{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003032","ANALYSIS_ID":"AN004969","VERSION":"1","CREATED_ON":"January 2, 2024, 3:38 pm"},

"PROJECT":{"PROJECT_TITLE":"Effects of Preanalytical Sample Collection and Handling on Comprehensive Metabolite Measurements in Human Urine Biospecimens","PROJECT_TYPE":"LCMS","PROJECT_SUMMARY":"This project evaluates the impact of sample handling conditions on urine metabolome profiles relative to a gold standard condition. The sample handling conditions included a combination of three variables, use of preservative (None, Borate, or Chlorhexidine), refrigeration storage time (0h or 24h), or freeze-thaws (either 1x or 3x freeze-thaws, thawed on ice, in a refrigerator, or at room temperature). Samples were taken from 13 participants, and then aliquoted and subjected to the various conditions. Comparisons between treatment effects were taken between pairs of samples within each participant’s sample set, a within participant paired design.","INSTITUTE":"National Center for Advancing Tranlastional Sciences","DEPARTMENT":"Division of Pre-clinical Innovation","LABORATORY":"Informatics Core","LAST_NAME":"Braisted","FIRST_NAME":"John","ADDRESS":"9800 Medical Center Drive","EMAIL":"john.braisted@nih.gov","PHONE":"3014028798","FUNDING_SOURCE":"ZIC TR000410-05, NCATS/NIH"},

"STUDY":{"STUDY_TITLE":"Effects of Preanalytical Sample Collection and Handling on Comprehensive Metabolite Measurements in Human Urine Biospecimens","STUDY_TYPE":"LCMS Investigation of Urine Metabolite Stability","STUDY_SUMMARY":"This study evaluates the impact of sample handling conditions on urine metabolome profiles relative to a gold standard condition. The sample handling conditions included a combination of three variables, use of preservative (None, Borate, or Chlorhexidine), refrigeration storage time (0h or 24h), or freeze-thaws (either 1x or 3x freeze-thaws, thawed on ice, in a refrigerator, or at room temperature). Samples were taken from 13 participants, and then aliquoted and subjected to the various conditions. Comparisons between treatment effects were taken between pairs of samples within each participant’s sample set, a within participant paired design.","INSTITUTE":"National Center for Advancing Tranlastional Sciences","DEPARTMENT":"Division of Pre-clinical Innovation","LABORATORY":"Informatics Core","LAST_NAME":"Braisted","FIRST_NAME":"John","ADDRESS":"9800 Medical Center Drive","EMAIL":"john.braisted@nih.gov","PHONE":"3014028798","TOTAL_SUBJECTS":"13","NUM_MALES":"6","NUM_FEMALES":"7"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"23-64","WEIGHT_OR_WEIGHT_RANGE":"54.4-90.7 kg","HEIGHT_OR_HEIGHT_RANGE":"158-184 cm","GENDER":"Male and female","HUMAN_SMOKING_STATUS":"12 - Non-smokers, 1 - Smoker"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"1",
"Sample ID":"5601_U1",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"255","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U2",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"254","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U3",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"504","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U4",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"252","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U5",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"257","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U6",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"253","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U7",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"246","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U8",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"254","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U9",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"254","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U10",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"259","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U11",
"Factors":{"preservative":"none","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"253","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"305","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U13",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"307","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U14",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"306","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U15",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"309","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U16",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"305","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U17",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"299","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U18",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"307","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U19",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"310","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U20",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"306","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U21",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"309","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U22",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"384","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U23",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"382","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"499","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U25",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"383","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U26",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"384","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U27",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"383","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U28",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"375","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U29",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"381","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U30",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"388","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U31",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"382","sex":"F","age":"60"}
},
{
"Subject ID":"1",
"Sample ID":"5601_U32",
"Factors":{"preservative":"Borate","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"390","sex":"F","age":"60"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U1",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"518","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U2",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"517","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U3",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"498","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U4",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"519","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U5",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"518","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U6",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"518","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U7",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"508","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U8",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"517","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U9",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"519","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U10",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"519","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U11",
