{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002725","ANALYSIS_ID":"AN004417","VERSION":"1","CREATED_ON":"June 6, 2023, 9:33 am"},

"PROJECT":{"PROJECT_TITLE":"Metabolic and Proteomic Changes in Sickle Cell Disease and B-thalassemia Mouse Splenic and Hepatic Macrophages and Peripheral Blood Mononuclear cells","PROJECT_SUMMARY":"Sickle cell disease and Beta-thalassemia represent hemoglobinopathies arising from dysfunctional or under produced beta-globin chains, respectively. In both diseases, red blood cell injury and anemia are the impetus for end organ injury. Because persistent erythrophagocytosis is a hallmark of these genetic maladies it is critical to understand how macrophage phenotype polarizations in tissue compartments can inform on disease progression. Murine models of sickle cell disease and Beta-thalassemia allow for a basic understanding of mechanisms and provide for translation to human disease. A multi-omics approach to understanding macrophage metabolism and protein changes in two murine models of beta-globinopathy was performed on peripheral blood mononuclear cells as well as spleen and liver macrophages isolated from Berkley sickle cell disease (Berk-ss) and heterozygous B1/B2 globin gene deletion (Hbbth3/+) mice. Results from these experiments revealed the metabolome and proteome of macrophages are polarized to a distinct phenotype in Berk-ss and Hbbth3/+ compared each other and their common background mice (C57BL6/J). Further, spleen and liver macrophages revealed distinct disease specific phenotypes, suggesting macrophages become differentially polarized and reprogrammed within tissue compartments. We conclude that tissue recruitment, polarization, metabolic and proteomic reprogramming of macrophages in Berk-ss and Hbbth3/+ mice may be relevant to disease to progression in other tissue.","INSTITUTE":"University of Colorado School of Medicine","LABORATORY":"Laboratory of Angelo D'Alessandro in collaboratation with David Irwin","LAST_NAME":"Cendali","FIRST_NAME":"Francesca","ADDRESS":"13199 East Montview Boulevard, Aurora, CO, 80045, USA","EMAIL":"francesca.cendali@cuanschutz.edu","PHONE":"3037246131"},

"STUDY":{"STUDY_TITLE":"Metabolic and Proteomic Divergence is Present in Circulating Monocytes and Tissue Resident Macrophages from Berkeley Sickle Cell Anemia and B-thalassemia mice (PBMC's)","STUDY_SUMMARY":"Sickle cell disease and Beta-thalassemia represent hemoglobinopathies arising from dysfunctional or under produced beta-globin chains, respectively. In both diseases, red blood cell injury and anemia are the impetus for end organ injury. Because persistent erythrophagocytosis is a hallmark of these genetic maladies it is critical to understand how macrophage phenotype polarizations in tissue compartments can inform on disease progression. Murine models of sickle cell disease and Beta-thalassemia allow for a basic understanding of mechanisms and provide for translation to human disease. A multi-omics approach to understanding macrophage metabolism and protein changes in two murine models of beta-globinopathy was performed on peripheral blood mononuclear cells