{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002526","ANALYSIS_ID":"AN004160","VERSION":"1","CREATED_ON":"March 28, 2023, 10:00 am"},

"PROJECT":{"PROJECT_TITLE":"Pancreatic tumors activate arginine biosynthesis to adapt to myeloid-driven amino acid stress","PROJECT_SUMMARY":"Nutrient stress in the tumor microenvironment requires cancer cells to adopt adaptive metabolic programs to maintain survival and proliferation. Therefore, knowledge of microenvironmental nutrient levels and how cancer cells cope with such nutrition is critical to understand the metabolism underpinning cancer cell biology. Previously, we performed quantitative metabolomics of the interstitial fluid (the local perfusate) of murine pancreatic ductal adenocarcinoma (PDAC) tumors to comprehensively characterize nutrient availability in the microenvironment of these tumors (Sullivan et al., 2019a). Here, we develop Tumor Interstitial Fluid Medium (TIFM), a cell culture medium that contains nutrient levels representative of the PDAC microenvironment, enabling study of PDAC metabolism under physiological nutrition. We show that PDAC cells cultured in TIFM, compared to standard laboratory models, adopt a cellular state more similar to PDAC cells in tumors. Further, using the TIFM model we identified arginine biosynthesis as a metabolic adaptation PDAC cells engage to cope with microenvironmental arginine starvation driven by myeloid cells in PDAC tumors. Altogether, these data show that nutrient availability in tumors is an important determinant of cancer cell metabolism and behavior, and cell culture models that incorporate physiological nutrient availability have improved fidelity and enable the discovery of novel cancer metabolic phenotypes.","INSTITUTE":"University of Chicago","LAST_NAME":"Apiz Saab","FIRST_NAME":"Juan","ADDRESS":"929 E. 57th St.","EMAIL":"japizsaab@uchicago.edu","PHONE":"7738346506"},

"STUDY":{"STUDY_TITLE":"Stable isotope tracing of 15N2-glutamine in orthotopic pancreatic ductal adenocarcinoma tumor bearing mice and non tumor-bearing controls","STUDY_SUMMARY":"Stable isotope tracing by bolus intravenous injections of 15N2-glutamine in orthotopic PDAC tumor bearing mice and non tumor-bearing controls followed by plasma sampling and tumor extraction for analysis of intratumoral metabolite labeling during the period of kinetic labeling","INSTITUTE":"University of Chicago","LAST_NAME":"Apiz Saab","FIRST_NAME":"Juan","ADDRESS":"929 E. 57th St.","EMAIL":"japizsaab@uchicago.edu","PHONE":"7738346506"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Tissue 1",
"Factors":{"Sample type":"Healthy_Mouse_Pancreas_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_01_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 2",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Tumor_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_02_PDAC-Tumor.raw.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 3",
"Factors":{"Sample type":"Healthy_Mouse_Pancreas_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_03_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 4",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Tumor_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_04_PDAC-Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 5",
"Factors":{"Sample type":"Healthy_Mouse_Pancreas_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_05_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 6",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Tumor_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_06_PDAC-Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 7",
"Factors":{"Sample type":"Healthy_Mouse_Pancreas_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_07_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 8",
