{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003267","ANALYSIS_ID":"AN005352","PROJECT_ID":"PR002029","VERSION":"1","CREATED_ON":"June 18, 2024, 12:27 pm"},

"PROJECT":{"PROJECT_TITLE":"FDX2-KO induces global down-regulation of iron-sulfur cluster-containing proteins and senescence-like growth arrest or death in ovarian cancer cells","PROJECT_SUMMARY":"Recent studies report molecular mechanisms underlying iron-sulfur cluster (Fe-S) biosynthesis and suggest its importance in health and disease. However, a role for Fe-S biosynthesis in cancer contexts remains unclear. Here we report that FDX2, an Fe-S assembly factor, is indispensable for maintenance of cellular Fe-S-containing proteins (Fe-S protein(s)) and proliferation of ovarian cancer (OVC) cells. CRISPR-screening of all metabolism-related genes in OVC cells identified several Fe-S assembly genes as essential for OVC growth. Using an inducible FDX2-KO OVC line, we found that FDX2 loss promotes either senescence-like growth arrest or cell death, depending on p53 status. Mechanistically, FDX2-loss caused global but differential post transcriptional down-regulation of Fe-S proteins, in turn perturbing respiration, iron-regulation and redox homeostasis, all associated with DNA damage. These results demonstrate significant roles for Fe-S biosynthesis in OVC proliferation and survival and provide information about how the cellular Fe-S-protein network responds to disruptions in Fe-S assembly.","INSTITUTE":"Tohoku University","DEPARTMENT":"Graduate School of Medicine","LABORATORY":"Department of Biochemical Oncology","LAST_NAME":"Tanuma","FIRST_NAME":"Nobu-hiro","ADDRESS":"2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan","EMAIL":"nobuhiro.tanuma.c7@tohoku.ac.jp","PHONE":"+81-22-381-1165"},

"STUDY":{"STUDY_TITLE":"FDX2-KO induces global down-regulation of iron-sulfur cluster-containing proteins and senescence-like growth arrest or death in ovarian cancer cells","STUDY_SUMMARY":"Recent studies report molecular mechanisms underlying iron-sulfur cluster (Fe-S) biosynthesis and suggest its importance in health and disease. However, a role for Fe-S biosynthesis in cancer contexts remains unclear. Here we report that FDX2, an Fe-S assembly factor, is indispensable for maintenance of cellular Fe-S-containing proteins (Fe-S protein(s)) and proliferation of ovarian cancer (OVC) cells. CRISPR-screening of all metabolism-related genes in OVC cells identified several Fe-S assembly genes as essential for OVC growth. Using an inducible FDX2-KO OVC line, we found that FDX2 loss promotes either senescence-like growth arrest or cell death, depending on p53 status. Mechanistically, FDX2-loss caused global but differential post transcriptional down-regulation of Fe-S proteins, in turn perturbing respiration, iron-regulation and redox homeostasis, all associated with DNA damage. These results demonstrate significant roles for Fe-S biosynthesis in OVC proliferation and survival and provide information about how the cellular Fe-S-protein network responds to disruptions in Fe-S assembly.","INSTITUTE":"Tohoku University","DEPARTMENT":"Graduate School of Medicine","LABORATORY":"Department of Biochemical Oncology","LAST_NAME":"Tanuma","FIRST_NAME":"Nobu-hiro","ADDRESS":"2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan","EMAIL":"nobuhiro.tanuma.c7@tohoku.ac.jp","PHONE":"+81-22-381-1165"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"1",