"Factors":{"preservative":"none","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"518","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"568","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U13",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"569","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U14",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"568","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U15",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"573","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U16",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"576","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U17",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"563","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U18",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"569","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U19",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"573","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U20",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"573","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U21",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"575","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U22",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"644","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U23",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"655","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"502","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U25",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"651","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U26",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"638","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U27",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"641","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U28",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"625","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U29",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"633","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U30",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"637","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U31",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"652","sex":"F","age":"23"}
},
{
"Subject ID":"2",
"Sample ID":"5602_U32",
"Factors":{"preservative":"Borate","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"642","sex":"F","age":"23"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U1",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"351","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U2",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"352","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U3",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"500","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U4",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"358","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U5",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"355","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U6",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"359","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U7",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"342","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U8",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"357","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U9",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"355","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U10",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"358","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U11",
"Factors":{"preservative":"none","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"357","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"407","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U13",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"409","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U14",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"405","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U15",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"407","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U16",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"410","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U17",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"395","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U18",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"407","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U19",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"408","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U20",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"408","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U21",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"408","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U22",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"481","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U23",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"480","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"501","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U25",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"480","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U26",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"476","sex":"M","age":"56"}
},
{
"Subject ID":"3",
"Sample ID":"5603_U27",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 RT"},
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{
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{
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},
{
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{
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},
{
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},
{
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},
{
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},
{
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},
{
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},
{
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},
{
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},
{
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"Sample ID":"5604_U7",
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},
{
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},
{
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"Sample ID":"5604_U9",
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},
{
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"Sample ID":"5604_U10",
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},
{
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"Sample ID":"5604_U11",
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},
{
"Subject ID":"4",
"Sample ID":"5604_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5604_U13",
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},
{
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"Sample ID":"5604_U14",
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},
{
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"Sample ID":"5604_U15",
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},
{
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"Sample ID":"5604_U16",
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},
{
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"Sample ID":"5604_U17",
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},
{
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},
{
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"Sample ID":"5604_U19",
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},
{
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"Sample ID":"5604_U20",
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},
{
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"Sample ID":"5604_U21",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"24","thaw":"none"},
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},
{
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"Sample ID":"5604_U22",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
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},
{
"Subject ID":"4",
"Sample ID":"5604_U23",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
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},
{
"Subject ID":"4",
"Sample ID":"5604_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
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},
{
"Subject ID":"4",
"Sample ID":"5604_U25",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 ice"},
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},
{
"Subject ID":"4",
"Sample ID":"5604_U26",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 fridge"},
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},
{
"Subject ID":"4",
"Sample ID":"5604_U27",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 RT"},
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},
{
"Subject ID":"4",
"Sample ID":"5604_U28",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 ice"},
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},
{
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"Sample ID":"5604_U29",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
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},
{
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"Sample ID":"5604_U30",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
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},
{
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"Sample ID":"5604_U31",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 RT"},
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},
{
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"Sample ID":"5604_U32",
"Factors":{"preservative":"Borate","fridge_store_time":"24","thaw":"none"},