"Factors":{"Sample type":"Healthy_Mouse_Pancreas_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_08_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 9",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Tumor_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_09_PDAC-Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 10",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Tumor_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_10_PDAC-Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 11",
"Factors":{"Sample type":"Healthy_Mouse_Pancreas_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_11_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 12",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Tumor_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_12_PDAC-Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 13",
"Factors":{"Sample type":"Healthy_Mouse_Pancreas_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_13_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Tissue 14",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Tumor_Tissue"},
"Additional sample data":{"RAW_FILE_NAME":"20211113_QE1_JAS_Job2416_Tissue_14_PDAC-Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 1",
"Factors":{"Sample type":"Healthy_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_15_Plasma-01_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 2",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_16_Plasma-02_PDAC Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 3",
"Factors":{"Sample type":"Healthy_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_17_Plasma-03_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 4",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_18_Plasma-04_PDAC Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 5",
"Factors":{"Sample type":"Healthy_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_19_Plasma-05_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 6",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_20_Plasma-06_PDAC Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 7",
"Factors":{"Sample type":"Healthy_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_21_Plasma-07_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 8",
"Factors":{"Sample type":"Healthy_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_22_Plasma-08_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 9",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_23_Plasma-09_PDAC Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 10",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_24_Plasma-10_PDAC Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 11",
"Factors":{"Sample type":"Healthy_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_25_Plasma-11_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 12",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_26_Plasma-12_PDAC Tumor.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 13",
"Factors":{"Sample type":"Healthy_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_27_Plasma-13_Pancreas.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Plasma 14",
"Factors":{"Sample type":"Tumor_Bearing_Mouse_Plasma"},
"Additional sample data":{"RAW_FILE_NAME":"20211105_QE2_JAS_Job2416_28_Plasma-14_PDAC Tumor.raw"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"~100uL of blood were be obtained by submandibular sampling as described previously (Parasuraman et al., 2010) and centrifuged at 845 x g for 10 minutes at 4°C to separate plasma. Plasma was frozen in liquid nitrogen and stored at -80°C until further analysis animals. Mice were then immediately euthanized and tumor or pancreas from each animal was then harvested and immediately snap frozen using a BioSqueezer (BioSpec) cooled with liquid nitrogen and stored at -80°F until further analysis.","SAMPLE_TYPE":"Tumor, Pancreas & Plasma"},

