{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002182","ANALYSIS_ID":"AN003574","VERSION":"1","CREATED_ON":"June 2, 2022, 6:00 pm"},

"PROJECT":{"PROJECT_TITLE":"Protective effects of maternal PQQ on hepatic lipid metabolism throughout the lifespan","PROJECT_TYPE":"Diet study and fetal programming","PROJECT_SUMMARY":"Maternal obesity and consumption of a high-fat diet significantly elevate risk for pediatric non-alcoholic fatty liver disease (NAFLD), affecting 10% of children in the US. Almost half of these children are diagnosed with nonalcoholic steatohepatitis (NASH), a leading etiology for liver transplant. Animal models show that signs of liver injury and perturbed lipid metabolism asso-ciated with NAFLD begin in utero; however, safe dietary therapeutics to blunt developmental programming of NAFLD are unavailable. Using a mouse model of maternal Western-style diet (WD), we previously showed that pyrroloquinoline quinone (PQQ), a potent dietary antioxidant, protected offspring of WD-fed dams from development of NAFLD and NASH. Here, we used untargeted mass spectrometry-based lipidomics to delineate lipotoxic effects of WD on offspring liver and identify lipid targets of PQQ. PQQ exposure during pregnancy altered hepatic lipid profiles of WD-exposed offspring, upregulating peroxisome proliferator-activated receptor (PPAR) α signaling and mitochondrial fatty acid oxidation to markedly attenuate triglyceride accumulation beginning in utero. Surprisingly, the abundance of very long-chain ceramides, important in promoting gut barrier and hepatic function, was significantly elevated in PQQ-treated offspring. PQQ exposure reduced the hepatic phosphatidylcho-line/phosphatidylethanolamine (PC/PE) ratio in WD-fed offspring and improved glucose toler-ance. Notably, levels of protective n − 3 polyunsaturated fatty acids (PUFAs) were elevated in offspring exposed to PQQ, beginning in utero, and the increase in n − 3 PUFAs persisted into adulthood. Our findings suggest that PQQ supplementation during gestation and lactation augments pathways involved in the biosynthesis of long-chain fatty acids and plays a unique role in modifying specific bioactive lipid species critical for protection against NAFLD risk in later life.","INSTITUTE":"University of Oklahoma Health Sciences Center","DEPARTMENT":"Biochemistry and Molecular Biology, Harold Hamm Diabetes Center","LABORATORY":"Jonscher","LAST_NAME":"Jonscher","FIRST_NAME":"Karen","ADDRESS":"975 NE 10th Street BRC-N 362A, Oklahoma City, OK, 73104, USA","EMAIL":"karen-jonscher@ouhsc.edu","PHONE":"3032294620","FUNDING_SOURCE":"NIDDK"},

"STUDY":{"STUDY_TITLE":"Amelioration of developmental programming of NAFLD in weanling liver using PQQ","STUDY_TYPE":"Diet and PQQ treatment","STUDY_SUMMARY":"Maternal obesity and consumption of a high-fat diet significantly elevate risk for pediatric non-alcoholic fatty liver disease (NAFLD), affecting 10% of children in the US. Almost half of these children are diagnosed with nonalcoholic steatohepatitis (NASH), a leading etiology for liver transplant. Animal models show that signs of liver injury and perturbed lipid metabolism asso-ciated with