{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002378","ANALYSIS_ID":"AN003875","VERSION":"1","CREATED_ON":"12-15-2022"},

"PROJECT":{"PROJECT_TITLE":"Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation","PROJECT_TYPE":"Basic research","PROJECT_SUMMARY":"Activating macrophage NLRP3 inflammasome can promote excessive inflammation, with severe cell and tissue damage and organ dysfunction. Here, we show that pharmacological or genetic inhibition of pyruvate dehydrogenase kinase (PDHK) significantly attenuates NLRP3 inflammasome activation in murine and human macrophages and septic mice by lowering caspase-1 cleavage and IL-1beta secretion. Inhibiting PDHK reverses NLRP3 inflammasome-induced metabolic reprogramming, enhances autophagy, promotes mitochondrial fusion over fission, preserves cristae ultrastructure, and attenuates mitochondrial ROS production. The suppressive effect of PDHK inhibition on the NLRP3 inflammasome is independent of its canonical role as a pyruvate dehydrogenase regulator. We suggest that PDHK inhibition improves mitochondrial fitness by reversing NLRP3 inflammasome activation in acutely inflamed macrophages.","INSTITUTE":"Wake Forest School of Medicine","LAST_NAME":"Zhu","FIRST_NAME":"Xuewei","ADDRESS":"575 Patterson Ave","EMAIL":"xwzhu@wakehealth.edu","PHONE":"3367131445","DOI":"http://dx.doi.org/10.21228/M8Q13W"},

"STUDY":{"STUDY_TITLE":"Targeted metabolomics analysis of WT and GSDMDKO macrophages","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"Activating macrophage NLRP3 inflammasome can promote excessive inflammation, with severe cell and tissue damage and organ dysfunction. Here, we show that pharmacological or genetic inhibition of pyruvate dehydrogenase kinase (PDHK) significantly attenuates NLRP3 inflammasome activation in murine and human macrophages and septic mice by lowering caspase-1 cleavage and IL-1beta secretion. Inhibiting PDHK reverses NLRP3 inflammasome-induced metabolic reprogramming, enhances autophagy, promotes mitochondrial fusion over fission, preserves cristae ultrastructure, and attenuates mitochondrial ROS production. The suppressive effect of PDHK inhibition on the NLRP3 inflammasome is independent of its canonical role as a pyruvate dehydrogenase regulator. We suggest that PDHK inhibition improves mitochondrial fitness by reversing NLRP3 inflammasome activation in acutely inflamed macrophages.","INSTITUTE":"Wake Forest School of Medicine","LAST_NAME":"Zhu","FIRST_NAME":"Xuewei","ADDRESS":"575 Patterson Ave, Winston-Salem, NC 27101","EMAIL":"xwzhu@wakehealth.edu","PHONE":"3367131445","SUBMIT_DATE":"2022-11-16"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Male and female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"GSDMD-LPS-1",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-LPS-1.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-LPS-2",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-LPS-2.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-LPS-3",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-LPS-3.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-LPS-4",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-LPS-4.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-ATP-1",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-ATP-1.