"TREATMENT":{"TREATMENT_SUMMARY":"Orthotopic tumors were implanted in C57BL6J mice at 8-12 weeks of age. 4 weeks after induction tumor-bearing mice and healthy littermate controls were treated with 15N2-glutamine (Cambridge Isotope Laboratory #NLM-1328-PK) dissolved in sterile phosphate buffered saline at 7.2mg/animal by tail vein injection as previously described (Lane et al., 2015). Briefly, animals were dosed three times at 15-minute intervals."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Cryogenically frozen tumor pieces were ground to a fine homogenous powder with a liquid nitrogen cooled mortar and pestle. ~30mg of tissue powder was weighed into sample tubes, and metabolites were extracted with 600µL HPLC grade methanol, 300µL HPLC grade water, and 400µL chloroform. Samples were vortexed for 10min at 4°C, centrifuged 21,000xg at 4°C for 10 min. 400µL of the aqueous top layer was removed into a new tube and dried under nitrogen. Dried tumor extracts were resuspended in 100µL HPLC grade water and LC-MS analysis was performed as described before(Sullivan et al., 2019b, 2019a). For plasma samples, we extracted polar metabolites from 5µL of sample using 45µL of a 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix. Samples in extraction mix were vortexed for 10 min at 4°C and centrifugated at 15,000x rpm for 10 min at 4°C to pellet insoluble material. 20µL of the soluble polar metabolite supernatant was moved to sample vials for analysis by LC-MS."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um)","SOLVENT_A":"20 mM ammonium carbonate, 0.1% ammonium hydroxide","SOLVENT_B":"acetonitrile","FLOW_GRADIENT":"linear gradient from 80% to 20% B; 20–20.5 min: linear gradient from 20% to 80% B; 20.5–28 min: hold at 80% B","FLOW_RATE":"0.150 mL/min","COLUMN_TEMPERATURE":"25"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"XCalibur 965 2.2 software (Thermo 966 Fisher Scientific) was used identification and relative quantification for metabolites. Natural abundance correction was performed using the IsoCor (Millard et al., 2019)."},

"MS_METABOLITE_DATA":{
"Units":"Fractional labelling",

"Data":[{"Metabolite":"M+0 Arginine","Plasma 1":"0.789967849","Plasma 3":"0.759514144","Plasma 5":"0.754847488","Plasma 7":"0.780332898","Plasma 8":"0.767656111","Plasma 11":"0.8031745","Plasma 13":"0.768640947","Plasma 2":"0.73812794","Plasma 4":"0.772541033","Plasma 6":"0.777178846","Plasma 9":"0.778667869","Plasma 10":"0.787473232","Plasma 12":"0.780095552","Plasma 14":"0.798969929","Tissue 1":"0.799444678","Tissue 3":"0.636254645","Tissue 5":"0.803379777","Tissue 7":"0.673511201","Tissue 8":"0.835144716","Tissue 11":"0.821035909","Tissue 13":"0.68589067","Tissue 2":"0.564968299","Tissue 4":"0.79738914","Tissue 6":"0.588690522","Tissue 9":"0.804261216","Tissue 10":"0.600258051","Tissue 12":"0.811388044","Tissue 14":"0.66625468"},{"Metabolite":"M+1 Arginine","Plasma 1":"0.11951694","Plasma 3":"0.140191212","Plasma 5":"0.136809675","Plasma 7":"0.118930742","Plasma 8":"0.11994946","Plasma 11":"0.124884204","Plasma 13":"0.141773449","Plasma 2":"0.135041515","Plasma 4":"0.131765882","Plasma 6":"0.126744492","Plasma 9":"0.119985569","Plasma 10":"0.119011855","Plasma 12":"0.122444301","Plasma 14":"0.131807945","Tissue 1":"0.124589148","Tissue 3":"0.201500859","Tissue 5":"0.125200409","Tissue 7":"0.173625874","Tissue 8":"0.109195741","Tissue 11":"0.120601839","Tissue 13":"0.203566763","Tissue 2":"0.219537957","Tissue 4":"0.12797592","Tissue 6":"0.220748641","Tissue 9":"0.125797001","Tissue 10":"0.219712695","Tissue 12":"0.122423622","Tissue 14":"0.214692055"},{"Metabolite":"M+2 Arginine","Plasma 1":"0.048100708","Plasma 3":"0.054878614","Plasma 5":"0.058375686","Plasma 7":"0.05342794","Plasma 8":"0.064838002","Plasma 11":"0.043882335","Plasma 13":"0.054232602","Plasma 2":"0.067404952","Plasma 4":"0.054262069","Plasma 6":"0.054649484","Plasma 9":"0.052252924","Plasma 10":"0.053496321","Plasma 12":"0.053630222","Plasma 14":"0.039998725","Tissue 1":"0.047945066","Tissue 3":"0.102750548","Tissue 5":"0.045705661","Tissue 7":"0.093744392","Tissue 8":"0.03564315","Tissue 