{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001779","ANALYSIS_ID":"AN002888","VERSION":"1","CREATED_ON":"May 8, 2021, 11:06 am"},

"PROJECT":{"PROJECT_TYPE":"Untargeted quantitative metabolomics analysis","PROJECT_SUMMARY":"A549 lung cancer cells were treated in vitro with control, 0.5 mM ATP and 10 ng/ml TGF-beta for 2, 6 and 12 hours The untargeted metabolomics data was generated from these studies.","INSTITUTE":"Ohio University","DEPARTMENT":"Biological Sciences","LABORATORY":"Dr. Xiaozhuo Chen, Edison biotechnology Institute","FIRST_NAME":"Pratik","ADDRESS":"Room 425, Parks Hall, College of Pharmacy, Ohio State University, Columbus Ohio. 43210","EMAIL":"ps774614@ohio.edu","PHONE":"7406033801","FUNDING_SOURCE":"This study was funded by NIH R15 grant CA242177-01 to Dr. Xiaozhuo Chen.","PROJECT_TITLE":"Untargeted metabolomics analysis of A549 cancer cells treated in control, 0.5 mM ATP and 10 ng/ml TGF-beta for 2, 6 and 12 hours","LAST_NAME":"Shriwas"},

"STUDY":{"STUDY_TITLE":"Untargeted Metabolomics analysis of A549 treated with 0.5 mM extracellular ATP and 10 ng/ml TGF-beta","STUDY_SUMMARY":"Control, 0.5 mM extracellular ATP and 10 ng/ml TGF-beta were used to treated 5 million A549 lung cancer cells in vitro for 2, 6 and 12 hours. The untargeted metabolomics analysis was performed on the cell lysates. The main objective of the study was to determine changes in metabolite abundances in lung cancer after treatment with extracellular ATP and TGF-beta (a known EMT inducer).","INSTITUTE":"Ohio University","DEPARTMENT":"Biological Sciences","LABORATORY":"Dr. Xiaozhuo Chen, Edison biotechnology Institute","LAST_NAME":"Shriwas","FIRST_NAME":"Pratik","ADDRESS":"Room 425, Parks Hall, College of Pharmacy, Ohio State University, Columbus Ohio. 43210","EMAIL":"ps774614@ohio.edu","NUM_GROUPS":"7","STUDY_TYPE":"Untargeted metabolomics analysis in lung cancer cells","PHONE":"7406033801"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","CELL_BIOSOURCE_OR_SUPPLIER":"ATCC","CELL_STRAIN_DETAILS":"Human Lung epithelial A549 cancer cells","CELL_PASSAGE_NUMBER":"10","CELL_COUNTS":"5 million cells"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Control_1",
"Factors":{"Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"ControlA_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"Control_2",
"Factors":{"Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"ControlA_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"Control_3",
"Factors":{"Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"ControlB_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"Control_4",
"Factors":{"Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"ControlB_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h ATP_1",
"Factors":{"Treatment":"2h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"2hATP1_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h ATP_2",
"Factors":{"Treatment":"2h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"2hATP1_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h ATP_3",
"Factors":{"Treatment":"2h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"2hATP2_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h ATP_4",
"Factors":{"Treatment":"2h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"2hATP2_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h ATP_5",
"Factors":{"Treatment":"2h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"2hATP3_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h ATP_6",
"Factors":{"Treatment":"2h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"2hATP3_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"6h ATP_1",
