{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001515","ANALYSIS_ID":"AN002513","VERSION":"1","CREATED_ON":"November 3, 2020, 4:46 pm"},

"PROJECT":{"PROJECT_TITLE":"Effects of glucagon infusion on plasma metabolome in individuals with overweight/obesity","PROJECT_SUMMARY":"Objective: We sought to identify the circulating metabolites that would serve as markers of glucagon action. Design: In this study, we performed a continuous 72-hour glucagon infusion in healthy individuals with overweight/obesity. Participants were randomized to either glucagon (12.5 ng/kg/min) (GCG 12.5) or glucagon (25 ng/kg/min) GCG 25 or a placebo control were included. A comprehensive metabolomics analysis was then performed from plasma isolated at several time points during the infusion to identify markers of glucagon activity.","INSTITUTE":"Translational Research Institute- AdventHealth Orlando","LAST_NAME":"Whytock","FIRST_NAME":"Katie","ADDRESS":"301 E Princeton St, FL, USA, 32804","EMAIL":"Katie.Whytock@adventhealth.com","PHONE":"3219459982","FUNDING_SOURCE":"Sanofi"},

"STUDY":{"STUDY_TITLE":"A Metabolomic Signature of Glucagon Action in Healthy Individuals with Overweight/Obesity Humans","STUDY_SUMMARY":"Objective: We sought to identify the circulating metabolites that would serve as markers of glucagon action. Design: In this study, we performed a continuous 72-hour glucagon infusion in healthy individuals with overweight/obesity. Participants were randomized to either glucagon (12.5 ng/kg/min) (GCG 12.5) or glucagon (25 ng/kg/min) GCG 25 or a placebo control were included. A comprehensive metabolomics analysis was then performed from plasma isolated at several time points during the infusion to identify markers of glucagon activity.","INSTITUTE":"Translational Research Institute- AdventHealth Orlando","LAST_NAME":"Whytock","FIRST_NAME":"Katie","ADDRESS":"301 E Princeton St, Orlando, FL, 32804","EMAIL":"Katie.Whytock@adventhealth.com","PHONE":"321-945-9982","NUM_GROUPS":"3","TOTAL_SUBJECTS":"33"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00006-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00007-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00008-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00009-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00010-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00011-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00012-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00013-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00014-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00015-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00016-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00017-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00018-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00019-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
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{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00020-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00021-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00022-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00023-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00024-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00025-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00026-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00027-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00028-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
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{
"Subject ID":"LD1",
"Sample ID":"FLHP-00029-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00030-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00031-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00032-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00033-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00034-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
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{
"Subject ID":"HD4",
"Sample ID":"FLHP-00035-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00036-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00037-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00038-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00039-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00040-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00041-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00042-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00043-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00044-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00045-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00046-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00047-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00048-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00049-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00050-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00051-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00052-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
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{
"Subject ID":"HD5",