"Factors":{"Sample source":"Ovarian cancer cells","DOX":"On"},
"Additional sample data":{"Amount of protein (ug)":"399.3","Day":"Day 5","RAW_FILE_NAME":"1_d1"}
},
{
"Subject ID":"-",
"Sample ID":"2",
"Factors":{"Sample source":"Ovarian cancer cells","DOX":"On"},
"Additional sample data":{"Amount of protein (ug)":"435.6","Day":"Day 5","RAW_FILE_NAME":"2_d1"}
},
{
"Subject ID":"-",
"Sample ID":"3",
"Factors":{"Sample source":"Ovarian cancer cells","DOX":"On"},
"Additional sample data":{"Amount of protein (ug)":"459.8","Day":"Day 5","RAW_FILE_NAME":"3_d1"}
},
{
"Subject ID":"-",
"Sample ID":"4",
"Factors":{"Sample source":"Ovarian cancer cells","DOX":"On"},
"Additional sample data":{"Amount of protein (ug)":"435.6","Day":"Day 5","RAW_FILE_NAME":"4_d1"}
},
{
"Subject ID":"-",
"Sample ID":"5",
"Factors":{"Sample source":"Ovarian cancer cells","DOX":"OFF"},
"Additional sample data":{"Amount of protein (ug)":"617.1","Day":"Day 5","RAW_FILE_NAME":"5_d1"}
},
{
"Subject ID":"-",
"Sample ID":"6",
"Factors":{"Sample source":"Ovarian cancer cells","DOX":"OFF"},
"Additional sample data":{"Amount of protein (ug)":"701.8","Day":"Day 5","RAW_FILE_NAME":"6_d1"}
},
{
"Subject ID":"-",
"Sample ID":"7",
"Factors":{"Sample source":"Ovarian cancer cells","DOX":"OFF"},
"Additional sample data":{"Amount of protein (ug)":"726","Day":"Day 5","RAW_FILE_NAME":"7_d1"}
},
{
"Subject ID":"-",
"Sample ID":"8",
"Factors":{"Sample source":"Ovarian cancer cells","DOX":"OFF"},
"Additional sample data":{"Amount of protein (ug)":"713.9","Day":"Day 5","RAW_FILE_NAME":"8_d1"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Cells were maintained in DMEM medium (high glucose) in the presence of doxycycline (DOX). For metabolome analysis, cells were seeded to new dishes at day 0 and cultured in the presence or absence of Dox for 4 days. At day 4, cells were replenished with fresh medium (with or without Dox) and cultured for additional one day. At day 5, cells were washed with PBS twice and detached from dishes using cell scraper into PBS. The detached cells were collected by centrifugation, snap-frozen by liquid nitrogen, and stored at -80 oC until metabolite extraction.","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"FDX2-iKO JHOC5 cells were cultured 5 days with or without doxycycline (30 ng/ml) and collected."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"The cell extracts were centrifugally concentrated for 2.5 hours using a LABCONCO centrifugal concentrator with cooling function (LABCONCO corporation)."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Column information: Fused silica capillary tubing 50 um i.d. x 200 cm (cut to 100 cm for analysis), Manufacturer: Molex, Material number: 106815-0017, Product description: TSP050375, Lot: CHCC04A","CHROMATOGRAPHY_TYPE":"CE","INSTRUMENT_NAME":"Agilent 7100 CE","COLUMN_NAME":"Molex Fused silica capillary tubing (100cm x 50um)","SOLVENT_A":"(Not applicable)","SOLVENT_B":"(Not applicable)","FLOW_GRADIENT":"(Not applicable)","FLOW_RATE":"10 uL/min","COLUMN_TEMPERATURE":"20","INTERNAL_STANDARD":"Methionine sulfone","SAMPLE_INJECTION":"Pressure injection 50 mbar, 5 sec (approximately 5 nL)","CAPILLARY_VOLTAGE":"Positive, 30 kV","RUNNING_BUFFER":"1 M formic acid","SHEATH_LIQUID":"50% (v/v) methanol-water containing 0.01 uM hexakis(2,2-difluoroethoxy)phosphazene"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Institute for Advanced Biosciences, Keio University","SOFTWARE_VERSION":"MassHunter workstation software version B.08.00","ACQUISITION_DATE":"April 3, 2024"},

