Subject ID":"A10 3 D7",
"Sample ID":"sample40",
"Factors":{"Treatment":"Arginine 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample40.raw"}
},
{
"Subject ID":"G10 1 D7",
"Sample ID":"sample41",
"Factors":{"Treatment":"Glucose 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample41.raw"}
},
{
"Subject ID":"G10 2 D7",
"Sample ID":"sample42",
"Factors":{"Treatment":"Glucose 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample42.raw"}
},
{
"Subject ID":"G10 3 D7",
"Sample ID":"sample43",
"Factors":{"Treatment":"Glucose 10mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample43.raw"}
},
{
"Subject ID":"P25 1 D7",
"Sample ID":"sample44",
"Factors":{"Treatment":"Proline 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample44.raw"}
},
{
"Subject ID":"P25 2 D7",
"Sample ID":"sample45",
"Factors":{"Treatment":"Proline 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample45.raw"}
},
{
"Subject ID":"P25 3 D7",
"Sample ID":"sample46",
"Factors":{"Treatment":"Proline 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample46.raw"}
},
{
"Subject ID":"A25 1 D7",
"Sample ID":"sample47",
"Factors":{"Treatment":"Arginine 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample47.raw"}
},
{
"Subject ID":"A25 2 D7",
"Sample ID":"sample48",
"Factors":{"Treatment":"Arginine 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample48.raw"}
},
{
"Subject ID":"A25 3 D7",
"Sample ID":"sample49",
"Factors":{"Treatment":"Arginine 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample49.raw"}
},
{
"Subject ID":"G25 1 D7",
"Sample ID":"sample50",
"Factors":{"Treatment":"Glucose 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample50.raw"}
},
{
"Subject ID":"G25 2 D7",
"Sample ID":"sample51",
"Factors":{"Treatment":"Glucose 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample51.raw"}
},
{
"Subject ID":"G25 3 D7",
"Sample ID":"sample52",
"Factors":{"Treatment":"Glucose 25mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample52.raw"}
},
{
"Subject ID":"P50 1 D7",
"Sample ID":"sample53",
"Factors":{"Treatment":"Proline 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample53.raw"}
},
{
"Subject ID":"P50 2 D7",
"Sample ID":"sample54",
"Factors":{"Treatment":"Proline 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample54.raw"}
},
{
"Subject ID":"P50 3 D7",
"Sample ID":"sample55",
"Factors":{"Treatment":"Proline 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample55.raw"}
},
{
"Subject ID":"A50 1 D7",
"Sample ID":"sample56",
"Factors":{"Treatment":"Arginine 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample56.raw"}
},
{
"Subject ID":"A50 2 D7",
"Sample ID":"sample57",
"Factors":{"Treatment":"Arginine 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample57.raw"}
},
{
"Subject ID":"A50 3 D7",
"Sample ID":"sample58",
"Factors":{"Treatment":"Arginine 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample58.raw"}
},
{
"Subject ID":"G50 1 D7",
"Sample ID":"sample59",
"Factors":{"Treatment":"Glucose 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample59.raw"}
},
{
"Subject ID":"G50 2 D7",
"Sample ID":"sample60",
"Factors":{"Treatment":"Glucose 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample60.raw"}
},
{
"Subject ID":"G50 3 D7",
"Sample ID":"sample61",
"Factors":{"Treatment":"Glucose 50mM"},
"Additional sample data":{"RAW_FILE_NAME":"sample61.raw"}
},
{
"Subject ID":"Unsupplemented Control 1 D7",
"Sample ID":"sample62",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample62.raw"}
},
{
"Subject ID":"Unsupplemented Control 2 D7",
"Sample ID":"sample63",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample63.raw"}
},
{
"Subject ID":"Unsupplemented Control 3 D7",
"Sample ID":"sample64",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample64.raw"}
},
{
"Subject ID":"Negative Control 1 D7",
"Sample ID":"sample65",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample65.raw"}
},
{
"Subject ID":"Negative Control 2 D7",
"Sample ID":"sample66",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample66.raw"}
},
{
"Subject ID":"Negative Control 3 D7",
"Sample ID":"sample67",
"Factors":{"Treatment":"No treatment"},
"Additional sample data":{"RAW_FILE_NAME":"sample67.raw"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Saliva was processed and biofilms were grown using a previously published methodology with modifications (Cleaver, Moazzez and Carpenter, 2019). Saliva samples were centrifuged (5000g for 5 mins), and the supernatant was collected, pooled, boiled in sealed tubes for 20 minutes to sterilise, and left to cool to room temperature; hereafter referred to as sterile saliva. The loose pellet from the spun saliva samples was pooled and combined with enough sterile saliva to facilitate inoculation of the hydroxyapatite (HA) discs. Samples were vortexed vigorously to resuspend the pellet; this constituted the pooled saliva inoculum. The remaining sterile saliva was split into 11 aliquots. D-proline, L-arginine, and glucose were added to the aliquots to achieve concentrations of 10mM, 25mM and 50mM respectively for each (based on concentrations of proline obtained from previous publication (6)). One aliquot was also supplemented with 10mM carbon 13 labelled (C13) D-proline. Two aliquots were left unsupplemented to act as a positive and negative control. Six HA discs in a sterile microtitre plate were inoculated per treatment condition with 1 ml pooled saliva inoculum (negative control discs were inoculated with sterile saliva only to check for sterility) and incubated for 24 hours aerobically in a 40 L aerobic incubator (GenLab Ltd, UK) at 37°C. After this time the discs were washed three times with sterile phosphate buffered saline (PBS) and 1 ml of supplemented/unsupplemented saliva per condition was added to the discs. The discs were then incubated anaerobically in a 3.5 litre anaerobic jar with an Anaerogen anaerobic generator pack (Oxoid, UK) at 37°C for 72 hours. After this spent saliva from the biofilms was removed and stored at -20°C for further analysis, discs were washed three times with PBS, and refreshed with supplemented/unsupplemented saliva and further incubated anaerobically at 37°C for 72 hours. After this final incubation the spent saliva was removed and stored at -20°C for further analysis.","SAMPLE_TYPE":"Bacterial cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"Sterile saliva was split into 11 aliquots. D-proline, L-arginine, and glucose were added to the aliquots to achieve concentrations of 10mM, 25mM and 50mM respectively for each (based on concentrations of proline obtained from previous publication (6)). One aliquot was also supplemented with 10mM carbon 13 labelled (C13) D-proline. Two aliquots were left unsupplemented to act as a positive and negative control."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were processed for NMR using a previously published method (6). Briefly, centrifuged spent saliva supernatant and the sterile saliva sample were each mixed with TSP buffer in a 5 mm NMR tube (Bruker, Germany). The tubes were sealed and analysed at the Biomolecular Spectroscopy Centre, King’s College London, UK on a 600 MHz spectrometer (Bruker) for 1H 1D-NMR and 1H-C13 1D- and 2D-NMR. The concentration of metabolites relative to the sterile saliva baseline were collected using Chenomix NMR Suite version 8.5 (Chenomix Ltd, Canada). The 2D C13 spectra were analysed using TopSpin version 3.6.2 (Bruker) and COLMAR (30)."},

