{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002266","ANALYSIS_ID":"AN003703","VERSION":"1","CREATED_ON":"09-22-2022"},

"PROJECT":{"PROJECT_TITLE":"Kīlauea lava fuels phytoplankton bloom in the North Pacific Ocean - study of particulate metabolites","PROJECT_TYPE":"Marine Metabolomics","PROJECT_SUMMARY":"From June to August 2018, the eruption of the Kīlauea volcano on the island of Hawai‘i injected millions of cubic meters of molten lava into the nutrient-poor waters of the North Pacific Subtropical Gyre. The lava-impacted seawater was characterized by high concentrations of metals and nutrients that stimulated phytoplankton growth, resulting in an extensive plume of chlorophyll a that was detectable by satellite. Samples for particulate metabolites were collected from different stations surrounding the lava flowing into the ocean to see how marine microorganisms respond to exogenous inputs of nutrients and metals.","INSTITUTE":"University of Washington","DEPARTMENT":"School of Oceanography","LABORATORY":"Ingalls Lab","LAST_NAME":"Lionheart","FIRST_NAME":"Regina","ADDRESS":"1400 NE Campus Parkway, Seattle, Washington, 98195, USA","EMAIL":"regina16@uw.edu","PHONE":"2062216750","FUNDING_SOURCE":"Simons Foundation","PUBLICATIONS":"Wilson et al., Science September 2019","DOI":"http://dx.doi.org/10.21228/M8JQ4M"},

"STUDY":{"STUDY_TITLE":"Kīlauea lava fuels phytoplankton bloom in the North Pacific Ocean - study of particulate metabolites","STUDY_TYPE":"Study of particulate metabolites in phytoplankton blooms.","STUDY_SUMMARY":"These data are relative concentrations of targeted metabolites measured in particulate matter collected from near the Island of Hawai’i in July 2018 during the Kilauea eruption. Six stations were sampled, with station 2 representing the most geothermally impacted station closest to the lava entry. Station 6 was the most oligotrophic station. The particulate metabolites show evidence of altered community composition and metabolism at the geothermally impacted station. This station was within a phytoplankton bloom. The bloom was stimulated by lava heating deep seawater and driving upwelling, which then provided nutrients for diatom growth. See Wilson and Hawco, et. al. 2019 (DOI: 10.1126/science.aax4767) for a complete description of sample collection, phytoplankton bloom dynamics, and chemical modifications of seawater due to the eruption. Metabolites with high concentration (relative abundance per L of seawater) in the geothermally impacted station (St 2) compared to the other stations were: cytosine, hydroxyectoine, adenine, adenosine, thymine, glutamic acid, ectoine, deoxyadenosine, UDP-glucosamine, and guanosine. After normalizing to the particulate carbon concentration at each station, guanosine, glutamic acid, hydroxyectoine, ectoine, adenosine, deoxyadenosine, and UDP-glucosamine were enriched in the geothermally impacted station relative to other stations. Ectoine was the metabolite with the largest change between St 2 and St 6, regardless of normalization to L of seawater or to moles of particulate carbon, with dramatically higher concentrations in the geothermally impacted waters. Except for glutamic and proline, particulate amino acids were generally in higher concentrations in the oligotrophic station.","INSTITUTE":"University of Washington","DEPARTMENT":"School of Oceanography","LABORATORY":"Ingalls Lab","LAST_NAME":"Lionheart","FIRST_NAME":"Regina","ADDRESS":"1400 NE Campus Parkway, Seattle, Washington, 98195, USA","EMAIL":"regina16@uw.edu","PHONE":"2062216750","SUBMIT_DATE":"2022-08-10"},

"SUBJECT":{"SUBJECT_TYPE":"Water sample"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Smp_S2C2D5_A",