NAFLD begin in utero; however, safe dietary therapeutics to blunt developmental programming of NAFLD are unavailable. Using a mouse model of maternal Western-style diet (WD), we previously showed that pyrroloquinoline quinone (PQQ), a potent dietary antioxidant, protected offspring of WD-fed dams from development of NAFLD and NASH. Here, we used untargeted mass spectrometry-based lipidomics to delineate lipotoxic effects of WD on offspring liver and identify lipid targets of PQQ. PQQ exposure during pregnancy altered hepatic lipid profiles of WD-exposed offspring, upregulating peroxisome proliferator-activated receptor (PPAR) α signaling and mitochondrial fatty acid oxidation to markedly attenuate triglyceride accumulation beginning in utero. Surprisingly, the abundance of very long-chain ceramides, important in promoting gut barrier and hepatic function, was significantly elevated in PQQ-treated offspring. PQQ exposure reduced the hepatic phosphatidylcho-line/phosphatidylethanolamine (PC/PE) ratio in WD-fed offspring and improved glucose toler-ance. Notably, levels of protective n − 3 polyunsaturated fatty acids (PUFAs) were elevated in offspring exposed to PQQ, beginning in utero, and the increase in n − 3 PUFAs persisted into adulthood. Our findings suggest that PQQ supplementation during gestation and lactation augments pathways involved in the biosynthesis of long-chain fatty acids and plays a unique role in modifying specific bioactive lipid species critical for protection against NAFLD risk in later life.","INSTITUTE":"University of Oklahoma Health Sciences Center","DEPARTMENT":"Biochemistry and Molecular Biology, Harold Hamm Diabetes Center","LABORATORY":"Jonscher","LAST_NAME":"Jonscher","FIRST_NAME":"Karen","ADDRESS":"975 NE 10th Street BRC-N 362A, Oklahoma City, OK, 73104, USA","EMAIL":"karen-jonscher@ouhsc.edu","PHONE":"3032294620","NUM_GROUPS":"3","TOTAL_SUBJECTS":"9","PUBLICATIONS":"Jonscher, et al FASEB J 2017; Friedman, et al Hepatol Commun 2018"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","AGE_OR_AGE_RANGE":"3-4 weeks","GENDER":"Male","ANIMAL_HOUSING":"Vivarium University of Colorado Anschutz Medical Campus","ANIMAL_LIGHT_CYCLE":"12/12","ANIMAL_FEED":"CH; 2019; Envigo, Indianapolis, IN or .D  TD.88137; Envigo","ANIMAL_WATER":"Water or treated with 1.25 mg/L PQQ"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"639.4",
"Factors":{"Diet":"CH"}
},
{
"Subject ID":"-",
"Sample ID":"639.2",
"Factors":{"Diet":"CH"}
},
{
"Subject ID":"-",
"Sample ID":"639.1",
"Factors":{"Diet":"CH"}
},
{
"Subject ID":"-",
"Sample ID":"399.5",
"Factors":{"Diet":"WD"}
},
{
"Subject ID":"-",
"Sample ID":"526.1",
"Factors":{"Diet":"WD"}
},
{
"Subject ID":"-",
"Sample ID":"526.3",
"Factors":{"Diet":"WD"}
},
{
"Subject ID":"-",
"Sample ID":"514.1",
"Factors":{"Diet":"WDPQQ"}
},
{
"Subject ID":"-",
"Sample ID":"183.1",
"Factors":{"Diet":"WDPQQ"}
},
{
"Subject ID":"-",
"Sample ID":"183.4",
"Factors":{"Diet":"WDPQQ"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Livers were excised and snap frozen then sent to the Metabolomics Core at the University of Colorado Anschutz Medical Campus for analysis following standard protocols.","SAMPLE_TYPE":"Liver","STORAGE_CONDITIONS":"-80℃","COLLECTION_VIALS":"Cryotubes","STORAGE_VIALS":"Cryotubes"},