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-ATP-2",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-ATP-2.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-ATP-3",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-ATP-3.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-ATP-4",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-ATP-4.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-JX-1",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS+JX06+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-JX-1.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-JX-2",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS+JX06+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-JX-2.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-JX-3",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS+JX06+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-JX-3.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-JX-4",
"Factors":{"Genotype":"GSDMDKO","Treatment":"LPS+JX06+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-JX-4.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-control-1",
"Factors":{"Genotype":"GSDMDKO","Treatment":"no treatment"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-control-1.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-control-2",
"Factors":{"Genotype":"GSDMDKO","Treatment":"no treatment"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-control-2.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-control-3",
"Factors":{"Genotype":"GSDMDKO","Treatment":"no treatment"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-control-3.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"GSDMD-control-4",
"Factors":{"Genotype":"GSDMDKO","Treatment":"no treatment"},
"Additional sample data":{"RAW_FILE_NAME":"GSDMD-control-4.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-LPS-1",
"Factors":{"Genotype":"WT","Treatment":"LPS"},
"Additional sample data":{"RAW_FILE_NAME":"WT-LPS-1.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-LPS-2",
"Factors":{"Genotype":"WT","Treatment":"LPS"},
"Additional sample data":{"RAW_FILE_NAME":"WT-LPS-2.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-LPS-3",
"Factors":{"Genotype":"WT","Treatment":"LPS"},
"Additional sample data":{"RAW_FILE_NAME":"WT-LPS-3.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-LPS-4",
"Factors":{"Genotype":"WT","Treatment":"LPS"},
"Additional sample data":{"RAW_FILE_NAME":"WT-LPS-4.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-ATP-1",
"Factors":{"Genotype":"WT","Treatment":"LPS+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"WT-ATP-1.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-ATP-2",
"Factors":{"Genotype":"WT","Treatment":"LPS+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"WT-ATP-2.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-ATP-3",
"Factors":{"Genotype":"WT","Treatment":"LPS+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"WT-ATP-3.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-ATP-4",
"Factors":{"Genotype":"WT","Treatment":"LPS+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"WT-ATP-4.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-JX-1",
"Factors":{"Genotype":"WT","Treatment":"LPS+JX06+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"WT-JX-1.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-JX-2",
