{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001755","ANALYSIS_ID":"AN002859","VERSION":"1","CREATED_ON":"April 29, 2021, 5:15 pm"},

"PROJECT":{"PROJECT_SUMMARY":"Metabolomics analysis of cancer-derived extracellular vesicles.","INSTITUTE":"National Center for Research in Energy and Materials - CNPEM","DEPARTMENT":"Brazilian Biosciences National Laboratory - LNBio","LABORATORY":"Mass Spectrometry Laboratory","FIRST_NAME":"Ariane","ADDRESS":"R. Giuseppe Máximo Scolfaro, 10000, Bosque das Palmeiras, Campinas, SP, 13083-100, Brazil","EMAIL":"ariane.lopes@lnbio.cnpem.br","PHONE":"+55 19 3512-1276","PROJECT_TITLE":"A reductionist approach using primary and metastatic cell-derived extracellular vesicles reveals hub proteins associated with oral cancer prognosis","LAST_NAME":"Busso Lopes"},

"STUDY":{"STUDY_TITLE":"A reductionist approach using primary and metastatic cell-derived extracellular vesicles reveals hub proteins associated with oral cancer prognosis","STUDY_SUMMARY":"Oral squamous cell carcinoma (OSCC) has high mortality rates that are largely associated with lymph node metastasis. However, the molecular mechanisms that drive OSCC metastasis are unknown. Extracellular vesicles (EVs) are membrane-bound particles that play a role in intercellular communication and impact cancer development and progression. Thus, profiling EVs would be of great significance to decipher the role of EV cargo in OSCC metastasis. For that purpose, we used a reductionist approach to map the proteomic, miRNA, metabolomic, and lipidomic profiles of extracellular vesicles (EVs) derived from human primary tumor (SCC-9) cells and matched lymph node metastases (LN1) cells. Distinct omics profiles were associated with the metastatic phenotype, including 670 proteins, 217 miRNAs, 26 metabolites, and 64 lipids differentially abundant between LN1- and SCC-9-derived EVs. A multi-omics integration identified 11 ‘hub proteins’ significantly decreased at the metastatic site compared to primary tumor-derived EVs. We confirmed the validity of these findings with analysis of data from multiple public databases, and found that low abundance of seven hub proteins in metastatic EVs is correlated with reduced survival and tumor aggressiveness in cancer patients. In summary, this multi-omics approach identified proteins transported by EVs that are associated with metastasis, and which may potentially serve as prognostic markers in OSCC.","INSTITUTE":"National Center for Research in Energy and Materials","DEPARTMENT":"Brazilian Biosciences National Laboratory - LNBio","LABORATORY":"Mass Spectrometry Laboratory","LAST_NAME":"Busso Lopes","FIRST_NAME":"Ariane","ADDRESS":"R. Giuseppe Máximo Scolfaro, 10000","EMAIL":"ariane.lopes@lnbio.cnpem.br","NUM_GROUPS":"2","TOTAL_SUBJECTS":"10","PHONE":"+55 19 3512-1276"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENOTYPE_STRAIN":"SCC-9 and LN1","CELL_STRAIN_DETAILS":"Primary tumor (SCC-9) and metastatic (LN1) oral cancer cell lines"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SCC-9-EV",
"Sample ID":"SCC-9_1-EV intensity",
"Factors":{"Factor":"Primary tumor EV"},
"Additional sample data":{"RAW_FILE_NAME":"EV_SCC9_09fev2017.cdf"}
},
{
"Subject ID":"SCC-9-EV",
