{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003094","ANALYSIS_ID":"AN005064","VERSION":"1","CREATED_ON":"February 17, 2024, 8:52 pm"},

"PROJECT":{"PROJECT_TITLE":"Probiotic intervention of FMT mice","PROJECT_SUMMARY":"L. plantarum intervention of gestational fecal microbiome transplant germ-free mice","INSTITUTE":"Beijing Sanyuan Foods Co. Ltd","LAST_NAME":"Bin","FIRST_NAME":"Liu","ADDRESS":"8th yingchang street, yinghai distric","EMAIL":"liubin@sanyuan.com.cn","PHONE":"01056306594"},

"STUDY":{"STUDY_TITLE":"probiotic on gestational health of FMT GF mice","STUDY_SUMMARY":"probiotic L.plantarum intervention gestational on FMT GF mice","INSTITUTE":"Beijing Sanyuan Foods Co. Ltd","LAST_NAME":"Liu","FIRST_NAME":"Bin","ADDRESS":"8th yingchang street, yinghai distric","EMAIL":"liubin@sanyuan.com.cn","PHONE":"01056306594"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"81",
"Factors":{"Sample source":"serum","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"81.raw"}
},
{
"Subject ID":"-",
"Sample ID":"107",
"Factors":{"Sample source":"serum","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"107.raw"}
},
{
"Subject ID":"-",
"Sample ID":"120",
"Factors":{"Sample source":"serum","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"120.raw"}
},
{
"Subject ID":"-",
"Sample ID":"122",
"Factors":{"Sample source":"serum","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"122.raw"}
},
{
"Subject ID":"-",
"Sample ID":"180",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"180.raw"}
},
{
"Subject ID":"-",
"Sample ID":"185",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"185.raw"}
},
{
"Subject ID":"-",
"Sample ID":"188",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"188.raw"}
},
{
"Subject ID":"-",
"Sample ID":"907",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"907.raw"}
},
{
"Subject ID":"-",
"Sample ID":"908",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"908.raw"}
},
{
"Subject ID":"-",
"Sample ID":"909",
"Factors":{"Sample source":"serum","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"909.raw"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C environment","SAMPLE_TYPE":"Blood (serum)","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were vortex for 1 min after adding 0.4 mL methanol; then they were centrifuged for 10 min at 12,000 rpm at 4^oC, and the supernatant was dried and resolved using 0.15 mL 2-chloro-l-phenylalanine (4 ppm) solution, and then filtered by 0.22 μm membrane","PROCESSING_STORAGE_CONDITIONS":"4℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"LC","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)","SOLVENT_A":"formic acid in water","SOLVENT_B":"formic acid in acetonitrile","FLOW_GRADIENT":"0~1 min, 2% B2; 1~9 min, 2%~50% B2; 9~12 min, 50%~98% B2; 12~13.5 min, 98% B2; 13.5~14 min, 98%~2% B2; 14~20 min, 2% B2","FLOW_RATE":"0.25 mL/min","COLUMN_TEMPERATURE":"40"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Orbitrap Exploris 480","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"MS1 range was 100-1000 m/z, and the number of data-dependent scans per cycle was 4; MS/MS resolving power was 15000 FWHM, normalized collision energy was 30%, and dynamic exclusion time was automatic. The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment.","MS_RESULTS_FILE":"ST003094_AN005064_Results.txt UNITS:m/z values Has m/z:Yes Has RT:Yes RT units:Minutes"}

}