{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002740","ANALYSIS_ID":"AN004443","VERSION":"1","CREATED_ON":"June 21, 2023, 3:41 pm"},

"PROJECT":{"PROJECT_TITLE":"Acetate acts as a metabolic immunomodulator that potentiates anti-tumour immunity in breast cancer","PROJECT_SUMMARY":"Acetate metabolism is an important metabolic pathway in many cancers and is controlled by acetyl-CoA synthetase 2 (ACSS2), an enzyme that catalyzes the conversion of acetate to acetyl-CoA. While the metabolic role of ACSS2 in cancer is well described, the consequences of blocking tumour acetate metabolism on the tumour microenvironment and anti-tumour immunity are unknown. We demonstrate that blocking ACSS2 switches cancer cells from acetate consumers to producers of acetate thereby freeing acetate for tumour-infiltrating lymphocytes to use as a fuel source. We show that acetate supplementation metabolically bolsters T-cell effector functions and proliferation. Targeting ACSS2 with CRISPR-Cas9 guides or a small molecule inhibitor promotes an anti-tumour immune response and enhances the efficacy of chemotherapy in preclinical breast cancer models. We propose a novel paradigm for targeting acetate metabolism in cancer in which inhibition of ACSS2 dually acts to impair tumour cell metabolism and potentiate anti-tumour immunity.","INSTITUTE":"The Wistar Institute","LAST_NAME":"Schug","FIRST_NAME":"Zachary","ADDRESS":"3601 Spruce St, Philadelphia PA 19104","EMAIL":"zschug@wistar.org","PHONE":"215-898-3705"},

"STUDY":{"STUDY_TITLE":"Non-targeted metabolomics screen comparing 13C2-acetate labeling of metabolites in CD8+ T cells and NK cells from mouse spleens.","STUDY_SUMMARY":"Non-targeted metabolomics screen comparing 13C2-acetate labeling of metabolites in CD8+ T cells and NK cells from mouse spleens (wild type vs ACSS2 knockout C57Bl/6 mice). Metabolites were analyzed using a high-resolution, high-performance LC-MS analysis.","INSTITUTE":"The Wistar Institute","LAST_NAME":"Schug","FIRST_NAME":"Zachary","ADDRESS":"3601 Spruce St, Philadelphia PA 19104","EMAIL":"zschug@wistar.org","PHONE":"215-898-3705"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57Bl/6"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Sample_1-100_1",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_1-100_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_1-100_2",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_1-100_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_1-100_3",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_1-100_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_1-500_1",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_1-500_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_1-500_2",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_1-500_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_1-500_3",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_1-500_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_2-100_1",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_2-100_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_2-100_2",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_2-100_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_2-100_3",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_2-100_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_2-500_1",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_2-500_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_2-500_2",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_2-500_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_2-500_3",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_2-500_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO1-100_1",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO1-100_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO1-100_2",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO1-100_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO1-100_3",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO1-100_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO1-500_1",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO1-500_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO1-500_2",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO1-500_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO1-500_3",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO1-500_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO2-100_1",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO2-100_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO2-100_2",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO2-100_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO2-100_3",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO2-100_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO2-500_1",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO2-500_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO2-500_2",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO2-500_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO2-500_3",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO2-500_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO2-500_IN_1",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"Y"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO2-500_IN_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO2-500_IN_2",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"Y"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO2-500_IN_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_KO2-500_IN_3",
