{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001997","ANALYSIS_ID":"AN003257","VERSION":"1","CREATED_ON":"November 22, 2021, 12:06 pm"},

"PROJECT":{"PROJECT_TITLE":"Polyamine import and accumulation causes immunomodulation in macrophages engulfing apoptotic cells","PROJECT_SUMMARY":"Phagocytosis of apoptotic cells, termed efferocytosis, is critical for tissue homeostasis and drives anti-inflammatory programming in engulfing macrophages. Here, we assess metabolites in naïve and inflammatory macrophages following engulfment of multiple cellular and non-cellular targets. Efferocytosis leads to unique increases in the arginine-derived polyamines, spermidine and spermine, in vitro and in vivo. Surprisingly, polyamine accumulation after efferocytosis does not arise from retention of apoptotic cell metabolites or de novo synthesis, but from enhanced polyamine import that is dependent on Rac1, actin, and PI3 kinase. Blocking polyamine import prevents efferocytosis from suppressing macrophage IL-1or IL-6. This identifies efferocytosis as a trigger for polyamine import and accumulation, and imported polyamines as mediators of efferocytosis-induced immune reprogramming.","INSTITUTE":"University of Colorado Denver","LAST_NAME":"Haines","FIRST_NAME":"Julie","ADDRESS":"12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA","EMAIL":"julie.haines@cuanschutz.edu","PHONE":"3037243339"},

"STUDY":{"STUDY_TITLE":"Polyamine import and accumulation causes immunomodulation in macrophages engulfing apoptotic cells (Part 2)","STUDY_SUMMARY":"Phagocytosis of apoptotic cells, termed efferocytosis, is critical for tissue homeostasis and drives anti-inflammatory programming in engulfing macrophages. Here, we assess metabolites in naïve and inflammatory macrophages following engulfment of multiple cellular and non-cellular targets. Efferocytosis leads to unique increases in the arginine-derived polyamines, spermidine and spermine, in vitro and in vivo. Surprisingly, polyamine accumulation after efferocytosis does not arise from retention of apoptotic cell metabolites or de novo synthesis, but from enhanced polyamine import that is dependent on Rac1, actin, and PI3 kinase. Blocking polyamine import prevents efferocytosis from suppressing macrophage IL-1or IL-6. This identifies efferocytosis as a trigger for polyamine import and accumulation, and imported polyamines as mediators of efferocytosis-induced immune reprogramming.","INSTITUTE":"University of Colorado Denver","LAST_NAME":"Haines","FIRST_NAME":"Julie","ADDRESS":"12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA","EMAIL":"julie.haines@cuanschutz.edu","PHONE":"3037243339"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"4h 1",
"Factors":{"category":"4h unexposed"},