"Factors":{"Treatment":"6h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"6hATP1_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"6h ATP_2",
"Factors":{"Treatment":"6h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"6hATP1_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"6h ATP_3",
"Factors":{"Treatment":"6h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"6hATP2_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"6h ATP_4",
"Factors":{"Treatment":"6h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"6hATP2_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h ATP_1",
"Factors":{"Treatment":"12h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"12hATP1_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h ATP_2",
"Factors":{"Treatment":"12h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"12hATP1_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h ATP_3",
"Factors":{"Treatment":"12h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"12hATP2_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h ATP_4",
"Factors":{"Treatment":"12h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"12hATP2_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h ATP_5",
"Factors":{"Treatment":"12h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"12hATP3_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h ATP_6",
"Factors":{"Treatment":"12h ATP"},
"Additional sample data":{"RAW_FILE_NAME":"12hATP3_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h TGF-β_1",
"Factors":{"Treatment":"2h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"2hTGF1_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h TGF-β_2",
"Factors":{"Treatment":"2h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"2hTGF1_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h TGF-β_3",
"Factors":{"Treatment":"2h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"2hTGF2_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h TGF-β_4",
"Factors":{"Treatment":"2h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"2hTGF2_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h TGF-β_5",
"Factors":{"Treatment":"2h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"2hTGF3_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"2h TGF-β_6",
"Factors":{"Treatment":"2h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"2hTGF3_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"6h TGF-β_1",
"Factors":{"Treatment":"6h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"6hTGF1_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"6h TGF-β_2",
"Factors":{"Treatment":"6h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"6hTGF1_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"6h TGF-β_3",
"Factors":{"Treatment":"6h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"6hTGF2_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"6h TGF-β_4",
"Factors":{"Treatment":"6h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"6hTGF2_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h TGF-β_1",
"Factors":{"Treatment":"12h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"12hTGF1_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h TGF-β_2",
"Factors":{"Treatment":"12h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"12hTGF1_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h TGF-β_3",
"Factors":{"Treatment":"12h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"12hTGF2_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h TGF-β_4",
"Factors":{"Treatment":"12h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"12hTGF2_2.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h TGF-β_5",
