{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002549","ANALYSIS_ID":"AN004197","VERSION":"1","CREATED_ON":"April 7, 2023, 1:28 pm"},

"PROJECT":{"PROJECT_TITLE":"Lipidomic analysis of serum from mice with Toxoplasma gondii infection","PROJECT_SUMMARY":"Cachexia is a life-threatening disease characterized by chronic, inflammatory muscle wasting and systemic metabolic impairment. Despite its high prevalence, there are no efficacious therapies for cachexia. Mice chronically infected with the protozoan parasite Toxoplasma gondii represent a novel animal model recapitulating the chronic kinetics of cachexia. To understand how perturbations to metabolic tissue homeostasis influence circulating metabolite availability we used mass spectrometry analysis. Despite the significant reduction in circulating triacylglycerides, nonesterified fatty acids, and glycerol, sphingolipid long-chain bases and a subset of phosphatidylcholines (PCs) were significantly increased in the sera of mice with T. gondii infection-induced cachexia. In addition, the TCA cycle intermediates -ketoglutarate, 2- hydroxyglutarate, succinate, fumarate, and malate were highly depleted in cachectic mouse sera. Sphingolipids and their de novo synthesis precursors PCs are the major components of the mitochondrial membrane and regulate mitochondrial function consistent with a causal relationship in the energy imbalance driving T. gondii-induced chronic cachexia.","INSTITUTE":"University of Virginia","LAST_NAME":"Feng","FIRST_NAME":"Tzu-Yu","ADDRESS":"345 Crispell Dr.","EMAIL":"ttf4ye@virginia.edu","PHONE":"702-217-4454"},

"STUDY":{"STUDY_TITLE":"Lipidomic analysis of serum from mice with Toxoplasma gondii infection","STUDY_SUMMARY":"Cachexia is a life-threatening disease characterized by chronic, inflammatory muscle wasting and systemic metabolic impairment. Despite its high prevalence, there are no efficacious therapies for cachexia. Mice chronically infected with the protozoan parasite Toxoplasma gondii represent a novel animal model recapitulating the chronic kinetics of cachexia. To understand how perturbations to metabolic tissue homeostasis influence circulating metabolite availability we used mass spectrometry analysis. Despite the significant reduction in circulating triacylglycerides, nonesterified fatty acids, and glycerol, sphingolipid long-chain bases and a subset of phosphatidylcholines (PCs) were significantly increased in the sera of mice with T. gondii infection-induced cachexia. In addition, the TCA cycle intermediates -ketoglutarate, 2- hydroxyglutarate, succinate, fumarate, and malate were highly depleted in cachectic mouse sera. Sphingolipids and their de novo synthesis precursors PCs are the major components of the mitochondrial membrane and regulate mitochondrial function consistent with a causal relationship in the energy imbalance driving T. gondii-induced chronic cachexia.","INSTITUTE":"University of Virginia","LAST_NAME":"Feng","FIRST_NAME":"Tzu-Yu","ADDRESS":"345 Crispell Dr.","EMAIL":"ttf4ye@virginia.edu","PHONE":"702-217-4454"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Uninfected-1",
"Factors":{"Factor":"Uninfected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_27"}
},
{
"Subject ID":"-",
"Sample ID":"Uninfected-2",
"Factors":{"Factor":"Uninfected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_28"}
},
{
"Subject ID":"-",
"Sample ID":"Uninfected-3",
"Factors":{"Factor":"Uninfected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_29"}