"ANALYSIS":{"ANALYSIS_TYPE":"NMR"},

"NM":{"INSTRUMENT_NAME":"Bruker","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","SPECTROMETER_FREQUENCY":"600Hz"},

"NMR_METABOLITE_DATA":{
"Units":"mM",

"Data":[{"Metabolite":"5-Aminopentanoic acid","sample1":"0","sample2":"1.5654","sample3":"1.2133","sample4":"1.497","sample5":"0.9911","sample6":"0.6478","sample7":"0.6567","sample8":"0.8604","sample9":"0.607","sample10":"0.7329","sample11":"1.4586","sample12":"1.082","sample13":"1.3326","sample14":"0.1244","sample15":"0.1105","sample16":"0.0909","sample17":"0","sample18":"0.1831","sample19":"0.171","sample20":"1.3569","sample21":"1.4923","sample22":"1.2555","sample23":"0.0926","sample24":"0.1034","sample25":"0.1","sample26":"0","sample27":"0.141","sample28":"0","sample29":"1.2992","sample30":"1.3429","sample31":"1.0774","sample32":"0.1125","sample33":"0.097","sample34":"0.0942","sample35":"0.7139","sample36":"0.479","sample37":"0.8383","sample38":"1.244","sample39":"1.0811","sample40":"1.2903","sample41":"0.3094","sample42":"0.4528","sample43":"0.4444","sample44":"0.8435","sample45":"0.993","sample46":"0.7779","sample47":"0.136","sample48":"0.0926","sample49":"0.0944","sample50":"0.0966","sample51":"0.0894","sample52":"0.3468","sample53":"0.9219","sample54":"1.0341","sample55":"0.9383","sample56":"0.0831","sample57":"0.1027","sample58":"0.0821","sample59":"0.081","sample60":"0","sample61":"0.104","sample62":"2.3716","sample63":"2.4505","sample64":"1.7254","sample65":"0.0909","sample66":"0","sample67":"0.1122"},{"Metabolite":"Propionic acid","sample1":"0.2998","sample2":"0.4734","sample3":"0.4305","sample4":"0.4649","sample5":"0.3957","sample6":"0.4064","sample7":"0.4493","sample8":"0.2783","sample9":"0.5404","sample10":"0.7805","sample11":"0.514","sample12":"0.3812","sample13":"0.4689","sample14":"0.3322","sample15":"0.3114","sample16":"0.2631","sample17":"0.1716","sample18":"0.1792","sample19":"0.1736","sample20":"0.5653","sample21":"0.5713","sample22":"0.6098","sample23":"0.2473","sample24":"0.2697","sample25":"0.2945","sample26":"0.1411","sample27":"0.1638","sample28":"0.1614","sample29":"0.3945","sample30":"0.3777","sample31":"0.365","sample32":"0.2722","sample33":"0.249","sample34":"0.218","sample35":"0.7021","sample36":"0.5379","sample37":"0.4774","sample38":"0.2986","sample39":"0.3133","sample40":"0.3461","sample41":"0.2146","sample42":"1.4394","sample43":"1.2878","sample44":"0.6322","sample45":"0.7363","sample46":"0.9528","sample47":"0.3631","sample48":"0.2198","sample49":"0.2398","sample50":"0.1895","sample51":"0.1785","sample52":"0.15","sample53":"0.6691","sample54":"0.5361","sample55":"0.6499","sample56":"0.2283","sample57":"0.2674","sample58":"0.2105","sample59":"0.1843","sample60":"0.1834","sample61":"0.2223","sample62":"0.3926","sample63":"0.4303","sample64":"0.2997","sample65":"0.2271","sample66":"0.13","sample67":"0.2255"},{"Metabolite":"Butyric acid","sample1":"0","sample2":"0.8958","sample3":"0.7544","sample4":"0.8345","sample5":"0.5692","sample6":"0.5254","sample7":"0.6559","sample8":"0.0595","sample9":"0.1175","sample10":"0.222","sample11":"0.827","sample12":"0.7416","sample13":"0.7288","sample14":"0.0752","sample15":"0.0688","sample16":"0.0505","sample17":"0.0389","sample18":"0.0428","sample19":"0.0361","sample20":"0.9427","sample21":"0.9134","sample22":"0.8441","sample23":"0.0509","sample24":"0.0558","sample25":"0.0613","sample26":"0.0256","sample27":"0.0283","sample28":"0.0284","sample29":"0.496","sample30":"0.1439","sample31":"0.324","sample32":"0.0389","sample33":"0.037","sample34":"0.0338","sample35":"1.2285","sample36":"1.1275","sample37":"0.8096","sample38":"0.4204","sample39":"0.3626","sample40":"0.366","sample41":"0.0482","sample42":"0.3053","sample43":"0.0518","sample44":"0.9916","sample45":"1.0682","sample46":"1.1235","sample47":"0.0777","sample48":"0.0442","sample49":"0.0495","sample50":"0.0359","sample51":"0.032","sample52":"0.0358","sample53":"0.9988","sample54":"0.9367","sample55":"0.8815","sample56":"0.0452","sample57":"0.0563","sample58":"0.0437","sample59":"0.0389","sample60":"0.0311","sample61":"0.0375","sample62":"0.9169","sample63":"0.8141","sample64":"0.6562","sample65":"0.0333","sample66":"0","sample67":"0.0345"},{"Metabolite":"Formic acid","sample1":"0.0985","sample2":"2.6695","sample3":"1.6217","sample4":"0.5505","sample5":"1.5973","sample6":"1.4291","sample7":"1.3268","sample8":"4.5712","sample9":"3.4475","sample10":"4.7489","sample11":"1.7439","sample12":"1.0552","sample13":"1.0519","sample14":"1.19","sample15":"1.2907","sample16":"0.9776","sample17":"0.4044","sample18":"0.7437","sample19":"0.8219","sample20":"1.3699","sample21":"1.1744","sample22":"0.68","sample23":"0.8192","sample24":"0.7415","sample25":"1.1531","sample26":"0.2516","sample27":"0.4002","sample28":"0.2569","sample29":"0.1132","sample30":"0.7764","sample31":"0.9212","sample32":"0.0845","sample33":"0.0628","sample34":"0.0783","sample35":"0.244","sample36":"0.0019","sample37":"0","sample38":"0.2049","sample39":"0.512","sample40":"1.2597","sample41":"1.7747","sample42":"4.0336","sample43":"3.1813","sample44":"0.1744","sample45":"0.0801","sample46":"0.1304","sample47":"0.8626","sample48":"0.5828","sample49":"1.0689","sample50":"0.1239","sample51":"0.055","sample52":"0.2983","sample53":"0.0068","sample54":"0.0451","sample55":"0.0335","sample56":"0.611","sample57":"0.6522","sample58":"0.5172","sample59":"0.0385","sample60":"0.5788","sample61":"0.0732","sample62":"0.7838","sample63":"0.5065","sample64":"0.6417","sample65":"0","sample66":"0.0888","sample67":"0.0784"},{"Metabolite":"Ethanol","sample1":"0","sample2":"0.2079","sample3":"0.1804","sample4":"0.1983","sample5":"0.2056","sample6":"0.2583","sample7":"0.2702","sample8":"0.4515","sample9":"0.3359","sample10":"0.4398","sample11":"0.133","sample12":"0.1103","sample13":"0.125","sample14":"0.5247","sample15":"0.4738","sample16":"0.402","sample17":"0.4251","sample18":"0.4126","sample19":"0.4171","sample20":"0.1532","sample21":"0.1484","sample22":"0.1535","sample23":"0.3958","sample24":"0.4094","sample25":"0.4352","sample26":"0.2651","sample27":"0.3242","sample28":"0.281","sample29":"0.2261","sample30":"0.2119","sample31":"0.235","sample32":"0.4181","sample33":"0.3854","sample34":"0.3166","sample35":"0.7769","sample36":"0.7835","sample37":"0.6233","sample38":"0.7268","sample39":"0.7109","sample40":"0.7454","sample41":"0.8141","sample42":"0.794","sample43":"0.8607","sample44":"0.5354","sample45":"0.663","sample46":"0.6988","sample47":"1.2266","sample48":"0.7867","sample49":"0.8576","sample50":"0.9935","sample51":"0.9409","sample52":"1.0338","sample53":"0.4825","sample54":"0.4774","sample55":"0.5208","sample56":"0.8509","sample57":"0.862","sample58":"0.757","sample59":"0.8758","sample60":"0.8921","sample61":"1.0411","sample62":"1.0385","sample63":"1.0762","sample64":"1.0609","sample65":"1.2718","sample66":"0.897","sample67":"1.1264"},{"Metabolite":"Acetic