{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002204","ANALYSIS_ID":"AN003607","VERSION":"1","CREATED_ON":"June 29, 2022, 11:00 am"},

"PROJECT":{"PROJECT_TITLE":"Endothelial Sirtuin1 Suppresses Whole-body Insulin Sensitivity by Modulating the Secretome","PROJECT_TYPE":"Profiling metabolites in skeletal muscle","PROJECT_SUMMARY":"Profiling metabolites in skeletal muscle from wild type vs. endothelium-specific Sirt1 knockout (E-Sirt1-KO) mice.","INSTITUTE":"University of Iowa","DEPARTMENT":"Internal medicine","LABORATORY":"Irani lab","LAST_NAME":"Irani","FIRST_NAME":"Kaikobad","ADDRESS":"RM 2256 CBRB, Newton Rd, Iowa City, IA 52242","EMAIL":"kaikobad-irani@uiowa.edu","PHONE":"319-335-8821","FUNDING_SOURCE":"AHA Award #20CDA35320042"},

"STUDY":{"STUDY_TITLE":"Endothelial Sirtuin1 Suppresses Whole-body Insulin Sensitivity by Modulating the Secretome","STUDY_TYPE":"End point","STUDY_SUMMARY":"Sirtuin1 (Sirt1) in skeletal muscle (SK) and fat protects against metabolic damage by stimulating insulin sensitivity. Here we report that mice with selective deletion of endothelial Sirt1 (E-Sirt1-KO) paradoxically exhibit heightened whole-body insulin sensitivity. Akt phosphorylation, glucose uptake, and glycolysis are boosted in SK and brown adipose tissue (BAT) of E-Sirt1-KO mice. E-Sirt1-KO mice have higher energy expenditure and are partially protected from high-fat diet-induced insulin resistance. Enhanced insulin sensitivity and peripheral tissue Akt phosphorylation in E-Sirt1-KO mice is transferrable to wild-type mice via the systemic circulation after surgical parabiosis. Silencing of Sirt1 in endothelial cells upregulates transcription of the F-actin-binding protein thymosin beta-4 (Tβ4), whose secretion activates Akt in skeletal myotubes. Sirt1 downregulation stimulates endothelial Tβ4 transcription through inhibition of autophagy and upregulation of nuclear factor-kappa B signaling. Thus, unlike Sirt1 in skeletal muscle and fat, endothelial Sirt1 curtails whole-body insulin sensitivity by inhibiting expression of secreted Tβ4","INSTITUTE":"University of Iowa","DEPARTMENT":"Internal medicine","LABORATORY":"irani","LAST_NAME":"Irani","FIRST_NAME":"Kaikobad","ADDRESS":"RM 2256 CBRB, Newton Rd, Iowa City, IA 52242","EMAIL":"kaikobad-irani@uiowa.edu","PHONE":"3193358821"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"wild type and endothelium-specific Sirt1 knockout mice","AGE_OR_AGE_RANGE":"12 weeks old","WEIGHT_OR_WEIGHT_RANGE":"~30 g","HEIGHT_OR_HEIGHT_RANGE":"n/a","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"Jax","ANIMAL_HOUSING":"University of Iowa animal house","ANIMAL_LIGHT_CYCLE":"12 h light/12 h night","ANIMAL_FEED":"Standard Chow","ANIMAL_WATER":"Tap water","ANIMAL_INCLUSION_CRITERIA":"n/a","SPECIES_GROUP":"n/a"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"S1",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"00112_KI_QL_20190322_ISQ_SIM_0100_WT_05.cdf"}
},
{
"Subject ID":"-",
"Sample ID":"S2",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"00112_KI_QL_20190322_ISQ_SIM_0100_WT_08.cdf"}
},
{
"Subject ID":"-",
"Sample ID":"S3",
"Factors":{"Genotype":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"00112_KI_QL_20190322_ISQ_SIM_0100_WT_11.cdf"}
},
{
"Subject ID":"-",
"Sample ID":"S4",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"00112_KI_QL_20190322_ISQ_SIM_0100_KO_06.cdf"}
},
{
"Subject ID":"-",
