{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002361","ANALYSIS_ID":"AN003856","VERSION":"1","CREATED_ON":"06-06-2023"},

"PROJECT":{"PROJECT_TITLE":"UCP2-dependent redox-sensing in POMC neurons regulates feeding","PROJECT_SUMMARY":"Paradoxically, glucose, the primary driver of satiety, activates a small population of anorexigenic POMC neurons. Here we show that lactate levels in the circulation and in the cerebrospinal fluid are elevated in fed state and addition of lactate to glucose activates the majority of POMC neurons while increasing cytosolic NADH generation, mitochondrial respiration and extracellular pyruvate levels. Inhibition of lactate dehydrogenases diminishes mitochondrial respiration, NADH production, and POMC neuronal activity. However, inhibition of the mitochondrial pyruvate carrier has no effect. POMC-specific downregulation of Ucp2 (Ucp2PomcKO), a molecule regulated by fatty acid metabolism and shown to play a role as transporter in the malate-aspartate shuttle, abolishes lactate- and glucose-sensing of POMC neurons. Ucp2PomcKO mice have impaired glucose metabolism and are prone to obesity on a high fat diet. Altogether, our data show that lactate through redox signaling and blocking mitochondrial glucose utilization activates POMC neurons to regulate feeding and glucose metabolism.","INSTITUTE":"Columbia University","LAST_NAME":"Diano","FIRST_NAME":"Sabrina","ADDRESS":"1150 St. Nicholas Avenue Russ Berrie Medical Science Pavilion Rm 405 New York, NY, 10032","EMAIL":"sd3449@cumc.columbia.edu","PHONE":"212 8514554","DOI":"http://dx.doi.org/10.21228/M8R12T"},

"STUDY":{"STUDY_TITLE":"UCP2-dependent redox-sensing in POMC neurons regulates feeding","STUDY_SUMMARY":"Paradoxically, glucose, the primary driver of satiety, activates a small population of anorexigenic POMC neurons. Here we show that lactate levels in the circulation and in the cerebrospinal fluid are elevated in fed state and addition of lactate to glucose activates the majority of POMC neurons while increasing cytosolic NADH generation, mitochondrial respiration and extracellular pyruvate levels. Inhibition of lactate dehydrogenases diminishes mitochondrial respiration, NADH production, and POMC neuronal activity. However, inhibition of the mitochondrial pyruvate carrier has no effect. POMC-specific downregulation of Ucp2 (Ucp2PomcKO), a molecule regulated by fatty acid metabolism and shown to play a role as transporter in the malate-aspartate shuttle, abolishes lactate- and glucose-sensing of POMC neurons. Ucp2PomcKO mice have impaired glucose metabolism and are prone to obesity on a high fat diet. Altogether, our data show that lactate through redox signaling and blocking mitochondrial glucose utilization activates POMC neurons to regulate feeding and glucose metabolism.","INSTITUTE":"Columbia University","LAST_NAME":"Diano","FIRST_NAME":"Sabrina","ADDRESS":"1150 St. Nicholas Avenue Russ Berrie Medical Science Pavilion Rm 405 New York, NY, 10032","EMAIL":"sd3449@cumc.columbia.edu","PHONE":"212 8514554","SUBMIT_DATE":"2022-11-28"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Ucp2PomcKO_1_HC_Neg",