"Factors":{"Treatment":"12h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"12hTGF3_1.mzxml"}
},
{
"Subject ID":"-",
"Sample ID":"12h TGF-β_6",
"Factors":{"Treatment":"12h TGF-β"},
"Additional sample data":{"RAW_FILE_NAME":"12hTGF3_2.mzxml"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"A549 lung cancer cells were treated with control, ATP, TGF-beta and then collected according to sample preparation protocol.","COLLECTION_PROTOCOL_FILENAME":"Collection protocol","SAMPLE_TYPE":"Lung","COLLECTION_METHOD":"Ice cold methanol (80%) using cell scrappers","COLLECTION_LOCATION":"150mm dish","COLLECTION_FREQUENCY":"Once","COLLECTION_DURATION":"1-2 sec","VOLUMEORAMOUNT_COLLECTED":"1 ml","STORAGE_CONDITIONS":"-80℃","COLLECTION_VIALS":"Polypropylene 1.5 ml tubes","STORAGE_VIALS":"Polypropylene 1.5 ml","COLLECTION_TUBE_TEMP":"4C","TISSUE_CELL_QUANTITY_TAKEN":"5 million"},

"TREATMENT":{"TREATMENT_SUMMARY":"5 million A549 cells were treated with control (no treatment), 0.5 mM ATP or 10 ng/ml TGF-beta for 2, 6 and 12 hours.","TREATMENT":"In vitro treatment with small molecules","TREATMENT_DOSEVOLUME":"10 ml DMEM media containing appropriate compounds","CELL_STORAGE":"37C; 5% CO2 incubator","CELL_GROWTH_CONTAINER":"150 mm Dish tissue culture treated","CELL_MEDIA":"DMEM (10% FBS; 1% Pen/Strep)","CELL_HARVESTING":"after 2, 6 and 12 hours","CELL_PCT_CONFLUENCE":"80%","CELL_MEDIA_LASTCHANGED":"NA","TREATMENT_COMPOUND":"0.5 mM ATP and 10 ng/ml TGF-beta"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"5 × 106 A549 cells were treated with control (no treatment), 0.5 mM ATP or 10 ng/ml TGF-beta for 2, 6 and 12 hours. After treatment, cells were washed twice with deionized water and polar metabolites were then extracted with cryogenically cold 80% methanol/water mixture. LC-MS grade water, methanol, and acetonitrile (Fischer Scientific, PA, USA) were used. Methanol-extracted samples were then sonicated in cycles of sonication phase and rest phase for 10 minutes (5 second sonication phase and 10 seconds halt). The samples were then centrifuged at 13,000 rpm for 10 minutes and supernatant was then collected. Supernatants collected from in vitro and in vivo extraction were then lyophilized. Briefly, the supernatant was then lyophilized by using a speed vacuum evaporator. The samples were then dissolved into a mixture of acetonitrile/water (1:1; v/v).","SAMPLEPREP_PROTOCOL_FILENAME":"Sample preparation protocol","PROCESSING_METHOD":"CEll scrapping Quenching","PROCESSING_STORAGE_CONDITIONS":"-80℃","EXTRACTION_METHOD":"Quenching with Ice cold methanol","EXTRACT_ENRICHMENT":"Speed vaccum evaporator","EXTRACT_STORAGE":"-80℃","SAMPLE_RESUSPENSION":"Acetonitrile/water (1:1)"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"The entire LC/MS-MS experiment was performed in the Campus Chemical Instrumentation Center’s Mass Spectrometry and Proteomics facility at The Ohio State University. Lyophilized samples were dissolved in equal amounts of LC-MS grade water and acetonitrile and run with LC/MS-MS analysis, using an untargeted metabolomics approach by utilizing Agilent Q-TOF 6545 mass spectrometer connected to an Agilent 1290 UHPLC system with a Poroshell 120 SB-C18 (2 x 100 mm, 2.7 µm particle size) column. The LC gradient consisted of solvent A, H2O with 0.1 % Formic acid, and solvent B, 100 % acetonitrile at a 200 µL/min flow rate with an initial 2 % solvent B with a linear ramp to 95 % B at 15 min, holding at 95% B for 1 minutes, and back to 2 % B from 16 min and equilibration of 2 % B until min 32. A 5 µL volume sample was injected for each run and the top 5 ions were selected for data-dependent analysis with a 15 second exclusion window.