{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002743","ANALYSIS_ID":"AN004448","VERSION":"1","CREATED_ON":"June 26, 2023, 10:29 am"}, "PROJECT":{"PROJECT_TITLE":"Metabolomics comparison of lung fibroblasts from Pteropus alecto and Homo sapiens","PROJECT_TYPE":"MS quantitative analysis","PROJECT_SUMMARY":"Targeted Metabolomics was performed to identify differences in central carbon metabolism between lung fibroblasts from black flying fox fruit bat Pteropus alecto (PaLung cells) and Humans (WI-38 cells).","INSTITUTE":"Duke-NUS Medical School","LAST_NAME":"Jagannathan","FIRST_NAME":"Narendra Suhas","ADDRESS":"Centre for Computational Biology, 8 College Road, Singapore, Singapore, 169857, Singapore","EMAIL":"gmsnsja@nus.edu.sg","PHONE":"(65) 66015073","PROJECT_COMMENTS":"Metabolomics was performed by commissioning to Human Metabolome Technologies (HMT) service.","CONTRIBUTORS":"Javier Yu Peng Koh, Yoko Itahana, Koji Itahana, Lisa Tucker-Kellogg"}, "STUDY":{"STUDY_TITLE":"Metabolomics comparison of lung fibroblasts from Pteropus alecto and Homo sapiens","STUDY_TYPE":"MS Quantitative analysis","STUDY_SUMMARY":"Targeted Metabolomics was performed to identify differences in central carbon metabolism between lung fibroblasts from black flying fox fruit bat Pteropus alecto (PaLung cells) and Humans (WI-38 cells). For both PaLung and WI-38 cells, metabolomics measurements were carried out for n = 3 biological replicates.","INSTITUTE":"Duke-NUS Medical School","LAST_NAME":"Jagannathan","FIRST_NAME":"Narendra Suhas","ADDRESS":"8 College Road, Singapore, Singapore, 169857, Singapore","EMAIL":"gmsnsja@nus.edu.sg","PHONE":"(65) 66015073"}, "SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Pteropus alecto / Homo sapiens"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"WI38-1", "Factors":{"Genotype":"Wild-type","Species":"H. sapiens"} }, { "Subject ID":"-", "Sample ID":"WI38-2", "Factors":{"Genotype":"Wild-type","Species":"H. sapiens"} }, { "Subject ID":"-", "Sample ID":"WI38-3", "Factors":{"Genotype":"Wild-type","Species":"H. sapiens"} }, { "Subject ID":"-", "Sample ID":"PaLung-1", "Factors":{"Genotype":"Wild-type","Species":"P. alecto"} }, { "Subject ID":"-", "Sample ID":"PaLung-2", "Factors":{"Genotype":"Wild-type","Species":"P. alecto"} }, { "Subject ID":"-", "Sample ID":"PaLung-3", "Factors":{"Genotype":"Wild-type","Species":"P. alecto"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"WI-38 and PaLung cells were seeded in a 100 mm dish and harvested 2 days after seeding according to the protocol provided by Human Metabolome Technologies (HMT Japan).","SAMPLE_TYPE":"Cultured fibroblasts"}, "TREATMENT":{"TREATMENT_SUMMARY":"PaLung and WI-38 cells were washed with 5% (w/w) mannitol twice and fixed with methanol containing the internal standard solution. The internal standard solution was provided by HMT. Lysates were centrifuged at 2,300 x g for 5 min at 4°C, and supernatants were filtered using the provided filter units by centrifuging at 9,100 × g for 3 hours at 4 °C. Filtrates were evaporated by SpeedVac Concentrator SPD1010 (Thermo Fisher Scientific) and submitted to HMT for analysis. The total cell numbers at the time of harvest were about 1.4 x 107 cells for each cell line. Targeted quantitative analysis was performed by HMT using capillary electrophoresis mass spectrometry (CE-TOFMS and CE-QqQMS). Absolute abundances (normalized by cell numbers) were obtained for a total of 116 metabolites (54 and 62 metabolites in the cation and anion modes, respectively)."