{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002759","ANALYSIS_ID":"AN004479","VERSION":"1","CREATED_ON":"June 26, 2023, 9:34 am"},

"PROJECT":{"PROJECT_TITLE":"SD Rat Metabolomics in Response to Salt","PROJECT_TYPE":"Untargeted Four-Mode Metabolomics","PROJECT_SUMMARY":"This study analyzed the effects of a high salt (HS; 4.0% NaCl) diet upon the kidney, arterial plasma, and renal venous plasma metabolomic profiles of normal Sprague-Dawley rats.","INSTITUTE":"Medical College of Wisconsin","DEPARTMENT":"Physiology","LABORATORY":"Dr. Allen W. Cowley","LAST_NAME":"Cowley","FIRST_NAME":"Allen","ADDRESS":"8701 W. Watertown Plank Rd, Milwaukee, WI 53226","EMAIL":"cowley@mcw.edu","PHONE":"414-955-8277","FUNDING_SOURCE":"NHLBI","CONTRIBUTORS":"Satoshi Shimada, Brian R. Hoffmann, Chun Yang, Theresa Kurth, Andrew S. Greene, Mingyu Liang, Ranjan K. Dash, Allen W. Cowley Jr"},

"STUDY":{"STUDY_TITLE":"Metabolic responses of normal rat kidneys to a high salt intake (Kidney cortex)","STUDY_TYPE":"Time-course metabolomics experiment","STUDY_SUMMARY":"In this study, novel methods were developed which allowed continuous (24/7) measurement of arterial blood pressure and renal blood flow in freely moving rats and the intermittent collection of arterial and renal venous blood to estimate kidney metabolic fluxes of O2 and metabolites. Specifically, the study determined the effects of a high salt (HS; 4.0% NaCl) diet upon whole kidney O2 consumption and arterial and renal venous plasma metabolomic profiles of normal Sprague-Dawley rats. A separate group of rats was studied to determine changes in the cortex and outer medulla tissue metabolomic profiles before and following the switch from a 0.4% to 4.0% NaCl diet.","INSTITUTE":"Medical College of Wisconsin","DEPARTMENT":"Physiology","LABORATORY":"Dr. Allen W. Cowley","LAST_NAME":"Cowley","FIRST_NAME":"Allen","ADDRESS":"8701 W. Watertown Plank Rd, Milwaukee, WI 53226","EMAIL":"cowley@mcw.edu","PHONE":"4149558277"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Rattus norvegicus","TAXONOMY_ID":"10116"},
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},
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},
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"Sample ID":"20220113_Cowley3_Blank_HILICpos_2",
"Factors":{"Treatment":"n/a","Source":"n/a"},
"Additional sample data":{"Instrument Mode":"HILICpositive","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220113_Cowley3_Blank_HILICpos_2","Sample Type":"Blank","Sample Group":"n/a"}
},
{
"Subject ID":"-",
"Sample ID":"20220113_Cowley3_Cortex_QC-Pool_HILICpos_10uL_2",
"Factors":{"Treatment":"n/a","Source":"Cortex Pool"},
"Additional sample data":{"Instrument Mode":"HILICpositive","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220113_Cowley3_Cortex_QC-Pool_HILICpos_10uL_2","Sample Type":"Quality Control","Sample Group":"n/a"}
},
{
"Subject ID":"-",
"Sample ID":"20220113_Cowley3_Blank_HILICpos_3",
"Factors":{"Treatment":"n/a","Source":"n/a"},
"Additional sample data":{"Instrument Mode":"HILICpositive","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220113_Cowley3_Blank_HILICpos_3","Sample Type":"Blank","Sample Group":"n/a"}
},
{
"Subject ID":"-",
"Sample ID":"20220113_Cowley3_Cortex_QC-Pool_HILICpos_10uL_3",
"Factors":{"Treatment":"n/a","Source":"Cortex Pool"},
"Additional sample data":{"Instrument Mode":"HILICpositive","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220113_Cowley3_Cortex_QC-Pool_HILICpos_10uL_3","Sample Type":"Quality Control","Sample Group":"n/a"}
},
{
"Subject ID":"-",
"Sample ID":"20220113_Cowley3_Blank_HILICpos_4",
"Factors":{"Treatment":"n/a","Source":"n/a"},
"Additional sample data":{"Instrument Mode":"HILICpositive","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220113_Cowley3_Blank_HILICpos_4","Sample Type":"Blank","Sample Group":"n/a"}
},
{
"Subject ID":"-",
"Sample ID":"20220113_Cowley3_Cortex_QC-Pool_HILICpos_10uL_4",
"Factors":{"Treatment":"n/a","Source":"Cortex Pool"},
"Additional sample data":{"Instrument Mode":"HILICpositive","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220113_Cowley3_Cortex_QC-Pool_HILICpos_10uL_4","Sample Type":"Quality Control","Sample Group":"n/a"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_2_23-402_HILICneg",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR2","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_2_23-402_HILICneg","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_2_23-402_HILICneg_rep",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR2","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_2_23-402_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_3_25-405_HILICneg",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR3","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_3_25-405_HILICneg","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_3_25-405_HILICneg_rep",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR3","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_3_25-405_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_5_29-413_HILICneg",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR5","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_5_29-413_HILICneg","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_5_29-413_HILICneg_rep",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR5","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_5_29-413_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_10_27-406_HILICneg",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR4","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_10_27-406_HILICneg","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_10_27-406_HILICneg_rep",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR4","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_10_27-406_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_13_21-391_HILICneg",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR1","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_13_21-391_HILICneg","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_13_21-391_HILICneg_rep",
