{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001514","ANALYSIS_ID":"AN002508","VERSION":"1","CREATED_ON":"October 30, 2020, 11:03 am"},

"PROJECT":{"PROJECT_TITLE":"Community metabolomes reflect taxon-specific fingerprints of phytoplankton in the ocean","PROJECT_TYPE":"Marine Metabolomics","PROJECT_SUMMARY":"Phytoplankton transform inorganic carbon into thousands of biomolecules, including polar metabolites that represent an important pool of labile fixed carbon, nitrogen, and sulfur. Metabolite production is not identical among phytoplankton, and the flux of these molecules through the microbial loop depends on compound-specific bioavailability to a wider microbial community. Yet relatively little is known about the diversity or concentration of polar metabolites within marine plankton. Here we evaluate 313 metabolites in 21 phytoplankton species and in natural marine particles across environmental gradients to show that bulk community metabolomes reflect the phytoplankton community on a chemical level.","INSTITUTE":"University of Washington","DEPARTMENT":"Oceanography","LABORATORY":"Ingalls Lab","LAST_NAME":"Heal","FIRST_NAME":"Katherine","ADDRESS":"1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA, 98195, USA","EMAIL":"kheal@uw.edu","PHONE":"612-616-4840"},

"STUDY":{"STUDY_TITLE":"Community metabolomes reflect taxon-specific fingerprints of phytoplankton in the ocean","STUDY_TYPE":"Metabolomic survey of 21 phytoplankton species","STUDY_SUMMARY":"Phytoplankton transform inorganic carbon into thousands of biomolecules, including polar metabolites that represent an important pool of labile fixed carbon, nitrogen, and sulfur. Metabolite production is not identical among phytoplankton, and the flux of these molecules through the microbial loop depends on compound-specific bioavailability to a wider microbial community. Yet relatively little is known about the diversity or concentration of polar metabolites within marine plankton. Here we evaluate 313 metabolites in 21 phytoplankton species and in natural marine particles across environmental gradients to show that bulk community metabolomes reflect the phytoplankton community on a chemical level.","INSTITUTE":"University of Washington","DEPARTMENT":"Oceanography","LABORATORY":"Ingalls Lab","LAST_NAME":"Heal","FIRST_NAME":"Katherine","ADDRESS":"1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA, 98195, USA","EMAIL":"kheal@uw.edu","PHONE":"612-616-4840"},

"SUBJECT":{"SUBJECT_TYPE":"Other","SUBJECT_SPECIES":"Marine Plankton","GENDER":"Not applicable"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Nmar_1",
"Factors":{"Species":"Nitrosopumilus maritimus","Strain":"SCM1","Broad taxon":"Archaea"},
"Additional sample data":{"Biovolume filtered_uL":"0.11362","nmol C filtered":"4116.666667","Short ID":"Nmar","RAW_FILE_NAME":"180130_Exp_1 180130_Smp_Exp_1 180131_Smp_Exp_1"}
},
{
"Subject ID":"-",
"Sample ID":"Nmar_2",
"Factors":{"Species":"Nitrosopumilus maritimus","Strain":"SCM1","Broad taxon":"Archaea"},
"Additional sample data":{"Biovolume filtered_uL":"0.10396","nmol C filtered":"3766.666667","Short ID":"Nmar","RAW_FILE_NAME":"180130_Exp_2 180130_Smp_Exp_2 180131_Smp_Exp_2"}
},
{
"Subject ID":"-",
"Sample ID":"Nmar_3",
"Factors":{"Species":"Nitrosopumilus maritimus","Strain":"SCM1","Broad taxon":"Archaea"},
"Additional sample data":{"Biovolume filtered_uL":"0.09982","nmol C filtered":"3616.666667","Short ID":"Nmar","RAW_FILE_NAME":"180130_Exp_3 180130_Smp_Exp_3 180131_Smp_Exp_3"}
},
{
"Subject ID":"-",
