{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002086","ANALYSIS_ID":"AN003404","VERSION":"1","CREATED_ON":"February 16, 2022, 2:03 am"},

"PROJECT":{"PROJECT_TITLE":"Serum NMR profiling reveals differential alterations in the lipoproteome induced by Pfizer-BioNTech vaccine in COVID-19–recovered subjects and naïve subjects","PROJECT_TYPE":"NMR-based metabolomic analysis","PROJECT_SUMMARY":"1H NMR spectra of sera have been used to define the changes induced by vaccination with Pfizer-BioNTech vaccine (2 shots, 21 days apart) in 10 COVID-19-recovered subjects and 10 COVID-19-naïve subjects at different time points, starting from before vaccination, then weekly until 7 days after second injection, and finally 1 month after the second dose. The data show that vaccination does not induce any significant variation in the metabolome, whereas it causes changes at the level of lipoproteins. The effects are different in the COVID-19-recovered subjects with respect to the naïve subjects, suggesting that a previous infection reduces the vaccine modulation of the lipoproteome composition.","INSTITUTE":"University of Florence","DEPARTMENT":"Department of chemistry","LABORATORY":"Metabolomics","LAST_NAME":"Ghini","FIRST_NAME":"Veronica","ADDRESS":"via Luigi Sacconi 6","EMAIL":"ghini@cerm.unifi.it","PHONE":"+390554574266"},

"STUDY":{"STUDY_TITLE":"Serum NMR profiling reveals differential alterations in the lipoproteome induced by Pfizer-BioNTech vaccine in COVID-19–recovered subjects and naïve subjects","STUDY_TYPE":"NMR-based metabolomics","STUDY_SUMMARY":"1H NMR spectra of sera have been used to define the changes induced by vaccination with Pfizer-BioNTech vaccine (2 shots, 21 days apart) in 10 COVID-19-recovered subjects and 10 COVID-19-naïve subjects at different time points, starting from before vaccination, then weekly until 7 days after second injection, and finally 1 month after the second dose. The data show that vaccination does not induce any significant variation in the metabolome, whereas it causes changes at the level of lipoproteins. The effects are different in the COVID-19-recovered subjects with respect to the naïve subjects, suggesting that a previous infection reduces the vaccine modulation of the lipoproteome composition.","INSTITUTE":"University of Florence","LAST_NAME":"Ghini","FIRST_NAME":"Veronica","ADDRESS":"via Luigi Sacconi","EMAIL":"ghini@cerm.unifi.it","PHONE":"03922800462","NUM_GROUPS":"2","TOTAL_SUBJECTS":"20","NUM_MALES":"7","NUM_FEMALES":"13"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"24-65","GENDER":"Male and female","HUMAN_ETHNICITY":"Caucasian"},
"SUBJECT_SAMPLE_FACTORS":[
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"Factors":{"Previus_COVID":"No","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_AF_T0","SEX":"male"}
},
{
"Subject ID":"1",
"Sample ID":"2-Serum_AF_T7",
"Factors":{"Previus_COVID":"No","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_AF_T07","SEX":"male"}
},
{
"Subject ID":"1",
"Sample ID":"3-Serum_AF_T14",
"Factors":{"Previus_COVID":"No","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_AF_T14","SEX":"male"}
},
{
"Subject ID":"1",
"Sample ID":"4-Serum_AF_T21",
"Factors":{"Previus_COVID":"No","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_AF_T21","SEX":"male"}
},
{
"Subject ID":"1",
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"Factors":{"Previus_COVID":"No","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_AF_T28","SEX":"male"}
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{
"Subject ID":"1",
"Sample ID":"6-Serum_AF_T1M",
"Factors":{"Previus_COVID":"No","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_AF_T1M","SEX":"male"}
},
{
"Subject ID":"2",
"Sample ID":"7-Serum_CI_T0",
"Factors":{"Previus_COVID":"No","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CI_T0","SEX":"female"}
},
{
"Subject ID":"2",
