{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002485","ANALYSIS_ID":"AN004057","VERSION":"1","CREATED_ON":"February 21, 2023, 7:51 am"},

"PROJECT":{"PROJECT_TITLE":"Simultaneous targeting of PD-1 and IL2Rβγ with radiation therapy to inhibit pancreatic cancer growth and metastasis","PROJECT_SUMMARY":"In pancreatic ductal adenocarcinoma (PDAC) patients, we show that response to radiation therapy (RT) is characterized by increased IL2Rβ and IL2Rγ and decreased ILR2α expression. The bispecific aPD1-IL2v is a PD-1-targeted IL-2 variant (IL2v) immunocytokine with engineered IL-2 cis-targeted to PD-1 and abolished IL2Rα binding, which enhances tumor-antigen specific T cell activation while reducing regulatory T cell (Treg) suppression. Using aPD1-IL2v in orthotopic PDAC KPC-driven tumor models, we show marked improvement in local and metastatic survival along with profound increase in tumor-infiltrating polyfunctional CD8+ T cell subsets with a transcriptionally and metabolically active phenotype, and preferential activation of antigen-specific CD8+ T cells. In combination with single dose RT, aPD1-IL2v treatment results in a robust, durable expansion of polyfunctional CD8+ T cells, T cell stemness, tumor-specific memory immune response, natural killer (NK) cell activation, and decreased Tregs. These data show that aPD1-IL2v leads to profound local and distant response in PDAC.","INSTITUTE":"University of Colorado Denver","LABORATORY":"Lab of Angelo D'Alessandro in collaboration with lab of Sana Karam","LAST_NAME":"Haines","FIRST_NAME":"Julie","ADDRESS":"12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA","EMAIL":"julie.haines@cuanschutz.edu","PHONE":"3037243339"},

"STUDY":{"STUDY_TITLE":"Simultaneous targeting of PD-1 and IL2Rβγ with radiation therapy to inhibit pancreatic cancer growth and metastasis - mouse serum metabolomics","STUDY_SUMMARY":"Mice were orthotopically implanted with PK5L1940 cells then radiation therapy (8 Gy) administered 7 days post-implantation. PD1-IL2v and aCD25 dosed once per week beginning day 7 post-implantation. Serum was collected at time of sacrifice and profiled by mass spectrometry-based metabolomics to visualize changes in systemic metabolism in the mouse model.","INSTITUTE":"University of Colorado Denver","LAST_NAME":"Haines","FIRST_NAME":"Julie","ADDRESS":"12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA","EMAIL":"julie.haines@cuanschutz.edu","PHONE":"3037243339"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"1",
"Factors":{"treatment":"RT"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-01_r19"}
},
{
"Subject ID":"-",
"Sample ID":"2",
"Factors":{"treatment":"RT"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-02_r3"}
},
{
"Subject ID":"-",
"Sample ID":"3",
"Factors":{"treatment":"RT"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-03_r22"}
},
{
"Subject ID":"-",
"Sample ID":"4",
"Factors":{"treatment":"RT+aCD25"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-04_r26"}
},
{
"Subject ID":"-",
"Sample ID":"5",
"Factors":{"treatment":"RT+aCD25"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-05_r16"}
},
{
"Subject ID":"-",
"Sample ID":"6",
"Factors":{"treatment":"RT+aCD25"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-06_r15"}
},
{
"Subject ID":"-",
"Sample ID":"7",
"Factors":{"treatment":"RT+IL2v"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-07_r20"}
},
{
"Subject ID":"-",
"Sample ID":"8",
"Factors":{"treatment":"RT+IL2v"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-08_r10"}
},
{
"Subject ID":"-",
"Sample ID":"9",
"Factors":{"treatment":"RT+IL2v"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-09_r12"}
},
{
"Subject ID":"-",
"Sample ID":"10",
"Factors":{"treatment":"RT+aCD25+IL2v"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-10_r27"}
},
{
"Subject ID":"-",
"Sample ID":"11",
"Factors":{"treatment":"RT+aCD25+IL2v"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-11_r17"}