as well as spleen and liver macrophages isolated from Berkley sickle cell disease (Berk-ss) and heterozygous B1/B2 globin gene deletion (Hbbth3/+) mice. Results from these experiments revealed the metabolome and proteome of macrophages are polarized to a distinct phenotype in Berk-ss and Hbbth3/+ compared each other and their common background mice (C57BL6/J). Further, spleen and liver macrophages revealed distinct disease specific phenotypes, suggesting macrophages become differentially polarized and reprogrammed within tissue compartments. We conclude that tissue recruitment, polarization, metabolic and proteomic reprogramming of macrophages in Berk-ss and Hbbth3/+ mice may be relevant to disease to progression in other tissue.","INSTITUTE":"University of Colorado School of Medicine","LABORATORY":"Laboratory of Angelo D'Alessandro in collaboratation with David Irwin","LAST_NAME":"Cendali","FIRST_NAME":"Francesca","ADDRESS":"13199 East Montview Boulevard, Aurora, CO, 80045, USA","EMAIL":"francesca.cendali@cuanschutz.edu","PHONE":"3037246131"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"1",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-01"}
},
{
"Subject ID":"-",
"Sample ID":"2",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-02"}
},
{
"Subject ID":"-",
"Sample ID":"3",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-03"}
},
{
"Subject ID":"-",
"Sample ID":"4",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-04"}
},
{
"Subject ID":"-",
"Sample ID":"5",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-05"}
},
{
"Subject ID":"-",
"Sample ID":"6",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-06"}
},
{
"Subject ID":"-",
"Sample ID":"7",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-07"}
},
{
"Subject ID":"-",
"Sample ID":"8",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-08"}
},
{
"Subject ID":"-",
"Sample ID":"9",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-09"}
},
{
"Subject ID":"-",
"Sample ID":"10",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-10"}
},
{
"Subject ID":"-",
"Sample ID":"11",
"Factors":{"Factor":"WT"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-11"}
},
{
"Subject ID":"-",
"Sample ID":"12",
"Factors":{"Factor":"Berk"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-12"}
},
{
"Subject ID":"-",
"Sample ID":"13",
"Factors":{"Factor":"Berk"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-13"}
},
{
"Subject ID":"-",
"Sample ID":"14",
"Factors":{"Factor":"Berk"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-14"}
},
{
"Subject ID":"-",
"Sample ID":"15",
"Factors":{"Factor":"Berk"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-15"}
},
{
"Subject ID":"-",
"Sample ID":"16",
"Factors":{"Factor":"Berk"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-16"}
},
{
"Subject ID":"-",
"Sample ID":"17",
"Factors":{"Factor":"Berk"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-17"}
},
{
"Subject ID":"-",
"Sample ID":"18",
"Factors":{"Factor":"Berk"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-18"}
},
{
"Subject ID":"-",
"Sample ID":"19",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-19"}