"Sample ID":"FLHP-00053-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00054-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00055-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00056-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00057-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00058-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00059-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00060-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00061-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00062-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00063-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00064-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00065-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00066-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00067-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00068-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00069-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00070-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00071-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00072-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
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{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00073-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
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{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00074-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00075-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00076-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00077-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00078-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
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{
"Subject ID":"LD3",
"Sample ID":"FLHP-00079-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00080-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
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"Sample ID":"FLHP-00081-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
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{
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{
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{
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},
{
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},
{
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},
{
"Subject ID":"HD7",
"Sample ID":"FLHP-00090-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
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"Subject ID":"HD7",
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"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
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},
{
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{
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},
{
"Subject ID":"Placebo6",
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},
{
"Subject ID":"Placebo6",
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{
"Subject ID":"Placebo6",
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{
"Subject ID":"LD4",
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},
{
"Subject ID":"LD4",
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},
{
"Subject ID":"LD4",
"Sample ID":"FLHP-00100-01",
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},
{
"Subject ID":"LD4",
"Sample ID":"FLHP-00101-01",
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},
{
"Subject ID":"LD4",
"Sample ID":"FLHP-00102-01",
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},
{
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{
"Subject ID":"Placebo7",
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},
{
"Subject ID":"Placebo7",
"Sample ID":"FLHP-00105-01",
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},
{
"Subject ID":"Placebo7",
"Sample ID":"FLHP-00106-01",
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},
{
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"Sample ID":"FLHP-00107-01",
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},
{
"Subject ID":"Placebo7",
"Sample ID":"FLHP-00108-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00109-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00110-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00111-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00112-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00113-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00114-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00115-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00116-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00117-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00118-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00119-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00120-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD6",
"Sample ID":"FLHP-00121-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD6",
"Sample ID":"FLHP-00122-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD6",
"Sample ID":"FLHP-00123-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD6",