"Factors":{"Mouse model":"Acss2-/- C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"Y"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_KO2-500_IN_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_N3-100_1",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"NK cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_N3-100_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_N3-100_2",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"NK cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_N3-100_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_N3-100_3",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"NK cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_N3-100_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_N3-500_1",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"NK cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_N3-500_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_N3-500_2",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"NK cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_N3-500_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_N3-500_3",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"NK cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_N3-500_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_WT2-100_1",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_WT2-100_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_WT2-100_2",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_WT2-100_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_WT2-100_3",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"100","Time (hours)":"1","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_WT2-100_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_WT2-500_1",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_WT2-500_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_WT2-500_2",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_WT2-500_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_WT2-500_3",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"N"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_WT2-500_3.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_WT2-500-IN_1",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"Y"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_WT2-500-IN_1.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_WT2-500-IN_2",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"Y"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_WT2-500-IN_2.raw"}
},
{
"Subject ID":"-",
"Sample ID":"Sample_WT2-500-IN_3",
"Factors":{"Mouse model":"WT C57Bl/6","Cell Type":"CD8+ T cells","[13C2-acetate] in mM":"500","Time (hours)":"2","ACSS2 inhibitor (Y/N)":"Y"},
"Additional sample data":{"RAW_FILE_NAME":"Schug_m22-L258_Sample_WT2-500-IN_3.raw"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"CD8+ T cells and NK cells were purified by negative selection from freshly prepared mouse splenocytes (wild type vs ACSS2 knockout C57Bl/6 mice).","SAMPLE_TYPE":"Splenocytes"},

"TREATMENT":{"TREATMENT_SUMMARY":"Cells were incubated up to 3 hours in the in 100 or 500 µM 13C2-acetate and treated with vehicle (DMSO) or an ACSS2 inhibitor (VY-3-135)."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Cells were pelleted and washed with ice-cold PBS before extraction of metabolites in 80% methanol. Samples were centrifuged at 4 °C two times (18,000 x g). Deproteinated supernatants were stored at -80 °C prior to analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Hydrophilic interaction liquid chromatography (HILIC) was performed at 0.2 ml/min on a ZIC-pHILIC column (150 x 2.1 mm, 5 µM particle size, EMD Millipore) with a ZIC-pHILIC guard column (20 x 2.1 mm, EMD Millipore) at 30 °C. Solvent A was 20 mM ammonium carbonate, 0.1% ammonium hydroxide, pH 9.2, 5 µM medronic acid, and solvent B was acetonitrile. The gradient was 85% B for 2 min, 85% B to 20% B over 15 min, 20% B to 85% B over 0.1 min, and 85% B for 8.9 min. The autosampler was held at 4 °C. For each analysis, 5 µl of sample was injected.","CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"SeQuant ZIC-pHILIC (150 x 2.1mm, 5um)","SOLVENT_A":"20 mM ammonium carbonate, 0.1% ammonium hydroxide, pH 9.2, 5 µM medronic acid","SOLVENT_B":"acetonitrile","FLOW_GRADIENT":"85% B for 2 min, 85% B to 20% B over 15 min, 20% B to 85% B over 0.1 min, and 85% B for 8.9 min","FLOW_RATE":"0.2 ml/min","COLUMN_TEMPERATURE":"30"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF-X Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"The following parameters were used for the MS analysis: sheath gas flow rate, 30; auxiliary gas flow rate, 5; sweep gas flow rate, 0; auxiliary gas heater temperature, 200 °C; spray voltage, 3.6 kV for positive and negative modes; capillary temperature, 325 °C; and funnel RF level, 65. Samples were analyzed by full MS with polarity switching. Full MS scans were acquired at 60,000 resolution with 2 microscans and a scan range of 72-1080 m/z, automatic gain control (AGC) target of 5e6, and maximum injection time (IT) of 200 ms."},

"MS_METABOLITE_DATA":{
"Units":"Peak Area",

"Data":[{"Metabolite":"alpha-Ketoglutaric acid","Sample_1-100_1":"3543113","Sample_1-100_2":"3463554","Sample_1-100_3":"3380395","Sample_1-500_1":"1635549","Sample_1-500_2":"1336395","Sample_1-500_3":"1447294","Sample_2-100_1":"10603245","Sample_2-100_2":"10148443","Sample_2-100_3":"11102770","Sample_2-500_1":"7394011","Sample_2-500_2":"6965021","Sample_2-500_3":"7738371","Sample_KO1-100_1":"5046568","Sample_KO1-100_2":"4461495","Sample_KO1-100_3":"4475825","Sample_KO1-500_1":"4952437","Sample_KO1-500_2":"4751793","Sample_KO1-500_3":"5342288","Sample_KO2-100_1":"3273977","Sample_KO2-100_2":"3679792","Sample_KO2-100_3":"3814855","Sample_KO2-500_1":"2154854","Sample_KO2-500_2":"2186893","Sample_KO2-500_3":"2219570","Sample_KO2-500_IN_1":"2022788","Sample_KO2-500_IN_2":"1792595","Sample_KO2-500_IN_3":"1857396","Sample_N3-100_1":"7593082","Sample_N3-100_2":"7651623","Sample_N3-100_3":"7561177","Sample_N3-500_1":"4976112","Sample_N3-500_2":"5623879","Sample_N3-500_3":"5525904","Sample_WT2-100_1":"5170933","Sample_WT2-100_2":"4901837","Sample_WT2-100_3":"4719258","Sample_WT2-500_1":"2571844","Sample_WT2-500_2":"2217707","Sample_WT2-500_3":"2537994","Sample_WT2-500-IN_1":"3156367","Sample_WT2-500-IN_2":"3660426","Sample_WT2-500-IN_3":"3420260"},{"Metabolite":"alpha-Ketoglutaric