"Sample ID":"SCC-9_2-EV intensity",
"Factors":{"Factor":"Primary tumor EV"},
"Additional sample data":{"RAW_FILE_NAME":"EV_SCC9_24abril2017.cdf"}
},
{
"Subject ID":"SCC-9-EV",
"Sample ID":"SCC-9_3-EV intensity",
"Factors":{"Factor":"Primary tumor EV"},
"Additional sample data":{"RAW_FILE_NAME":"EV_SCC9_25abril2017.cdf"}
},
{
"Subject ID":"SCC-9-EV",
"Sample ID":"SCC-9_4-EV intensity",
"Factors":{"Factor":"Primary tumor EV"},
"Additional sample data":{"RAW_FILE_NAME":"SCC9_B.cdf"}
},
{
"Subject ID":"SCC-9-EV",
"Sample ID":"SCC-9_5-EV intensity",
"Factors":{"Factor":"Primary tumor EV"},
"Additional sample data":{"RAW_FILE_NAME":"SCC9_C.cdf"}
},
{
"Subject ID":"LN1-EV",
"Sample ID":"LN1_1-EV intensity",
"Factors":{"Factor":"Metastatic EV"},
"Additional sample data":{"RAW_FILE_NAME":"EV_LN1_02maio2017.cdf"}
},
{
"Subject ID":"LN1-EV",
"Sample ID":"LN1_2-EV intensity",
"Factors":{"Factor":"Metastatic EV"},
"Additional sample data":{"RAW_FILE_NAME":"EV_LN1_03maio2017.cdf"}
},
{
"Subject ID":"LN1-EV",
"Sample ID":"LN1_3-EV intensity",
"Factors":{"Factor":"Metastatic EV"},
"Additional sample data":{"RAW_FILE_NAME":"EV_LN1_07fev2017.cdf"}
},
{
"Subject ID":"LN1-EV",
"Sample ID":"LN1_4-EV intensity",
"Factors":{"Factor":"Metastatic EV"},
"Additional sample data":{"RAW_FILE_NAME":"LN1_A.cdf"}
},
{
"Subject ID":"LN1-EV",
"Sample ID":"LN1_5-EV intensity",
"Factors":{"Factor":"Metastatic EV"},
"Additional sample data":{"RAW_FILE_NAME":"LN1_B.cdf"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"EVs were isolated from SCC-9 and LN1 cell cultures through differential centrifugation. Cells were cultured until 80% cell confluence in 150 mm diameter plates, washed three times with phosphate buffered saline (PBS) and further cultivated for 48 h in media without FBS, at 37°C and 5% CO2. After serum deprivation treatment, the conditioned media (200 mL) was collected and centrifuged at 200 x g for 5 min, 2,000 x g for 15 min, 3,500 x g for 30 min and 10,000 x g for 90 min. The cleared supernatant was further ultracentrifuged at 100,000 x g for 90 min at 4°C and vesicle-containing pellets were washed with PBS by ultracentrifugation for 1.5 h at the same speed. The samples were stored at -80°C until further use.","SAMPLE_TYPE":"Keratinocytes","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"EVs were not submitted to any treatment prior to metabolites extraction."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"SCC-9 and LN1-derived EVs (5x10e10 particles; 5 biological replicates for each group) were submitted to metabolite extraction using the MTBE method. Derivatization of metabolites was performed as outlined previously (Lisec et al., Nat Protoc. 2015;10(9):1457)."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Agilent DB-35MS (30m x 0,32mm x 0,25um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Leco Pegasus HT TOF","INSTRUMENT_TYPE":"GC-TOF","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"GC-TOF-MS data were obtained using a PAL-Combi XT autosampler (PAL System, Switzerland, http://www.palsystem.com/), coupled to an Agilent 7890 A gas chromatograph - Leco Pegasus HT time-of-flight mass spectrometer (LECO, USA) in both split (1:15 and 1:50) and splitless modes (Weckwerth et al., 2004; 101:7809–14). Chromatograms were exported from Leco ChromaTOF software (version 3.25) to R. Peak detection, retention time alignment, and library matching were obtained using the TargetSearch package from Bioconductor (Cuadros-Inostroza et al., BMC Bioinformatics. 