},
{
"Subject ID":"-",
"Sample ID":"Uninfected-4",
"Factors":{"Factor":"Uninfected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_30"}
},
{
"Subject ID":"-",
"Sample ID":"Uninfected-5",
"Factors":{"Factor":"Uninfected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_31"}
},
{
"Subject ID":"-",
"Sample ID":"Uninfected-6",
"Factors":{"Factor":"Uninfected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_32"}
},
{
"Subject ID":"-",
"Sample ID":"Uninfected-7",
"Factors":{"Factor":"Uninfected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_33"}
},
{
"Subject ID":"-",
"Sample ID":"Uninfected-8",
"Factors":{"Factor":"Uninfected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_34"}
},
{
"Subject ID":"-",
"Sample ID":"Uninfected-9",
"Factors":{"Factor":"Uninfected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_35"}
},
{
"Subject ID":"-",
"Sample ID":"Toxoplasma infected-1",
"Factors":{"Factor":"Infected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_36"}
},
{
"Subject ID":"-",
"Sample ID":"Toxoplasma infected-2",
"Factors":{"Factor":"Infected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_37"}
},
{
"Subject ID":"-",
"Sample ID":"Toxoplasma infected-3",
"Factors":{"Factor":"Infected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_38"}
},
{
"Subject ID":"-",
"Sample ID":"Toxoplasma infected-4",
"Factors":{"Factor":"Infected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_39"}
},
{
"Subject ID":"-",
"Sample ID":"Toxoplasma infected-5",
"Factors":{"Factor":"Infected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_40"}
},
{
"Subject ID":"-",
"Sample ID":"Toxoplasma infected-6",
"Factors":{"Factor":"Infected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_41"}
},
{
"Subject ID":"-",
"Sample ID":"Toxoplasma infected-7",
"Factors":{"Factor":"Infected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_43"}
},
{
"Subject ID":"-",
"Sample ID":"Toxoplasma infected-8",
"Factors":{"Factor":"Infected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_44"}
},
{
"Subject ID":"-",
"Sample ID":"Toxoplasma infected-9",
"Factors":{"Factor":"Infected"},
"Additional sample data":{"RAW_FILE_NAME":"2020_10_28_Ewald_Plasma_SLs_45"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"At 7 weeks post-infection, isoflurane-anesthetized mice were retro-orbitally bled and sera was flash frozen and sent to the National Institute of Health (NIH) West Coast Metabolomics Center (UC Davis) for untargeted mass spectrometry analysisusing the primary metabolism assay (ALEXCIS GCTOF-MS) or the complex lipids (CSH-QTOF MS) assay. Detected meatbolites were identified based on retention time and mass spectra from MassBank of North America, curated by the NIH West Coast Metabolomics Center , and reported as raw peak heights (Table S1 and S3). The raw peak heights from each analytical platform were normalized to the average peak heights of the identified metabolites in uninfected group. The resulting data were analyzed for fold-change and multiple unpaired t-test and visualized using volcano plots to identify the differential expression of metabolites in response to T. gondii-induced cachexia.","SAMPLE_TYPE":"Blood (serum)"},