"Sample ID":"8-Serum_CI_T07",
"Factors":{"Previus_COVID":"No","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CI_T07","SEX":"female"}
},
{
"Subject ID":"2",
"Sample ID":"9-Serum_CI_T14",
"Factors":{"Previus_COVID":"No","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CI_T14","SEX":"female"}
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{
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"Sample ID":"10-Serum_CI_T21_200ul",
"Factors":{"Previus_COVID":"No","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CI_T21_200ul","SEX":"female"}
},
{
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"Sample ID":"11-Serum_CI_T28",
"Factors":{"Previus_COVID":"No","TIME":"T28"},
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{
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"Sample ID":"12-Serum_CI_T1M",
"Factors":{"Previus_COVID":"No","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CI_T1M","SEX":"female"}
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{
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"Sample ID":"13-Serum_CC_T07_250uL",
"Factors":{"Previus_COVID":"Yes","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CC_T07_250uL","SEX":"female"}
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"Sample ID":"14-Serum_CC_T14_210uL",
"Factors":{"Previus_COVID":"Yes","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CC_T14_210uL","SEX":"female"}
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{
"Subject ID":"3",
"Sample ID":"15-Serum_CC_T21",
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"Sample ID":"16-Serum_CC_T28",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CC_T28","SEX":"female"}
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{
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"Sample ID":"17-Serum_CC_T0_250uL",
"Factors":{"Previus_COVID":"Yes","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CC_T0_250uL","SEX":"female"}
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"Subject ID":"3",
"Sample ID":"18-Serum_CC_T1M",
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"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CC_T1M","SEX":"female"}
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{
"Subject ID":"4",
"Sample ID":"19-Serum_CCa_T07",
"Factors":{"Previus_COVID":"Yes","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CCa_T07","SEX":"female"}
},
{
"Subject ID":"4",
"Sample ID":"20-Serum_CCa_T14",
"Factors":{"Previus_COVID":"Yes","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CCa_T14","SEX":"female"}
},
{
"Subject ID":"4",
"Sample ID":"21-Serum_CCa_T21",
"Factors":{"Previus_COVID":"Yes","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CCa_T21","SEX":"female"}
},
{
"Subject ID":"4",
"Sample ID":"22-Serum_CCa_T28",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CCa_T28","SEX":"female"}
},
{
"Subject ID":"4",
"Sample ID":"23-Serum_CCa_T0_210uL",
"Factors":{"Previus_COVID":"Yes","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CCa_T0_210uL","SEX":"female"}
},
{
"Subject ID":"4",
"Sample ID":"24-Serum_CCa_T1M",
"Factors":{"Previus_COVID":"Yes","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CCa_T1M","SEX":"female"}
},
{
"Subject ID":"5",
"Sample ID":"25-Serum_CS_T0",
"Factors":{"Previus_COVID":"Yes","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CS_T0","SEX":"male"}
},
{
"Subject ID":"5",
"Sample ID":"26-Serum_CS_T7",
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"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CS_T07","SEX":"male"}
},
{
"Subject ID":"5",
"Sample ID":"27-Serum_CS_T14",
"Factors":{"Previus_COVID":"Yes","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CS_T14","SEX":"male"}
},
{
"Subject ID":"5",
"Sample ID":"28-Serum_CS_T21",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CS_T21","SEX":"male"}
},
{
"Subject ID":"5",
"Sample ID":"29-Serum_CS_T28",