2009;10:428). Metabolites were quantified by peak intensity of a selective mass, and metabolite intensities were normalized dividing by the sum of the total ion count followed by log2 transformation.","COLLISION_ENERGY":"70 eV"},

"MS_METABOLITE_DATA":{
"Units":"intensity log2",

"Data":[{"Metabolite":"Lactic Acid","SCC-9_1-EV intensity":"16.0921","SCC-9_2-EV intensity":"15.0396","SCC-9_3-EV intensity":"15.2940","SCC-9_4-EV intensity":"15.8178","SCC-9_5-EV intensity":"15.2035","LN1_1-EV intensity":"16.4507","LN1_2-EV intensity":"16.1413","LN1_3-EV intensity":"16.1772","LN1_4-EV intensity":"16.4353","LN1_5-EV intensity":"16.4956"},{"Metabolite":"Alanine","SCC-9_1-EV intensity":"13.1668","SCC-9_2-EV intensity":"12.8646","SCC-9_3-EV intensity":"13.0186","SCC-9_4-EV intensity":"12.8674","SCC-9_5-EV intensity":"13.2408","LN1_1-EV intensity":"14.4325","LN1_2-EV intensity":"13.8098","LN1_3-EV intensity":"14.2609","LN1_4-EV intensity":"13.4742","LN1_5-EV intensity":"13.8846"},{"Metabolite":"Pyruvate","SCC-9_1-EV intensity":"NA","SCC-9_2-EV intensity":"NA","SCC-9_3-EV intensity":"NA","SCC-9_4-EV intensity":"NA","SCC-9_5-EV intensity":"NA","LN1_1-EV intensity":"10.2393","LN1_2-EV intensity":"9.6254","LN1_3-EV intensity":"9.8553","LN1_4-EV intensity":"9.6397","LN1_5-EV intensity":"9.6110"},{"Metabolite":"Valine","SCC-9_1-EV intensity":"13.0952","SCC-9_2-EV intensity":"12.1752","SCC-9_3-EV intensity":"13.5783","SCC-9_4-EV intensity":"13.1598","SCC-9_5-EV intensity":"13.0098","LN1_1-EV intensity":"14.0988","LN1_2-EV intensity":"13.7853","LN1_3-EV intensity":"14.2176","LN1_4-EV intensity":"13.9546","LN1_5-EV intensity":"13.8121"},{"Metabolite":"Glycerol","SCC-9_1-EV intensity":"14.0373","SCC-9_2-EV intensity":"14.4563","SCC-9_3-EV intensity":"13.2972","SCC-9_4-EV intensity":"14.1792","SCC-9_5-EV intensity":"NA","LN1_1-EV intensity":"11.1201","LN1_2-EV intensity":"13.1929","LN1_3-EV intensity":"12.0184","LN1_4-EV intensity":"13.0211","LN1_5-EV intensity":"13.0238"},{"Metabolite":"L-Leucine","SCC-9_1-EV intensity":"12.8235","SCC-9_2-EV intensity":"12.0704","SCC-9_3-EV intensity":"13.2021","SCC-9_4-EV intensity":"12.9284","SCC-9_5-EV intensity":"12.7326","LN1_1-EV intensity":"13.7552","LN1_2-EV intensity":"13.5457","LN1_3-EV intensity":"13.9583","LN1_4-EV intensity":"13.6585","LN1_5-EV intensity":"13.5401"},{"Metabolite":"Isoleucine","SCC-9_1-EV intensity":"12.4242","SCC-9_2-EV intensity":"11.6299","SCC-9_3-EV intensity":"12.7996","SCC-9_4-EV intensity":"12.5180","SCC-9_5-EV intensity":"12.3602","LN1_1-EV intensity":"13.4868","LN1_2-EV intensity":"13.3125","LN1_3-EV intensity":"13.7442","LN1_4-EV intensity":"13.4141","LN1_5-EV intensity":"13.3334"},{"Metabolite":"Glycine","SCC-9_1-EV intensity":"12.8895","SCC-9_2-EV intensity":"11.8366","SCC-9_3-EV intensity":"12.3384","SCC-9_4-EV intensity":"13.4657","SCC-9_5-EV intensity":"13.2691","LN1_1-EV intensity":"13.0704","LN1_2-EV