{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001862","ANALYSIS_ID":"AN003019","VERSION":"1","CREATED_ON":"July 17, 2021, 9:25 am"},

"PROJECT":{"PROJECT_TITLE":"Cross-feeding between intestinal pathobionts promotes their overgrowth during undernutrition","PROJECT_SUMMARY":"Child undernutrition is a global health issue associated with a high burden of infectious disease. Undernourished children display an overabundance of intestinal pathogens and pathobionts, and these bacteria induce enteric dysfunction in undernourished mice; however, the cause of their overgrowth remains poorly defined. Here, we show that disease-inducing human isolates of Enterobacteriaceae and Bacteroidales spp. are capable of multi-species symbiotic cross-feeding, resulting in synergistic growth of a mixed community in vitro. Growth synergy occurs uniquely under malnourished conditions limited in protein and iron: in this context, Bacteroidales spp. liberate diet- and mucin-derived sugars and Enterobacteriaceae spp. enhance the bioavailability of iron. Analysis of human microbiota datasets reveals that Bacteroidaceae and Enterobacteriaceae are strongly correlated in undernourished children, but not in adequately nourished children, consistent with a diet-dependent growth synergy in the human gut. Together these data suggest that dietary cross-feeding fuels the overgrowth of pathobionts in undernutrition.","INSTITUTE":"University of British Columbia","DEPARTMENT":"Michael Smith Laboratories","LAST_NAME":"Huus","FIRST_NAME":"Kelsey","ADDRESS":"3125 East Mall, Vancouver, British Columbia, V6T 1Z4, Canada","EMAIL":"khuus@msl.ubc.ca","PHONE":"+1-604-822-2210"},

"STUDY":{"STUDY_TITLE":"Cross-feeding between intestinal pathobionts promotes their overgrowth during undernutrition","STUDY_SUMMARY":"Child undernutrition is a global health issue associated with a high burden of infectious disease. Undernourished children display an overabundance of intestinal pathogens and pathobionts, and these bacteria induce enteric dysfunction in undernourished mice; however, the cause of their overgrowth remains poorly defined. Here, we show that disease-inducing human isolates of Enterobacteriaceae and Bacteroidales spp. are capable of multi-species symbiotic cross-feeding, resulting in synergistic growth of a mixed community in vitro. Growth synergy occurs uniquely under malnourished conditions limited in protein and iron: in this context, Bacteroidales spp. liberate diet- and mucin-derived sugars and Enterobacteriaceae spp. enhance the bioavailability of iron. Analysis of human microbiota datasets reveals that Bacteroidaceae and Enterobacteriaceae are strongly correlated in undernourished children, but not in adequately nourished children, consistent with a diet-dependent growth synergy in the human gut. Together these data suggest that dietary cross-feeding fuels the overgrowth of pathobionts in undernutrition.","INSTITUTE":"University of British Columbia","DEPARTMENT":"Michael Smith Laboratories","LAST_NAME":"Huus","FIRST_NAME":"Kelsey","ADDRESS":"3125 East Mall","EMAIL":"khuus@msl.ubc.ca","PHONE":"+1-604-822-2210"},