"Factors":{"Treatment":"HS14","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR1","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_13_21-391_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS14, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_7_17-434_HILICneg",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR4","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_7_17-434_HILICneg","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_7_17-434_HILICneg_rep",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR4","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_7_17-434_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_8_19-463_HILICneg",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR5","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_8_19-463_HILICneg","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_8_19-463_HILICneg_rep",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR5","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_8_19-463_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_9_15-429_HILICneg",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR3","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_9_15-429_HILICneg","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_9_15-429_HILICneg_rep",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR3","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_9_15-429_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_12_11-417_HILICneg",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR1","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_12_11-417_HILICneg","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_12_11-417_HILICneg_rep",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR1","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_12_11-417_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_15_13-421_HILICneg",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR2","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_15_13-421_HILICneg","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_15_13-421_HILICneg_rep",
"Factors":{"Treatment":"HS21","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR2","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_15_13-421_HILICneg_rep","Sample Type":"Sample","Sample Group":"HS21, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_1_1-466_HILICneg",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR1","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_1_1-466_HILICneg","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_1_1-466_HILICneg_rep",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR1","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_1_1-466_HILICneg_rep","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_4_3-467_HILICneg",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR2","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_4_3-467_HILICneg","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_4_3-467_HILICneg_rep",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR2","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_4_3-467_HILICneg_rep","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_6_5-468_HILICneg",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR3","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_6_5-468_HILICneg","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_6_5-468_HILICneg_rep",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR3","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_6_5-468_HILICneg_rep","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_11_9-470_HILICneg",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR5","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_11_9-470_HILICneg","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_11_9-470_HILICneg_rep",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR5","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_11_9-470_HILICneg_rep","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_14_7-469_HILICneg",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR4","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_14_7-469_HILICneg","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_14_7-469_HILICneg_rep",
"Factors":{"Treatment":"LS","Source":"Cortex"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"BR4","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_14_7-469_HILICneg_rep","Sample Type":"Sample","Sample Group":"LS, Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_QC-Pool_HILICneg_10uL_1",
"Factors":{"Treatment":"n/a","Source":"Cortex Pool"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_QC-Pool_HILICneg_10uL_1","Sample Type":"Quality Control","Sample Group":"n/a, Cortex Pool"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_QC-Pool_HILICneg_10uL_2",
"Factors":{"Treatment":"n/a","Source":"Cortex Pool"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_QC-Pool_HILICneg_10uL_2","Sample Type":"Quality Control","Sample Group":"n/a, Cortex Pool"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_QC-Pool_HILICneg_10uL_3",
"Factors":{"Treatment":"n/a","Source":"Cortex Pool"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_QC-Pool_HILICneg_10uL_3","Sample Type":"Quality Control","Sample Group":"n/a, Cortex Pool"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Cortex_QC-Pool_HILICneg_10uL_4",
"Factors":{"Treatment":"n/a","Source":"Cortex Pool"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Cortex_QC-Pool_HILICneg_10uL_4","Sample Type":"Quality Control","Sample Group":"n/a, Cortex Pool"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Blank_HILICneg_1",
"Factors":{"Treatment":"n/a","Source":"n/a"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Blank_HILICneg_1","Sample Type":"Blank","Sample Group":"n/a, n/a"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Blank_HILICneg_2",
"Factors":{"Treatment":"n/a","Source":"n/a"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Blank_HILICneg_2","Sample Type":"Blank","Sample Group":"n/a, n/a"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Blank_HILICneg_3",
"Factors":{"Treatment":"n/a","Source":"n/a"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Blank_HILICneg_3","Sample Type":"Blank","Sample Group":"n/a, n/a"}
},
{
"Subject ID":"-",
"Sample ID":"20220118_Cowley3_Blank_HILICneg_4",
"Factors":{"Treatment":"n/a","Source":"n/a"},
"Additional sample data":{"Instrument Mode":"HILICnegative","Biological Replicate":"n/a","CF":"Cortex","RAW_FILE_NAME":"20220118_Cowley3_Blank_HILICneg_4","Sample Type":"Blank","Sample Group":"n/a, n/a"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Plasma was collected through an arterial and renal venous catheter throughout the study (200 µL of arterial and renal venous blood were sampled at the day 7, 14, and 21). Overnight urine (18 hours) from the day before the blood draw was collected on ice. The kidneys were collected either at 14 days of HS (HS14) or 21 days of HS (HS21). The kidneys of only LS fed SD rats were also collected for comparison. The collected kidneys (n=5 for each group for metabolomics) were dissected to cortex and outer medulla and snap frozen with liquid nitrogen. Plasma, urine and tissue were stored in -80°C until further analysis.","SAMPLE_TYPE":"Kidney cortex","STORAGE_CONDITIONS":"-80?"},