acid","sample1":"2.1818","sample2":"9.021","sample3":"7.9856","sample4":"8.7387","sample5":"6.9626","sample6":"7.2142","sample7":"8.1821","sample8":"7.4442","sample9":"6.1854","sample10":"7.9605","sample11":"8.6239","sample12":"7.3983","sample13":"7.7907","sample14":"4.492","sample15":"4.0641","sample16":"3.4935","sample17":"2.2735","sample18":"2.5761","sample19":"2.6142","sample20":"9.5797","sample21":"9.3113","sample22":"8.8818","sample23":"3.2345","sample24":"3.56","sample25":"3.9599","sample26":"1.6903","sample27":"2.2496","sample28":"1.7313","sample29":"6.377","sample30":"6.0199","sample31":"6.9844","sample32":"2.0251","sample33":"1.8528","sample34":"1.6413","sample35":"12.1875","sample36":"10.6843","sample37":"9.2826","sample38":"5.9131","sample39":"6.332","sample40":"6.9559","sample41":"4.1082","sample42":"7.6525","sample43":"6.5859","sample44":"11.194","sample45":"11.8059","sample46":"11.9926","sample47":"4.1478","sample48":"2.6911","sample49":"3.3318","sample50":"1.5863","sample51":"1.4287","sample52":"1.7548","sample53":"9.9783","sample54":"10.437","sample55":"9.5848","sample56":"2.6612","sample57":"3.2183","sample58":"2.5034","sample59":"1.4363","sample60":"1.8431","sample61":"1.6418","sample62":"10.0832","sample63":"10.0404","sample64":"8.2539","sample65":"1.7943","sample66":"1.8702","sample67":"1.7765"},{"Metabolite":"Phenylalanine","sample1":"0","sample2":"0.0589","sample3":"0.0534","sample4":"0.0573","sample5":"0.0391","sample6":"0.0451","sample7":"0.0527","sample8":"0.0526","sample9":"0.0379","sample10":"0.0446","sample11":"0.062","sample12":"0.0511","sample13":"0.0522","sample14":"0.0485","sample15":"0.0419","sample16":"0.0336","sample17":"0.0278","sample18":"0.0269","sample19":"0.0249","sample20":"0.0542","sample21":"0.0594","sample22":"0.0559","sample23":"0.0363","sample24":"0.035","sample25":"0.0391","sample26":"0.0251","sample27":"0.0286","sample28":"0.0261","sample29":"0","sample30":"0","sample31":"0.0529","sample32":"0","sample33":"0","sample34":"0","sample35":"0.0604","sample36":"0.0642","sample37":"0.0461","sample38":"0.0324","sample39":"0.0334","sample40":"0.0365","sample41":"0.0343","sample42":"0.0436","sample43":"0.0336","sample44":"0.0531","sample45":"0.0641","sample46":"0.0729","sample47":"0.0438","sample48":"0","sample49":"0.0304","sample50":"0.0331","sample51":"0.0325","sample52":"0.0331","sample53":"0.0546","sample54":"0.0521","sample55":"0.0473","sample56":"0.0258","sample57":"0.0329","sample58":"0.0268","sample59":"0.0311","sample60":"0.0304","sample61":"0.0065","sample62":"0.0476","sample63":"0.0556","sample64":"0.0318","sample65":"0","sample66":"0","sample67":"0"},{"Metabolite":"Alanine","sample1":"0.019","sample2":"0","sample3":"0","sample4":"0","sample5":"0","sample6":"0.0759","sample7":"0.1358","sample8":"0.1603","sample9":"0.1134","sample10":"0.1297","sample11":"0","sample12":"0","sample13":"0","sample14":"0.2194","sample15":"0.1774","sample16":"0.1454","sample17":"0.0804","sample18":"0.0727","sample19":"0.0656","sample20":"0","sample21":"0","sample22":"0","sample23":"0.135","sample24":"0.1469","sample25":"0.156","sample26":"0","sample27":"0.0817","sample28":"0","sample29":"0","sample30":"0","sample31":"0","sample32":"0.016","sample33":"0.0146","sample34":"0.0133","sample35":"0","sample36":"0","sample37":"0","sample38":"0.0233","sample39":"0","sample40":"0.0316","sample41":"0.1045","sample42":"0.1204","sample43":"0.0694","sample44":"0","sample45":"0","sample46":"0","sample47":"0.177","sample48":"0.1031","sample49":"0.1423","sample50":"0.0542","sample51":"0","sample52":"0.0767","sample53":"0","sample54":"0","sample55":"0","sample56":"0.0946","sample57":"0.1688","sample58":"0.114","sample59":"0","sample60":"0","sample61":"0.0098","sample62":"0","sample63":"0","sample64":"0","sample65":"0.0129","sample66":"0","sample67":"0.0146"},{"Metabolite":"Lactic acid","sample1":"0.1683","sample2":"0.0048","sample3":"0.0039","sample4":"0.0086","sample5":"0.0073","sample6":"0.0267","sample7":"0.0281","sample8":"8.6563","sample9":"5.8085","sample10":"7.1043","sample11":"0.0064","sample12":"0.0037","sample13":"0.004","sample14":"0.2784","sample15":"0.1667","sample16":"0.1613","sample17":"13.3291","sample18":"13.5382","sample19":"13.1396","sample20":"0.006","sample21":"0.0039","sample22":"0.004","sample23":"0.2619","sample24":"0.2844","sample25":"0.1352","sample26":"11.0059","sample27":"13.6119","sample28":"13.4516","sample29":"0.006","sample30":"0.0188","sample31":"0.0064","sample32":"0.1465","sample33":"0.1356","sample34":"0.1201","sample35":"0.0047","sample36":"0.0037","sample37":"0.004","sample38":"0.0155","sample39":"0.0082","sample40":"0.0164","sample41":"10.2342","sample42":"7.9777","sample43":"7.8013","sample44":"0.0074","sample45":"0.0044","sample46":"0.0046","sample47":"0.4477","sample48":"0.2255","sample49":"0.3623","sample50":"15.1106","sample51":"13.1143","sample52":"14.6809","sample53":"0.0126","sample54":"0","sample55":"0","sample56":"0.2508","sample57":"0.2595","sample58":"0.1795","sample59":"10.4028","sample60":"13.0744","sample61":"0.1516","sample62":"0.0082","sample63":"0.0057","sample64":"0.0103","sample65":"0.124","sample66":"0.1184","sample67":"0.1246"},{"Metabolite":"Putrescine","sample1":"0","sample2":"0.2748","sample3":"0.2358","sample4":"0.2553","sample5":"3.3936","sample6":"3.8634","sample7":"3.7586","sample8":"0","sample9":"0","sample10":"0","sample11":"0.2622","sample12":"0.2306","sample13":"0.2518","sample14":"5.7624","sample15":"4.7715","sample16":"4.4849","sample17":"0","sample18":"0","sample19":"0","sample20":"0.3011","sample21":"0.29","sample22":"0.2777","sample23":"3.5543","sample24":"3.7516","sample25":"4.6764","sample26":"0","sample27":"0","sample28":"0","sample29":"0.215","sample30":"0.2405","sample31":"0.2464","sample32":"0","sample33":"0","sample34":"0","sample35":"0.3457","sample36":"0.332","sample37":"0.2734","sample38":"2.6905","sample39":"2.8036","sample40":"2.4468","sample41":"0","sample42":"0","sample43":"0","sample44":"0.3223","sample45":"0.3325","sample46":"0.4096","sample47":"5.3743","sample48":"3.8358","sample49":"3.3974","sample50":"0","sample51":"0","sample52":"0","sample53":"0.2659","sample54":"0.28","sample55":"0.2412","sample56":"2.9041","sample57":"5.5422","sample58":"4.1946","sample59":"0","sample60":"0","sample61":"0","sample62":"0.2824","sample63":"0.29","sample64":"0.2145","sample65":"0","sample66":"0","sample67":"0"},{"Metabolite":"Methanol","sample1":"0.0242","sample2":"0.1019","sample3":"0.0864","sample4":"0.0926","sample5":"0.0698","sample6":"0.0872","sample7":"0.0872","sample8":"0.0689","sample9":"0.0516","sample10":"0.0684","sample11":"0.6982","sample12":"0.6049","sample13":"0.654","sample14":"0.0951","sample15":"0.0886","sample16":"0.0748","sample17":"0.0727","sample18":"0.0687","sample19":"0.0707","sample20":"1.3552","sample21":"1.2888","sample22":"1.2279","sample23":"0.0731","sample24":"0.0725","sample25":"0.0783","sample26":"0.0693","sample27":"0.0794","sample28":"0.0729","sample29":"0.0835","sample30":"0.0838","sample31":"0.087","sample32":"0.0883","sample33":"0.0892","sample34":"0.079","sample35":"0.1657","sample36":"0.1685","sample37":"0.1395","sample38":"0.1322","sample39":"0.1278","sample40":"0.1368","sample41":"0.1062","sample42":"0.1051","sample43":"0.1113","sample44":"0.8419","sample45":"0.1888","sample46":"1.0258","sample47":"0.1652","sample48":"0.1044","sample49":"0.111","sample50":"0.1209","sample51":"0.1144","sample52":"0.1253","sample53":"1.2542","sample54":"0.1902","sample55":"0.227","sample56":"0.1127","sample57":"0.1176","sample58":"0.1011","sample59":"0.1169","sample60":"0.1179","sample61":"0.1339","sample62":"0.15","sample63":"0.1519","sample64":"0.1472","sample65":"0.1636","sample66":"0.1327","sample67":"0.1426"},{"Metabolite":"Proline","sample1":"0","sample2":"6.6555","sample3":"5.58","sample4":"6.0444","sample5":"1.1481","sample6":"1.3558","sample7":"1.3206","sample8":"0","sample9":"0","sample10":"0","sample11":"14.087","sample12":"12.0003","sample13":"13.1289","sample14":"0.6823","sample15":"0.6464","sample16":"0.575","sample17":"0.3187","sample18":"0.3176","sample19":"0","sample20":"25.2884","sample21":"25.4488","sample22":"24.0924","sample23":"0.4117","sample24":"0.4181","sample25":"0.5329","sample26":"0","sample27":"0","sample28":"0","sample29":"0.1843","sample30":"0.4324","sample31":"0.8055","sample32":"0","sample33":"0","sample34":"0","sample35":"9.1519","sample36":"8.663","sample37":"5.3857","sample38":"0","sample39":"0.4241","sample40":"0","sample41":"0.5385","sample42":"0.3228","sample43":"0.5624","sample44":"17.1239","sample45":"18.2361","sample46":"19.778","sample47":"0.5749","sample48":"0.371","sample49":"0.4342","sample50":"0","sample51":"0.2184","sample52":"0.2883","sample53":"24.8854","sample54":"25.8482","sample55":"23.5506","sample56":"0.3099","sample57":"0.3855","sample58":"0.3031","sample59":"0","sample60":"0","sample61":"0","sample62":"0","sample63":"0.1029","sample64":"0","sample65":"0","sample66":"0","sample67":"0"},{"Metabolite":"Succinic