{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002023","ANALYSIS_ID":"AN003293","VERSION":"1","CREATED_ON":"December 21, 2021, 12:10 am"},

"PROJECT":{"PROJECT_TITLE":"A targeted metabolomics study for assessing rodent thyroid toxicity","PROJECT_SUMMARY":"The thyroid gland regulates various physiological mechanisms in mammals/humans, such as individual development, cell proliferation and differentiation. Thus, disorders can lead to diseases. In this study, we aimed to apply targeted metabolomics approach to investigate rodent thyroid toxicity. For this purpose, male Wistar rats have been exposed to a direct (6-propyl-2-thiouracil, PTU) and an indirect (phenytoin) thyroid toxicant, respectively. Thereby, two doses (low:5ppm for PTU and 300ppm for phenytoin , high: 50ppm for PTU and 2400ppm for phenytoin) and three exposure time phase (short: 2 weeks, long:4 weeks, and long+recovery: 4weeks+2weeks) were investigated, allowing insights into the modes of action during thyroid toxicity. Targeted metabolomics were applied to both liver and thyroid gland tissue.","INSTITUTE":"Helmholtz Centre for Environmental Research GmbH","DEPARTMENT":"Department of Molecular Systems biology","LABORATORY":"Functional Metabolomics","LAST_NAME":"Wang","FIRST_NAME":"Zhipeng","ADDRESS":"Permoserstraße 15, Leipzig, Saxony, 04318, Germany","EMAIL":"zhipeng.wang@ufz.de","PHONE":"+49 341 235 1078"},

"STUDY":{"STUDY_TITLE":"A targeted metabolomics study for assessing rodent thyroid toxicity","STUDY_SUMMARY":"The thyroid gland regulates various physiological mechanisms in mammals/humans, such as individual development, cell proliferation and differentiation. Thus, disorders can lead to diseases. In this study, we aimed to apply targeted metabolomics approach to investigate rodent thyroid toxicity. For this purpose, male Wistar rats have been exposed to a direct (6-propyl-2-thiouracil, PTU) and an indirect (phenytoin) thyroid toxicant, respectively. Thereby, two doses (low:5ppm for PTU and 300ppm for phenytoin , high: 50ppm for PTU and 2400ppm for phenytoin) and three exposure time phase (short: 2 weeks, long:4 weeks, and long+recovery: 4weeks+2weeks) were investigated, allowing insights into the modes of action during thyroid toxicity. Targeted metabolomics were applied to both liver and thyroid gland tissue.","INSTITUTE":"Helmholtz Centre for Environmental Research GmbH","DEPARTMENT":"Department of Molecular Systems biology","LABORATORY":"Functional Metabolomics","LAST_NAME":"Wang","FIRST_NAME":"Zhipeng","ADDRESS":"Permoserstraße 15, Leipzig, Saxony, 04318, Germany","EMAIL":"zhipeng.wang@ufz.de","PHONE":"+49 341 235 1078"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Rattus norvegicus","TAXONOMY_ID":"10116"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"L-A2-G1-M",
"Factors":{"Short sample name":"L2","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A4-G1-M",
"Factors":{"Short sample name":"L4","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A5-G1-M",
"Factors":{"Short sample name":"L5","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A6-G1-M",
"Factors":{"Short sample name":"L6","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A8-G1-M",
"Factors":{"Short sample name":"L8","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A12-G2-M",
"Factors":{"Short sample name":"L12","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A15-G2-M",
"Factors":{"Short sample name":"L15","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A16-G2-M",
"Factors":{"Short sample name":"L16","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A17-G2-M",
"Factors":{"Short sample name":"L17","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A18-G2-M",
"Factors":{"Short sample name":"L18","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A22-G3-M",
"Factors":{"Short sample name":"L22","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A23-G3-M",
"Factors":{"Short sample name":"L23","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A25-G3-M",
"Factors":{"Short sample name":"L25","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A28-G3-M",
"Factors":{"Short sample name":"L28","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A30-G3-M",
"Factors":{"Short sample name":"L30","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A32-G4-M",
"Factors":{"Short sample name":"L32","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A33-G4-M",
"Factors":{"Short sample name":"L33","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A34-G4-M",
"Factors":{"Short sample name":"L34","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A36-G4-M",
"Factors":{"Short sample name":"L36","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A38-G4-M",
"Factors":{"Short sample name":"L38","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A43-G5-M",
"Factors":{"Short sample name":"L43","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A45-G5-M",
"Factors":{"Short sample name":"L45","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A47-G5-M",
"Factors":{"Short sample name":"L47","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A48-G5-M",
"Factors":{"Short sample name":"L48","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A49-G5-M",