"SUBJECT":{"SUBJECT_TYPE":"Bacteria","SUBJECT_SPECIES":"Bacteroides spp. and Escherichia spp. (mixed communities)","TAXONOMY_ID":"B. fragilis 3_1_12; B. vulgatus 3_1_40A; B. ovatus 3_8_47; B. dorei 5_1_36; P. distasonis 2_1_33B; E. coli 3_1_53; E. coli 4_1_47"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"01_B1-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"01_B1-16h.d","Replicate":"1","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"02_B2-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"02_B2-16h.d","Replicate":"2","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"03_B3-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"03_B3-16h.d","Replicate":"3","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"04_BO1-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"04_BO1-16h.d","Replicate":"1","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"05_BO2-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"05_BO2-16h.d","Replicate":"2","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"06_BO3-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"06_BO3-16h.d","Replicate":"3","Analyte":"Sugars"}
},
{
"Subject ID":"E_coli",
"Sample ID":"07_E1-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"07_E1-16h.d","Replicate":"1","Analyte":"Sugars"}
},
{
"Subject ID":"E_coli",
"Sample ID":"08_E2-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"08_E2-16h.d","Replicate":"2","Analyte":"Sugars"}
},
{
"Subject ID":"E_coli",
"Sample ID":"09_E3-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"09_E3-16h.d","Replicate":"3","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"10_BE1-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"10_BE1-16h.d","Replicate":"1","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"11_BE2-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"11_BE2-16h.d","Replicate":"2","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"12_BE3-16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"12_BE3-16h.d","Replicate":"3","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"13_B1-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"13_B1-24h.d","Replicate":"1","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"14_B2-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"14_B2-24h.d","Replicate":"2","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"15_B3-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"15_B3-24h.d","Replicate":"3","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"16_BO1-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"16_BO1-24h.d","Replicate":"1","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"17_BO2-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"17_BO2-24h.d","Replicate":"2","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"18_BO3-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"18_BO3-24h.d","Replicate":"3","Analyte":"Sugars"}
},
{
"Subject ID":"E_coli",
"Sample ID":"19_E1-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"19_E1-24h.d","Replicate":"1","Analyte":"Sugars"}
},
{
"Subject ID":"E_coli",
"Sample ID":"20_E2-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"20_E2-24h.d","Replicate":"2","Analyte":"Sugars"}
},
{
"Subject ID":"E_coli",
"Sample ID":"21_E3-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"21_E3-24h.d","Replicate":"3","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"22_BE1-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"22_BE1-24h.d","Replicate":"1","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"23_BE2-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"23_BE2-24h.d","Replicate":"2","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"24_BE3-24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"24_BE3-24h.d","Replicate":"3","Analyte":"Sugars"}
},
{
"Subject ID":"Blank",
"Sample ID":"25_Blank1",
"Factors":{"Factor":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"25_Blank1.d","Replicate":"1","Analyte":"Sugars"}
},
{
"Subject ID":"Blank",
"Sample ID":"26_Blank2",
"Factors":{"Factor":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"26_Blank2.d","Replicate":"2","Analyte":"Sugars"}
},
{
"Subject ID":"Blank",
"Sample ID":"27_Blank3",
"Factors":{"Factor":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"27_Blank3.d","Replicate":"3","Analyte":"Sugars"}
},
{
"Subject ID":"Blank",
"Sample ID":"28_Blank4",
"Factors":{"Factor":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"28_Blank4.d","Replicate":"4","Analyte":"Sugars"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"B4_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"B4_16H_OK.d","Replicate":"4","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"B5_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"B5_16H_3.d","Replicate":"4","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"B6_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"B6_16H_3.d","Replicate":"4","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"BO4_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"B04_16H_OK.d","Replicate":"4","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"BO5_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"B05_16H_3.d","Replicate":"4","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"BO6_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"B06_16H_3.d","Replicate":"4","Analyte":"SCFA"}
},
{
"Subject ID":"E_coli",
"Sample ID":"E4_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"E4_16H_OK.d","Replicate":"4","Analyte":"SCFA"}
},
{
"Subject ID":"E_coli",
"Sample ID":"E5_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"E5_16H_3.d","Replicate":"4","Analyte":"SCFA"}
},
{
"Subject ID":"E_coli",
"Sample ID":"E6_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"E6_16H_3.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"BE4_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"BE4_16H_OK.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"BE5_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"BE5_16H_3.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"BE6_16h",
"Factors":{"Factor":"16h"},
"Additional sample data":{"RAW_FILE_NAME":"BE6_16H_3.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"B4_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"B4_24H.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"B5_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"B5_24H_3.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_mix",
"Sample ID":"B6_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"B6_24H_3.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"BO4_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"B04_24H.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"BO5_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"B05_24H_3.d","Replicate":"6","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroides_ovatus",
"Sample ID":"BO6_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"B06_24H_3.d","Replicate":"6","Analyte":"SCFA"}
},
{
"Subject ID":"E_coli",
"Sample ID":"E4_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"E4_24H.d","Replicate":"6","Analyte":"SCFA"}
},
{
"Subject ID":"E_coli",
"Sample ID":"E5_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"E5_24H_3.d","Replicate":"6","Analyte":"SCFA"}
},
{
"Subject ID":"E_coli",
"Sample ID":"E6_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"E6_16H_3.d","Replicate":"6","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"BE4_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"BE4_24H.d","Replicate":"6","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"BE5_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"BE5_24H_3.d","Replicate":"6","Analyte":"SCFA"}
},
{
"Subject ID":"Bacteroidales_Ecoli_mix",
"Sample ID":"BE6_24h",
"Factors":{"Factor":"24h"},
"Additional sample data":{"RAW_FILE_NAME":"BE6_24H_3.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Blank",
"Sample ID":"blank_media1",
"Factors":{"Factor":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"blank_3.d","Replicate":"4","Analyte":"SCFA"}
},
{
"Subject ID":"Blank",
"Sample ID":"blank_media2",
"Factors":{"Factor":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"blank2_3.d","Replicate":"5","Analyte":"SCFA"}
},
{
"Subject ID":"Blank",
"Sample ID":"blank_media3",
"Factors":{"Factor":"0h"},
"Additional sample data":{"RAW_FILE_NAME":"BLANK4_OK.d","Replicate":"6","Analyte":"SCFA"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Bacteria were grown anaerobically at 37ºC in MAL-M medium. Culture supernatants at 0, 16 and 24h were collected by centrifugation at 16000 g for 20 minutes. Supernatants were filter sterilized at 0.22 µM and stored at -70ºC before analysis.","SAMPLE_TYPE":"Bacterial cells","STORAGE_CONDITIONS":"Described in summary"},