"Factors":{"Previus_COVID":"Yes","TIME":"T28"},
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},
{
"Subject ID":"6",
"Sample ID":"30-Serum_CMG_T0",
"Factors":{"Previus_COVID":"No","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CMG_T0","SEX":"female"}
},
{
"Subject ID":"6",
"Sample ID":"31-Serum_CMG_T7",
"Factors":{"Previus_COVID":"No","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CMG_T07","SEX":"female"}
},
{
"Subject ID":"6",
"Sample ID":"32-Serum_CMG_T14",
"Factors":{"Previus_COVID":"No","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CMG_T14","SEX":"female"}
},
{
"Subject ID":"6",
"Sample ID":"33-Serum_CMG_T21",
"Factors":{"Previus_COVID":"No","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CMG_T21","SEX":"female"}
},
{
"Subject ID":"6",
"Sample ID":"34-Serum_CMG_T28",
"Factors":{"Previus_COVID":"No","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CMG_T28","SEX":"female"}
},
{
"Subject ID":"6",
"Sample ID":"35-Serum_CMG_T1M",
"Factors":{"Previus_COVID":"No","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CMG_T1M","SEX":"female"}
},
{
"Subject ID":"7",
"Sample ID":"36-Serum_CG_T0",
"Factors":{"Previus_COVID":"Yes","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CG_T0","SEX":"male"}
},
{
"Subject ID":"7",
"Sample ID":"37-Serum_CG_T7",
"Factors":{"Previus_COVID":"Yes","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CG_T07","SEX":"male"}
},
{
"Subject ID":"7",
"Sample ID":"38-Serum_CG_T14",
"Factors":{"Previus_COVID":"Yes","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CG_T14","SEX":"male"}
},
{
"Subject ID":"7",
"Sample ID":"39-Serum_CG_T21",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CG_T21","SEX":"male"}
},
{
"Subject ID":"7",
"Sample ID":"40-Serum_CG_T28",
"Factors":{"Previus_COVID":"Yes","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CG_T28","SEX":"male"}
},
{
"Subject ID":"7",
"Sample ID":"41-Serum_CG_T1M",
"Factors":{"Previus_COVID":"Yes","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CG_T1M","SEX":"male"}
},
{
"Subject ID":"8",
"Sample ID":"42-Serum_CA_T0",
"Factors":{"Previus_COVID":"Yes","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CA_T0","SEX":"female"}
},
{
"Subject ID":"8",
"Sample ID":"43-Serum_CA_T7",
"Factors":{"Previus_COVID":"Yes","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CA_T07","SEX":"female"}
},
{
"Subject ID":"8",
"Sample ID":"44-Serum_CA_T14",
"Factors":{"Previus_COVID":"Yes","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CA_T14","SEX":"female"}
},
{
"Subject ID":"8",
"Sample ID":"45-Serum_CA_T21",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CA_T21","SEX":"female"}
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{
"Subject ID":"8",
"Sample ID":"46-Serum_CA_T28",
"Factors":{"Previus_COVID":"Yes","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CA_T28","SEX":"female"}
},
{
"Subject ID":"8",
"Sample ID":"47-Serum_CA_T1M",
"Factors":{"Previus_COVID":"Yes","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_CA_T1M","SEX":"female"}
},
{
"Subject ID":"9",
"Sample ID":"48-Serum_DD_T0",
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"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_DD_T0","SEX":"female"}
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{
"Subject ID":"9",
"Sample ID":"49-Serum_DD_T07",
"Factors":{"Previus_COVID":"Yes","TIME":"T7"},
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},
{
"Subject ID":"9",
"Sample ID":"50-Serum_DD_T21",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
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"Subject ID":"9",
"Sample ID":"51-Serum_DD_T28",
"Factors":{"Previus_COVID":"Yes","TIME":"T28"},
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"Factors":{"Previus_COVID":"No","TIME":"T1M"},
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},
{
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"Factors":{"Previus_COVID":"No","TIME":"T0"},