"Sample ID":"FLHP-00124-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00125-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00126-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00127-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00128-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00129-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00130-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00131-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00132-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00133-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00134-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00135-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00136-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00137-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00138-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00139-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00140-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00141-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00142-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00143-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00144-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00145-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00146-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00147-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00148-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00149-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00150-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00151-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00152-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00153-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00154-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00155-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00156-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00157-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00158-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00159-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00160-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00161-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00162-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00163-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00164-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00165-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00166-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00167-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00168-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00169-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00170-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00171-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00172-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00173-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00174-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00175-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00176-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00177-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00178-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00179-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00180-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00181-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00182-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00183-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00184-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00185-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00186-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00187-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00188-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00189-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00190-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00191-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00192-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Samples were homogenized and subjected to methanol extraction then split into aliquots for analysis","SAMPLE_TYPE":"Blood (plasma)"},

"TREATMENT":{"TREATMENT_SUMMARY":"Study subjects were admitted to the Clinical Research Unit (CRU) at the Translational Research Institute (TRI) at AdventHealth for 5 overnight stays. The first 2 overnight stays were used as baseline. On the 2nd day (Day -1), a baseline blood draw (-24 hours) was performed. On Day 1, a blood draw was again taken approximately 30 minutes prior to the start of the glucagon infusion. The study subjects began a ramped infusion (first 4 hours) of either saline (Placebo), Low Dose Glucagon (12.5ng/kg/min) or High Dose Glucagon (25ng/kg/min) for the next 72 hours (Inpatient). The Glucagon (GlucaGen, Novo Nordisk, Bagsværd Denmark) was administered under an FDA-approved IND (#136634). Intravenous (IV) bags of the study medication were hung together on an IV pole and changed out every 24 hours to provide a continuous infusion. With the exception of the 4-hour time point, all draws were performed in the overnight fasted state."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Briefly, samples were homogenized and subjected to methanol extraction then split into aliquots for analysis by ultrahigh performance liquid chromatography/mass spectrometry (UHPLC/MS) in the positive (two methods) and negative (two methods) mode. Metabolites were then identified by automated comparison of ion features to a reference library of chemical standards followed by visual inspection for quality control."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity BEH C18 (100 x 2mm, 1.7um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Proprietary analytical software for integration and peak picking"},