"TREATMENT":{"TREATMENT_SUMMARY":"The groups differ by bacterial community composition as follows: B, Bacteroidales mix (B. fragilis, B. vulgatus B. ovatus, B. dorei, P. distasonis); E, E. coli mix (E. coli 3_1_53 and E. coli 4_1_47); BE, Bacteroidales-E.coli mix (all seven strains as above); BO, B. ovatus only. Strains were inoculated in equal proportions based on normalized O.D. from overnight input cultures."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Sugars Analysis Low molecular weight sugars and NAc-sugar amines were quantified by LC-MRM/MS at The Metabolomics Innovation Centre (TMIC) commercial service (University of Victoria , Genome BC Proteomics Centre), according to previously published UPLC-MRM/MS methods (Han et al 2016, Electrophoresis), with necessary modifications. In brief, a mixed stock solution of 13 low-MW sugars and 4 N-acetyl sugar amines was prepared with the use of their standard substances in 80% methanol at 500µM for each compound. This solution was then serially diluted with the same solvent to prepare calibration solutions in a concentration range of 0.002 to 125µM. For chemical derivatization, 20µL of each medium sample or each calibration solution was mixed in turn with 20µL of an internal standard solution containing 13C6-glucose, 13C6-mannose, 13C6-fructose and 13C5-ribose in water, 40µL of 200-mM 3-nitrophenylhydrazine hydrochloride solution in 60% methanol and 40µL of 200-mM EDC.HCl solution prepared in a mixed solvent of methanol/water/pyridine (60:40:5, v/v/v). The mixture was allowed to react at 50ºC for 90 min. SCFA Analysis Quantification of short-chain fatty acids was performed in-house according to a method developed by Han et al., with minor modifications(Han et al 2015, Analytica Chimica Acta). Briefly, 500µL of supernatant were mixed with 500µL of 50 % acetonitrile, then the mixture was vortexed for 5 minutes and centrifuged at 7000 x g for 5 minutes. 50 µL of the organic phase were derivatized adding 20µL of 200mM 3NPH in 50 % acetonitrile and 20 µL 120 mM EDC solution of 6 % pyridine in 50 % acetonitrile. The mixture was left under agitation at 40ºC for 30 minutes. After this time reaction was stopped adding 100 µL of 0.1 % formic acid in 90 % acetonitrile."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"SCFA Analysis","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1200","COLUMN_NAME":"Agilent Zorbax 300 C18 (250x4.6mm)","METHODS_FILENAME":"methods_summary_ms.docx"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 6460 QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"A collision energy of 10V was used for multiple reaction monitoring (MRM), and LC-MS/MS data were analysed by Mass Hunter Qualitative Analysis B.06.00 software (Agilent Technologies). The identification and quantification of the SCFAs were carried out based on the retention time and mass fragmentation pattern comparing with standards. Six-point calibration curves made by peak area vs concentration of the pure standards were used to quantify the different SCFA. The linearity of the curves was determined by the coefficient of determination (R2), being higher than 0.99 for all standards. Concentrations of the SCFAs were calculated by interpolating the calibration curves of individual compounds."},