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},
{
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"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5605_U2",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5605_U3",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5605_U4",
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},
{
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"Sample ID":"5605_U5",
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},
{
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"Sample ID":"5605_U6",
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},
{
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"Sample ID":"5605_U7",
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},
{
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"Sample ID":"5605_U8",
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},
{
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"Sample ID":"5605_U9",
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},
{
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"Sample ID":"5605_U10",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 RT"},
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},
{
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"Sample ID":"5605_U11",
"Factors":{"preservative":"none","fridge_store_time":"24","thaw":"none"},
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},
{
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"Sample ID":"5605_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5605_U13",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
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},
{
"Subject ID":"5",
"Sample ID":"5605_U14",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 ice"},
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},
{
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"Sample ID":"5605_U15",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 fridge"},
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},
{
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"Sample ID":"5605_U16",
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},
{
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"Sample ID":"5605_U17",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 ice"},
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},
{
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"Sample ID":"5605_U18",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
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},
{
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"Sample ID":"5605_U19",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
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},
{
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"Sample ID":"5605_U20",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"476","sex":"M","age":"58"}
},
{
"Subject ID":"5",
"Sample ID":"5605_U21",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"24","thaw":"none"},
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},
{
"Subject ID":"5",
"Sample ID":"5605_U22",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"564","sex":"M","age":"58"}
},
{
"Subject ID":"5",
"Sample ID":"5605_U23",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"564","sex":"M","age":"58"}
},
{
"Subject ID":"5",
"Sample ID":"5605_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5605_U25",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"561","sex":"M","age":"58"}
},
{
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"Sample ID":"5605_U26",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"566","sex":"M","age":"58"}
},
{
"Subject ID":"5",
"Sample ID":"5605_U27",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 RT"},
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},
{
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"Sample ID":"5605_U28",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 ice"},
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},
{
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"Sample ID":"5605_U29",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
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},
{
"Subject ID":"5",
"Sample ID":"5605_U30",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"560","sex":"M","age":"58"}
},
{
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"Sample ID":"5605_U31",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 RT"},
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},
{
"Subject ID":"5",
"Sample ID":"5605_U32",
"Factors":{"preservative":"Borate","fridge_store_time":"24","thaw":"none"},
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},
{
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"Sample ID":"5606_U1",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
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},
{
"Subject ID":"6",
"Sample ID":"5606_U2",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
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},
{
"Subject ID":"6",
"Sample ID":"5606_U3",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5606_U4",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1138","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U5",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1145","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U6",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1133","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U7",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1125","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U8",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1108","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U9",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1135","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U10",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 RT"},
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},
{
"Subject ID":"6",
"Sample ID":"5606_U11",
"Factors":{"preservative":"none","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1134","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1185","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U13",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1184","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U14",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1188","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U15",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1199","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U16",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1192","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U17",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1156","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U18",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1191","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U19",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1191","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U20",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1183","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U21",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1200","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U22",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1250","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U23",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1252","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"504","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U25",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1264","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U26",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1264","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U27",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1257","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U28",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1219","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U29",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"1249","sex":"F","age":"26"}
},
{
"Subject ID":"6",
"Sample ID":"5606_U30",
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{
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{
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{
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{
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{
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{
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{
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{
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{
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{
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{
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{
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{