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1290","COLUMN_NAME":"Poroshell 120 SB-C18 (2.1 x 100 mm x 2.7 µm)","FLOW_GRADIENT":"an initial 2 % solvent B with a linear ramp to 95 % B at 15 min, holding at 95% B for 1 minutes, and back to 2 % B from 16 min and equilibration of 2 % B until min 32.","FLOW_RATE":"200 µL/min","COLUMN_TEMPERATURE":"40","SOLVENT_A":"H2O with 0.1 % Formic acid","SOLVENT_B":"100 % acetonitrile"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Campus Center for Chemical Instrumentation (CCIC)"},

"MS":{"INSTRUMENT_NAME":"Agilent 6545 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Masshunter software was used to collect the raw data. Progenesis was used for peak intergration."},

"MS_METABOLITE_DATA":{
"Units":"normalized",

"Data":[{"Metabolite":"glucose-6-phosphate","Control_1":"102979.3174","Control_2":"95099.67725","Control_3":"169225.4087","Control_4":"194364.2848","2h ATP_1":"142345.3552","2h ATP_2":"123460.2078","2h ATP_3":"123023.109","2h ATP_4":"119684.3112","2h TGF-β_1":"59423.01716","2h TGF-β_2":"52552.15518","2h TGF-β_3":"57348.77976","2h TGF-β_4":"66727.4978"},{"Metabolite":"gamma-Glutamylcysteine","Control_1":"106200.9029","Control_2":"111657.9312","Control_3":"145486.5678","Control_4":"160580.0895","2h ATP_1":"28451.09293","2h ATP_2":"32751.30622","2h ATP_3":"58632.56987","2h ATP_4":"47255.0099","2h TGF-β_1":"4651.684556","2h TGF-β_2":"3226.595579","2h TGF-β_3":"4912.753233","2h TGF-β_4":"3554.641448"},{"Metabolite":"L-Cysteine","Control_1":"","Control_2":"143279.0567","Control_3":"139255.5089","Control_4":"229898.7066","2h ATP_1":"97083.09029","2h ATP_2":"36521.82124","2h ATP_3":"86218.81787","2h ATP_4":"46444.96421","2h TGF-β_1":"12387.98658","2h TGF-β_2":"11749.49594","2h TGF-β_3":"17776.8443","2h TGF-β_4":"17987.98454"},{"Metabolite":"NADPH","Control_1":"899.3351172","Control_2":"3250.756112","Control_3":"2149.439816","Control_4":"906.8343734","2h ATP_1":"1553.171532","2h ATP_2":"2322.815674","2h ATP_3":"1020.819744","2h ATP_4":"2858.617641","2h TGF-β_1":"1234.217026","2h TGF-β_2":"1469.710669","2h TGF-β_3":"1342.879962","2h TGF-β_4":"1726.900041"},{"Metabolite":"NADP","Control_1":"25430.75568","Control_2":"35891.25176","Control_3":"27447.82833","Control_4":"17634.48117","2h ATP_1":"34987.29924","2h ATP_2":"33126.94661","2h ATP_3":"22456.62328","2h ATP_4":"29629.96277","2h TGF-β_1":"22232.23451","2h TGF-β_2":"24060.41113","2h TGF-β_3":"23148.89253","2h TGF-β_4":"21383.44907"},{"Metabolite":"Ubiquinone-2","Control_1":"957.9189286","Control_2":"1036.762959","Control_3":"437.9648284","Control_4":"","2h ATP_1":"377.1326976","2h ATP_2":"278.1060856","2h ATP_3":"475.9302029","2h ATP_4":"628.114387","2h TGF-β_1":"433.0856195","2h TGF-β_2":"447.4160761","2h TGF-β_3":"362.6247398","2h TGF-β_4":"658.8495932"},{"Metabolite":"QH(2)","Control_1":"17105.33318","Control_2":"7099.353003","Control_3":"","Control_4":"29271.29429","2h ATP_1":"41445.37858","2h ATP_2":"39683.52244","2h ATP_3":"75805.15361","2h ATP_4":"61366.16093","2h TGF-β_1":"4228.279119","2h TGF-β_2":"3585.672182","2h TGF-β_3":"2148.902116","2h TGF-β_4":"6774.171271"},{"Metabolite":"Guanosine triphosphate","Control_1":"1119.444524","Control_2":"899.0160947","Control_3":"496.2612172","Control_4":"501.994448","2h ATP_1":"805.5061377","2h ATP_2":"459.8842034","2h ATP_3":"395.8217624","2h ATP_4":"236.0024761","2h TGF-β_1":"6307.963919","2h TGF-β_2":"8525.178851","2h TGF-β_3":"8502.192867","2h TGF-β_4":"6208.768129"},{"Metabolite":"NADH","Control_1":"12983.28892","Control_2":"15231.76412","Control_3":"8204.008583","Control_4":"15335.04874","2h ATP_1":"16455.91694","2h ATP_2":"18838.24167","2h