acid","sample1":"0.0248","sample2":"0.376","sample3":"0.3208","sample4":"0.4332","sample5":"0.163","sample6":"0.2138","sample7":"0.2288","sample8":"0.1093","sample9":"0.0293","sample10":"0.0308","sample11":"0.3504","sample12":"0.2983","sample13":"0.3699","sample14":"0.1198","sample15":"0.1162","sample16":"0.1043","sample17":"0.0659","sample18":"0.0752","sample19":"0.0835","sample20":"0.4123","sample21":"0.3888","sample22":"0.3608","sample23":"0.0696","sample24":"0.0748","sample25":"0.1177","sample26":"0.043","sample27":"0.0619","sample28":"0.04","sample29":"0.159","sample30":"0.2987","sample31":"0.3788","sample32":"0.0212","sample33":"0","sample34":"0.0174","sample35":"0.4413","sample36":"0.4566","sample37":"0.3534","sample38":"0.1152","sample39":"0.1411","sample40":"0.0846","sample41":"0.0716","sample42":"0","sample43":"0","sample44":"0.4591","sample45":"0.4226","sample46":"0.4026","sample47":"0.0672","sample48":"0.0417","sample49":"0.0385","sample50":"0.0375","sample51":"0.0362","sample52":"0.0359","sample53":"0.377","sample54":"0.2932","sample55":"0.2614","sample56":"0.0254","sample57":"0.0307","sample58":"0.0135","sample59":"0.0265","sample60":"0.0253","sample61":"0.0167","sample62":"0.4009","sample63":"0.532","sample64":"0.4034","sample65":"0","sample66":"0.0182","sample67":"0.0179"},{"Metabolite":"Glycine","sample1":"0.0778","sample2":"0.1094","sample3":"0.0094","sample4":"0.0081","sample5":"0.0101","sample6":"0","sample7":"0.0082","sample8":"1.1934","sample9":"0.8007","sample10":"1.0387","sample11":"0.0077","sample12":"0.0068","sample13":"0.0064","sample14":"0.4012","sample15":"0.35","sample16":"0.3327","sample17":"0.203","sample18":"0.2017","sample19":"0.1975","sample20":"0.0014","sample21":"0","sample22":"0","sample23":"0.2538","sample24":"0.2634","sample25":"0.3041","sample26":"0.176","sample27":"0.198","sample28":"0.1708","sample29":"0.6765","sample30":"0.8657","sample31":"0.8305","sample32":"0.0714","sample33":"0.0644","sample34":"0.0578","sample35":"0.0053","sample36":"0.0053","sample37":"0.0072","sample38":"0.1564","sample39":"0.0074","sample40":"0.3186","sample41":"0.7397","sample42":"0.9807","sample43":"0.9317","sample44":"0.0082","sample45":"0.0022","sample46":"0","sample47":"0.3648","sample48":"0.1991","sample49":"0.0131","sample50":"0.1417","sample51":"0.1375","sample52":"0.4763","sample53":"0","sample54":"0","sample55":"0","sample56":"0.1921","sample57":"0.2459","sample58":"0.1925","sample59":"0.1289","sample60":"0.1465","sample61":"0.055","sample62":"0.66","sample63":"0.0072","sample64":"0.0072","sample65":"0.0588","sample66":"0.07","sample67":"0.0657"},{"Metabolite":"Glutamic acid","sample1":"0","sample2":"0","sample3":"0","sample4":"0","sample5":"0","sample6":"0","sample7":"0","sample8":"0.7505","sample9":"0.5168","sample10":"0.5104","sample11":"0","sample12":"0","sample13":"0","sample14":"0","sample15":"0","sample16":"0","sample17":"0","sample18":"0","sample19":"0","sample20":"0","sample21":"0","sample22":"0","sample23":"0","sample24":"0","sample25":"0","sample26":"0","sample27":"0","sample28":"0","sample29":"0","sample30":"0.5116","sample31":"0.2741","sample32":"0","sample33":"0","sample34":"0","sample35":"0","sample36":"0","sample37":"0","sample38":"0","sample39":"0","sample40":"0","sample41":"0.7853","sample42":"0.3403","sample43":"0.6926","sample44":"0","sample45":"0","sample46":"0","sample47":"0","sample48":"0","sample49":"0","sample50":"0","sample51":"0","sample52":"0.5707","sample53":"0","sample54":"0","sample55":"0","sample56":"0","sample57":"0","sample58":"0","sample59":"0","sample60":"0","sample61":"0","sample62":"0","sample63":"0","sample64":"0","sample65":"0","sample66":"0","sample67":"0"},{"Metabolite":"Valine","sample1":"0","sample2":"0.1013","sample3":"0.0751","sample4":"0.0787","sample5":"0.047","sample6":"0.0547","sample7":"0.0611","sample8":"0.0542","sample9":"0.039","sample10":"0.0482","sample11":"0.0805","sample12":"0.0662","sample13":"0.0722","sample14":"0.0362","sample15":"0.0367","sample16":"0.0299","sample17":"0.0264","sample18":"0.0268","sample19":"0.0236","sample20":"0.0898","sample21":"0.0874","sample22":"0.0892","sample23":"0.0262","sample24":"0.03","sample25":"0.0375","sample26":"0.025","sample27":"0.0305","sample28":"0.0287","sample29":"0.025","sample30":"0.0573","sample31":"0.0666","sample32":"0","sample33":"0","sample34":"0","sample35":"0.1001","sample36":"0.0944","sample37":"0.0697","sample38":"0.0184","sample39":"0.0262","sample40":"0.0255","sample41":"0.0454","sample42":"0.0441","sample43":"0.0465","sample44":"0.0883","sample45":"0.0922","sample46":"0.1014","sample47":"0.0197","sample48":"0.0139","sample49":"0.0186","sample50":"0.0276","sample51":"0.0266","sample52":"0.0461","sample53":"0.0775","sample54":"0.0705","sample55":"0.0676","sample56":"0.0144","sample57":"0.0159","sample58":"0.0166","sample59":"0.0176","sample60":"0.0527","sample61":"0.0017","sample62":"0.0688","sample63":"0.0767","sample64":"0.0571","sample65":"0","sample66":"0","sample67":"0"},{"Metabolite":"Glucose","sample1":"0","sample2":"0","sample3":"0","sample4":"0","sample5":"0","sample6":"0","sample7":"0","sample8":"0","sample9":"0","sample10":"0","sample11":"0","sample12":"0","sample13":"0","sample14":"0","sample15":"0","sample16":"0","sample17":"4.8326","sample18":"5.3765","sample19":"4.8441","sample20":"0","sample21":"0","sample22":"0","sample23":"0","sample24":"0","sample25":"0","sample26":"11.6622","sample27":"13.972","sample28":"11.4724","sample29":"0","sample30":"0","sample31":"0","sample32":"0","sample33":"0","sample34":"0","sample35":"0","sample36":"0","sample37":"0","sample38":"0","sample39":"0","sample40":"0","sample41":"0","sample42":"0","sample43":"0","sample44":"0","sample45":"0","sample46":"0","sample47":"0","sample48":"0","sample49":"0","sample50":"6.2758","sample51":"6.0624","sample52":"4.4247","sample53":"0","sample54":"0","sample55":"0","sample56":"0","sample57":"0","sample58":"0","sample59":"13.6535","sample60":"13.4713","sample61":"19.3396","sample62":"0","sample63":"0","sample64":"0","sample65":"0","sample66":"0","sample67":"0"},{"Metabolite":"Tyrosine","sample1":"0.0249","sample2":"0","sample3":"0","sample4":"0","sample5":"0","sample6":"0","sample7":"0","sample8":"0","sample9":"0.0607","sample10":"0.0356","sample11":"0","sample12":"0","sample13":"0","sample14":"0.0633","sample15":"0.0494","sample16":"0.04","sample17":"0.0464","sample18":"0.0515","sample19":"0.0421","sample20":"0","sample21":"0","