"TREATMENT":{"TREATMENT_SUMMARY":"Dams were fed either chow (CH) or Western-style diet (WD), with or without PQQ in drinking water.","TREATMENT":"WD and PQQ","TREATMENT_COMPOUND":"BioPQQ","TREATMENT_ROUTE":"Drinking water, ad libitem","TREATMENT_DOSE":"1.25 mg/L","TREATMENT_DOSEDURATION":"6-7 weeks","TREATMENT_VEHICLE":"drinking water"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Tissue was homogenized and lipids extracted following standard protocols at the Metabolomics Core at the University of Colorado Anschutz Medical Campus.","SAMPLEPREP_PROTOCOL_FILENAME":"Fetal_and_weanling_lipid_protocols.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"The analytical platform employed a Vanquish UHPLC system (Thermo Fisher Scientific, San Jose, CA, USA) coupled online to a Q Exactive mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). Lipidomics analyses were performed as described in previous method papers and application notes. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640979/ https://www.haematologica.org/article/view/9990, https://www.haematologica.org/article/view/9990 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640979/, https://pubmed.ncbi.nlm.nih.gov/31119660/","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Waters Acquity BEH HSS T3 (100 x 2.1mm, 1.8um)","METHODS_FILENAME":"Fetal_and_weanling_lipid_protocols.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"Fetal_and_weanling_lipid_protocols.pdf"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"See attached file"},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

"Data":[{"Metabolite":"Glycerol 3-phosphate","639.4":"3.40E+07","639.2":"4.05E+07","639.1":"3.00E+07","399.5":"4.55E+07","526.1":"3.11E+07","526.3":"3.23E+07","514.1":"1.19E+07","183.1":"4.22E+07","183.4":"2.81E+07"},{"Metabolite":"Ethanolamine phosphate","639.4":"2.05E+06","639.2":"1.81E+06","639.1":"2.36E+06","399.5":"7.14E+06","526.1":"4.48E+06","526.3":"3.41E+06","514.1":"3.62E+06","183.1":"3.39E+06","183.4":"4.32E+06"},{"Metabolite":"Sphingosine","639.4":"9.67E+04","639.2":"8.09E+03","639.1":"4.09E+03","399.5":"1.32E+06","526.1":"1.01E+04","526.3":"1.74E+04","514.1":"8.44E+03","183.1":"1.97E+04","183.4":"1.69E+04"},{"Metabolite":"sn-glycero-3-Phosphoethanolamine","639.4":"4.59E+06","639.2":"3.58E+06","639.1":"3.29E+06","399.5":"4.34E+07","526.1":"1.73E+07","526.3":"1.68E+07","514.1":"3.35E+07","183.1":"4.12E+07","183.4":"3.45E+07"},{"Metabolite":"Acetylcholine","639.4":"2.47E+07","639.2":"2.39E+07","639.1":"1.93E+07","399.5":"5.39E+06","526.1":"8.15E+06","526.3":"8.86E+06","514.1":"6.48E+06","183.1":"6.40E+06","183.4":"4.51E+06"},{"Metabolite":"acetyl-carnitine","639.4":"1.84E+08","639.2":"1.36E+08","639.1":"1.56E+08","399.5":"5.81E+07","526.1":"6.92E+07","526.3":"8.83E+07","514.1":"5.14E+07","183.1":"5.02E+07","183.4":"2.46E+07"},{"Metabolite":"propionyl-carnitine","639.4":"2.94E+07","639.2":"3.18E+07","639.1":"3.11E+07","399.5":"1.94E+06","526.1":"9.29E+06","526.3":"6.65E+06","514.1":"2.89E+06","183.1":"1.20E+06","183.4":"1.79E+06"},{"