"Sample ID":"S5",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"00112_KI_QL_20190322_ISQ_SIM_0100_KO_07.cdf"}
},
{
"Subject ID":"-",
"Sample ID":"S6",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"00112_KI_QL_20190322_ISQ_SIM_0100_KO_09.cdf"}
},
{
"Subject ID":"-",
"Sample ID":"S7",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"00112_KI_QL_20190322_ISQ_SIM_0100_KO_10.cdf"}
},
{
"Subject ID":"-",
"Sample ID":"S8",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"00112_KI_QL_20190322_ISQ_SIM_0100_KO_12.cdf"}
},
{
"Subject ID":"-",
"Sample ID":"S9",
"Factors":{"Genotype":"KO"},
"Additional sample data":{"RAW_FILE_NAME":"00112_KI_QL_20190322_ISQ_SIM_0100_KO_13.cdf"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"40 mg of tibialis anterior was rapidly harvested and frozen immediately in liquid nitrogen. Metabolites were separated, measured, and analyzed.","SAMPLE_TYPE":"Muscle"},

"TREATMENT":{"TREATMENT_SUMMARY":"After 5 to 6 h of fasting, mice were anesthetized and skeletal muscle were collected."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Tissue samples were lyophilized overnight prior to bead mill homogenization in 18:1 (µl:mg wet tissue weight) ice-cold 2:2:1 methanol:acetonitrile:water extraction buffer containing a mixture of internal standards (D4-citric acid, D4-succinic acid, D8-valine, and U13C-labeled glutamine, glutamic acid, lysine, methionine, serine, and tryptophan; Cambridge Isotope Laboratories). Homogenates were rotated for 1 hour at -20°C. Homogenates were centrifuged for 10 minutes at 21,000 x g, and 150 µl of the cleared metabolite extracts were transferred to autosampler vials and dried using a SpeedVac vacuum concentrator (Thermo). Dried metabolite extracts were reconstituted in 30 μl of 11.4 mg/ml methoxyamine (MOX) in anhydrous pyridine, vortexed for 5 minutes, and heated for 1 hour at 60°C. Next, to each sample 20 μl of N,O-Bis(trimethylsilyl)trifluoroacetamide (TMS) was added, samples were vortexed for 1 minute, and heated for 30 minutes at 60°C.","SAMPLEPREP_PROTOCOL_FILENAME":"GC_Method_Tissue.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"1 μl of derivatized sample was injected into a Trace 1300 GC (Thermo) fitted with a TraceGold TG-5SilMS column (Thermo) operating under the following conditions: split ratio = 20-1, split flow = 24 μl/minute, purge flow = 5 ml/minute, carrier mode = Constant Flow, and carrier flow rate = 1.2 ml/minute. The GC oven temperature gradient was as follows: 80°C for 3 minutes, increasing at a rate of 20°C/minute to 280°C, and holding at a temperature at 280°C for 8 minutes.","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Thermo Trace 1310","COLUMN_NAME":"Thermo TraceGold TG-5SilMS column","METHODS_FILENAME":"GC_Method_Tissue.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo ISQ","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"The MS was operated from 3.90 to 21.00 minutes in EI mode (-70eV) using select ion monitoring (SIM). Data were QC normalized using NOREVA (PMID: 28525573)."},

"MS_METABOLITE_DATA":{
"Units":"AU",