intensity":"12.9783","LN1_3-EV intensity":"13.0551","LN1_4-EV intensity":"12.2988","LN1_5-EV intensity":"12.3568"},{"Metabolite":"Phosphate","SCC-9_1-EV intensity":"17.2896","SCC-9_2-EV intensity":"17.3816","SCC-9_3-EV intensity":"17.1890","SCC-9_4-EV intensity":"17.0924","SCC-9_5-EV intensity":"17.0970","LN1_1-EV intensity":"14.9993","LN1_2-EV intensity":"16.1555","LN1_3-EV intensity":"15.7408","LN1_4-EV intensity":"15.9901","LN1_5-EV intensity":"16.0882"},{"Metabolite":"Urea","SCC-9_1-EV intensity":"9.7215","SCC-9_2-EV intensity":"NA","SCC-9_3-EV intensity":"9.4539","SCC-9_4-EV intensity":"11.7530","SCC-9_5-EV intensity":"11.9517","LN1_1-EV intensity":"8.1289","LN1_2-EV intensity":"NA","LN1_3-EV intensity":"8.5547","LN1_4-EV intensity":"10.8239","LN1_5-EV intensity":"10.6132"},{"Metabolite":"L-Serine","SCC-9_1-EV intensity":"10.0675","SCC-9_2-EV intensity":"11.3485","SCC-9_3-EV intensity":"10.6641","SCC-9_4-EV intensity":"10.9856","SCC-9_5-EV intensity":"NA","LN1_1-EV intensity":"11.5676","LN1_2-EV intensity":"11.4755","LN1_3-EV intensity":"11.6152","LN1_4-EV intensity":"11.3652","LN1_5-EV intensity":"11.2261"},{"Metabolite":"(-)-Riboflavin","SCC-9_1-EV intensity":"13.7394","SCC-9_2-EV intensity":"13.3399","SCC-9_3-EV intensity":"13.4336","SCC-9_4-EV intensity":"12.8848","SCC-9_5-EV intensity":"13.1643","LN1_1-EV intensity":"11.3187","LN1_2-EV intensity":"NA","LN1_3-EV intensity":"12.1027","LN1_4-EV intensity":"11.3587","LN1_5-EV intensity":"11.5578"},{"Metabolite":"L-Threonine","SCC-9_1-EV intensity":"11.5923","SCC-9_2-EV intensity":"10.9651","SCC-9_3-EV intensity":"12.0999","SCC-9_4-EV intensity":"11.5168","SCC-9_5-EV intensity":"11.6979","LN1_1-EV intensity":"13.2146","LN1_2-EV intensity":"12.7762","LN1_3-EV intensity":"13.1432","LN1_4-EV intensity":"12.5101","LN1_5-EV intensity":"12.4616"},{"Metabolite":"Uracil","SCC-9_1-EV intensity":"NA","SCC-9_2-EV intensity":"NA","SCC-9_3-EV intensity":"NA","SCC-9_4-EV intensity":"11.3522","SCC-9_5-EV intensity":"10.8951","LN1_1-EV intensity":"9.9931","LN1_2-EV intensity":"NA","LN1_3-EV intensity":"NA","LN1_4-EV intensity":"9.7885","LN1_5-EV intensity":"9.5520"},{"Metabolite":"Malic Acid","SCC-9_1-EV intensity":"7.3770","SCC-9_2-EV intensity":"7.0828","SCC-9_3-EV intensity":"7.5357","SCC-9_4-EV intensity":"NA","SCC-9_5-EV intensity":"NA","LN1_1-EV intensity":"6.7566","LN1_2-EV intensity":"6.3742","LN1_3-EV intensity":"6.7673","LN1_4-EV intensity":"7.0322","LN1_5-EV intensity":"6.4291"},{"Metabolite":"4-Aminobutyric Acid","SCC-9_1-EV intensity":"8.8249","SCC-9_2-EV intensity":"9.1759","SCC-9_3-EV intensity":"8.9203","SCC-9_4-EV intensity":"9.0028","SCC-9_5-EV intensity":"NA","LN1_1-EV intensity":"7.9740","LN1_2-EV intensity":"7.4800","LN1_3-EV intensity":"NA","LN1_4-EV intensity":"7.2634","LN1_5-EV intensity":"7.3775"},{"Metabolite":"L-Aspartic Acid","SCC-9_1-EV intensity":"9.7459","SCC-9_2-EV intensity":"10.2826","SCC-9_3-EV intensity":"9.9403","SCC-9_4-EV intensity":"NA","SCC-9_5-EV intensity":"NA","LN1_1-EV intensity":"10.0228","LN1_2-EV intensity":"9.7130","LN1_3-EV intensity":"10.3724","LN1_4-EV intensity":"9.9191","LN1_5-EV intensity":"9.8316"},{"Metabolite":"L-Methionine","SCC-9_1-EV intensity":"10.1815","SCC-9_2-EV