acid +1","Sample_1-100_1":"388356","Sample_1-100_2":"416414","Sample_1-100_3":"431622","Sample_1-500_1":"198354","Sample_1-500_2":"150990","Sample_1-500_3":"191159","Sample_2-100_1":"1549945","Sample_2-100_2":"1466776","Sample_2-100_3":"1624480","Sample_2-500_1":"966050","Sample_2-500_2":"968229","Sample_2-500_3":"1062093","Sample_KO1-100_1":"389147","Sample_KO1-100_2":"356487","Sample_KO1-100_3":"343955","Sample_KO1-500_1":"665975","Sample_KO1-500_2":"652499","Sample_KO1-500_3":"721825","Sample_KO2-100_1":"418069","Sample_KO2-100_2":"485641","Sample_KO2-100_3":"509233","Sample_KO2-500_1":"388290","Sample_KO2-500_2":"336737","Sample_KO2-500_3":"397988","Sample_KO2-500_IN_1":"359596","Sample_KO2-500_IN_2":"290407","Sample_KO2-500_IN_3":"371441","Sample_N3-100_1":"914882","Sample_N3-100_2":"894871","Sample_N3-100_3":"859933","Sample_N3-500_1":"862912","Sample_N3-500_2":"1012521","Sample_N3-500_3":"974252","Sample_WT2-100_1":"738516","Sample_WT2-100_2":"739019","Sample_WT2-100_3":"684569","Sample_WT2-500_1":"379025","Sample_WT2-500_2":"349838","Sample_WT2-500_3":"392478","Sample_WT2-500-IN_1":"579168","Sample_WT2-500-IN_2":"672009","Sample_WT2-500-IN_3":"635747"},{"Metabolite":"alpha-Ketoglutaric acid +2","Sample_1-100_1":"888435","Sample_1-100_2":"855326","Sample_1-100_3":"918602","Sample_1-500_1":"1266328","Sample_1-500_2":"1043645","Sample_1-500_3":"1159956","Sample_2-100_1":"2301375","Sample_2-100_2":"2154035","Sample_2-100_3":"2381049","Sample_2-500_1":"4365700","Sample_2-500_2":"4287511","Sample_2-500_3":"4546739","Sample_KO1-100_1":"861749","Sample_KO1-100_2":"823710","Sample_KO1-100_3":"795607","Sample_KO1-500_1":"3520798","Sample_KO1-500_2":"3416320","Sample_KO1-500_3":"3754869","Sample_KO2-100_1":"887874","Sample_KO2-100_2":"1057000","Sample_KO2-100_3":"1040599","Sample_KO2-500_1":"1660643","Sample_KO2-500_2":"1608780","Sample_KO2-500_3":"1676110","Sample_KO2-500_IN_1":"1460451","Sample_KO2-500_IN_2":"1246630","Sample_KO2-500_IN_3":"1398342","Sample_N3-100_1":"1146534","Sample_N3-100_2":"1110882","Sample_N3-100_3":"1027539","Sample_N3-500_1":"1933393","Sample_N3-500_2":"2188014","Sample_N3-500_3":"2042067","Sample_WT2-100_1":"1392512","Sample_WT2-100_2":"1333337","Sample_WT2-100_3":"1189251","Sample_WT2-500_1":"2042517","Sample_WT2-500_2":"1885047","Sample_WT2-500_3":"1843113","Sample_WT2-500-IN_1":"2496338","Sample_WT2-500-IN_2":"2771282","Sample_WT2-500-IN_3":"2524961"},{"Metabolite":"alpha-Ketoglutaric acid +3","Sample_1-100_1":"150725","Sample_1-100_2":"115735","Sample_1-100_3":"157431","Sample_1-500_1":"542495","Sample_1-500_2":"434535","Sample_1-500_3":"473057","Sample_2-100_1":"510221","Sample_2-100_2":"460027","Sample_2-100_3":"506847","Sample_2-500_1":"2363170","Sample_2-500_2":"2247425","Sample_2-500_3":"2449246","Sample_KO1-100_1":"93427","Sample_KO1-100_2":"70737","Sample_KO1-100_3":"87406","Sample_KO1-500_1":"1145812","Sample_KO1-500_2":"1045604","Sample_KO1-500_3":"1181428","Sample_KO2-100_1":"161372","Sample_KO2-100_2":"193986","Sample_KO2-100_3":"199924","Sample_KO2-500_1":"913184","Sample_KO2-500_2":"794977","Sample_KO2-500_3":"893305","Sample_KO2-500_IN_1":"817508","Sample_KO2-500_IN_2":"689919","Sample_KO2-500_IN_3":"849471","Sample_N3-100_1":"167033","Sample_N3-100_2":"148431","Sample_N3-100_3":"149868","Sample_N3-500_1":"980764","Sample_N3-500_2":"1182938","Sample_N3-500_3":"1105672","Sample_WT2-100_1":"282092","Sample_WT2-100_2":"228197","Sample_WT2-100_3":"258634","Sample_WT2-500_1":"1169018","Sample_WT2-500_2":"1018573","Sample_WT2-500_3":"1080114","Sample_WT2-500-IN_1":"1313047","Sample_WT2-500-IN_2":"1489090","Sample_WT2-500-IN_3":"1427643"},{"Metabolite":"alpha-Ketoglutaric acid +4","Sample_1-100_1":"73331","Sample_1-100_2":"90088","Sample_1-100_3":"89277","Sample_1-500_1":"697583","Sample_1-500_2":"538814","Sample_1-500_3":"629965","Sample_2-100_1":"198111","Sample_2-100_2":"207168","Sample_2-100_3":"284301","Sample_2-500_1":"2904274","Sample_2-500_2":"3044734","Sample_2-500_3":"3333867","Sample_KO1-100_1":"51448","Sample_KO1-100_2":"0","Sample_KO1-100_3":"19797","Sample_KO1-500_1":"1215909","Sample_KO1-500_2":"1166229","Sample_KO1-500_3":"1390234","Sample_KO2-100_1":"74545","Sample_KO2-100_2":"121623","Sample_KO2-100_3":"137501","Sample_KO2-500_1":"1088496","Sample_KO2-500_2":"1101060","Sample_KO2-500_3":"1151782","Sample_KO2-500_IN_1":"979553","Sample_KO2-500_IN_2":"896176","Sample_KO2-500_IN_3":"887842","Sample_N3-100_1":"0","Sample_N3-100_2":"0","Sample_N3-100_3":"45423","Sample_N3-500_1":"946032","Sample_N3-500_2":"1021134","Sample_N3-500_3":"1022704","Sample_WT2-100_1":"121027","Sample_WT2-100_2":"107473","Sample_WT2-100_3":"104828","Sample_WT2-500_1":"1802942","Sample_WT2-500_2":"1412769","Sample_WT2-500_3":"1562678","Sample_WT2-500-IN_1":"1605076","Sample_WT2-500-IN_2":"2076981","Sample_WT2-500-IN_3":"1881161"},{"Metabolite":"alpha-Ketoglutaric acid +5","Sample_1-100_1":"0","Sample_1-100_2":"0","Sample_1-100_3":"0","Sample_1-500_1":"236811","Sample_1-500_2":"185488","Sample_1-500_3":"233910","Sample_2-100_1":"0","Sample_2-100_2":"24650","Sample_2-100_3":"57060","Sample_2-500_1":"1612579","Sample_2-500_2":"1491070","Sample_2-500_3":"1655055","Sample_KO1-100_1":"0","Sample_KO1-100_2":"32633","Sample_KO1-100_3":"0","Sample_KO1-500_1":"266494","Sample_KO1-500_2":"195687","Sample_KO1-500_3":"317211","Sample_KO2-100_1":"24993","Sample_KO2-100_2":"0","Sample_KO2-100_3":"40026","Sample_KO2-500_1":"506557","Sample_KO2-500_2":"463706","Sample_KO2-500_3":"483198","Sample_KO2-500_IN_1":"479209","Sample_KO2-500_IN_2":"422345","Sample_KO2-500_IN_3":"494910","Sample_N3-100_1":"36451","Sample_N3-100_2":"37585","Sample_N3-100_3":"30512","Sample_N3-500_1":"492979","Sample_N3-500_2":"527775","Sample_N3-500_3":"526241","Sample_WT2-100_1":"0","Sample_WT2-100_2":"36683","Sample_WT2-100_3":"26984","Sample_WT2-500_1":"986267","Sample_WT2-500_2":"802793","Sample_WT2-500_3":"996798","Sample_WT2-500-IN_1":"733429","Sample_WT2-500-IN_2":"913515","Sample_WT2-500-IN_3":"834444"},{"Metabolite":"Citric