{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001507","ANALYSIS_ID":"AN002498","VERSION":"1","CREATED_ON":"October 12, 2020, 12:04 pm"},

"PROJECT":{"PROJECT_TITLE":"Hepatic [U-13C]Lactate tracing and metabolomics in young and old WT and SIRT6 overexpressing mice","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression. Since the liver is the main gluconeogenic organ, we performed here liver metabolomics in young (6 months) versus old (20-24 months) WT and SIRT6-transgenic mice. We used liver tissues of 6h morning-fasted mice, either in naïve state or 15 minutes after intraperitoneal injection of [U-13C]Lactate.","INSTITUTE":"Bar Ilan University","DEPARTMENT":"The Mina & Everard Goodman Faculty of Life Sciences","LABORATORY":"Laboratory of Prof. Haim Cohen","LAST_NAME":"Cohen","FIRST_NAME":"Chaim","ADDRESS":"Ramat Gan, Ramat Gan, Israel, 5290002, Israel","EMAIL":"Haim.Cohen@biu.ac.il","PHONE":"+97235318383","FUNDING_SOURCE":"Israel Science Foundation (621/13 and 777/16), I-Core foundation (1775/12) and (41/11) and Laura and Isaac Perlmutter Foundation","CONTRIBUTORS":"Asael Roichman, Ifat Abramovich, Eyal Gottlieb, Haim Yosef Cohen"},

"STUDY":{"STUDY_TITLE":"Hepatic [U-13C]Lactate tracing and metabolomics in young and old WT and SIRT6 overexpressing mice","STUDY_SUMMARY":"Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression. Since the liver is the main gluconeogenic organ, we performed here liver metabolomics in young (6 months) versus old (20-24 months) WT and SIRT6-transgenic mice. We used liver tissues of 6h morning-fasted mice, either in naïve state or 15 minutes after intraperitoneal injection of 1mg/kg [U-13C]Lactate.","INSTITUTE":"Bar Ilan University","DEPARTMENT":"The Mina & Everard Goodman Faculty of Life Sciences","LABORATORY":"Laboratory of Prof. Haim Cohen","LAST_NAME":"Cohen","FIRST_NAME":"Chaim","ADDRESS":"Bar Ilan University, Ramat Gan, Israel, 5290002","EMAIL":"Haim.Cohen@biu.ac.il","PHONE":"+97235318383","NUM_GROUPS":"8","TOTAL_SUBJECTS":"40"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6JOlaHsd","AGE_OR_AGE_RANGE":"6 months (young) and 20-24 months (old)","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"Envigo","ANIMAL_HOUSING":"Animal rooms were maintained at 21°C-24°C and 35%-75% relative humidity","ANIMAL_LIGHT_CYCLE":"12/12-h (7am to 7pm) dark-light cycle","ANIMAL_FEED":"Altromin 1324 (chow)","ANIMAL_WATER":"Ad lib"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"1",
"Sample ID":"01_Young_Liver_1a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"01_Young_Liver_1a"}
},
{
"Subject ID":"1",
"Sample ID":"02_Young_Liver_1b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"02_Young_Liver_1b"}
},
{
"Subject ID":"1",
"Sample ID":"03_Young_Liver_1c",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"03_Young_Liver_1c"}
},
{
"Subject ID":"2",
"Sample ID":"05_Young_Liver_2a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"05_Young_Liver_2a"}
},
{
"Subject ID":"2",
"Sample ID":"06_Young_Liver_2b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"06_Young_Liver_2b"}
},
{
"Subject ID":"2",
"Sample ID":"07_Young_Liver_2c",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"07_Young_Liver_2c"}
},
{
"Subject ID":"3",
"Sample ID":"09_Young_Liver_3a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"09_Young_Liver_3a"}
},
{
"Subject ID":"3",
"Sample ID":"10_Young_Liver_3b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"10_Young_Liver_3b"}
},
{
"Subject ID":"3",
"Sample ID":"11_Young_Liver_3c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"11_Young_Liver_3c"}
},
{
"Subject ID":"4",
"Sample ID":"13_Young_Liver_4a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"13_Young_Liver_4a"}
},
{
"Subject ID":"4",
"Sample ID":"14_Young_Liver_4b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"14_Young_Liver_4b"}
},
{
"Subject ID":"4",
"Sample ID":"15_Young_Liver_4c",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"15_Young_Liver_4c"}
},
{
"Subject ID":"5",
"Sample ID":"17_Young_Liver_5a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"17_Young_Liver_5a"}
},
{
"Subject ID":"5",
"Sample ID":"18_Young_Liver_5b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"18_Young_Liver_5b"}
},
{
"Subject ID":"5",
"Sample ID":"19_Young_Liver_5c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"19_Young_Liver_5c"}
},
{
"Subject ID":"6",