"Factors":{"Short sample name":"L49","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A52-G6-M",
"Factors":{"Short sample name":"L52","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A53-G6-M",
"Factors":{"Short sample name":"L53","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A55-G6-M",
"Factors":{"Short sample name":"L55","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A56-G6-M",
"Factors":{"Short sample name":"L56","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A58-G6-M",
"Factors":{"Short sample name":"L58","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A62-G7-M",
"Factors":{"Short sample name":"L62","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A65-G7-M",
"Factors":{"Short sample name":"L65","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A66-G7-M",
"Factors":{"Short sample name":"L66","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A67-G7-M",
"Factors":{"Short sample name":"L67","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A70-G7-M",
"Factors":{"Short sample name":"L70","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A72-G8-M",
"Factors":{"Short sample name":"L72","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A73-G8-M",
"Factors":{"Short sample name":"L73","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A74-G8-M",
"Factors":{"Short sample name":"L74","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A76-G8-M",
"Factors":{"Short sample name":"L76","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A80-G8-M",
"Factors":{"Short sample name":"L80","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A82-G9-M",
"Factors":{"Short sample name":"L82","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A85-G9-M",
"Factors":{"Short sample name":"L85","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A88-G9-M",
"Factors":{"Short sample name":"L88","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A89-G9-M",
"Factors":{"Short sample name":"L89","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A90-G9-M",
"Factors":{"Short sample name":"L90","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A92-G10-M",
"Factors":{"Short sample name":"L92","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A93-G10-M",
"Factors":{"Short sample name":"L93","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A94-G10-M",
"Factors":{"Short sample name":"L94","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A96-G10-M",
"Factors":{"Short sample name":"L96","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A98-G10-M",
"Factors":{"Short sample name":"L98","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A103-G11-M",
"Factors":{"Short sample name":"L103","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A104-G11-M",
"Factors":{"Short sample name":"L104","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A105-G11-M",
"Factors":{"Short sample name":"L105","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A108-G11-M",
"Factors":{"Short sample name":"L108","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A110-G11-M",
"Factors":{"Short sample name":"L110","Organ":"Liver","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A112-G12-M",
"Factors":{"Short sample name":"L112","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A114-G12-M",
"Factors":{"Short sample name":"L114","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A116-G12-M",
"Factors":{"Short sample name":"L116","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A117-G12-M",
"Factors":{"Short sample name":"L117","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A118-G12-M",
"Factors":{"Short sample name":"L118","Organ":"Liver","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A122-G13-M",
"Factors":{"Short sample name":"L122","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A123-G13-M",
"Factors":{"Short sample name":"L123","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A124-G13-M",
"Factors":{"Short sample name":"L124","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A127-G13-M",
"Factors":{"Short sample name":"L127","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A128-G13-M",
"Factors":{"Short sample name":"L128","Organ":"Liver","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A132-G14-M",
"Factors":{"Short sample name":"L132","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A135-G14-M",
"Factors":{"Short sample name":"L135","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A136-G14-M",
"Factors":{"Short sample name":"L136","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A139-G14-M",
"Factors":{"Short sample name":"L139","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A140-G14-M",
"Factors":{"Short sample name":"L140","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A142-G15-M",
"Factors":{"Short sample name":"L142","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A143-G15-M",
"Factors":{"Short sample name":"L143","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A144-G15-M",
"Factors":{"Short sample name":"L144","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A145-G15-M",