acid","Sample_1-100_1":"127072208","Sample_1-100_2":"112042739","Sample_1-100_3":"128108646","Sample_1-500_1":"83090462","Sample_1-500_2":"71009077","Sample_1-500_3":"90796731","Sample_2-100_1":"155079417","Sample_2-100_2":"157638603","Sample_2-100_3":"173870940","Sample_2-500_1":"88984217","Sample_2-500_2":"109532620","Sample_2-500_3":"121170780","Sample_KO1-100_1":"176934435","Sample_KO1-100_2":"171998837","Sample_KO1-100_3":"166423811","Sample_KO1-500_1":"117225998","Sample_KO1-500_2":"112819647","Sample_KO1-500_3":"128164907","Sample_KO2-100_1":"157390311","Sample_KO2-100_2":"178658640","Sample_KO2-100_3":"180730302","Sample_KO2-500_1":"112036398","Sample_KO2-500_2":"127319587","Sample_KO2-500_3":"121559584","Sample_KO2-500_IN_1":"106893039","Sample_KO2-500_IN_2":"110359765","Sample_KO2-500_IN_3":"113877662","Sample_N3-100_1":"196014435","Sample_N3-100_2":"242383169","Sample_N3-100_3":"248219185","Sample_N3-500_1":"137999260","Sample_N3-500_2":"137434213","Sample_N3-500_3":"149385872","Sample_WT2-100_1":"183723076","Sample_WT2-100_2":"193299273","Sample_WT2-100_3":"202858826","Sample_WT2-500_1":"113178608","Sample_WT2-500_2":"106626398","Sample_WT2-500_3":"123850030","Sample_WT2-500-IN_1":"118884168","Sample_WT2-500-IN_2":"126387735","Sample_WT2-500-IN_3":"133705190"},{"Metabolite":"Citric acid +1","Sample_1-100_1":"15424230","Sample_1-100_2":"14685391","Sample_1-100_3":"16582454","Sample_1-500_1":"9628921","Sample_1-500_2":"8763468","Sample_1-500_3":"9751632","Sample_2-100_1":"26810656","Sample_2-100_2":"28255609","Sample_2-100_3":"29573867","Sample_2-500_1":"15740179","Sample_2-500_2":"17827555","Sample_2-500_3":"19283728","Sample_KO1-100_1":"18970319","Sample_KO1-100_2":"18797701","Sample_KO1-100_3":"16948654","Sample_KO1-500_1":"17269860","Sample_KO1-500_2":"16155972","Sample_KO1-500_3":"19448023","Sample_KO2-100_1":"25243008","Sample_KO2-100_2":"27708495","Sample_KO2-100_3":"28032258","Sample_KO2-500_1":"26215586","Sample_KO2-500_2":"29637231","Sample_KO2-500_3":"27518417","Sample_KO2-500_IN_1":"24749417","Sample_KO2-500_IN_2":"25537495","Sample_KO2-500_IN_3":"26020566","Sample_N3-100_1":"28914620","Sample_N3-100_2":"34684695","Sample_N3-100_3":"33058657","Sample_N3-500_1":"33738335","Sample_N3-500_2":"33996188","Sample_N3-500_3":"34497047","Sample_WT2-100_1":"29687230","Sample_WT2-100_2":"30215116","Sample_WT2-100_3":"30065735","Sample_WT2-500_1":"26944274","Sample_WT2-500_2":"24507267","Sample_WT2-500_3":"23495556","Sample_WT2-500-IN_1":"28083812","Sample_WT2-500-IN_2":"33013658","Sample_WT2-500-IN_3":"31325744"},{"Metabolite":"Citric acid +2","Sample_1-100_1":"28939502","Sample_1-100_2":"26455043","Sample_1-100_3":"29660553","Sample_1-500_1":"47203259","Sample_1-500_2":"42122697","Sample_1-500_3":"44255311","Sample_2-100_1":"50219529","Sample_2-100_2":"48572643","Sample_2-100_3":"55716364","Sample_2-500_1":"80034103","Sample_2-500_2":"92151000","Sample_2-500_3":"98542303","Sample_KO1-100_1":"43993467","Sample_KO1-100_2":"42844920","Sample_KO1-100_3":"38809262","Sample_KO1-500_1":"106354375","Sample_KO1-500_2":"102952794","Sample_KO1-500_3":"109571409","Sample_KO2-100_1":"36679198","Sample_KO2-100_2":"40612562","Sample_KO2-100_3":"40700215","Sample_KO2-500_1":"81214788","Sample_KO2-500_2":"84777306","Sample_KO2-500_3":"80673695","Sample_KO2-500_IN_1":"68423561","Sample_KO2-500_IN_2":"72279518","Sample_KO2-500_IN_3":"72791703","Sample_N3-100_1":"43499844","Sample_N3-100_2":"49006441","Sample_N3-100_3":"48243644","Sample_N3-500_1":"83716783","Sample_N3-500_2":"83401966","Sample_N3-500_3":"84099508","Sample_WT2-100_1":"44583786","Sample_WT2-100_2":"41077627","Sample_WT2-100_3":"42513973","Sample_WT2-500_1":"87162158","Sample_WT2-500_2":"80385140","Sample_WT2-500_3":"80706127","Sample_WT2-500-IN_1":"84734695","Sample_WT2-500-IN_2":"103438650","Sample_WT2-500-IN_3":"98064173"},{"Metabolite":"Citric acid +3","Sample_1-100_1":"5399300","Sample_1-100_2":"8668010","Sample_1-100_3":"9307013","Sample_1-500_1":"16633060","Sample_1-500_2":"15513320","Sample_1-500_3":"18465279","Sample_2-100_1":"13790726","Sample_2-100_2":"13481252","Sample_2-100_3":"13585022","Sample_2-500_1":"35495358","Sample_2-500_2":"39735887","Sample_2-500_3":"43799045","Sample_KO1-100_1":"5363846","Sample_KO1-100_2":"5758183","Sample_KO1-100_3":"5239261","Sample_KO1-500_1":"30343876","Sample_KO1-500_2":"29486602","Sample_KO1-500_3":"33239868","Sample_KO2-100_1":"10807900","Sample_KO2-100_2":"11666992","Sample_KO2-100_3":"12750370","Sample_KO2-500_1":"54149802","Sample_KO2-500_2":"57756766","Sample_KO2-500_3":"56794818","Sample_KO2-500_IN_1":"51543050","Sample_KO2-500_IN_2":"51755174","Sample_KO2-500_IN_3":"53031780","Sample_N3-100_1":"7845557","Sample_N3-100_2":"10447664","Sample_N3-100_3":"10008360","Sample_N3-500_1":"48199668","Sample_N3-500_2":"50005484","Sample_N3-500_3":"50653524","Sample_WT2-100_1":"11527614","Sample_WT2-100_2":"12775892","Sample_WT2-100_3":"12044478","Sample_WT2-500_1":"64286344","Sample_WT2-500_2":"60480551","Sample_WT2-500_3":"61622595","Sample_WT2-500-IN_1":"62250432","Sample_WT2-500-IN_2":"69925190","Sample_WT2-500-IN_3":"69776627"},{"Metabolite":"Citric acid +4","Sample_1-100_1":"2529424","Sample_1-100_2":"4743281","Sample_1-100_3":"5097414","Sample_1-500_1":"27075593","Sample_1-500_2":"25332290","Sample_1-500_3":"26979182","Sample_2-100_1":"8191610","Sample_2-100_2":"8505135","Sample_2-100_3":"9336337","Sample_2-500_1":"49378039","Sample_2-500_2":"54107372","Sample_2-500_3":"60391242","Sample_KO1-100_1":"3406445","Sample_KO1-100_2":"3422257","Sample_KO1-100_3":"3309303","Sample_KO1-500_1":"47449091","Sample_KO1-500_2":"45816625","Sample_KO1-500_3":"50917007","Sample_KO2-100_1":"3997610","Sample_KO2-100_2":"4213098","Sample_KO2-100_3":"4631639","Sample_KO2-500_1":"45656110","Sample_KO2-500_2":"48023138","Sample_KO2-500_3":"46128500","Sample_KO2-500_IN_1":"41061778","Sample_KO2-500_IN_2":"41944218","Sample_KO2-500_IN_3":"42083041","Sample_N3-100_1":"3237882","Sample_N3-100_2":"4375695","Sample_N3-100_3":"4330231","Sample_N3-500_1":"40519745","Sample_N3-500_2":"40670404","Sample_N3-500_3":"39658277","Sample_WT2-100_1":"3021811","Sample_WT2-100_2":"3783686","Sample_WT2-100_3":"3734726","Sample_WT2-500_1":"61658145","Sample_WT2-500_2":"58083940","Sample_WT2-500_3":"61704544","Sample_WT2-500-IN_1":"50027607","Sample_WT2-500-IN_2":"57444260","Sample_WT2-500-IN_3":"60307189"},{"Metabolite":"Citric acid +5","Sample_1-100_1":"381920","Sample_1-100_2":"1406309","Sample_1-100_3":"1703780","Sample_1-500_1":"19270992","Sample_1-500_2":"16426950","Sample_1-500_3":"19189202","Sample_2-100_1":"2216710","Sample_2-100_2":"2529341","Sample_2-100_3":"2754402","Sample_2-500_1":"34068873","Sample_2-500_2":"38026969","Sample_2-500_3":"41342721","Sample_KO1-100_1":"355707","Sample_KO1-100_2":"476923","Sample_KO1-100_3":"385773","Sample_KO1-500_1":"24608022","Sample_KO1-500_2":"21768721","Sample_KO1-500_3":"25647766","Sample_KO2-100_1":"822619","Sample_KO2-100_2":"840717","Sample_KO2-100_3":"989059","Sample_KO2-500_1":"24932450","Sample_KO2-500_2":"26004722","Sample_KO2-500_3":"26046357","Sample_KO2-500_IN_1":"22277827","Sample_KO2-500_IN_2":"23314304","Sample_KO2-500_IN_3":"23590567","Sample_N3-100_1":"422770","Sample_N3-100_2":"1082161","Sample_N3-100_3":"931690","Sample_N3-500_1":"21250558","Sample_N3-500_2":"21411383","Sample_N3-500_3":"21003755","Sample_WT2-100_1":"524622","Sample_WT2-100_2":"700778","Sample_WT2-100_3":"858916","Sample_WT2-500_1":"34550608","Sample_WT2-500_2":"31351857","Sample_WT2-500_3":"31884848","Sample_WT2-500-IN_1":"24797212","Sample_WT2-500-IN_2":"30625010","Sample_WT2-500-IN_3":"29379794"},{"Metabolite":"Citric acid +6","Sample_1-100_1":"0","Sample_1-100_2":"181530","Sample_1-100_3":"276927","Sample_1-500_1":"5261371","Sample_1-500_2":"4803200","Sample_1-500_3":"5093050","Sample_2-100_1":"140641","Sample_2-100_2":"178459","Sample_2-100_3":"82508","Sample_2-500_1":"16755031","Sample_2-500_2":"18902454","Sample_2-500_3":"20509395","Sample_KO1-100_1":"0","Sample_KO1-100_2":"0","Sample_KO1-100_3":"0","Sample_KO1-500_1":"3994530","Sample_KO1-500_2":"3604117","Sample_KO1-500_3":"3906865","Sample_KO2-100_1":"0","Sample_KO2-100_2":"0","Sample_KO2-100_3":"0","Sample_KO2-500_1":"9806887","Sample_KO2-500_2":"10539306","Sample_KO2-500_3":"10584977","Sample_KO2-500_IN_1":"8828281","Sample_KO2-500_IN_2":"9241615","Sample_KO2-500_IN_3":"9546732","Sample_N3-100_1":"0","Sample_N3-100_2":"0","Sample_N3-100_3":"0","Sample_N3-500_1":"10280975","Sample_N3-500_2":"10073373","Sample_N3-500_3":"10675735","Sample_WT2-100_1":"0","Sample_WT2-100_2":"0","Sample_WT2-100_3":"0","Sample_WT2-500_1":"18085435","Sample_WT2-500_2":"16341520","Sample_WT2-500_3":"17145539","Sample_WT2-500-IN_1":"10644216","Sample_WT2-500-IN_2":"12658575","Sample_WT2-500-IN_3":"12384441"},{"Metabolite":"Glutamic acid","Sample_1-100_1":"510064773","Sample_1-100_2":"506689201","Sample_1-100_3":"496785551","Sample_1-500_1":"290322016","Sample_1-500_2":"243766993","Sample_1-500_3":"243345781","Sample_2-100_1":"947956479","Sample_2-100_2":"936865362","Sample_2-100_3":"960066216","Sample_2-500_1":"657071066","Sample_2-500_2":"674371834","Sample_2-500_3":"682560362","Sample_KO1-100_1":"804788110","Sample_KO1-100_2":"751346748","Sample_KO1-100_3":"744455453","Sample_KO1-500_1":"547774678","Sample_KO1-500_2":"552925485","Sample_KO1-500_3":"583707259","Sample_KO2-100_1":"716053096","Sample_KO2-100_2":"722404323","Sample_KO2-100_3":"692731825","Sample_KO2-500_1":"507489838","Sample_KO2-500_2":"496564658","Sample_KO2-500_3":"479126365","Sample_KO2-500_IN_1":"490677338","Sample_KO2-500_IN_2":"445387367","Sample_KO2-500_IN_3":"455795926","Sample_N3-100_1":"959948534","Sample_N3-100_2":"910645109","Sample_N3-100_3":"883610547","Sample_N3-500_1":"557425188","Sample_N3-500_2":"573439517","Sample_N3-500_3":"548205939","Sample_WT2-100_1":"842848912","Sample_WT2-100_2":"774522424","Sample_WT2-100_3":"786993232","Sample_WT2-500_1":"521559925","Sample_WT2-500_2":"496577361","Sample_WT2-500_3":"506168992","Sample_WT2-500-IN_1":"556707863","Sample_WT2-500-IN_2":"573985027","Sample_WT2-500-IN_3":"537606678"},{"Metabolite":"Glutamic acid +1","Sample_1-100_1":"64731065","Sample_1-100_2":"64033748","Sample_1-100_3":"62963865","Sample_1-500_1":"40840901","Sample_1-500_2":"34560033","Sample_1-500_3":"34453834","Sample_2-100_1":"142515892","Sample_2-100_2":"140551558","Sample_2-100_3":"143829559","Sample_2-500_1":"99150907","Sample_2-500_2":"102575980","Sample_2-500_3":"104250948","Sample_KO1-100_1":"75069440","Sample_KO1-100_2":"69931271","Sample_KO1-100_3":"68945497","Sample_KO1-500_1":"76728760","Sample_KO1-500_2":"77506541","Sample_KO1-500_3":"81996006","Sample_KO2-100_1":"113182291","Sample_KO2-100_2":"114256911","Sample_KO2-100_3":"109109033","Sample_KO2-500_1":"105120960","Sample_KO2-500_2":"102515904","Sample_KO2-500_3":"98824393","Sample_KO2-500_IN_1":"103297411","Sample_KO2-500_IN_2":"94260050","Sample_KO2-500_IN_3":"95971253","Sample_N3-100_1":"136259323","Sample_N3-100_2":"128817527","Sample_N3-100_3":"125846386","Sample_N3-500_1":"116889981","Sample_N3-500_2":"119625083","Sample_N3-500_3":"114881151","Sample_WT2-100_1":"134239275","Sample_WT2-100_2":"123769364","Sample_WT2-100_3":"124333864","Sample_WT2-500_1":"93785471","Sample_WT2-500_2":"89393240","Sample_WT2-500_3":"91389490","Sample_WT2-500-IN_1":"117220512","Sample_WT2-500-IN_2":"121170659","Sample_WT2-500-IN_3":"112838392"},{"Metabolite":"Glutamic acid +2","Sample_1-100_1":"146119761","Sample_1-100_2":"145026551","Sample_1-100_3":"141697707","Sample_1-500_1":"241705677","Sample_1-500_2":"202769601","Sample_1-500_3":"202165141","Sample_2-100_1":"229194743","Sample_2-100_2":"224700117","Sample_2-100_3":"228881377","Sample_2-500_1":"428100001","Sample_2-500_2":"441749984","Sample_2-500_3":"446347242","Sample_KO1-100_1":"162858637","Sample_KO1-100_2":"152544635","Sample_KO1-100_3":"153722433","Sample_KO1-500_1":"425237749","Sample_KO1-500_2":"430215487","Sample_KO1-500_3":"453421981","Sample_KO2-100_1":"229157096","Sample_KO2-100_2":"230172659","Sample_KO2-100_3":"220780715","Sample_KO2-500_1":"418368374","Sample_KO2-500_2":"408749868","Sample_KO2-500_3":"395104217","Sample_KO2-500_IN_1":"395891495","Sample_KO2-500_IN_2":"357052369","Sample_KO2-500_IN_3":"365876720","Sample_N3-100_1":"182728492","Sample_N3-100_2":"170007120","Sample_N3-100_3":"166708742","Sample_N3-500_1":"281672768","Sample_N3-500_2":"291581376","Sample_N3-500_3":"277371038","Sample_WT2-100_1":"264565017","Sample_WT2-100_2":"242956312","Sample_WT2-100_3":"248577781","Sample_WT2-500_1":"432668918","Sample_WT2-500_2":"410542950","Sample_WT2-500_3":"419680385","Sample_WT2-500-IN_1":"476549803","Sample_WT2-500-IN_2":"490745226","Sample_WT2-500-IN_3":"460883400"},{"Metabolite":"Glutamic acid +3","Sample_1-100_1":"28775717","Sample_1-100_2":"28547057","Sample_1-100_3":"27919693","Sample_1-500_1":"108794249","Sample_1-500_2":"90592850","Sample_1-500_3":"91211917","Sample_2-100_1":"49836428","Sample_2-100_2":"49218234","Sample_2-100_3":"50632230","Sample_2-500_1":"232442788","Sample_2-500_2":"240208289","Sample_2-500_3":"240655733","Sample_KO1-100_1":"18978655","Sample_KO1-100_2":"17941076","Sample_KO1-100_3":"17586699","Sample_KO1-500_1":"139025485","Sample_KO1-500_2":"139735941","Sample_KO1-500_3":"149372474","Sample_KO2-100_1":"51094524","Sample_KO2-100_2":"51831614","Sample_KO2-100_3":"49813698","Sample_KO2-500_1":"245922937","Sample_KO2-500_2":"240742165","Sample_KO2-500_3":"232627128","Sample_KO2-500_IN_1":"242582188","Sample_KO2-500_IN_2":"220605896","Sample_KO2-500_IN_3":"225426187","Sample_N3-100_1":"30791358","Sample_N3-100_2":"29132358","Sample_N3-100_3":"28458650","Sample_N3-500_1":"166866212","Sample_N3-500_2":"172406910","Sample_N3-500_3":"164995021","Sample_WT2-100_1":"57958707","Sample_WT2-100_2":"53526549","Sample_WT2-100_3":"54670307","Sample_WT2-500_1":"272158609","Sample_WT2-500_2":"259170516","Sample_WT2-500_3":"264848072","Sample_WT2-500-IN_1":"287674751","Sample_WT2-500-IN_2":"294749998","Sample_WT2-500-IN_3":"275421047"},{"Metabolite":"Glutamic acid +4","Sample_1-100_1":"17713748","Sample_1-100_2":"17389439","Sample_1-100_3":"17125746","Sample_1-500_1":"141116435","Sample_1-500_2":"118651391","Sample_1-500_3":"118177306","Sample_2-100_1":"24639221","Sample_2-100_2":"24403863","Sample_2-100_3":"25138721","Sample_2-500_1":"296076626","Sample_2-500_2":"305059386","Sample_2-500_3":"310941408","Sample_KO1-100_1":"9961391","Sample_KO1-100_2":"9691770","Sample_KO1-100_3":"8910523","Sample_KO1-500_1":"153681867","Sample_KO1-500_2":"156004007","Sample_KO1-500_3":"165811991","Sample_KO2-100_1":"29272291","Sample_KO2-100_2":"30098371","Sample_KO2-100_3":"28512795","Sample_KO2-500_1":"307023458","Sample_KO2-500_2":"299479255","Sample_KO2-500_3":"288755721","Sample_KO2-500_IN_1":"300374317","Sample_KO2-500_IN_2":"271478750","Sample_KO2-500_IN_3":"277589323","Sample_N3-100_1":"12484306","Sample_N3-100_2":"11228802","Sample_N3-100_3":"11394814","Sample_N3-500_1":"156748615","Sample_N3-500_2":"161197023","Sample_N3-500_3":"154300165","Sample_WT2-100_1":"33953585","Sample_WT2-100_2":"31068070","Sample_WT2-100_3":"31401558","Sample_WT2-500_1":"384674647","Sample_WT2-500_2":"366190144","Sample_WT2-500_3":"371472798","Sample_WT2-500-IN_1":"354508599","Sample_WT2-500-IN_2":"364856765","Sample_WT2-500-IN_3":"341677903"},{"Metabolite":"Glutamic acid +5","Sample_1-100_1":"2107561","Sample_1-100_2":"2079499","Sample_1-100_3":"2118887","Sample_1-500_1":"47447668","Sample_1-500_2":"40215542","Sample_1-500_3":"39536657","Sample_2-100_1":"3679007","Sample_2-100_2":"3651905","Sample_2-100_3":"3702859","Sample_2-500_1":"161834954","Sample_2-500_2":"166055488","Sample_2-500_3":"168097979","Sample_KO1-100_1":"639826","Sample_KO1-100_2":"672645","Sample_KO1-100_3":"639378","Sample_KO1-500_1":"29994419","Sample_KO1-500_2":"30424944","Sample_KO1-500_3":"32225285","Sample_KO2-100_1":"3930565","Sample_KO2-100_2":"3929399","Sample_KO2-100_3":"3861359","Sample_KO2-500_1":"149213206","Sample_KO2-500_2":"146006067","Sample_KO2-500_3":"140941926","Sample_KO2-500_IN_1":"156654249","Sample_KO2-500_IN_2":"142052518","Sample_KO2-500_IN_3":"145302140","Sample_N3-100_1":"1472620","Sample_N3-100_2":"1417444","Sample_N3-100_3":"1275593","Sample_N3-500_1":"87997979","Sample_N3-500_2":"89457891","Sample_N3-500_3":"85996125","Sample_WT2-100_1":"5202572","Sample_WT2-100_2":"4768249","Sample_WT2-100_3":"5087316","Sample_WT2-500_1":"235155502","Sample_WT2-500_2":"224761347","Sample_WT2-500_3":"228073404","Sample_WT2-500-IN_1":"181291678","Sample_WT2-500-IN_2":"187332059","Sample_WT2-500-IN_3":"174243428"},{"Metabolite":"Malic Acid","Sample_1-100_1":"21210911","Sample_1-100_2":"22661716","Sample_1-100_3":"21639114","Sample_1-500_1":"11475994","Sample_1-500_2":"10456354","Sample_1-500_3":"10386552","Sample_2-100_1":"48969219","Sample_2-100_2":"45012731","Sample_2-100_3":"49546364","Sample_2-500_1":"32644112","Sample_2-500_2":"36057921","Sample_2-500_3":"34812926","Sample_KO1-100_1":"34067699","Sample_KO1-100_2":"30826455","Sample_KO1-100_3":"30312891","Sample_KO1-500_1":"24624483","Sample_KO1-500_2":"22499364","Sample_KO1-500_3":"26044301","Sample_KO2-100_1":"20397324","Sample_KO2-100_2":"20101199","Sample_KO2-100_3":"21440448","Sample_KO2-500_1":"14218958","Sample_KO2-500_2":"13145230","Sample_KO2-500_3":"12879555","Sample_KO2-500_IN_1":"12595569","Sample_KO2-500_IN_2":"12470751","Sample_KO2-500_IN_3":"13264909","Sample_N3-100_1":"67201871","Sample_N3-100_2":"68123310","Sample_N3-100_3":"65228783","Sample_N3-500_1":"40202353","Sample_N3-500_2":"39405223","Sample_N3-500_3":"39129868","Sample_WT2-100_1":"25190009","Sample_WT2-100_2":"25248945","Sample_WT2-100_3":"22440808","Sample_WT2-500_1":"13626943","Sample_WT2-500_2":"13269445","Sample_WT2-500_3":"12885269","Sample_WT2-500-IN_1":"17540425","Sample_WT2-500-IN_2":"16922417","Sample_WT2-500-IN_3":"16540587"},{"Metabolite":"Malic