},
{
"Subject ID":"-",
"Sample ID":"12",
"Factors":{"treatment":"RT+aCD25+IL2v"},
"Additional sample data":{"RAW_FILE_NAME":"BW53-12_r8"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Blood was collected at the time of sacrifice via cardiac puncture. Serum was isolated using BD microcontainer plasma separation collection tubes. Serum layer was harvested after centrifugation at 6000RPM, 4degC for 2mins, and flash frozen.","SAMPLE_TYPE":"Blood (serum)"},

"TREATMENT":{"TREATMENT_SUMMARY":"Mice were orthotopically implanted with PK5L1940 cells. RT administered 7 days post-implantation. PD1-IL2v and aCD25 dosed once per week beginning day 7 post-implantation. Serum collected at time of sacrifice."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Serum aliquots were thawed on ice then a 20 uL aliquot treated with 480 uL of cold 5:3:2 MeOH:acetonitrile:water. Samples were vortexed 30 min at 4 degrees C then supernatants clarified by centrifugation (10 min, 10,000 g, 4 degrees C) and transferred to autosampler vials.","PROCESSING_STORAGE_CONDITIONS":"4℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Positive C18","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Phenomenex Kinetex C18 2.1 x 150 mm, 1.7 um","SOLVENT_A":"100% water; 0.1% formic acid","SOLVENT_B":"100%acetonitrile; 0.1% formic acid","FLOW_GRADIENT":"0-0.5 min 5% B, 0.5-1.1 min 5-95% B, 1.1-2.75 min hold at 95% B, 2.75-3 min 95-5% B, 3-5 min hold at 5% B","FLOW_RATE":"450 uL/min","COLUMN_TEMPERATURE":"45","SAMPLE_INJECTION":"10 uL"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Resolution 70,000, scan range 65-900 m/z, maximum injection time 200 ms, microscans 2, automatic gain control (AGC) 3 x 10^6 ions, source voltage 4.0 kV, capillary temperature 320 C, and sheath gas 45, auxiliary gas 15, and sweep gas 0 (all nitrogen). Data converted to mzXML using RawConverter. Metabolites were annotated and integrated using Maven in conjunction with the KEGG database."},

"MS_METABOLITE_DATA":{
"Units":"peak area",

"Data":[{"Metabolite":"L-alanine","1":"15800180","2":"17823870","3":"10124510","4":"9727420","5":"7813568","6":"9785114","7":"10188390","8":"9498863","9":"10885160","10":"6914096","11":"6264812","12":"14011350"},{"Metabolite":"L-arginine","1":"10072410","2":"7968570","3":"83070.09","4":"10724460","5":"11478290","6":"16001950","7":"7536732","8":"11971180","9":"8950375","10":"9347515","11":"11710710","12":"13177410"},{"Metabolite":"L-leucine","1":"60649590","2":"50899730","3":"97859380","4":"54224510","5":"38984760","6":"62416210","7":"57792040","8":"55802820","9":"48364020","10":"39712310","11":"43805630","12":"65761870"},{"Metabolite":"L-lysine","1":"15903560","2":"13339560","3":"8703347","4":"8966953","5":"7791226","6":"8873349","7":"8399610","8":"7048130","9":"8599469","10":"8570831","11":"10189960","12":"9865579"},{"Metabolite":"L-methionine","1":"8784535","2":"11526430","3":"6571202","4":"8451901","5":"4301808","6":"10089530","7":"9762827","8":"9332828","9":"9452227","10":"4577828","11":"5665624","12":"10508000"},{"Metabolite":"L-phenylalanine","1":"9102805","2":"10560420","3":"10923730","4":"5637410","5":"4708020","6":"6282900","7":"6107718","8":"5984282","9":"5458164","10":"5100652","11":"4834254","12":"6865010"},{"Metabolite":"L-proline","1":"33187710","2":"39015810","3":"21818180","4":"19519960","5":"15384850","6":"26806980","7":"31180950","8":"26939960","9":"26125820","10":"13872200","11":"13947240","12":"29187940"},{"Metabolite":"L-serine","1":"675409.2","2":"988892.8","3":"843850.7","4":"631259.9","5":"447863.5","6":"741096.5","7":"689088.3","8":"645170.1","9":"695450.4","10":"492246.5","11":"533911.7","12":"829390.7"},{"Metabolite":"L-tyrosine","1":"7556930","2":"7760706","3":"9422472","4":"9212229","5":"5725142","6":"11060480","7":"8408149","8":"10199670","9":"8037282","10":"5566007","11":"6164634","12":"10433180"},{"Metabolite":"dAMP","1":"97352.06","2":"47291.85","3":"0","4":"132928.1","5":"75801.26","6":"98182.41","7":"61913.17","8":"77077.87","9":"89315.74","10":"96857.75","11":"99980.71","12":"74255.15"},{"Metabolite":"Adenosine","1":"5404.471","2":"1217.792","3":"2651.264","4":"3130.553","5":"1401.959","6":"3466.922","7":"2507.332","8":"3492.2","9":"4717.386","10":"1141.716","11":"2796.509","12":"1487.872"},{"Metabolite":"Guanine","1":"309425.7","2":"299575.8","3":"188008.5","4":"329572.2","5":"185063.8","6":"371359.8","7":"290488.2","8":"369437.6","9":"248053.7","10":"163664.7","11":"202689.1","12":"380645.3"},{"Metabolite":"Cytosine","1":"163622.5","2":"129270.4","3":"264669.6","4":"188648.1","5":"142906","6":"126128.4","7":"213864.9","8":"237007.6","9":"264417.8","10":"220827","11":"230447.1","12":"277055"},{"Metabolite":"Thymine","1":"142060.4","2":"127700.6","3":"113148.1","4":"136582.8","5":"128648.1","6":"85585.05","7":"92385.84","8":"84463.84","9":"99233.84","10":"78658.12","11":"86233.68","12":"95456.32"},{"Metabolite":"Uracil","1":"252328.2","2":"304603","3":"282062.5","4":"502016","5":"317565","6":"156197.4","7":"379457.6","8":"313446.7","9":"467565.2","10":"396249.6","11":"231251.9","12":"302982.2"},{"Metabolite":"Hypoxanthine","1":"7492.115","2":"27802.73","3":"3180.549","4":"620432.2","5":"73982.62","6":"1610326","7":"38293.97","8":"21440.18","9":"56764.92","10":"33723.41","11":"3852.226","12":"27556.03"},{"Metabolite":"Xanthine","1":"133303.9","2":"131521.4","3":"178725","4":"2635677","5":"83981.07","6":"919966.8","7":"116841.2","8":"87449.27","9":"83772.48","10":"153787.2","11":"152374.5","12":"71894.47"},{"Metabolite":"Allantoate","1":"1450495","2":"1586608","3":"3184196","4":"1027322","5":"953027.6","6":"731706.5","7":"1193424","8":"655928.4","9":"1147108","10":"789785.8","11":"568984.4","12":"1030114"},{"Metabolite":"5-Hydroxyisourate","1":"319004.2","2":"467126.8","3":"517530.5","4":"388169.6","5":"324577.8","6":"273524.8","7":"317033.5","8":"262931.4","9":"379580.2","10":"528290.2","11":"335498.1","12":"314322"},{"Metabolite":"Pyridoxal","1":"119841.3","2":"235678.7","3":"39321.25","4":"166709.4","5":"150732","6":"235085.5","7":"113673","8":"188137.8","9":"205073.5","10":"134958.6","11":"126114.7","12":"168142.1"},{"Metabolite":"4-Pyridoxate","1":"149427.7","2":"89438.62","3":"574328.7","4":"164666.3","5":"200599.9","6":"245014.6","7":"140709.2","8":"154018.7","9":"107323.8","10":"142744.6","11":"138671.9","12":"187581.2"},{"Metabolite":"Pyridoxamine 5'-phosphate","1":"678651.9","2":"423833.5","3":"446164.2","4":"706080.4","5":"1014012","6":"1414514","7":"922773.2","8":"1085519","9":"966741.1","10":"732274.7","11":"1056494","12":"1201684"},{"Metabolite":"Nicotinamide","1":"385321.8","2":"578533.7","3":"969605.2","4":"1088308","5":"629711.2","6":"775183.9","7":"1042039","8":"805212","9":"1043478","10":"850044.8","11":"547503.4","12":"1112353"},{"Metabolite":"Glutathione","1":"56091.46","2":"84400.43","3":"20740.51","4":"91829.38","5":"104372.9","6":"65013.71","7":"0","8":"92201.32","9":"120654.5","10":"96050.34","11":"69118.93","12":"67678.59"},{"Metabolite":"gamma-Glutamyl-Se-methylselenocysteine","1":"543703.6","2":"587287.9","3":"1449767","4":"411644.9","5":"413860.4","6":"254874.5","7":"529601.1","8":"254202","9":"468513.5","10":"314326.4","11":"274138.3","12":"364233.8"},{"Metabolite":"gamma-L-Glutamyl-D-alanine","1":"332354.9","2":"443817","3":"263645.9","4":"182618.4","5":"201247.6","6":"281555.1","7":"292966.3","8":"384674.2","9":"302831.4","10":"178320","11":"200629.6","12":"284367.5"},{"Metabolite":"Ornithine","1":"2102211","2":"2121771","3":"4665136","4":"1524641","5":"1247577","6":"883961.8","7":"1706421","8":"879524.5","9":"1729809","10":"1204048","11":"972127.2","12":"1279171"},{"Metabolite":"L-Citrulline","1":"1962625","2":"3590712","3":"2089418","4":"1881596","5":"1080799","6":"2342799","7":"1713092","8":"1606462","9":"2086814","10":"1216216","