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{
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{
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"Sample ID":"5608_U24",
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"Sample ID":"5608_U25",
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"Sample ID":"5608_U26",
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"Sample ID":"5609_U2",
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"Sample ID":"5609_U4",
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"Sample ID":"5609_U5",
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"Sample ID":"5609_U6",
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"Sample ID":"5609_U7",
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"Sample ID":"5609_U8",
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"Sample ID":"5609_U9",
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{
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"Sample ID":"5609_U10",
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},
{
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"Sample ID":"5609_U11",
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},
{
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"Sample ID":"5609_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
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{
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"Sample ID":"5609_U13",
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{
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"Sample ID":"5609_U14",
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"Sample ID":"5609_U15",
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"Sample ID":"5609_U16",
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"Sample ID":"5609_U17",
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{
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"Sample ID":"5609_U18",
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"Sample ID":"5609_U19",
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{
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"Sample ID":"5609_U20",
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},
{
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"Sample ID":"5609_U21",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"24","thaw":"none"},
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{
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"Sample ID":"5609_U22",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
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{
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"Sample ID":"5609_U23",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
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{
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"Sample ID":"5609_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
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{
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"Sample ID":"5609_U25",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 ice"},
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{
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"Sample ID":"5609_U26",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 fridge"},
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{
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"Sample ID":"5609_U27",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 RT"},
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"Sample ID":"5609_U28",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 ice"},
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{
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"Sample ID":"5609_U29",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
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{
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"Sample ID":"5609_U30",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
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{
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"Sample ID":"5609_U31",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 RT"},
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{
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"Sample ID":"5609_U32",
"Factors":{"preservative":"Borate","fridge_store_time":"24","thaw":"none"},
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{
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"Sample ID":"5610_U1",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
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{
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"Sample ID":"5610_U2",
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},
{
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},
{
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},
{
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},
{
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},
{
"Subject ID":"10",
"Sample ID":"5610_U7",
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},
{
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"Sample ID":"5610_U8",
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},
{
"Subject ID":"10",
"Sample ID":"5610_U9",
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"Additional sample data":{"sample_class":"sample","Osmolality":"192","sex":"F","age":"51"}
},
{
"Subject ID":"10",
"Sample ID":"5610_U10",
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},
{
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"Sample ID":"5610_U11",
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},
{
"Subject ID":"10",
"Sample ID":"5610_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5610_U13",
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},
{
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"Sample ID":"5610_U14",
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},
{
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"Sample ID":"5610_U15",
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},
{
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"Sample ID":"5610_U16",
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},
{
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"Sample ID":"5610_U17",
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},
{
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"Sample ID":"5610_U18",
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},
{
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"Sample ID":"5610_U19",
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},
{
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"Sample ID":"5610_U20",
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},
{
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"Sample ID":"5610_U21",
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},
{
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"Sample ID":"5610_U22",
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},
{
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},
{
"Subject ID":"10",
"Sample ID":"5610_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5610_U25",
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},
{
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"Sample ID":"5610_U26",
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},
{
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"Sample ID":"5610_U27",
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},
{
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"Sample ID":"5610_U28",
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},
{
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"Sample ID":"5610_U29",
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},
{
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"Sample ID":"5610_U30",
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},
{
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"Sample ID":"5610_U31",
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},
{
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"Sample ID":"5610_U32",
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},
{
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},
{
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"Sample ID":"5611_U2",
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},
{
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"Sample ID":"5611_U3",
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},
{
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"Sample ID":"5611_U4",
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},
{
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"Sample ID":"5611_U5",
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},
{
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"Sample ID":"5611_U6",