"Sample ID":"8501_B",
"Factors":{"Species":"Crocosphaera watsonii","Strain":"8501","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"3.711258","nmol C filtered":"15773","Short ID":"8501","RAW_FILE_NAME":"161114_Smp_8501_B 161114_Smp_8501_B 161118_Smp_8501_B"}
},
{
"Subject ID":"-",
"Sample ID":"8501_C",
"Factors":{"Species":"Crocosphaera watsonii","Strain":"8501","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"5.351031818","nmol C filtered":"22742","Short ID":"8501","RAW_FILE_NAME":"161114_Smp_8501_C 161114_Smp_8501_C 161118_Smp_8501_C"}
},
{
"Subject ID":"-",
"Sample ID":"8501_D",
"Factors":{"Species":"Crocosphaera watsonii","Strain":"8501","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"3.515528571","nmol C filtered":"14941","Short ID":"8501","RAW_FILE_NAME":"161114_Smp_8501.D 161114_Smp_8501.D 161118_Smp_8501_D"}
},
{
"Subject ID":"-",
"Sample ID":"1314P_A",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"1314","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.3185","nmol C filtered":"5898.2295","Short ID":"1314P","RAW_FILE_NAME":"170821_Smp_1314P_A 170821_Smp_1314P_A 170828_Smp_1314P_A"}
},
{
"Subject ID":"-",
"Sample ID":"1314P_B",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"1314","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.34858","nmol C filtered":"6455.239233","Short ID":"1314P","RAW_FILE_NAME":"170821_Smp_1314P_B 170821_Smp_1314P_B 170828_Smp_1314P_B"}
},
{
"Subject ID":"-",
"Sample ID":"1314P_C",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"1314","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.31701","nmol C filtered":"5870.54047","Short ID":"1314P","RAW_FILE_NAME":"170821_Smp_1314P_C 170821_Smp_1314P_C 170828_Smp_1314P_C"}
},
{
"Subject ID":"-",
"Sample ID":"As9601_A",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"AS9601","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.452556309","nmol C filtered":"10475.84048","Short ID":"As9601","RAW_FILE_NAME":"170821_Smp_As9601_A 170821_Smp_As9601_A 170828_Smp_As9601_A"}
},
{
"Subject ID":"-",
"Sample ID":"As9601_B",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"AS9601","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.487486222","nmol C filtered":"11284.40329","Short ID":"As9601","RAW_FILE_NAME":"170821_Smp_As9601_B 170821_Smp_As9601_B 170828_Smp_As9601_B"}
},
{
"Subject ID":"-",
"Sample ID":"As9601_C",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"AS9601","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.467084563","nmol C filtered":"10812.14267","Short ID":"As9601","RAW_FILE_NAME":"170821_Smp_As9601_C 170821_Smp_As9601_C 170828_Smp_As9601_C"}
},
{
"Subject ID":"-",
"Sample ID":"MED4_D",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"MED4","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.390883343","nmol C filtered":"5428.935323","Short ID":"MED4","RAW_FILE_NAME":"161114_Smp_MED4.D 161114_Smp_MED4.D 161118_Smp_MED4_D"}
},
{
"Subject ID":"-",
"Sample ID":"MED4_E",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"MED4","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.346499459","nmol C filtered":"4812.492493","Short ID":"MED4","RAW_FILE_NAME":"161114_Smp_MED4_E 161114_Smp_MED4_E 161118_Smp_MED4_E"}
},
{
"Subject ID":"-",
"Sample ID":"MED4_F",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"MED4","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.395070736","nmol C filtered":"5487.093558","Short ID":"MED4","RAW_FILE_NAME":"161114_Smp_MED4_F 161114_Smp_MED4_F 161118_Smp_MED4_F"}
},
{