},
{
"Subject ID":"-",
"Sample ID":"20",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-20"}
},
{
"Subject ID":"-",
"Sample ID":"21",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-21"}
},
{
"Subject ID":"-",
"Sample ID":"22",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-22"}
},
{
"Subject ID":"-",
"Sample ID":"23",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-23"}
},
{
"Subject ID":"-",
"Sample ID":"24",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-24"}
},
{
"Subject ID":"-",
"Sample ID":"25",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-25"}
},
{
"Subject ID":"-",
"Sample ID":"26",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-26"}
},
{
"Subject ID":"-",
"Sample ID":"27",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-27"}
},
{
"Subject ID":"-",
"Sample ID":"28",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-28"}
},
{
"Subject ID":"-",
"Sample ID":"29",
"Factors":{"Factor":"Beta Thal"},
"Additional sample data":{"RAW_FILE_NAME":"F2-94-29"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Whole blood samples (1 mL) were obtained from animals via cardiac puncture, using a syringe with a 26-gauge needle, and placed in an EDTA treated tube. The blood was transferred to a 15 mL conical tube and diluted 2:1, sterile PBS: blood, and gently mixed. Lympholyte® Mammal Cell Separation media (Cedarlane Labs, product # CL5115) was gently added to the bottom of the blood solution and spun at 1400 rpm for 30 minutes in a refrigerated centrifuge. After centrifugation, layers were visualized, the PBMC layer (midlayer) was extracted and resuspended in a new tube. The isolated PBMC’s were washed using ~14mL sterile PBS, spun at 1800 rpm for 10 minutes, excess PBS was removed, cells were resuspended in 1-2mL for counting, and the final pellet was frozen in liquid nitrogen and stored at -80C.","SAMPLE_TYPE":"Mononuclear cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"Eight- to ten-week-old female C57Bl/6 WT, Berk-ss, or Hbbth3/+ mice were either obtained from Jackson Laboratories (Bar Harbor, ME, USA) or our in-house Berk SCD mouse colony. Mice were housed and bred in an AAALAC accredited animal facility at the University of Colorado, Denver, Anschutz Medical campus and were maintained on a 12:12 light-dark cycle with food and water available ad libitum. Female heterozygous Berk-ss mice were bred with male homozygous Berkss mice to generate homozygous offspring. Specifically, Berk-ss mice with genotype Tg(HuminiLCR α1 Gγ Aγ δ βs ) Hba0/0 Hbb0/0 and the hemizygous with genotype Tg(Hu-miniLCR α1 Gγ Aγ δ βs ) Hba0/0 Hbb0 Hbb+ were littermates. Genotyping of mice used for breeding and experiments was performed by TransnetYX (Cordova, TN, USA). A total of 21 mice (C57Bl/6: n=10, Berk-ss mice: n=11, Hbbth3/+ =10) were used in the present investigation and levels of discomfort and distress were monitored daily by the in-house animal care staff, with a veterinarian available as needed. All experimental procedures were conducted under the guidelines recommended by The Journal of Physiology (11), the National Institutes of Health and were approved by the Institutional Animal Care and Use Committee at the University of Colorado, Denver, Anschutz Medical Campus."