Metabolite":"butanoyl-l-carnitine","639.4":"9.31E+06","639.2":"2.32E+07","639.1":"7.13E+06","399.5":"9.44E+05","526.1":"3.05E+06","526.3":"4.41E+06","514.1":"6.13E+05","183.1":"1.22E+06","183.4":"9.19E+05"},{"Metabolite":"acyl-C4-OH","639.4":"1.06E+07","639.2":"8.31E+06","639.1":"9.45E+06","399.5":"5.47E+06","526.1":"5.04E+06","526.3":"5.80E+06","514.1":"4.58E+06","183.1":"3.97E+06","183.4":"2.90E+06"},{"Metabolite":"acyl-C5","639.4":"1.43E+07","639.2":"1.66E+07","639.1":"1.19E+07","399.5":"8.25E+05","526.1":"1.64E+06","526.3":"9.97E+05","514.1":"4.51E+05","183.1":"7.49E+04","183.4":"8.05E+05"},{"Metabolite":"acyl-C5:1","639.4":"9.63E+04","639.2":"1.01E+05","639.1":"1.15E+05","399.5":"4.66E+04","526.1":"3.60E+04","526.3":"3.14E+04","514.1":"5.92E+04","183.1":"3.41E+04","183.4":"3.55E+04"},{"Metabolite":"acyl-C5-OH","639.4":"2.98E+06","639.2":"3.22E+06","639.1":"2.81E+06","399.5":"3.68E+05","526.1":"8.50E+05","526.3":"9.58E+05","514.1":"6.13E+05","183.1":"4.50E+05","183.4":"3.92E+05"},{"Metabolite":"hexanoyl-L-carnitine","639.4":"5.54E+06","639.2":"1.64E+07","639.1":"3.61E+06","399.5":"7.53E+05","526.1":"1.55E+06","526.3":"1.46E+06","514.1":"2.68E+05","183.1":"3.49E+05","183.4":"4.69E+05"},{"Metabolite":"L-octanoylcarnitine","639.4":"1.23E+06","639.2":"4.29E+06","639.1":"6.82E+05","399.5":"3.06E+05","526.1":"6.17E+05","526.3":"5.85E+05","514.1":"1.89E+05","183.1":"2.30E+05","183.4":"1.66E+05"},{"Metabolite":"octenoyl-l-carnitine","639.4":"5.37E+05","639.2":"4.27E+05","639.1":"3.34E+05","399.5":"1.99E+04","526.1":"4.89E+04","526.3":"2.48E+04","514.1":"2.69E+04","183.1":"8.67E+03","183.4":"1.31E+04"},{"Metabolite":"O-Decanoyl-L-carnitine","639.4":"1.44E+05","639.2":"6.44E+05","639.1":"1.58E+05","399.5":"3.03E+03","526.1":"3.66E+05","526.3":"4.43E+05","514.1":"1.18E+05","183.1":"4.90E+04","183.4":"2.82E+03"},{"Metabolite":"O-Decenoyl-L-carnitine","639.4":"4.13E+05","639.2":"2.77E+05","639.1":"2.54E+05","399.5":"9.24E+04","526.1":"3.49E+05","526.3":"5.25E+05","514.1":"1.16E+05","183.1":"1.79E+05","183.4":"7.54E+04"},{"Metabolite":"O-dodecenoyl-carnitine","639.4":"8.87E+02","639.2":"9.11E+03","639.1":"1.05E+04","399.5":"0.00E+00","526.1":"6.50E+04","526.3":"6.83E+04","514.1":"3.17E+04","183.1":"8.15E+02","183.4":"7.71E+02"},{"Metabolite":"acyl-C18:2","639.4":"1.02E+04","639.2":"6.81E+03","639.1":"6.27E+03","399.5":"1.03E+04","526.1":"2.06E+03","526.3":"1.19E+04","514.1":"3.39E+03","183.1":"6.18E+05","183.4":"5.24E+03"},{"Metabolite":"Hexanoic acid (caproate)","639.4":"1.62E+05","639.2":"2.40E+05","639.1":"1.53E+05","399.5":"1.14E+05","526.1":"1.50E+05","526.3":"1.66E+05","514.1":"1.66E+05","183.1":"1.39E+05","183.4":"1.37E+05"},{"Metabolite":"Heptanoic acid","639.4":"3.58E+05","639.2":"4.19E+05","639.1":"3.52E+05","399.5":"2.43E+05","526.1":"3.34E+05","526.3":"3.28E+05","514.1":"3.23E+05","183.1":"2.14E+05","183.4":"2.84E+05"},{"Metabolite":"Octanoic acid (caprylate)","639.4":"1.85E+06","639.2":"2.02E+06","639.1":"1.51E+06","399.5":"1.44E+06","526.1":"1.81E+06","526.3":"1.62E+06","514.1":"1.66E+06","183.1":"1.35E+06","183.4":"1.37E+06"},{"Metabolite":"Nonanoic acid (pelargonate)","639.4":"1.78E+07","639.2":"1.79E+07","639.1":"1.36E+07","399.5":"1.11E+07","526.1":"1.35E+07","526.3":"1.52E+07","514.1":"1.34E+07","183.1":"9.38E+06","183.4":"1.16E+07"},{"Metabolite":"Decanoic acid (caprate)","639.4":"8.87E+06","639.2":"8.92E+06","639.1":"7.34E+06","399.5":"6.54E+06","526.1":"8.69E+06","526.3":"7.78E+06","514.1":"7.59E+06","183.1":"6.06E+06","183.4":"6.38E+06"},{"Metabolite":"Dodecanoic acid","639.4":"3.86E+06","639.2":"4.36E+06","639.1":"3.26E+06","399.5":"4.89E+06","526.1":"4.73E+06","526.3":"3.70E+06","514.1":"4.78E+06","183.1":"4.40E+06","183.4":"5.16E+06"},{"Metabolite":"Tetradecanoic acid","639.4":"8.31E+06","639.2":"8.84E+06","639.1":"8.37E+06","399.5":"2.01E+07","526.1":"1.26E+07","526.3":"7.56E+06","514.1":"2.07E+07","183.1":"1.58E+07","183.4":"2.18E+07"},{"Metabolite":"Hexadecanoic acid","639.4":"9.59E+07","639.2":"7.36E+07","639.1":"9.34E+07","399.5":"1.16E+08","526.1":"7.49E+07","526.3":"7.58E+07","514.1":"1.12E+08","183.1":"9.02E+07","183.4":"1.32E+08"},{"Metabolite":"Octadecanoic acid","639.4":"1.31E+07","639.2":"1.11E+07","639.1":"3.40E+05","399.5":"1.12E+07","526.1":"1.23E+07","526.3":"3.32E+05","514.1":"0.00E+00","183.1":"1.15E+07","183.4":"4.69E+05"},{"Metabolite":"Tetradecenoic acid","639.4":"1.10E+05","639.2":"6.91E+04","639.1":"1.01E+05","399.5":"3.47E+06","526.1":"1.06E+06","526.3":"6.88E+05","514.1":"3.36E+06","183.1":"2.16E+06","183.4":"3.21E+06"},{"Metabolite":"Hexadecenoic acid","639.4":"3.07E+06","639.2":"2.37E+06","639.1":"3.12E+06","399.5":"6.10E+07","526.1":"1.77E+07","526.3":"8.71E+06","514.1":"4.18E+07","183.1":"3.65E+07","183.4":"4.92E+07"},{"Metabolite":"Octadecenoic acid","639.4":"2.93E+07","639.2":"2.11E+07","639.1":"2.88E+07","399.5":"1.24E+08","526.1":"6.28E+07","526.3":"4.19E+07","514.1":"1.20E+08","183.1":"1.17E+08","183.4":"1.49E+08"},{"Metabolite":"Linoleate","639.4":"6.94E+07","639.2":"4.85E+07","639.1":"3.45E+07","399.5":"2.04E+07","526.1":"9.59E+06","526.3":"3.61E+06","514.1":"1.85E+07","183.1":"1.13E+07","183.4":"1.86E+07"},{"Metabolite":"Octadecatrienoic acid","639.4":"1.01E+07","639.2":"7.52E+06","639.1":"4.14E+06","399.5":"1.97E+06","526.1":"5.59E+05","526.3":"2.88E+05","514.1":"1.26E+06","183.1":"1.91E+06","183.4":"2.02E+06"},{"Metabolite":"Eicosapentaenoic acid","639.4":"3.61E+06","639.2":"2.75E+06","639.1":"1.83E+06","399.5":"2.69E+06","526.1":"1.67E+06","526.3":"8.13E+05","514.1":"1.73E+06","183.1":"3.69E+06","183.4":"5.01E+06"},{"Metabolite":"Docosahexaenoic acid","639.4":"1.17E+07","639.2":"9.10E+06","639.1":"7.38E+06","399.5":"2.23E+07","526.1":"1.55E+07","526.3":"7.19E+06","514.1":"1.76E+07","183.1":"2.57E+07","183.4":"3.06E+07"},{"Metabolite":"Dodecanedioic acid","639.4":"1.90E+05","639.2":"1.44E+05","639.1":"1.38E+05","399.5":"1.55E+05","526.1":"1.07E+05","526.3":"8.74E+04","514.1":"7.04E+04","183.1":"9.11E+04","183.4":"9.44E+04"},{"Metabolite":"(8Z-11Z-14Z)-Icosatrienoic acid","639.4":"1.78E+06","639.2":"1.29E+06","639.1":"1.18E+06","399.5":"3.29E+06","526.1":"1.88E+06","526.3":"8.93E+03","514.1":"3.24E+06","183.1":"2.94E+06","183.4":"3.64E+06"},{"Metabolite":"(5Z-8Z-11Z-14Z-17Z)-Icosapentaenoic