"Additional sample data":{"RAW_FILE_NAME":"37"}
},
{
"Subject ID":"-",
"Sample ID":"4h 2",
"Factors":{"category":"4h unexposed"},
"Additional sample data":{"RAW_FILE_NAME":"40"}
},
{
"Subject ID":"-",
"Sample ID":"4h 3",
"Factors":{"category":"4h unexposed"},
"Additional sample data":{"RAW_FILE_NAME":"43"}
},
{
"Subject ID":"-",
"Sample ID":"4h 1-",
"Factors":{"category":"4h non-engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"38"}
},
{
"Subject ID":"-",
"Sample ID":"4h 2-",
"Factors":{"category":"4h non-engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"41"}
},
{
"Subject ID":"-",
"Sample ID":"4h 3-",
"Factors":{"category":"4h non-engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"44"}
},
{
"Subject ID":"-",
"Sample ID":"4h 1+",
"Factors":{"category":"4h engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"39"}
},
{
"Subject ID":"-",
"Sample ID":"4h 2+",
"Factors":{"category":"4h engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"42"}
},
{
"Subject ID":"-",
"Sample ID":"4h 3+",
"Factors":{"category":"4h engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"45"}
},
{
"Subject ID":"-",
"Sample ID":"8h 1",
"Factors":{"category":"8h unexposed"},
"Additional sample data":{"RAW_FILE_NAME":"46"}
},
{
"Subject ID":"-",
"Sample ID":"8h 2",
"Factors":{"category":"8h unexposed"},
"Additional sample data":{"RAW_FILE_NAME":"49"}
},
{
"Subject ID":"-",
"Sample ID":"8h 3",
"Factors":{"category":"8h unexposed"},
"Additional sample data":{"RAW_FILE_NAME":"52"}
},
{
"Subject ID":"-",
"Sample ID":"8h 1-",
"Factors":{"category":"8h non-engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"47"}
},
{
"Subject ID":"-",
"Sample ID":"8h 2-",
"Factors":{"category":"8h non-engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"50"}
},
{
"Subject ID":"-",
"Sample ID":"8h 3-",
"Factors":{"category":"8h non-engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"53"}
},
{
"Subject ID":"-",
"Sample ID":"8h 1+",
"Factors":{"category":"8h engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"48"}
},
{
"Subject ID":"-",
"Sample ID":"8h 2+",
"Factors":{"category":"8h engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"51"}
},
{
"Subject ID":"-",
"Sample ID":"8h 3+",
"Factors":{"category":"8h engulfing"},
"Additional sample data":{"RAW_FILE_NAME":"54"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Experimental animals This study was approved and performed in accordance with the ethical guidelines of the Institutional Animal Care and Use Committee at National Jewish Health. C57BL/6J (000664), B6-CD45.1 (002014) and CCR2KO (004999) mice were obtained from Jackson Laboratories (Bar Harbor, ME, USA). DsRed.T3 mice (available as 006051 at Jackson Labs) were generously shared by the Nagy laboratory (Vintersten et al., 2004). CCR2KO mice were crossed with DsRed mice until double homozygous. Mice used in experiments were between 8-16 weeks of age. Both male and female sex were used in experiments except bone marrow chimera experiments, which used only male mice. Human tissue This study used deidentified donor peripheral blood mononuclear cells (PBMC) collected and isolated (Young et al., 2011) by the National Jewish Blood Prep Core (IRB No. HS-1285). Age and sex of deidentified blood donors in this study is not known. Cell Lines Jurkat (human T lymphocytes, clone E6-1) were obtained from ATCC and cultured in RPMI-1640 (GIBCO) supplemented with 10% heat-inactivated fetal bovine serum (v/v) (GeminiBio), 1X Pen/Strep/Glut, 1mM HEPES, and 100M Sodium Pyruvate. Cells were cultured in a humidified CO2 incubator at 37C. The Jurkat cell line was established from peripheral blood of a 14-year-old male diagnosed with T cell leukemia. Murine primary cell cultures Peritoneal cells were isolated from naïve mice by lavage with 10mL of PBS containing 0.5mM EDTA. Lavage was estimated to contain 50% macrophages. Peritoneal macrophages were isolated by adhesion purification of lavage cells in RPMI-10 (RPMI-1640 containing 10% heat-inactivated fetal bovine serum (v/v), 1X Pen/Strep/Glut, 1mM HEPES, and 100M Sodium Pyruvate) plated 1.3x106 macrophages/well in 6-well plates for FACS sorting or 4x106 macrophage/well in 24-well plates for flow cytometry. Non-adherent cells were removed by washing after 3-5 hours, and macrophages were cultured in fresh RPMI-10 overnight. 400ng/mL purified E. coli O111:B4 LPS (List Biological Laboratories Inc.) was added to generate inflammatory macrophages 12h before the addition of target cells. Bone marrow-derived macrophages were generated by 8-day culture of murine bone marrow cells grown in media containing M-CSF (High glucose DMEM containing 10% v/v FBS, 20% v/v L929-conditioned media, 1X Pen/Strep/Glut, 100M Sodium Pyruvate, 55M beta-Mercaptoethanol). All macrophages were removed from culture dishes by 3-minute incubation with 1X Trypsin-EDTA (Sigma) plus gentle scraping.","SAMPLE_TYPE":"Macrophages"},

"TREATMENT":{"TREATMENT_SUMMARY":"LPS-primed murine peritoneal macrophages were fed fluorescently-tagged IgG-opsonized live Jurkat cells for 1h. Unengulfed targets were washed off and macrophages were left to degrade targets for a further 3 or 7 hours. Macrophages were then collected and sorted into non-engulfing or engulfing populations, alongside control unexposed macrophages. Sorted cells were pelleted at 400xg and dry pellets were snap frozen and stored at -80C until metabolite extraction."