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Spleen and liver macrophages and peripheral blood mononuclear cells (PBMCs) were extracted in methanol:acetonitrile:water (5:3:2 v/v/v – at a 1x10^6 cells/ml) ). The samples were vortexed for 30mins at 4°C. The samples were then spun down at 18,213 g for 10 minutes, 4°C and 50uL of supernatant was transferred to autosampler vial."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Positive Ion Mode","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Phenomenex Kinetex C18 (150 x 2.1mm,1.7um)","SOLVENT_A":"100% water; 0.1% formic acid","SOLVENT_B":"100% acetonitrile; 0.1% formic acid","FLOW_GRADIENT":"0-0.5 min 5% B, 0.5-1.1 min 5-95% B, 1.1-2.75 min hold at 95% B, 2.75-3 min 95-5% B, 3-5 min hold at 5% B.","FLOW_RATE":".450ml/min","COLUMN_TEMPERATURE":"45"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Resolution 70,000, scan range 65-900 m/z, maximum injection time 200 ms, microscans 2, automatic gain control (AGC) 3 x 10^6 ions, source voltage 4.0 kV, capillary temperature 320 C, and sheath gas 45, auxiliary gas 15, and sweep gas 0 (all nitrogen). Data converted to mzXML using RawConverter. Metabolites were annotated and integrated using Maven in conjunction with the KEGG database."},

"MS_METABOLITE_DATA":{
"Units":":Peak area",

"Data":[{"Metabolite":"L-alanine","1":"2.83E+06","2":"1.11E+06","3":"4.12E+06","4":"1.01E+06","5":"1.18E+06","6":"1.94E+06","7":"3.50E+06","8":"8.44E+05","9":"6.25E+05","10":"4.26E+06","11":"2.78E+06","12":"3.44E+06","13":"9.18E+05","14":"3.34E+06","15":"2.17E+06","16":"8.85E+06","17":"1.76E+06","18":"8.47E+05","19":"2.23E+06","20":"2.27E+06","21":"4.01E+06","22":"4.97E+06","23":"6.87E+06","24":"1.25E+06","25":"3.29E+06","26":"3.40E+06","27":"4.12E+06","28":"3.26E+06","29":"1.86E+06"},{"Metabolite":"L-arginine","1":"1.08E+05","2":"7.53E+04","3":"5.75E+05","4":"9.77E+04","5":"2.97E+05","6":"7.98E+04","7":"1.31E+05","8":"1.54E+05","9":"1.19E+04","10":"1.72E+05","11":"1.58E+05","12":"8.98E+04","13":"1.22E+05","14":"2.36E+05","15":"4.15E+05","16":"4.04E+05","17":"1.07E+06","18":"2.82E+05","19":"3.56E+05","20":"7.29E+05","21":"1.36E+05","22":"3.28E+05","23":"7.51E+04","24":"2.45E+05","25":"4.99E+05","26":"4.53E+05","27":"4.81E+05","28":"6.59E+05","29":"5.09E+05"},{"Metabolite":"L-glutamate","1":"2.37E+05","2":"1.52E+05","3":"1.86E+06","4":"2.60E+05","5":"7.63E+05","6":"3.54E+05","7":"2.96E+05","8":"8.72E+04","9":"5.32E+04","10":"1.92E+05","11":"4.67E+05","12":"1.05E+05","13":"4.21E+05","14":"5.84E+05","15":"1.05E+06","16":"4.28E+05","17":"9.61E+05","18":"3.18E+05","19":"1.11E+06","20":"1.07E+06","21":"1.90E+05","22":"9.68E+05","23":"3.21E+05","24":"7.04E+05","25":"7.20E+05","26":"1.73E+06","27":"1.22E+06","28":"3.19E+06","29":"2.02E+06"},{"Metabolite":"L-glutamine","1":"5.70E+04","2":"3.60E+04","3":"5.18E+05","4":"7.08E+04","5":"7.14E+04","6":"8.37E+04","7":"3.32E+04","8":"5.79E+04","9":"2.43E+04","10":"3.96E+04","11":"7.66E+04","12":"0.00E+00","13":"5.65E+04","14":"6.50E+04","15":"1.02