"Data":[{"Metabolite":"1-Octadecanol","S1":"26.64610733","S2":"25.7712191","S3":"19.0643173","S4":"22.9165523","S5":"25.83906135","S6":"25.55278951","S7":"23.08564904","S8":"20.4967554","S9":"27.53469924"},{"Metabolite":"2-Hydroxybutyrate","S1":"22.80155364","S2":"20.92778131","S3":"21.53421694","S4":"21.80288908","S5":"25.42567553","S6":"25.8108873","S7":"21.6375929","S8":"20.4232139","S9":"25.08876288"},{"Metabolite":"2-Hydroxyglutarate","S1":"45.41856423","S2":"36.94068451","S3":"19.46434509","S4":"18.11133929","S5":"17.31244327","S6":"25.57615586","S7":"18.11993997","S8":"21.63660465","S9":"26.15451947"},{"Metabolite":"3-Phosphoglycerate","S1":"31.4036057","S2":"21.51736916","S3":"17.15902217","S4":"17.01026064","S5":"27.33415596","S6":"25.85844134","S7":"18.93702629","S8":"35.96868861","S9":"20.90611314"},{"Metabolite":"6-Phosphogluconate","S1":"21.08312625","S2":"20.06891181","S3":"24.22549457","S4":"19.68601324","S5":"27.46542188","S6":"23.97424549","S7":"33.53406012","S8":"24.93381028","S9":"32.60621498"},{"Metabolite":"Aconitate","S1":"20.49884981","S2":"22.60888047","S3":"19.48431841","S4":"17.44020629","S5":"29.8780027","S6":"21.54298487","S7":"36.10743917","S8":"25.81104705","S9":"40.69280174"},{"Metabolite":"Adenine","S1":"26.30674338","S2":"26.18796058","S3":"24.39951207","S4":"24.6210554","S5":"26.59921414","S6":"25.53383074","S7":"25.71678708","S8":"21.97533971","S9":"24.05775137"},{"Metabolite":"Adonitol","S1":"27.37498647","S2":"41.98805816","S3":"40.41894819","S4":"33.70043083","S5":"31.72169679","S6":"46.7178548","S7":"49.83328799","S8":"44.17085753","S9":"30.52122133"},{"Metabolite":"Alanine","S1":"23.12639032","S2":"22.59944116","S3":"29.67834383","S4":"27.58780754","S5":"24.76943709","S6":"21.48796002","S7":"22.3730064","S8":"25.3280182","S9":"27.90855"},{"Metabolite":"alpha-Keto-beta-Methylvalerate (KMV)","S1":"26.29784792","S2":"20.72215374","S3":"28.93627311","S4":"33.13093688","S5":"12.62063716","S6":"23.77574464","S7":"12.51214235","S8":"21.25867553","S9":"21.39853287"},{"Metabolite":"alpha-Ketoglutarate","S1":"34.63761447","S2":"25.87622241","S3":"25.4676943","S4":"21.18343712","S5":"18.11666068","S6":"19.85276962","S7":"26.53668264","S8":"18.26076134","S9":"39.84199748"},{"Metabolite":"alpha-Ketoisocaproate (KIC)","S1":"18.74805329","S2":"20.74587268","S3":"26.43132506","S4":"27.33851116","S5":"26.35944445","S6":"30.10344146","S7":"28.78755903","S8":"20.10298036","S9":"29.01448088"},{"Metabolite":"alpha-Ketoisovalerate (KIV)","S1":"22.33266465","S2":"21.3643545","S3":"22.95686331","S4":"23.77404489","S5":"23.78823017","S6":"22.99649722","S7":"21.08373288","S8":"20.84791514","S9":"22.87596743"},{"Metabolite":"Aminoadipate","S1":"27.11466694","S2":"17.980257","S3":"31.46741104","S4":"20.7380752","S5":"14.99646961","S6":"30.7643353","S7":"19.11339816","S8":"26.82410626","S9":"18.11378038"},{"Metabolite":"Arachidic acid (Eicosanoic acid)","S1":"27.66032524","S2":"23.96039568","S3":"26.09655203","S4":"29.70213933","S5":"33.83729408","S6":"22.43164782","S7":"22.88513129","S8":"22.55900677","S9":"16.78219329"},{"Metabolite":"Arachidonate","S1":"28.01187687","S2":"25.1149575","S3":"21.92260908","S4":"24.86922352","S5":"24.18142261","S6":"25.47795471","S7":"27.57882908","S8":"22.41401491","S9":"28.46913227"},{"Metabolite":"Asparagine","S1":"24.81789952","S2":"26.89479811","S3":"22.18434838","S4":"27.39185237","S5":"19.7678155","S6":"32.18588714","S7":"25.06608938","S8":"25.25821763","S9":"29.42591118"},{"Metabolite":"Aspartate","S1":"18.97876758","S2":"20.92567359","S3":"34.17541437","S4":"21.09093231","S5":"21.62677","S6":"24.65142675","S7":"16.67504888","S8":"25.93894684","S9":"29.22648857"},{"Metabolite":"beta-Alanine","S1":"18.61776893","S2":"29.57355308","S3":"28.11967041","S4":"26.99188403","S5":"30.41453131","S6":"30.06530669","S7":"27.46883834","S8":"17.12136982","S9":"25.21992318"},{"Metabolite":"beta-Hydroxy