"Factors":{"Sampling_Date":"7/13/2018","Latitude":"19.415","Longitude":"154.8416667","Station":"S2","Cast":"C2","Depth":"5","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S2C2D5_A;181004_Smp_S2C2D5_A;181008_Smp_S2C2D5_A","Notes":"Sampled filtrate (1L)"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S2C2D5_B",
"Factors":{"Sampling_Date":"7/13/2018","Latitude":"19.415","Longitude":"154.8416667","Station":"S2","Cast":"C2","Depth":"5","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S2C2D5_B;181004_Smp_S2C2D5_B;181008_Smp_S2C2D5_B","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S2C2D5_C",
"Factors":{"Sampling_Date":"7/13/2018","Latitude":"19.415","Longitude":"154.8416667","Station":"S2","Cast":"C2","Depth":"5","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S2C2D5_C;181004_Smp_S2C2D5_C;181008_Smp_S2C2D5_C","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S5C1D5_A",
"Factors":{"Sampling_Date":"7/14/2018","Latitude":"19.09666667","Longitude":"154.505","Station":"S5","Cast":"C1","Depth":"5","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S5C1D5_A;181004_Smp_S5C1D5_A;181008_Smp_S5C1D5_A","Notes":"Sampled filtrate (1L)"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S5C1D5_B",
"Factors":{"Sampling_Date":"7/14/2018","Latitude":"19.09666667","Longitude":"154.505","Station":"S5","Cast":"C1","Depth":"5","sample_vol_filtered_L":"9"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S5C1D5_B;181004_Smp_S5C1D5_B;181008_Smp_S5C1D5_B","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S4C2D5_A",
"Factors":{"Sampling_Date":"7/14/2018","Latitude":"19.38166667","Longitude":"154.6766667","Station":"S4","Cast":"C2","Depth":"5","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S4C2D5_A;181004_Smp_S4C2D5_A;181008_Smp_S4C2D5_A","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S4C2D5_B",
"Factors":{"Sampling_Date":"7/14/2018","Latitude":"19.38166667","Longitude":"154.6766667","Station":"S4","Cast":"C2","Depth":"5","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S4C2D5_B;181004_Smp_S4C2D5_B;181008_Smp_S4C2D5_B","Notes":"Sampled filtrate (1L)"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S4C2D5_C",
"Factors":{"Sampling_Date":"7/14/2018","Latitude":"19.38166667","Longitude":"154.6766667","Station":"S4","Cast":"C2","Depth":"5","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S4C2D5_C;181004_Smp_S4C2D5_C;181008_Smp_S4C2D5_C","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S3TF_A",
"Factors":{"Sampling_Date":"7/14/2018","Latitude":"19.435","Longitude":"154.7933333","Station":"S3","Cast":"TF","Depth":"1","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S3TF_A;181004_Smp_S3TF_A;181008_Smp_S3TF_A","Notes":"from tow fish"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S6C3D5_A",
"Factors":{"Sampling_Date":"7/15/2018","Latitude":"18.695","Longitude":"154.5233333","Station":"S6","Cast":"C3","Depth":"5","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S6C3D5_A;181004_Smp_S6C3D5_A;181008_Smp_S6C3D5_A","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S6C3D5_B",
"Factors":{"Sampling_Date":"7/15/2018","Latitude":"18.695","Longitude":"154.5233333","Station":"S6","Cast":"C3","Depth":"5","sample_vol_filtered_L":"10"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S6C3D5_B;181004_Smp_S6C3D5_B;181008_Smp_S6C3D5_B","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S6C3D5_C",
"Factors":{"Sampling_Date":"7/15/2018","Latitude":"18.695","Longitude":"154.5233333","Station":"S6","Cast":"C3","Depth":"5","sample_vol_filtered_L":"9"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S6C3D5_C;181004_Smp_S6C3D5_C;181008_Smp_S6C3D5_C","Notes":"Sampled filtrate (1L)"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_HOT-LAVA-QC_1",