"Factors":{"Genotype":"WT","Treatment":"LPS+JX06+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"WT-JX-2.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-JX-3",
"Factors":{"Genotype":"WT","Treatment":"LPS+JX06+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"WT-JX-3.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-JX-4",
"Factors":{"Genotype":"WT","Treatment":"LPS+JX06+ATP"},
"Additional sample data":{"RAW_FILE_NAME":"WT-JX-4.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-control-1",
"Factors":{"Genotype":"WT","Treatment":"no treatment"},
"Additional sample data":{"RAW_FILE_NAME":"WT-control-1.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-control-2",
"Factors":{"Genotype":"WT","Treatment":"no treatment"},
"Additional sample data":{"RAW_FILE_NAME":"WT-control-2.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-control-3",
"Factors":{"Genotype":"WT","Treatment":"no treatment"},
"Additional sample data":{"RAW_FILE_NAME":"WT-control-3.mzml"}
},
{
"Subject ID":"-",
"Sample ID":"WT-control-4",
"Factors":{"Genotype":"WT","Treatment":"no treatment"},
"Additional sample data":{"RAW_FILE_NAME":"WT-control-4.mzml"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Macrophages were lysed, and polar metabolites were extracted using methanol and H2O (80:20; HPLC Grade; Sigma-Aldrich). Briefly, After treatment, immediately aspirate medium at room temperature. Immediately place the plate on dry ice, and add 1 mL 80% methanol/water (both HPLC grade) (pre-cooled in -80oC for at least 1hr).Remove the plate from -80oC freezer and put it on dry ice, scrape cells into extraction solvent. Transfer the whole cell extract to a new Eppendorf tube placed on ice. Centrifuge at 20 000 rcf for 10 min, 4oC.Transfer the supernatant into two tubes and dry with a speed vacuum at room temperature.","SAMPLE_TYPE":"Macrophages","STORAGE_CONDITIONS":"Described in summary"},

"TREATMENT":{"TREATMENT_SUMMARY":"WT and GSDMD KO bone marrow derived macrophages were first primed with 300 ng/ml LPS (E. coli 0111; B4, Sigma-Aldrich) before stimulated with or without 5 mM ATP (Sigma-Aldrich) for 30 min in the presence or absence of 10 uM JX06. Here, we show that pharmacological or genetic inhibition of pyruvate dehydrogenase kinase (PDHK) significantly attenuates NLRP3 inflammasome activation in murine and human macrophages and septic mice by lowering caspase-1 cleavage and IL-1beta secretion. Inhibiting PDHK reverses NLRP3 inflammasome-induced metabolic reprogramming, enhances autophagy, promotes mitochondrial fusion over fission, preserves cristae ultrastructure, and attenuates mitochondrial ROS production. The suppressive effect of PDHK inhibition on the NLRP3 inflammasome is independent of its canonical role as a pyruvate dehydrogenase regulator. We suggest that PDHK inhibition improves mitochondrial fitness by reversing NLRP3 inflammasome activation in acutely inflamed macrophages.","TREATMENT":"In vitro culture","TREATMENT_COMPOUND":"LPS, ATP, JX06"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Macrophages were lysed, and polar metabolites were extracted using methanol and H2O (80:20; HPLC Grade; Sigma-Aldrich). Briefly, After treatment, immediately aspirate medium at room temperature. Immediately place