11":"0.039379339","Tissue 13":"0.079790331","Tissue 2":"0.133175184","Tissue 4":"0.048427486","Tissue 6":"0.120761801","Tissue 9":"0.044937291","Tissue 10":"0.120323996","Tissue 12":"0.040446242","Tissue 14":"0.085823298"},{"Metabolite":"M+3 Arginine","Plasma 1":"0.033635296","Plasma 3":"0.035537227","Plasma 5":"0.041119539","Plasma 7":"0.037470461","Plasma 8":"0.036478175","Plasma 11":"0.023157838","Plasma 13":"0.028890145","Plasma 2":"0.041840923","Plasma 4":"0.034181522","Plasma 6":"0.033849501","Plasma 9":"0.03889497","Plasma 10":"0.030378922","Plasma 12":"0.035441619","Plasma 14":"0.022664198","Tissue 1":"0.022314946","Tissue 3":"0.046984766","Tissue 5":"0.020577175","Tissue 7":"0.045309257","Tissue 8":"0.01458863","Tissue 11":"0.015459835","Tissue 13":"0.025400228","Tissue 2":"0.064401222","Tissue 4":"0.021165912","Tissue 6":"0.056383472","Tissue 9":"0.019894621","Tissue 10":"0.048604014","Tissue 12":"0.019622375","Tissue 14":"0.027037486"},{"Metabolite":"M+4 Arginine","Plasma 1":"0.008779207","Plasma 3":"0.009878804","Plasma 5":"0.008847612","Plasma 7":"0.009837958","Plasma 8":"0.011078252","Plasma 11":"0.004901123","Plasma 13":"0.006462857","Plasma 2":"0.017584671","Plasma 4":"0.007249493","Plasma 6":"0.007577677","Plasma 9":"0.010198668","Plasma 10":"0.009639671","Plasma 12":"0.008388307","Plasma 14":"0.006559204","Tissue 1":"0.005706162","Tissue 3":"0.012509182","Tissue 5":"0.005136978","Tissue 7":"0.013809275","Tissue 8":"0.005427763","Tissue 11":"0.003523077","Tissue 13":"0.005352008","Tissue 2":"0.017917339","Tissue 4":"0.005041541","Tissue 6":"0.013415564","Tissue 9":"0.005109871","Tissue 10":"0.011101244","Tissue 12":"0.006119717","Tissue 14":"0.006192481"},{"Metabolite":"M+0 Citrulline","Plasma 1":"0.37941889","Plasma 3":"0.429350099","Plasma 5":"0.375515469","Plasma 7":"0.418116073","Plasma 8":"0.388021173","Plasma 11":"0.438334284","Plasma 13":"0.527492967","Plasma 2":"0.401538349","Plasma 4":"0.407074619","Plasma 6":"0.425333001","Plasma 9":"0.373227092","Plasma 10":"0.414955356","Plasma 12":"0.422508196","Plasma 14":"0.532412527","Tissue 1":"0.640300904","Tissue 3":"0.49594539","Tissue 5":"0.718289545","Tissue 7":"0.456749015","Tissue 8":"0.781367876","Tissue 11":"0.659346753","Tissue 13":"0.640868795","Tissue 2":"0.419775172","Tissue 4":"0.623899336","Tissue 6":"0.452226748","Tissue 9":"0.674994433","Tissue 10":"0.44089311","Tissue 12":"0.682754438","Tissue 14":"0.628977288"},{"Metabolite":"M+1 Citrulline","Plasma 1":"0.342815565","Plasma 3":"0.323194312","Plasma 5":"0.344130166","Plasma 7":"0.321963598","Plasma 8":"0.337582075","Plasma 11":"0.348582583","Plasma 13":"0.314334082","Plasma 2":"0.328540606","Plasma 4":"0.349239237","Plasma 6":"0.326914036","Plasma 9":"0.353683923","Plasma 10":"0.35298487","Plasma 12":"0.329176194","Plasma 14":"0.314784308","Tissue 1":"0.215417518","Tissue 3":"0.270403807","Tissue 5":"0.181453246","Tissue 7":"0.277997371","Tissue 8":"0.153773841","Tissue 11":"0.220342215","Tissue 13":"0.249275195","Tissue 2":"0.304200904","Tissue 4":"0.230162479","Tissue 6":"0.299485153","Tissue 9":"0.201046442","Tissue 10":"0.328353559","Tissue 12":"0.19364112","Tissue 14":"0.263242319"},{"Metabolite":"M+2 Citrulline","Plasma 1":"0.208378656","Plasma 3":"0.186468393","Plasma 5":"0.210339241","Plasma 7":"0.192301206","Plasma 8":"0.205307956","Plasma 11":"0.170389597","Plasma 13":"0.128362074","Plasma 2":"0.203542692","Plasma 4":"0.188175561","Plasma 6":"0.189191568","Plasma 9":"0.207662768","Plasma 10":"0.184360194","Plasma 12":"0.187520914","Plasma 14":"0.126060707","Tissue 1":"0.109199884","Tissue 3":"0.166747061","Tissue 5":"0.0797481","Tissue 7":"0.185354868","Tissue 8":"0.05144813","Tissue 11":"0.10204325","Tissue 13":"0.089848918","Tissue 2":"0.199505439","Tissue 4":"0.122435171","Tissue 6":"0.177743143","Tissue 9":"0.09400688","Tissue 10":"0.180594181","Tissue 12":"0.096943079","Tissue 14":"0.092627675"},{"Metabolite":"M+3 Citrulline","Plasma 