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},
{
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"Sample ID":"5611_U7",
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},
{
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"Sample ID":"5611_U8",
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},
{
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"Sample ID":"5611_U9",
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},
{
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"Sample ID":"5611_U10",
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},
{
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"Sample ID":"5611_U11",
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},
{
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"Sample ID":"5611_U12",
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},
{
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"Sample ID":"5611_U13",
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},
{
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"Sample ID":"5611_U14",
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},
{
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"Sample ID":"5611_U15",
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},
{
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"Sample ID":"5611_U16",
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},
{
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"Sample ID":"5611_U17",
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},
{
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"Sample ID":"5611_U18",
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},
{
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"Sample ID":"5611_U19",
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},
{
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"Sample ID":"5611_U20",
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},
{
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"Sample ID":"5611_U21",
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},
{
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"Sample ID":"5611_U22",
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},
{
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"Sample ID":"5611_U23",
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},
{
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"Sample ID":"5611_U24",
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},
{
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"Sample ID":"5611_U25",
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},
{
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"Sample ID":"5611_U26",
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},
{
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"Sample ID":"5611_U27",
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},
{
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"Sample ID":"5611_U28",
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},
{
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"Sample ID":"5611_U29",
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},
{
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"Sample ID":"5611_U30",
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},
{
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"Sample ID":"5611_U31",
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},
{
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"Sample ID":"5611_U32",
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},
{
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"Sample ID":"5612_U1",
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},
{
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"Sample ID":"5612_U2",
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},
{
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"Sample ID":"5612_U3",
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},
{
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"Sample ID":"5612_U4",
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},
{
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"Sample ID":"5612_U5",
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},
{
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"Sample ID":"5612_U6",
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},
{
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"Sample ID":"5612_U7",
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},
{
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"Sample ID":"5612_U8",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
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},
{
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"Sample ID":"5612_U9",
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"Additional sample data":{"sample_class":"sample","Osmolality":"631","sex":"M","age":"25"}
},
{
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"Sample ID":"5612_U10",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 RT"},
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},
{
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"Sample ID":"5612_U11",
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},
{
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"Sample ID":"5612_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
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},
{
"Subject ID":"12",
"Sample ID":"5612_U13",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
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},
{
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"Sample ID":"5612_U14",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 ice"},
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},
{
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"Sample ID":"5612_U15",
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},
{
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"Sample ID":"5612_U16",
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},
{
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"Sample ID":"5612_U17",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 ice"},
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},
{
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"Sample ID":"5612_U18",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
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},
{
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"Sample ID":"5612_U19",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
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},
{
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"Sample ID":"5612_U20",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 RT"},
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},
{
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"Sample ID":"5612_U21",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"24","thaw":"none"},
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},
{
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"Sample ID":"5612_U22",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
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},
{
"Subject ID":"12",
"Sample ID":"5612_U23",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"761","sex":"M","age":"25"}
},
{
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"Sample ID":"5612_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"503","sex":"M","age":"25"}
},
{
"Subject ID":"12",
"Sample ID":"5612_U25",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"756","sex":"M","age":"25"}
},
{
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"Sample ID":"5612_U26",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"753","sex":"M","age":"25"}
},
{
"Subject ID":"12",
"Sample ID":"5612_U27",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 RT"},
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},
{
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"Sample ID":"5612_U28",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"720","sex":"M","age":"25"}
},
{
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"Sample ID":"5612_U29",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
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},
{
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"Sample ID":"5612_U30",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"756","sex":"M","age":"25"}
},
{
"Subject ID":"12",
"Sample ID":"5612_U31",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"761","sex":"M","age":"25"}
},
{
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"Sample ID":"5612_U32",
"Factors":{"preservative":"Borate","fridge_store_time":"24","thaw":"none"},
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},
{
"Subject ID":"13",
"Sample ID":"5613_U1",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"275","sex":"M","age":"24"}