sample22":"0","sample23":"0.0445","sample24":"0.0379","sample25":"0.0479","sample26":"0.0435","sample27":"0.0467","sample28":"0.0435","sample29":"0","sample30":"0.0281","sample31":"0.0098","sample32":"0","sample33":"0","sample34":"0.0136","sample35":"0","sample36":"0","sample37":"0","sample38":"0","sample39":"0","sample40":"0","sample41":"0.0737","sample42":"0","sample43":"0.052","sample44":"0","sample45":"0","sample46":"0","sample47":"0.0719","sample48":"0.0439","sample49":"0.0504","sample50":"0.049","sample51":"0.0468","sample52":"0.0496","sample53":"0","sample54":"0","sample55":"0","sample56":"0.0388","sample57":"0.0516","sample58":"0.0413","sample59":"0.0491","sample60":"0.0446","sample61":"0.0134","sample62":"0","sample63":"0","sample64":"0","sample65":"0","sample66":"0","sample67":"0.0156"},{"Metabolite":"Arginine","sample1":"0","sample2":"0","sample3":"0","sample4":"0","sample5":"0","sample6":"0","sample7":"0","sample8":"0","sample9":"0","sample10":"0","sample11":"0","sample12":"0","sample13":"0","sample14":"0","sample15":"0","sample16":"0","sample17":"0","sample18":"0","sample19":"0","sample20":"0","sample21":"0","sample22":"0","sample23":"2.3074","sample24":"0.0996","sample25":"3.2188","sample26":"0","sample27":"0","sample28":"0","sample29":"0","sample30":"0","sample31":"0","sample32":"0","sample33":"0","sample34":"0","sample35":"0","sample36":"0","sample37":"0","sample38":"0","sample39":"0","sample40":"0","sample41":"0","sample42":"0","sample43":"0","sample44":"0","sample45":"0","sample46":"0","sample47":"0.2259","sample48":"0","sample49":"0.0664","sample50":"0","sample51":"0","sample52":"0","sample53":"0","sample54":"0","sample55":"0","sample56":"0.0963","sample57":"0","sample58":"0.7123","sample59":"0","sample60":"0","sample61":"0","sample62":"0","sample63":"0","sample64":"0","sample65":"0","sample66":"0","sample67":"0"}],

"Metabolites":[{"Metabolite":"5-Aminopentanoic acid","Sterile Saliva":"0","P10 1 D4":"1.5654","P10 2 D4":"1.2133","P10 3 D4":"1.497","A10 1 D4":"0.9911","A10 2 D4":"0.6478","A10 3 D4":"0.6567","G10 1 D4":"0.8604","G10 2 D4":"0.607","G10 3 D4":"0.7329","P25 1 D4":"1.4586","P25 2 D4":"1.082","P25 3 D4":"1.3326","A25 1 D4":"0.1244","A25 2 D4":"0.1105","A25 3 D4":"0.0909","G25 1 D4":"0","G25 2 D4":"0.1831","G25 3 D4":"0.171","P50 1 D4":"1.3569","P50 2 D4":"1.4923","P50 3 D4":"1.2555","A50 1 D4":"0.0926","A50 2 D4":"0.1034","A50 3 D4":"0.1","G50 1 D4":"0","G50 2 D4":"0.141","G50 3 D4":"0","Unsupplemented Control 1 D4":"1.2992","Unsupplemented Control 2 D4":"1.3429","Unsupplemented Control 3 D4":"1.0774","Negative Control 1 D4":"0.1125","Negative Control 2 D4":"0.097","Negative Control 3 D4":"0.0942","P10 1 D7":"0.7139","P10 2 D7":"0.479","P10 3 D7":"0.8383","A10 1 D7":"1.244","A10 2 D7":"1.0811","A10 3 D7":"1.2903","G10 1 D7":"0.3094","G10 2 D7":"0.4528","G10 3 D7":"0.4444","P25 1 D7":"0.8435","P25 2 D7":"0.993","P25 3 D7":"0.7779","A25 1 D7":"0.136","A25 2 D7":"0.0926","A25 3 D7":"0.0944","G25 1 D7":"0.0966","G25 2 D7":"0.0894","G25 3 D7":"0.3468","P50 1 D7":"0.9219","P50 2 D7":"1.0341","P50 3 D7":"0.9383","A50 1 D7":"0.0831","A50 2 D7":"0.1027","A50 3 D7":"0.0821","G50 1 D7":"0.081","G50 2 D7":"0","G50 3 D7":"0.104","Unsupplemented Control 1 D7":"2.3716","Unsupplemented Control 2 D7":"2.4505","Unsupplemented Control 3 D7":"1.7254","Negative Control 1 D7":"0.0909","Negative Control 2 D7":"0","Negative Control 3 D7":"0.1122"},{"Metabolite":"Propionic acid","Sterile Saliva":"0.2998","P10 1 D4":"0.4734","P10 2 D4":"0.4305","P10 3 D4":"0.4649","A10 1 D4":"0.3957","A10 2 D4":"0.4064","A10 3 D4":"0.4493","G10 1 D4":"0.2783","G10 2 D4":"0.5404","G10 3 D4":"0.7805","P25 1 D4":"0.514","P25 2 D4":"0.3812","P25 3 D4":"0.4689","A25 1 D4":"0.3322","A25 2 D4":"0.3114","A25 3 D4":"0.2631","G25 1 D4":"0.1716","G25 2 D4":"0.1792","G25 3 D4":"0.1736","P50 1 D4":"0.5653","P50 2 D4":"0.5713","P50 3 D4":"0.6098","A50 1 D4":"0.2473","A50 2 D4":"0.2697","A50 3 D4":"0.2945","G50 1 D4":"0.1411","G50 2 D4":"0.1638","G50 3 D4":"0.1614","Unsupplemented Control 1 D4":"0.3945","Unsupplemented Control 2 D4":"0.3777","Unsupplemented Control 3 D4":"0.365","Negative Control 1 D4":"0.2722","Negative Control 2 D4":"0.249","Negative Control 3 D4":"0.218","P10 1 D7":"0.7021","P10 2 D7":"0.5379","P10 3 D7":"0.4774","A10 1 D7":"0.2986","A10 2 D7":"0.3133","A10 3 D7":"0.3461","G10 1 D7":"0.2146","G10 2 D7":"1.4394","G10 3 D7":"1.2878","P25 1 D7":"0.6322","P25 2 D7":"0.7363","P25 3 D7":"0.9528","A25 1 D7":"0.3631","A25 2 D7":"0.2198","A25 3 D7":"0.2398","G25 1 D7":"0.1895","G25 2 D7":"0.1785","G25 3 D7":"0.15","P50 1 D7":"0.6691","P50 2 D7":"0.5361","P50 3 D7":"0.6499","A50 1 D7":"0.2283","A50 2 D7":"0.2674","A50 3 D7":"0.2105","G50 1 D7":"0.1843","G50 2 D7":"0.1834","G50 3 D7":"0.2223","Unsupplemented Control 1 D7":"0.3926","Unsupplemented Control 2 D7":"0.4303","Unsupplemented Control 3 D7":"0.2997","Negative Control 1 D7":"0.2271","Negative Control 2 D7":"0.13","Negative Control 3 D7":"0.2255"},{"Metabolite":"Butyric acid","Sterile Saliva":"0","P10 1 D4":"0.8958","P10 2 D4":"0.7544","P10 3 D4":"0.8345","A10 1 D4":"0.5692","A10 2 D4":"0.5254","A10 3 D4":"0.6559","G10 1 D4":"0.0595","G10 2 D4":"0.1175","G10 3 D4":"0.222","P25 1 D4":"0.827","P25 2 D4":"0.7416","P25 3 D4":"0.7288","A25 1 D4":"0.0752","A25 2 D4":"0.0688","A25 3 D4":"0.0505","G25 1 D4":"0.0389","G25 2 D4":"0.0428","G25 3 D4":"0.0361","P50 1 D4":"0.9427","P50 2 D4":"0.9134","P50 3 D4":"0.8441","A50 1 D4":"0.0509","A50 2 D4":"0.0558","A50 3 D4":"0.0613","G50 1 D4":"0.0256","G50 2 D4":"0.0283","G50 3 D4":"0.0284","Unsupplemented Control 1 D4":"0.496","Unsupplemented Control 2 D4":"0.1439","Unsupplemented Control 3 D4":"0.324","Negative Control 1 D4":"0.0389","Negative Control 2 D4":"0.037","Negative Control 3 D4":"0.0338","P10 1 D7":"1.2285","P10 2 D7":"1.1275","P10 3 D7":"0.8096","A10 1 D7":"0.4204","A10 2 D7":"0.3626","A10 3 D7":"0.366","G10 1 D7":"0.0482","G10 2 D7":"0.3053","G10 3 D7":"0.0518","P25 1 D7":"0.9916","P25 2 D7":"1.0682","P25 3 D7":"1.1235","A25 1 D7":"0.0777","A25 2 D7":"0.0442","A25 3 D7":"0.0495","G25 1 D7":"0.0359","G25 2 D7":"0.032","G25 3 D7":"0.0358","P50 1 D7":"0.9988","P50 2 D7":"0.9367","P50 3 D7":"0.8815","A50 1 D7":"0.0452","A50 2 D7":"0.0563","A50 3 D7":"0.0437","G50 1 D7":"0.0389","G50 2 D7":"0.0311","G50 3 D7":"0.0375","Unsupplemented Control 1 D7":"0.9169","Unsupplemented Control 2 D7":"0.8141","Unsupplemented Control 3 D7":"0.6562","Negative Control 1 D7":"0.0333","Negative Control 2 D7":"0","Negative Control 3 D7":"0.0345"},{"Metabolite":"Formic acid","Sterile Saliva":"0.0985","P10 1 D4":"2.6695","P10 2 D4":"1.6217","P10 3 D4":"0.5505","A10 1 D4":"1.5973","A10 2 D4":"1.4291","A10 3 D4":"1.3268","G10 1 D4":"4.5712","G10 2 D4":"3.4475","G10 3 D4":"4.7489","P25 1 D4":"1.7439","P25 2 D4":"1.0552","P25 3 D4":"1.0519","A25 1 D4":"1.19","A25 2 D4":"1.2907","A25 3 D4":"0.9776","G25 1 D4":"0.4044","G25 2 D4":"0.7437","G25 3 D4":"0.8219","P50 1 D4":"1.3699","P50 2 D4":"1.1744","P50 3 D4":"0.68","A50 1 D4":"0.8192","A50 2 D4":"0.7415","A50 3 D4":"1.1531","G50 1 D4":"0.2516","G50 2 D4":"0.4002","G50 3 D4":"0.2569","Unsupplemented Control 1 D4":"0.1132","Unsupplemented Control 2 D4":"0.7764","Unsupplemented Control 3 D4":"0.9212","Negative Control 1 D4":"0.0845","Negative Control 2 D4":"0.0628","Negative Control 3 D4":"0.0783","P10 1 D7":"0.244","P10 2 D7":"0.0019","P10 3 D7":"0","A10 1 D7":"0.2049","A10 2 D7":"0.512","A10 3 D7":"1.2597","G10 1 D7":"1.7747","G10 2 