"Factors":{"Sampling_Date":"7/15/2018","Latitude":"18.74166667","Longitude":"155.3816667","Station":"QC","Cast":"Underway from ship","Depth":"5","sample_vol_filtered_L":"20"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Poo_HOT-LAVA-QC_1;181004_Poo_HOT-LAVA-QC_1;181008_Poo_HOT-LAVA-QC_1","Notes":"collected at 18 44.50 155 22.909 from ship underway uncontaminated seawater"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_HOT-LAVA-QC_2",
"Factors":{"Sampling_Date":"7/15/2018","Latitude":"18.74166667","Longitude":"155.3816667","Station":"QC","Cast":"Underway from ship","Depth":"5","sample_vol_filtered_L":"20"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Poo_HOT-LAVA-QC_2;181004_Poo_HOT-LAVA-QC_2;181008_Poo_HOT-LAVA-QC_2","Notes":"collected at 18 44.50 155 22.909 from ship underway uncontaminated seawater"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_HOT-LAVA-QC_3",
"Factors":{"Sampling_Date":"7/15/2018","Latitude":"18.74166667","Longitude":"155.3816667","Station":"QC","Cast":"Underway from ship","Depth":"5","sample_vol_filtered_L":"20"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Poo_HOT-LAVA-QC_3;181004_Poo_HOT-LAVA-QC_3;181008_Poo_HOT-LAVA-QC_3","Notes":"collected at 18 44.50 155 22.909 from ship underway uncontaminated seawater"}
},
{
"Subject ID":"-",
"Sample ID":"Blk_MQBlk_1",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Blk_MQBlk_1;181004_Blk_MQBlk_1;181008_Blk_MQBlk_1","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_TruePoo_DDApos20",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181008_Poo_TruePoo_DDApos20","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_TruePoo_DDApos35",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181008_Poo_TruePoo_DDApos35","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_TruePoo_DDApos50",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181008_Poo_TruePoo_DDApos50","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_TruePoo_Full1",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Poo_TruePoo_Full1;181004_Poo_TruePoo_Full1;181008_Poo_TruePoo_Full1","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_TruePoo_Full2",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Poo_TruePoo_Full2;181004_Poo_TruePoo_Full2;181008_Poo_TruePoo_Full2","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_TruePoo_Full3",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Poo_TruePoo_Full3;181004_Poo_TruePoo_Full3;181008_Poo_TruePoo_Full3","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_TruePoo_Half1",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Poo_TruePoo_Half1;181004_Poo_TruePoo_Half1;181008_Poo_TruePoo_Half1","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_TruePoo_Half2",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Poo_TruePoo_Half2;181004_Poo_TruePoo_Half2;181008_Poo_TruePoo_Half2","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Poo_TruePoo_Half3",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Poo_TruePoo_Half3;181004_Poo_TruePoo_Half3;181008_Poo_TruePoo_Half3","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_KM1513Poo7-26_1",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_KM1513Poo7-26_1;181004_Smp_KM1513Poo7-26_1;181008_Smp_KM1513Poo7-26_1","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S515mMS_A",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S515mMS_A;181004_Smp_S515mMS_A;181008_Smp_S515mMS_A","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S515mMS_B",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S515mMS_B;181004_Smp_S515mMS_B;181008_Smp_S515mMS_B","Notes":"NA"}