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"To process cells for assessment of intracellular metabolites, cells were pelleted at 400xg in tubes coated with 0.06% BSA. Supernatant was aspirated and discarded; residual liquid was carefully wicked away from the pellet with a kimwipe. Dry pellets were immediately snap frozen and stored at -80C until processing. To process culture supernatants for assessment of metabolites, supernatant was centrifuged at 400xg to pellet any cells. Cell-free supernatant was then transferred to a fresh tube, snap frozen, and stored at -80C until processing."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Isocratic flow of 95% phase A (0.1% formic acid in water) and 5% phase B (0.1% formic acid in acetonitrile) at 250 uL/min.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Phenomenex Kinetex C18 (150 x 2.1mm, 2.6 um)","FLOW_GRADIENT":"isocratic","FLOW_RATE":"250 ul/min","COLUMN_TEMPERATURE":"25"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Global metabolomics analyses were performed using a 3 min isocratic run in positive and negative ion modes (separate runs) as described previously (Nemkov et al., 2015, Nemkov et al., 2017); stable isotope tracing samples were analyzed using a 5 min C18 gradient in positive and negative ion modes (separate runs) as described (Nemkov et al., 2019, Gehrke et al., 2019). For all analyses, the MS scanned in MS1 mode across the m/z range of 65 to 950. Peaks were annotated (in conjunction with the KEGG database), integrated, and quality control performed using Maven (Princeton University) as described. Stable isotope tracing results were isotopically corrected for the natural abundance of 13C1, 13C2, 15N1, and 15N2 (Nemkov et al., 2017)."},

"MS_METABOLITE_DATA":{
"Units":"peak area",

"Data":[{"Metabolite":"L-arginine","4h 1":"1.15E+04","4h 2":"3.79E+04","4h 3":"2.33E+04","4h 1-":"2.04E+04","4h 2-":"3.04E+04","4h 3-":"3.68E+04","4h 1+":"5.43E+05","4h 2+":"4.23E+05","4h 3+":"4.42E+05","8h 1":"2.69E+04","8h 2":"3.87E+04","8h 3":"3.32E+04","8h 1-":"3.23E+04","8h 2-":"2.10E+04","8h 3-":"3.61E+04","8h 1+":"2.61E+05","8h 2+":"2.31E+05","8h 3+":"2.70E+05"},{"Metabolite":"L-cysteine","4h 1":"6.20E+04","4h 2":"4.66E+04","4h 3":"4.19E+04","4h 1-":"3.45E+04","4h 2-":"3.45E+04","4h 3-":"6.42E+04","4h 1+":"1.59E+05","4h 2+":"1.94E+05","4h 3+":"2.40E+05","8h 1":"4.36E+04","8h 2":"8.09E+04","8h 3":"4.90E+04","8h 1-":"4.81E+04","8h 2-":"7.27E+04","8h 3-":"4.35E+04","8h 1+":"1.88E+05","8h 2+":"1.53E+05","8h 3+":"1.52E+05"},{"Metabolite":"L-glutamate","4h 1":"3.69E+05","4h 2":"4.36E+05","4h 3":"2.59E+05","4h 1-":"2.93E+05","4h 2-":"2.98E+05","4h 3-":"5.50E+05","4h 1+":"6.12E+05","4h 2+":"4.85E+05","4h 3+":"5.19E+05","8h 1":"4.14E+05","8h 2":"5.77E+05","8h 3":"4.06E+05","8h 1-":"4.08E+05","8h 2-":"5.36E+05","8h 3-":"4.85E+05","8h 