"TREATMENT":{"TREATMENT_SUMMARY":"To generate cysts for infection, 8-10 week female CBA/J mice were infected with 10 Me49 bradyzoite cysts by intraperitoneal injection. 4–8 weeks following infection, mice were euthanized by CO2 inhalation and cysts were harvest from brains homogenate passed through a 70 μm filter. Homegenate was washed 3 times in PBS, stained with dolichos biflorus agglutinin conjugated to FITC (Vector labs) at a 1:500 dilution. The number of cysts were determined by counting FITCpositive cysts at 20x magnification using an EVOS FL imaging system (Thermo Fisher). For experimental infections 10–14-week-old male C57BL/6 mice were infected with 10 Me49 bradyzoite cysts by intraperitoneal infection resuspended in 200 Μl PBS per mouse using a 5G 5/8” tuberculin syringe. Prior to infection, mice were cross-housed on dirty, wood chip bedding for two weeks to normalize commensal microbiota and limit the effect of eating corn husk bedding on dietary metabolites. At experimental endpoints, mice were fasted for 4 hours and isoflurane anaesthetized to isolate sera via retro-orbital bleed and/or euthanized by CO2 asphyxiation to harvest tissues for weighing and histological analysis."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"At 7 weeks post-infection, isoflurane-anesthetized mice were retro-orbitally bled and sera was flash frozen and sent to the National Institute of Health (NIH) West Coast Metabolomics Center (UC Davis) for untargeted mass spectrometry analysisusing the primary metabolism assay (ALEXCIS GCTOF-MS) or the complex lipids (CSH-QTOF MS) assay. Detected meatbolites were identified based on retention time and mass spectra from MassBank of North America, curated by the NIH West Coast Metabolomics Center , and reported as raw peak heights (Table S1 and S3). The raw peak heights from each analytical platform were normalized to the average peak heights of the identified metabolites in uninfected group. The resulting data were analyzed for fold-change and multiple unpaired t-test and visualized using volcano plots to identify the differential expression of metabolites in response to T. gondii-induced cachexia."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Low pH polar","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)","SOLVENT_A":"60% water/40% acetonitrile","SOLVENT_B":"90% isopropanol/10% methanol","FLOW_GRADIENT":"30% B for 0.5min, a linear gradient to 40% B for 3min, 60% B over 3min, then 85% B for 4.5min","FLOW_RATE":"0.4mL/min","COLUMN_TEMPERATURE":"65"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Waters Xevo-TQ-S","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"Other","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"Lipid extraction and analysis for targeted sphingolipid analysis was done using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS)69. Lipids were extracted from sera using an azeotrophic mix of isopropanol:water:ethyl acetate (3:1:6; v:v:v). Internal standards (10 pmol of d17 long-chain bases and C12 acylated sphingolipids) were added to samples at the onset of the extraction procedure. Extracts were separated on a Waters Iclass Acquity UPLC chromatography system. Mobile phases were (A) 60:40 water:acetonitrile and (B) 90:10 isopropanol:methanol with both mobile phases containing 5 mM ammonium formate and 0.1% formic acid. A Waters C18 CSH 2.1 mm ID × 10 cm column maintained at 65°C was used for the separation of the sphingoid bases, 1-phosphates, and acylated sphingolipids. The eluate was analyzed with an inline Waters TQ-S mass spectrometer using multiple reaction monitoring."},

"MS_METABOLITE_DATA":{
"Units":"pmol/mL of plasma",