"Sample ID":"21_Young_Liver_6a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"21_Young_Liver_6a"}
},
{
"Subject ID":"6",
"Sample ID":"22_Young_Liver_6b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"22_Young_Liver_6b"}
},
{
"Subject ID":"6",
"Sample ID":"23_Young_Liver_6c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"23_Young_Liver_6c"}
},
{
"Subject ID":"7",
"Sample ID":"25_Young_Liver_7a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"25_Young_Liver_7a"}
},
{
"Subject ID":"7",
"Sample ID":"26_Young_Liver_7b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"26_Young_Liver_7b"}
},
{
"Subject ID":"7",
"Sample ID":"27_Young_Liver_7c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"27_Young_Liver_7c"}
},
{
"Subject ID":"8",
"Sample ID":"29_Young_Liver_8a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"29_Young_Liver_8a"}
},
{
"Subject ID":"8",
"Sample ID":"30_Young_Liver_8b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"30_Young_Liver_8b"}
},
{
"Subject ID":"8",
"Sample ID":"31_Young_Liver_8c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"31_Young_Liver_8c"}
},
{
"Subject ID":"9",
"Sample ID":"33_Young_Liver_9a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"33_Young_Liver_9a"}
},
{
"Subject ID":"9",
"Sample ID":"34_Young_Liver_9b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"34_Young_Liver_9b"}
},
{
"Subject ID":"10",
"Sample ID":"37_Young_Liver_10a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"37_Young_Liver_10a"}
},
{
"Subject ID":"10",
"Sample ID":"38_Young_Liver_10b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"38_Young_Liver_10b"}
},
{
"Subject ID":"10",
"Sample ID":"39_Young_Liver_10c",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"39_Young_Liver_10c"}
},
{
"Subject ID":"11",
"Sample ID":"41_Young_Liver_11a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"41_Young_Liver_11a"}
},
{
"Subject ID":"11",
"Sample ID":"42_Young_Liver_11b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"42_Young_Liver_11b"}
},
{
"Subject ID":"11",
"Sample ID":"43_Young_Liver_11c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"43_Young_Liver_11c"}
},
{
"Subject ID":"12",
"Sample ID":"45_Young_Liver_12a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"45_Young_Liver_12a"}
},
{
"Subject ID":"12",
"Sample ID":"46_Young_Liver_12b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"46_Young_Liver_12b"}
},
{
"Subject ID":"12",
"Sample ID":"47_Young_Liver_12c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"47_Young_Liver_12c"}
},
{
"Subject ID":"13",
"Sample ID":"49_Young_Liver_13a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"49_Young_Liver_13a"}
},
{
"Subject ID":"13",
"Sample ID":"50_Young_Liver_13b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"50_Young_Liver_13b"}
},
{
"Subject ID":"13",
"Sample ID":"51_Young_Liver_13c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"51_Young_Liver_13c"}
},
{
"Subject ID":"14",
"Sample ID":"53_Young_Liver_14a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"53_Young_Liver_14a"}
},
{
"Subject ID":"14",
"Sample ID":"54_Young_Liver_14b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"54_Young_Liver_14b"}
},
{
"Subject ID":"14",
"Sample ID":"55_Young_Liver_14c",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"55_Young_Liver_14c"}
},
{
"Subject ID":"15",
"Sample ID":"57_Young_Liver_15a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"57_Young_Liver_15a"}
},
{
"Subject ID":"15",
"Sample ID":"58_Young_Liver_15b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"58_Young_Liver_15b"}
},
{
"Subject ID":"15",
"Sample ID":"59_Young_Liver_15c",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"59_Young_Liver_15c"}
},
{
"Subject ID":"16",
"Sample ID":"61_Young_Liver_16a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"61_Young_Liver_16a"}
},
{
"Subject ID":"16",
"Sample ID":"62_Young_Liver_16b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"62_Young_Liver_16b"}
},
{
"Subject ID":"16",
"Sample ID":"63_Young_Liver_16c",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"63_Young_Liver_16c"}
},
{
"Subject ID":"17",
"Sample ID":"65_Young_Liver_17a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"65_Young_Liver_17a"}
},
{
"Subject ID":"17",
"Sample ID":"66_Young_Liver_17b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"66_Young_Liver_17b"}
},
{
"Subject ID":"17",
