{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002054","ANALYSIS_ID":"AN003344","VERSION":"1","CREATED_ON":"January 3, 2022, 11:58 am"},
"PROJECT":{"PROJECT_TITLE":"Jorida Data","PROJECT_SUMMARY":"Reduced ER-mitochondria connectivity promotes neuroblastoma multidrug resistance.","INSTITUTE":"Columbia University - Medical Center","DEPARTMENT":"Neurology","LABORATORY":"Area-Gomez Lab","LAST_NAME":"Yun","FIRST_NAME":"Taekyung","ADDRESS":"650 W 168th Street, New York, NY, 10032, USA","EMAIL":"tdy2102@cumc.columbia.edu","PHONE":"212-305-3836"},
"STUDY":{"STUDY_TITLE":"Jorida Data","STUDY_SUMMARY":"Reduced ER-mitochondria connectivity promotes neuroblastoma multidrug resistance.","INSTITUTE":"Columbia University","DEPARTMENT":"Neurology","LABORATORY":"Area-Gomez Lab","LAST_NAME":"Yun","FIRST_NAME":"Taekyung","ADDRESS":"650 W 168th Street","EMAIL":"tdy2102@cumc.columbia.edu","PHONE":"212-305-3836"},
"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 02",
"Factors":{"Genotype":"CHLA 136"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 02","RAW_FILE_NAME":"2021_06_16 Jorida Neg 02"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 03",
"Factors":{"Genotype":"COGN 144"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 03","RAW_FILE_NAME":"2021_06_16 Jorida Neg 03"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 04",
"Factors":{"Genotype":"BE 2"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 04","RAW_FILE_NAME":"2021_06_16 Jorida Neg 04"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 05",
"Factors":{"Genotype":"CHLA 122"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 05","RAW_FILE_NAME":"2021_06_16 Jorida Neg 05"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 06",
"Factors":{"Genotype":"CHLA 15"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 06","RAW_FILE_NAME":"2021_06_16 Jorida Neg 06"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 07",
"Factors":{"Genotype":"BE 2"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 07","RAW_FILE_NAME":"2021_06_16 Jorida Neg 07"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 08",
"Factors":{"Genotype":"CHLA 136"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 08","RAW_FILE_NAME":"2021_06_16 Jorida Neg 08"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 10",
"Factors":{"Genotype":"CHLA 15"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 10","RAW_FILE_NAME":"2021_06_16 Jorida Neg 10"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 11",
"Factors":{"Genotype":"CHLA 20"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 11","RAW_FILE_NAME":"2021_06_16 Jorida Neg 11"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 12",
"Factors":{"Genotype":"CHLA 122"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 12","RAW_FILE_NAME":"2021_06_16 Jorida Neg 12"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 13",
"Factors":{"Genotype":"COGN 144"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 13","RAW_FILE_NAME":"2021_06_16 Jorida Neg 13"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 14",
"Factors":{"Genotype":"COGN 145"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 14","RAW_FILE_NAME":"2021_06_16 Jorida Neg 14"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 15",
"Factors":{"Genotype":"CHLA 136"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 15","RAW_FILE_NAME":"2021_06_16 Jorida Neg 15"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 16",
"Factors":{"Genotype":"BE 1"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 16","RAW_FILE_NAME":"2021_06_16 Jorida Neg 16"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 18",
"Factors":{"Genotype":"CHLA 20"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 18","RAW_FILE_NAME":"2021_06_16 Jorida Neg 18"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 19",
"Factors":{"Genotype":"CHLA 122"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 19","RAW_FILE_NAME":"2021_06_16 Jorida Neg 19"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 20",
"Factors":{"Genotype":"CHLA 15"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 20","RAW_FILE_NAME":"2021_06_16 Jorida Neg 20"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 21",
"Factors":{"Genotype":"CHLA 20"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 21","RAW_FILE_NAME":"2021_06_16 Jorida Neg 21"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 22",
"Factors":{"Genotype":"CHLA 15"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 22","RAW_FILE_NAME":"2021_06_16 Jorida Neg 22"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 23",
"Factors":{"Genotype":"COGN 145"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 23","RAW_FILE_NAME":"2021_06_16 Jorida Neg 23"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 24",
"Factors":{"Genotype":"COGN 145"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 24","RAW_FILE_NAME":"2021_06_16 Jorida Neg 24"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 26",
"Factors":{"Genotype":"BE 1"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 26","RAW_FILE_NAME":"2021_06_16 Jorida Neg 26"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 27",
"Factors":{"Genotype":"BE 1"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 27","RAW_FILE_NAME":"2021_06_16 Jorida Neg 27"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 28",
"Factors":{"Genotype":"COGN 144"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 28","RAW_FILE_NAME":"2021_06_16 Jorida Neg 28"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 29",
"Factors":{"Genotype":"CHLA 122"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 29","RAW_FILE_NAME":"2021_06_16 Jorida Neg 29"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 30",
"Factors":{"Genotype":"COGN 144"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 30","RAW_FILE_NAME":"2021_06_16 Jorida Neg 30"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 31",
"Factors":{"Genotype":"CHLA 136"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 31","RAW_FILE_NAME":"2021_06_16 Jorida Neg 31"}
},
{
"Subject ID":"-",
"Sample ID":"2021_06_16_Jorida_Run 32",
"Factors":{"Genotype":"BE 2"},
"Additional sample data":{"RAW_FILE_NAME":"2021_06_16 Jorida Pos 32","RAW_FILE_NAME":"2021_06_16 Jorida Neg 32"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Cells were collected in cold PBS and suspended in cold Buffer A with protease inhibitor at 10 million cells/ml","SAMPLE_TYPE":"Cultured cells"},
"TREATMENT":{"TREATMENT_SUMMARY":"Cells were treated with IMDM Media 12440 supplemented in 10% fetal bovine serum, 2mM L-glutamine, 1% ITS, 100U/ml of penicillin and 100 mcg/ml gentamycin. Cell cultures were incubated at 37 degree Celsius in a humidified atmosphere of 5% CO2."},
"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Lipids were extracted from the cell pellet (Bligh/Dyer). 900 ul chlorofrom/methanol (1/2) into the cell pellet, then incubated at 4C for 1 hour agigated (300rpm). Chloroform(300ul) and MQ water(250ul) were added discrete order, then vortex and cetrifuged for 2min at 9000 rpm. Bottom organic phase was collected and dried using vacuum concentrator."},
"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity I-Class","COLUMN_NAME":"Waters Acquity BEH HSS T3 (150x 2.1mm, 1.8um)"},
"ANALYSIS":{"ANALYSIS_TYPE":"MS"},
"MS":{"INSTRUMENT_NAME":"Waters Synapt G2 Si QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Raw data were converted into ABF files using Analysis Base File Converter. These converted files were used for MS-Dial to align/identify peaks/calculate peak area."},
"MS_METABOLITE_DATA":{
"Units":"uM",
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