"Subject ID":"-",
"Sample ID":"Nat_A",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"NATL2A","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.062888292","nmol C filtered":"1886.648745","Short ID":"Nat","RAW_FILE_NAME":"170821_Smp_Nat_A 170821_Smp_Nat_A 170828_Smp_Nat_A"}
},
{
"Subject ID":"-",
"Sample ID":"Nat_B",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"NATL2A","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.054405","nmol C filtered":"1632.15","Short ID":"Nat","RAW_FILE_NAME":"170821_Smp_Nat_B 170821_Smp_Nat_B 170828_Smp_Nat_B"}
},
{
"Subject ID":"-",
"Sample ID":"Nat_C",
"Factors":{"Species":"Prochlorococcus marinus","Strain":"NATL2A","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.054925488","nmol C filtered":"1647.764655","Short ID":"Nat","RAW_FILE_NAME":"170821_Smp_Nat_C 170821_Smp_Nat_C 170828_Smp_Nat_C"}
},
{
"Subject ID":"-",
"Sample ID":"7803_B",
"Factors":{"Species":"Synechococcus sp.","Strain":"7803","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"2.20472","nmol C filtered":"33039.88864","Short ID":"7803","RAW_FILE_NAME":"161114_Smp_7803_B 161114_Smp_7803_B 161118_Smp_7803_B"}
},
{
"Subject ID":"-",
"Sample ID":"7803_F",
"Factors":{"Species":"Synechococcus sp.","Strain":"7803","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"2.18928","nmol C filtered":"35225.87859","Short ID":"7803","RAW_FILE_NAME":"161114_Smp_7803_F 161114_Smp_7803_F 161118_Smp_7803_F"}
},
{
"Subject ID":"-",
"Sample ID":"8102_A",
"Factors":{"Species":"Synechococcus sp.","Strain":"8102","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.708","nmol C filtered":"47768.85007","Short ID":"8102","RAW_FILE_NAME":"161114_Smp_8102_A 161114_Smp_8102_A 161118_Smp_8102_A"}
},
{
"Subject ID":"-",
"Sample ID":"8102_F",
"Factors":{"Species":"Synechococcus sp.","Strain":"8102","Broad taxon":"Cyanobacteria"},
"Additional sample data":{"Biovolume filtered_uL":"0.75484","nmol C filtered":"47434.36605","Short ID":"8102","RAW_FILE_NAME":"161114_Smp_8102_F 161114_Smp_8102_F 161118_Smp_8102_F"}
},
{
"Subject ID":"-",
"Sample ID":"Cy_A",
"Factors":{"Species":"Cyclotella meneghiniana","Strain":"338","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"7.0207956","nmol C filtered":"66158.68808","Short ID":"Cy","RAW_FILE_NAME":"161107_Smp_Cy_A 161107_Smp_Cy_A 161110_Smp_Cy_A"}
},
{
"Subject ID":"-",
"Sample ID":"Cy_B",
"Factors":{"Species":"Cyclotella meneghiniana","Strain":"338","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"2.285525455","nmol C filtered":"21537.06991","Short ID":"Cy","RAW_FILE_NAME":"161107_Smp_Cy_B 161107_Smp_Cy_B 161110_Smp_Cy_B"}
},
{
"Subject ID":"-",
"Sample ID":"Cy_C",
"Factors":{"Species":"Cyclotella meneghiniana","Strain":"338","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"4.9629762","nmol C filtered":"46767.34847","Short ID":"Cy","RAW_FILE_NAME":"161107_Smp_Cy_C 161107_Smp_Cy_C 161110_Smp_Cy_C"}
},
{
"Subject ID":"-",
"Sample ID":"Np_A",
"Factors":{"Species":"Navicula pelliculosa","Strain":"543","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"4.429566","nmol C filtered":"50707.78032","Short ID":"Np","RAW_FILE_NAME":"170213_Smp_Np_A 170213_Smp_Np_A 170216_Smp_Np_A"}
},
{
"Subject ID":"-",
"Sample ID":"Np_C",
"Factors":{"Species":"Navicula pelliculosa","Strain":"543","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"5.07693","nmol C filtered":"58118.52699","Short ID":"Np","RAW_FILE_NAME":"170213_Smp_Np_C 170213_Smp_Np_C 170216_Smp_Np_C"}
},
{
"Subject ID":"-",
"Sample ID":"Np_F",