"Sample ID":"67_Young_Liver_17c",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"67_Young_Liver_17c"}
},
{
"Subject ID":"18",
"Sample ID":"69_Young_Liver_18a",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"69_Young_Liver_18a"}
},
{
"Subject ID":"18",
"Sample ID":"70_Young_Liver_18b",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"70_Young_Liver_18b"}
},
{
"Subject ID":"18",
"Sample ID":"71_Young_Liver_18c",
"Factors":{"Age":"Young","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"71_Young_Liver_18c"}
},
{
"Subject ID":"19",
"Sample ID":"73_Young_Liver_19a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"73_Young_Liver_19a"}
},
{
"Subject ID":"19",
"Sample ID":"74_Young_Liver_19b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"74_Young_Liver_19b"}
},
{
"Subject ID":"19",
"Sample ID":"75_Young_Liver_19c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"75_Young_Liver_19c"}
},
{
"Subject ID":"20",
"Sample ID":"77_Young_Liver_20a",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"77_Young_Liver_20a"}
},
{
"Subject ID":"20",
"Sample ID":"78_Young_Liver_20b",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"78_Young_Liver_20b"}
},
{
"Subject ID":"20",
"Sample ID":"79_Young_Liver_20c",
"Factors":{"Age":"Young","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"79_Young_Liver_20c"}
},
{
"Subject ID":"21",
"Sample ID":"81_Old_Liver_21a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"81_Old_Liver_21a"}
},
{
"Subject ID":"21",
"Sample ID":"82_Old_Liver_21b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"82_Old_Liver_21b"}
},
{
"Subject ID":"21",
"Sample ID":"83_Old_Liver_21c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"83_Old_Liver_21c"}
},
{
"Subject ID":"22",
"Sample ID":"84_Old_Liver_22a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"84_Old_Liver_22a"}
},
{
"Subject ID":"22",
"Sample ID":"85_Old_Liver_22b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"85_Old_Liver_22b"}
},
{
"Subject ID":"22",
"Sample ID":"86_Old_Liver_22c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"86_Old_Liver_22c"}
},
{
"Subject ID":"23",
"Sample ID":"87_Old_Liver_23a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"87_Old_Liver_23a"}
},
{
"Subject ID":"23",
"Sample ID":"88_Old_Liver_23b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"88_Old_Liver_23b"}
},
{
"Subject ID":"23",
"Sample ID":"89_Old_Liver_23c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"89_Old_Liver_23c"}
},
{
"Subject ID":"24",
"Sample ID":"90_Old_Liver_24a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"90_Old_Liver_24a"}
},
{
"Subject ID":"24",
"Sample ID":"91_Old_Liver_24b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"91_Old_Liver_24b"}
},
{
"Subject ID":"24",
"Sample ID":"92_Old_Liver_24c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"92_Old_Liver_24c"}
},
{
"Subject ID":"25",
"Sample ID":"93_Old_Liver_25a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"93_Old_Liver_25a"}
},
{
"Subject ID":"25",
"Sample ID":"94_Old_Liver_25b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"94_Old_Liver_25b"}
},
{
"Subject ID":"25",
"Sample ID":"95_Old_Liver_25c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"95_Old_Liver_25c"}
},
{
"Subject ID":"26",
"Sample ID":"96_Old_Liver_26a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"96_Old_Liver_26a"}
},
{
"Subject ID":"26",
"Sample ID":"97_Old_Liver_26b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"97_Old_Liver_26b"}
},
{
"Subject ID":"26",
"Sample ID":"98_Old_Liver_26c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"98_Old_Liver_26c"}
},
{
"Subject ID":"27",
"Sample ID":"99_Old_Liver_27a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"99_Old_Liver_27a"}
},
{
"Subject ID":"27",
"Sample ID":"100_Old_Liver_27b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"100_Old_Liver_27b"}
},
{
"Subject ID":"27",
"Sample ID":"101_Old_Liver_27c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"101_Old_Liver_27c"}
},
{
"Subject ID":"28",
"Sample ID":"102_Old_Liver_28a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"102_Old_Liver_28a"}
},
{
"Subject ID":"28",
"Sample ID":"103_Old_Liver_28b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"103_Old_Liver_28b"}
},
{
"Subject ID":"28",
"Sample ID":"104_Old_Liver_28c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"104_Old_Liver_28c"}
},
{
"Subject ID":"29",