1+":"7.93E+05","8h 2+":"6.81E+05","8h 3+":"5.74E+05"},{"Metabolite":"L-glutamine","4h 1":"1.60E+05","4h 2":"1.85E+05","4h 3":"1.28E+05","4h 1-":"1.03E+05","4h 2-":"1.26E+05","4h 3-":"1.93E+05","4h 1+":"4.35E+05","4h 2+":"3.99E+05","4h 3+":"4.83E+05","8h 1":"1.18E+05","8h 2":"1.58E+05","8h 3":"1.42E+05","8h 1-":"1.51E+05","8h 2-":"1.21E+05","8h 3-":"1.87E+05","8h 1+":"5.47E+05","8h 2+":"4.11E+05","8h 3+":"4.61E+05"},{"Metabolite":"glycine","4h 1":"1.41E+06","4h 2":"1.24E+06","4h 3":"1.42E+06","4h 1-":"1.35E+06","4h 2-":"1.29E+06","4h 3-":"1.35E+06","4h 1+":"1.59E+06","4h 2+":"1.29E+06","4h 3+":"1.28E+06","8h 1":"1.41E+06","8h 2":"1.29E+06","8h 3":"1.37E+06","8h 1-":"1.30E+06","8h 2-":"1.36E+06","8h 3-":"1.17E+06","8h 1+":"1.31E+06","8h 2+":"1.46E+06","8h 3+":"1.50E+06"},{"Metabolite":"L-leucine","4h 1":"5.39E+05","4h 2":"1.72E+06","4h 3":"5.41E+05","4h 1-":"4.61E+05","4h 2-":"5.93E+05","4h 3-":"8.80E+05","4h 1+":"2.25E+06","4h 2+":"2.44E+06","4h 3+":"3.34E+06","8h 1":"5.86E+05","8h 2":"5.93E+05","8h 3":"5.44E+05","8h 1-":"6.40E+05","8h 2-":"5.37E+05","8h 3-":"7.73E+05","8h 1+":"1.99E+06","8h 2+":"2.02E+06","8h 3+":"2.42E+06"},{"Metabolite":"L-lysine","4h 1":"4.36E+04","4h 2":"6.37E+04","4h 3":"5.81E+04","4h 1-":"5.96E+04","4h 2-":"4.62E+04","4h 3-":"6.87E+04","4h 1+":"1.28E+06","4h 2+":"8.60E+05","4h 3+":"1.06E+06","8h 1":"4.56E+04","8h 2":"7.18E+04","8h 3":"4.37E+04","8h 1-":"6.90E+04","8h 2-":"6.33E+04","8h 3-":"5.63E+04","8h 1+":"6.91E+05","8h 2+":"5.79E+05","8h 3+":"7.75E+05"},{"Metabolite":"L-methionine","4h 1":"1.22E+04","4h 2":"1.34E+05","4h 3":"2.05E+04","4h 1-":"1.41E+04","4h 2-":"2.23E+04","4h 3-":"2.60E+04","4h 1+":"1.44E+05","4h 2+":"1.21E+05","4h 3+":"1.65E+05","8h 1":"2.01E+04","8h 2":"1.93E+04","8h 3":"1.63E+04","8h 1-":"2.68E+04","8h 2-":"1.43E+04","8h 3-":"2.54E+04","8h 1+":"1.43E+05","8h 2+":"1.02E+05","8h 3+":"1.52E+05"},{"Metabolite":"L-phenylalanine","4h 1":"8.89E+04","4h 2":"2.98E+05","4h 3":"8.77E+04","4h 1-":"9.70E+04","4h 2-":"1.16E+05","4h 3-":"1.69E+05","4h 1+":"2.34E+05","4h 2+":"2.63E+05","4h 3+":"3.42E+05","8h 1":"1.14E+05","8h 2":"1.23E+05","8h 3":"1.14E+05","8h 1-":"1.16E+05","8h 2-":"8.65E+04","8h 3-":"1.29E+05","8h 1+":"3.13E+05","8h 2+":"2.47E+05","8h 3+":"3.00E+05"},{"Metabolite":"L-proline","4h 1":"6.42E+05","4h 2":"8.51E+05","4h 3":"4.67E+05","4h 1-":"5.09E+05","4h 2-":"5.85E+05","4h 3-":"8.45E+05","4h 1+":"1.34E+06","4h 2+":"1.19E+06","4h 3+":"1.67E+06","8h 1":"5.57E+05","8h 2":"6.61E+05","8h 3":"4.62E+05","8h 1-":"7.20E+05","8h 2-":"6.75E+05","8h 3-":"7.05E+05","8h 1+":"1.73E+06","8h 2+":"1.52E+06","8h 3+":"1.42E+06"},{"Metabolite":"L-threonine","4h 1":"4.58E+04","4h 2":"3.77E+04","4h 3":"2.80E+04","4h 1-":"1.49E+04","4h 2-":"3.71E+04","4h 3-":"5.18E+04","4h 1+":"1.03E+05","4h 