},
{
"Subject ID":"-",
"Sample ID":"Smp_S515mMS_C",
"Factors":{"Sampling_Date":"NA","Latitude":"NA","Longitude":"NA","Station":"NA","Cast":"NA","Depth":"NA","sample_vol_filtered_L":"NA"},
"Additional sample data":{"RAW_FILE_NAME":"181004_Smp_S515mMS_C;181004_Smp_S515mMS_C;181008_Smp_S515mMS_C","Notes":"NA"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Samples for particulate metabolites were collected from 5 different stations surrounding the lava flowing into the ocean off the island of Hawaii, all from a depth of 5m. At each sampling location, single, duplicate, or triplicate filters were collected using either niskins attached to a conductivity, temperature, depth array (CTD) or the underway intake. Samples (10 L) were collected into polycarbonate carboys, filtered onto 147 mm 0.2 μm PTFE filters using peristaltic pumps, polycarbonate filter holders, and Masterflex PharMed BPT tubing (Cole-Parmer). Filters were flash frozen in liquid nitrogen and stored at -80°C until extraction. In addition to our samples, we filtered MilliQ water through a 0.2 μm PTFE filter and extracted this filter alongside samples as a methodological blank.","SAMPLE_TYPE":"Phytoplankton","COLLECTION_METHOD":"CTD Niskin arrays","COLLECTION_LOCATION":"Hawai'i","VOLUMEORAMOUNT_COLLECTED":"10L","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"Samples (10 L) were collected into polycarbonate carboys, filtered onto 147 mm 0.2 μm PTFE filters using peristaltic pumps, polycarbonate filter holders, and Masterflex PharMed BPT tubing (Cole-Parmer). Filters were flash frozen in liquid nitrogen and stored at -80°C until extraction."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Each sample was extracted using a modified Bligh-Dyer extraction. Briefly, filters were cut up and put into 15 mL teflon centrifuge tubes containing a mixture of 100 µm and 400 µm silica beads. Heavy isotope-labeled internal standards were added along with ~2 mL of cold aqueous solvent (50:50 methanol:water) and ~3 mL of cold organic solvent (dichloromethane). The samples were shaken on a FastPrep-24 Homogenizer for 30 seconds and chilled in a -20 °C freezer repeatedly for three cycles of bead-beating and a total of 30 minutes of chilling. The organic and aqueous layers were separated by spinning samples in a centrifuge at 4,300 rpm for 2 minutes at 4 °C. The aqueous layer was removed to a new glass centrifuge tube. The remaining organic fraction was rinsed three more times with additions of 1 to 2 mL of 50:50 methanol:water. All aqueous rinses were combined for each sample and ~2 mL of cold dichloromethane was added to the combined aqueous layer. Tubes were shaken and centrifuged at 4,300 rpm for 2 minutes at 4°C. The aqueous layer was removed to a new glass vial and dried under N2 gas. The remaining organic layer in the bead beating tubes was transferred into the glass centrifuge tube and the bead beating tube was rinsed two more times with cold organic solvent. The combined organic rinses were centrifuged, transferred to a new glass vial, and dried under N2 gas. Dried aqueous fractions were re-dissolved in 380 µL of water. Dried organic fractions were re-dissolved in 400 µL of 90:10 methanol:toluene. 20 µL of isotope-labeled injection standards in water were added to the aqueous fractions. Blank filters were extracted alongside samples as methodological blanks."