acid","639.4":"3.61E+06","639.2":"2.75E+06","639.1":"1.83E+06","399.5":"2.69E+06","526.1":"1.67E+06","526.3":"8.13E+05","514.1":"1.73E+06","183.1":"3.69E+06","183.4":"5.01E+06"},{"Metabolite":"(7Z-10Z-13Z-16Z-19Z)-Docosa-7-10-13-16-19-pentaenoic acid","639.4":"1.43E+06","639.2":"8.42E+05","639.1":"8.44E+05","399.5":"3.32E+06","526.1":"1.92E+06","526.3":"7.09E+05","514.1":"2.52E+06","183.1":"1.26E+06","183.4":"1.87E+06"},{"Metabolite":"alpha-tocopheronolactone","639.4":"2.37E+05","639.2":"2.26E+05","639.1":"2.17E+05","399.5":"2.22E+05","526.1":"1.79E+05","526.3":"1.72E+05","514.1":"1.45E+05","183.1":"2.04E+05","183.4":"2.41E+05"},{"Metabolite":"dihydroxy-octadecanoic acid","639.4":"3.94E+05","639.2":"2.74E+05","639.1":"3.55E+05","399.5":"4.62E+05","526.1":"2.59E+05","526.3":"2.60E+05","514.1":"3.74E+05","183.1":"3.47E+05","183.4":"5.39E+05"},{"Metabolite":"hydroxy-dodecanedioic acid","639.4":"2.47E+04","639.2":"1.59E+04","639.1":"1.45E+04","399.5":"1.68E+04","526.1":"9.42E+03","526.3":"7.08E+03","514.1":"9.60E+03","183.1":"1.18E+04","183.4":"1.89E+04"},{"Metabolite":"dihydroxy-hexadecanoic acid","639.4":"8.92E+04","639.2":"6.17E+04","639.1":"7.40E+04","399.5":"1.88E+05","526.1":"7.60E+04","526.3":"3.38E+04","514.1":"6.06E+04","183.1":"1.33E+05","183.4":"1.95E+05"},{"Metabolite":"trihydroxy-octadecenoic acid","639.4":"6.43E+05","639.2":"7.45E+05","639.1":"8.12E+05","399.5":"5.22E+05","526.1":"8.47E+05","526.3":"8.78E+05","514.1":"9.05E+05","183.1":"8.02E+05","183.4":"7.84E+05"},{"Metabolite":"epoxy-octadecanoic acid","639.4":"2.79E+04","639.2":"3.76E+04","639.1":"2.72E+04","399.5":"1.28E+04","526.1":"2.68E+04","526.3":"2.55E+04","514.1":"3.17E+04","183.1":"1.89E+04","183.4":"2.68E+04"},{"Metabolite":"Taurocholate","639.4":"2.79E+06","639.2":"4.13E+06","639.1":"4.20E+06","399.5":"1.60E+06","526.1":"2.33E+06","526.3":"1.24E+06","514.1":"2.04E+06","183.1":"9.93E+05","183.4":"1.98E+06"},{"Metabolite":"Taurochenodeoxycholate","639.4":"6.57E+06","639.2":"7.44E+06","639.1":"9.43E+06","399.5":"5.23E+06","526.1":"4.45E+06","526.3":"2.53E+06","514.1":"1.19E+06","183.1":"2.15E+06","183.4":"2.51E+06"},{"Metabolite":"3alpha-12alpha-Dihydroxy-5beta-cholanate","639.4":"4.33E+04","639.2":"1.05E+05","639.1":"4.88E+04","399.5":"2.08E+04","526.1":"1.49E+04","526.3":"1.17E+04","514.1":"1.30E+04","183.1":"1.37E+04","183.4":"2.75E+04"},{"Metabolite":"Sulfoglycolithocholate","639.4":"7.73E+03","639.2":"1.32E+04","639.1":"2.28E+04","399.5":"1.13E+05","526.1":"6.44E+04","526.3":"1.10E+05","514.1":"5.67E+04","183.1":"1.77E+05","183.4":"1.09E+05"},{"Metabolite":"13(S)-HODE","639.4":"1.30E+05","639.2":"1.13E+05","639.1":"1.11E+05","399.5":"5.96E+04","526.1":"4.05E+04","526.3":"3.60E+04","514.1":"4.06E+04","183.1":"3.61E+04","183.4":"5.70E+04"},{"Metabolite":"11(R)-HPETE/Leukotriene 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"Metabolites":[{"metabolite_name":"Glycerol 3-phosphate"},{"metabolite_name":"Ethanolamine