E+05","16":"2.76E+05","17":"9.06E+04","18":"4.53E+04","19":"1.42E+05","20":"2.25E+05","21":"1.62E+05","22":"1.30E+05","23":"2.77E+05","24":"7.23E+04","25":"1.07E+05","26":"4.35E+05","27":"2.05E+05","28":"1.07E+06","29":"2.60E+05"},{"Metabolite":"L-histidine","1":"3.77E+04","2":"7.70E+04","3":"3.08E+05","4":"8.59E+04","5":"0.00E+00","6":"1.39E+05","7":"1.84E+05","8":"5.94E+04","9":"3.41E+04","10":"5.18E+04","11":"8.01E+04","12":"6.00E+04","13":"7.90E+04","14":"1.26E+05","15":"1.62E+05","16":"8.29E+05","17":"2.53E+05","18":"3.52E+05","19":"1.86E+05","20":"1.48E+05","21":"3.93E+05","22":"2.19E+05","23":"7.71E+05","24":"5.58E+04","25":"1.80E+05","26":"1.62E+05","27":"1.67E+05","28":"2.15E+05","29":"1.56E+05"},{"Metabolite":"L-leucine","1":"4.80E+05","2":"6.54E+05","3":"2.56E+06","4":"4.08E+05","5":"9.47E+05","6":"1.19E+06","7":"5.32E+05","8":"1.78E+06","9":"4.20E+05","10":"1.83E+06","11":"1.53E+06","12":"5.55E+05","13":"6.00E+05","14":"6.29E+05","15":"1.20E+06","16":"2.43E+06","17":"2.58E+06","18":"1.98E+06","19":"1.37E+06","20":"2.73E+06","21":"1.33E+06","22":"1.53E+06","23":"2.30E+06","24":"7.87E+05","25":"1.63E+06","26":"1.35E+06","27":"1.41E+06","28":"1.98E+06","29":"1.77E+06"},{"Metabolite":"L-lysine","1":"9.87E+04","2":"4.13E+04","3":"3.82E+05","4":"8.06E+04","5":"1.27E+05","6":"4.70E+04","7":"4.47E+04","8":"9.03E+04","9":"6.47E+04","10":"1.71E+05","11":"7.24E+04","12":"3.76E+04","13":"9.95E+04","14":"1.23E+05","15":"1.80E+05","16":"3.07E+05","17":"3.94E+05","18":"2.21E+05","19":"2.03E+05","20":"2.47E+05","21":"1.37E+05","22":"1.60E+05","23":"2.43E+05","24":"9.76E+04","25":"1.95E+05","26":"1.74E+05","27":"2.01E+05","28":"2.93E+05","29":"2.18E+05"},{"Metabolite":"L-phenylalanine","1":"1.56E+05","2":"2.72E+05","3":"6.38E+05","4":"2.08E+05","5":"2.47E+05","6":"4.30E+05","7":"2.58E+05","8":"5.80E+05","9":"1.60E+05","10":"7.33E+05","11":"6.62E+05","12":"1.85E+05","13":"1.55E+05","14":"2.05E+05","15":"2.97E+05","16":"1.01E+06","17":"1.09E+06","18":"1.00E+06","19":"3.65E+05","20":"5.46E+05","21":"4.55E+05","22":"7.14E+05","23":"7.75E+05","24":"3.17E+05","25":"3.68E+05","26":"3.76E+05","27":"4.46E+05","28":"5.13E+05","29":"4.30E+05"},{"Metabolite":"L-proline","1":"6.05E+05","2":"4.21E+05","3":"3.09E+06","4":"6.23E+05","5":"1.05E+06","6":"5.29E+05","7":"7.54E+05","8":"6.68E+05","9":"3.07E+05","10":"4.26E+05","11":"7.04E+05","12":"5.09E+05","13":"8.42E+05","14":"1.14E+06","15":"2.07E+06","16":"6.70E+06","17":"2.40E+06","18":"1.32E+06","19":"1.78E+06","20":"2.23E+06","21":"2.54E+06","22":"2.15E+06","23":"4.79E+06","24":"7.76E+05","25":"1.76E+06","26":"2.24E+06","27":"2.23E+06","28":"2.53E+06","29":"2.57E+06"},{"Metabolite":"L-serine","1":"5.93E+04","2":"3.82E+04","3":"1.26E+05","4":"5.85E+04","5":"9.19E+04","6":"3.76E+04","7":"4.69E+04","8":"5.78E+04","9":"4.33E+04","10":"4.73E+04","11":"7.33E+04","12":"6.09E+04","13":"7.50E+04","14":"9.28E+04","15":"1.69E+05","16":"1.82E+05","17":"1.31E+05","18":"2.40E+05","19":"2.43E+05","20":"1.80E+05","21":"8.94E+04","22":"1.92E+05","23":"1.48E+05","24":"8.41E+04","25":"1.51E+05","26":"2.08E+05","27":"2.23E+05","28":"3.68E+05","29":"1.88E+05"},{"Metabolite":"L-threonine","1":"8.64E+04","2":"8.77E+04","3":"5.37E+05","4":"1.39E+05","5":"2.52E+05","6":"8.16E+04","7":"1.15E+05","8":"9.75E+04","9":"6.38E+04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ID":"C02862","M/Z":"232.1542","r.t.":"0.36"},{"Metabolite":"acyl-C4-OH (Hydroxybutyrylcarnitine)","Kegg ID":"HMDB13127","M/Z":"248.1488","r.t.":"0.20"},{"Metabolite":"hexanoyl-L-carnitine (acyl-C6)","Kegg ID":"HMDB00756","M/Z":"260.1855","r.t.":"0.63"},{"Metabolite":"O-dodecanoyl-carnitine (acyl-C12)","Kegg ID":"HMDB02250","M/Z":"344.2788","r.t.":"0.75"},{"Metabolite":"O-tetradecanoyl-L-carnitine (acyl-C14)","Kegg ID":"HMDB05066","M/Z":"372.3102","r.t.":"0.78"},{"Metabolite":"Tetradecenoyl Carnitine (acyl-C14:1)","Kegg ID":"HMDB13329","M/Z":"370.2946","r.t.":"0.76"},{"Metabolite":"L-Palmitoylcarnitine (acyl-C16)","Kegg ID":"C02990","M/Z":"400.3411","r.t.":"0.83"},{"Metabolite":"Hexadecenoyl-carnitine (acyl-C16:1)","Kegg ID":"HMDB06317","M/Z":"398.3258","r.t.":"0.80"},{"Metabolite":"O-octadecenoyl-L-carnitine (acyl-C18:1)","Kegg ID":"HMDB06351","M/Z":"426.3567","r.t.":"0.84"},{"Metabolite":"acyl-C18:2 (Linoleoyl-CoA)","Kegg ID":"HMDB01064","M/Z":"424.3410","r.t.":"0.81"},{"Metabolite":"acyl-C18:2-OH","Kegg ID":"ac123","M/Z":"440.3368","r.t.":"0.75"},{"Metabolite":"acyl-C20:4","Kegg ID":"ac125","M/Z":"448.3410","r.t.":"0.81"},{"Metabolite":"Acetylcarnitine","Kegg ID":"HMDB0000201","M/Z":"204.1229","r.t.":"0.172105"},{"Metabolite":"Isovalerylcarnitine","Kegg ID":"HMDB0000688","M/Z":"246.1698","r.t.":"0.6123381"},{"Metabolite":"Hexenoylcarnitine","Kegg ID":"HMDB0013161","M/Z":"258.1697","r.t.":"0.6563342"},{"Metabolite":"Hexanoylcarnitine","Kegg ID":"HMDB0000705","M/Z":"260.1855","r.t.":"0.6275252"},{"Metabolite":"Dimethylheptanoyl carnitine","Kegg ID":"HMDB0006320","M/Z":"302.2322","r.t.":"0.8198106"},{"Metabolite":"Pimelylcarnitine","Kegg ID":"HMDB0013328","M/Z":"304.1752","r.t.":"0.6294311"},{"Metabolite":"Hydroxyoctanoyl carnitine","Kegg ID":"HMDB0061634","M/Z":"304.211","r.t.":"0.6134121"},{"Metabolite":"Decanoylcarnitine","Kegg ID":"HMDB0000651","M/Z":"316.2478","r.t.":"0.7397916"},{"Metabolite":"Hydroxynonanoyl carnitine","Kegg ID":"HMDB0061635","M/Z":"318.2267","r.t.":"0.6402744"},{"Metabolite":"Dimethylnonanoyl carnitine","Kegg ID":"HMDB0006202","M/Z":"330.2632","r.t.":"0.8124963"},{"Metabolite":"Hydroxydecanoyl carnitine","Kegg ID":"HMDB0061636","M/Z":"332.2427","r.t.":"0.6854022"},{"Metabolite":"Hydroxydodecanoyl carnitine","Kegg ID":"HMDB0061638","M/Z":"360.2733","r.t.":"0.7055764"},{"Metabolite":"Tetradecenoylcarnitine","Kegg ID":"HMDB0062588","M/Z":"370.2946","r.t.":"0.759873"},{"Metabolite":"Tetradecanoylcarnitine","Kegg ID":"HMDB0005066","M/Z":"372.3103","r.t.":"0.7853739"},{"Metabolite":"Hydroxytetradecenoylcarnitine","Kegg ID":"HMDB0013330","M/Z":"386.2892","r.t.":"0.7253172"},{"Metabolite":"Pentadecanoylcarnitine","Kegg ID":"HMDB0062517","M/Z":"386.3256","r.t.":"0.8065909"},{"Metabolite":"Hydroxytetradecanoyl carnitine","Kegg ID":"HMDB0061640","M/Z":"388.3046","r.t.":"0.734615"},{"Metabolite":"Hydroxyhexadecanoyl carnitine","Kegg ID":"HMDB0061642","M/Z":"416.3359","r.t.":"0.7778495"}]
}

}