"MS_METABOLITE_DATA":{
"Units":"scaled units",

"Data":[{"Metabolite":"(14 or 15)-methylpalmitate (a17:0 or i17:0)","FLHP-00006-01":"1.1107","FLHP-00007-01":"0.7256","FLHP-00008-01":"0.2785","FLHP-00009-01":"0.6369","FLHP-00010-01":"0.7928","FLHP-00011-01":"0.4074","FLHP-00012-01":"1.1838","FLHP-00013-01":"1.2262","FLHP-00014-01":"0.3957","FLHP-00015-01":"0.9571","FLHP-00016-01":"0.6378","FLHP-00017-01":"1.8444","FLHP-00018-01":"1.8211","FLHP-00019-01":"1.0000","FLHP-00020-01":"0.9201","FLHP-00021-01":"1.0157","FLHP-00022-01":"1.2762","FLHP-00023-01":"1.0188","FLHP-00024-01":"0.4636","FLHP-00025-01":"2.3808","FLHP-00026-01":"0.7196","FLHP-00027-01":"2.9098","FLHP-00028-01":"1.4692","FLHP-00029-01":"1.0576","FLHP-00030-01":"0.7934","FLHP-00031-01":"0.9130","FLHP-00032-01":"0.7500","FLHP-00033-01":"3.0086","FLHP-00034-01":"1.3994","FLHP-00035-01":"0.7184","FLHP-00036-01":"0.8313","FLHP-00037-01":"0.9082","FLHP-00038-01":"1.5286","FLHP-00039-01":"1.1397","FLHP-00040-01":"0.7535","FLHP-00041-01":"4.4191","FLHP-00042-01":"2.7414","FLHP-00043-01":"1.5446","FLHP-00044-01":"1.6309","FLHP-00045-01":"1.8740","FLHP-00046-01":"1.1207","FLHP-00047-01":"0.9750","FLHP-00048-01":"0.8065","FLHP-00049-01":"0.5992","FLHP-00050-01":"0.6585","FLHP-00051-01":"0.5902","FLHP-00052-01":"0.9890","FLHP-00053-01":"0.7322","FLHP-00054-01":"0.5186","FLHP-00055-01":"1.7336","FLHP-00056-01":"2.8717","FLHP-00057-01":"2.5540","FLHP-00058-01":"1.4267","FLHP-00059-01":"0.8456","FLHP-00060-01":"0.8905","FLHP-00061-01":"0.9700","FLHP-00062-01":"0.8630","FLHP-00063-01":"0.3969","FLHP-00064-01":"0.3405","FLHP-00065-01":"0.6417","FLHP-00066-01":"0.9373","FLHP-00067-01":"1.0433","FLHP-00068-01":"0.7327","FLHP-00069-01":"0.8527","FLHP-00070-01":"0.8696","FLHP-00071-01":"0.7805","FLHP-00072-01":"1.1139","FLHP-00073-01":"1.8830","FLHP-00074-01":"1.1187","FLHP-00075-01":"0.8920","FLHP-00076-01":"1.2380","FLHP-00077-01":"0.4868","FLHP-00078-01":"2.6665","FLHP-00079-01":"1.1353","FLHP-00080-01":"1.0562","FLHP-00081-01":"0.6579","FLHP-00082-01":"0.6733","FLHP-00083-01":"1.2422","FLHP-00084-01":"0.8879","FLHP-00085-01":"0.7010","FLHP-00086-01":"0.9643","FLHP-00087-01":"0.2654","FLHP-00088-01":"0.6035","FLHP-00089-01":"1.1446","FLHP-00090-01":"1.4934","FLHP-00091-01":"0.8190","FLHP-00092-01":"0.6584","FLHP-00093-01":"0.6512","FLHP-00094-01":"0.7422","FLHP-00095-01":"1.2045","FLHP-00096-01":"0.9024","FLHP-00097-01":"0.9812","FLHP-00098-01":"0.6732","FLHP-00099-01":"0.6553","FLHP-00100-01":"0.7000","FLHP-00101-01":"0.8372","FLHP-00102-01":"0.5501","FLHP-00103-01":"2.5661","FLHP-00104-01":"1.0432","FLHP-00105-01":"0.7364","FLHP-00106-01":"1.3009","FLHP-00107-01":"0.7752","FLHP-00108-01":"1.7934","FLHP-00109-01":"0.7375","FLHP-00110-01":"0.6167","FLHP-00111-01":"0.6573","FLHP-00112-01":"1.7071","FLHP-00113-01":"0.3083","FLHP-00114-01":"0.6421","FLHP-00115-01":"1.9127","FLHP-00116-01":"0.8009","FLHP-00117-01":"0.6555","FLHP-00118-01":"2.1617","FLHP-00119-01":"0.9853","FLHP-00120-01":"2.1201","FLHP-00121-01":"2.6355","FLHP-00122-01":"1.3564","FLHP-00123-01":"0.8547","FLHP-00124-01":"0.9383","FLHP-00125-01":"1.2180","FLHP-00126-01":"0.9318","FLHP-00127-01":"1.6352","FLHP-00128-01":"2.3856","FLHP-00129-01":"1.3597","FLHP-00130-01":"1.0896","FLHP-00131-01":"1.1328","FLHP-00132-01":"0.5569","FLHP-00133-01":"1.3141","FLHP-00134-01":"1.3236","FLHP-00135-01":"0.8837","FLHP-00136-01":"1.2219","FLHP-00137-01":"1.0278","FLHP-00138-01":"0.5962","FLHP-00139-01":"1.0332","FLHP-00140-01":"1.2964","FLHP-00141-01":"0.8767","FLHP-00142-01":"0.3843","FLHP-00143-01":"0.9212","FLHP-00144-01":"1.7928","FLHP-00145-01":"1.1274","FLHP-00146-01":"1.1671","FLHP-00147-01":"1.9922","FLHP-00148-01":"2.8166","FLHP-00149-01":"1.1151","FLHP-00150-01":"0.5357","FLHP-00151-01":"1.3049","FLHP-00152-01":"1.0357","FLHP-00153-01":"0.6270","FLHP-00154-01":"1.0806","FLHP-00155-01":"0.5914","FLHP-00156-01":"2.8894","FLHP-00157-01":"0.8066","FLHP-00158-01":"1.0004","FLHP-00159-01":"1.1827","FLHP-00160-01":"1.3222","FLHP-00161-01":"1.2375","FLHP-00162-01":"0.9296","FLHP-00163-01":"1.4575","FLHP-00164-01":"2.7127","FLHP-00165-01":"0.7335","FLHP-00166-01":"1.1712","FLHP-00167-01":"0.4851","FLHP-00168-01":"1.4414","FLHP-00169-01":"0.8028","FLHP-00170-01":"1.9516","FLHP-00171-01":"1.1199","FLHP-00172-01":"1.1591","FLHP-00173-01":"1.2197","FLHP-00174-01":"2.5567","FLHP-00175-01":"0.8213","FLHP-00176-01":"0.9695","FLHP-00177-01":"0.4616","FLHP-00178-01":"0.9889","FLHP-00179-01":"2.1772","FLHP-00180-01":"1.4247","FLHP-00181-01":"4.4070","FLHP-00182-01":"1.0581","FLHP-00183-01":"0.3667","FLHP-00184-01":"1.4188","FLHP-00185-01":"1.4138","FLHP-00186-01":"1.2055","FLHP-00187-01":"0.8756","FLHP-00188-01":"1.5036","FLHP-00189-01":"0.8299","FLHP-00190-01":"1.4159","FLHP-00191-01":"1.4391","FLHP-00192-01":"0.6105"},{"Metabolite":"(16 or 17)-methylstearate (a19:0 or 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Index":"2440","MASS":"215.1289","PUBCHEM":"15816","KEGG":""},{"Metabolite":"urate","Retention Index":"757.1","MASS":"167.0211","PUBCHEM":"1175","KEGG":"C00366"},{"Metabolite":"uridine","Retention Index":"1457.6","MASS":"243.0623","PUBCHEM":"6029","KEGG":"C00299"},{"Metabolite":"ursodeoxycholate","Retention Index":"5055","MASS":"391.2854","PUBCHEM":"31401","KEGG":"C07880"},{"Metabolite":"vanillylmandelate (VMA)","Retention Index":"941","MASS":"197.0456","PUBCHEM":"1245","KEGG":"C05584"}]
}

}