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},
{
"Subject ID":"12",
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"Factors":{"Previus_COVID":"No","TIME":"T7"},
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},
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"Subject ID":"12",
"Sample ID":"67-Serum_MF_T14",
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},
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},
{
"Subject ID":"12",
"Sample ID":"70-Serum_MF_T1M",
"Factors":{"Previus_COVID":"No","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_MF_T1M","SEX":"female"}
},
{
"Subject ID":"13",
"Sample ID":"71-Serum_MC_T0",
"Factors":{"Previus_COVID":"No","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_MC_T0","SEX":"female"}
},
{
"Subject ID":"13",
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"Factors":{"Previus_COVID":"No","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_MC_T07","SEX":"female"}
},
{
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"Sample ID":"73-Serum_MC_T14",
"Factors":{"Previus_COVID":"No","TIME":"T14"},
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},
{
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"Factors":{"Previus_COVID":"No","TIME":"T21"},
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},
{
"Subject ID":"13",
"Sample ID":"75-Serum_MC_T28",
"Factors":{"Previus_COVID":"No","TIME":"T28"},
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},
{
"Subject ID":"13",
"Sample ID":"76-Serum_MC_T1M",
"Factors":{"Previus_COVID":"No","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_MC_T1M","SEX":"female"}
},
{
"Subject ID":"14",
"Sample ID":"77-Serum_NF_T0",
"Factors":{"Previus_COVID":"Yes","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_NF_T0","SEX":"male"}
},
{
"Subject ID":"14",
"Sample ID":"78-Serum_NF_T7",
"Factors":{"Previus_COVID":"Yes","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_NF_T07","SEX":"male"}
},
{
"Subject ID":"14",
"Sample ID":"79-Serum_NF_T14",
"Factors":{"Previus_COVID":"Yes","TIME":"T14"},
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},
{
"Subject ID":"14",
"Sample ID":"80-Serum_NF_T21",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_NF_T21","SEX":"male"}
},
{
"Subject ID":"14",
"Sample ID":"81-Serum_NF_T28",
"Factors":{"Previus_COVID":"Yes","TIME":"T28"},
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},
{
"Subject ID":"14",
"Sample ID":"82-Serum_NF_T1M",
"Factors":{"Previus_COVID":"Yes","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_NF_T1M","SEX":"male"}
},
{
"Subject ID":"15",
"Sample ID":"83-Serum_PF_T0",
"Factors":{"Previus_COVID":"Yes","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PF_T0","SEX":"male"}
},
{
"Subject ID":"15",
"Sample ID":"84-Serum_PF_T7",
"Factors":{"Previus_COVID":"Yes","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PF_T07","SEX":"male"}
},
{
"Subject ID":"15",
"Sample ID":"85-Serum_PF_T14",
"Factors":{"Previus_COVID":"Yes","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PF_T14","SEX":"male"}
},
{
"Subject ID":"15",
"Sample ID":"86-Serum_PF_T21",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PF_T21","SEX":"male"}
},
{
"Subject ID":"15",
"Sample ID":"87-Serum_PF_T1M",
"Factors":{"Previus_COVID":"Yes","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PF_T1M","SEX":"female"}
},
{
"Subject ID":"16",
"Sample ID":"88-Serum_PS_T0",
"Factors":{"Previus_COVID":"No","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PS_T0","SEX":"female"}
},
{
"Subject ID":"16",
"Sample ID":"89-Serum_PS_T7",
"Factors":{"Previus_COVID":"No","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PS_T07","SEX":"female"}
},
{
"Subject ID":"16",
"Sample ID":"90-Serum_PS_T14",
"Factors":{"Previus_COVID":"No","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PS_T14","SEX":"female"}
},
{
"Subject ID":"16",