D7":"4.0336","G10 3 D7":"3.1813","P25 1 D7":"0.1744","P25 2 D7":"0.0801","P25 3 D7":"0.1304","A25 1 D7":"0.8626","A25 2 D7":"0.5828","A25 3 D7":"1.0689","G25 1 D7":"0.1239","G25 2 D7":"0.055","G25 3 D7":"0.2983","P50 1 D7":"0.0068","P50 2 D7":"0.0451","P50 3 D7":"0.0335","A50 1 D7":"0.611","A50 2 D7":"0.6522","A50 3 D7":"0.5172","G50 1 D7":"0.0385","G50 2 D7":"0.5788","G50 3 D7":"0.0732","Unsupplemented Control 1 D7":"0.7838","Unsupplemented Control 2 D7":"0.5065","Unsupplemented Control 3 D7":"0.6417","Negative Control 1 D7":"0","Negative Control 2 D7":"0.0888","Negative Control 3 D7":"0.0784"},{"Metabolite":"Ethanol","Sterile Saliva":"0","P10 1 D4":"0.2079","P10 2 D4":"0.1804","P10 3 D4":"0.1983","A10 1 D4":"0.2056","A10 2 D4":"0.2583","A10 3 D4":"0.2702","G10 1 D4":"0.4515","G10 2 D4":"0.3359","G10 3 D4":"0.4398","P25 1 D4":"0.133","P25 2 D4":"0.1103","P25 3 D4":"0.125","A25 1 D4":"0.5247","A25 2 D4":"0.4738","A25 3 D4":"0.402","G25 1 D4":"0.4251","G25 2 D4":"0.4126","G25 3 D4":"0.4171","P50 1 D4":"0.1532","P50 2 D4":"0.1484","P50 3 D4":"0.1535","A50 1 D4":"0.3958","A50 2 D4":"0.4094","A50 3 D4":"0.4352","G50 1 D4":"0.2651","G50 2 D4":"0.3242","G50 3 D4":"0.281","Unsupplemented Control 1 D4":"0.2261","Unsupplemented Control 2 D4":"0.2119","Unsupplemented Control 3 D4":"0.235","Negative Control 1 D4":"0.4181","Negative Control 2 D4":"0.3854","Negative Control 3 D4":"0.3166","P10 1 D7":"0.7769","P10 2 D7":"0.7835","P10 3 D7":"0.6233","A10 1 D7":"0.7268","A10 2 D7":"0.7109","A10 3 D7":"0.7454","G10 1 D7":"0.8141","G10 2 D7":"0.794","G10 3 D7":"0.8607","P25 1 D7":"0.5354","P25 2 D7":"0.663","P25 3 D7":"0.6988","A25 1 D7":"1.2266","A25 2 D7":"0.7867","A25 3 D7":"0.8576","G25 1 D7":"0.9935","G25 2 D7":"0.9409","G25 3 D7":"1.0338","P50 1 D7":"0.4825","P50 2 D7":"0.4774","P50 3 D7":"0.5208","A50 1 D7":"0.8509","A50 2 D7":"0.862","A50 3 D7":"0.757","G50 1 D7":"0.8758","G50 2 D7":"0.8921","G50 3 D7":"1.0411","Unsupplemented Control 1 D7":"1.0385","Unsupplemented Control 2 D7":"1.0762","Unsupplemented Control 3 D7":"1.0609","Negative Control 1 D7":"1.2718","Negative Control 2 D7":"0.897","Negative Control 3 D7":"1.1264"},{"Metabolite":"Acetic acid","Sterile Saliva":"2.1818","P10 1 D4":"9.021","P10 2 D4":"7.9856","P10 3 D4":"8.7387","A10 1 D4":"6.9626","A10 2 D4":"7.2142","A10 3 D4":"8.1821","G10 1 D4":"7.4442","G10 2 D4":"6.1854","G10 3 D4":"7.9605","P25 1 D4":"8.6239","P25 2 D4":"7.3983","P25 3 D4":"7.7907","A25 1 D4":"4.492","A25 2 D4":"4.0641","A25 3 D4":"3.4935","G25 1 D4":"2.2735","G25 2 D4":"2.5761","G25 3 D4":"2.6142","P50 1 D4":"9.5797","P50 2 D4":"9.3113","P50 3 D4":"8.8818","A50 1 D4":"3.2345","A50 2 D4":"3.56","A50 3 D4":"3.9599","G50 1 D4":"1.6903","G50 2 D4":"2.2496","G50 3 D4":"1.7313","Unsupplemented Control 1 D4":"6.377","Unsupplemented Control 2 D4":"6.0199","Unsupplemented Control 3 D4":"6.9844","Negative Control 1 D4":"2.0251","Negative Control 2 D4":"1.8528","Negative Control 3 D4":"1.6413","P10 1 D7":"12.1875","P10 2 D7":"10.6843","P10 3 D7":"9.2826","A10 1 D7":"5.9131","A10 2 D7":"6.332","A10 3 D7":"6.9559","G10 1 D7":"4.1082","G10 2 D7":"7.6525","G10 3 D7":"6.5859","P25 1 D7":"11.194","P25 2 D7":"11.8059","P25 3 D7":"11.9926","A25 1 D7":"4.1478","A25 2 D7":"2.6911","A25 3 D7":"3.3318","G25 1 D7":"1.5863","G25 2 D7":"1.4287","G25 3 D7":"1.7548","P50 1 D7":"9.9783","P50 2 D7":"10.437","P50 3 D7":"9.5848","A50 1 D7":"2.6612","A50 2 D7":"3.2183","A50 3 D7":"2.5034","G50 1 D7":"1.4363","G50 2 D7":"1.8431","G50 3 D7":"1.6418","Unsupplemented Control 1 D7":"10.0832","Unsupplemented Control 2 D7":"10.0404","Unsupplemented Control 3 D7":"8.2539","Negative Control 1 D7":"1.7943","Negative Control 2 D7":"1.8702","Negative Control 3 D7":"1.7765"},{"Metabolite":"Phenylalanine","Sterile Saliva":"0","P10 1 D4":"0.0589","P10 2 D4":"0.0534","P10 3 D4":"0.0573","A10 1 D4":"0.0391","A10 2 D4":"0.0451","A10 3 D4":"0.0527","G10 1 D4":"0.0526","G10 2 D4":"0.0379","G10 3 D4":"0.0446","P25 1 D4":"0.062","P25 2 D4":"0.0511","P25 3 D4":"0.0522","A25 1 D4":"0.0485","A25 2 D4":"0.0419","A25 3 D4":"0.0336","G25 1 D4":"0.0278","G25 2 D4":"0.0269","G25 3 D4":"0.0249","P50 1 D4":"0.0542","P50 2 D4":"0.0594","P50 3 D4":"0.0559","A50 1 D4":"0.0363","A50 2 D4":"0.035","A50 3 D4":"0.0391","G50 1 D4":"0.0251","G50 2 D4":"0.0286","G50 3 D4":"0.0261","Unsupplemented Control 1 D4":"0","Unsupplemented Control 2 D4":"0","Unsupplemented Control 3 D4":"0.0529","Negative Control 1 D4":"0","Negative Control 2 D4":"0","Negative Control 3 D4":"0","P10 1 D7":"0.0604","P10 2 D7":"0.0642","P10 3 D7":"0.0461","A10 1 D7":"0.0324","A10 2 D7":"0.0334","A10 3 D7":"0.0365","G10 1 D7":"0.0343","G10 2 D7":"0.0436","G10 3 D7":"0.0336","P25 1 D7":"0.0531","P25 2 D7":"0.0641","P25 3 D7":"0.0729","A25 1 D7":"0.0438","A25 2 D7":"0","A25 3 D7":"0.0304","G25 1 D7":"0.0331","G25 2 D7":"0.0325","G25 3 D7":"0.0331","P50 1 D7":"0.0546","P50 2 D7":"0.0521","P50 3 D7":"0.0473","A50 1 D7":"0.0258","A50 2 D7":"0.0329","A50 3 D7":"0.0268","G50 1 D7":"0.0311","G50 2 D7":"0.0304","G50 3 D7":"0.0065","Unsupplemented Control 1 D7":"0.0476","Unsupplemented Control 2 D7":"0.0556","Unsupplemented Control 3 D7":"0.0318","Negative Control 1 D7":"0","Negative Control 2 D7":"0","Negative Control 3 D7":"0"},{"Metabolite":"Alanine","Sterile Saliva":"0.019","P10 1 D4":"0","P10 2 D4":"0","P10 3 D4":"0","A10 1 D4":"0","A10 2 D4":"0.0759","A10 3 D4":"0.1358","G10 1 D4":"0.1603","G10 2 D4":"0.1134","G10 3 D4":"0.1297","P25 1 D4":"0","P25 2 D4":"0","P25 3 D4":"0","A25 1 D4":"0.2194","A25 2 D4":"0.1774","A25 3 D4":"0.1454","G25 1 D4":"0.0804","G25 2 D4":"0.0727","G25 3 D4":"0.0656","P50 1 D4":"0","P50 2 D4":"0","P50 3 D4":"0","A50 1 D4":"0.135","A50 2 D4":"0.1469","A50 3 D4":"0.156","G50 1 D4":"0","G50 2 D4":"0.0817","G50 3 D4":"0","Unsupplemented Control 1 D4":"0","Unsupplemented Control 2 D4":"0","Unsupplemented Control 3 D4":"0","Negative Control 1 D4":"0.016","Negative Control 2 D4":"0.0146","Negative Control 3 D4":"0.0133","P10 1 D7":"0","P10 2 D7":"0","P10 3 D7":"0","A10 1 D7":"0.0233","A10 2 D7":"0","A10 3 D7":"0.0316","G10 1 D7":"0.1045","G10 2 D7":"0.1204","G10 3 D7":"0.0694","P25 1 D7":"0","P25 2 D7":"0","P25 3 D7":"0","A25 1 D7":"0.177","A25 2 D7":"0.1031","A25 3 D7":"0.1423","G25 1 D7":"0.0542","G25 2 D7":"0","G25 3 D7":"0.0767","P50 1 D7":"0","P50 2 D7":"0","P50 3 D7":"0","A50 1 D7":"0.0946","A50 2 D7":"0.1688","A50 3 D7":"0.114","G50 1 D7":"0","G50 2 D7":"0","G50 3 D7":"0.0098","Unsupplemented Control 1 D7":"0","Unsupplemented Control 2 D7":"0","Unsupplemented Control 3 D7":"0","Negative Control 1 D7":"0.0129","Negative Control 2 D7":"0","Negative Control 3 D7":"0.0146"},{"Metabolite":"Lactic acid","Sterile Saliva":"0.1683","P10 1 D4":"0.0048","P10 2 D4":"0.0039","P10 3 D4":"0.0086","A10 1 D4":"0.0073","A10 2 D4":"0.0267","A10 3 D4":"0.0281","G10 1 D4":"8.6563","G10 2 D4":"5.8085","G10 3 D4":"7.1043","P25 1 D4":"0.0064","P25 2 D4":"0.0037","P25 3 D4":"0.004","A25 1 D4":"0.2784","A25 2 D4":"0.1667","A25 3 D4":"0.1613","G25 1 D4":"13.3291","G25 2 D4":"13.5382","G25 3 D4":"13.1396","P50 1 D4":"0.006","P50 2 D4":"0.0039","P50 3 D4":"0.004","A50 1 D4":"0.2619","A50 2 D4":"0.2844","A50 3 D4":"0.1352","G50 1 D4":"11.0059","G50 2 D4":"13.6119","G50 3 D4":"13.4516","Unsupplemented Control 1 D4":"0.006","Unsupplemented Control 2 D4":"0.0188","Unsupplemented Control 3 D4":"0.0064","Negative Control 1 D4":"0.1465","Negative Control 2 D4":"0.1356","Negative Control 3 D4":"0.1201","P10 1 D7":"0.0047","P10 2 D7":"0.0037","P10 