the plate on dry ice, and add 1 mL 80% methanol/water (both HPLC grade) (pre-cooled in -80oC for at least 1hr).Remove the plate from -80oC freezer and put it on dry ice, scrape cells into extraction solvent. Transfer the whole cell extract to a new Eppendorf tube placed on ice. Centrifuge at 20 000 rcf for 10 min, 4oC.Transfer the supernatant into two tubes and dry with a speed vacuum at room temperature. The dried metabolites were stored at -80 freezer before analysis.","PROCESSING_STORAGE_CONDITIONS":"Described in summary","EXTRACT_STORAGE":"Described in summary"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"500 μL of cell extract and 20 μL of MES (Thermo Fisher Scientific, Waltham, MA, USA) internal standard solution (10 ng/μL) were combined and mixed thoroughly through vortexing. The mixture was then dried under vacuum and reconstituted for analysis in 100 μL of ultrapure water (Optima, Thermo Fisher Scientific, Waltham, MA, USA). The analysis was performed on a Shimadzu Nexera UHPLC system coupled with a Shimadzu LCMS-8050 triple-quadrupole mass spectrometer (Kyoto, Japan). Two LC-MS/MS methods were employed to measure the targets. Ion-Pairing Separation was performed at 0.3 ml/min on a Zorbax Eclipse Plus C18 column (1.8 μm, 2.1 x 100 mm; Agilent, Santa Clara, CA USA). Mobile phase A consisted of ultrapure water (Optima, Thermo Fisher Scientific, Waltham, MA, USA) with 10 mM ammonium acetate (J.T. Baker, Thermo Fisher Scientific, Waltham, MA, USA) and 10 mM tributylamine (Acros Organics, Thermo Fisher Scientific, Fair Lawn NJ, USA) and mobile phase B consisted of methanol (Optima, Thermo Fisher Scientific, Waltham, MA, USA). The separation used the following gradient profile: 2 minutes at 0% B, a ramp to 25% B at 8 minutes, another ramp to 98%B at 12 minutes, a 3 minute hold until 15 minutes, and then a drop back to 0% B at 15.1 minutes and allowed to equilibrate there until 25 minutes. All analytes were monitored in negative mode.","INSTRUMENT_NAME":"Shimadzu Nexera X2","COLUMN_NAME":"Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um)","FLOW_GRADIENT":"The separation used the following gradient profile: 2 minutes at 0% B, a ramp to 25% B at 8 minutes, another ramp to 98%B at 12 minutes, a 3 minute hold until 15 minutes, and then a drop back to 0% B at 15.1 minutes and allowed to equilibrate there until 25 minutes.","SOLVENT_A":"100% water; 10 mM ammonium acetate; 10 mM tributylamine","SOLVENT_B":"100% methanol","CHROMATOGRAPHY_TYPE":"Ion pair"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Shimadzu Nexera X2","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","MS_COMMENTS":"The analysis was performed on a Shimadzu Nexera UHPLC system coupled with a Shimadzu LCMS-8050 triple-quadrupole mass spectrometer (Kyoto, Japan). Two LC-MS/MS methods were employed to measure the targets.","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"Peak area ratio",

