{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001623","ANALYSIS_ID":"AN002658","VERSION":"1","CREATED_ON":"December 11, 2020, 10:21 am"}, "PROJECT":{"PROJECT_TITLE":"Raffinose underlies diet-dependent epithelial response to stress","PROJECT_TYPE":"Targeted metabolomics analysis with raffinose-rich diet in mouse ileum","PROJECT_SUMMARY":"The raffinose-rich diet was supplemented with raffinose, a significant increase in ileum fructose in stressed mice could be observed.","INSTITUTE":"China Pharmaceutical University","DEPARTMENT":"School of Medicine","LABORATORY":"Metabonomics","LAST_NAME":"Hou","FIRST_NAME":"Yuanlong","ADDRESS":"tongjiaxiang, nanjing, jiangsu, 210000, China","EMAIL":"jian2103@163.com","PHONE":"18851105337","CONTRIBUTORS":"Xiao Zheng Haiping Hao"}, "STUDY":{"STUDY_TITLE":"Targeted metabolomics analysis with raffinose-rich diet in mouse ileum","STUDY_TYPE":"Analysis of fructose level in ileum of stressed mouse fed with raffinose-rich diet","STUDY_SUMMARY":"Purified diet (AIN-93G) supplemented with raffinose was prepared. The mice were maintained on the separate diet for at least 1 week before the initiation of experiment.Chronic restraint stress (RS) in mice was performed 14 days.After sacrifice, the ileum of mice were used to analysis.","INSTITUTE":"China Pharmaceutical University","DEPARTMENT":"School of Medicine","LABORATORY":"Metabonomics","LAST_NAME":"Hou","FIRST_NAME":"Yuanlong","ADDRESS":"tongjiaxiang, nanjing, jiangsu, 210000, China","EMAIL":"jian2103@163.com","PHONE":"18851105337"}, "SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Female"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"S1", "Factors":{"Treatment":"Stress"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INESTINAL-S1"} }, { "Subject ID":"-", "Sample ID":"S2", "Factors":{"Treatment":"Stress"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INESTINAL-S2"} }, { "Subject ID":"-", "Sample ID":"S3", "Factors":{"Treatment":"Stress"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INESTINAL-S3"} }, { "Subject ID":"-", "Sample ID":"S4", "Factors":{"Treatment":"Stress"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INESTINAL-S4"} }, { "Subject ID":"-", "Sample ID":"C1", "Factors":{"Treatment":"Con"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INESTINAL-C1"} }, { "Subject ID":"-", "Sample ID":"C2", "Factors":{"Treatment":"Con"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INESTINAL-C2"} }, { "Subject ID":"-", "Sample ID":"C3", "Factors":{"Treatment":"Con"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INESTINAL-C3"} }, { "Subject ID":"-", "Sample ID":"C4", "Factors":{"Treatment":"Con"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INESTINAL-C4"} }, { "Subject ID":"-", "Sample ID":"R1", "Factors":{"Treatment":"Raffinose"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INTESTINAL-R1"} }, { "Subject ID":"-", "Sample ID":"R3", "Factors":{"Treatment":"Raffinose"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INTESTINAL-R3"} }, { "Subject ID":"-", "Sample ID":"R4", "Factors":{"Treatment":"Raffinose"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INTESTINAL-R4"} }, { "Subject ID":"-", "Sample ID":"R5", "Factors":{"Treatment":"Raffinose"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INTESTINAL-R5"} }, { "Subject ID":"-", "Sample ID":"R6", "Factors":{"Treatment":"Raffinose"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INTESTINAL-R6"} }, { "Subject ID":"-", "Sample ID":"R7", "Factors":{"Treatment":"Raffinose"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INTESTINAL-R7"} }, { "Subject ID":"-", "Sample ID":"R8", "Factors":{"Treatment":"Raffinose"}, "Additional sample data":{"RAW_FILE_NAME":"20191205HYL-INTESTINAL-R8"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"jejunum tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum. It was reconstituted with 100 μL of methanol: water solution (50:50, v/v) before analysis.","SAMPLE_TYPE":"Intestine"}, "TREATMENT":{"TREATMENT_SUMMARY":"PD was supplemented with raffinose, a significant increase in intestinal fructose in stressed mice could be observed."}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Briefly, tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum."}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Waters Acquity H-Class","COLUMN_NAME":"Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_NAME":"Xevo G2-XS, Waters","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"MS MassLynx v4.1 Quanlynx v4.1"}, "MS_METABOLITE_DATA":{ "Units":"relative peak area", "Data":[{"Metabolite":"Fructose","C1":"0.0347609","C2":"0.040259","C3":"0.04000952","C4":"0.0364934","S1":"0.025058","S2":"0.02226","S3":"0.013987","S4":"0.01046851","R1":"0.07282454","R3":"0.04740064","R4":"0.05582034","R5":"0.0831141","R6":"0.07902627","R7":"0.07224646","R8":"0.05783501"},{"Metabolite":"Glucose","C1":"0.0119652","C2":"0.0275589","C3":"0.01848641","C4":"0.0119649","S1":"0.04606187","S2":"0.03815228","S3":"0.0118872","S4":"0.0306539","R1":"0.02514915","R3":"0.01567248","R4":"0.04050696","R5":"0.01958676","R6":"0.03802045","R7":"0.0201069","R8":"0.02894439"}], "Metabolites":[{"metabolite_name":"Fructose"},{"metabolite_name":"Glucose"}] } }