}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"PaLung and WI-38 cells were washed with 5% (w/w) mannitol twice and fixed with methanol containing the internal standard solution. The internal standard solution was provided by HMT. Lysates were centrifuged at 2,300 x g for 5 min at 4°C, and supernatants were filtered using the provided filter units by centrifuging at 9,100 × g for 3 hours at 4 °C. Filtrates were evaporated by SpeedVac Concentrator SPD1010 (Thermo Fisher Scientific) and submitted to HMT for analysis. The total cell numbers at the time of harvest were about 1.4 x 107 cells for each cell line. Targeted quantitative analysis was performed by HMT using capillary electrophoresis mass spectrometry (CE-TOFMS and CE-QqQMS). Absolute abundances (normalized by cell numbers) were obtained for a total of 116 metabolites (54 and 62 metabolites in the cation and anion modes, respectively)."}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"CE","INSTRUMENT_NAME":"Agilent CE","COLUMN_NAME":"Fused silica capillary, i.d. 50 μm × 80 cm","SOLVENT_A":"NA","SOLVENT_B":"NA","FLOW_GRADIENT":"NA","FLOW_RATE":"NA","COLUMN_TEMPERATURE":"NA"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_NAME":"Agilent 6460 QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Peaks detected in CE-TOFMS analysis were extracted using automatic integration software (MasterHands ver.2.18.0.1 developed at Keio University) (4) and those in CE-QqQMS analysis were extracted using automatic integration software (MassHunter Quantitative Analysis B.06.00 Agilent Technologies, Santa Clara, CA, USA) in order to obtain peak information, which includes m/z, migration time (MT), and peak area."}, "MS_METABOLITE_DATA":{ "Units":"pmol/10^6 cells", "Data":[{"Metabolite":"NAD+","WI38-1":"2352.3","WI38-2":"2395.1","WI38-3":"2493.1","PaLung-1":"1198.4","PaLung-2":"1233.3","PaLung-3":"1114.4"},{"Metabolite":"cAMP","WI38-1":"4.0","WI38-2":"4.7","WI38-3":"4.2","PaLung-1":"3.1","PaLung-2":"2.2","PaLung-3":"3.2"},{"Metabolite":"cGMP","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"NADH","WI38-1":"83.7","WI38-2":"79.8","WI38-3":"82.9","PaLung-1":"141.1","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"Xanthine","WI38-1":"47.7","WI38-2":"39.5","WI38-3":"44.3","PaLung-1":"34.1","PaLung-2":"33.8","PaLung-3":"28.4"},{"Metabolite":"ADP-ribose","WI38-1":"59.7","WI38-2":"59.4","WI38-3":"58.9","PaLung-1":"42.2","PaLung-2":"58.3","PaLung-3":"53.2"},{"Metabolite":"Mevalonic acid","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"UDP-glucose","WI38-1":"1814.4","WI38-2":"1851.3","WI38-3":"1700.4","PaLung-1":"1543.9","PaLung-2":"1581.4","PaLung-3":"1627.8"},{"Metabolite":"Uric