"MS":{"INSTRUMENT_NAME":"Agilent 6230 TOF","INSTRUMENT_TYPE":"TOF-MS","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"ESI-TOFMS was performed in positive ion mode. Automatic recalibration of each acquired spectrum was achieved using the masses of the reference standards (13C isotopic ion of a protonated methanol dimer, m/z 66.0631) and (protonated ion of hexakis(2,2-difluoroethoxy)phosphazene, m/z 622.0290). For system control and data acquisition we used the MassHunter workstation software for Agilent CE-TOFMS. CE-TOFMS raw data were analyzed using our proprietary software MasterHands (ver, 2.20.0.3).","CAPILLARY_VOLTAGE":"4,000 V","DRY_GAS_FLOW":"Nitrogen, 10 L/min","DRY_GAS_TEMP":"300℃","FRAGMENT_VOLTAGE":"75 V","IONIZATION":"ESI","OCTPOLE_VOLTAGE":"500 V"},

"MS_METABOLITE_DATA":{
"Units":"μmol/g-protein",

"Data":[{"Metabolite":"Urea","1":"9.439345485","2":"5.103312269","3":"3.770002738","4":"19.73469614","5":"6.200814491","6":"24.99986517","7":"7.522061847","8":"6.352478219"},{"Metabolite":"Gly","1":"5.279520363","2":"4.457316316","3":"4.373997686","4":"3.751143346","5":"3.967636312","6":"3.029093612","7":"2.986937304","8":"3.658375196"},{"Metabolite":"Trimethylamine N-oxide","1":"0.04630675","2":"0.051348201","3":"0.051533021","4":"0.036742632","5":"0.037047858","6":"0.036641094","7":"0.025431645","8":"0.025238686"},{"Metabolite":"Putrescine(1,4-Butanediamine)","1":"0.074124479","2":"0.099972708","3":"0.115265103","4":"0.098234122","5":"0.095762846","6":"0.096700807","7":"0.085411259","8":"0.111790084"},{"Metabolite":"beta-Ala","1":"0.761777191","2":"1.079534715","3":"1.053635097","4":"1.007138051","5":"0.573981285","6":"0.476928753","7":"0.339008072","8":"0.661983467"},{"Metabolite":"Ala","1":"2.271786881","2":"1.790227914","3":"1.713357628","4":"1.539115522","5":"2.016714772","6":"1.748454383","7":"1.816165041","8":"2.030514267"},{"Metabolite":"Cyclohexylamine","1":"","2":"","3":"","4":"0.042686034","5":"","6":"","7":"0.030050273","8":""},{"Metabolite":"GABA","1":"0.140507251","2":"0.093435713","3":"0.054085338","4":"0.047139882","5":"0.072781886","6":"0.033874522","7":"0.034830627","8":"0.027883287"},{"Metabolite":"2AB","1":"0.117855179","2":"0.128847788","3":"0.054864895","4":"0.086160123","5":"0.085082031","6":"0.066761272","7":"0.071036354","8":"0.081655708"},{"Metabolite":"N,N-Dimethylglycine","1":"0.118562769","2":"0.06525518","3":"0.084481174","4":"0.06688979","5":"0.073589097","6":"0.023689393","7":"0.039858101","8":"0.062590802"},{"Metabolite":"Choline","1":"0.367262983","2":"0.451254009","3":"0.717078256","4":"0.770074421","5":"0.567509028","6":"0.632184827","7":"0.333604816","8":"0.33962248"},{"Metabolite":"Ser","1":"2.553815995","2":"2.442594057","3":"2.237259314","4":"2.035316905","5":"2.077105419","6":"1.764300838","7":"1.777318948","8":"2.238158939"},{"Metabolite":"Diethanolamine","1":"2.128404877","2":"2.144162213","3":"3.069870651","4":"2.124582807","5":"1.310565074","6":"1.109393474","7":"1.632300092","8":"2.128098762"},{"Metabolite":"Hypotaurine","1":"0.478