"Sample ID":"91-Serum_PS_T21",
"Factors":{"Previus_COVID":"No","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PS_T21","SEX":"female"}
},
{
"Subject ID":"16",
"Sample ID":"92-Serum_PS_T28",
"Factors":{"Previus_COVID":"No","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PS_T28","SEX":"female"}
},
{
"Subject ID":"16",
"Sample ID":"93-Serum_PS_T1M",
"Factors":{"Previus_COVID":"No","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_PS_T1M","SEX":"female"}
},
{
"Subject ID":"17",
"Sample ID":"94-Serum_RA_T0",
"Factors":{"Previus_COVID":"No","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_RA_T0","SEX":"female"}
},
{
"Subject ID":"17",
"Sample ID":"95-Serum_RA_T07_250uL",
"Factors":{"Previus_COVID":"No","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_RA_T07_250uL","SEX":"female"}
},
{
"Subject ID":"17",
"Sample ID":"96-Serum_RA_T14",
"Factors":{"Previus_COVID":"No","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_RA_T14","SEX":"female"}
},
{
"Subject ID":"17",
"Sample ID":"97-Serum_RA_T21",
"Factors":{"Previus_COVID":"No","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_RA_T21","SEX":"female"}
},
{
"Subject ID":"17",
"Sample ID":"98-Serum_RA_T28",
"Factors":{"Previus_COVID":"No","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_RA_T28","SEX":"female"}
},
{
"Subject ID":"17",
"Sample ID":"99-Serum_RA_T1M",
"Factors":{"Previus_COVID":"No","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_RA_T1M","SEX":"female"}
},
{
"Subject ID":"18",
"Sample ID":"100-Serum_SA_T0",
"Factors":{"Previus_COVID":"Yes","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SA_T0","SEX":"male"}
},
{
"Subject ID":"18",
"Sample ID":"101-Serum_SA_T7",
"Factors":{"Previus_COVID":"Yes","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SA_T07","SEX":"male"}
},
{
"Subject ID":"18",
"Sample ID":"102-Serum_SA_T14",
"Factors":{"Previus_COVID":"Yes","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SA_T14","SEX":"male"}
},
{
"Subject ID":"18",
"Sample ID":"103-Serum_SA_T21",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SA_T21","SEX":"male"}
},
{
"Subject ID":"18",
"Sample ID":"104-Serum_SA_T28",
"Factors":{"Previus_COVID":"Yes","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SA_T28","SEX":"male"}
},
{
"Subject ID":"18",
"Sample ID":"105-Serum_SA_T1M",
"Factors":{"Previus_COVID":"Yes","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SA_T1M","SEX":"male"}
},
{
"Subject ID":"19",
"Sample ID":"106-Serum_SB_T0",
"Factors":{"Previus_COVID":"Yes","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SB_T0","SEX":"female"}
},
{
"Subject ID":"19",
"Sample ID":"107-Serum_SB_T14",
"Factors":{"Previus_COVID":"Yes","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SB_T14","SEX":"female"}
},
{
"Subject ID":"19",
"Sample ID":"108-Serum_SB_T21",
"Factors":{"Previus_COVID":"Yes","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SB_T21","SEX":"female"}
},
{
"Subject ID":"19",
"Sample ID":"109-Serum_SB_T28",
"Factors":{"Previus_COVID":"Yes","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SB_T28","SEX":"female"}
},
{
"Subject ID":"19",
"Sample ID":"110-Serum_SB_T1M",
"Factors":{"Previus_COVID":"Yes","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_SB_T1M","SEX":"female"}
},
{
"Subject ID":"20",
"Sample ID":"111-Serum_VS_T0",
"Factors":{"Previus_COVID":"No","TIME":"T0"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_VS_T0","SEX":"female"}
},
{
"Subject ID":"20",
"Sample ID":"112-Serum_VS_T07",
"Factors":{"Previus_COVID":"No","TIME":"T7"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_VS_T07","SEX":"female"}
},
{
"Subject ID":"20",
"Sample ID":"113-Serum_VS_T14",
"Factors":{"Previus_COVID":"No","TIME":"T14"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_VS_T14","SEX":"female"}
},
{
"Subject ID":"20",
"Sample ID":"114-Serum_VS_T21",