Acid +1","Sample_1-100_1":"2546059","Sample_1-100_2":"2657854","Sample_1-100_3":"2240746","Sample_1-500_1":"2086676","Sample_1-500_2":"1822722","Sample_1-500_3":"1701177","Sample_2-100_1":"7352626","Sample_2-100_2":"6742489","Sample_2-100_3":"7396704","Sample_2-500_1":"6496733","Sample_2-500_2":"7249159","Sample_2-500_3":"7380622","Sample_KO1-100_1":"2439425","Sample_KO1-100_2":"2244082","Sample_KO1-100_3":"1941838","Sample_KO1-500_1":"3066921","Sample_KO1-500_2":"2996627","Sample_KO1-500_3":"3244652","Sample_KO2-100_1":"2697092","Sample_KO2-100_2":"2643792","Sample_KO2-100_3":"2777105","Sample_KO2-500_1":"2911794","Sample_KO2-500_2":"2725209","Sample_KO2-500_3":"2624240","Sample_KO2-500_IN_1":"2674956","Sample_KO2-500_IN_2":"2504026","Sample_KO2-500_IN_3":"2703439","Sample_N3-100_1":"8302219","Sample_N3-100_2":"8123769","Sample_N3-100_3":"8153223","Sample_N3-500_1":"10695781","Sample_N3-500_2":"10009819","Sample_N3-500_3":"9850016","Sample_WT2-100_1":"3293892","Sample_WT2-100_2":"3214989","Sample_WT2-100_3":"2745069","Sample_WT2-500_1":"2968412","Sample_WT2-500_2":"2554839","Sample_WT2-500_3":"2564959","Sample_WT2-500-IN_1":"3913490","Sample_WT2-500-IN_2":"3819812","Sample_WT2-500-IN_3":"3665406"},{"Metabolite":"Malic Acid +2","Sample_1-100_1":"4283542","Sample_1-100_2":"4212206","Sample_1-100_3":"3822815","Sample_1-500_1":"6623949","Sample_1-500_2":"5636034","Sample_1-500_3":"5735135","Sample_2-100_1":"8628152","Sample_2-100_2":"7876801","Sample_2-100_3":"8366209","Sample_2-500_1":"17512771","Sample_2-500_2":"19997842","Sample_2-500_3":"19012970","Sample_KO1-100_1":"3903524","Sample_KO1-100_2":"3288566","Sample_KO1-100_3":"3278450","Sample_KO1-500_1":"9461077","Sample_KO1-500_2":"9128353","Sample_KO1-500_3":"9868579","Sample_KO2-100_1":"3793041","Sample_KO2-100_2":"3935313","Sample_KO2-100_3":"3645372","Sample_KO2-500_1":"6633427","Sample_KO2-500_2":"5956091","Sample_KO2-500_3":"5939458","Sample_KO2-500_IN_1":"5727753","Sample_KO2-500_IN_2":"5495102","Sample_KO2-500_IN_3":"5546311","Sample_N3-100_1":"7643570","Sample_N3-100_2":"7884370","Sample_N3-100_3":"7459413","Sample_N3-500_1":"15818546","Sample_N3-500_2":"15443399","Sample_N3-500_3":"15442997","Sample_WT2-100_1":"4603750","Sample_WT2-100_2":"4239304","Sample_WT2-100_3":"3618398","Sample_WT2-500_1":"7475568","Sample_WT2-500_2":"6799094","Sample_WT2-500_3":"7004058","Sample_WT2-500-IN_1":"9347983","Sample_WT2-500-IN_2":"8607559","Sample_WT2-500-IN_3":"8029345"},{"Metabolite":"Malic Acid +3","Sample_1-100_1":"1026433","Sample_1-100_2":"856385","Sample_1-100_3":"1013252","Sample_1-500_1":"4991552","Sample_1-500_2":"4413956","Sample_1-500_3":"4068851","Sample_2-100_1":"2177583","Sample_2-100_2":"2094990","Sample_2-100_3":"2244228","Sample_2-500_1":"15172827","Sample_2-500_2":"16751704","Sample_2-500_3":"16363805","Sample_KO1-100_1":"387014","Sample_KO1-100_2":"281534","Sample_KO1-100_3":"244099","Sample_KO1-500_1":"4554275","Sample_KO1-500_2":"4250832","Sample_KO1-500_3":"4644165","Sample_KO2-100_1":"887703","Sample_KO2-100_2":"898102","Sample_KO2-100_3":"859024","Sample_KO2-500_1":"5329790","Sample_KO2-500_2":"5211141","Sample_KO2-500_3":"4902814","Sample_KO2-500_IN_1":"4716276","Sample_KO2-500_IN_2":"4813546","Sample_KO2-500_IN_3":"5053966","Sample_N3-100_1":"1314796","Sample_N3-100_2":"1316489","Sample_N3-100_3":"1218596","Sample_N3-500_1":"12099856","Sample_N3-500_2":"12117893","Sample_N3-500_3":"11363154","Sample_WT2-100_1":"1035406","Sample_WT2-100_2":"1169555","Sample_WT2-100_3":"884354","Sample_WT2-500_1":"6960220","Sample_WT2-500_2":"6514674","Sample_WT2-500_3":"6567372","Sample_WT2-500-IN_1":"7626086","Sample_WT2-500-IN_2":"7604532","Sample_WT2-500-IN_3":"6907872"},{"Metabolite":"Malic Acid +4","Sample_1-100_1":"0","Sample_1-100_2":"0","Sample_1-100_3":"0","Sample_1-500_1":"1606516","Sample_1-500_2":"1279600","Sample_1-500_3":"1201508","Sample_2-100_1":"195166","Sample_2-100_2":"133006","Sample_2-100_3":"185813","Sample_2-500_1":"7240285","Sample_2-500_2":"8395875","Sample_2-500_3":"8188979","Sample_KO1-100_1":"0","Sample_KO1-100_2":"0","Sample_KO1-100_3":"0","Sample_KO1-500_1":"710800","Sample_KO1-500_2":"816463","Sample_KO1-500_3":"924355","Sample_KO2-100_1":"0","Sample_KO2-100_2":"0","Sample_KO2-100_3":"0","Sample_KO2-500_1":"2023915","Sample_KO2-500_2":"1875018","Sample_KO2-500_3":"1982871","Sample_KO2-500_IN_1":"2327213","Sample_KO2-500_IN_2":"2011710","Sample_KO2-500_IN_3":"2048920","Sample_N3-100_1":"0","Sample_N3-100_2":"0","Sample_N3-100_3":"0","Sample_N3-500_1":"5331941","Sample_N3-500_2":"4977635","Sample_N3-500_3":"5305274","Sample_WT2-100_1":"0","Sample_WT2-100_2":"0","Sample_WT2-100_3":"0","Sample_WT2-500_1":"3628734","Sample_WT2-500_2":"3280978","Sample_WT2-500_3":"3277500","Sample_WT2-500-IN_1":"3084047","Sample_WT2-500-IN_2":"3201285","Sample_WT2-500-IN_3":"2931704"},{"Metabolite":"Palmitic