"Factors":{"Genotype":"UCP2 POMC-knockout"},
"Additional sample data":{"Instrument run modes":"Hypercarb column negative MS mode (HC_Neg)","RAW_FILE_NAME":"run15-16Sep22_KO_GlcNA_LacNA_1_HC_Neg.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Ucp2PomcKO_1_RP_Pos",
"Factors":{"Genotype":"UCP2 POMC-knockout"},
"Additional sample data":{"Instrument run modes":"Reverse phase column positive MS mode (RP_Pos)","RAW_FILE_NAME":"run15-15Sep22_KO_GlcNA_LacNA_1_RP_Pos.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Ucp2PomcKO_2_HC_Neg",
"Factors":{"Genotype":"UCP2 POMC-knockout"},
"Additional sample data":{"Instrument run modes":"Hypercarb column negative MS mode (HC_Neg)","RAW_FILE_NAME":"run16-16Sep22_KO_GlcNA_LacNA_2_HC_Neg.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Ucp2PomcKO_2_RP_Pos",
"Factors":{"Genotype":"UCP2 POMC-knockout"},
"Additional sample data":{"Instrument run modes":"Reverse phase column positive MS mode (RP_Pos)","RAW_FILE_NAME":"run16-15Sep22_KO_GlcNA_LacNA_2_RP_Pos.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Ctrl_1_HC_Neg",
"Factors":{"Genotype":"wild type"},
"Additional sample data":{"Instrument run modes":"Hypercarb column negative MS mode (HC_Neg)","RAW_FILE_NAME":"run6-16Sep22_Ctrl_GlcNA_LacNA_1_HC_Neg.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Ctrl_1_RP_Pos",
"Factors":{"Genotype":"wild type"},
"Additional sample data":{"Instrument run modes":"Reverse phase column positive MS mode (RP_Pos)","RAW_FILE_NAME":"run6-15Sep22_Ctrl_GlcNA_LacNA_1_RP_Pos.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Ctrl_2_HC_Neg",
"Factors":{"Genotype":"wild type"},
"Additional sample data":{"Instrument run modes":"Hypercarb column negative MS mode (HC_Neg)","RAW_FILE_NAME":"run25-16Sep22_Ctrl_GlcNA_LacNA_2_HC_Neg_rerun.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"Ctrl_2_RP_Pos",
"Factors":{"Genotype":"wild type"},
"Additional sample data":{"Instrument run modes":"Reverse phase column positive MS mode (RP_Pos)","RAW_FILE_NAME":"run7-15Sep22_Ctrl_GlcNA_LacNA_2_RP_Pos.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Hypothalamic primary neuronal cell culture Eight to ten neonatal (0 days old) pubs from either control or Ucp2PomcKO mice derived from homozygous Cre-positive parents were used for hypothalamic primary neuronal cell culture. In brief, we carefully removed the brain's hypothalamus and placed it onto a small culture dish containing a small volume of Hibernate-A Medium (Cat# A1247501, Thermo