intensity":"9.3633","SCC-9_3-EV intensity":"NA","SCC-9_4-EV intensity":"10.0725","SCC-9_5-EV intensity":"9.4452","LN1_1-EV intensity":"11.3230","LN1_2-EV intensity":"11.1869","LN1_3-EV intensity":"11.4892","LN1_4-EV intensity":"11.1116","LN1_5-EV intensity":"10.9769"},{"Metabolite":"Arginine","SCC-9_1-EV intensity":"10.2629","SCC-9_2-EV intensity":"10.0791","SCC-9_3-EV intensity":"NA","SCC-9_4-EV intensity":"10.8022","SCC-9_5-EV intensity":"10.2666","LN1_1-EV intensity":"12.7151","LN1_2-EV intensity":"12.3776","LN1_3-EV intensity":"12.5485","LN1_4-EV intensity":"11.8576","LN1_5-EV intensity":"11.5650"},{"Metabolite":"Pyroglutamic Acid","SCC-9_1-EV intensity":"13.5743","SCC-9_2-EV intensity":"13.0675","SCC-9_3-EV intensity":"14.4204","SCC-9_4-EV intensity":"14.4433","SCC-9_5-EV intensity":"14.5286","LN1_1-EV intensity":"14.6990","LN1_2-EV intensity":"14.2551","LN1_3-EV intensity":"14.9469","LN1_4-EV intensity":"13.9998","LN1_5-EV intensity":"13.7931"},{"Metabolite":"L-Glutamic Acid","SCC-9_1-EV intensity":"NA","SCC-9_2-EV intensity":"9.2882","SCC-9_3-EV intensity":"NA","SCC-9_4-EV intensity":"11.4346","SCC-9_5-EV intensity":"11.6617","LN1_1-EV intensity":"NA","LN1_2-EV intensity":"10.5948","LN1_3-EV intensity":"NA","LN1_4-EV intensity":"12.9167","LN1_5-EV intensity":"12.8924"},{"Metabolite":"Phenilalanine","SCC-9_1-EV intensity":"10.7977","SCC-9_2-EV intensity":"9.9155","SCC-9_3-EV intensity":"11.3620","SCC-9_4-EV intensity":"10.8972","SCC-9_5-EV intensity":"10.6564","LN1_1-EV intensity":"12.0021","LN1_2-EV intensity":"11.6883","LN1_3-EV intensity":"12.0355","LN1_4-EV intensity":"11.9314","LN1_5-EV intensity":"11.7805"},{"Metabolite":"D-Mannitol","SCC-9_1-EV intensity":"11.2935","SCC-9_2-EV intensity":"NA","SCC-9_3-EV intensity":"13.2577","SCC-9_4-EV intensity":"13.5748","SCC-9_5-EV intensity":"13.8864","LN1_1-EV intensity":"14.1459","LN1_2-EV intensity":"14.0782","LN1_3-EV intensity":"12.8636","LN1_4-EV intensity":"14.0171","LN1_5-EV intensity":"12.5683"},{"Metabolite":"Glucose","SCC-9_1-EV intensity":"11.7863","SCC-9_2-EV intensity":"10.6781","SCC-9_3-EV intensity":"12.4153","SCC-9_4-EV intensity":"11.9711","SCC-9_5-EV intensity":"11.4171","LN1_1-EV intensity":"13.6445","LN1_2-EV intensity":"12.8252","LN1_3-EV intensity":"13.3604","LN1_4-EV intensity":"12.8735","LN1_5-EV intensity":"12.7412"},{"Metabolite":"Citric Acid","SCC-9_1-EV intensity":"9.3271","SCC-9_2-EV intensity":"8.7663","SCC-9_3-EV intensity":"9.6340","SCC-9_4-EV intensity":"9.4327","SCC-9_5-EV intensity":"9.2548","LN1_1-EV intensity":"NA","LN1_2-EV intensity":"8.5483","LN1_3-EV intensity":"NA","LN1_4-EV intensity":"8.0222","LN1_5-EV intensity":"7.9275"},{"Metabolite":"Glutamine","SCC-9_1-EV intensity":"9.9959","SCC-9_2-EV intensity":"11.8413","SCC-9_3-EV intensity":"8.6882","SCC-9_4-EV intensity":"12.2040","SCC-9_5-EV intensity":"11.4274","LN1_1-EV intensity":"13.6464","LN1_2-EV intensity":"NA","LN1_3-EV intensity":"12.7043","LN1_4-EV intensity":"13.6671","LN1_5-EV intensity":"13.5215"},{"Metabolite":"L-Lysine","SCC-9_1-EV intensity":"11.3068","SCC-9_2-EV intensity":"11.0430","SCC-9_3-EV intensity":"12.0281","SCC-9_4-EV intensity":"12.0086","SCC-9_5-EV intensity":"11.3451","LN1_1-EV intensity":"13.4363","LN1_2-EV