{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002330","ANALYSIS_ID":"AN003801","VERSION":"1","CREATED_ON":"November 2, 2022, 8:12 am"},

"PROJECT":{"PROJECT_TITLE":"Early-stage responses to Plasmodiophora brassicae at the metabolome levels in clubroot resistant and susceptible oilseed Brassica napus","PROJECT_TYPE":"MS quantitative analysis","PROJECT_SUMMARY":"Clubroot, a devastating soil-borne root disease, in Brassicaceae is caused by Plasmodiophora brassicae Woronin, an obligate biotrophic protist. Plant growth and development, as well as seed yield of Brassica crops, are severely affected due to this disease. Several reports described the molecular responses of B. napus to P. brassicae; however, information on the early stages of pathogenesis is limited. In this study, we have used metabolomics approach to characterize P. brassicae pathogenesis at 1-, 4-, and 7- days post-inoculation (DPI) in clubroot resistant (CR) and susceptible (CS) doubled-haploid (DH) lines. Several metabolites related to organic acids (e.g., citrate, pyruvate), amino acids (e.g., proline, aspartate), sugars, and mannitol, were differentially accumulated in roots in response to pathogen infection when the CR and CS genotypes were compared. Our results suggest important roles for these metabolites in mediating resistance to clubroot disease. To our knowledge, this is the first report of an integrated metabolome analysis aimed at characterizing the molecular basis of resistance to clubroot in canola.","INSTITUTE":"Trent University","DEPARTMENT":"Biology","LABORATORY":"Emery Lab","LAST_NAME":"Kisiala","FIRST_NAME":"Anna","ADDRESS":"1600 West Bank Drive, Trent University, Peterborough, ON, K9L 0G2, Canada","EMAIL":"annakisiala@trentu.ca","PHONE":"7057481011","FUNDING_SOURCE":"Alberta Agriculture and Forestry","CONTRIBUTORS":"Dinesh Adhikary, Anna Kisiala, Ananya Sarkar, Urmila Basu, Habibur Rahman, Neil Emery, Nat N. V. Kav"},

"STUDY":{"STUDY_TITLE":"Early-stage responses to Plasmodiophora brassicae at the metabolome levels in clubroot resistant and susceptible oilseed Brassica napus","STUDY_TYPE":"Timecourse experiment","STUDY_SUMMARY":"A total of 36 samples comprised two types of genotypes [CR (5 individuals pooled in each biological replicate) and CS (5 individuals pooled in each biological replicate)], two treatments (inoculated and uninoculated) and three biological replicates generated from three independent experiments and collected at 1-, 4-, and 7–DPI were used to extract primary and secondary metabolites and analyse the differences among the treatments.","INSTITUTE":"Trent University","DEPARTMENT":"Biology","LABORATORY":"Emery Lab","LAST_NAME":"Kisiala","FIRST_NAME":"Anna","ADDRESS":"1600 West Bank Drive, Trent University","EMAIL":"annakisiala@trentu.ca","PHONE":"7057481011"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Brassica napus","TAXONOMY_ID":"3708"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"C18-RN1-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN1-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN1-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN1-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN1-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN1-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN1-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN1-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN2-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN2-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN2-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN2-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN2-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN2-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN2-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN2-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN3-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN3-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN3-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN3-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN3-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN3-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN3-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN3-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN4-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN4-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN4-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN4-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN4-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN4-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN4-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN4-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN5-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN5-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN5-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN5-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN5-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN5-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN5-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN5-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN6-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN6-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN6-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN6-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN6-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN6-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN6-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN6-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN7-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN7-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN7-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN7-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN7-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN7-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN7-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN7-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN8-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN8-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN8-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN8-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN8-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN8-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN8-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN8-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN9-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN9-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN9-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN9-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN9-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN9-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN9-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN9-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN10-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN10-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN10-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN10-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN10-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN10-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN10-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN10-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN11-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN11-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN11-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN11-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN11-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN11-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN11-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN11-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN12-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN12-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN12-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN12-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN12-pos",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN12-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN12-neg",
"Factors":{"Developmental stage (DPI)":"day 1","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN12-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN13-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN13-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN13-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN13-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN13-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN13-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN13-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN13-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN14-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN14-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN14-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN14-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN14-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN14-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN14-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN14-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN15-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN15-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN15-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN15-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN15-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN15-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN15-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN15-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN16-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN16-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN16-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN16-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN16-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN16-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN16-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN16-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN17-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN17-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN17-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN17-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN17-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN17-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN17-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN17-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN18-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN18-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN18-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN18-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN18-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN18-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN18-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN18-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN19-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN19-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN19-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN19-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN19-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN19-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN19-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN19-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN20-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN20-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN20-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN20-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN20-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN20-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN20-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN20-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN21-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN21-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN21-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN21-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN21-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN21-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN21-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN21-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN22-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN22-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN22-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN22-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN22-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN22-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN22-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN22-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN23-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN23-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN23-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN23-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN23-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN23-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN23-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN23-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN24-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN24-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN24-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN24-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN24-pos",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN24-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN24-neg",