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"See attached summary","METHODS_FILENAME":"Ingalls_Metabolomics_LC_HOT-LAVA.txt","INSTRUMENT_NAME":"Q Exactive™ Plus Hybrid Quadrupole-Orbitrap","COLUMN_NAME":"Waters Acquity UPLC HSS Cyano (100 x 2.1mm, 1.8um)","COLUMN_TEMPERATURE":"35C","SOLVENT_A":"0.1% formic acid in water","SOLVENT_B":"0.1% formic acid in acetonitrile","CHROMATOGRAPHY_TYPE":"Reversed phase"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"Ingalls_Metabolomics_MS_HOT-LAVA.txt"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF hybrid Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","MS_COMMENTS":"See protocol","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"Normalized peak area",

"Data":[{"Metabolite":"Isobutyryl-L-carnitine","Smp_S2C2D5_A":"762217.0000","Smp_S2C2D5_B":"578166.0000","Smp_S2C2D5_C":"785311.0000","Smp_S5C1D5_A":"472831.0000","Smp_S5C1D5_B":"1129986.0000","Smp_S4C2D5_A":"508678.0000","Smp_S4C2D5_B":"633988.0000","Smp_S4C2D5_C":"972637.0000","Smp_S3TF_A":"436807.0000","Smp_S6C3D5_A":"421152.0000","Smp_S6C3D5_B":"223285.0000","Smp_S6C3D5_C":"245616.0000","Poo_HOT-LAVA-QC_1":"552651.0000","Poo_HOT-LAVA-QC_2":"557678.0000","Poo_HOT-LAVA-QC_3":"353764.0000","Blk_MQBlk_1":"85232.0000","Poo_TruePoo_DDApos20":"695432.0000","Poo_TruePoo_DDApos35":"667864.0000","Poo_TruePoo_DDApos50":"659695.0000","Poo_TruePoo_Full1":"655259.0000","Poo_TruePoo_Full2":"573653.0000","Poo_TruePoo_Full3":"600237.0000","Poo_TruePoo_Half1":"789478.0000","Poo_TruePoo_Half2":"653022.0000","Poo_TruePoo_Half3":"585690.0000","Smp_KM1513Poo7-26_1":"530228.0000","Smp_S515mMS_A":"230963.0000","Smp_S515mMS_B":"835536.0000","Smp_S515mMS_C":"411005.0000"},{"Metabolite":"Methylthioadenosine","Smp_S2C2D5_A":"194800.0000","Smp_S2C2D5_B":"327977.0000","Smp_S2C2D5_C":"305660.0000","Smp_S5C1D5_A":"265746.0000","Smp_S5C1D5_B":"535196.0000","Smp_S4C2D5_A":"525247.0000","Smp_S4C2D5_B":"563228.0000","Smp_S4C2D5_C":"553418.0000","Smp_S3TF_A":"626750.0000","Smp_S6C3D5_A":"437629.0000","Smp_S6C3D5_B":"313823.0000","Smp_S6C3D5_C":"238369.0000","Poo_HOT-LAVA-QC_1":"1247073.0000","Poo_HOT-LAVA-QC_2":"807670.0000","Poo_HOT-LAVA-QC_3":"654617.0000","Blk_MQBlk_1":"5461.0000","Poo_TruePoo_DDApos20":"987020.0000","Poo_TruePoo_DDApos35":"939074.0000","Poo_TruePoo_DDApos50":"806721.0000","Poo_TruePoo_Full1":"1142143.0000","Poo_TruePoo_Full2":"709801.0000","Poo_TruePoo_Full3":"772232.0000","Poo_TruePoo_Half1":"1364408.0000","Poo_TruePoo_Half2":"859952.0000","Poo_TruePoo_Half3":"943240.0000","Smp_KM1513Poo7-26_1":"1592767.0000","Smp_S515mMS_A":"1037711.0000","Smp_S515mMS_B":"1665284.0000","Smp_S515mMS_C":"1277422.0000"},{"Metabolite":"Phenylalanine","Smp_S2C2D5_A":"8904634.4110","Smp_S2C2D5_B":"8591266.4480","Smp_S2C2D5_C":"13620837.7800","Smp_S5C1D5_A":"10805589.3000","Smp_S5C1D5_B":"20702841.3900","Smp_S4C2D5_A":"11052629.1000","Smp_S4C2D5_B":"16284846.0500","Smp_S4C2D5_C":"11323110.7300","Smp_S3TF_A":"13886477.6000","Smp_S6C3D5_A":"15059085.7300","Smp_S6C3D5_B":"7359819.1300","Smp_S6C3D5_C":"6956409.3430","Poo_HOT-LAVA-QC_1":"10825464.7200","Poo_HOT-LAVA-QC_2":"11634311.8600","Poo_HOT-LAVA-QC_3":"12255146.7600","Blk_MQBlk_1":"2258858.1550","Poo_TruePoo_DDApos20":"14109145.9600","Poo_TruePoo_DDApos35":"13433296.3300","Poo_TruePoo_DDApos50":"13529949.3000","Poo_TruePoo_Full1":"13683267.8000","Poo_TruePoo_Full2":"13888825.7500","Poo_TruePoo_Full3":"13666292.9700","Poo_TruePoo_Half1":"12905420.5700","Poo_TruePoo_Half2":"12864986.4600","Poo_TruePoo_Half3":"12908056.5400","Smp_KM1513Poo7-26_1":"25498350.2900","Smp_S515mMS_A":"7652359.8530","Smp_S515mMS_B":"18960872.9100","Smp_S515mMS_C":"10997814.2100"},{"Metabolite":"Propionyl-L-carnitine","Smp_S2C2D5_A":"1409331.0000","Smp_S2C2D5_B":"3772785.0000","Smp_S2C2D5_C":"2215618.0000","Smp_S5C1D5_A":"2514498.0000","Smp_S5C1D5_B":"2772100