beta-Methylbutyric acid (HMB)","S1":"17.74452714","S2":"32.53416692","S3":"26.55531103","S4":"23.48660801","S5":"23.00326989","S6":"29.37112795","S7":"29.98598663","S8":"23.32254844","S9":"36.50960365"},{"Metabolite":"beta-Hydroxybutyrate (3-Hydroxybutyrate)","S1":"23.80490849","S2":"31.06351754","S3":"36.25246623","S4":"24.45011913","S5":"13.60272247","S6":"29.63902104","S7":"36.03911419","S8":"15.46841824","S9":"25.364213"},{"Metabolite":"Cholesterol","S1":"25.86391366","S2":"24.35081476","S3":"21.67649199","S4":"22.60487048","S5":"24.55320203","S6":"25.29510717","S7":"28.2047009","S8":"25.47082686","S9":"27.77165552"},{"Metabolite":"Citraconate","S1":"34.96780574","S2":"26.23008084","S3":"22.04068082","S4":"27.64259181","S5":"20.83859329","S6":"15.85666998","S7":"19.79676204","S8":"39.34077849","S9":"18.81098123"},{"Metabolite":"Citrate","S1":"36.88853338","S2":"23.96561856","S3":"21.6912037","S4":"21.40774942","S5":"18.81608738","S6":"25.80958425","S7":"24.62053473","S8":"22.78065004","S9":"30.72873542"},{"Metabolite":"Citrulline","S1":"50.73069968","S2":"35.44564686","S3":"24.75348743","S4":"36.26321154","S5":"28.59612014","S6":"23.81155745","S7":"29.35138578","S8":"46.62844802","S9":"26.25841812"},{"Metabolite":"Cysteine","S1":"26.65020808","S2":"27.15020303","S3":"24.15224792","S4":"26.1008083","S5":"24.4878078","S6":"28.89527458","S7":"30.01352928","S8":"25.63594988","S9":"23.28731057"},{"Metabolite":"Dihydroxyacetone phosphate (DHAP)","S1":"28.44262564","S2":"18.58162936","S3":"13.98248172","S4":"14.43865214","S5":"21.70297378","S6":"18.41212532","S7":"17.33488764","S8":"37.78339766","S9":"16.67030089"},{"Metabolite":"Fructose","S1":"31.08437981","S2":"26.49474304","S3":"22.45030261","S4":"19.85620266","S5":"27.0657862","S6":"43.84573609","S7":"32.82063406","S8":"38.60551882","S9":"21.71946722"},{"Metabolite":"Fructose 6-phosphate","S1":"21.31536742","S2":"15.47041803","S3":"14.39538765","S4":"10.36889263","S5":"46.40091014","S6":"54.32045051","S7":"22.59392566","S8":"28.43348957","S9":"20.73206601"},{"Metabolite":"Fumarate","S1":"31.01180848","S2":"21.96485321","S3":"28.27772746","S4":"22.7496045","S5":"23.44645297","S6":"26.82398439","S7":"19.64286924","S8":"29.64750447","S9":"28.93383266"},{"Metabolite":"Gamma-aminobutyric acid (GABA)","S1":"28.26858928","S2":"31.71450161","S3":"16.08006947","S4":"22.18042071","S5":"23.43012076","S6":"28.21907101","S7":"29.37169191","S8":"22.68694197","S9":"31.04771242"},{"Metabolite":"Glucose","S1":"33.09235304","S2":"16.41976794","S3":"30.45747877","S4":"22.5578656","S5":"26.66438326","S6":"20.65184558","S7":"20.37426907","S8":"39.7055633","S9":"24.68674984"},{"Metabolite":"Glucose 6-phosphate","S1":"20.79267111","S2":"14.91608096","S3":"13.34588099","S4":"10.86397375","S5":"43.43498181","S6":"57.27790911","S7":"24.59566313","S8":"29.39809477","S9":"21.96213707"},{"Metabolite":"Glutamate","S1":"30.51043451","S2":"26.02090382","S3":"30.24535546","S4":"22.24278043","S5":"13.99729553","S6":"28.48774773","S7":"23.40302303","S8":"26.009802","S9":"19.80321874"},{"Metabolite":"Glutamine","S1":"24.17921932","S2":"30.12063264","S3":"29.25597434","S4":"24.69104563","S5":"19.08289988","S6":"26.61340095","S7":"27.88583851","S8":"22.8653454","S9":"22.85641989"},{"Metabolite":"Glycerate","S1":"32.08027207","S2":"22.92748752","S3":"22.71