"Factors":{"Developmental stage (DPI)":"day 4","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN24-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN25-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN25-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN25-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN25-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN25-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN25-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN25-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN25-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN26-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN26-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN26-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN26-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN26-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN26-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN26-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN26-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN27-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN27-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN27-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN27-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN27-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN27-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN27-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN27-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN28-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN28-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN28-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN28-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN28-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN28-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN28-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN28-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN29-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN29-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN29-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN29-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN29-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN29-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN29-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN29-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN30-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN30-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN30-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN30-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN30-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN30-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN30-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Resistant","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN30-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN31-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN31-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN31-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN31-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN31-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN31-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN31-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN31-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN32-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN32-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN32-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN32-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN32-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN32-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN32-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN32-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN33-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN33-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN33-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN33-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN33-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN33-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN33-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN33-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN34-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN34-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN34-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN34-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN34-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN34-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN34-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN34-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN35-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN35-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN35-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN35-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN35-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN35-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN35-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN35-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN36-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN36-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"C18-RN36-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"C18-RN36-neg.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN36-pos",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN36-pos.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"HILIC-RN36-neg",
"Factors":{"Developmental stage (DPI)":"day 7","Genotype":"Susceptible","Treatment":"Treated with Plasmodiophora brassicae"},
"Additional sample data":{"RAW_FILE_NAME":"HILIC-RN36-neg.mzXML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"A total of 36 samples comprised two types of genotypes [CR (5 individuals pooled in each biological replicate) and CS (5 individuals pooled in each biological replicate)], two treatments (inoculated and uninoculated) and three biological replicates generated from three independent experiments and collected at 1-, 4-, and 7–DPI were used. Immediately after collection, all root samples were flash-frozen in liquid nitrogen and stored at -80 °C.","COLLECTION_PROTOCOL_FILENAME":"Collection_protocol-canola","SAMPLE_TYPE":"Plant","COLLECTION_LOCATION":"Alberta, Canada","STORAGE_CONDITIONS":"-80℃","COLLECTION_VIALS":"2 mL round-bottom Eppendorf tubes","STORAGE_VIALS":"2 mL round-bottom Eppendorf tubes"},