"Factors":{"Previus_COVID":"No","TIME":"T21"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_VS_T21","SEX":"female"}
},
{
"Subject ID":"20",
"Sample ID":"115-Serum_VS_T28",
"Factors":{"Previus_COVID":"No","TIME":"T28"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_VS_T28","SEX":"female"}
},
{
"Subject ID":"20",
"Sample ID":"116-Serum_VS_T1M",
"Factors":{"Previus_COVID":"No","TIME":"T1M"},
"Additional sample data":{"RAW_FILE_NAME":"Siero_VAX_VS_T1M","SEX":"female"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"For all subjects blood serum samples were collected at six different time points: before the first dose (T0); 7 and 14 days after the first dose (T7 and T14, respectively); 21 days after the first dose, just before the second dose (T21); 28 days after the first dose and 7 days after the second dose (T28); 1 month after the second dose (T1M). Blood samples were collected (4 hours after breakfast) in a BD vacutainer clot-activator tube for serum collection and processed within one hour from sample collection. After processing, all the serum samples were immediately stored at -30°C until NMR analysis.","SAMPLE_TYPE":"Blood (serum)","STORAGE_CONDITIONS":"Described in summary","COLLECTION_VIALS":"BD vacutainer clot-activator tube for serum"},

"TREATMENT":{"TREATMENT_SUMMARY":"We monitor the effect of mRNA Pfizer-BioNTech vaccine in a cohort of 20 healthcare workers. All of them received two doses, 21 days apart.","TREATMENT":"Pfizer-BioNTech mRNA vaccine"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"NMR samples were prepared according to standard procedures. Frozen serum samples were thawed at room temperature. A total of 350 μL of sodium phosphate buffer (70 mM Na2HPO4; 20% (v/v) 2H2O; 6.1 mM NaN3, 4.6 mM sodium trimethylsilyl [2,2,3,3−2H4] propionate (TMSP), pH 7.4) was added to 350 μL of each serum sample; the mixture was homogenized by vortexing for 30 s. A total of 600 μL of each mixture was transferred into a 5.00 mm NMR tube (Bruker BioSpin) for the analysis.","PROCESSING_STORAGE_CONDITIONS":"-80℃"},

"ANALYSIS":{"ANALYSIS_TYPE":"NMR"},

"NM":{"INSTRUMENT_NAME":"Bruker","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","SPECTROMETER_FREQUENCY":"600MHz","NMR_PROBE":"5 mm PATXI 1H−13C−15N and 2H-decoupling probe","NMR_TUBE_SIZE":"5.00 mm","PULSE_SEQUENCE":"NOESY","WATER_SUPPRESSION":"1Dpresat","TEMPERATURE":"36,85","NUMBER_OF_SCANS":"32","DUMMY_SCANS":"4","ACQUISITION_TIME":"2.7 s","RELAXATION_DELAY":"4 s","SPECTRAL_WIDTH":"18,028 Hz","NUM_DATA_POINTS_ACQUIRED":"98,304","LINE_BROADENING":"0.3","BINNED_INCREMENT":"0.02 ppm","BINNED_DATA_EXCLUDED_RANGE":"4.5-5.5 ppm","NMR_RESULTS_FILE":"NOESY.txt UNITS:ppm"},

"NMR_METABOLITE_DATA":{
"Units":"mM",

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_T28":"291.11","70-Serum_MF_T1M":"275.87","71-Serum_MC_T0":"209.75","72-Serum_MC_T07":"234.54","73-Serum_MC_T14":"221.19","74-Serum_MC_T21":"206.15","75-Serum_MC_T28":"203.82","76-Serum_MC_T1M":"199.6","77-Serum_NF_T0":"257.37","78-Serum_NF_T7":"360.64","79-Serum_NF_T14":"289.11","80-Serum_NF_T21":"337.97","81-Serum_NF_T28":"336.01","82-Serum_NF_T1M":"303.77","83-Serum_PF_T0":"251.7","84-Serum_PF_T7":"199.49","85-Serum_PF_T14":"228.98","86-Serum_PF_T21":"237.38","87-Serum_PF_T1M":"211.12","88-Serum_PS_T0":"254.93","89-Serum_PS_T7":"224.72","90-Serum_PS_T14":"223.55","91-Serum_PS_T21":"227.24","92-Serum_PS_T28":"217.88","93-Serum_PS_T1M":"218.7","94-Serum_RA_T0":"230.38","95-Serum_RA_T07_250uL":"167.85","96-Serum_RA_T14":"216.25","97-Serum_RA_T21":"205.84","98-Serum_RA_T28":"194.2","99-Serum_RA_T1M":"145.59","100-Serum_SA_T0":"245.03","101-Serum_SA_T7":"236.57","102-Serum_SA_T14":"230.88","103-Serum_SA_T21":"277.39","104-Serum_SA_T28":"246.95","105-Serum_SA_T1M":"230.72","106-Serum_SB_T0":"222.12","107-Serum_SB_T14":"207.39","108-Serum_SB_T21":"220.92","109-Serum_SB_T28":"197.04","110-Serum_SB_T1M":"213.11","111-Serum_VS_T0":"163.7","112-Serum_