Fisher Scientific). After digestion, the tissues dissociated to single cells with 6 mL of Hibernate-A Medium containing 2.5 % of Trypsin-EDTA for 15 minutes at 37℃. Suspended cells were filtered (40 μm) and centrifuged for 5 min at 1000 rpm. The pellet was re-suspended and plated on XF96 cell culture microplates (Cat# 101085-004, Agilent Technologies) coated with poly-D-lysine (Cat# P6407, Sigma-Aldrich) at a density of 0.2 x104 cells per well. Cells were cultured in Neurobasal-A medium (Cat# 10888022, Thermo Fisher Scientific) supplemented with 1 % penicillin-streptomycin, 2 % B-27 Supplement (Cat# 17504044, Thermo Fisher Scientific), and GlutaMAX-I (Cat# 35050061, Thermo Fisher Scientific), CultureOne supplement (Cat# A3320201, Thermo Fisher Scientific). For control culture, we used either Ucp2fl/fl; Pomc-CreERT2; tdTomato mice which neuronal cultures were treated with vehicle (0.03 % ethanol diluted in medium) or Ucp2+/+; Pomc-CreERT2; tdTomato mice which neuronal cultures were treated with 2 μM 4-hydroxytamoxifen (Cat# H7904, Sigma-Aldrich). After 10 days in culture, primary neuronal cells isolated from control and Ucp2PomcKO mice were treated with 2 μM 4-hydroxytamoxifen for expression of a CreER recombinase. Primary neuronal cells were used for the measurement of mitochondria oxidation two days later.","SAMPLE_TYPE":"Neurons"},

"TREATMENT":{"TREATMENT_SUMMARY":"We used the inducible Cre/loxP technology to generate mice in which UCP2 was selectively ablated in POMC neurons (Ucp2PomcKO mice). First, mice expressing a tamoxifen-inducible Cre recombinase (CreERT2) in cells expressing POMC (Pomc-CreERT2) were crossed with Rosa26-lox-stop-lox-tdTomato (Ai14; cre-recombinase-dependent expression) mice (Ai14 reporter mice; stock #007914; The Jackson Laboratory) to label POMC-expressing cells. Pomc-CreERT2; Rosa26-lox-stop-lox-tdTomato (Pomc-CreERT2; tdTomato) mice have POMC-expressing cells with the expression of tdTomato by tamoxifen administration. No observation of POMC-tdTomato expression was found in the absence of tamoxifen administration, indicating that recombination was strictly dependent upon tamoxifen-induced Cre recombinase activation. The mice with Pomc-CreERT2; tdTomato were then crossed with mice harboring conditional alleles Ucp2 floxed (Ucp2fl/fl; B6;129S-Ucp2tm2.1Lowl/J, Stock# 022394; The Jackson Laboratory) to generated mice with inducible deletion of Ucp2 specifically in POMC neurons (Ucp2PomcKO mice). All animal experiments were conducted with the mice expressing POMC-CreERT2. As control groups, Ucp2fl/fl; Pomc-CreERT2 mice were injected with tamoxifen, and Ucp2fl/fl; Pomc-CreERT2; tdTomato were mice injected with corn oil (vehicle), and Ucp2fl/fl; Pomc-CreERT2; tdTomato mice were injected intraperitoneally (IP) with tamoxifen (0.1 mg/g BW for 5 