"TREATMENT":{"TREATMENT_SUMMARY":"A single spore-derived isolate of P. brassicae pathotype 3H (P3H) was used in this study. To assess the disease progression post-inoculation, histology, and scanning electron microscopy (SEM) was conducted on P. brassicae inoculated and uninoculated root tissues. Samples were collected by Adhikary as follows: seedlings were randomly selected, gently uprooted from the soil, the roots were washed thoroughly and collected in triplicates at after 24 hours of inoculation [hereinafter indicated as 1-day post-inoculation (DPI)], 4-, and 7-DPI."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Approximately 100 mg of fresh weight of root tissue samples (n=3) were aliquoted from finely homogenized frozen root tissues, placed into 2 mL safe-lock centrifuge tubes with two zirconium oxide beads, flash frozen in liquid nitrogen and stored at − 80 °C until further processing. To accommodate for different extraction procedures, two sample sets were prepared from the same bulk to independently analyze the content of primary metabolites (free amino acids, sugars and sugar phosphates, organic acids), and secondary metabolites (glucosinolates) using High Performance Liquid Chromatography – High-Resolution Accurate Mass – Full Scan Mass Spectrometry (HPLC-(HRAM)-MS). Samples for extraction of primary and secondary metabolites were spiked with 10ng of two labeled aromatic CKs (13C5-oT and 2H7-BAR) and extracted with ice-cold methanol (methanol-water [8:2, v/v]) following procedures of Chen et al 3 with modifications. Each filtered extract was split in half and each 500 µL transferred to a new 2 mL tube. Divided extracts were evaporated to dryness at ambient temperature in a speed vac concentrator. Sample residues designed for the analysis of primary metabolites were redissolved in 500 µl of 90% acetonitrile (acetonitrile: water, v/v) and samples for the analysis of glucosinolates were redissolved in 500 µL of 5% acetonitrile with 0.1% formic acid (acetonitrile: formic acid: water, v/v/v). Additionally, sample mixtures composed of 10uL of each sample extract were prepared separately for primary and secondary metabolite analysis and used to generate MS/MS data for compound identification. All samples were filtered using 0.2 µm PVDF spin filter with 2 mL receiver tubes (InnoSep Spin, Canadian Life Sciences, Peterborough, Canada) and transferred to insert-equipped 2 mL HPLC vials. A volume of 25 μL of each sample was injected into a Dionex UltiMate 3000 HPLC coupled to a QExactive Orbitrap mass spectrometer.","SAMPLEPREP_PROTOCOL_FILENAME":"Protocol_methods-canola","EXTRACTION_METHOD":"80% MeOH","EXTRACT_CLEANUP":"0.2 µm PVDF spin filter with 2 mL receiver tubes","EXTRACT_STORAGE":"-20℃","SAMPLE_RESUSPENSION":"Primary metabolites - 90% ACN, glucosinolates - 5% ACN","SAMPLE_SPIKING":"two labeled aromatic CKs (13C5-oT and 2H7-BAR)"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Primary metabolites were resolved with an InfinityLab Poroshell 120 HILIC-Z column (2.1 × 100 mm, 2.7 µm; Agilent, Santa Clara, CA, US), using a flow rate of 0.2 mL min-1 with a mobile phase of 10 mM ammonium bicarbonate in water (A) and 10 mM ammonium bicarbonate in 95% acetonitrile (B). The following gradient was used to elute the analytes: mobile phase 100% B decreased to 90% over 2.5 min and to 50% over the next 5 min and returned to 100% over 0.5 min for 12 min of column re-equilibration.","CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000 RS","COLUMN_NAME":"Agilent, InfinityLab Poroshell 120 HILIC-Z (2.1 × 100 mm, 2.7 µm)","FLOW_RATE":"0.2 ml/min","COLUMN_TEMPERATURE":"RT","SOLVENT_A":"10 mM ammonium bicarbonate in water","SOLVENT_B":"10 mM ammonium bicarbonate in 95% acetonitrile","INJECTION_TEMPERATURE":"4"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"WQC","OPERATOR_NAME":"Anna Kisiala"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"The Orbitrap QExactive was operated with a heated electrospray ionization (HESI) probe in positive mode. Temperatures of the HESI-II auxiliary gas heater and capillary were 450 and 300 °C, respectively, and the spray voltage was 3.9 kV. Sheath, auxiliary, and sweep gases were operated at 30, 8, and 0 (arbitrary units), respectively, and the S-lens RF level was 60. Each sample was analyzed using a mass range of m/z 70−900, and data were acquired at 70,000 resolution, automatic gain control (AGC) target of 1e6, and maximum injection time (IT) of 100 ms. Additionally, the top 10 data-dependent acquisition experiments were performed for the mixed sample from each group (HILIC and C18) to obtain compound MS/MS spectra. Processing of all full scan and ddMS2 data was conducted using the Xcalibur 4.1 software. Metabolites were identified by accurate mass, comparison of retention times to authentic standards or by accurate mass and comparison of fragmentation patterns to MS/MS databases (METLIN, PubChem). Metabolite relative concentration was normalized based on the average recoveries of 13C5-oT and 2H7-BAR as the internal standards"},

"MS_METABOLITE_DATA":{
"Units":"normalized relative level",

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"Metabolites":[{"Metabolite":"Alanine","quanitfied m/z":"90.05495","PubChemID":"5950","KEGG ID":"C00041"},{"Metabolite":"Asparagine","quanitfied m/z":"133.06076","PubChemID":"236","KEGG ID":"C00152"},{"Metabolite":"Aspartate","quanitfied m/z":"134.04478","PubChemID":"424","KEGG ID":"C16433"},{"Metabolite":"Betaine","quanitfied m/z":"118.08625","PubChemID":"247","KEGG ID":"C00719    "},{"Metabolite":"Citruline","quanitfied m/z":"176.10295","PubChemID":"9750","KEGG ID":"D07706"},{"Metabolite":"Glutamine","quanitfied m/z":"148.06043","PubChemID":"5961","KEGG ID":"C00303"},{"Metabolite":"Histidine","quanitfied m/z":"156.07674","PubChemID":"6274","KEGG ID":"D00032"},{"Metabolite":"Homoserine","quanitfied m/z":"120.06551","PubChemID":"779","KEGG ID":"C00263"},{"Metabolite":"Isoleucine","quanitfied m/z":"132.1019","PubChemID":"791","KEGG ID":"D00065"},{"Metabolite":"Kynurenine","quanitfied m/z":"209.09206","PubChemID":"161166","KEGG ID":"C00328  "},{"Metabolite":"L-Aminoadipate","quanitfied m/z":"162.07608","PubChemID":"6992111","KEGG ID":"C00956"},{"Metabolite":"Leucine","quanitfied m/z":"132.1019","PubChemID":"6106","KEGG ID":"D00030"},{"Metabolite":"Methionine","quanitfied m/z":"150.05832","PubChemID":"6147","KEGG ID":"C00073"},{"Metabolite":"Phenylalanine","quanitfied m/z":"166.08625","PubChemID":"6140","KEGG ID":"D00021  "},{"Metabolite":"Proline","quanitfied m/z":"116.0706","PubChemID":"145742","KEGG ID":" C00148"},{"Metabolite":"S-Adenosyl-L-homocysteine","quanitfied m/z":"385.12884","PubChemID":"439155","KEGG ID":"C00021"},{"Metabolite":"Sarcosine","quanitfied m/z":"90.05495","PubChemID":"1088","KEGG ID":"C00213"},{"Metabolite":"Serine","quanitfied m/z":"106.04986","PubChemID":"5951","KEGG ID":"C00065"},{"Metabolite":"Threonine","quanitfied m/z":"120.06551","PubChemID":"6288","KEGG ID":"D00041 "},{"Metabolite":"Tryptophan","quanitfied m/z":"205.09715","PubChemID":"6305","KEGG ID":"D00020"},{"Metabolite":"Tyrosine","quanitfied m/z":"182.08116","PubChemID":"6057","KEGG ID":"C00082"},{"Metabolite":"Valine","quanitfied m/z":"118.08625","PubChemID":"6287","KEGG ID":"D00039"}]
}

}