"Sample ID":"105_Old_Liver_29a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"105_Old_Liver_29a"}
},
{
"Subject ID":"29",
"Sample ID":"106_Old_Liver_29b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"106_Old_Liver_29b"}
},
{
"Subject ID":"29",
"Sample ID":"107_Old_Liver_29c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"107_Old_Liver_29c"}
},
{
"Subject ID":"30",
"Sample ID":"108_Old_Liver_30a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"108_Old_Liver_30a"}
},
{
"Subject ID":"30",
"Sample ID":"109_Old_Liver_30b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"109_Old_Liver_30b"}
},
{
"Subject ID":"30",
"Sample ID":"110_Old_Liver_30c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"N/A"},
"Additional sample data":{"RAW_FILE_NAME":"110_Old_Liver_30c"}
},
{
"Subject ID":"31",
"Sample ID":"111_Old_Liver_31a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"111_Old_Liver_31a"}
},
{
"Subject ID":"31",
"Sample ID":"112_Old_Liver_31b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"112_Old_Liver_31b"}
},
{
"Subject ID":"31",
"Sample ID":"113_Old_Liver_31c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"113_Old_Liver_31c"}
},
{
"Subject ID":"32",
"Sample ID":"114_Old_Liver_32a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"114_Old_Liver_32a"}
},
{
"Subject ID":"32",
"Sample ID":"115_Old_Liver_32b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"115_Old_Liver_32b"}
},
{
"Subject ID":"32",
"Sample ID":"116_Old_Liver_32c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"116_Old_Liver_32c"}
},
{
"Subject ID":"33",
"Sample ID":"117_Old_Liver_33a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"117_Old_Liver_33a"}
},
{
"Subject ID":"33",
"Sample ID":"118_Old_Liver_33b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"118_Old_Liver_33b"}
},
{
"Subject ID":"33",
"Sample ID":"119_Old_Liver_33c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"119_Old_Liver_33c"}
},
{
"Subject ID":"34",
"Sample ID":"120_Old_Liver_34a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"120_Old_Liver_34a"}
},
{
"Subject ID":"34",
"Sample ID":"121_Old_Liver_34b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"121_Old_Liver_34b"}
},
{
"Subject ID":"34",
"Sample ID":"122_Old_Liver_34c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"122_Old_Liver_34c"}
},
{
"Subject ID":"35",
"Sample ID":"123_Old_Liver_35a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"123_Old_Liver_35a"}
},
{
"Subject ID":"35",
"Sample ID":"124_Old_Liver_35b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"124_Old_Liver_35b"}
},
{
"Subject ID":"35",
"Sample ID":"125_Old_Liver_35c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"125_Old_Liver_35c"}
},
{
"Subject ID":"36",
"Sample ID":"126_Old_Liver_36a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"126_Old_Liver_36a"}
},
{
"Subject ID":"36",
"Sample ID":"127_Old_Liver_36b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"127_Old_Liver_36b"}
},
{
"Subject ID":"36",
"Sample ID":"128_Old_Liver_36c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"128_Old_Liver_36c"}
},
{
"Subject ID":"37",
"Sample ID":"129_Old_Liver_37a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"129_Old_Liver_37a"}
},
{
"Subject ID":"37",
"Sample ID":"130_Old_Liver_37b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"130_Old_Liver_37b"}
},
{
"Subject ID":"37",
"Sample ID":"131_Old_Liver_37c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"131_Old_Liver_37c"}
},
{
"Subject ID":"38",
"Sample ID":"132_Old_Liver_38a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"132_Old_Liver_38a"}
},
{
"Subject ID":"38",
"Sample ID":"133_Old_Liver_38b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"133_Old_Liver_38b"}
},
{
"Subject ID":"38",
"Sample ID":"134_Old_Liver_38c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"134_Old_Liver_38c"}
},
{
"Subject ID":"39",
"Sample ID":"135_Old_Liver_39a",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"135_Old_Liver_39a"}
},
{
"Subject ID":"39",
"Sample ID":"136_Old_Liver_39b",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"136_Old_Liver_39b"}
},
{
"Subject ID":"39",
"Sample ID":"137_Old_Liver_39c",
"Factors":{"Age":"Old","Genotype":"SIRT6-tg","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"137_Old_Liver_39c"}
},
{
"Subject ID":"40",
"Sample ID":"138_Old_Liver_40a",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"138_Old_Liver_40a"}