2+":"8.97E+04","4h 3+":"1.32E+05","8h 1":"2.74E+04","8h 2":"4.84E+04","8h 3":"2.37E+04","8h 1-":"3.36E+04","8h 2-":"3.93E+04","8h 3-":"5.17E+04","8h 1+":"1.32E+05","8h 2+":"1.03E+05","8h 3+":"1.16E+05"},{"Metabolite":"L-tryptophan","4h 1":"3.03E+05","4h 2":"4.17E+05","4h 3":"2.74E+05","4h 1-":"2.30E+05","4h 2-":"2.72E+05","4h 3-":"4.99E+05","4h 1+":"1.15E+06","4h 2+":"1.32E+06","4h 3+":"1.50E+06","8h 1":"3.64E+05","8h 2":"5.22E+05","8h 3":"3.43E+05","8h 1-":"3.60E+05","8h 2-":"2.62E+05","8h 3-":"4.40E+05","8h 1+":"1.48E+06","8h 2+":"1.39E+06","8h 3+":"1.65E+06"},{"Metabolite":"L-tyrosine","4h 1":"7.59E+04","4h 2":"2.58E+05","4h 3":"5.67E+04","4h 1-":"5.99E+04","4h 2-":"7.10E+04","4h 3-":"1.11E+05","4h 1+":"1.93E+05","4h 2+":"1.95E+05","4h 3+":"2.80E+05","8h 1":"7.30E+04","8h 2":"8.11E+04","8h 3":"7.30E+04","8h 1-":"8.18E+04","8h 2-":"5.09E+04","8h 3-":"1.12E+05","8h 1+":"2.53E+05","8h 2+":"2.16E+05","8h 3+":"2.76E+05"},{"Metabolite":"L-valine","4h 1":"9.92E+05","4h 2":"9.33E+05","4h 3":"8.32E+05","4h 1-":"7.77E+05","4h 2-":"8.03E+05","4h 3-":"1.22E+06","4h 1+":"1.00E+06","4h 2+":"9.99E+05","4h 3+":"1.38E+06","8h 1":"8.61E+05","8h 2":"9.98E+05","8h 3":"7.69E+05","8h 1-":"7.80E+05","8h 2-":"8.07E+05","8h 3-":"1.01E+06","8h 1+":"1.13E+06","8h 2+":"7.98E+05","8h 3+":"1.06E+06"},{"Metabolite":"Adenosine","4h 1":"1.23E+06","4h 2":"6.37E+06","4h 3":"1.24E+06","4h 1-":"9.14E+05","4h 2-":"1.19E+06","4h 3-":"2.33E+06","4h 1+":"7.25E+05","4h 2+":"6.02E+05","4h 3+":"1.29E+06","8h 1":"1.91E+06","8h 2":"8.46E+05","8h 3":"1.35E+06","8h 1-":"1.99E+06","8h 2-":"1.01E+06","8h 3-":"2.42E+06","8h 1+":"8.44E+05","8h 2+":"1.64E+06","8h 3+":"1.36E+06"},{"Metabolite":"Adenine","4h 1":"3.33E+04","4h 2":"2.48E+04","4h 3":"3.08E+04","4h 1-":"3.28E+04","4h 2-":"3.32E+04","4h 3-":"4.43E+04","4h 1+":"1.94E+04","4h 2+":"3.28E+04","4h 3+":"2.59E+04","8h 1":"2.94E+04","8h 2":"2.77E+04","8h 3":"5.08E+04","8h 1-":"2.81E+04","8h 2-":"3.17E+04","8h 3-":"3.05E+04","8h 1+":"2.08E+04","8h 2+":"2.90E+04","8h 3+":"4.07E+05"},{"Metabolite":"Guanosine","4h 1":"7.25E+03","4h 2":"7.01E+04","4h 3":"5.60E+03","4h 1-":"0.00E+00","4h 2-":"2.88E+03","4h 3-":"2.16E+04","4h 1+":"1.40E+04","4h 2+":"9.41E+03","4h 3+":"1.85E+04","8h 1":"1.12E+04","8h 2":"2.94E+03","8h 3":"4.15E+03","8h 1-":"1.19E+04","8h 2-":"2.00E+03","8h 3-":"8.73E+03","8h 1+":"2.02E+04","8h 2+":"1.26E+04","8h 3+":"2.72E+04"},{"Metabolite":"Guanine","4h 1":"1.90E+03","4h 2":"2.02E+04","4h 3":"2.31E+03","4h 1-":"2.16E+03","4h 2-":"1.89E+03","4h 3-":"3.85E+03","4h 1+":"1.09E+05","4h 2+":"1.05E+05","4h 3+":"1.11E+05","8h 1":"2.42E+03","8h 2":"2.04E+03","8h 3":"2.38E+03","8h 1-":"4.37E+03","8h 2-":"0.00E+00","8h 3-":"2.55E+03","8h 1+":"3.99E+04","8h 2+":"3.51E+04","8h 3+":"5.45E+04"},{"Metabolite":"Cytosine","4h 