364587","2":"0.803011705","3":"0.791642675","4":"0.763713391","5":"0.373422329","6":"0.331563899","7":"0.253968842","8":"0.46749539"},{"Metabolite":"Creatinine","1":"0.058914636","2":"0.045942911","3":"0.04569239","4":"0.046417411","5":"0.039474826","6":"0.028378118","7":"0.030274291","8":"0.042457626"},{"Metabolite":"Pro","1":"1.662722694","2":"1.67305686","3":"1.703526763","4":"1.632365867","5":"0.96160816","6":"0.946352331","7":"0.983706994","8":"1.099097583"},{"Metabolite":"Val","1":"3.007366305","2":"3.575068491","3":"3.605167358","4":"3.512489582","5":"3.430507297","6":"3.392016175","7":"3.352221005","8":"4.180537861"},{"Metabolite":"Betaine","1":"1.063455742","2":"0.690390439","3":"0.695347589","4":"0.410705985","5":"0.605767533","6":"0.237996882","7":"0.311965394","8":"0.30224697"},{"Metabolite":"Thr","1":"5.028270901","2":"5.464745754","3":"5.234471966","4":"5.318597061","5":"5.377011971","6":"5.153478742","7":"5.208624002","8":"6.674655272"},{"Metabolite":"Nicotinamide","1":"","2":"0.057060455","3":"0.052835894","4":"0.058409538","5":"0.046295827","6":"0.039921359","7":"0.032151968","8":"0.058263281"},{"Metabolite":"Taurine","1":"4.039529936","2":"5.799690707","3":"6.308111934","4":"4.96941524","5":"2.643010254","6":"2.476582045","7":"1.63839337","8":"3.184629953"},{"Metabolite":"Melamine","1":"0.120338655","2":"0.089148858","3":"0.120176708","4":"0.107212459","5":"0.06182528","6":"0.145787922","7":"0.243541601","8":"0.155652258"},{"Metabolite":"N-Acetylputrescine","1":"","2":"0.023521266","3":"","4":"0.021297206","5":"","6":"","7":"","8":"0.011870902"},{"Metabolite":"Hydroxyproline","1":"0.134303671","2":"0.148515488","3":"0.170133918","4":"0.132038471","5":"0.121546515","6":"0.103345969","7":"0.109197468","8":"0.134830522"},{"Metabolite":"Creatine","1":"3.165771963","2":"3.800925958","3":"3.927423091","4":"3.573655778","5":"2.485121434","6":"2.235946921","7":"2.135318225","8":"2.937185282"},{"Metabolite":"Ile","1":"2.664846041","2":"3.171271516","3":"3.288375529","4":"3.065452424","5":"3.071350506","6":"2.830898592","7":"2.864585223","8":"3.826531921"},{"Metabolite":"Leu","1":"2.966638856","2":"3.412940099","3":"3.630294925","4":"3.419757356","5":"3.21205273","6":"3.174380821","7":"2.998197104","8":"4.046900268"},{"Metabolite":"Asn","1":"0.348609527","2":"0.322770613","3":"0.26743227","4":"0.251721006","5":"0.358932757","6":"0.275753856","7":"0.434091097","8":"0.314085987"},{"Metabolite":"Ornithine","1":"0.254986716","2":"0.176100571","3":"0.118760003","4":"0.144998504","5":"0.136452136","6":"0.111980623","7":"0.148234202","8":"0.114712271"},{"Metabolite":"Asp","1":"4.232217107","2":"2.935866383","3":"3.020061998","4":"2.774175049","5":"1.394429558","6":"1.1461316","7":"1.184762989","8":"1.327901506"},{"Metabolite":"Adenine","1":"","2":"0.033447789","3":"","4":"","5":"0.025006858","6":"0.015120403","7":"0.014423314","8":""},{"Metabolite":"Hypoxanthine","1":"0.108165226","2":"0.131177005","3":"0.182610824","4":"0.122847278","5":"0.353050256","6":"0.473272118","7":"0.168821086","8":"0.138781383"},{"Metabolite":"1-Methylnicotinamide","1":"0.871521409","2":"0.942351165","3":"0.980234776","4":"0.891026884","5":"0.62026336","6":"0.656643935","7":"0.656639539","8":"0.738272075"},{"Metabolite":"gamma-Butyrobetaine","1":"0.037498862","2":"0.03458596","3":"0.035482326","4":"0.038956251","