"Data":[{"Metabolite":"Sph","Toxoplasma infected-1":"123.96","Toxoplasma infected-2":"130.23","Toxoplasma infected-3":"82.67","Toxoplasma infected-4":"41.34","Toxoplasma infected-5":"122.48","Toxoplasma infected-6":"58.61","Toxoplasma infected-7":"43.85","Toxoplasma infected-8":"49.39","Toxoplasma infected-9":"29.44","Uninfected-1":"45.88","Uninfected-2":"26.74","Uninfected-3":"72.20","Uninfected-4":"62.20","Uninfected-5":"61.32","Uninfected-6":"44.73","Uninfected-7":"65.14","Uninfected-8":"58.13","Uninfected-9":"36.70"},{"Metabolite":"dhSph","Toxoplasma infected-1":"161.42","Toxoplasma infected-2":"155.47","Toxoplasma infected-3":"94.55","Toxoplasma infected-4":"48.12","Toxoplasma infected-5":"147.86","Toxoplasma infected-6":"66.76","Toxoplasma infected-7":"46.79","Toxoplasma infected-8":"62.52","Toxoplasma infected-9":"29.68","Uninfected-1":"51.82","Uninfected-2":"38.13","Uninfected-3":"81.30","Uninfected-4":"76.01","Uninfected-5":"72.50","Uninfected-6":"55.01","Uninfected-7":"70.28","Uninfected-8":"75.09","Uninfected-9":"38.59"},{"Metabolite":"S1P","Toxoplasma infected-1":"4048.32","Toxoplasma infected-2":"4207.97","Toxoplasma infected-3":"3880.85","Toxoplasma infected-4":"3623.65","Toxoplasma infected-5":"3938.66","Toxoplasma infected-6":"4375.00","Toxoplasma infected-7":"4082.47","Toxoplasma infected-8":"3496.77","Toxoplasma infected-9":"2365.45","Uninfected-1":"2782.51","Uninfected-2":"3042.28","Uninfected-3":"3332.13","Uninfected-4":"2954.61","Uninfected-5":"2886.61","Uninfected-6":"3346.80","Uninfected-7":"3005.36","Uninfected-8":"3553.26","Uninfected-9":"3279.96"},{"Metabolite":"dhS1P","Toxoplasma infected-1":"2038.09","Toxoplasma infected-2":"1708.26","Toxoplasma infected-3":"1664.26","Toxoplasma infected-4":"1124.11","Toxoplasma infected-5":"1771.59","Toxoplasma infected-6":"1726.96","Toxoplasma infected-7":"1651.66","Toxoplasma infected-8":"1590.24","Toxoplasma infected-9":"1030.52","Uninfected-1":"1076.49","Uninfected-2":"1118.87","Uninfected-3":"1366.54","Uninfected-4":"1093.12","Uninfected-5":"1000.26","Uninfected-6":"916.17","Uninfected-7":"1095.88","Uninfected-8":"1327.46","Uninfected-9":"1199.91"},{"Metabolite":"HexSph","Toxoplasma infected-1":"1.958","Toxoplasma infected-2":"1.435","Toxoplasma infected-3":"1.793","Toxoplasma infected-4":"0.641","Toxoplasma infected-5":"1.350","Toxoplasma infected-6":"0.891","Toxoplasma infected-7":"1.498","Toxoplasma infected-8":"1.358","Toxoplasma infected-9":"1.390","Uninfected-1":"1.253","Uninfected-2":"1.329","Uninfected-3":"1.317","Uninfected-4":"1.837","Uninfected-5":"0.965","Uninfected-6":"1.470","Uninfected-7":"2.056","Uninfected-8":"1.597","Uninfected-9":"1.338"},{"Metabolite":"LysoSM","Toxoplasma infected-1":"1.757","Toxoplasma infected-2":"1.652","Toxoplasma infected-3":"2.143","Toxoplasma infected-4":"2.631","Toxoplasma infected-5":"1.516","Toxoplasma infected-6":"1.820","Toxoplasma infected-7":"1.879","Toxoplasma infected-8":"2.231","Toxoplasma infected-9":"2.460","Uninfected-1":"2.338","Uninfected-2":"1.800","Uninfected-3":"1.515","Uninfected-4":"1.554","Uninfected-5":"2.036","Uninfected-6":"1.385","Uninfected-7":"1.222","Uninfected-8":"1.881","Uninfected-9":"1.637"},{"Metabolite":"Cer C16","Toxoplasma infected-1":"621.70","Toxoplasma infected-2":"749.05","Toxoplasma infected-3":"691.52","Toxoplasma infected-4":"626.16","Toxoplasma infected-5":"562.85","Toxoplasma infected-6":"613.96","Toxoplasma infected-7":"691.86","Toxoplasma infected-8":"590.92","Toxoplasma