{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001515","ANALYSIS_ID":"AN002514","VERSION":"1","CREATED_ON":"November 3, 2020, 4:46 pm"},

"PROJECT":{"PROJECT_TITLE":"Effects of glucagon infusion on plasma metabolome in individuals with overweight/obesity","PROJECT_SUMMARY":"Objective: We sought to identify the circulating metabolites that would serve as markers of glucagon action. Design: In this study, we performed a continuous 72-hour glucagon infusion in healthy individuals with overweight/obesity. Participants were randomized to either glucagon (12.5 ng/kg/min) (GCG 12.5) or glucagon (25 ng/kg/min) GCG 25 or a placebo control were included. A comprehensive metabolomics analysis was then performed from plasma isolated at several time points during the infusion to identify markers of glucagon activity.","INSTITUTE":"Translational Research Institute- AdventHealth Orlando","LAST_NAME":"Whytock","FIRST_NAME":"Katie","ADDRESS":"301 E Princeton St, FL, USA, 32804","EMAIL":"Katie.Whytock@adventhealth.com","PHONE":"3219459982","FUNDING_SOURCE":"Sanofi"},

"STUDY":{"STUDY_TITLE":"A Metabolomic Signature of Glucagon Action in Healthy Individuals with Overweight/Obesity Humans","STUDY_SUMMARY":"Objective: We sought to identify the circulating metabolites that would serve as markers of glucagon action. Design: In this study, we performed a continuous 72-hour glucagon infusion in healthy individuals with overweight/obesity. Participants were randomized to either glucagon (12.5 ng/kg/min) (GCG 12.5) or glucagon (25 ng/kg/min) GCG 25 or a placebo control were included. A comprehensive metabolomics analysis was then performed from plasma isolated at several time points during the infusion to identify markers of glucagon activity.","INSTITUTE":"Translational Research Institute- AdventHealth Orlando","LAST_NAME":"Whytock","FIRST_NAME":"Katie","ADDRESS":"301 E Princeton St, Orlando, FL, 32804","EMAIL":"Katie.Whytock@adventhealth.com","PHONE":"321-945-9982","NUM_GROUPS":"3","TOTAL_SUBJECTS":"33"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00006-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00007-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00008-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00009-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00010-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD1",
"Sample ID":"FLHP-00011-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00012-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00013-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00014-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00015-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD2",
"Sample ID":"FLHP-00016-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00017-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00018-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00019-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
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{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00020-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00021-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo1",
"Sample ID":"FLHP-00022-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00023-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00024-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00025-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00026-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00027-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD3",
"Sample ID":"FLHP-00028-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
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{
"Subject ID":"LD1",
"Sample ID":"FLHP-00029-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00030-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00031-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00032-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00033-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD1",
"Sample ID":"FLHP-00034-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00035-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00036-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00037-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00038-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00039-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD4",
"Sample ID":"FLHP-00040-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00041-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00042-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00043-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00044-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00045-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo2",
"Sample ID":"FLHP-00046-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00047-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00048-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00049-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00050-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00051-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo3",
"Sample ID":"FLHP-00052-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00053-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00054-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00055-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00056-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00057-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD5",
"Sample ID":"FLHP-00058-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00059-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00060-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00061-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00062-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD6",
"Sample ID":"FLHP-00063-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00064-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00065-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00066-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00067-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00068-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD2",
"Sample ID":"FLHP-00069-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00070-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00071-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00072-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
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{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00073-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
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{
"Subject ID":"Placebo4",
"Sample ID":"FLHP-00074-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00075-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00076-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00077-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00078-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
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{
"Subject ID":"LD3",
"Sample ID":"FLHP-00079-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD3",
"Sample ID":"FLHP-00080-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo5",
"Sample ID":"FLHP-00081-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
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{
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{
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{
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},
{
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{
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},
{
"Subject ID":"HD7",
"Sample ID":"FLHP-00090-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
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"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
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},
{
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{
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},
{
"Subject ID":"Placebo6",
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},
{
"Subject ID":"Placebo6",
"Sample ID":"FLHP-00096-01",
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{
"Subject ID":"Placebo6",
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{
"Subject ID":"LD4",
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{
"Subject ID":"LD4",
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},
{
"Subject ID":"LD4",
"Sample ID":"FLHP-00100-01",
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},
{
"Subject ID":"LD4",
"Sample ID":"FLHP-00101-01",
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},
{
"Subject ID":"LD4",
"Sample ID":"FLHP-00102-01",
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},
{
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"Subject ID":"Placebo7",
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},
{
"Subject ID":"Placebo7",
"Sample ID":"FLHP-00105-01",
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},
{
"Subject ID":"Placebo7",
"Sample ID":"FLHP-00106-01",
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},
{
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"Sample ID":"FLHP-00107-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo7",
"Sample ID":"FLHP-00108-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00109-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00110-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00111-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00112-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00113-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD8",
"Sample ID":"FLHP-00114-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00115-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00116-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00117-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00118-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00119-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD5",
"Sample ID":"FLHP-00120-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD6",
"Sample ID":"FLHP-00121-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD6",
"Sample ID":"FLHP-00122-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD6",
"Sample ID":"FLHP-00123-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD6",
"Sample ID":"FLHP-00124-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00125-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00126-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00127-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00128-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo8",
"Sample ID":"FLHP-00129-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00130-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00131-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00132-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00133-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD7",
"Sample ID":"FLHP-00134-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00135-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00136-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00137-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00138-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00139-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD8",
"Sample ID":"FLHP-00140-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00141-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00142-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00143-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00144-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00145-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD9",
"Sample ID":"FLHP-00146-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00147-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00148-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00149-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00150-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00151-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD9",
"Sample ID":"FLHP-00152-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00153-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00154-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00155-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00156-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00157-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD10",
"Sample ID":"FLHP-00158-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00159-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00160-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00161-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00162-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00163-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo9",
"Sample ID":"FLHP-00164-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00165-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00166-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00167-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00168-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00169-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"Placebo10",
"Sample ID":"FLHP-00170-01",
"Factors":{"Group":"Placebo","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00171-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00172-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00173-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00174-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD10",
"Sample ID":"FLHP-00175-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00176-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00177-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00178-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00179-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00180-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD11",
"Sample ID":"FLHP-00181-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00182-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00183-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00184-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00185-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"HD11",
"Sample ID":"FLHP-00186-01",
"Factors":{"Group":"High-Dose","TIMEPOINT":"+23HR POST INFUSION"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00187-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"BASELINE"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00188-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+71HR POST INFUSION"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00189-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+4HR POST INFUSION"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00190-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"-0.5HR"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00191-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+47HR POST INFUSION"}
},
{
"Subject ID":"LD12",
"Sample ID":"FLHP-00192-01",
"Factors":{"Group":"Low-Dose","TIMEPOINT":"+23HR POST INFUSION"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Samples were homogenized and subjected to methanol extraction then split into aliquots for analysis","SAMPLE_TYPE":"Blood (plasma)"},