"Factors":{"Short sample name":"L145","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"L-A148-G15-M",
"Factors":{"Short sample name":"L148","Organ":"Liver","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A2-G1-M",
"Factors":{"Short sample name":"S2","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A4-G1-M",
"Factors":{"Short sample name":"S4","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A5-G1-M",
"Factors":{"Short sample name":"S5","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A6-G1-M",
"Factors":{"Short sample name":"S6","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A8-G1-M",
"Factors":{"Short sample name":"S8","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A12-G2-M",
"Factors":{"Short sample name":"S12","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A15-G2-M",
"Factors":{"Short sample name":"S15","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A16-G2-M",
"Factors":{"Short sample name":"S16","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A17-G2-M",
"Factors":{"Short sample name":"S17","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A18-G2-M",
"Factors":{"Short sample name":"S18","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A22-G3-M",
"Factors":{"Short sample name":"S22","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A23-G3-M",
"Factors":{"Short sample name":"S23","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A25-G3-M",
"Factors":{"Short sample name":"S25","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A28-G3-M",
"Factors":{"Short sample name":"S28","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A30-G3-M",
"Factors":{"Short sample name":"S30","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A32-G4-M",
"Factors":{"Short sample name":"S32","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A33-G4-M",
"Factors":{"Short sample name":"S33","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A34-G4-M",
"Factors":{"Short sample name":"S34","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A36-G4-M",
"Factors":{"Short sample name":"S36","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A38-G4-M",
"Factors":{"Short sample name":"S38","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A43-G5-M",
"Factors":{"Short sample name":"S43","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A45-G5-M",
"Factors":{"Short sample name":"S45","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A47-G5-M",
"Factors":{"Short sample name":"S47","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A48-G5-M",
"Factors":{"Short sample name":"S48","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A49-G5-M",
"Factors":{"Short sample name":"S49","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"2 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A52-G6-M",
"Factors":{"Short sample name":"S52","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A53-G6-M",
"Factors":{"Short sample name":"S53","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A55-G6-M",
"Factors":{"Short sample name":"S55","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A56-G6-M",
"Factors":{"Short sample name":"S56","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A58-G6-M",
"Factors":{"Short sample name":"S58","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A62-G7-M",
"Factors":{"Short sample name":"S62","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A65-G7-M",
"Factors":{"Short sample name":"S65","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A66-G7-M",
"Factors":{"Short sample name":"S66","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A70-G7-M",
"Factors":{"Short sample name":"S70","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A72-G8-M",
"Factors":{"Short sample name":"S72","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A73-G8-M",
"Factors":{"Short sample name":"S73","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A74-G8-M",
"Factors":{"Short sample name":"S74","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A76-G8-M",
"Factors":{"Short sample name":"S76","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A80-G8-M",
"Factors":{"Short sample name":"S80","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A82-G9-M",
"Factors":{"Short sample name":"S82","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A85-G9-M",
"Factors":{"Short sample name":"S85","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A88-G9-M",
"Factors":{"Short sample name":"S88","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A89-G9-M",
"Factors":{"Short sample name":"S89","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A90-G9-M",
"Factors":{"Short sample name":"S90","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A92-G10-M",
"Factors":{"Short sample name":"S92","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A93-G10-M",
"Factors":{"Short sample name":"S93","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A94-G10-M",
"Factors":{"Short sample name":"S94","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A96-G10-M",
"Factors":{"Short sample name":"S96","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A98-G10-M",