infected-9":"474.33","Uninfected-1":"684.81","Uninfected-2":"516.03","Uninfected-3":"729.22","Uninfected-4":"576.47","Uninfected-5":"503.40","Uninfected-6":"580.21","Uninfected-7":"619.37","Uninfected-8":"530.95","Uninfected-9":"472.28"},{"Metabolite":"Cer C18","Toxoplasma infected-1":"26.14","Toxoplasma infected-2":"58.59","Toxoplasma infected-3":"55.54","Toxoplasma infected-4":"26.16","Toxoplasma infected-5":"38.84","Toxoplasma infected-6":"48.85","Toxoplasma infected-7":"41.64","Toxoplasma infected-8":"36.68","Toxoplasma infected-9":"27.60","Uninfected-1":"39.39","Uninfected-2":"19.62","Uninfected-3":"44.46","Uninfected-4":"18.01","Uninfected-5":"26.21","Uninfected-6":"23.03","Uninfected-7":"20.38","Uninfected-8":"24.17","Uninfected-9":"13.26"},{"Metabolite":"Cer C20","Toxoplasma infected-1":"191.90","Toxoplasma infected-2":"156.03","Toxoplasma infected-3":"218.06","Toxoplasma infected-4":"187.32","Toxoplasma infected-5":"181.56","Toxoplasma infected-6":"165.91","Toxoplasma infected-7":"251.39","Toxoplasma infected-8":"108.51","Toxoplasma infected-9":"137.62","Uninfected-1":"250.65","Uninfected-2":"149.62","Uninfected-3":"282.84","Uninfected-4":"186.08","Uninfected-5":"186.61","Uninfected-6":"127.74","Uninfected-7":"214.04","Uninfected-8":"151.56","Uninfected-9":"131.17"},{"Metabolite":"Cer C22","Toxoplasma infected-1":"1895.44","Toxoplasma infected-2":"1535.59","Toxoplasma infected-3":"2107.59","Toxoplasma infected-4":"2413.10","Toxoplasma infected-5":"1628.54","Toxoplasma infected-6":"1186.32","Toxoplasma infected-7":"1704.46","Toxoplasma infected-8":"999.38","Toxoplasma infected-9":"1299.64","Uninfected-1":"1931.80","Uninfected-2":"2323.01","Uninfected-3":"2602.25","Uninfected-4":"2325.24","Uninfected-5":"2537.86","Uninfected-6":"2112.33","Uninfected-7":"3205.89","Uninfected-8":"2172.93","Uninfected-9":"2021.25"},{"Metabolite":"Cer C22:1","Toxoplasma infected-1":"147.60","Toxoplasma infected-2":"128.58","Toxoplasma infected-3":"150.71","Toxoplasma infected-4":"175.61","Toxoplasma infected-5":"71.56","Toxoplasma infected-6":"67.23","Toxoplasma infected-7":"88.83","Toxoplasma infected-8":"59.99","Toxoplasma infected-9":"84.71","Uninfected-1":"327.82","Uninfected-2":"152.14","Uninfected-3":"617.59","Uninfected-4":"171.85","Uninfected-5":"195.49","Uninfected-6":"76.28","Uninfected-7":"231.47","Uninfected-8":"166.48","Uninfected-9":"109.83"},{"Metabolite":"Cer C24","Toxoplasma infected-1":"3179.05","Toxoplasma infected-2":"3045.30","Toxoplasma infected-3":"3839.18","Toxoplasma infected-4":"3557.35","Toxoplasma infected-5":"3194.36","Toxoplasma infected-6":"2799.46","Toxoplasma infected-7":"2862.87","Toxoplasma infected-8":"2519.44","Toxoplasma infected-9":"2601.32","Uninfected-1":"2745.23","Uninfected-2":"4219.86","Uninfected-3":"3361.08","Uninfected-4":"4663.96","Uninfected-5":"3848.05","Uninfected-6":"4043.47","Uninfected-7":"5390.13","Uninfected-8":"4708.62","Uninfected-9":"3447.87"},{"Metabolite":"Cer C24:1","Toxoplasma infected-1":"3660.55","Toxoplasma infected-2":"2845.13","Toxoplasma infected-3":"3791.65","Toxoplasma infected-4":"4252.38","Toxoplasma infected-5":"3301.90","Toxoplasma infected-6":"2722.86","Toxoplasma infected-7":"2426.92","Toxoplasma infected-8":"3235.18","Toxoplasma infected-9":"2545.98","Uninfected-1":"4464.09","Uninfected-2":"4148.72","Uninfected-3":"5456.69","Uninfected-4":"5072.10","Uninfected-5":"4075.28","Uninfected-6":"2883.68","Uninfected-7":"4903.33","Uninfected-8":"4340.43","Uninfected-9":"2883.49"},{"Metabolite":"dhC16","Toxoplasma infected-1":"24.09","Toxoplasma infected-2":"66.88","Toxoplasma