"Factors":{"Species":"Navicula pelliculosa","Strain":"543","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"5.69769","nmol C filtered":"65224.72243","Short ID":"Np","RAW_FILE_NAME":"170213_Smp_Np_F 170213_Smp_Np_F 170216_Smp_Np_F"}
},
{
"Subject ID":"-",
"Sample ID":"Pt_C",
"Factors":{"Species":"Phaeodactylum tricornutum","Strain":"2561","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"52.49856","nmol C filtered":"405673.6567","Short ID":"Pt","RAW_FILE_NAME":"170213_Smp_Pt_C 170213_Smp_Pt_C 170216_Smp_Pt_C"}
},
{
"Subject ID":"-",
"Sample ID":"Pt_F",
"Factors":{"Species":"Phaeodactylum tricornutum","Strain":"2561","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"55.51368","nmol C filtered":"428972.4816","Short ID":"Pt","RAW_FILE_NAME":"170213_Smp_Pt_F 170213_Smp_Pt_F 170216_Smp_Pt_F"}
},
{
"Subject ID":"-",
"Sample ID":"Pc55x_A",
"Factors":{"Species":"Pseudo-nitzschia pungens","Strain":"Pc55x","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"3.980689655","nmol C filtered":"23670.0076","Short ID":"Pc55x","RAW_FILE_NAME":"170213_Smp_Pc55x_A 170213_Smp_Pc55x_A 170216_Smp_Pc55x_A"}
},
{
"Subject ID":"-",
"Sample ID":"Pc55x_B",
"Factors":{"Species":"Pseudo-nitzschia pungens","Strain":"Pc55x","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"4.154482759","nmol C filtered":"24703.4175","Short ID":"Pc55x","RAW_FILE_NAME":"170213_Smp_Pc55x_B 170213_Smp_Pc55x_B 170216_Smp_Pc55x_B"}
},
{
"Subject ID":"-",
"Sample ID":"Pc55x_C",
"Factors":{"Species":"Pseudo-nitzschia pungens","Strain":"Pc55x","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"2.532631579","nmol C filtered":"15059.55348","Short ID":"Pc55x","RAW_FILE_NAME":"170213_Smp_Pc55x_C 170213_Smp_Pc55x_C 170216_Smp_Pc55x_C"}
},
{
"Subject ID":"-",
"Sample ID":"To_A",
"Factors":{"Species":"Thalassiosira oceanica","Strain":"1005","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"10.10952","nmol C filtered":"94030.34765","Short ID":"To","RAW_FILE_NAME":"161107_Smp_To_A 161107_Smp_To_A 161110_Smp_To_A"}
},
{
"Subject ID":"-",
"Sample ID":"To_B",
"Factors":{"Species":"Thalassiosira oceanica","Strain":"1005","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"13.26875","nmol C filtered":"123414.8313","Short ID":"To","RAW_FILE_NAME":"161107_Smp_To_B 161107_Smp_To_B 161110_Smp_To_B"}
},
{
"Subject ID":"-",
"Sample ID":"To_C",
"Factors":{"Species":"Thalassiosira oceanica","Strain":"1005","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"12.20459","nmol C filtered":"113516.9","Short ID":"To","RAW_FILE_NAME":"161107_Smp_To_C 161107_Smp_To_C 161110_Smp_To_C"}
},
{
"Subject ID":"-",
"Sample ID":"Tp_A",
"Factors":{"Species":"Thalassiosira pseudonana","Strain":"1335","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"4.40222","nmol C filtered":"133534.0977","Short ID":"Tp","RAW_FILE_NAME":"161107_Smp_Tp_A 161107_Smp_Tp_A 161110_Smp_Tp_A"}
},
{
"Subject ID":"-",
"Sample ID":"Tp_E",
"Factors":{"Species":"Thalassiosira pseudonana","Strain":"1335","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"3.13927","nmol C filtered":"95224.584","Short ID":"Tp","RAW_FILE_NAME":"161107_Smp_Tp_E 161107_Smp_Tp_E 161110_Smp_Tp_E"}
},
{
"Subject ID":"-",
"Sample ID":"Tp_F",
"Factors":{"Species":"Thalassiosira pseudonana","Strain":"1335","Broad taxon":"Diatom"},
"Additional sample data":{"Biovolume filtered_uL":"6.36368","nmol C filtered":"193031.5296","Short ID":"Tp","RAW_FILE_NAME":"161107_Smp_Tp_F 161107_Smp_Tp_F 161110_Smp_Tp_F"}
},
{
"Subject ID":"-",
"Sample ID":"1771_A",
