{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001609","ANALYSIS_ID":"AN002643","VERSION":"1","CREATED_ON":"November 30, 2020, 11:40 am"},

"PROJECT":{"PROJECT_TITLE":"Comparative metabolomics analysis of two Saccharomyces cerevisiae strains: the wild type and mtl1Δ, which carries a deletion of the mechanosensor Mtl1p","PROJECT_SUMMARY":"To gather more in-depth knowledge of the Mtl1p mechanosensor's role in Saccharomyces cerevisiae metabolism, we conducted a comparative metabolomic analysis of two Saccharomyces cerevisiae strains: the wild type and mtl1Δ, which carries a deletion of the mechanosensor Mtl1p. Both strains were grown under normal conditions at 27°C. The most significant metabolic changes between these strains were related to amino acid metabolism, purine metabolism, and carboxylic acid metabolism.","INSTITUTE":"University of Puerto Rico, Medical Sciences Campus","DEPARTMENT":"Biochemistry","LAST_NAME":"Chorna","FIRST_NAME":"Nataliya","ADDRESS":"University of Puerto Rico, Medical Sciences Campus, Department of Biochemistry, Main Building, 6th Floor, Room A-632, San Juan, PR 00935","EMAIL":"nataliya.chorna@upr.edu","PHONE":"787-758-2525 x 1640","FUNDING_SOURCE":"NIGMS-NIH-PRINBRE-P20GM103475","CONTRIBUTORS":"Nelson Martínez–Matías, Sahily González–Crespo"},

"STUDY":{"STUDY_TITLE":"Comparative metabolomics analysis of two Saccharomyces cerevisiae strains: the wild type and mtl1Δ, which carries a deletion of the mechanosensor Mtl1p.","STUDY_SUMMARY":"To gather more in-depth knowledge of the Mtl1p mechanosensor's role in Saccharomyces cerevisiae metabolism, we conducted a comparative metabolomic analysis of two Saccharomyces cerevisiae strains: the wild type and mtl1Δ, which carries a deletion of the mechanosensor Mtl1p. Both strains were grown under normal conditions at 27°C. The most significant metabolic changes between these strains were related to amino acid metabolism, purine metabolism, and carboxylic acid metabolism.","INSTITUTE":"University of Puerto Rico, Medical Sciences Campus","LAST_NAME":"Chorna","FIRST_NAME":"Nataliya","ADDRESS":"University of Puerto Rico, Medical Sciences Campus, Department of Biochemistry, Main Building, 6th Floor, Room A-632, San Juan, PR 00935","EMAIL":"nataliya.chorna@upr.edu","PHONE":"787-758-2525 ext 1640","NUM_GROUPS":"2","TOTAL_SUBJECTS":"26"},