"Data":[{"Metabolite":"2_3-bis-Phosphoglycerate","GSDMD-LPS-1":"0.0170","GSDMD-LPS-2":"0.0250","GSDMD-LPS-3":"0.0190","GSDMD-LPS-4":"0.0180","GSDMD-ATP-1":"0.7740","GSDMD-ATP-2":"1.4330","GSDMD-ATP-3":"1.2100","GSDMD-ATP-4":"1.1290","GSDMD-JX-1":"0.8470","GSDMD-JX-2":"0.6020","GSDMD-JX-3":"1.1140","GSDMD-JX-4":"1.3360","GSDMD-control-1":"0.0190","GSDMD-control-2":"0.0200","GSDMD-control-3":"0.0430","GSDMD-control-4":"0.0370","WT-LPS-1":"0.0250","WT-LPS-2":"0.0590","WT-LPS-3":"0.0230","WT-LPS-4":"0.0290","WT-ATP-1":"0.7440","WT-ATP-2":"0.8030","WT-ATP-3":"0.5900","WT-ATP-4":"0.3870","WT-JX-1":"0.7860","WT-JX-2":"1.1090","WT-JX-3":"0.8520","WT-JX-4":"0.8160","WT-control-1":"0.0140","WT-control-2":"0.0360","WT-control-3":"0.0230","WT-control-4":"0.0070"},{"Metabolite":"3-Phosphoglycerate/2-Phosphoglycerate","GSDMD-LPS-1":"0.0590","GSDMD-LPS-2":"0.0660","GSDMD-LPS-3":"0.0800","GSDMD-LPS-4":"0.0500","GSDMD-ATP-1":"1.3520","GSDMD-ATP-2":"1.6860","GSDMD-ATP-3":"1.4040","GSDMD-ATP-4":"1.8720","GSDMD-JX-1":"1.4860","GSDMD-JX-2":"0.5650","GSDMD-JX-3":"2.1930","GSDMD-JX-4":"2.0280","GSDMD-control-1":"0.1080","GSDMD-control-2":"0.0820","GSDMD-control-3":"0.0590","GSDMD-control-4":"0.0620","WT-LPS-1":"0.0440","WT-LPS-2":"0.1160","WT-LPS-3":"0.0480","WT-LPS-4":"0.0620","WT-ATP-1":"0.9140","WT-ATP-2":"0.7550","WT-ATP-3":"1.1070","WT-ATP-4":"0.5370","WT-JX-1":"1.2930","WT-JX-2":"1.3640","WT-JX-3":"1.7130","WT-JX-4":"1.5530","WT-control-1":"0.0720","WT-control-2":"0.0940","WT-control-3":"0.0760","WT-control-4":"0.0630"},{"Metabolite":"Fructose-1_6-bis-phosphate","GSDMD-LPS-1":"0.1410","GSDMD-LPS-2":"0.1710","GSDMD-LPS-3":"0.1350","GSDMD-LPS-4":"0.0690","GSDMD-ATP-1":"1.7660","GSDMD-ATP-2":"2.5070","GSDMD-ATP-3":"2.1990","GSDMD-ATP-4":"2.4570","GSDMD-JX-1":"1.8780","GSDMD-JX-2":"1.3190","GSDMD-JX-3":"3.3510","GSDMD-JX-4":"2.6440","GSDMD-control-1":"0.1730","GSDMD-control-2":"0.1400","GSDMD-control-3":"0.1800","GSDMD-control-4":"0.0580","WT-LPS-1":"0.1420","WT-LPS-2":"0.2140","WT-LPS-3":"0.0920","WT-LPS-4":"0.1960","WT-ATP-1":"1.1360","WT-ATP-2":"0.9340","WT-ATP-3":"1.3920","WT-ATP-4":"0.9660","WT-JX-1":"1.7020","WT-JX-2":"2.2370","WT-JX-3":"2.4240","WT-JX-4":"1.7630","WT-control-1":"0.1300","WT-control-2":"0.1320","WT-control-3":"0.0330","WT-control-4":"0.0380"},{"Metabolite":"Glucose-1-phosphate","GSDMD-LPS-1":"0.0260","GSDMD-LPS-2":"0.0360","GSDMD-LPS-3":"0.0460","GSDMD-LPS-4":"0.0690","GSDMD-ATP-1":"0.0870","GSDMD-ATP-2":"0.1120","GSDMD-ATP-3":"0.1480","GSDMD-ATP-4":"0.1220","GSDMD-JX-1":"0.1100","GSDMD-JX-2":"0.0720","GSDMD-JX-3":"0.1450","GSDMD-JX-4":"0.1810","GSDMD-control-1":"0.0170","GSDMD-control-2":"0.0110","GSDMD-control-3":"0.0230","GSDMD-control-4":"0.0090","WT-LPS-1":"0.0090","WT-LPS-2":"0.0160","WT-LPS-3":"0.0370","WT-LPS-4":"0.0240","WT-ATP-1":"0.2200","WT-ATP-2":"0.2350","WT-ATP-3":"0.2370","WT-ATP-4":"0.1430","WT-JX-1":"0.1280","WT-JX-2":"0.1290","WT-JX-3":"0.1650","WT-JX-4":"0.1540","WT-control-1":"0.0090","WT-control-2":"0.0120","WT-control-3":"0.0160","WT-control-4":"0.0160"},{"Metabolite":"Glucose-6-phosphate/Fructose-6-phosphate","GSDMD-LPS-1":"0.0470","GSDMD-LPS-2":"0.0270","GSDMD-LPS-3":"0.0760","GSDMD-LPS-4":"0.1570","GSDMD-ATP-1":"0.1870","GSDMD-ATP-2":"0.2120","GSDMD-ATP-3":"0.2220","GSDMD-ATP-4":"0.2500","GSDMD-JX-1":"0.2160","GSDMD-JX-2":"0.1360","GSDMD-JX-3":"0.2890","GSDMD-JX-4":"0.3520","GSDMD-control-1":"0.0120","GSDMD-control-2":"0.0360","GSDMD-control-3":"0.0280","GSDMD-control-4":"0.0210","WT-LPS-1":"0.0100","WT-LPS-2":"0.0390","WT-LPS-3":"0.0580","WT-LPS-4":"0.0440","WT-ATP-1":"0.5410","WT-ATP-2":"0.4890","WT-ATP-3":"0.5700","WT-ATP-4":"0.3200","WT-JX-1":"0.2780","WT-JX-2":"0.2670","WT-JX-3":"0.3100","WT-JX-4":"0.3140