acid","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"NADP+","WI38-1":"49.2","WI38-2":"51.5","WI38-3":"53.8","PaLung-1":"67.2","PaLung-2":"57.1","PaLung-3":"60.3"},{"Metabolite":"IMP","WI38-1":"19.5","WI38-2":"22.0","WI38-3":"18.3","PaLung-1":"450.7","PaLung-2":"626.1","PaLung-3":"633.9"},{"Metabolite":"Sedoheptulose 7-phosphate","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"Glucose 6-phosphate","WI38-1":"677.8","WI38-2":"645.1","WI38-3":"532.4","PaLung-1":"109.9","PaLung-2":"94.6","PaLung-3":"93.9"},{"Metabolite":"Fructose 6-phosphate","WI38-1":"206.6","WI38-2":"186.9","WI38-3":"174.5","PaLung-1":"139.1","PaLung-2":"112.4","PaLung-3":"131.8"},{"Metabolite":"Fructose 1-phosphate","WI38-1":"49.3","WI38-2":"50.0","WI38-3":"33.9","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"Galactose 1-phosphate","WI38-1":"39.7","WI38-2":"28.7","WI38-3":"26.7","PaLung-1":"16.4","PaLung-2":"13.8","PaLung-3":"14.8"},{"Metabolite":"Glucose 1-phosphate","WI38-1":"48.7","WI38-2":"47.5","WI38-3":"34.0","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"Acetoacetyl CoA","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"Acetyl CoA","WI38-1":"29.1","WI38-2":"24.8","WI38-3":"26.0","PaLung-1":"18.0","PaLung-2":"17.7","PaLung-3":"22.0"},{"Metabolite":"Folic acid","WI38-1":"1.9","WI38-2":"2.0","WI38-3":"2.1","PaLung-1":"3.1","PaLung-2":"2.3","PaLung-3":"1.8"},{"Metabolite":"Ribose 5-phosphate","WI38-1":"91.6","WI38-2":"84.5","WI38-3":"68.3","PaLung-1":"72.8","PaLung-2":"65.2","PaLung-3":"65.5"},{"Metabolite":"CoA","WI38-1":"8.1","WI38-2":"7.3","WI38-3":"7.1","PaLung-1":"11.6","PaLung-2":"11.1","PaLung-3":"10.9"},{"Metabolite":"Ribose 1-phosphate","WI38-1":"16.9","WI38-2":"27.9","WI38-3":"32.4","PaLung-1":"21.8","PaLung-2":"23.3","PaLung-3":"27.9"},{"Metabolite":"Ribulose 5-phosphate","WI38-1":"73.1","WI38-2":"75.3","WI38-3":"75.3","PaLung-1":"54.6","PaLung-2":"47.7","PaLung-3":"46.5"},{"Metabolite":"Xylulose 5-phosphate","WI38-1":"83.6","WI38-2":"64.8","WI38-3":"60.0","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"Erythrose 4-phosphate","WI38-1":"15.8","WI38-2":"21.6","WI38-3":"21.9","PaLung-1":"18.3","PaLung-2":"20.1","PaLung-3":"17.8"},{"Metabolite":"HMG CoA","WI38-1":"6.7","WI38-2":"5.9","WI38-3":"5.8","PaLung-1":"10.1","PaLung-2":"9.3","PaLung-3":"10.3"},{"Metabolite":"Glyceraldehyde 3-phosphate","WI38-1":"245.6","WI38-2":"200.3","WI38-3":"178.4","PaLung-1":"552.2","PaLung-2":"713.4","PaLung-3":"624.6"},{"Metabolite":"NADPH","WI38-1":"N.D.","WI38-2":"53.2","WI38-3":"56.3","PaLung-1":"95.5","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"Malonyl CoA","WI38-1":"6.4","WI38-2":"7.8","WI38-3":"8.5","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"Phosphocreatine","WI38-1":"491.7","WI38-2":"401.2","WI38-3":"300.7","PaLung-1":"758.5","PaLung-2":"620.9","PaLung-3":"822.5"},{"Metabolite":"XMP","WI38-1":"3.3","WI38-2":"2.9","WI38-3":"3.1","PaLung-1":"3.9","PaLung-2":"3.5","PaLung-3":"6.4"},{"Metabolite":"Dihydroxyacetone