consecutive days) starting at 5 weeks of age to induce mature-onset deletion of Ucp2 in POMC neurons of Ucp2PomcKO mice. However, because there were no differences between these two control groups, most of the experiments were performed using Ucp2+/+; Pomc-CreERT2; tdTomato mice injected with tamoxifen (to label POMC neurons with tdTomato expression) as a control group, unless otherwise stated. Body composition was measured in vivo by MRI (EchoMRI; Echo Medical Systems, Houston, TX) monthly at 10:00 AM. We performed transcriptomic profiling by using a ribosomal tagging strategy to analyze POMC neuron-specific mRNA expression in vivo. We crossed Pomc-CreERT2 mice30 with Rpl22 floxed (RiboTag, Stock# 029977, The Jackson Laboratories) mice to generate Pomc-CreERT2; RiboTag mice, expressing a hemagglutinin A (HA)-tagged ribosomal protein in the POMC neurons upon tamoxifen injection."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Cells cultured in 6-well plates were quickly washed once with ice cold 5 mM HEPES, then immediately quenched in 150ul/well ice cold quench buffer (20% methanol, 3 mM sodium fluoride, 0.1% formic acid, 1mM phenylalanine and 100uM EDTA). Each well was harvested using a cell lifter and immediately transferred to a pre-chilled 96-well plate on dry ice. Once completely frozen, cell lysates were lyophilized and stored in the -80oC freezer until the sample run. Lyophilized samples were prepared by resuspending in 50 µL water with D4-taurine (50 µM) as an internal standard and 5 µL of the supernatant was injected for each analysis mode using high performance liquid chromatography (HPLC) into the 6600 Triple TOF LC-MS/MS mass spectrometer (Sciex)."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Hypercarb column with negative MS mode","INSTRUMENT_NAME":"SCIEX ExionLC","COLUMN_NAME":"Thermo Scientific Hypercarb (100 x 4.6 mm,3um)","COLUMN_TEMPERATURE":"50","FLOW_RATE":"1ml/min","SOLVENT_A":"100% water; 15 mM ammonium formate; 0.03% acetyl acetone; 0.1% formic acid","SOLVENT_B":"60% acetonitrile/35% isopropanol; 0.1% formic acid; 15 mM ammonium formate","ANALYTICAL_TIME":"18min","WASHING_BUFFER":"50%Methanol, 50% water","CHROMATOGRAPHY_TYPE":"Other"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"MS_Analysis_Protocol.pdf"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex 6600 TripleTOF","INSTRUMENT_TYPE":"Triple TOF","MS_TYPE":"ESI","MS_COMMENTS":"Data were collected using an information-dependent analysis (IDA) workflow consisting of a TOF MS scan (200 msec) and a high-resolution IDA experiment (70 msec each) monitoring 10 candidate ions per cycle. Former target ions were excluded after 2 occurrences for 5 seconds and dynamic background subtraction was employed. The mass range for both TOF MS and IDA MS/MS scans was 70-1000. Ion spray voltage = -4500V, ion source gas 1 (GS1) = 50 psi, ion source gas 2 (GS2) = 50 psi, curtain gas (CUR) = 30 psi, temperature (TEM) = 500 oC.declustering potential (DP) = 35 V, collision energy (CE) = 30 V, collision energy spread (CES) = 20 V. El-MAVEN software (Elucidata.io) was used for peak picking and curation from house built targeted and untargeted libraries. Targeted libraries were developed using a commercial standard kit of ~600 metabolites (IROA Technologies) in each mode, yielding reference data (molecular ions and retention times) with wide coverage of the endogenous metabolome. Untargeted libraries contained ~2,700 metabolites drawn from the KEGG database where the top 5 candidates with the highest intensity throughout a sample run for each metabolite were automatically curated.","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"peak area",

"Data":[{"Metabolite":"10-HYDROXYDECANOATE","Ucp2PomcKO_1_HC_Neg":"2189.0000","Ucp2PomcKO_2_HC_Neg":"2781.0000","Ctrl_1_HC_Neg":"2410.0000","Ctrl_2_HC_Neg":"2795.0000"},{"Metabolite":"(2-AMINOETHYL)PHOSPHONATE","Ucp2PomcKO_1_HC_Neg":"5671.0000","Ucp2PomcKO_2_HC_Neg":"8785.0000","Ctrl_1_HC_Neg":"4349.0000","Ctrl_2_HC_Neg":"3760.0000"},{"Metabolite":"2-HYDROXYBUTYRATE","Ucp2PomcKO_1_HC_Neg":"519.0000","Ucp2PomcKO_2_HC_Neg":"680.0000","Ctrl_1_HC_Neg":"893.6000","Ctrl_2_HC_Neg":"648.2300"},{"Metabolite":"3-(2-HYDROXYPHENYL)PROPANOATE","Ucp2PomcKO_1_HC_Neg":"435197.5000","Ucp2PomcKO_2_HC_Neg":"655237.4400","Ctrl_1_HC_Neg":"674339.3100","Ctrl_2_HC_Neg":"401900.8100"},{"Metabolite":"3_4 DIHYDROXYMANDELATE","Ucp2PomcKO_1_HC_Neg":"58084.0000","Ucp2PomcKO_2_HC_Neg":"70620.4500","Ctrl_1_HC_Neg":"76693.0000","Ctrl_2_HC_Neg":"60630.4200"},{"Metabolite":"3_4-DIHYDROXYPHENYLGLYCOL","Ucp2PomcKO_1_HC_Neg":"6559934.5000","Ucp2PomcKO_2_HC_Neg":"8762054.0000","Ctrl_1_HC_Neg":"8526764.0000","Ctrl_2_HC_Neg":"6471353.0000"},{"Metabolite":"3-HYDROXYMETHYLGLUTARATE","Ucp2PomcKO_1_HC_Neg":"86030.2100","Ucp2PomcKO_2_HC_Neg":"151044.6400","Ctrl_1_HC_Neg":"165546.9100","Ctrl_2_HC_Neg":"100581.2700"},{"Metabolite":"3-METHOXY-4-HYDROXYMANDELATE","Ucp2PomcKO_1_HC_Neg":"394651.8800","Ucp2PomcKO_2_HC_Neg":"602151.2500","Ctrl_1_HC_Neg":"272594.0000","Ctrl_2_HC_Neg":"472837.9400"},{"Metabolite":"3-SULFINOALANINE","Ucp2PomcKO_1_HC_Neg":"3606.0000","Ucp2PomcKO_2_HC_Neg":"6168.0000","Ctrl_1_HC_Neg":"2760.0000","Ctrl_2_HC_Neg":"1590.0000"},{"Metabolite":"4-HYDROXYBENZALDEHYDE","Ucp2PomcKO_1_HC_Neg":"11990.7400","Ucp2PomcKO_2_HC_Neg":"9907.3900","Ctrl_1_HC_Neg":"10568.4400","Ctrl_2_HC_Neg":"9988.0500"},{"Metabolite":"5-AMINOLEVULINATE","Ucp2PomcKO_1_HC_Neg":"363.0000","Ucp2PomcKO_2_HC_Neg":"563.0000","Ctrl_1_HC_Neg":"520.0000","Ctrl_2_HC_Neg":"554.0000"},{"Metabolite":"5-AMINOPENTANOATE","Ucp2PomcKO_1_HC_Neg":"22908.0000","Ucp2PomcKO_2_HC_Neg":"24784.0000","Ctrl_1_HC_Neg":"13146.0000","Ctrl_2_HC_Neg":"16534.0000"},{"Metabolite":"6-CARBOXYHEXANOATE","Ucp2PomcKO_1_HC_Neg":"32817.7200","Ucp2PomcKO_2_HC_Neg":"35705.7100","Ctrl_1_HC_Neg":"28509.2500","Ctrl_2_HC_Neg":"37316.8100"},{"Metabolite":"6-PHOSPHOGLUCONATE (1)","Ucp2PomcKO_1_HC_Neg":"2545.0000","Ucp2PomcKO_2_HC_Neg":"3539.0000","Ctrl_1_HC_Neg":"1499.0000","Ctrl_2_HC_Neg":"1494.0000"},{"Metabolite":"AMINOISOBUTANOATE","Ucp2PomcKO_1_HC_Neg":"360.0000","Ucp2PomcKO_2_HC_Neg":"248.0000","Ctrl_1_HC_Neg":"199.0000","Ctrl_2_HC_Neg":"193.0000"},{"Metabolite":"ANSERINE","Ucp2PomcKO_1_HC_Neg":"47139.0000","Ucp2PomcKO_2_HC_Neg":"42911.0000","Ctrl_1_HC_Neg":"39051.0000","Ctrl_2_HC_Neg":"38918.0000"},{"Metabolite":"ARGININE","Ucp2PomcKO_1_HC_Neg":"65925.0000","Ucp2PomcKO_2_HC_Neg":"69933.0000","Ctrl_1_HC_Neg":"45754.0000","Ctrl_2_HC_Neg":"43034.0000"},{"Metabolite":"ASPARAGINE","Ucp2PomcKO_1_HC_Neg":"4382.0000","Ucp2PomcKO_2_HC_Neg":"3915.0000","Ctrl_1_HC_Neg":"1042.0000","Ctrl_2_HC_Neg":"1311.0000"},{"Metabolite":"AZELATE","Ucp2PomcKO_1_HC_Neg":"8218.8200","Ucp2PomcKO_2_HC_Neg":"9666.9800","Ctrl_1_HC_Neg":"10015.3400","Ctrl_2_HC_Neg":"9213.3200"},{"Metabolite":"BETAINE","Ucp2PomcKO_1_HC_Neg":"22908.0000","Ucp2PomcKO_2_HC_Neg":"24784.0000","Ctrl_1_HC_Neg":"13146.0000","Ctrl_2_HC_Neg":"16534.0000"},{"Metabolite":"CARNOSINE","Ucp2PomcKO_1_HC_Neg":"10408.4600","Ucp2PomcKO_2_HC_Neg":"8354.1300","Ctrl_1_HC_Neg":"1212.2000","Ctrl_2_HC_Neg":"2021.3400"},{"Metabolite":"CDP-ETHANOLAMINE","Ucp2PomcKO_1_HC_Neg":"20060.0000","Ucp2PomcKO_2_HC_Neg":"19825.0000","Ctrl_1_HC_Neg":"15278.0000","Ctrl_2_HC_Neg":"13965.0000"},{"Metabolite":"CIS-4-HYDROXY-D-PROLINE","Ucp2PomcKO_1_HC_Neg":"363.0000","Ucp2PomcKO_2_HC_Neg":"563.0000","Ctrl_1_HC_Neg":"520.0000","Ctrl_2_HC_Neg":"554.0000"},{"Metabolite":"CITICOLINE","Ucp2PomcKO_1_HC_Neg":"3919.4600","Ucp2PomcKO_2_HC_Neg":"2976.7400","Ctrl_1_HC_Neg":"4785.3200","Ctrl_2_HC_Neg":"5206.6000"},{"Metabolite":"CITRAMALATE","Ucp2PomcKO_1_HC_Neg":"152808.8300","Ucp2PomcKO_2_HC_Neg":"205128.0300","Ctrl_1_HC_Neg":"150681.8000","Ctrl_2_HC_Neg":"162480.0600"},{"Metabolite":"CITRATE","Ucp2PomcKO_1_HC_Neg":"1031823.0000","Ucp2PomcKO_2_HC_Neg":"1061641.0000","Ctrl_1_HC_Neg":"691807.0000","Ctrl_2_HC_Neg":"836070.0000"},{"Metabolite":"CORTISONE","Ucp2PomcKO_1_HC_Neg":"8862.2800","Ucp2PomcKO_2_HC_Neg":"8408.8900","Ctrl_1_HC_Neg":"2971.9400","Ctrl_2_HC_Neg":"2730.9500"},{"Metabolite":"CREATINE","Ucp2PomcKO_1_HC_Neg":"1482.0300","Ucp2PomcKO_2_HC_Neg":"1356.0000","Ctrl_1_HC_Neg":"854.0000","Ctrl_2_HC_Neg":"872.2000"},{"Metabolite":"CYSTEATE","Ucp2PomcKO_1_HC_Neg":"162374.0000","Ucp2PomcKO_2_HC_Neg":"260231.0000","Ctrl_1_HC_Neg":"154414.0000","Ctrl_2_HC_Neg":"72892.0000"},{"Metabolite":"CYSTEINE","Ucp2PomcKO_1_HC_Neg":"1638.0000","Ucp2PomcKO_2_HC_Neg":"2430.0000","Ctrl_1_HC_Neg":"1290.0000","Ctrl_2_HC_Neg":"1354.0000"},{"Metabolite":"CYSTEINE (1)","Ucp2PomcKO_1_HC_Neg":"1582.0000","Ucp2PomcKO_2_HC_Neg":"1431.0000","Ctrl_1_HC_Neg":"505.0000","Ctrl_2_HC_Neg":"797.0000"},{"Metabolite":"CYSTINE","Ucp2PomcKO_1_HC_Neg":"15899.0000","Ucp2PomcKO_2_HC_Neg":"28717.0000","Ctrl_1_HC_Neg":"14732.0000","Ctrl_2_HC_Neg":"14325.0000"},{"Metabolite":"D4-TAURINE","Ucp2PomcKO_1_HC_Neg":"250963.0000","Ucp2PomcKO_2_HC_Neg":"329146.1200","Ctrl_1_HC_Neg":"305970.7200","Ctrl_2_HC_Neg":"283350.0300"},{"Metabolite":"DEOXYADENOSINE","Ucp2PomcKO_1_HC_Neg":"40105.6700","Ucp2PomcKO_2_HC_Neg":"49182.4000","Ctrl_1_HC_Neg":"44846.8700","Ctrl_2_HC_Neg":"44352.9600"},{"Metabolite":"DEOXYGUANOSINE","Ucp2PomcKO_1_HC_Neg":"7010.7600","Ucp2PomcKO_2_HC_Neg":"9924.6000","Ctrl_1_HC_Neg":"8075.8800","Ctrl_2_HC_Neg":"6687.8300"},{"Metabolite":"DEOXYGUANOSINE-MONOPHOSPHATE","Ucp2PomcKO_1_HC_Neg":"926608.3800","Ucp2PomcKO_2_HC_Neg":"903649.3100","Ctrl_1_HC_Neg":"505776.4400","Ctrl_2_HC_Neg":"491006.4400"},{"Metabolite":"DETHIOBIOTIN","Ucp2PomcKO_1_HC_Neg":"2722.0000","Ucp2PomcKO_2_HC_Neg":"1515.0000","Ctrl_1_HC_Neg":"2869.0000","Ctrl_2_HC_Neg":"2130.0000"},{"Metabolite":"DIAMINOPIMELATE","Ucp2PomcKO_1_HC_Neg":"16063.0000","Ucp2PomcKO_2_HC_Neg":"9674.0000","Ctrl_1_HC_Neg":"5199.0000","Ctrl_2_HC_Neg":"3622.0000"},{"Metabolite":"DIHYDROXYACETONE PHOSPHATE","Ucp2PomcKO_1_HC_Neg":"6567.0000","Ucp2PomcKO_2_HC_Neg":"21130.0000","Ctrl_1_HC_Neg":"5938.0000","Ctrl_2_HC_Neg":"2574.0000"},{"Metabolite":"D-PINITOL","Ucp2PomcKO_1_HC_Neg":"75594.0000","Ucp2PomcKO_2_HC_Neg":"82929.0000","Ctrl_1_HC_Neg":"51274.0000","Ctrl_2_HC_Neg":"71875.0000"},{"Metabolite":"D-RIBOSE 5-PHOSPHATE","Ucp2PomcKO_1_HC_Neg":"5809.0000","Ucp2PomcKO_2_HC_Neg":"8363.0000","Ctrl_1_HC_Neg":"3741.0000","Ctrl_2_HC_Neg":"2862.0000"},{"Metabolite":"D-SEDOHEPTULOSE","Ucp2PomcKO_1_HC_Neg":"3799.0000","Ucp2PomcKO_2_HC_Neg":"7159.0000","Ctrl_1_HC_Neg":"4739.0000","Ctrl_2_HC_Neg":"7218.0000"},{"Metabolite":"FERULATE","Ucp2PomcKO_1_HC_Neg":"926892.0600","Ucp2PomcKO_2_HC_Neg":"1189551.0000","Ctrl_1_HC_Neg":"1380037.0000","Ctrl_2_HC_Neg":"871674.1900"},{"Metabolite":"FRUCTOSE 6-PHOSPHATE","Ucp2PomcKO_1_HC_Neg":"5928.0000","Ucp2PomcKO_2_HC_Neg":"8323.0000","Ctrl_1_HC_Neg":"4208.0000","Ctrl_2_HC_Neg":"2973.0000"},{"Metabolite":"FRUCTOSE BISPHOSPHATE","Ucp2PomcKO_1_HC_Neg":"45195.0000","Ucp2PomcKO_2_HC_Neg":"55829.0000","Ctrl_1_HC_Neg":"26608.0000","Ctrl_2_HC_Neg":"24411.0000"},{"Metabolite":"FUMARATE","Ucp2PomcKO_1_HC_Neg":"11208.8400","Ucp2PomcKO_2_HC_Neg":"12021.7900","Ctrl_1_HC_Neg":"7248.5900","Ctrl_2_HC_Neg":"6233.6500"},{"Metabolite":"G6P and G1P","Ucp2PomcKO_1_HC_Neg":"29342.0000","Ucp2PomcKO_2_HC_Neg":"34649.0000","Ctrl_1_HC_Neg":"15700.0000","Ctrl_2_HC_Neg":"16260.0000"},{"Metabolite":"GALACTOSE 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}