intensity":"13.1488","LN1_3-EV intensity":"13.3027","LN1_4-EV intensity":"13.1493","LN1_5-EV intensity":"12.9833"},{"Metabolite":"Myo-inositol","SCC-9_1-EV intensity":"10.3035","SCC-9_2-EV intensity":"9.2828","SCC-9_3-EV intensity":"10.8221","SCC-9_4-EV intensity":"10.3499","SCC-9_5-EV intensity":"10.2312","LN1_1-EV intensity":"11.4017","LN1_2-EV intensity":"11.1261","LN1_3-EV intensity":"11.3900","LN1_4-EV intensity":"11.0508","LN1_5-EV intensity":"11.0331"},{"Metabolite":"Tyrosine","SCC-9_1-EV intensity":"12.1325","SCC-9_2-EV intensity":"11.4047","SCC-9_3-EV intensity":"12.8047","SCC-9_4-EV intensity":"12.1618","SCC-9_5-EV intensity":"11.9136","LN1_1-EV intensity":"13.9590","LN1_2-EV intensity":"13.4521","LN1_3-EV intensity":"13.8175","LN1_4-EV intensity":"13.3924","LN1_5-EV intensity":"13.2678"},{"Metabolite":"Adenine","SCC-9_1-EV intensity":"NA","SCC-9_2-EV intensity":"NA","SCC-9_3-EV intensity":"NA","SCC-9_4-EV intensity":"9.6688","SCC-9_5-EV intensity":"9.0265","LN1_1-EV intensity":"8.4177","LN1_2-EV intensity":"NA","LN1_3-EV intensity":"NA","LN1_4-EV intensity":"8.1710","LN1_5-EV intensity":"7.7007"},{"Metabolite":"L-Histidine","SCC-9_1-EV intensity":"NA","SCC-9_2-EV intensity":"NA","SCC-9_3-EV intensity":"NA","SCC-9_4-EV intensity":"NA","SCC-9_5-EV intensity":"NA","LN1_1-EV intensity":"NA","LN1_2-EV intensity":"8.7549","LN1_3-EV intensity":"9.1321","LN1_4-EV intensity":"9.1387","LN1_5-EV intensity":"8.2306"},{"Metabolite":"Tryptophan","SCC-9_1-EV intensity":"9.1659","SCC-9_2-EV intensity":"8.8963","SCC-9_3-EV intensity":"9.0523","SCC-9_4-EV intensity":"8.8958","SCC-9_5-EV intensity":"NA","LN1_1-EV intensity":"9.8611","LN1_2-EV intensity":"8.5923","LN1_3-EV intensity":"9.2947","LN1_4-EV intensity":"9.2565","LN1_5-EV intensity":"9.1102"},{"Metabolite":"Sucrose","SCC-9_1-EV intensity":"11.1732","SCC-9_2-EV intensity":"11.0491","SCC-9_3-EV intensity":"8.6898","SCC-9_4-EV intensity":"10.0799","SCC-9_5-EV intensity":"11.5495","LN1_1-EV intensity":"7.5461","LN1_2-EV intensity":"NA","LN1_3-EV intensity":"8.1274","LN1_4-EV intensity":"8.7090","LN1_5-EV intensity":"8.4451"}],

"Metabolites":[{"metabolite_name":"Lactic Acid"},{"metabolite_name":"Alanine"},{"metabolite_name":"Pyruvate"},{"metabolite_name":"Valine"},{"metabolite_name":"Glycerol"},{"metabolite_name":"L-Leucine"},{"metabolite_name":"Isoleucine"},{"metabolite_name":"Glycine"},{"metabolite_name":"Phosphate"},{"metabolite_name":"Urea"},{"metabolite_name":"L-Serine"},{"metabolite_name":"(-)-Riboflavin"},{"metabolite_name":"L-Threonine"},{"metabolite_name":"Uracil"},{"metabolite_name":"Malic Acid"},{"metabolite_name":"4-Aminobutyric Acid"},{"metabolite_name":"L-Aspartic Acid"},{"metabolite_name":"L-Methionine"},{"metabolite_name":"Arginine"},{"metabolite_name":"Pyroglutamic Acid"},{"metabolite_name":"L-Glutamic Acid"},{"metabolite_name":"Phenilalanine"},{"metabolite_name":"D-Mannitol"},{"metabolite_name":"Glucose"},{"metabolite_name":"Citric Acid"},{"metabolite_name":"Glutamine"},{"metabolite_name":"L-Lysine"},{"metabolite_name":"Myo-inositol"},{"metabolite_name":"Tyrosine"},{"metabolite_name":"Adenine"},{"metabolite_name":"L-Histidine"},{"metabolite_name":"Tryptophan"},{"metabolite_name":"Sucrose"}]
}

}