phosphate","WI38-1":"1353.0","WI38-2":"1247.5","WI38-3":"1094.4","PaLung-1":"1653.0","PaLung-2":"1552.8","PaLung-3":"1543.0"},{"Metabolite":"Adenylosuccinic acid","WI38-1":"3.9","WI38-2":"4.0","WI38-3":"4.0","PaLung-1":"13.3","PaLung-2":"14.1","PaLung-3":"15.5"},{"Metabolite":"Fructose 1,6-diphosphate","WI38-1":"3937.2","WI38-2":"3916.2","WI38-3":"3681.5","PaLung-1":"5263.5","PaLung-2":"5740.5","PaLung-3":"5406.5"},{"Metabolite":"6-Phosphogluconic acid","WI38-1":"232.5","WI38-2":"219.9","WI38-3":"194.5","PaLung-1":"76.2","PaLung-2":"61.6","PaLung-3":"55.9"},{"Metabolite":"N-Carbamoylaspartic acid","WI38-1":"25.6","WI38-2":"22.1","WI38-3":"20.6","PaLung-1":"10.0","PaLung-2":"12.1","PaLung-3":"9.8"},{"Metabolite":"PRPP","WI38-1":"68.3","WI38-2":"64.5","WI38-3":"65.6","PaLung-1":"39.9","PaLung-2":"37.2","PaLung-3":"37.3"},{"Metabolite":"2-Phosphoglyceric acid","WI38-1":"17.1","WI38-2":"19.1","WI38-3":"21.7","PaLung-1":"20.7","PaLung-2":"24.8","PaLung-3":"19.4"},{"Metabolite":"2,3-Diphosphoglyceric acid","WI38-1":"455.6","WI38-2":"496.6","WI38-3":"485.1","PaLung-1":"1173.3","PaLung-2":"1261.9","PaLung-3":"1261.4"},{"Metabolite":"3-Phosphoglyceric acid","WI38-1":"186.5","WI38-2":"211.6","WI38-3":"261.9","PaLung-1":"243.8","PaLung-2":"207.2","PaLung-3":"200.5"},{"Metabolite":"Phosphoenolpyruvic acid","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"0.4","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"GMP","WI38-1":"25.8","WI38-2":"33.4","WI38-3":"26.1","PaLung-1":"85.1","PaLung-2":"88.8","PaLung-3":"92.6"},{"Metabolite":"AMP","WI38-1":"97.3","WI38-2":"94.4","WI38-3":"91.6","PaLung-1":"305.9","PaLung-2":"275.6","PaLung-3":"236.6"},{"Metabolite":"2-Oxoisovaleric acid","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"GDP","WI38-1":"113.4","WI38-2":"120.9","WI38-3":"126.8","PaLung-1":"199.2","PaLung-2":"185.3","PaLung-3":"154.1"},{"Metabolite":"Lactic acid","WI38-1":"25643.1","WI38-2":"24251.0","WI38-3":"27589.3","PaLung-1":"12548.4","PaLung-2":"11242.2","PaLung-3":"10670.2"},{"Metabolite":"ADP","WI38-1":"665.6","WI38-2":"693.4","WI38-3":"776.7","PaLung-1":"1164.2","PaLung-2":"1128.9","PaLung-3":"1038.1"},{"Metabolite":"GTP","WI38-1":"2419.3","WI38-2":"2316.7","WI38-3":"2346.1","PaLung-1":"1210.6","PaLung-2":"1245.4","PaLung-3":"1241.1"},{"Metabolite":"Glyoxylic acid","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"ATP","WI38-1":"9143.2","WI38-2":"9287.4","WI38-3":"9368.9","PaLung-1":"3897.0","PaLung-2":"3711.5","PaLung-3":"3952.4"},{"Metabolite":"Glycerol 3-phosphate","WI38-1":"308.7","WI38-2":"276.1","WI38-3":"310.6","PaLung-1":"350.0","PaLung-2":"343.3","PaLung-3":"386.2"},{"Metabolite":"Glycolic acid","WI38-1":"N.D.","WI38-2":"100.51","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"391.61","PaLung-3":"272.07"},{"Metabolite":"Pyruvic acid","WI38-1":"231.18","WI38-2":"180.98","WI38-3":"201.04","PaLung-1":"292.33","PaLung-2":"292.64","PaLung-3":"318.94"},{"Metabolite":"N-Acetylglutamic acid","WI38-1":"25.70","WI38-2":"31.71","WI38-3":"30.08","PaLung-1":"27.62","PaLung-2":"19.69","PaLung-3":"19.61"},{"Metabolite":"2-Hydroxyglutaric