3 D7":"0.004","A10 1 D7":"0.0155","A10 2 D7":"0.0082","A10 3 D7":"0.0164","G10 1 D7":"10.2342","G10 2 D7":"7.9777","G10 3 D7":"7.8013","P25 1 D7":"0.0074","P25 2 D7":"0.0044","P25 3 D7":"0.0046","A25 1 D7":"0.4477","A25 2 D7":"0.2255","A25 3 D7":"0.3623","G25 1 D7":"15.1106","G25 2 D7":"13.1143","G25 3 D7":"14.6809","P50 1 D7":"0.0126","P50 2 D7":"0","P50 3 D7":"0","A50 1 D7":"0.2508","A50 2 D7":"0.2595","A50 3 D7":"0.1795","G50 1 D7":"10.4028","G50 2 D7":"13.0744","G50 3 D7":"0.1516","Unsupplemented Control 1 D7":"0.0082","Unsupplemented Control 2 D7":"0.0057","Unsupplemented Control 3 D7":"0.0103","Negative Control 1 D7":"0.124","Negative Control 2 D7":"0.1184","Negative Control 3 D7":"0.1246"},{"Metabolite":"Putrescine","Sterile Saliva":"0","P10 1 D4":"0.2748","P10 2 D4":"0.2358","P10 3 D4":"0.2553","A10 1 D4":"3.3936","A10 2 D4":"3.8634","A10 3 D4":"3.7586","G10 1 D4":"0","G10 2 D4":"0","G10 3 D4":"0","P25 1 D4":"0.2622","P25 2 D4":"0.2306","P25 3 D4":"0.2518","A25 1 D4":"5.7624","A25 2 D4":"4.7715","A25 3 D4":"4.4849","G25 1 D4":"0","G25 2 D4":"0","G25 3 D4":"0","P50 1 D4":"0.3011","P50 2 D4":"0.29","P50 3 D4":"0.2777","A50 1 D4":"3.5543","A50 2 D4":"3.7516","A50 3 D4":"4.6764","G50 1 D4":"0","G50 2 D4":"0","G50 3 D4":"0","Unsupplemented Control 1 D4":"0.215","Unsupplemented Control 2 D4":"0.2405","Unsupplemented Control 3 D4":"0.2464","Negative Control 1 D4":"0","Negative Control 2 D4":"0","Negative Control 3 D4":"0","P10 1 D7":"0.3457","P10 2 D7":"0.332","P10 3 D7":"0.2734","A10 1 D7":"2.6905","A10 2 D7":"2.8036","A10 3 D7":"2.4468","G10 1 D7":"0","G10 2 D7":"0","G10 3 D7":"0","P25 1 D7":"0.3223","P25 2 D7":"0.3325","P25 3 D7":"0.4096","A25 1 D7":"5.3743","A25 2 D7":"3.8358","A25 3 D7":"3.3974","G25 1 D7":"0","G25 2 D7":"0","G25 3 D7":"0","P50 1 D7":"0.2659","P50 2 D7":"0.28","P50 3 D7":"0.2412","A50 1 D7":"2.9041","A50 2 D7":"5.5422","A50 3 D7":"4.1946","G50 1 D7":"0","G50 2 D7":"0","G50 3 D7":"0","Unsupplemented Control 1 D7":"0.2824","Unsupplemented Control 2 D7":"0.29","Unsupplemented Control 3 D7":"0.2145","Negative Control 1 D7":"0","Negative Control 2 D7":"0","Negative Control 3 D7":"0"},{"Metabolite":"Methanol","Sterile Saliva":"0.0242","P10 1 D4":"0.1019","P10 2 D4":"0.0864","P10 3 D4":"0.0926","A10 1 D4":"0.0698","A10 2 D4":"0.0872","A10 3 D4":"0.0872","G10 1 D4":"0.0689","G10 2 D4":"0.0516","G10 3 D4":"0.0684","P25 1 D4":"0.6982","P25 2 D4":"0.6049","P25 3 D4":"0.654","A25 1 D4":"0.0951","A25 2 D4":"0.0886","A25 3 D4":"0.0748","G25 1 D4":"0.0727","G25 2 D4":"0.0687","G25 3 D4":"0.0707","P50 1 D4":"1.3552","P50 2 D4":"1.2888","P50 3 D4":"1.2279","A50 1 D4":"0.0731","A50 2 D4":"0.0725","A50 3 D4":"0.0783","G50 1 D4":"0.0693","G50 2 D4":"0.0794","G50 3 D4":"0.0729","Unsupplemented Control 1 D4":"0.0835","Unsupplemented Control 2 D4":"0.0838","Unsupplemented Control 3 D4":"0.087","Negative Control 1 D4":"0.0883","Negative Control 2 D4":"0.0892","Negative Control 3 D4":"0.079","P10 1 D7":"0.1657","P10 2 D7":"0.1685","P10 3 D7":"0.1395","A10 1 D7":"0.1322","A10 2 D7":"0.1278","A10 3 D7":"0.1368","G10 1 D7":"0.1062","G10 2 D7":"0.1051","G10 3 D7":"0.1113","P25 1 D7":"0.8419","P25 2 D7":"0.1888","P25 3 D7":"1.0258","A25 1 D7":"0.1652","A25 2 D7":"0.1044","A25 3 D7":"0.111","G25 1 D7":"0.1209","G25 2 D7":"0.1144","G25 3 D7":"0.1253","P50 1 D7":"1.2542","P50 2 D7":"0.1902","P50 3 D7":"0.227","A50 1 D7":"0.1127","A50 2 D7":"0.1176","A50 3 D7":"0.1011","G50 1 D7":"0.1169","G50 2 D7":"0.1179","G50 3 D7":"0.1339","Unsupplemented Control 1 D7":"0.15","Unsupplemented Control 2 D7":"0.1519","Unsupplemented Control 3 D7":"0.1472","Negative Control 1 D7":"0.1636","Negative Control 2 D7":"0.1327","Negative Control 3 D7":"0.1426"},{"Metabolite":"Proline","Sterile Saliva":"0","P10 1 D4":"6.6555","P10 2 D4":"5.58","P10 3 D4":"6.0444","A10 1 D4":"1.1481","A10 2 D4":"1.3558","A10 3 D4":"1.3206","G10 1 D4":"0","G10 2 D4":"0","G10 3 D4":"0","P25 1 D4":"14.087","P25 2 D4":"12.0003","P25 3 D4":"13.1289","A25 1 D4":"0.6823","A25 2 D4":"0.6464","A25 3 D4":"0.575","G25 1 D4":"0.3187","G25 2 D4":"0.3176","G25 3 D4":"0","P50 1 D4":"25.2884","P50 2 D4":"25.4488","P50 3 D4":"24.0924","A50 1 D4":"0.4117","A50 2 D4":"0.4181","A50 3 D4":"0.5329","G50 1 D4":"0","G50 2 D4":"0","G50 3 D4":"0","Unsupplemented Control 1 D4":"0.1843","Unsupplemented Control 2 D4":"0.4324","Unsupplemented Control 3 D4":"0.8055","Negative Control 1 D4":"0","Negative Control 2 D4":"0","Negative Control 3 D4":"0","P10 1 D7":"9.1519","P10 2 D7":"8.663","P10 3 D7":"5.3857","A10 1 D7":"0","A10 2 D7":"0.4241","A10 3 D7":"0","G10 1 D7":"0.5385","G10 2 D7":"0.3228","G10 3 D7":"0.5624","P25 1 D7":"17.1239","P25 2 D7":"18.2361","P25 3 D7":"19.778","A25 1 D7":"0.5749","A25 2 D7":"0.371","A25 3 D7":"0.4342","G25 1 D7":"0","G25 2 D7":"0.2184","G25 3 D7":"0.2883","P50 1 D7":"24.8854","P50 2 D7":"25.8482","P50 3 D7":"23.5506","A50 1 D7":"0.3099","A50 2 D7":"0.3855","A50 3 D7":"0.3031","G50 1 D7":"0","G50 2 D7":"0","G50 3 D7":"0","Unsupplemented Control 1 D7":"0","Unsupplemented Control 2 D7":"0.1029","Unsupplemented Control 3 D7":"0","Negative Control 1 D7":"0","Negative Control 2 D7":"0","Negative Control 3 D7":"0"},{"Metabolite":"Succinic acid","Sterile Saliva":"0.0248","P10 1 D4":"0.376","P10 2 D4":"0.3208","P10 3 D4":"0.4332","A10 1 D4":"0.163","A10 2 D4":"0.2138","A10 3 D4":"0.2288","G10 1 D4":"0.1093","G10 2 D4":"0.0293","G10 3 D4":"0.0308","P25 1 D4":"0.3504","P25 2 D4":"0.2983","P25 3 D4":"0.3699","A25 1 D4":"0.1198","A25 2 D4":"0.1162","A25 3 D4":"0.1043","G25 1 D4":"0.0659","G25 2 D4":"0.0752","G25 3 D4":"0.0835","P50 1 D4":"0.4123","P50 2 D4":"0.3888","P50 3 D4":"0.3608","A50 1 D4":"0.0696","A50 2 D4":"0.0748","A50 3 D4":"0.1177","G50 1 D4":"0.043","G50 2 D4":"0.0619","G50 3 D4":"0.04","Unsupplemented Control 1 D4":"0.159","Unsupplemented Control 2 D4":"0.2987","Unsupplemented Control 3 D4":"0.3788","Negative Control 1 D4":"0.0212","Negative Control 2 D4":"0","Negative Control 3 D4":"0.0174","P10 1 D7":"0.4413","P10 2 D7":"0.4566","P10 3 D7":"0.3534","A10 1 D7":"0.1152","A10 2 D7":"0.1411","A10 3 D7":"0.0846","G10 1 D7":"0.0716","G10 2 D7":"0","G10 3 D7":"0","P25 1 D7":"0.4591","P25 2 D7":"0.4226","P25 3 D7":"0.4026","A25 1 D7":"0.0672","A25 2 D7":"0.0417","A25 3 D7":"0.0385","G25 1 D7":"0.0375","G25 2 D7":"0.0362","G25 3 D7":"0.0359","P50 1 D7":"0.377","P50 2 D7":"0.2932","P50 3 D7":"0.2614","A50 1 D7":"0.0254","A50 2 D7":"0.0307","A50 3 D7":"0.0135","G50 1 D7":"0.0265","G50 2 D7":"0.0253","G50 3 D7":"0.0167","Unsupplemented Control 1 D7":"0.4009","Unsupplemented Control 2 D7":"0.532","Unsupplemented Control 3 D7":"0.4034","Negative Control 1 D7":"0","Negative Control 2 D7":"0.0182","Negative Control 3 D7":"0.0179"},{"Metabolite":"Glycine","Sterile Saliva":"0.0778","P10 1 D4":"0.1094","P10 2 D4":"0.0094","P10 3 D4":"0.0081","A10 1 D4":"0.0101","A10 2 D4":"0","A10 3 D4":"0.0082","G10 1 D4":"1.1934","G10 2 D4":"0.8007","G10 3 D4":"1.0387","P25 1 D4":"0.0077","P25 2 D4":"0.0068","P25 3 D4":"0.0064","A25 1 D4":"0.4012","A25 2 D4":"0.35","A25 3 D4":"0.3327","G25 1 D4":"0.203","G25 2 D4":"0.2017","G25 3 D4":"0.1975","P50 1 D4":"0.0014","P50 2 D4":"0","P50 3 D4":"0","A50 1 D4":"0.2538","A50 2 D4":"0.2634","A50 3 D4":"0.3041","G50 1 D4":"0.176","G50 2 D4":"0.198","G50 3 D4":"0.1708","Unsupplemented Control 1 D4":"0.6765","Unsupplemented Control 2 D4":"0.8657","Unsupplemented Control 3 D4":"0.8305","Negative Control 1 D4":"0.0714","Negative Control 2 D4":"0.0644","Negative Control 3 D4":"0.0578","P10 