"MS_METABOLITE_DATA":{
"Units":"µM",

"Data":[{"Metabolite":"lactic acid","B4_16h":"0.216319416","B4_24h":"0.418668411","B5_16h":"0.303744653","B5_24h":"0.615087838","B6_16h":"0.285882789","B6_24h":"0.590861276","BE4_16h":"0.19137349","BE4_24h":"0.02518339","BE5_16h":"0.266537968","BE5_24h":"0.493279732","BE6_16h":"0.279849295","BE6_24h":"0.549332319","BO4_16h":"0.135810055","BO4_24h":"1.674187613","BO5_16h":"0.155498577","BO5_24h":"1.771797891","BO6_16h":"0.264811608","BO6_24h":"2.173965954","E4_16h":"0.013144378","E4_24h":"0.416259143","E5_16h":"0.052295855","E5_24h":"0.059590868","E6_16h":"0.065745113","E6_24h":"0.07129208"},{"Metabolite":"acetic acid","B4_16h":"0.74411919","B4_24h":"1.57920435","B5_16h":"0.989406328","B5_24h":"1.836486778","B6_16h":"0.873085635","B6_24h":"1.781273609","BE4_16h":"1.748542845","BE4_24h":"0.653692091","BE5_16h":"2.22383907","BE5_24h":"3.863670777","BE6_16h":"1.603206277","BE6_24h":"3.759617025","BO4_16h":"0.374867659","BO4_24h":"1.505910161","BO5_16h":"0.372199829","BO5_24h":"1.553801423","BO6_16h":"0.747727462","BO6_24h":"1.886878445","E4_16h":"0.253109441","E4_24h":"3.605446462","E5_16h":"0.416802039","E5_24h":"0.654555461","E6_16h":"0.404385706","E6_24h":"0.648794291"},{"Metabolite":"propionic acid","B4_16h":"0.451605923","B4_24h":"1.309627347","B5_16h":"0.615456023","B5_24h":"1.468421931","B6_16h":"0.487008961","B6_24h":"1.402489588","BE4_16h":"0.897122871","BE4_24h":"0.08918136","BE5_16h":"1.125763006","BE5_24h":"1.669607071","BE6_16h":"1.081934774","BE6_24h":"1.519170725","BO4_16h":"0.080765955","BO4_24h":"0.876525888","BO5_16h":"0.067346729","BO5_24h":"0.801903089","BO6_16h":"0.165274511","BO6_24h":"0.980996107","E4_16h":"0.043658204","E4_24h":"1.575518629","E5_16h":"0.085026975","E5_24h":"0.088498554","E6_16h":"0.083639819","E6_24h":"0.091203232"},{"Metabolite":"isobutiric acid","B4_16h":"0.003129913","B4_24h":"0.012125603","B5_16h":"0.002337934","B5_24h":"0.01180457","B6_16h":"0.003095037","B6_24h":"0.011102356","BE4_16h":"0.004731226","BE4_24h":"1.94364E-05","BE5_16h":"0.005269822","BE5_24h":"0.009056482","BE6_16h":"0.0048503","BE6_24h":"0.007587345","BO4_16h":"0","BO4_24h":"0.002299794","BO5_16h":"0.000249466","BO5_24h":"0.001521177","BO6_16h":"0","BO6_24h":"0.002200253","E4_16h":"0","E4_24h":"0.010210403","E5_16h":"0","E5_24h":"0.000236845","E6_16h":"0","E6_24h":"0.000168933"}],

"Metabolites":[{"metabolite_name":"lactic acid"},{"metabolite_name":"acetic acid"},{"metabolite_name":"propionic acid"},{"metabolite_name":"isobutiric acid"}]
}

}