ATP_3":"21647.6","2h ATP_4":"20259.1694","2h TGF-β_1":"17904.41933","2h TGF-β_2":"16249.72582","2h TGF-β_3":"20410.58666","2h TGF-β_4":"33012.77186"},{"Metabolite":"FADH","Control_1":"1770.178308","Control_2":"4238.917001","Control_3":"1347.455445","Control_4":"1704.475493","2h ATP_1":"1936.681228","2h ATP_2":"1244.248591","2h ATP_3":"2704.605031","2h ATP_4":"3115.99094","2h TGF-β_1":"5832.545752","2h TGF-β_2":"11748.24209","2h TGF-β_3":"5871.909317","2h TGF-β_4":"2083.517754"},{"Metabolite":"Sphingosine 1-phosphate","Control_1":"","Control_2":"44709.64106","Control_3":"59937.90217","Control_4":"48113.36454","2h ATP_1":"","2h ATP_2":"111886.0248","2h ATP_3":"77291.01023","2h ATP_4":"117531.4062","2h TGF-β_1":"","2h TGF-β_2":"101045.3927","2h TGF-β_3":"108943.7019","2h TGF-β_4":"82055.12193"},{"Metabolite":"Sphingosine","Control_1":"","Control_2":"370904.4219","Control_3":"1599826.709","Control_4":"236812.8422","2h ATP_1":"","2h ATP_2":"189881.6439","2h ATP_3":"113495.854","2h ATP_4":"104878.4702","2h TGF-β_1":"","2h TGF-β_2":"184609.9763","2h TGF-β_3":"260548.8452","2h TGF-β_4":"195231.089"},{"Metabolite":"Sphinganine","Control_1":"","Control_2":"17326.17368","Control_3":"27034.60726","Control_4":"6836.958729","2h ATP_1":"","2h ATP_2":"4100.10731","2h ATP_3":"4581.469327","2h ATP_4":"5019.052169","2h TGF-β_1":"","2h TGF-β_2":"985.6085801","2h TGF-β_3":"3852.990169","2h TGF-β_4":"2983.474302"},{"Metabolite":"Sphinganine 1-phosphate","Control_1":"","Control_2":"15565.27036","Control_3":"15868.32095","Control_4":"14969.36669","2h ATP_1":"","2h ATP_2":"31171.96214","2h ATP_3":"31943.50697","2h ATP_4":"33673.36749","2h TGF-β_1":"","2h TGF-β_2":"17924.35933","2h TGF-β_3":"19093.36799","2h TGF-β_4":"24000.93178"},{"Metabolite":"Choline","Control_1":"","Control_2":"787.8240036","Control_3":"1621.194477","Control_4":"3767.779458","2h ATP_1":"","2h ATP_2":"3550.339187","2h ATP_3":"3559.402373","2h ATP_4":"3232.930367","2h TGF-β_1":"","2h TGF-β_2":"1166.68219","2h TGF-β_3":"1245.251183","2h TGF-β_4":"1991.634849"},{"Metabolite":"PGP(16:0/16:0)","Control_1":"","Control_2":"3620.624188","Control_3":"4532.942908","Control_4":"5849.090226","2h ATP_1":"","2h ATP_2":"52411.56384","2h ATP_3":"11081.43711","2h ATP_4":"16621.27282","2h TGF-β_1":"","2h TGF-β_2":"40891.70646","2h TGF-β_3":"16142.49826","2h TGF-β_4":"36780.25224"},{"Metabolite":"CDP-DG(16:0/16:0)","Control_1":"","Control_2":"8832.547528","Control_3":"4773.215017","Control_4":"5241.315741","2h ATP_1":"","2h ATP_2":"2795.827532","2h ATP_3":"2653.382588","2h ATP_4":"3571.393825","2h TGF-β_1":"","2h TGF-β_2":"1792.02473","2h TGF-β_3":"2180.495334","2h TGF-β_4":"848.5393192"},{"Metabolite":"CerP(d18:1/12:0)","Control_1":"","Control_2":"774.5729885","Control_3":"322.0608539","Control_4":"637.0527935","2h ATP_1":"","2h ATP_2":"1317.925062","2h ATP_3":"1135.041644","2h ATP_4":"932.1830646","2h TGF-β_1":"","2h TGF-β_2":"673.9284138","2h TGF-β_3":"1430.47275","2h TGF-β_4":"696.0295746"},{"Metabolite":"Dihydroceramide","Control_1":"","Control_2":"67780.53513","Control_3":"29793.09816","Control_4":"38044.23758","2h ATP_1":"","2h ATP_2":"49263.54581","2h ATP_3":"38499.07602","2h ATP_4":"45912.79527","2h TGF-β_1":"","2h TGF-β_2":"87707.31406","2h TGF-β_3":"67813.52302","2h TGF-β_4":"61678.82656"},{"Metabolite":"Arachidonyl-CoA","Control_1":"","Control_2":"509.2674814","Control_3":"678.541006","Control_4":"437.0566543","2h ATP_1":"","2h ATP_2":"3601.950809","2h ATP_3":"2806.106393","2h ATP_4":"1516.036512","2h TGF-β_1":"","2h TGF-β_2":"2131.086522","2h TGF-β_3":"2984.53387","2h TGF-β_4":"3379.96452"}],

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}

}