.0000","Smp_S4C2D5_A":"1565469.0000","Smp_S4C2D5_B":"1697152.0000","Smp_S4C2D5_C":"3930929.0000","Smp_S3TF_A":"1320704.0000","Smp_S6C3D5_A":"3102397.0000","Smp_S6C3D5_B":"1262666.0000","Smp_S6C3D5_C":"1141333.0000","Poo_HOT-LAVA-QC_1":"1343975.0000","Poo_HOT-LAVA-QC_2":"1391397.0000","Poo_HOT-LAVA-QC_3":"960291.0000","Blk_MQBlk_1":"43274.0000","Poo_TruePoo_DDApos20":"2373019.0000","Poo_TruePoo_DDApos35":"2252879.0000","Poo_TruePoo_DDApos50":"2360163.0000","Poo_TruePoo_Full1":"2281114.0000","Poo_TruePoo_Full2":"1916436.0000","Poo_TruePoo_Full3":"2010506.0000","Poo_TruePoo_Half1":"2246682.0000","Poo_TruePoo_Half2":"2234672.0000","Poo_TruePoo_Half3":"2121038.0000","Smp_KM1513Poo7-26_1":"1322250.0000","Smp_S515mMS_A":"577655.0000","Smp_S515mMS_B":"2092949.0000","Smp_S515mMS_C":"1155229.0000"},{"Metabolite":"Tyrosine","Smp_S2C2D5_A":"11522151.8700","Smp_S2C2D5_B":"10693045.1600","Smp_S2C2D5_C":"16452570.2600","Smp_S5C1D5_A":"9333986.0350","Smp_S5C1D5_B":"7507395.7380","Smp_S4C2D5_A":"6947626.2140","Smp_S4C2D5_B":"11169250.4900","Smp_S4C2D5_C":"17499193.0500","Smp_S3TF_A":"7141733.8300","Smp_S6C3D5_A":"5362752.3140","Smp_S6C3D5_B":"3909864.7150","Smp_S6C3D5_C":"4620251.1150","Poo_HOT-LAVA-QC_1":"8960743.1770","Poo_HOT-LAVA-QC_2":"10166664.5100","Poo_HOT-LAVA-QC_3":"10758253.5700","Blk_MQBlk_1":"819664.0594","Poo_TruePoo_DDApos20":"9350747.0300","Poo_TruePoo_DDApos35":"9330103.8320","Poo_TruePoo_DDApos50":"9440061.4500","Poo_TruePoo_Full1":"8919838.6580","Poo_TruePoo_Full2":"9299433.7460","Poo_TruePoo_Full3":"9608010.2280","Poo_TruePoo_Half1":"10067083.8500","Poo_TruePoo_Half2":"10820729.5600","Poo_TruePoo_Half3":"10820548.8800","Smp_KM1513Poo7-26_1":"70382660.3700","Smp_S515mMS_A":"6883391.6890","Smp_S515mMS_B":"9182920.4270","Smp_S515mMS_C":"8822762.2130"},{"Metabolite":"Vitamin B3","Smp_S2C2D5_A":"3029162.9200","Smp_S2C2D5_B":"3551500.4710","Smp_S2C2D5_C":"3641971.4400","Smp_S5C1D5_A":"","Smp_S5C1D5_B":"","Smp_S4C2D5_A":"3037229.0420","Smp_S4C2D5_B":"4159817.8240","Smp_S4C2D5_C":"4027403.7270","Smp_S3TF_A":"5313747.8550","Smp_S6C3D5_A":"4862686.4960","Smp_S6C3D5_B":"","Smp_S6C3D5_C":"","Poo_HOT-LAVA-QC_1":"4398078.6080","Poo_HOT-LAVA-QC_2":"6632604.1560","Poo_HOT-LAVA-QC_3":"6991003.3830","Blk_MQBlk_1":"866871.7006","Poo_TruePoo_DDApos20":"4594392.7310","Poo_TruePoo_DDApos35":"4758521.2870","Poo_TruePoo_DDApos50":"4709788.6840","Poo_TruePoo_Full1":"4634509.1240","Poo_TruePoo_Full2":"6535609.6640","Poo_TruePoo_Full3":"6392980.1180","Poo_TruePoo_Half1":"","Poo_TruePoo_Half2":"","Poo_TruePoo_Half3":"","Smp_KM1513Poo7-26_1":"156886137808.0000","Smp_S515mMS_A":"4285404.9980","Smp_S515mMS_B":"6458139.1180","Smp_S515mMS_C":"5389101.1490"}],

"Metabolites":[{"Metabolite":"Isobutyryl-L-carnitine","pubchem_id":"","inchi_key":"","kegg_id":"NA","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"232.154884","":""},{"Metabolite":"Methylthioadenosine","pubchem_id":"","inchi_key":"","kegg_id":"cpd:C00170","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"298.097387","":""},{"Metabolite":"Phenylalanine","pubchem_id":"","inchi_key":"","kegg_id":"cpd:C00079","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"166.086804","":""},{"Metabolite":"Propionyl-L-carnitine","pubchem_id":"","inchi_key":"","kegg_id":"cpd:C03017","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"218.139234","":""},{"Metabolite":"Tyrosine","pubchem_id":"","inchi_key":"","kegg_id":"cpd:C00082","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"182.081719","":""},{"Metabolite":"Vitamin B3","pubchem_id":"938","inchi_key":"","kegg_id":"cpd:C00253","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"124.039854","":""}]
}

}