1 D7":"0.0053","P10 2 D7":"0.0053","P10 3 D7":"0.0072","A10 1 D7":"0.1564","A10 2 D7":"0.0074","A10 3 D7":"0.3186","G10 1 D7":"0.7397","G10 2 D7":"0.9807","G10 3 D7":"0.9317","P25 1 D7":"0.0082","P25 2 D7":"0.0022","P25 3 D7":"0","A25 1 D7":"0.3648","A25 2 D7":"0.1991","A25 3 D7":"0.0131","G25 1 D7":"0.1417","G25 2 D7":"0.1375","G25 3 D7":"0.4763","P50 1 D7":"0","P50 2 D7":"0","P50 3 D7":"0","A50 1 D7":"0.1921","A50 2 D7":"0.2459","A50 3 D7":"0.1925","G50 1 D7":"0.1289","G50 2 D7":"0.1465","G50 3 D7":"0.055","Unsupplemented Control 1 D7":"0.66","Unsupplemented Control 2 D7":"0.0072","Unsupplemented Control 3 D7":"0.0072","Negative Control 1 D7":"0.0588","Negative Control 2 D7":"0.07","Negative Control 3 D7":"0.0657"},{"Metabolite":"Glutamic acid","Sterile Saliva":"0","P10 1 D4":"0","P10 2 D4":"0","P10 3 D4":"0","A10 1 D4":"0","A10 2 D4":"0","A10 3 D4":"0","G10 1 D4":"0.7505","G10 2 D4":"0.5168","G10 3 D4":"0.5104","P25 1 D4":"0","P25 2 D4":"0","P25 3 D4":"0","A25 1 D4":"0","A25 2 D4":"0","A25 3 D4":"0","G25 1 D4":"0","G25 2 D4":"0","G25 3 D4":"0","P50 1 D4":"0","P50 2 D4":"0","P50 3 D4":"0","A50 1 D4":"0","A50 2 D4":"0","A50 3 D4":"0","G50 1 D4":"0","G50 2 D4":"0","G50 3 D4":"0","Unsupplemented Control 1 D4":"0","Unsupplemented Control 2 D4":"0.5116","Unsupplemented Control 3 D4":"0.2741","Negative Control 1 D4":"0","Negative Control 2 D4":"0","Negative Control 3 D4":"0","P10 1 D7":"0","P10 2 D7":"0","P10 3 D7":"0","A10 1 D7":"0","A10 2 D7":"0","A10 3 D7":"0","G10 1 D7":"0.7853","G10 2 D7":"0.3403","G10 3 D7":"0.6926","P25 1 D7":"0","P25 2 D7":"0","P25 3 D7":"0","A25 1 D7":"0","A25 2 D7":"0","A25 3 D7":"0","G25 1 D7":"0","G25 2 D7":"0","G25 3 D7":"0.5707","P50 1 D7":"0","P50 2 D7":"0","P50 3 D7":"0","A50 1 D7":"0","A50 2 D7":"0","A50 3 D7":"0","G50 1 D7":"0","G50 2 D7":"0","G50 3 D7":"0","Unsupplemented Control 1 D7":"0","Unsupplemented Control 2 D7":"0","Unsupplemented Control 3 D7":"0","Negative Control 1 D7":"0","Negative Control 2 D7":"0","Negative Control 3 D7":"0"},{"Metabolite":"Valine","Sterile Saliva":"0","P10 1 D4":"0.1013","P10 2 D4":"0.0751","P10 3 D4":"0.0787","A10 1 D4":"0.047","A10 2 D4":"0.0547","A10 3 D4":"0.0611","G10 1 D4":"0.0542","G10 2 D4":"0.039","G10 3 D4":"0.0482","P25 1 D4":"0.0805","P25 2 D4":"0.0662","P25 3 D4":"0.0722","A25 1 D4":"0.0362","A25 2 D4":"0.0367","A25 3 D4":"0.0299","G25 1 D4":"0.0264","G25 2 D4":"0.0268","G25 3 D4":"0.0236","P50 1 D4":"0.0898","P50 2 D4":"0.0874","P50 3 D4":"0.0892","A50 1 D4":"0.0262","A50 2 D4":"0.03","A50 3 D4":"0.0375","G50 1 D4":"0.025","G50 2 D4":"0.0305","G50 3 D4":"0.0287","Unsupplemented Control 1 D4":"0.025","Unsupplemented Control 2 D4":"0.0573","Unsupplemented Control 3 D4":"0.0666","Negative Control 1 D4":"0","Negative Control 2 D4":"0","Negative Control 3 D4":"0","P10 1 D7":"0.1001","P10 2 D7":"0.0944","P10 3 D7":"0.0697","A10 1 D7":"0.0184","A10 2 D7":"0.0262","A10 3 D7":"0.0255","G10 1 D7":"0.0454","G10 2 D7":"0.0441","G10 3 D7":"0.0465","P25 1 D7":"0.0883","P25 2 D7":"0.0922","P25 3 D7":"0.1014","A25 1 D7":"0.0197","A25 2 D7":"0.0139","A25 3 D7":"0.0186","G25 1 D7":"0.0276","G25 2 D7":"0.0266","G25 3 D7":"0.0461","P50 1 D7":"0.0775","P50 2 D7":"0.0705","P50 3 D7":"0.0676","A50 1 D7":"0.0144","A50 2 D7":"0.0159","A50 3 D7":"0.0166","G50 1 D7":"0.0176","G50 2 D7":"0.0527","G50 3 D7":"0.0017","Unsupplemented Control 1 D7":"0.0688","Unsupplemented Control 2 D7":"0.0767","Unsupplemented Control 3 D7":"0.0571","Negative Control 1 D7":"0","Negative Control 2 D7":"0","Negative Control 3 D7":"0"},{"Metabolite":"Glucose","Sterile Saliva":"0","P10 1 D4":"0","P10 2 D4":"0","P10 3 D4":"0","A10 1 D4":"0","A10 2 D4":"0","A10 3 D4":"0","G10 1 D4":"0","G10 2 D4":"0","G10 3 D4":"0","P25 1 D4":"0","P25 2 D4":"0","P25 3 D4":"0","A25 1 D4":"0","A25 2 D4":"0","A25 3 D4":"0","G25 1 D4":"4.8326","G25 2 D4":"5.3765","G25 3 D4":"4.8441","P50 1 D4":"0","P50 2 D4":"0","P50 3 D4":"0","A50 1 D4":"0","A50 2 D4":"0","A50 3 D4":"0","G50 1 D4":"11.6622","G50 2 D4":"13.972","G50 3 D4":"11.4724","Unsupplemented Control 1 D4":"0","Unsupplemented Control 2 D4":"0","Unsupplemented Control 3 D4":"0","Negative Control 1 D4":"0","Negative Control 2 D4":"0","Negative Control 3 D4":"0","P10 1 D7":"0","P10 2 D7":"0","P10 3 D7":"0","A10 1 D7":"0","A10 2 D7":"0","A10 3 D7":"0","G10 1 D7":"0","G10 2 D7":"0","G10 3 D7":"0","P25 1 D7":"0","P25 2 D7":"0","P25 3 D7":"0","A25 1 D7":"0","A25 2 D7":"0","A25 3 D7":"0","G25 1 D7":"6.2758","G25 2 D7":"6.0624","G25 3 D7":"4.4247","P50 1 D7":"0","P50 2 D7":"0","P50 3 D7":"0","A50 1 D7":"0","A50 2 D7":"0","A50 3 D7":"0","G50 1 D7":"13.6535","G50 2 D7":"13.4713","G50 3 D7":"19.3396","Unsupplemented Control 1 D7":"0","Unsupplemented Control 2 D7":"0","Unsupplemented Control 3 D7":"0","Negative Control 1 D7":"0","Negative Control 2 D7":"0","Negative Control 3 D7":"0"},{"Metabolite":"Tyrosine","Sterile Saliva":"0.0249","P10 1 D4":"0","P10 2 D4":"0","P10 3 D4":"0","A10 1 D4":"0","A10 2 D4":"0","A10 3 D4":"0","G10 1 D4":"0","G10 2 D4":"0.0607","G10 3 D4":"0.0356","P25 1 D4":"0","P25 2 D4":"0","P25 3 D4":"0","A25 1 D4":"0.0633","A25 2 D4":"0.0494","A25 3 D4":"0.04","G25 1 D4":"0.0464","G25 2 D4":"0.0515","G25 3 D4":"0.0421","P50 1 D4":"0","P50 2 D4":"0","P50 3 D4":"0","A50 1 D4":"0.0445","A50 2 D4":"0.0379","A50 3 D4":"0.0479","G50 1 D4":"0.0435","G50 2 D4":"0.0467","G50 3 D4":"0.0435","Unsupplemented Control 1 D4":"0","Unsupplemented Control 2 D4":"0.0281","Unsupplemented Control 3 D4":"0.0098","Negative Control 1 D4":"0","Negative Control 2 D4":"0","Negative Control 3 D4":"0.0136","P10 1 D7":"0","P10 2 D7":"0","P10 3 D7":"0","A10 1 D7":"0","A10 2 D7":"0","A10 3 D7":"0","G10 1 D7":"0.0737","G10 2 D7":"0","G10 3 D7":"0.052","P25 1 D7":"0","P25 2 D7":"0","P25 3 D7":"0","A25 1 D7":"0.0719","A25 2 D7":"0.0439","A25 3 D7":"0.0504","G25 1 D7":"0.049","G25 2 D7":"0.0468","G25 3 D7":"0.0496","P50 1 D7":"0","P50 2 D7":"0","P50 3 D7":"0","A50 1 D7":"0.0388","A50 2 D7":"0.0516","A50 3 D7":"0.0413","G50 1 D7":"0.0491","G50 2 D7":"0.0446","G50 3 D7":"0.0134","Unsupplemented Control 1 D7":"0","Unsupplemented Control 2 D7":"0","Unsupplemented Control 3 D7":"0","Negative Control 1 D7":"0","Negative Control 2 D7":"0","Negative Control 3 D7":"0.0156"},{"Metabolite":"Arginine","Sterile Saliva":"0","P10 1 D4":"0","P10 2 D4":"0","P10 3 D4":"0","A10 1 D4":"0","A10 2 D4":"0","A10 3 D4":"0","G10 1 D4":"0","G10 2 D4":"0","G10 3 D4":"0","P25 1 D4":"0","P25 2 D4":"0","P25 3 D4":"0","A25 1 D4":"0","A25 2 D4":"0","A25 3 D4":"0","G25 1 D4":"0","G25 2 D4":"0","G25 3 D4":"0","P50 1 D4":"0","P50 2 D4":"0","P50 3 D4":"0","A50 1 D4":"2.3074","A50 2 D4":"0.0996","A50 3 D4":"3.2188","G50 1 D4":"0","G50 2 D4":"0","G50 3 D4":"0","Unsupplemented Control 1 D4":"0","Unsupplemented Control 2 D4":"0","Unsupplemented Control 3 D4":"0","Negative Control 1 D4":"0","Negative Control 2 D4":"0","Negative Control 3 D4":"0","P10 1 D7":"0","P10 2 D7":"0","P10 3 D7":"0","A10 1 D7":"0","A10 2 D7":"0","A10 3 D7":"0","G10 1 D7":"0","G10 2 D7":"0","G10 3 D7":"0","P25 1 D7":"0","P25 2 D7":"0","P25 3 D7":"0","A25 1 D7":"0.2259","A25 2 D7":"0","A25 3 D7":"0.0664","G25 1 D7":"0","G25 2 D7":"0","G25 3 D7":"0","P50 1 D7":"0","P50 2 D7":"0","P50 3 D7":"0","A50 1 D7":"0.0963","A50 2 D7":"0","A50 3 D7":"0.7123","G50 1 D7":"0","G50 2 D7":"0","G50 3 D7":"0","Unsupplemented Control 1 D7":"0","Unsupplemented Control 2 D7":"0","Unsupplemented Control 3 D7":"0","Negative Control 1 D7":"0","Negative Control 2 D7":"0","Negative Control 3 D7":"0"}]
}

}