{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002824","ANALYSIS_ID":"AN004607","VERSION":"1","CREATED_ON":"08-23-2023"},

"PROJECT":{"PROJECT_TITLE":"Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia","PROJECT_SUMMARY":"We performed metabolomic analysis to measure polar and lipid metabolites in the serum from TgLC and TgL mice with Tamoxifen (TAM) injection. We compared the levels of lipid metabolites in the liver from TgLC and TgL mice with TAM injection. The lipid metabolites levels in the liver from Balb/c mice bearing C26 or C26-LIF KO tumors were also measured. Mice under both fed and fasted conditions were included. To investigate whether fenofibrate can restore hepatic lipid homeostasis, TgLC mice were fed with a regular chow or fenofibrate diet. Lipid metabolites in liver tissues were analyzed after TAM injection. A similar effect of fenofibrate on lipid metabolites was observed in C26 tumor-bearing mice.","INSTITUTE":"Rutgers University","DEPARTMENT":"Department of Radiation Oncology","LAST_NAME":"Hu","FIRST_NAME":"Wenwei","ADDRESS":"195 Little Albany St, New Brunswick, NJ 08901","EMAIL":"wh221@cinj.rutgers.edu","PHONE":"7322356169","DOI":"http://dx.doi.org/10.21228/M8FB1R"},

"STUDY":{"STUDY_TITLE":"Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia","STUDY_SUMMARY":"We performed metabolomic analysis to measure polar and lipid metabolites in the serum from TgLC and TgL mice with Tamoxifen (TAM) injection. Mice under both fed and fasted conditions were included.","INSTITUTE":"Rutgers University","LAST_NAME":"Hu","FIRST_NAME":"Wenwei","ADDRESS":"195 Little Albany St, New Brunswick, NJ 08901","EMAIL":"wh221@cinj.rutgers.edu","PHONE":"7322356169","SUBMIT_DATE":"2023-08-18"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"TgLIF_S_1F",
"Factors":{"Genotype":"TgLC","Condition":"Fasted","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"TgLIF_S_1F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"TgLIF_S_1M",
"Factors":{"Genotype":"TgLC","Condition":"Fasted","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"TgLIF_S_1M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"TgLIF_S_2F",
"Factors":{"Genotype":"TgLC","Condition":"Fasted","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"TgLIF_S_2F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"TgLIF_S_2M",
"Factors":{"Genotype":"TgLC","Condition":"Fasted","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"TgLIF_S_2M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_Tglif-S_1m",
"Factors":{"Genotype":"TgLC","Condition":"Fasted","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_Tglif-S_1m.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_Tglif-S_2f",
"Factors":{"Genotype":"TgLC","Condition":"Fasted","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_Tglif-S_2f.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_Tglif-S_2m",
"Factors":{"Genotype":"TgLC","Condition":"Fasted","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_Tglif-S_2m.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_Tglif-S_3m",
"Factors":{"Genotype":"TgLC","Condition":"Fasted","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_Tglif-S_3m.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"TgLIF_F_1F",
"Factors":{"Genotype":"TgLC","Condition":"Fed","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"TgLIF_F_1F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"TgLIF_F_1M",
"Factors":{"Genotype":"TgLC","Condition":"Fed","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"TgLIF_F_1M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"TgLIF_F_2F",
"Factors":{"Genotype":"TgLC","Condition":"Fed","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"TgLIF_F_2F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"TgLIF_F_2M",
"Factors":{"Genotype":"TgLC","Condition":"Fed","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"TgLIF_F_2M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_Tglif-F_1f",
"Factors":{"Genotype":"TgLC","Condition":"Fed","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_Tglif-F_1f.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_Tglif-F_1m",
"Factors":{"Genotype":"TgLC","Condition":"Fed","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_Tglif-F_1m.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_Tglif-F_2f",
"Factors":{"Genotype":"TgLC","Condition":"Fed","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_Tglif-F_2f.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_Tglif-F_2m",
"Factors":{"Genotype":"TgLC","Condition":"Fed","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_Tglif-F_2m.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_1F",
"Factors":{"Genotype":"TgL","Condition":"Fasted","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"WT_S_1F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_1M",
"Factors":{"Genotype":"TgL","Condition":"Fasted","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"WT_S_1M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_2F",
"Factors":{"Genotype":"TgL","Condition":"Fasted","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"WT_S_2F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_S_2M",
"Factors":{"Genotype":"TgL","Condition":"Fasted","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"WT_S_2M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_WT-S_1f",
"Factors":{"Genotype":"TgL","Condition":"Fasted","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_WT-S_1f.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_WT-S_2f",
"Factors":{"Genotype":"TgL","Condition":"Fasted","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_WT-S_2f.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_WT-S_3f",
"Factors":{"Genotype":"TgL","Condition":"Fasted","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_WT-S_3f.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_WT-S_3m",
"Factors":{"Genotype":"TgL","Condition":"Fasted","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_WT-S_3m.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_F_1F",
"Factors":{"Genotype":"TgL","Condition":"Fed","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"WT_F_1F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_F_1M",
"Factors":{"Genotype":"TgL","Condition":"Fed","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"WT_F_1M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_F_2F",
"Factors":{"Genotype":"TgL","Condition":"Fed","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"WT_F_2F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"WT_F_2M",
"Factors":{"Genotype":"TgL","Condition":"Fed","Metabolites":"lipid"},
"Additional sample data":{"RAW_FILE_NAME":"WT_F_2M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_WT-F_1f",
"Factors":{"Genotype":"TgL","Condition":"Fed","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_WT-F_1f.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_WT-F_1m",
"Factors":{"Genotype":"TgL","Condition":"Fed","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_WT-F_1m.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_WT-F_2f",
"Factors":{"Genotype":"TgL","Condition":"Fed","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_WT-F_2f.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Serum_WT-F_2m",
"Factors":{"Genotype":"TgL","Condition":"Fed","Metabolites":"polar"},
"Additional sample data":{"RAW_FILE_NAME":"Serum_WT-F_2m.mzXML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Whole blood was collected and centrifuged at 9000 rpm for 10 minutes. The supernatant is serum. Serum was then stored at -80 °C.","SAMPLE_TYPE":"Blood (serum)"},

"TREATMENT":{"TREATMENT_SUMMARY":"TgL and TgLC mice were injected with Tamoxifen under the fed and fasted conditions."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"For lipidomic metabolites extraction, 10 μL serum were mixed with 75 μL methanol and 250 μL Methyl tert-butyl ether (MTBE). After shaking for 6 minutes at 4 °C, 62 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further analysis. Polar metabolites were extracted from serum using the extraction buffer containing methanol: acetonitrile: H2O (40:40:20)."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Lipidomics method","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)","COLUMN_TEMPERATURE":"55C","FLOW_GRADIENT":"0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B","FLOW_RATE":"0.2mL/min","SOLVENT_A":"90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate","SOLVENT_B":"75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate","CHROMATOGRAPHY_TYPE":"Reversed phase"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ACQUISITION_PARAMETERS_FILE":"LCMS_Neg_30min_55C_LongElution"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Plus Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","MS_COMMENTS":"Negative Ionization mode","ION_MODE":"NEGATIVE","CAPILLARY_TEMPERATURE":"300","ION_SOURCE_TEMPERATURE":"360","SPRAY_VOLTAGE":"2700","AUTOMATIC_GAIN_CONTROL":"3000000"},

"MS_METABOLITE_DATA":{
"Units":"Ion counts",

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