","WT-control-1":"0.0130","WT-control-2":"0.0150","WT-control-3":"0.0090","WT-control-4":"0.0280"},{"Metabolite":"Glyceraldehyde-3-phosphate/Dihydroxyacetonephosphate","GSDMD-LPS-1":"0.0170","GSDMD-LPS-2":"0.0280","GSDMD-LPS-3":"0.0270","GSDMD-LPS-4":"0.1020","GSDMD-ATP-1":"0.6500","GSDMD-ATP-2":"0.3270","GSDMD-ATP-3":"0.3300","GSDMD-ATP-4":"0.3300","GSDMD-JX-1":"0.8130","GSDMD-JX-2":"0.4700","GSDMD-JX-3":"0.8800","GSDMD-JX-4":"1.1540","GSDMD-control-1":"0.0040","GSDMD-control-2":"0.0110","GSDMD-control-3":"0.0090","GSDMD-control-4":"0.0200","WT-LPS-1":"0.0320","WT-LPS-2":"0.0340","WT-LPS-3":"0.0090","WT-LPS-4":"0.0260","WT-ATP-1":"1.0560","WT-ATP-2":"0.3540","WT-ATP-3":"0.5300","WT-ATP-4":"0.1780","WT-JX-1":"0.5000","WT-JX-2":"0.3730","WT-JX-3":"0.3980","WT-JX-4":"0.5000","WT-control-1":"0.0050","WT-control-2":"0.0030","WT-control-3":"0.0070","WT-control-4":"0.0060"},{"Metabolite":"Glycerol-3-phosphate","GSDMD-LPS-1":"0.1230","GSDMD-LPS-2":"0.1310","GSDMD-LPS-3":"0.1590","GSDMD-LPS-4":"0.1690","GSDMD-ATP-1":"0.0840","GSDMD-ATP-2":"0.0950","GSDMD-ATP-3":"0.1530","GSDMD-ATP-4":"0.1090","GSDMD-JX-1":"0.0960","GSDMD-JX-2":"0.0500","GSDMD-JX-3":"0.1350","GSDMD-JX-4":"0.1610","GSDMD-control-1":"0.0360","GSDMD-control-2":"0.0740","GSDMD-control-3":"0.0580","GSDMD-control-4":"0.0770","WT-LPS-1":"0.0860","WT-LPS-2":"0.0700","WT-LPS-3":"0.1190","WT-LPS-4":"0.1170","WT-ATP-1":"0.1220","WT-ATP-2":"0.1410","WT-ATP-3":"0.1300","WT-ATP-4":"0.0600","WT-JX-1":"0.1070","WT-JX-2":"0.0920","WT-JX-3":"0.1210","WT-JX-4":"0.1210","WT-control-1":"0.0210","WT-control-2":"0.0410","WT-control-3":"0.0290","WT-control-4":"0.0370"},{"Metabolite":"Phosphoenolpyruvate","GSDMD-LPS-1":"0.0200","GSDMD-LPS-2":"0.0310","GSDMD-LPS-3":"0.0100","GSDMD-LPS-4":"0.0080","GSDMD-ATP-1":"1.0290","GSDMD-ATP-2":"1.4210","GSDMD-ATP-3":"1.3000","GSDMD-ATP-4":"1.3740","GSDMD-JX-1":"1.1740","GSDMD-JX-2":"0.7530","GSDMD-JX-3":"1.6010","GSDMD-JX-4":"1.6700","GSDMD-control-1":"0.0140","GSDMD-control-2":"0.0060","GSDMD-control-3":"0.0110","GSDMD-control-4":"0.0050","WT-LPS-1":"0.0140","WT-LPS-2":"0.0360","WT-LPS-3":"0.0110","WT-LPS-4":"0.0260","WT-ATP-1":"0.7450","WT-ATP-2":"0.8790","WT-ATP-3":"0.8520","WT-ATP-4":"0.5170","WT-JX-1":"1.0730","WT-JX-2":"1.2860","WT-JX-3":"1.3660","WT-JX-4":"1.3690","WT-control-1":"0.0100","WT-control-2":"0.0150","WT-control-3":"0.0070","WT-control-4":"0.0050"}],

"Metabolites":[{"Metabolite":"2,3-bis-Phosphoglycerate","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"3-Phosphoglycerate/2-Phosphoglycerate","pubchem_id":"439278","inchi_key":"","kegg_id":"C00631","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Fructose-1,6-bis-phosphate","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Glucose-1-phosphate","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Glucose-6-phosphate/Fructose-6-phosphate","pubchem_id":"440641","inchi_key":"","kegg_id":"C05345","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Glyceraldehyde-3-phosphate/Dihydroxyacetonephosphate","pubchem_id":"668","inchi_key":"","kegg_id":"C00111","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Glycerol-3-phosphate","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Phosphoenolpyruvate","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"","ri":"","ri_type":"","moverz_quant":"","":""}]
}

}