"Factors":{"Short sample name":"S98","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"0 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A103-G11-M",
"Factors":{"Short sample name":"S103","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A104-G11-M",
"Factors":{"Short sample name":"S104","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A105-G11-M",
"Factors":{"Short sample name":"S105","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A108-G11-M",
"Factors":{"Short sample name":"S108","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A110-G11-M",
"Factors":{"Short sample name":"S110","Organ":"Thyroid","Treatment":"Control","Concentration":"-","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A112-G12-M",
"Factors":{"Short sample name":"S112","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A114-G12-M",
"Factors":{"Short sample name":"S114","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A116-G12-M",
"Factors":{"Short sample name":"S116","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A117-G12-M",
"Factors":{"Short sample name":"S117","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A118-G12-M",
"Factors":{"Short sample name":"S118","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"300 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A122-G13-M",
"Factors":{"Short sample name":"S122","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A123-G13-M",
"Factors":{"Short sample name":"S123","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A124-G13-M",
"Factors":{"Short sample name":"S124","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A127-G13-M",
"Factors":{"Short sample name":"S127","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A128-G13-M",
"Factors":{"Short sample name":"S128","Organ":"Thyroid","Treatment":"Phenytoin","Concentration":"2400 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A132-G14-M",
"Factors":{"Short sample name":"S132","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A135-G14-M",
"Factors":{"Short sample name":"S135","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A136-G14-M",
"Factors":{"Short sample name":"S136","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A139-G14-M",
"Factors":{"Short sample name":"S139","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A140-G14-M",
"Factors":{"Short sample name":"S140","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"5 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A142-G15-M",
"Factors":{"Short sample name":"S142","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A143-G15-M",
"Factors":{"Short sample name":"S143","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A144-G15-M",
"Factors":{"Short sample name":"S144","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A145-G15-M",
"Factors":{"Short sample name":"S145","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
},
{
"Subject ID":"-",
"Sample ID":"S-A148-G15-M",
"Factors":{"Short sample name":"S148","Organ":"Thyroid","Treatment":"6-propyl-2-thiouracil","Concentration":"50 ppm","Period":"4 weeks","Recovery":"2 weeks"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"The animal experiment was done by our cooperative partner BASF. The liver and thyroid tissue samples were delivered to us with the protection of dry ice. And all samples was stored immediately under -80℃ until further analysis.","SAMPLE_TYPE":"Tissues"},

"TREATMENT":{"TREATMENT_SUMMARY":"Two well-described test chemicals, i.e. Phenytoin and Propylthiouracil, were administered via the diet at each two concentrations to male Wistar rats for 2 and 4 weeks. In addition, a recovery period of 2 weeks without test substance application was included in order to monitor reversibility of the induced effects. Phenytoin was administered concentrations of 300 and 2400 ppm, Propylthiouracil was administered at concentrations of 5 and 50 ppm. Control animals received maintenance diet only without inclusion of any test substance."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Tissue metabolites were extracted according to our laboratory standard protocol. Briefly, about 100 mg liver and all received thyroid gland (2.4 - 34.1 mg) tissue materials were weighed and mixed with 5x extraction buffer (ACN / H2O: 1:1). With the help of added 4 metal balls, the tissue metabolites were separated by using a tissue slicer for 10 min at 30 Hz. After centrifugation of 2 min at 14,000 rpm, the metabolites containing supernatant were transfered to a fresh tube, and stored at -80℃ for further analysis. The targeted metabolomics was done by using MxP® Quant 500 kit (Biocrates). All analysis steps was followed by the manufactures from Biocrates."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Other","INSTRUMENT_NAME":"Agilent 1290 Infinity","COLUMN_NAME":"MxP Quant 500 Column System"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 5500 QTrap","INSTRUMENT_TYPE":"QTRAP","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"Both FIA and LC methods were used. Both positive and negative were used. The MS acquisition and Data processing were done following the manufacturer's instructions. from Biocrates"},

"MS_METABOLITE_DATA":{
"Units":"µM",

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