360206","S4":"22.31100741","S5":"30.18258116","S6":"28.55416436","S7":"23.42934157","S8":"29.53657981","S9":"22.52910055"},{"Metabolite":"Glycine","S1":"20.03961223","S2":"28.5877929","S3":"22.26324774","S4":"26.13603896","S5":"26.4614104","S6":"27.02252208","S7":"32.21804413","S8":"19.05899065","S9":"28.19793209"},{"Metabolite":"Guanine","S1":"25.51722298","S2":"20.39339419","S3":"23.90646419","S4":"23.65890613","S5":"44.26277603","S6":"23.27917246","S7":"26.43869221","S8":"19.32543641","S9":"27.05584124"},{"Metabolite":"Heneicosylic acid (Heneicosanoic acid)","S1":"23.78250458","S2":"41.72489849","S3":"22.27593046","S4":"38.29674707","S5":"30.289756","S6":"22.28818658","S7":"18.77551491","S8":"17.34081243","S9":"18.17661225"},{"Metabolite":"Heptadecanoic acid","S1":"25.74340358","S2":"26.57313874","S3":"23.58045812","S4":"23.76706929","S5":"28.26254118","S6":"25.7187051","S7":"27.8110126","S8":"20.38580908","S9":"29.7778673"},{"Metabolite":"Histamine","S1":"22.29059526","S2":"20.76147408","S3":"23.68671846","S4":"22.19244791","S5":"25.02061115","S6":"33.31188775","S7":"17.24027622","S8":"20.17272136","S9":"24.79823924"},{"Metabolite":"Histidine","S1":"23.87267354","S2":"19.46369616","S3":"25.76054693","S4":"26.3470725","S5":"18.74105395","S6":"33.84761456","S7":"18.86853878","S8":"16.49842796","S9":"19.73545657"},{"Metabolite":"Homoserine","S1":"31.80938393","S2":"24.01034207","S3":"23.10905688","S4":"25.45356225","S5":"18.50758903","S6":"23.80447684","S7":"20.64824615","S8":"25.37701874","S9":"21.0511916"},{"Metabolite":"Hypotaurine","S1":"29.72942888","S2":"20.5455655","S3":"15.09883522","S4":"21.38413381","S5":"18.38503995","S6":"26.09150502","S7":"21.60668406","S8":"23.86171032","S9":"40.53562338"},{"Metabolite":"Hypoxanthine","S1":"24.45271271","S2":"24.45226248","S3":"26.26895162","S4":"24.49381211","S5":"27.50670641","S6":"29.57091278","S7":"26.89349347","S8":"24.36006369","S9":"25.65478517"},{"Metabolite":"Inosine","S1":"20.37188012","S2":"26.69987771","S3":"26.54061981","S4":"25.61800849","S5":"26.42653806","S6":"27.31136636","S7":"30.86208172","S8":"22.77598927","S9":"29.72169823"},{"Metabolite":"Inotisol","S1":"28.03025681","S2":"17.87461987","S3":"24.48842274","S4":"20.93712196","S5":"20.73011568","S6":"27.94033734","S7":"27.90776565","S8":"30.49917065","S9":"21.89416399"},{"Metabolite":"Isocitrate","S1":"33.83476328","S2":"22.6987647","S3":"22.75462234","S4":"19.0160822","S5":"20.42338287","S6":"23.7398812","S7":"24.47911747","S8":"25.53789099","S9":"30.36426737"},{"Metabolite":"Isoleucine","S1":"18.17678587","S2":"28.71460295","S3":"33.11731594","S4":"27.50906625","S5":"18.12437881","S6":"30.28606622","S7":"31.08587506","S8":"22.08515063","S9":"20.3645636"},{"Metabolite":"Lactate","S1":"26.97353075","S2":"14.51561278","S3":"25.23202233","S4":"16.73832397","S5":"47.18889873","S6":"38.35244015","S7":"14.10846614","S8":"26.84813241","S9":"24.65487039"},{"Metabolite":"Lauric acid","S1":"32.67489035","S2":"32.28731371","S3":"26.63356096","S4":"31.27883047","S5":"20.4844131","S6":"21.42575651","S7":"24.8554418","S8":"36.96220584","S9":"22.5826739"},{"Metabolite":"Leucine","S1":"16.54622802","S2":"25.61244629","S3":"32.76641614","S4":"26.86105703","S5":"16.96632978","S6":"30.49009596","S7":"30.61389195","S8":"21.37994925","S9":"19.75809237"},{"Metabolite":"Linoleate","S1":"33.02113185","S2":"26.15718849","S3":"25.65086578","S4":"23.19482666","S5":"25.6022056","S6":"26.98105869","S7":"28.21145609","S8":"29.39032064","S9":"26.98332495"},{"Metabolite":"Linolenic 