{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002089","ANALYSIS_ID":"AN003413","VERSION":"1","CREATED_ON":"03-03-2022"},

"PROJECT":{"PROJECT_TITLE":"Plasma metabolomic signatures of COPD","INSTITUTE":"National Jewish Health","DEPARTMENT":"Division of Pulmonary Medicine","LAST_NAME":"Bowler","FIRST_NAME":"Russell","ADDRESS":"1400 Jackson St. Denver, CO 80206","EMAIL":"bowlerr@njhealth.org","PHONE":"303 270 2014","FUNDING_SOURCE":"NHLBI R21 HL140376, R01HL129937, R01HL 095432, R01 HL089856, R01 HL089897); and UL1 RR025680 from NCRR/HIH","DOI":"http://dx.doi.org/10.21228/M8FQ2X"},

"STUDY":{"STUDY_TITLE":"Plasma metabolomic signatures of COPD: A metabolomic severity score for airflow obstruction and emphysema.","STUDY_TYPE":"MS qualitative analysis","STUDY_SUMMARY":"The NIH sponsored multicenter Genetic Epidemiology of COPD (COPDGene (ClinicalTrials.gov Identifier: NCT01969344) study was approved and reviewed by the institutional review board at all participating centers (1). All study participants provided written informed consent. This study enrolled 10,198 non-Hispanic white (NHW) and African American (AA) individuals from January 2008 until April 2011 (Phase 1) who were aged 45-80 with ≥10 pack-year smoking history and no exacerbations for >30 days. In addition, 465 age and gender matched healthy individuals with no history of smoking were enrolled as controls (mostly at Phase 2). From July 2013 to July 2017, 5,697 subjects returned for an in-person 5-year visit. Each in-person visit included spirometry before and after albuterol, quantitative CT imaging of the chest, and blood sampling. From two clinical centers (National Jewish Health and University of Iowa) 1,136 subjects (1,040 NHW, 96 AA) participated in an ancillary study in which they provided fresh frozen plasma collected using an 8.5 ml p100 tube (Becton Dickinson) at Phase 2. This study is a duplicate of ST001443 (https://www.metabolomicsworkbench.org/data/DRCCMetadata.php?Mode=Study&StudyID=ST001443&StudyType=MS&ResultType=5) but differs in analysis and contains batch normalized data using median metabolite values.","INSTITUTE":"National Jewish Health","DEPARTMENT":"Division of Pulmonary Medicine","LABORATORY":"Bowler","LAST_NAME":"Bowler","FIRST_NAME":"Russell","ADDRESS":"1400 Jackson St. Denver, CO 80206","EMAIL":"bowlerr@njhealth.org","PHONE":"303 270 2014","SUBMIT_DATE":"2022-02-08"},

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{
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{
"Subject ID":"-",
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{
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{
"Subject ID":"-",
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{
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{
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{
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{
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"Subject ID":"-",
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{
"Subject ID":"-",
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{
"Subject ID":"-",
"Sample ID":"15811Q",
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{
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{
"Subject ID":"-",
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{
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{
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{
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{
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"COLLECTION":{"COLLECTION_SUMMARY":"The NIH sponsored multicenter Genetic Epidemiology of COPD (COPDGene (ClinicalTrials.gov Identifier: NCT01969344) study was approved and reviewed by the institutional review board at all participating centers (1). All study participants provided written informed consent. This study enrolled 10,198 non-Hispanic white (NHW) and African American (AA) individuals from January 2008 until April 2011 (Phase 1) who were aged 45-80 with ≥10 pack-year smoking history and no exacerbations for >30 days. In addition, 465 age and gender matched healthy individuals with no history of smoking were enrolled as controls (mostly at Phase 2). From July 2013 to July 2017, 5,697 subjects returned for an in-person 5-year visit. Each in-person visit included spirometry before and after albuterol, quantitative CT imaging of the chest, and blood sampling. From two clinical centers (National Jewish Health and University of Iowa) 1,136 subjects (1,040 NHW, 96 AA) participated in an ancillary study in which they provided fresh frozen plasma collected using an 8.5 ml p100 tube (Becton Dickinson) at Phase 2. Subject data includes: gender(Male/Female); race.eth (Non-Hispanic, White/Black African American/Other); smoking_status (Never smoked/Former Smoker/Current Smoker); ccenter (Clinical center); FEV1_post.2 (Post-bronchodilator, FEV1); adj_density_mesa (MESA-adjusted lung density), age_visit (Age years); BMI (Body Mass Index); ATS_PackYears (pack-years of smoking)","SAMPLE_TYPE":"Blood (plasma)"},

"TREATMENT":{"TREATMENT_SUMMARY":"The NIH sponsored multicenter Genetic Epidemiology of COPD (COPDGene (ClinicalTrials.gov Identifier: NCT01969344) study was approved and reviewed by the institutional review board at all participating centers (1). All study participants provided written informed consent. This study enrolled 10,198 non-Hispanic white (NHW) and African American (AA) individuals from January 2008 until April 2011 (Phase 1) who were aged 45-80 with ≥10 pack-year smoking history and no exacerbations for >30 days. In addition, 465 age and gender matched healthy individuals with no history of smoking were enrolled as controls (mostly at Phase 2). From July 2013 to July 2017, 5,697 subjects returned for an in-person 5-year visit. Each in-person visit included spirometry before and after albuterol, quantitative CT imaging of the chest, and blood sampling."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"P100 plasma was profiled using the Metabolon (Durham, USA) Global Metabolomics Platform, as described (2-4). Briefly, samples were extracted with methanol under vigorous shaking for two minutes (Glen Mills GenoGrinder 2000) followed by centrifugation to remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase/ultrahigh performance liquid chromatography/tandem mass spectrometry (RP/UPLC-MS/MS) methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by hydrophilic interaction chromatography (HILIC)/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"HILIC","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)","CHROMATOGRAPHY_TYPE":"HILIC"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo LTQ","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","MS_COMMENTS":"Proprietary analytical software for integration and peak picking","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"relativeMedian",

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