1":"0.06938689","Plasma 3":"0.060987196","Plasma 5":"0.070015123","Plasma 7":"0.067619123","Plasma 8":"0.069088797","Plasma 11":"0.042693536","Plasma 13":"0.029810877","Plasma 2":"0.066378352","Plasma 4":"0.055510583","Plasma 6":"0.058561395","Plasma 9":"0.065426218","Plasma 10":"0.04769958","Plasma 12":"0.060794696","Plasma 14":"0.026742458","Tissue 1":"0.035081694","Tissue 3":"0.066903742","Tissue 5":"0.020509109","Tissue 7":"0.079898745","Tissue 8":"0.013410154","Tissue 11":"0.018267782","Tissue 13":"0.020007091","Tissue 2":"0.076518485","Tissue 4":"0.023503013","Tissue 6":"0.070544956","Tissue 9":"0.029952245","Tissue 10":"0.050159151","Tissue 12":"0.026661363","Tissue 14":"0.015152718"},{"Metabolite":"M+0 Glutamine","Plasma 1":"0.308184681","Plasma 3":"0.301925913","Plasma 5":"0.282815347","Plasma 7":"0.30941817","Plasma 8":"0.31651427","Plasma 11":"0.355424553","Plasma 13":"0.397315697","Plasma 2":"0.270679429","Plasma 4":"0.336774245","Plasma 6":"0.313074149","Plasma 9":"0.312081111","Plasma 10":"0.335007771","Plasma 12":"0.322040484","Plasma 14":"0.395499898","Tissue 1":"0.353570085","Tissue 3":"0.324758044","Tissue 5":"0.358195344","Tissue 7":"0.358639284","Tissue 8":"0.43572223","Tissue 11":"0.370840266","Tissue 13":"0.427133614","Tissue 2":"0.268598187","Tissue 4":"0.356779562","Tissue 6":"0.331527326","Tissue 9":"0.336508915","Tissue 10":"0.344536496","Tissue 12":"0.386610208","Tissue 14":"0.50024661"},{"Metabolite":"M+1 Glutamine","Plasma 1":"0.358930559","Plasma 3":"0.308622332","Plasma 5":"0.346059096","Plasma 7":"0.315479022","Plasma 8":"0.349389481","Plasma 11":"0.368419013","Plasma 13":"0.328990713","Plasma 2":"0.311507479","Plasma 4":"0.33738744","Plasma 6":"0.315295417","Plasma 9":"0.374693843","Plasma 10":"0.331670974","Plasma 12":"0.358063257","Plasma 14":"0.34261402","Tissue 1":"0.240775502","Tissue 3":"0.277591221","Tissue 5":"0.218929029","Tissue 7":"0.272520668","Tissue 8":"0.210448065","Tissue 11":"0.268704245","Tissue 13":"0.277262156","Tissue 2":"0.267972479","Tissue 4":"0.237479445","Tissue 6":"0.281870078","Tissue 9":"0.257737126","Tissue 10":"0.322185521","Tissue 12":"0.215477985","Tissue 14":"0.293946367"},{"Metabolite":"M+2 Glutamine","Plasma 1":"0.33288476","Plasma 3":"0.389451755","Plasma 5":"0.371125558","Plasma 7":"0.375102808","Plasma 8":"0.334096249","Plasma 11":"0.276156434","Plasma 13":"0.27369359","Plasma 2":"0.417813092","Plasma 4":"0.325838315","Plasma 6":"0.371630435","Plasma 9":"0.313225046","Plasma 10":"0.333321256","Plasma 12":"0.319896259","Plasma 14":"0.261886082","Tissue 1":"0.405654413","Tissue 3":"0.397650735","Tissue 5":"0.422875627","Tissue 7":"0.368840048","Tissue 8":"0.353829705","Tissue 11":"0.360455489","Tissue 13":"0.295604231","Tissue 2":"0.463429334","Tissue 4":"0.405740993","Tissue 6":"0.386602597","Tissue 9":"0.405753959","Tissue 10":"0.333277983","Tissue 12":"0.397911807","Tissue 14":"0.205807023"},{"Metabolite":"M+0 Ornithine","Plasma 1":"0","Plasma 3":"0.498795491","Plasma 5":"0.589386087","Plasma 7":"0","Plasma 8":"0","Plasma 11":"0.200538232","Plasma 13":"0","Plasma 2":"0.485728265","Plasma 4":"0.436332107","Plasma 6":"0.682343591","Plasma 9":"0","Plasma 10":"0.72953526","Plasma 12":"0.436000816","Plasma 14":"0.568384231","Tissue 1":"0.863108358","Tissue 3":"0.845085516","Tissue 5":"0.879467915","Tissue 7":"0.849854594","Tissue 8":"0.89353327","Tissue 11":"0.886374642","Tissue 13":"0.857813143","Tissue 2":"0.80090085","Tissue 4":"0.857365429","Tissue 6":"0.811876751","Tissue 9":"0.864706314","Tissue 10":"0.8220455","Tissue 12":"0.885656357","Tissue 14":"0.879170879"},{"Metabolite":"M+1 Ornithine","Plasma 1":"0.683033885","Plasma 3":"0","Plasma 5":"0.186128806","Plasma 7":"0","Plasma 8":"0.595465756","Plasma 11":"0.413747945","Plasma 13":"0.439693493","Plasma 2":"0.336963561","Plasma 4":"0","Plasma 6":"0.152286005","Plasma 9":"0","Plasma 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}

}