1":"5.19E+03","4h 2":"9.69E+03","4h 3":"2.38E+03","4h 1-":"1.41E+04","4h 2-":"7.55E+03","4h 3-":"1.95E+04","4h 1+":"4.61E+05","4h 2+":"2.97E+05","4h 3+":"5.78E+05","8h 1":"7.00E+03","8h 2":"9.83E+03","8h 3":"7.81E+03","8h 1-":"1.32E+04","8h 2-":"1.72E+04","8h 3-":"1.25E+04","8h 1+":"4.01E+05","8h 2+":"2.31E+05","8h 3+":"4.01E+05"},{"Metabolite":"Thymidine","4h 1":"5.98E+03","4h 2":"5.01E+04","4h 3":"1.07E+04","4h 1-":"0.00E+00","4h 2-":"4.17E+04","4h 3-":"3.76E+04","4h 1+":"2.36E+04","4h 2+":"7.93E+03","4h 3+":"8.99E+03","8h 1":"3.68E+03","8h 2":"1.37E+05","8h 3":"3.45E+03","8h 1-":"2.14E+04","8h 2-":"5.73E+03","8h 3-":"7.91E+03","8h 1+":"1.46E+04","8h 2+":"2.34E+05","8h 3+":"1.20E+04"},{"Metabolite":"Thymine","4h 1":"3.05E+04","4h 2":"3.05E+04","4h 3":"2.33E+04","4h 1-":"2.66E+04","4h 2-":"2.92E+04","4h 3-":"3.28E+04","4h 1+":"1.08E+05","4h 2+":"9.65E+04","4h 3+":"1.35E+05","8h 1":"2.72E+04","8h 2":"2.47E+04","8h 3":"2.69E+04","8h 1-":"2.74E+04","8h 2-":"3.46E+04","8h 3-":"9.29E+04","8h 1+":"6.59E+04","8h 2+":"7.24E+04","8h 3+":"7.25E+04"},{"Metabolite":"Uracil","4h 1":"1.68E+05","4h 2":"1.97E+05","4h 3":"8.41E+04","4h 1-":"8.21E+04","4h 2-":"1.30E+05","4h 3-":"1.56E+05","4h 1+":"1.67E+05","4h 2+":"1.23E+05","4h 3+":"1.28E+05","8h 1":"9.75E+04","8h 2":"1.13E+05","8h 3":"6.18E+04","8h 1-":"1.93E+05","8h 2-":"1.11E+05","8h 3-":"5.76E+04","8h 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"Metabolites":[{"Metabolite":"L-arginine","KEGG ID":"C00062","m/z":"175.1190","r.t.":"0.64"},{"Metabolite":"L-cysteine","KEGG ID":"C00097","m/z":"122.0272","r.t.":"0.68"},{"Metabolite":"L-glutamate","KEGG ID":"C00025","m/z":"148.0604","r.t.":"0.67"},{"Metabolite":"L-glutamine","KEGG ID":"C00064","m/z":"147.0764","r.t.":"0.67"},{"Metabolite":"glycine","KEGG ID":"C00037","m/z":"76.0402","r.t.":"1.87"},{"Metabolite":"L-leucine","KEGG ID":"C00123","m/z":"132.1020","r.t.":"0.99"},{"Metabolite":"L-lysine","KEGG ID":"C00047","m/z":"147.1128","r.t.":"0.61"},{"Metabolite":"L-methionine","KEGG ID":"C00073","m/z":"150.0584","r.t.":"0.89"},{"Metabolite":"L-phenylalanine","KEGG ID":"C00079","m/z":"166.0864","r.t.":"1.39"},{"Metabolite":"L-proline","KEGG ID":"C00148","m/z":"116.0710","r.t.":"0.68"},{"Metabolite":"L-threonine","KEGG ID":"C00188","m/z":"120.0658","r.t.":"0.67"},{"Metabolite":"L-tryptophan","KEGG ID":"C00078","m/z":"205.0972","r.t.":"1.72"},{"Metabolite":"L-tyrosine","KEGG ID":"C00082","m/z":"182.0813","r.t.":"0.92"},{"Metabolite":"L-valine","KEGG ID":"C00183","m/z":"118.0866","r.t.":"0.68"},{"Metabolite":"Adenosine","KEGG ID":"C00212","m/z":"268.1039","r.t.":"0.91"},{"Metabolite":"Adenine","KEGG ID":"C00147","m/z":"136.0618","r.t.":"0.26"},{"Metabolite":"Guanosine","KEGG ID":"C00387","m/z":"284.0988","r.t.":"0.94"},{"Metabolite":"Guanine","KEGG