11":"1473773","12":"2100686"},{"Metabolite":"Putrescine","1":"101879.7","2":"94124.5","3":"147376.5","4":"55834.03","5":"53945","6":"40963.18","7":"41174.29","8":"52764.24","9":"79081.66","10":"96408.85","11":"57566.1","12":"43507"},{"Metabolite":"Spermidine","1":"98988.16","2":"109570.2","3":"275782.3","4":"349921.7","5":"99951.71","6":"63999.59","7":"124500","8":"109125.9","9":"152701.3","10":"113919.6","11":"71798.23","12":"84679.2"},{"Metabolite":"5-Guanidino-2-oxopentanoate","1":"37964.48","2":"33232.71","3":"62144.67","4":"195334.9","5":"105004.6","6":"85620.15","7":"88480.06","8":"79565.12","9":"196129.8","10":"85629.32","11":"83774.44","12":"93424.12"},{"Metabolite":"Phosphocreatine","1":"75725.62","2":"85472.44","3":"580740.1","4":"76659.85","5":"72173.46","6":"66659.79","7":"66163.91","8":"79382.48","9":"67461.34","10":"111318.8","11":"94595.62","12":"77583.1"},{"Metabolite":"Creatinine","1":"1436770","2":"3341532","3":"2254314","4":"1396172","5":"1219089","6":"1380517","7":"1472554","8":"1309160","9":"1636442","10":"1662436","11":"1512549","12":"1349851"},{"Metabolite":"N-Acetyl-L-citrulline","1":"23236.95","2":"30614.53","3":"48086.93","4":"85705.46","5":"46765.07","6":"40927.78","7":"38726.09","8":"36356.68","9":"85990.93","10":"35395.94","11":"37312.65","12":"41254.54"},{"Metabolite":"N-Acetylornithine","1":"34393.98","2":"114804","3":"189982","4":"135282.2","5":"117230.9","6":"192592.9","7":"152911","8":"168892","9":"126331","10":"98598.69","11":"123074.7","12":"166199.9"},{"Metabolite":"Pantothenate","1":"1529633","2":"2954867","3":"2698430","4":"1047119","5":"1193088","6":"819113.8","7":"1025729","8":"835644.1","9":"1245671","10":"950983.8","11":"549223.6","12":"1088994"},{"Metabolite":"5-6-Dihydrouracil","1":"458315.3","2":"253948.2","3":"581000.5","4":"653260.4","5":"714780.2","6":"457843.2","7":"1147595","8":"671648.1","9":"1193862","10":"1350784","11":"674764.6","12":"890505.1"},{"Metabolite":"Pantetheine","1":"73031.09","2":"203557.8","3":"33425.43","4":"67620.27","5":"36157.53","6":"46409.21","7":"74963.85","8":"54639.79","9":"70790.82","10":"29840.02","11":"19149.44","12":"94856.82"},{"Metabolite":"3-methyldioxyindole","1":"170725.9","2":"11222.02","3":"41872.58","4":"65159.46","5":"126085.7","6":"210961.2","7":"189920.9","8":"162750","9":"163128.4","10":"61558.41","11":"139875.7","12":"115110.2"},{"Metabolite":"Indole","1":"317976.9","2":"762405.1","3":"442282.2","4":"1045774","5":"735925.5","6":"471665","7":"557853.1","8":"570600.3","9":"1088742","10":"569273.7","11":"370244.4","12":"657745.2"},{"Metabolite":"Indole-3-acetaldehyde","1":"559843.7","2":"771560.6","3":"1384869","4":"3702147","5":"4558138","6":"9439970","7":"3144985","8":"3796877","9":"3353858","10":"3497950","11":"3881744","12":"1896672"},{"Metabolite":"Indole-3-acetate","1":"66177.8","2":"164498.9","3":"24432.24","4":"758360.4","5":"279088.5","6":"233309.4","7":"439280.1","8":"241718.4","9":"416017","10":"318066","11":"216456.1","12":"273136.5"},{"Metabolite":"6-Hydroxykynurenic acid","1":"61795.77","2":"129628.5","3":"54327.01","4":"46153.95","5":"33671.41","6":"39655.66","7":"79077.75","8":"48695.96","9":"64190.93","10":"12464.07","11":"26603.76","12":"56088.32"},{"Metabolite":"kynurenine","1":"200534","2":"133091.5","3":"522607.8","4":"208062.1","5":"146484.7","6":"149100.9","7":"178649.4","8":"113349.6","9":"211311.1","10":"130201.7","11":"240482.9","12":"165847.8"},{"Metabolite":"N-formyl kynurenine","1":"4038.529","2":"4954.532","3":"10519.55","4":"5668.666","5":"3093.27","6":"2703.931","7":"13855.11","8":"1813.113","9":"8408.371","10":"1445.937","11":"3662.701","12":"3306.986"},{"Metabolite":"Anthranilate","1":"741427.9","2":"4052574","3":"320458.8","4":"1255079","5":"697157.2","6":"1121237","7":"1193697","8":"1537376","9":"1073914","10":"596178.4","11":"809811","12":"1074667"},{"Metabolite":"Picolinic 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}

}