acid","Sample_1-100_1":"1284155540","Sample_1-100_2":"1299963847","Sample_1-100_3":"1364778616","Sample_1-500_1":"1366130660","Sample_1-500_2":"1405327113","Sample_1-500_3":"1428422321","Sample_2-100_1":"1207383134","Sample_2-100_2":"1129856642","Sample_2-100_3":"1275765194","Sample_2-500_1":"1237203955","Sample_2-500_2":"1254987428","Sample_2-500_3":"1225049781","Sample_KO1-100_1":"888740161","Sample_KO1-100_2":"946520594","Sample_KO1-100_3":"916821287","Sample_KO1-500_1":"707426293","Sample_KO1-500_2":"746991177","Sample_KO1-500_3":"730293505","Sample_KO2-100_1":"803866168","Sample_KO2-100_2":"780790931","Sample_KO2-100_3":"793302553","Sample_KO2-500_1":"848075142","Sample_KO2-500_2":"866202214","Sample_KO2-500_3":"950716110","Sample_KO2-500_IN_1":"856601586","Sample_KO2-500_IN_2":"890835996","Sample_KO2-500_IN_3":"898770799","Sample_N3-100_1":"1163527158","Sample_N3-100_2":"1244400860","Sample_N3-100_3":"1247677309","Sample_N3-500_1":"1129445100","Sample_N3-500_2":"1142573278","Sample_N3-500_3":"1156909267","Sample_WT2-100_1":"762713560","Sample_WT2-100_2":"851112925","Sample_WT2-100_3":"882342149","Sample_WT2-500_1":"853287319","Sample_WT2-500_2":"934408941","Sample_WT2-500_3":"993227464","Sample_WT2-500-IN_1":"945618969","Sample_WT2-500-IN_2":"1040527748","Sample_WT2-500-IN_3":"1184905358"},{"Metabolite":"Palmitic acid +2","Sample_1-100_1":"17473993","Sample_1-100_2":"18188077","Sample_1-100_3":"19141124","Sample_1-500_1":"18940198","Sample_1-500_2":"19422104","Sample_1-500_3":"19099628","Sample_2-100_1":"16193761","Sample_2-100_2":"15979848","Sample_2-100_3":"16157605","Sample_2-500_1":"16592753","Sample_2-500_2":"16117721","Sample_2-500_3":"16928892","Sample_KO1-100_1":"12119943","Sample_KO1-100_2":"12738883","Sample_KO1-100_3":"11936257","Sample_KO1-500_1":"10132483","Sample_KO1-500_2":"8249164","Sample_KO1-500_3":"10274200","Sample_KO2-100_1":"10116156","Sample_KO2-100_2":"10152358","Sample_KO2-100_3":"10110018","Sample_KO2-500_1":"11726297","Sample_KO2-500_2":"11714822","Sample_KO2-500_3":"12540615","Sample_KO2-500_IN_1":"11624682","Sample_KO2-500_IN_2":"11486342","Sample_KO2-500_IN_3":"12570296","Sample_N3-100_1":"15658725","Sample_N3-100_2":"16645433","Sample_N3-100_3":"16639281","Sample_N3-500_1":"15365631","Sample_N3-500_2":"16264704","Sample_N3-500_3":"16815773","Sample_WT2-100_1":"10626361","Sample_WT2-100_2":"10804510","Sample_WT2-100_3":"11011420","Sample_WT2-500_1":"11871808","Sample_WT2-500_2":"13070401","Sample_WT2-500_3":"13149591","Sample_WT2-500-IN_1":"13955418","Sample_WT2-500-IN_2":"12006497","Sample_WT2-500-IN_3":"15572796"},{"Metabolite":"Palmitic acid +4","Sample_1-100_1":"0","Sample_1-100_2":"0","Sample_1-100_3":"0","Sample_1-500_1":"0","Sample_1-500_2":"0","Sample_1-500_3":"0","Sample_2-100_1":"0","Sample_2-100_2":"0","Sample_2-100_3":"0","Sample_2-500_1":"0","Sample_2-500_2":"0","Sample_2-500_3":"0","Sample_KO1-100_1":"0","Sample_KO1-100_2":"0","Sample_KO1-100_3":"0","Sample_KO1-500_1":"0","Sample_KO1-500_2":"0","Sample_KO1-500_3":"0","Sample_KO2-100_1":"0","Sample_KO2-100_2":"0","Sample_KO2-100_3":"0","Sample_KO2-500_1":"0","Sample_KO2-500_2":"0","Sample_KO2-500_3":"0","Sample_KO2-500_IN_1":"0","Sample_KO2-500_IN_2":"0","Sample_KO2-500_IN_3":"0","Sample_N3-100_1":"0","Sample_N3-100_2":"0","Sample_N3-100_3":"0","Sample_N3-500_1":"0","Sample_N3-500_2":"0","Sample_N3-500_3":"0","Sample_WT2-100_1":"0","Sample_WT2-100_2":"0","Sample_WT2-100_3":"0","Sample_WT2-500_1":"0","Sample_WT2-500_2":"0","Sample_WT2-500_3":"0","Sample_WT2-500-IN_1":"0","Sample_WT2-500-IN_2":"0","Sample_WT2-500-IN_3":"0"}],

"Metabolites":[{"Metabolite":"alpha-Ketoglutaric acid","Formula":"C5H6O5","m/z":"145.0143","RT (min)":"9.85","Adduct":"M-H"},{"Metabolite":"alpha-Ketoglutaric acid +1","Formula":"[C13]C4H6O5","m/z":"146.0176","RT (min)":"9.85","Adduct":"M-H"},{"Metabolite":"alpha-Ketoglutaric acid +2","Formula":"[C13]2C3H6O5","m/z":"147.021","RT (min)":"9.85","Adduct":"M-H"},{"Metabolite":"alpha-Ketoglutaric acid +3","Formula":"[C13]3C2H6O5","m/z":"148.0243","RT (min)":"9.85","Adduct":"M-H"},{"Metabolite":"alpha-Ketoglutaric acid +4","Formula":"[C13]4CH6O5","m/z":"149.0277","RT (min)":"9.85","Adduct":"M-H"},{"Metabolite":"alpha-Ketoglutaric acid +5","Formula":"[C13]5H6O5","m/z":"150.031","RT (min)":"9.85","Adduct":"M-H"},{"Metabolite":"Citric acid","Formula":"C6H8O7","m/z":"191.0197","RT (min)":"11.21","Adduct":"M-H"},{"Metabolite":"Citric acid +1","Formula":"[C13]C5H8O7","m/z":"192.0231","RT (min)":"11.21","Adduct":"M-H"},{"Metabolite":"Citric acid +2","Formula":"[C13]2C4H8O7","m/z":"193.0264","RT (min)":"11.21","Adduct":"M-H"},{"Metabolite":"Citric acid +3","Formula":"[C13]3C3H8O7","m/z":"194.0298","RT (min)":"11.21","Adduct":"M-H"},{"Metabolite":"Citric acid +4","Formula":"[C13]4C2H8O7","m/z":"195.0332","RT (min)":"11.21","Adduct":"M-H"},{"Metabolite":"Citric acid +5","Formula":"[C13]5C1H8O7","m/z":"196.0365","RT (min)":"11.21","Adduct":"M-H"},{"Metabolite":"Citric acid +6","Formula":"[C13]6H8O7","m/z":"197.0399","RT (min)":"11.21","Adduct":"M-H"},{"Metabolite":"Glutamic acid","Formula":"C5H9NO4","m/z":"148.0604","RT (min)":"9.37","Adduct":"M+H"},{"Metabolite":"Glutamic acid +1","Formula":"[C13]C4H9NO4","m/z":"149.0638","RT (min)":"9.37","Adduct":"M+H"},{"Metabolite":"Glutamic acid +2","Formula":"[C13]2C3H9NO4","m/z":"150.0671","RT (min)":"9.37","Adduct":"M+H"},{"Metabolite":"Glutamic acid +3","Formula":"[C13]3C2H9NO4","m/z":"151.0705","RT (min)":"9.37","Adduct":"M+H"},{"Metabolite":"Glutamic acid +4","Formula":"[C13]4CH9NO4","m/z":"152.0739","RT (min)":"9.37","Adduct":"M+H"},{"Metabolite":"Glutamic acid +5","Formula":"[C13]5H9NO4","m/z":"153.0772","RT (min)":"9.37","Adduct":"M+H"},{"Metabolite":"Malic Acid","Formula":"C4H6O5","m/z":"133.0143","RT (min)":"10.16","Adduct":"M-H"},{"Metabolite":"Malic Acid +1","Formula":"[C13]C3H6O5","m/z":"134.0176","RT (min)":"10.16","Adduct":"M-H"},{"Metabolite":"Malic Acid +2","Formula":"[C13]2C2H6O5","m/z":"135.021","RT (min)":"10.16","Adduct":"M-H"},{"Metabolite":"Malic Acid +3","Formula":"[C13]3CH6O5","m/z":"136.0243","RT (min)":"10.16","Adduct":"M-H"},{"Metabolite":"Malic Acid +4","Formula":"[C13]4H6O5","m/z":"137.0277","RT (min)":"10.16","Adduct":"M-H"},{"Metabolite":"Palmitic acid","Formula":"C16H32O2","m/z":"255.233","RT (min)":"1.31","Adduct":"M-H"},{"Metabolite":"Palmitic acid +2","Formula":"[C13]2C14H32O2","m/z":"257.2397","RT (min)":"1.31","Adduct":"M-H"},{"Metabolite":"Palmitic acid +4","Formula":"[C13]4C12H32O2","m/z":"259.2464","RT (min)":"1.31","Adduct":"M-H"}]
}

}