ID":"C00242","m/z":"152.0567","r.t.":"0.97"},{"Metabolite":"Cytosine","KEGG ID":"C00380","m/z":"112.0509","r.t.":"0.69"},{"Metabolite":"Thymidine","KEGG ID":"C00214","m/z":"243.0991","r.t.":"1.74"},{"Metabolite":"Thymine","KEGG ID":"C00178","m/z":"127.0504","r.t.":"1.27"},{"Metabolite":"Uracil","KEGG ID":"C00106","m/z":"113.0361","r.t.":"0.59"},{"Metabolite":"Inosine","KEGG ID":"C00294","m/z":"269.0879","r.t.":"0.95"},{"Metabolite":"Hypoxanthine","KEGG ID":"C00262","m/z":"137.0459","r.t.":"1.05"},{"Metabolite":"Xanthine","KEGG ID":"C00385","m/z":"153.0407","r.t.":"0.89"},{"Metabolite":"3',5'-Cyclic IMP","KEGG ID":"C00943","m/z":"331.0447","r.t.":"0.97"},{"Metabolite":"Nicotinamide","KEGG ID":"C00153","m/z":"123.0555","r.t.":"0.91"},{"Metabolite":"Glutathione disulfide","KEGG ID":"C00127","m/z":"613.1587","r.t.":"0.89"},{"Metabolite":"5-Oxoproline","KEGG ID":"C01879","m/z":"130.0500","r.t.":"0.69"},{"Metabolite":"Phosphoserine","KEGG ID":"C02532","m/z":"186.0186","r.t.":"0.59"},{"Metabolite":"3-Phosphonooxypyruvate","KEGG ID":"C03232","m/z":"184.9855","r.t.":"3.79"},{"Metabolite":"Ornithine","KEGG ID":"C01602","m/z":"133.0972","r.t.":"0.61"},{"Metabolite":"L-Citrulline","KEGG ID":"C00327","m/z":"176.1029","r.t.":"0.67"},{"Metabolite":"Putrescine","KEGG ID":"C00134","m/z":"89.1080","r.t.":"0.61"},{"Metabolite":"Spermidine","KEGG ID":"C00315","m/z":"146.1652","r.t.":"0.61"},{"Metabolite":"Spermine","KEGG ID":"C00750","m/z":"203.2230","r.t.":"0.60"},{"Metabolite":"D-Glucosamine","KEGG ID":"C00329","m/z":"180.0866","r.t.":"0.90"},{"Metabolite":"Creatine","KEGG ID":"C00300","m/z":"132.0769","r.t.":"0.67"},{"Metabolite":"4-Acetamidobutanoate","KEGG ID":"C02946","m/z":"146.0812","r.t.":"1.08"},{"Metabolite":"trans-4-Hydroxy-L-proline","KEGG ID":"C01157","m/z":"132.0656","r.t.":"0.71"},{"Metabolite":"Pantothenol","KEGG ID":"C00864","m/z":"220.1180","r.t.":"1.65"},{"Metabolite":"L-Methionine S-oxide","KEGG ID":"C02989","m/z":"166.0533","r.t.":"0.88"},{"Metabolite":"Picolinic acid","KEGG ID":"C10164","m/z":"124.0395","r.t.":"0.23"},{"Metabolite":"Sphingosine","KEGG ID":"C00319","m/z":"300.2896","r.t.":"2.74"},{"Metabolite":"Acetylcholine","KEGG ID":"C01996","m/z":"146.1175","r.t.":"0.72"},{"Metabolite":"Choline","KEGG ID":"C00114","m/z":"104.1075","r.t.":"0.66"},{"Metabolite":"Triacanthine","KEGG ID":"C08435","m/z":"204.1231","r.t.":"0.89"},{"Metabolite":"6-Lactoyl-5-6-7-8-tetrahydropterin","KEGG ID":"C04244","m/z":"240.1090","r.t.":"0.89"},{"Metabolite":"Fe(III)dicitrate","KEGG ID":"C06229","m/z":"245.9450","r.t.":"0.86"},{"Metabolite":"Pyridoxamine","KEGG ID":"C00534","m/z":"169.0961","r.t.":"0.60"},{"Metabolite":"L-Noradrenaline","KEGG ID":"C00547","m/z":"170.0811","r.t.":"0.95"}]
}

}