"Factors":{"Species":"Alexandrium tamarense","Strain":"1771","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"0.45029728","nmol C filtered":"4416.314582","Short ID":"1771","RAW_FILE_NAME":"170821_Smp_1771_A 170821_Smp_1771_A 170828_Smp_1771_A"}
},
{
"Subject ID":"-",
"Sample ID":"1771_B",
"Factors":{"Species":"Alexandrium tamarense","Strain":"1771","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"0.39780864","nmol C filtered":"3901.529447","Short ID":"1771","RAW_FILE_NAME":"170821_Smp_1771_B 170821_Smp_1771_B 170828_Smp_1771_B"}
},
{
"Subject ID":"-",
"Sample ID":"1771_C",
"Factors":{"Species":"Alexandrium tamarense","Strain":"1771","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"0.23205504","nmol C filtered":"2275.892177","Short ID":"1771","RAW_FILE_NAME":"170821_Smp_1771_C 170821_Smp_1771_C 170828_Smp_1771_C"}
},
{
"Subject ID":"-",
"Sample ID":"1314_A",
"Factors":{"Species":"Amphidinium carterae","Strain":"1314","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"0.56953488","nmol C filtered":"8754.975631","Short ID":"1314","RAW_FILE_NAME":"170821_Smp_1314_A 170821_Smp_1314_A 170828_Smp_1314_A"}
},
{
"Subject ID":"-",
"Sample ID":"1314_B",
"Factors":{"Species":"Amphidinium carterae","Strain":"1314","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"0.5827728","nmol C filtered":"8958.470924","Short ID":"1314","RAW_FILE_NAME":"170821_Smp_1314_B 170821_Smp_1314_B 170828_Smp_1314_B"}
},
{
"Subject ID":"-",
"Sample ID":"1314_C",
"Factors":{"Species":"Amphidinium carterae","Strain":"1314","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"0.66859104","nmol C filtered":"10277.68179","Short ID":"1314","RAW_FILE_NAME":"170821_Smp_1314_C 170821_Smp_1314_C 170828_Smp_1314_C"}
},
{
"Subject ID":"-",
"Sample ID":"449_A",
"Factors":{"Species":"Heterocapsa triquetra","Strain":"449","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"0.33903","nmol C filtered":"4462.579749","Short ID":"449","RAW_FILE_NAME":"170821_Smp_449_A 170821_Smp_449_A 170828_Smp_449_A"}
},
{
"Subject ID":"-",
"Sample ID":"449_B",
"Factors":{"Species":"Heterocapsa triquetra","Strain":"449","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"0.48124","nmol C filtered":"6334.523314","Short ID":"449","RAW_FILE_NAME":"170821_Smp_449_B 170821_Smp_449_B 170828_Smp_449_B"}
},
{
"Subject ID":"-",
"Sample ID":"449_C",
"Factors":{"Species":"Heterocapsa triquetra","Strain":"449","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"0.55139","nmol C filtered":"7257.95976","Short ID":"449","RAW_FILE_NAME":"170821_Smp_449_C 170821_Smp_449_C 170828_Smp_449_C"}
},
{
"Subject ID":"-",
"Sample ID":"2021_A",
"Factors":{"Species":"Lingulodinium polyedra","Strain":"2021","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"2.75917726","nmol C filtered":"26427.31696","Short ID":"2021","RAW_FILE_NAME":"170821_Smp_2021_A 170821_Smp_2021_A 170828_Smp_2021_A"}
},
{
"Subject ID":"-",
"Sample ID":"2021_B",
"Factors":{"Species":"Lingulodinium polyedra","Strain":"2021","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"5.11555492","nmol C filtered":"48996.63145","Short ID":"2021","RAW_FILE_NAME":"170821_Smp_2021_B 170821_Smp_2021_B 170828_Smp_2021_B"}
},
{
"Subject ID":"-",
"Sample ID":"2021_C",
"Factors":{"Species":"Lingulodinium polyedra","Strain":"2021","Broad taxon":"Dinoflagellate"},
"Additional sample data":{"Biovolume filtered_uL":"1.53228256","nmol C filtered":"14676.15636","Short ID":"2021","RAW_FILE_NAME":"170821_Smp_2021_C 170821_Smp_2021_C 170828_Smp_2021_C"}
},
{
"Subject ID":"-",
"Sample ID":"2090_A",