VS_T07":"179.19","113-Serum_VS_T14":"144.49","114-Serum_VS_T21":"158.86","115-Serum_VS_T28":"190.22","116-Serum_VS_T1M":"157.58"},{"Metabolite":"LDL-Chol","1-Serum_AF_T0":"209.01","2-Serum_AF_T7":"208.9","3-Serum_AF_T14":"181.39","4-Serum_AF_T21":"187.02","5-Serum_AF_T28":"158.29","6-Serum_AF_T1M":"168.48","7-Serum_CI_T0":"118.92","8-Serum_CI_T07":"112.75","9-Serum_CI_T14":"85.94","10-Serum_CI_T21_200ul":"58.43","11-Serum_CI_T28":"82.46","12-Serum_CI_T1M":"82.29","13-Serum_CC_T07_250uL":"82.62","14-Serum_CC_T14_210uL":"86.37","15-Serum_CC_T21":"75.63","16-Serum_CC_T28":"101.15","17-Serum_CC_T0_250uL":"100.94","18-Serum_CC_T1M":"100.28","19-Serum_CCa_T07":"102.95","20-Serum_CCa_T14":"102.95","21-Serum_CCa_T21":"103.2","22-Serum_CCa_T28":"107.87","23-Serum_CCa_T0_210uL":"83.64","24-Serum_CCa_T1M":"109.79","25-Serum_CS_T0":"134.74","26-Serum_CS_T7":"152.39","27-Serum_CS_T14":"163.21","28-Serum_CS_T21":"154.47","29-Serum_CS_T28":"145.07","30-Serum_CMG_T0":"151.06","31-Serum_CMG_T7":"139.32","32-Serum_CMG_T14":"111.33","33-Serum_CMG_T21":"122.71","34-Serum_CMG_T28":"100.77","35-Serum_CMG_T1M":"128.85","36-Serum_CG_T0":"118.46","37-Serum_CG_T7":"138.43","38-Serum_CG_T14":"131.91","39-Serum_CG_T21":"145.79","40-Serum_CG_T28":"121.23","41-Serum_CG_T1M":"138.7","42-Serum_CA_T0":"178.36","43-Serum_CA_T7":"183.41","44-Serum_CA_T14":"166.24","45-Serum_CA_T21":"162.26","46-Serum_CA_T28":"167.12","47-Serum_CA_T1M":"147.65","48-Serum_DD_T0":"150.4","49-Serum_DD_T07":"150.34","50-Serum_DD_T21":"130.72","51-Serum_DD_T28":"99.06","52-Serum_DD_T1M":"127.96","53-Serum_GT_T0":"137.72","54-Serum_GT_T7":"138.24","55-Serum_GT_T14":"125.24","56-Serum_GT_T21":"112.73","57-Serum_GT_T28":"101.16","58-Serum_GT_T1M":"103.96","59-Serum_KT_T0":"149.34","60-Serum_KT_T07":"161.79","61-Serum_KT_T14":"164.68","62-Serum_KT_T21":"156.39","63-Serum_KT_T28_280ul":"155.23","64-Serum_KT_T1M":"127.8","65-Serum_MF_T0":"160.89","66-Serum_MF_T7":"180.57","67-Serum_MF_T14":"151.81","68-Serum_MF_T21":"127.44","69-Serum_MF_T28":"154.91","70-Serum_MF_T1M":"149.39","71-Serum_MC_T0":"117.03","72-Serum_MC_T07":"126.57","73-Serum_MC_T14":"117.08","74-Serum_MC_T21":"105.72","75-Serum_MC_T28":"110","76-Serum_MC_T1M":"110.51","77-Serum_NF_T0":"138.88","78-Serum_NF_T7":"197.83","79-Serum_NF_T14":"155.02","80-Serum_NF_T21":"141.27","81-Serum_NF_T28":"175.05","82-Serum_NF_T1M":"181.13","83-Serum_PF_T0":"118.31","84-Serum_PF_T7":"114.09","85-Serum_PF_T14":"119.41","86-Serum_PF_T21":"126.11","87-Serum_PF_T1M":"133.98","88-Serum_PS_T0":"157.01","89-Serum_PS_T7":"130.69","90-Serum_PS_T14":"128.89","91-Serum_PS_T21":"130.36","92-Serum_PS_T28":"123.11","93-Serum_PS_T1M":"129.97","94-Serum_RA_T0":"128.01","95-Serum_RA_T07_250uL":"101.04","96-Serum_RA_T14":"127.7","97-Serum_RA_T21":"104.05","98-Serum_RA_T28":"112.37","99-Serum_RA_T1M":"79.18","100-Serum_SA_T0":"148.67","101-Serum_SA_T7":"151.22","102-Serum_SA_T14":"121.61","103-Serum_SA_T21":"154.02","104-Serum_SA_T28":"149","105-Serum_SA_T1M":"139.79","106-Serum_SB_T0":"118.37","107-Serum_SB_T14":"104.33","108-Serum_SB_T21":"123.82","109-Serum_SB_T28":"100.83","110-Serum_SB_T1M":"116.07","111-Serum_VS_T0":"82.7","112-Serum_VS_T07":"81.3","113-Serum_VS_T14":"64.41","114-Serum_VS_T21":"84.78","115-Serum_VS_T28":"85.18","116-Serum_VS_T1M":"89.51"},{"Metabolite":"HDL-Chol","1-Serum_AF_T0":"85.42","2-Serum_AF_T7":"85.06","3-Serum_AF_T14":"80.18","4-Serum_AF_T21":"88.4","5-Serum_AF_T28":"71.06","6-Serum_AF_T1M":"71.69","7-Serum_CI_T0":"53.04","8-Serum_CI_T07":"57.82","9-Serum_CI_T14":"56.23","10-Serum_CI_T21_200ul":"40.57","11-Serum_CI_T28":"46.26","12-Serum_CI_T1M":"49.2","13-Serum_CC_T07_250uL":"49.94","14-Serum_CC_T14_210uL":"51.12","15-Serum_CC_T21":"46.76","16-Serum_CC_T28":"60.89","17-Serum_CC_T0_250uL":"56.85","18-Serum_CC_T1M":"58.37","19-Serum_CCa_T07":"67.49","20-Serum_CCa_T14":"7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}

}