},
{
"Subject ID":"40",
"Sample ID":"139_Old_Liver_40b",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"139_Old_Liver_40b"}
},
{
"Subject ID":"40",
"Sample ID":"140_Old_Liver_40c",
"Factors":{"Age":"Old","Genotype":"Wild-type","Treatment":"[U-13C]Lactate"},
"Additional sample data":{"RAW_FILE_NAME":"140_Old_Liver_40c"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mice were euthanized by cervical dislocation, left liver lobe was collected as fast as possible (<30 sec) and immediately flash-frozen in liquid nitrogen. Tissues were stored at -80C until metabolite extraction.","SAMPLE_TYPE":"Liver","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"All mice were fasted for 6h (8AM-2PM). Before sacrifice, tail blood was collected from all mice for less than 2 minutes. The untreated group were sacrificed at this point. For 13C-lactate treatment, 6h fasted mice were intraperitoneally injected with 1mg/kg [U-13C]Lactate (CIL) and sacrificed 15 minutes after injection.","TREATMENT":"[U-13C]Lactate injection","TREATMENT_ROUTE":"Intraperitoneal injection","TREATMENT_DOSE":"1mg/kg","TREATMENT_DOSEVOLUME":"300ul for 30gr mouse","ANIMAL_ANESTHESIA":"N/A","ANIMAL_FASTING":"6h morning fast","ANIMAL_ENDP_EUTHANASIA":"Cervical dislocation","TREATMENT_COMPOUND":"[U-13C]Lactate"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"To maximize accuracy and to take into account tissue inhomogeneity, metabolites were extracted from 3 separate liver pieces for each mouse, and the average metabolite value of the 3 technical replicates was calculated at analysis. In detail, frozen liver pieces weighting ~30mg were transferred into soft tissue homogenizing CK 14 tubes containing 1.4 mm ceramic beads (Bertin corp.) prefilled with 1 ml of cold (-20°C) metabolites extraction solvent (Methanol: Acetonitrile:H2O, 50:30:20) and kept on ice. Samples were homogenized using precellys 24 tissue homogenizer (3X20sec at 6000 rpm, with a 30sec gap between each of the three cycles, Bertin Technologies) cooled to 2°C. Homogenized extracts were centrifuged in the Precellys tubes at 18,000g for 15 minutes at 4°C, supernatants were collected in microcentrifuge tubes and centrifuged again at 18,000g for 10 minutes at 4°C. The supernatants were transferred to glass HPLC vials and kept at -75 °C prior to LC-MS analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"SeQuant ZIC-HILIC (150 x 2.1mm, 5um)","FLOW_RATE":"0.2 ml/min","COLUMN_TEMPERATURE":"45","SOLVENT_A":"20 mM ammonium carbonate adjusted to pH 9.2 with 0.1% of 25% ammonium hydroxide","SOLVENT_B":"acetonitrile"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Prof. Eyal Gottlieb","OPERATOR_NAME":"Ifat Abramovich"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"polarity switching mode to enable both positive and negative ions was used"},

"MS_METABOLITE_DATA":{
"Units":"Peak Area",

"Data":[{"Metabolite":"3-hydroxybutyric 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+6"},{"metabolite_name":"GSH +7"},{"metabolite_name":"GSH +8"},{"metabolite_name":"GSH +9"},{"metabolite_name":"Hydroxyproline"},{"metabolite_name":"Hydroxyproline + 1"},{"metabolite_name":"Hydroxyproline + 2"},{"metabolite_name":"Hydroxyproline + 3"},{"metabolite_name":"Sedoheptulose-7-phosphate (D)"},{"metabolite_name":"Sedoheptulose-7-phosphate (D) + 1"},{"metabolite_name":"Sedoheptulose-7-phosphate (D) + 2"},{"metabolite_name":"Sedoheptulose-7-phosphate (D) + 3"},{"metabolite_name":"Sedoheptulose-7-phosphate (D) + 4"},{"metabolite_name":"Sedoheptulose-7-phosphate (D) + 5"},{"metabolite_name":"Sedoheptulose-7-phosphate (D) + 6"},{"metabolite_name":"Sedoheptulose-7-phosphate (D) + 7"},{"metabolite_name":"Allantoin"},{"metabolite_name":"aminoadipate"},{"metabolite_name":"Beta-hydroxyvaleric Acid"},{"metabolite_name":"Creatinine"},{"metabolite_name":"dihydroxyacetone phosphate (DHAP)"},{"metabolite_name":"Glycerol"},{"metabolite_name":"Serotonin (nv)"},{"metabolite_name":"Valine"},{"metabolite_name":"beta Alanine"},{"metabolite_name":"Myristoyl-carnitine"},{"metabolite_name":"Palmitoyl-carnitine"},{"metabolite_name":"GSSG"},{"metabolite_name":"GSSG +1"},{"metabolite_name":"GSSG +2"},{"metabolite_name":"GSSG +3"},{"metabolite_name":"GSSG +4"},{"metabolite_name":"GSSG +5"},{"metabolite_name":"GSSG +6"},{"metabolite_name":"GSSG +7"},{"metabolite_name":"GSSG +8"}]
}

}