"TREATMENT":{"TREATMENT_SUMMARY":"Rats (n=7, 10-11 weeks of age) were performed renal blood flow (RBF) probe implantation and femoral arterial catheterization5. Briefly, rats were anesthetized with isoflurane and arterial catheter was inserted. Following an abdominal incision, RBF probe was implanted on left renal artery and the cable was exposed at nape of the neck via the subcutaneous route. In addition to the RBF probe implantation, renal venous catheter was inserted through the femoral vein and placed in the left renal vein and secured to the luminal wall with 10-0 nylon. RBF and BP via arterial line were measured by conscious freely moving rats and recorded on average of every minute for 24 h/day. After 7-10 days of recovery period, 200 µL of arterial and renal venous blood were sampled and that blood was replaced from donor rats before and following 7, 14 and 21 days after the switch in diet from 0.4% (LS) to 4.0% (HS) salt diet (Dyets Inc, Bethlehem, PA). Overnight urine (18 hours) from the day before the blood draw was collected on ice. The kidneys were collected either at 14 days of HS (HS14) or 21 days of HS (HS21). The kidneys of only LS fed SD rats were also collected for comparison. The collected kidneys (n=5 for each group for metabolomics and mRNAseq analysis) were dissected to cortex and outer medulla and snap frozen with liquid nitrogen. Plasma, urine and tissue were stored in -80°C until further analysis."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Plasma/Urine Metabolite Extraction. Metabolites were extracted from 20 µL of plasma and 20 µL of urine from each SD rat in the study according to standard operating procedures in the Mass Spectrometry and Protein Chemistry Service at The Jackson Laboratory34. Metabolites were extracted using 500 µL of an ice cold 2:2:1 methanol:acetonitrile:water (MeOH:ACN:H2O) buffer; the sample was part of the water fraction. Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards. Each sample was then vortexed for 30 seconds on the highest setting, subject to one minute of mixing with the Tissue Lyser II in pre-chilled cassettes, and then sonicated at 30 Hz for 5 minutes of 30 seconds on 30 seconds off in an ice water bath. Samples were then placed in the -20°C freezer overnight (16 hours) for extraction. Following the extraction, samples were centrifuged at 21,000 x g at 4°C and supernatant from each metabolite extract was equally divided into five 2 mL microcentrifuge tubes. Each sample supernatant was divided into five equal volume aliquots, one for each of the four modes and the rest to create equal representation pools of all samples, one for each mode. Each aliquot was then dried down using a vacuum centrifuge for storage at -80°C until further use. Tissue Metabolite Extraction. Metabolites were extracted from 20 mg of kidney cortex and medulla from each SD rat in the study according to standard operating procedures in the Mass Spectrometry and Protein Chemistry Service at The Jackson Laboratory34 as described for the plasma and urine samples with slight modification. Metabolites were extracted using 1000 µL of an ice cold 2:2:1 methanol:acetonitrile:water (MeOH:ACN:H2O) buffer containing internal standards as above per 20 mg of sample to ensure the extraction equivalents were normalized. Each sample had a 5 mm stainless steel bead added, then were pulverized in extraction buffer for two minutes usingTissue Lyser II. Samples were then placed in the -20°C freezer overnight (16 hours) for extraction and the supernatant was collected as with the urine/plasma samples. Each sample supernatant was divided into five equal volume aliquots, one for each of the four modes and the rest to create equal representation pools of all samples, one for each mode. Each aliquot was then dried down using a vacuum centrifuge for storage at -80°C until further use."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"This chromatography method was utilized for all C18 positive polarity runs in this study.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Agilent InfinityLab Poroshell 120 EC-C18 (2.1 x 50 mm; 2.7-Micron)","SOLVENT_A":"100% water, 0.2% acetic acid","SOLVENT_B":"100% acetonitrile, 0.2% acetic acid","FLOW_GRADIENT":"0-1 minutes at 98% A1/2% B1, 1-13 minutes from 98% A1/2% B1 to 10% A1/90% B1, 13-15 minutes at 10% A1/90% B1, 15-16 minutes from 10% A1/90% B1 to 98% A1/2% B1, and was re-equilibrated from 16-25 minutes at 98% A1/2% B1","FLOW_RATE":"0.1 mL/minute","COLUMN_TEMPERATURE":"25C","INTERNAL_STANDARD":"Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"The Jackson Laboratory Mass Spectrometry and Protein Chemistry Lab"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"C18 positive kidney cortex data: The tandem mass spectrometry RAW data files (consisting of MS1 and MS2 spectra collected) were analyzed using Thermo Compound Discoverer (v3.2.0.421). The MS1 and MS2 data was searched against the Thermo mzCloud database, ChemSpider database, Metabolika Pathways, and mzLogic predicted composition in the Compound Discoverer workflow."},

"MS_METABOLITE_DATA":{
"Units":"Area",

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}