"SUBJECT":{"SUBJECT_TYPE":"Yeast","SUBJECT_SPECIES":"Saccharomyces cerevisiae","TAXONOMY_ID":"4932","GENOTYPE_STRAIN":"BY4742, YGR023w","CELL_BIOSOURCE_OR_SUPPLIER":"Open Biosystems"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"BY4742-1",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-1.qgd","OD600":"0.625"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-2",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-2.qgd","OD600":"0.627"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-3",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-3.qgd","OD600":"0.594"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-4",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-4.qgd","OD600":"0.661"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-5",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-5.qgd","OD600":"0.689"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-6",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-6.qgd","OD600":"0.644"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-7",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-7.qgd","OD600":"0.667"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-8",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-8.qgd","OD600":"0.678"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-9",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-9.qgd","OD600":"0.660"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-10",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-10.qgd","OD600":"0.663"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-11",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-11.qgd","OD600":"0.682"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-12",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-12.qgd","OD600":"0.613"}
},
{
"Subject ID":"-",
"Sample ID":"BY4742-13",
"Factors":{"Strain":"Wild-type"},
"Additional sample data":{"RAW_FILE_NAME":"BY4742-13.qgd","OD600":"0.683"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-1",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-1.qgd","OD600":"0.652"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-2",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-2.qgd","OD600":"0.627"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-3",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-3.qgd","OD600":"0.602"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-4",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-4.qgd","OD600":"0.661"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-5",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-5.qgd","OD600":"0.639"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-6",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-6.qgd","OD600":"0.713"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-7",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-7.qgd","OD600":"0.635"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-8",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-8.qgd","OD600":"0.694"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-9",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-9.qgd","OD600":"0.685"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-10",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-10.qgd","OD600":"0.628"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-11",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-11.qgd","OD600":"0.681"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-12",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-12.qgd","OD600":"0.684"}
},
{
"Subject ID":"-",
"Sample ID":"YGR023w-13",
"Factors":{"Strain":"mtl1Δ"},
"Additional sample data":{"RAW_FILE_NAME":"YGR023w-13.qgd","OD600":"0.683"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Cells were grown at 27°C, 210 rpm in 5 mL of complete synthetic medium (CSM, 0.67% Nitrogen base without amino acids and ammonium sulfate, 2% glucose). The next day, cells were replaced with fresh CSM and grow at 27°C to reach an OD of 0.6 - 0.7. 25 ml of each sample were harvested by centrifugation at 3,838 x g for 5 min at 4°C, washed with 1 ml ice cold sterile water, quenched using ice cold methanol, and stored at -80°C.","SAMPLE_TYPE":"Yeast cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"No treatment"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Extraction of metabolites was performed by homogenization in 1 mL of cold methanol/H2O (1:1) extraction solution and centrifugated at 167 x g at 4°C for 5 min. Supernatants were collected and evaporated to dryness in a nitrogen stream stream at 50 ºC (RapidVap, Labconco). Nitrilation was performed by adding 150 μL of 0.2 mM hydroxylammonium chloride in pyridine to the dried sample, and then heating at 90ºC for 40 minutes. After that, acetylation was performed by adding 250 μL of acetic anhydride, and then heating at 90 ºC for 60 minutes. After that, the sample was dried again in a nitrogen gas stream, and then redissolved in 400 μL of ethyl acetate. Derivatized samples were collected and stored at -20ºC."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Shimadzu GCMS-QP2010 ultra","COLUMN_NAME":"Shimadzu SH-RXI (30M x 0.25mm x 0.25um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Shimadzu QP2010 Ultra","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"Inlet temperature was 280°C; ion source temperature was 200 °C. MS conditions were set as follows: SIM acquisition mode, electron energy of 70 eV, quadrupole scan range of m/z 115; 187; 212;314. Data were processed using the GCMS Solution Postrun Analysis software (Shimadzu Inc) for metabolites identification from their electron impact mass spectra by comparison to the NIST 2014 spectral mass library."},

"MS_METABOLITE_DATA":{
"Units":"mM",

"Data":[{"Metabolite":"Glucose","BY4742-1":"0.038140","BY4742-2":"0.039380","BY4742-3":"0.060200","BY4742-4":"0.050120","BY4742-5":"0.069050","BY4742-6":"0.041340","BY4742-7":"0.042350","BY4742-8":"0.037060","BY4742-9":"0.085690","BY4742-10":"0.038010","BY4742-11":"0.051820","BY4742-12":"0.050020","BY4742-13":"0.044300","YGR023w-1":"0.12715","YGR023w-2":"0.06061","YGR023w-3":"0.04862","YGR023w-4":"0.06086","YGR023w-5":"0.03731","YGR023w-6":"0.03711","YGR023w-7":"0.03789","YGR023w-8":"0.03592","YGR023w-9":"0.04124","YGR023w-10":"0.0383","YGR023w-11":"0.0839","YGR023w-12":"0.04703","YGR023w-13":"0.05165"}],

"Metabolites":[{"Metabolite":"Glucose","quantified m/z":"180","KEGG":"C00031"}]
}

}