infected-3":"27.78","Toxoplasma infected-4":"62.65","Toxoplasma infected-5":"29.80","Toxoplasma infected-6":"21.35","Toxoplasma infected-7":"24.71","Toxoplasma infected-8":"14.96","Toxoplasma infected-9":"14.73","Uninfected-1":"20.84","Uninfected-2":"36.94","Uninfected-3":"25.12","Uninfected-4":"32.95","Uninfected-5":"40.96","Uninfected-6":"33.04","Uninfected-7":"38.46","Uninfected-8":"18.11","Uninfected-9":"55.33"},{"Metabolite":"dhC18","Toxoplasma infected-1":"9.31","Toxoplasma infected-2":"13.70","Toxoplasma infected-3":"9.73","Toxoplasma infected-4":"12.91","Toxoplasma infected-5":"12.69","Toxoplasma infected-6":"9.23","Toxoplasma infected-7":"17.52","Toxoplasma infected-8":"6.44","Toxoplasma infected-9":"5.95","Uninfected-1":"14.71","Uninfected-2":"11.56","Uninfected-3":"10.22","Uninfected-4":"6.44","Uninfected-5":"10.00","Uninfected-6":"7.17","Uninfected-7":"10.42","Uninfected-8":"13.30","Uninfected-9":"12.50"},{"Metabolite":"dhC20","Toxoplasma infected-1":"12.99","Toxoplasma infected-2":"20.67","Toxoplasma infected-3":"11.89","Toxoplasma infected-4":"28.90","Toxoplasma infected-5":"18.18","Toxoplasma infected-6":"8.10","Toxoplasma infected-7":"17.56","Toxoplasma infected-8":"10.70","Toxoplasma infected-9":"8.57","Uninfected-1":"11.03","Uninfected-2":"38.75","Uninfected-3":"27.26","Uninfected-4":"15.35","Uninfected-5":"22.92","Uninfected-6":"17.19","Uninfected-7":"29.24","Uninfected-8":"13.53","Uninfected-9":"25.96"},{"Metabolite":"dhC22","Toxoplasma infected-1":"121.86","Toxoplasma infected-2":"286.41","Toxoplasma infected-3":"128.67","Toxoplasma infected-4":"395.65","Toxoplasma infected-5":"140.08","Toxoplasma infected-6":"76.75","Toxoplasma infected-7":"136.14","Toxoplasma infected-8":"71.34","Toxoplasma infected-9":"68.87","Uninfected-1":"158.16","Uninfected-2":"387.69","Uninfected-3":"221.81","Uninfected-4":"201.76","Uninfected-5":"327.00","Uninfected-6":"221.59","Uninfected-7":"343.73","Uninfected-8":"206.97","Uninfected-9":"338.06"},{"Metabolite":"dhC24","Toxoplasma infected-1":"152.78","Toxoplasma infected-2":"376.59","Toxoplasma infected-3":"154.97","Toxoplasma infected-4":"444.93","Toxoplasma infected-5":"208.03","Toxoplasma infected-6":"138.05","Toxoplasma infected-7":"240.42","Toxoplasma infected-8":"105.68","Toxoplasma infected-9":"108.56","Uninfected-1":"204.45","Uninfected-2":"459.55","Uninfected-3":"259.58","Uninfected-4":"285.56","Uninfected-5":"375.95","Uninfected-6":"255.97","Uninfected-7":"402.22","Uninfected-8":"298.20","Uninfected-9":"373.36"},{"Metabolite":"dhC24:1","Toxoplasma infected-1":"190.14","Toxoplasma infected-2":"314.20","Toxoplasma infected-3":"171.04","Toxoplasma infected-4":"459.04","Toxoplasma infected-5":"249.93","Toxoplasma infected-6":"130.54","Toxoplasma infected-7":"150.35","Toxoplasma infected-8":"149.31","Toxoplasma infected-9":"96.50","Uninfected-1":"225.97","Uninfected-2":"452.80","Uninfected-3":"332.94","Uninfected-4":"261.76","Uninfected-5":"383.02","Uninfected-6":"259.12","Uninfected-7":"425.98","Uninfected-8":"349.86","Uninfected-9":"310.86"},{"Metabolite":"Hex C16","Toxoplasma infected-1":"204.78","Toxoplasma infected-2":"254.67","Toxoplasma infected-3":"316.51","Toxoplasma infected-4":"123.12","Toxoplasma infected-5":"242.96","Toxoplasma infected-6":"173.23","Toxoplasma infected-7":"229.71","Toxoplasma infected-8":"354.68","Toxoplasma infected-9":"177.57","Uninfected-1":"144.35","Uninfected-2":"128.45","Uninfected-3":"144.30","Uninfected-4":"118.04","Uninfected-5":"119.47","Uninfected-6":"100.61","Uninfected-7":"118.98","Uninfected-8":"115.78","Uninfected-9":"121.01"},{"Metabolite":"Hex