acid","WI38-1":"39.89","WI38-2":"33.46","WI38-3":"39.89","PaLung-1":"50.07","PaLung-2":"55.38","PaLung-3":"52.45"},{"Metabolite":"Carbamoylphosphate","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"74.10"},{"Metabolite":"Succinic acid","WI38-1":"492.57","WI38-2":"478.27","WI38-3":"548.23","PaLung-1":"381.15","PaLung-2":"354.22","PaLung-3":"352.24"},{"Metabolite":"Malic acid","WI38-1":"2354.65","WI38-2":"2448.25","WI38-3":"2736.21","PaLung-1":"211.14","PaLung-2":"265.53","PaLung-3":"293.28"},{"Metabolite":"2-Oxoglutaric acid","WI38-1":"N.D.","WI38-2":"N.D.","WI38-3":"N.D.","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."},{"Metabolite":"Fumaric acid","WI38-1":"462.06","WI38-2":"452.50","WI38-3":"507.20","PaLung-1":"73.30","PaLung-2":"57.21","PaLung-3":"71.54"},{"Metabolite":"Citric acid","WI38-1":"2478.15","WI38-2":"2270.38","WI38-3":"2411.20","PaLung-1":"1339.78","PaLung-2":"1389.65","PaLung-3":"1332.35"},{"Metabolite":"cis-Aconitic acid","WI38-1":"57.65","WI38-2":"51.17","WI38-3":"53.06","PaLung-1":"24.50","PaLung-2":"18.19","PaLung-3":"18.61"},{"Metabolite":"Isocitric acid","WI38-1":"49.89","WI38-2":"63.22","WI38-3":"89.90","PaLung-1":"N.D.","PaLung-2":"N.D.","PaLung-3":"N.D."}], "Metabolites":[{"Metabolite":"NAD+","PubChem ID":"5893","HMDB ID":"HMDB0000902"},{"Metabolite":"cAMP","PubChem ID":"6076","HMDB ID":"HMDB0000058"},{"Metabolite":"cGMP","PubChem ID":"24316","HMDB ID":"HMDB0001314"},{"Metabolite":"NADH","PubChem ID":"439153","HMDB ID":"HMDB0001487"},{"Metabolite":"Xanthine","PubChem ID":"1188","HMDB ID":"HMDB0000292"},{"Metabolite":"ADP-ribose","PubChem ID":"445794","HMDB ID":"HMDB0001178"},{"Metabolite":"Mevalonic acid","PubChem ID":"134965","HMDB ID":"HMDB0000227"},{"Metabolite":"UDP-glucose","PubChem ID":"8629","HMDB ID":"HMDB0000286"},{"Metabolite":"Uric acid","PubChem ID":"1175","HMDB ID":"HMDB0000289"},{"Metabolite":"NADP+","PubChem ID":"5886","HMDB ID":"HMDB0000217"},{"Metabolite":"IMP","PubChem ID":"8582","HMDB ID":"HMDB0000175"},{"Metabolite":"Sedoheptulose 7-phosphate","PubChem ID":"165007","HMDB ID":"HMDB0001068"},{"Metabolite":"Glucose 6-phosphate","PubChem ID":"5958","HMDB ID":"HMDB0001401"},{"Metabolite":"Fructose 6-phosphate","PubChem ID":"603","HMDB ID":"HMDB0000124"},{"Metabolite":"Fructose 1-phosphate","PubChem ID":"439394","HMDB ID":"HMDB0001076"},{"Metabolite":"Galactose 1-phosphate","PubChem ID":"123912","HMDB ID":"HMDB0000645"},{"Metabolite":"Glucose 1-phosphate","PubChem ID":"65533","HMDB ID":"HMDB0001586"},{"Metabolite":"Acetoacetyl CoA","PubChem ID":"92153","HMDB ID":"HMDB0001484"},{"Metabolite":"Acetyl CoA","PubChem ID":"444493","HMDB ID":"HMDB0001206"},{"Metabolite":"Folic acid","PubChem ID":"6037","HMDB ID":"HMDB0000121"},{"Metabolite":"Ribose 5-phosphate","PubChem ID":"439167","HMDB ID":"HMDB0001548"},{"Metabolite":"CoA","PubChem ID":"87642","HMDB ID":"HMDB0001423"},{"Metabolite":"Ribose 1-phosphate","PubChem ID":"439236","HMDB ID":"HMDB0001489"},{"Metabolite":"Ribulose 5-phosphate","PubChem ID":"439184","HMDB ID":"HMDB0000618"},{"Metabolite":"Xylulose 5-phosphate","PubChem