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"Metabolites":[{"metabolite_name":"1-Octadecanol"},{"metabolite_name":"2-Hydroxybutyrate"},{"metabolite_name":"2-Hydroxyglutarate"},{"metabolite_name":"3-Phosphoglycerate"},{"metabolite_name":"6-Phosphogluconate"},{"metabolite_name":"Aconitate"},{"metabolite_name":"Adenine"},{"metabolite_name":"Adonitol"},{"metabolite_name":"Alanine"},{"metabolite_name":"alpha-Keto-beta-Methylvalerate (KMV)"},{"metabolite_name":"alpha-Ketoglutarate"},{"metabolite_name":"alpha-Ketoisocaproate (KIC)"},{"metabolite_name":"alpha-Ketoisovalerate (KIV)"},{"metabolite_name":"Aminoadipate"},{"metabolite_name":"Arachidic acid (Eicosanoic acid)"},{"metabolite_name":"Arachidonate"},{"metabolite_name":"Asparagine"},{"metabolite_name":"Aspartate"},{"metabolite_name":"beta-Alanine"},{"metabolite_name":"beta-Hydroxy beta-Methylbutyric acid (HMB)"},{"metabolite_name":"beta-Hydroxybutyrate (3-Hydroxybutyrate)"},{"metabolite_name":"Cholesterol"},{"metabolite_name":"Citraconate"},{"metabolite_name":"Citrate"},{"metabolite_name":"Citrulline"},{"metabolite_name":"Cysteine"},{"metabolite_name":"Dihydroxyacetone phosphate (DHAP)"},{"metabolite_name":"Fructose"},{"metabolite_name":"Fructose 6-phosphate"},{"metabolite_name":"Fumarate"},{"metabolite_name":"Gamma-aminobutyric acid (GABA)"},{"metabolite_name":"Glucose"},{"metabolite_name":"Glucose 6-phosphate"},{"metabolite_name":"Glutamate"},{"metabolite_name":"Glutamine"},{"metabolite_name":"Glycerate"},{"metabolite_name":"Glycine"},{"metabolite_name":"Guanine"},{"metabolite_name":"Heneicosylic acid (Heneicosanoic acid)"},{"metabolite_name":"Heptadecanoic acid"},{"metabolite_name":"Histamine"},{"metabolite_name":"Histidine"},{"metabolite_name":"Homoserine"},{"metabolite_name":"Hypotaurine"},{"metabolite_name":"Hypoxanthine"},{"metabolite_name":"Inosine"},{"metabolite_name":"Inotisol"},{"metabolite_name":"Isocitrate"},{"metabolite_name":"Isoleucine"},{"metabolite_name":"Lactate"},{"metabolite_name":"Lauric acid"},{"metabolite_name":"Leucine"},{"metabolite_name":"Linoleate"},{"metabolite_name":"Linolenic acid"},{"metabolite_name":"Lysine"},{"metabolite_name":"Malate"},{"metabolite_name":"Mannose"},{"metabolite_name":"Methionine"},{"metabolite_name":"Myristic acid"},{"metabolite_name":"N-Acetylglycine"},{"metabolite_name":"N-Acetylmethionine"},{"metabolite_name":"N-Acetylserine"},{"metabolite_name":"Oleic acid"},{"metabolite_name":"O-Phosphoethanolamine"},{"metabolite_name":"O-Phosphoserine"},{"metabolite_name":"Ornithine"},{"metabolite_name":"Orotate"},{"metabolite_name":"Palmitate"},{"metabolite_name":"Phenylalanine"},{"metabolite_name":"Phosphoenolpyruvate"},{"metabolite_name":"Proline"},{"metabolite_name":"Pterin"},{"metabolite_name":"Pyruvate"},{"metabolite_name":"Ribose"},{"metabolite_name":"Ribose 5-phosphate"},{"metabolite_name":"Sedoheptulose 7-phosphate"},{"metabolite_name":"Serine"},{"metabolite_name":"Sphingosine"},{"metabolite_name":"Stearate"},{"metabolite_name":"Succinate"},{"metabolite_name":"Taurine"},{"metabolite_name":"Threonine"},{"metabolite_name":"Thymidine"},{"metabolite_name":"Thymine"},{"metabolite_name":"Tryptophan"},{"metabolite_name":"Tyrosine"},{"metabolite_name":"Uracil"},{"metabolite_name":"Urea"},{"metabolite_name":"Valine"},{"metabolite_name":"Xanthine"},{"metabolite_name":"Xylose"}]
}

}