phosphate"},{"metabolite_name":"Sphingosine"},{"metabolite_name":"sn-glycero-3-Phosphoethanolamine"},{"metabolite_name":"Acetylcholine"},{"metabolite_name":"acetyl-carnitine"},{"metabolite_name":"propionyl-carnitine"},{"metabolite_name":"butanoyl-l-carnitine"},{"metabolite_name":"acyl-C4-OH"},{"metabolite_name":"acyl-C5"},{"metabolite_name":"acyl-C5:1"},{"metabolite_name":"acyl-C5-OH"},{"metabolite_name":"hexanoyl-L-carnitine"},{"metabolite_name":"L-octanoylcarnitine"},{"metabolite_name":"octenoyl-l-carnitine"},{"metabolite_name":"O-Decanoyl-L-carnitine"},{"metabolite_name":"O-Decenoyl-L-carnitine"},{"metabolite_name":"O-dodecenoyl-carnitine"},{"metabolite_name":"acyl-C18:2"},{"metabolite_name":"Hexanoic acid (caproate)"},{"metabolite_name":"Heptanoic acid"},{"metabolite_name":"Octanoic acid (caprylate)"},{"metabolite_name":"Nonanoic acid (pelargonate)"},{"metabolite_name":"Decanoic acid (caprate)"},{"metabolite_name":"Dodecanoic acid"},{"metabolite_name":"Tetradecanoic acid"},{"metabolite_name":"Hexadecanoic acid"},{"metabolite_name":"Octadecanoic acid"},{"metabolite_name":"Tetradecenoic acid"},{"metabolite_name":"Hexadecenoic acid"},{"metabolite_name":"Octadecenoic acid"},{"metabolite_name":"Linoleate"},{"metabolite_name":"Octadecatrienoic acid"},{"metabolite_name":"Eicosapentaenoic acid"},{"metabolite_name":"Docosahexaenoic acid"},{"metabolite_name":"Dodecanedioic acid"},{"metabolite_name":"(8Z-11Z-14Z)-Icosatrienoic acid"},{"metabolite_name":"(5Z-8Z-11Z-14Z-17Z)-Icosapentaenoic acid"},{"metabolite_name":"(7Z-10Z-13Z-16Z-19Z)-Docosa-7-10-13-16-19-pentaenoic acid"},{"metabolite_name":"alpha-tocopheronolactone"},{"metabolite_name":"dihydroxy-octadecanoic acid"},{"metabolite_name":"hydroxy-dodecanedioic acid"},{"metabolite_name":"dihydroxy-hexadecanoic acid"},{"metabolite_name":"trihydroxy-octadecenoic acid"},{"metabolite_name":"epoxy-octadecanoic acid"},{"metabolite_name":"Taurocholate"},{"metabolite_name":"Taurochenodeoxycholate"},{"metabolite_name":"3alpha-12alpha-Dihydroxy-5beta-cholanate"},{"metabolite_name":"Sulfoglycolithocholate"},{"metabolite_name":"13(S)-HODE"},{"metabolite_name":"11(R)-HPETE/Leukotriene B4 (isobars)"},{"metabolite_name":"Prostaglandin D2/Thromboxane A2 (isobars)"},{"metabolite_name":"Prostaglandin D3 (isobars)"},{"metabolite_name":"Prostaglandin G2"},{"metabolite_name":"2-3-Dinor-8-iso prostaglandin F2alpha"},{"metabolite_name":"Cholesterol sulfate"},{"metabolite_name":"Cortisol"},{"metabolite_name":"Cortisone"},{"metabolite_name":"Mesterolone"},{"metabolite_name":"N-hexadecanoyl-ethanolamine"},{"metabolite_name":"N-(Hexadecanoyl)-ethanolamine"},{"metabolite_name":"N-(5Z_8Z_11Z_14Z-docosatetraenoyl)-ethanolamine"},{"metabolite_name":"N-(11Z_14Z-eicosadienoyl)-ethanolamine"},{"metabolite_name":"N-(11Z-eicosaenoyl)-ethanolamine"},{"metabolite_name":"1-octadecanoyl-sn-glycero-3-phosphoethanolamine"},{"metabolite_name":"1-hexadecanoyl-sn-glycero-3-phosphoethanolamine"},{"metabolite_name":"1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine"},{"metabolite_name":"1-(5Z_8Z_11Z_14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine"},{"metabolite_name":"1-(1Z-hexadecenyl)-sn-glycero-3-phosphoethanolamine"},{"metabolite_name":"3-pentadecanoyl-sn-glycero-1-phosphocholine"},{"metabolite_name":"3-hexadecanoyl-sn-glycero-1-phosphocholine"},{"metabolite_name":"2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine"},{"metabolite_name":"2-(5Z_8Z_11Z_14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine"}]
}

}