"TREATMENT":{"TREATMENT_SUMMARY":"Study subjects were admitted to the Clinical Research Unit (CRU) at the Translational Research Institute (TRI) at AdventHealth for 5 overnight stays. The first 2 overnight stays were used as baseline. On the 2nd day (Day -1), a baseline blood draw (-24 hours) was performed. On Day 1, a blood draw was again taken approximately 30 minutes prior to the start of the glucagon infusion. The study subjects began a ramped infusion (first 4 hours) of either saline (Placebo), Low Dose Glucagon (12.5ng/kg/min) or High Dose Glucagon (25ng/kg/min) for the next 72 hours (Inpatient). The Glucagon (GlucaGen, Novo Nordisk, Bagsværd Denmark) was administered under an FDA-approved IND (#136634). Intravenous (IV) bags of the study medication were hung together on an IV pole and changed out every 24 hours to provide a continuous infusion. With the exception of the 4-hour time point, all draws were performed in the overnight fasted state."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Briefly, samples were homogenized and subjected to methanol extraction then split into aliquots for analysis by ultrahigh performance liquid chromatography/mass spectrometry (UHPLC/MS) in the positive (two methods) and negative (two methods) mode. Metabolites were then identified by automated comparison of ion features to a reference library of chemical standards followed by visual inspection for quality control."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Proprietary analytical software for integration and peak picking"},

"MS_METABOLITE_DATA":{
"Units":"scaled units",

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}

}