"Factors":{"Species":"Emiliania huxleyi","Strain":"2090","Broad taxon":"Haptophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.29131","nmol C filtered":"3813.921528","Short ID":"2090","RAW_FILE_NAME":"161107_Smp_2090_A 161107_Smp_2090_A 161110_Smp_2090_A"}
},
{
"Subject ID":"-",
"Sample ID":"2090_B",
"Factors":{"Species":"Emiliania huxleyi","Strain":"2090","Broad taxon":"Haptophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.33255","nmol C filtered":"4353.791699","Short ID":"2090","RAW_FILE_NAME":"161107_Smp_2090_B 161107_Smp_2090_B 161110_Smp_2090_B"}
},
{
"Subject ID":"-",
"Sample ID":"2090_C",
"Factors":{"Species":"Emiliania huxleyi","Strain":"2090","Broad taxon":"Haptophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.381324","nmol C filtered":"4992.347815","Short ID":"2090","RAW_FILE_NAME":"161107_Smp_2090_C 161107_Smp_2090_C 161110_Smp_2090_C"}
},
{
"Subject ID":"-",
"Sample ID":"371_A",
"Factors":{"Species":"Emiliania huxleyi","Strain":"371","Broad taxon":"Haptophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.365805","nmol C filtered":"4789.170869","Short ID":"371","RAW_FILE_NAME":"161107_Smp_371_A 161107_Smp_371_A 161110_Smp_371_A"}
},
{
"Subject ID":"-",
"Sample ID":"371_B",
"Factors":{"Species":"Emiliania huxleyi","Strain":"371","Broad taxon":"Haptophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.380920909","nmol C filtered":"4987.070491","Short ID":"371","RAW_FILE_NAME":"161107_Smp_371_B 161107_Smp_371_B 161110_Smp_371_B"}
},
{
"Subject ID":"-",
"Sample ID":"371_C",
"Factors":{"Species":"Emiliania huxleyi","Strain":"371","Broad taxon":"Haptophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.294256364","nmol C filtered":"3852.445988","Short ID":"371","RAW_FILE_NAME":"161107_Smp_371_C 161107_Smp_371_C 161110_Smp_371_C"}
},
{
"Subject ID":"-",
"Sample ID":"1545_A",
"Factors":{"Species":"Micromonas pusilla","Strain":"1545","Broad taxon":"Prasinophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.557926221","nmol C filtered":"65905.03488","Short ID":"1545","RAW_FILE_NAME":"170821_Smp_1545_A 170821_Smp_1545_A 170828_Smp_1545_A"}
},
{
"Subject ID":"-",
"Sample ID":"1545_B",
"Factors":{"Species":"Micromonas pusilla","Strain":"1545","Broad taxon":"Prasinophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.571037924","nmol C filtered":"67453.85477","Short ID":"1545","RAW_FILE_NAME":"170821_Smp_1545_B 170821_Smp_1545_B 170828_Smp_1545_B"}
},
{
"Subject ID":"-",
"Sample ID":"1545_C",
"Factors":{"Species":"Micromonas pusilla","Strain":"1545","Broad taxon":"Prasinophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.536432713","nmol C filtered":"63366.11429","Short ID":"1545","RAW_FILE_NAME":"170821_Smp_1545_C 170821_Smp_1545_C 170828_Smp_1545_C"}
},
{
"Subject ID":"-",
"Sample ID":"3430_A",
"Factors":{"Species":"Ostreococcus lucimarinus","Strain":"3430","Broad taxon":"Prasinophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.169351485","nmol C filtered":"33870","Short ID":"3430","RAW_FILE_NAME":"180227_Smp_3430_A 180227_Smp_3430_A 180302_Smp_3430_A"}
},
{
"Subject ID":"-",
"Sample ID":"3430_B",
"Factors":{"Species":"Ostreococcus lucimarinus","Strain":"3430","Broad taxon":"Prasinophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.172402985","nmol C filtered":"34481","Short ID":"3430","RAW_FILE_NAME":"180227_Smp_3430_B 180227_Smp_3430_B 180302_Smp_3430_B"}
},
{
"Subject ID":"-",
"Sample ID":"3430_C",
"Factors":{"Species":"Ostreococcus lucimarinus","Strain":"3430","Broad taxon":"Prasinophyte"},