},
{
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"Sample ID":"5613_U2",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"274","sex":"M","age":"24"}
},
{
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"Sample ID":"5613_U3",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"498","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U4",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"272","sex":"M","age":"24"}
},
{
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"Sample ID":"5613_U5",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"274","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U6",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"272","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U7",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"265","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U8",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"271","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U9",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"273","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U10",
"Factors":{"preservative":"none","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"273","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U11",
"Factors":{"preservative":"none","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"275","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U12",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"314","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U13",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"317","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U14",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"316","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U15",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"319","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U16",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"317","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U17",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"311","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U18",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"317","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U19",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"320","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U20",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"318","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U21",
"Factors":{"preservative":"chlorhexidine","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"320","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U22",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"392","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U23",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"390","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U24",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"none"},
"Additional sample data":{"sample_class":"pool","Osmolality":"502","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U25",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"395","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U26",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"394","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U27",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"1 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"395","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U28",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 ice"},
"Additional sample data":{"sample_class":"sample","Osmolality":"407","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U29",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"397","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U30",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 fridge"},
"Additional sample data":{"sample_class":"sample","Osmolality":"386","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U31",
"Factors":{"preservative":"Borate","fridge_store_time":"0","thaw":"4 RT"},
"Additional sample data":{"sample_class":"sample","Osmolality":"389","sex":"M","age":"24"}
},
{
"Subject ID":"13",
"Sample ID":"5613_U32",
"Factors":{"preservative":"Borate","fridge_store_time":"24","thaw":"none"},
"Additional sample data":{"sample_class":"sample","Osmolality":"410","sex":"M","age":"24"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mid-stream spot urine collections were obtained for each participant in the mornings into a sterile urine collection cup with a vacutainer port. Participants were not asked to fast. Aliquots were divided into three separate tubes corresponding to the following preservative treatments: 1) no preservative, 2) borate preservative (BD Vacutainer C&S Urine Tubes, borate 2.63 mg/mL, sodium formate 1.65 mg/mL), 3) chlorhexidine preservative (BD Vacutainer Urinalysis Plus, chlorhexidine 0.4%, sodium propionate 94%, ethyl paraben 5.6%). Tubes were centrifuged at 600xg for 5 min. One mL of urine, with no preservative, from each participant was pooled and mixed to be used as a QC sample, which was run repeatedly with other experimentally treated samples during metabolomics profiling (see section Laboratory assays). Ten aliquots of each of the 3 preservative samples (30 samples total per participant) were transferred into 2mL cryotubes to be subjected to various sample handling conditions, namely refrigeration and free-thaw conditions. Conditions tested included 3 preservative conditions (no preservative, chlorhexidine, and borate), 2 refrigeration conditions (24-hour refrigeration, no refrigeration/snap freezing), and 7 freeze-thaw conditions (no freeze-thaw, thaw on ice once or 4 times, thaw in refrigerator once or four times, and thaw at room temperature once or 4 times). One sample of each preservative treatment was placed in the refrigerator for 24 h before freezing in liquid nitrogen and stored at -80 °C. Remaining samples were flash frozen in liquid nitrogen and stored at -80 °C. They were then subject to various thawing conditions and freeze-thaw cycles. Thawing was performed at either room temperature, in a refrigerator, or on wet ice. Freeze thaws consisted of either a single thaw or 4 consecutive freeze-thaw cycles. Samples undergoing freeze thaws were snap frozen in liquid nitrogen following their thaw. Samples undergoing freeze-thaw cycles had the thaw time standardized based on previous work (previous serum study). Thaws on ice were fixed at 50 min, room temperature thaws were fixed at 10 min, and refrigerator thaws were fixed at 16 h to simulate a 5 PM to 9 AM thaw often used in laboratories.","SAMPLE_TYPE":"Urine","COLLECTION_METHOD":"Spot collection, into sterile urine collection cup","COLLECTION_FREQUENCY":"Single Collection","STORAGE_CONDITIONS":"Described in summary","STORAGE_VIALS":"Final Storage in 2ml cryotubes.","ADDITIVES":"None, Chlorhexidine, or Borate (experimental factors)"},

"TREATMENT":{"TREATMENT_SUMMARY":"Conditions tested included 3 preservative conditions (no preservative, chlorhexidine, and borate), 2 refrigeration conditions (24-hour refrigeration, no refrigeration/snap freezing), and 7 freeze-thaw conditions (no freeze-thaw, thaw on ice once or 4 times, thaw in refrigerator once or four times, and thaw at room temperature once or 4 times). 28 samples per participant were treated to a combination of these conditions to evaluate the impact of these various handling conditions on measured metabolite levels. Note that the time for freeze thaws were fixed at 10 minutes for room temperature thaws, 50 minutes for ice thaws, and 16h for refrigerator thaws, to simulate an overnight refrigerator thaw. The study design table indicates to which conditions that each sample was exposed."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"After separating into separate tubes, the samples were centrifuged at 600xg for 5 minutes and the supernatent separated into cryovials and underwent various treatments. Snap-frozen samples were sent to Metabolon Inc. for sample extraction and LCMS according to Metabolon established protocols.","PROCESSING_STORAGE_CONDITIONS":"Described in summary","EXTRACT_STORAGE":"Described in summary"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Low pH polar (LC/MS Pos early)","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity BEH C18 (100 x 2mm, 1.7um)","SOLVENT_A":"100% water; 0.1% formic acid; 0.05% PFPA, pH ~2.5","SOLVENT_B":"100% methanol; 0.1% formic acid; 0.05% PFPA, pH ~2.5","FLOW_GRADIENT":"Linear gradient from 5% B to 80% B over 3.35 minutes","FLOW_RATE":"0.35 mL/min","COLUMN_TEMPERATURE":"40-50"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"Other","ION_MODE":"POSITIVE","MS_COMMENTS":"Metabolon (LC/MS Pos early)"},

"MS_METABOLITE_DATA":{
"Units":"Peak Area",

"Data":[{"Metabolite":"(N(1) + 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