C18","Toxoplasma infected-1":"10.69","Toxoplasma infected-2":"12.80","Toxoplasma infected-3":"18.23","Toxoplasma infected-4":"18.81","Toxoplasma infected-5":"21.36","Toxoplasma infected-6":"19.32","Toxoplasma infected-7":"15.60","Toxoplasma infected-8":"12.85","Toxoplasma infected-9":"8.89","Uninfected-1":"18.18","Uninfected-2":"15.37","Uninfected-3":"23.37","Uninfected-4":"12.24","Uninfected-5":"12.52","Uninfected-6":"11.81","Uninfected-7":"13.93","Uninfected-8":"17.62","Uninfected-9":"18.42"},{"Metabolite":"Hex C20","Toxoplasma infected-1":"32.32","Toxoplasma infected-2":"37.86","Toxoplasma infected-3":"55.15","Toxoplasma infected-4":"53.32","Toxoplasma infected-5":"45.01","Toxoplasma infected-6":"36.39","Toxoplasma infected-7":"48.03","Toxoplasma infected-8":"38.15","Toxoplasma infected-9":"24.85","Uninfected-1":"75.93","Uninfected-2":"41.38","Uninfected-3":"84.82","Uninfected-4":"40.97","Uninfected-5":"50.60","Uninfected-6":"26.34","Uninfected-7":"40.81","Uninfected-8":"40.37","Uninfected-9":"52.23"},{"Metabolite":"Hex C22","Toxoplasma infected-1":"346.21","Toxoplasma infected-2":"392.64","Toxoplasma infected-3":"470.35","Toxoplasma infected-4":"604.17","Toxoplasma infected-5":"376.17","Toxoplasma infected-6":"283.19","Toxoplasma infected-7":"355.94","Toxoplasma infected-8":"336.25","Toxoplasma infected-9":"234.39","Uninfected-1":"667.33","Uninfected-2":"542.84","Uninfected-3":"731.59","Uninfected-4":"497.02","Uninfected-5":"584.14","Uninfected-6":"412.99","Uninfected-7":"581.91","Uninfected-8":"465.93","Uninfected-9":"617.12"},{"Metabolite":"Hex C22:1","Toxoplasma infected-1":"13.51","Toxoplasma infected-2":"16.64","Toxoplasma infected-3":"23.80","Toxoplasma infected-4":"29.38","Toxoplasma infected-5":"9.88","Toxoplasma infected-6":"6.55","Toxoplasma infected-7":"13.15","Toxoplasma infected-8":"17.35","Toxoplasma infected-9":"10.54","Uninfected-1":"83.43","Uninfected-2":"27.77","Uninfected-3":"99.10","Uninfected-4":"23.61","Uninfected-5":"28.75","Uninfected-6":"9.38","Uninfected-7":"25.89","Uninfected-8":"22.71","Uninfected-9":"19.18"},{"Metabolite":"Hex C24","Toxoplasma infected-1":"253.21","Toxoplasma infected-2":"299.46","Toxoplasma infected-3":"363.16","Toxoplasma infected-4":"309.31","Toxoplasma infected-5":"286.02","Toxoplasma infected-6":"232.53","Toxoplasma 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"Metabolites":[{"metabolite_name":"Sph"},{"metabolite_name":"dhSph"},{"metabolite_name":"S1P"},{"metabolite_name":"dhS1P"},{"metabolite_name":"HexSph"},{"metabolite_name":"LysoSM"},{"metabolite_name":"Cer C16"},{"metabolite_name":"Cer C18"},{"metabolite_name":"Cer C20"},{"metabolite_name":"Cer C22"},{"metabolite_name":"Cer C22:1"},{"metabolite_name":"Cer C24"},{"metabolite_name":"Cer C24:1"},{"metabolite_name":"dhC16"},{"metabolite_name":"dhC18"},{"metabolite_name":"dhC20"},{"metabolite_name":"dhC22"},{"metabolite_name":"dhC24"},{"metabolite_name":"dhC24:1"},{"metabolite_name":"Hex C16"},{"metabolite_name":"Hex C18"},{"metabolite_name":"Hex C20"},{"metabolite_name":"Hex C22"},{"metabolite_name":"Hex C22:1"},{"metabolite_name":"Hex C24"},{"metabolite_name":"Hex C24:1"},{"metabolite_name":"SpM C16"},{"metabolite_name":"SpM C18"},{"metabolite_name":"SpM C20"},{"metabolite_name":"SpM C20:1"},{"metabolite_name":"SpM C22"},{"metabolite_name":"SpM C22:1"},{"metabolite_name":"SpM C24"},{"metabolite_name":"SpM C24:1"}]
}

}