"Additional sample data":{"Biovolume filtered_uL":"0.175519802","nmol C filtered":"35104","Short ID":"3430","RAW_FILE_NAME":"180227_Smp_3430_C 180227_Smp_3430_C 180302_Smp_3430_C"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Axenic phytoplankton were cultured in controlled laboratory settings and harvested under exponential growth using a gentle vacuum filtration onto 47 mm Durapore filters (pore size 0.2 µm). Samples were flash frozen in liquid N2 and stored at -80°C until extraction for metabolites.","SAMPLE_TYPE":"Cultured plankton cells","STORAGE_CONDITIONS":"Described in summary"},

"TREATMENT":{"TREATMENT_SUMMARY":"Media, light, and temperature were chosen for optimal growth of each phytoplankton species. In short, axenic phytoplankton were cultured in controlled laboratory settings and harvested under exponential growth using a gentle vacuum filtration onto 47 mm Durapore filters (pore size 0.2 µm). Samples were flash frozen in liquid N2 and stored at -80°C until extraction. In addition to samples, media blanks corresponding to each media type were harvested and served as a matrix blank to each corresponding phytoplankton sample."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Each sample was extracted using a modified Bligh-Dyer extraction. Briefly, filters were cut up and put into 15 mL teflon centrifuge tubes containing a mixture of 100 µm and 400 µm silica beads. Heavy isotope-labeled internal standards were added along with ~2 mL of cold aqueous solvent (50:50 methanol:water) and ~3 mL of cold organic solvent (dichloromethane). The samples were shaken on a FastPrep-24 Homogenizer for 30 seconds and chilled in a -20°C freezer repeatedly for three cycles of bead-beating and a total of 30 minutes of chilling. The organic and aqueous layers were separated by spinning samples in a centrifuge at 4,300 rpm for 2 minutes at 4°C. The aqueous layer was removed to a new glass centrifuge tube. The remaining organic fraction was rinsed three more times with additions of 1 to 2 mL of cold 50:50 methanol:water. All aqueous rinses were combined for each sample and ~2mL of cold dichloromethane was added to the combined aqueous layer. Tubes were shaken and centrifuged at 4,300 rpm for 2 minutes at 4°C. The aqueous layer was removed to a new glass vial and dried under N2 gas. The remaining organic layer in the bead beating tubes was transferred into the glass centrifuge tube and the bead beating tube was rinsed two more times with cold organic solvent. The combined organic rinses were centrifuged, transferred to a new glass vial, and dried under N2 gas. Dried aqueous fractions were re-dissolved in 380 µL of water. Dried organic fractions were re-dissolved in 380 µL of 1:1 water:acetonitrile. 20 µL of isotope-labeled injection standards in water were added to both fractions. Media blanks were extracted alongside samples as methodological blanks.","PROCESSING_STORAGE_CONDITIONS":"On ice","EXTRACTION_METHOD":"Bligh-Dyer","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"See attached summary.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity I-Class","COLUMN_NAME":"Waters Acquity UPLC HSS Cyano (100 x 2.1mm, 1.8um)","METHODS_FILENAME":"Ingalls_Lab_LC_Methods.txt"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"Ingalls_Lab_MS_Methods.txt"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF hybrid Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"See attached protocol.","MS_RESULTS_FILE":"ST001514_AN002508_Results.txt UNITS:Adjusted and normalized peak areas  Has m/z:Yes Has RT:Yes RT units:Seconds"}

}