ID":"439190","HMDB ID":"HMDB0000868"},{"Metabolite":"Erythrose 4-phosphate","PubChem ID":"122357","HMDB ID":"HMDB0001321"},{"Metabolite":"HMG CoA","PubChem ID":"445127","HMDB ID":"HMDB0001375"},{"Metabolite":"Glyceraldehyde 3-phosphate","PubChem ID":"729","HMDB ID":"HMDB0001112"},{"Metabolite":"NADPH","PubChem ID":"5884","HMDB ID":"HMDB0000221"},{"Metabolite":"Malonyl CoA","PubChem ID":"644066","HMDB ID":"HMDB0001175"},{"Metabolite":"Phosphocreatine","PubChem ID":"9548602","HMDB ID":"HMDB0001511"},{"Metabolite":"XMP","PubChem ID":"73323","HMDB ID":"HMDB0001554"},{"Metabolite":"Dihydroxyacetone phosphate","PubChem ID":"668","HMDB ID":"HMDB0001473"},{"Metabolite":"Adenylosuccinic acid","PubChem ID":"447145","HMDB ID":"HMDB0000536"},{"Metabolite":"Fructose 1,6-diphosphate","PubChem ID":"172313","HMDB ID":"HMDB0001058"},{"Metabolite":"6-Phosphogluconic acid","PubChem ID":"91493","HMDB ID":"HMDB0001316"},{"Metabolite":"N-Carbamoylaspartic acid","PubChem ID":"93072","HMDB ID":"HMDB0000828"},{"Metabolite":"PRPP","PubChem ID":"7339","HMDB ID":"HMDB0000280"},{"Metabolite":"2-Phosphoglyceric acid","PubChem ID":"439278","HMDB ID":"HMDB0003391"},{"Metabolite":"2,3-Diphosphoglyceric acid","PubChem ID":"186004","HMDB ID":"HMDB0001294"},{"Metabolite":"3-Phosphoglyceric acid","PubChem ID":"439183","HMDB ID":"HMDB0000807"},{"Metabolite":"Phosphoenolpyruvic acid","PubChem ID":"1005","HMDB ID":"HMDB0000263"},{"Metabolite":"GMP","PubChem ID":"6804","HMDB ID":"HMDB0001397"},{"Metabolite":"AMP","PubChem ID":"6083","HMDB ID":"HMDB0000045"},{"Metabolite":"2-Oxoisovaleric acid","PubChem ID":"49","HMDB ID":"HMDB0000019"},{"Metabolite":"GDP","PubChem ID":"8977","HMDB ID":"HMDB0001201"},{"Metabolite":"Lactic acid","PubChem ID":"612","HMDB ID":"HMDB0000190,HMDB0001311"},{"Metabolite":"ADP","PubChem ID":"6022","HMDB ID":"HMDB0001341"},{"Metabolite":"GTP","PubChem ID":"6830","HMDB ID":"HMDB0001273"},{"Metabolite":"Glyoxylic acid","PubChem ID":"760","HMDB ID":"HMDB0000119"},{"Metabolite":"ATP","PubChem ID":"5957","HMDB ID":"HMDB0000538"},{"Metabolite":"Glycerol 3-phosphate","PubChem ID":"439162","HMDB ID":"HMDB0000126"},{"Metabolite":"Glycolic acid","PubChem ID":"757","HMDB ID":"HMDB0000115"},{"Metabolite":"Pyruvic acid","PubChem ID":"1060","HMDB ID":"HMDB0000243"},{"Metabolite":"N-Acetylglutamic acid","PubChem ID":"70914","HMDB ID":"HMDB0001138"},{"Metabolite":"2-Hydroxyglutaric acid","PubChem ID":"43","HMDB ID":"HMDB0000606,HMDB0000694"},{"Metabolite":"Carbamoylphosphate","PubChem ID":"278","HMDB ID":"HMDB0001096"},{"Metabolite":"Succinic acid","PubChem ID":"1110","HMDB ID":"HMDB0000254"},{"Metabolite":"Malic acid","PubChem ID":"525","HMDB ID":"HMDB0000156,HMDB0000744"},{"Metabolite":"2-Oxoglutaric acid","PubChem ID":"51","HMDB ID":"HMDB0000208"},{"Metabolite":"Fumaric acid","PubChem ID":"444972","HMDB ID":"HMDB0000134"},{"Metabolite":"Citric acid","PubChem ID":"311","HMDB ID":"HMDB0000094"},{"Metabolite":"cis-Aconitic acid","PubChem ID":"643757","HMDB ID":"HMDB0000072"},{"Metabolite":"Isocitric acid","PubChem ID":"1198","HMDB ID":"HMDB0000193"}] } }