5":"0.030588674","6":"0.019480533","7":"0.017894813","8":"0.029476631"},{"Metabolite":"Spermidine","1":"0.028866642","2":"0.044223679","3":"0.062465649","4":"0.041232428","5":"0.054743236","6":"0.038899701","7":"0.042641243","8":"0.068481627"},{"Metabolite":"Gln","1":"11.86731194","2":"13.79470797","3":"14.21096347","4":"13.61114176","5":"12.64509869","6":"11.33685768","7":"11.13115521","8":"16.3872217"},{"Metabolite":"Lys","1":"0.921790317","2":"0.956923343","3":"0.928672079","4":"0.935512448","5":"1.36645726","6":"1.401012408","7":"1.408355346","8":"1.366793275"},{"Metabolite":"Glu","1":"53.70985443","2":"61.43126239","3":"61.89405386","4":"60.83639432","5":"41.80081749","6":"38.97090501","7":"40.41951079","8":"56.40677805"},{"Metabolite":"Met","1":"0.680917653","2":"0.774293777","3":"0.841097833","4":"0.804167798","5":"0.803629842","6":"0.753961835","7":"0.728658267","8":"1.030278712"},{"Metabolite":"His","1":"0.918965195","2":"0.986424237","3":"0.960533941","4":"1.023192844","5":"1.019948108","6":"0.920241364","7":"0.968973347","8":"1.188823449"},{"Metabolite":"alpha-Aminoadipate","1":"0.071393592","2":"0.099408058","3":"0.111034111","4":"0.086501009","5":"0.051018826","6":"0.065703364","7":"0.045106664","8":"0.07299699"},{"Metabolite":"Carnitine","1":"0.293975864","2":"0.357996646","3":"0.350117623","4":"0.325760259","5":"0.224069794","6":"0.21260547","7":"0.200272041","8":"0.246107259"},{"Metabolite":"Methionine sulfoxide","1":"","2":"","3":"","4":"","5":"0.05840955","6":"0.054961023","7":"","8":"0.062301009"},{"Metabolite":"Phe","1":"1.568148022","2":"2.008496976","3":"1.925221044","4":"1.911854606","5":"1.923701438","6":"1.795124932","7":"1.742808639","8":"2.38391782"},{"Metabolite":"Pyridoxine","1":"0.034448336","2":"0.032699817","3":"0.035804231","4":"0.029015857","5":"0.025429137","6":"0.024670692","7":"0.025073267","8":"0.02258561"},{"Metabolite":"Arg","1":"0.467032003","2":"0.514221327","3":"0.484156951","4":"0.504673917","5":"0.649303314","6":"0.675458179","7":"0.581713942","8":"0.641211054"},{"Metabolite":"Citrulline","1":"0.065948498","2":"0.073829965","3":"0.06675738","4":"0.057707913","5":"0.057079732","6":"0.053840314","7":"0.06921747","8":"0.073168959"},{"Metabolite":"Tyr","1":"2.159812433","2":"2.566277348","3":"2.495540513","4":"2.568322856","5":"2.657822793","6":"2.512240854","7":"2.447661875","8":"3.215579554"},{"Metabolite":"Phosphorylcholine","1":"8.114472445","2":"11.90920018","3":"12.26064826","4":"12.10596344","5":"6.341062837","6":"5.77881079","7":"4.946170451","8":"7.904354878"},{"Metabolite":"N8-Acetylspermidine","1":"0.008703636","2":"0.009286308","3":"0.010350956","4":"0.009492139","5":"0.005430806","6":"0.006684086","7":"","8":"0.007707366"},{"Metabolite":"N1-Acetylspermidine","1":"0.008410101","2":"0.017891064","3":"0.016310758","4":"0.015304572","5":"0.02361775","6":"0.026113059","7":"0.01986271","8":"0.02735244"},{"Metabolite":"N6,N6,N6-Trimethyllysine","1":"0.012680247","2":"0.011166906","3":"0.015219006","4":"0.010218991","5":"0.026749322","6":"0.031248611","7":"0.028958429","8":"0.024524002"},{"Metabolite":"SAH","1":"","2":"0.029891219","3":"","4":"0.020446045","5":"0.016368559","6":"0.025045819","7":"0.014950691","8":"0.02308613"},{"Metabolite":"SAM+","1":"0.128026954","2":"0.172658215","3":"0.159974608","4":"0.162956361","5":"0.114686591","6":"0.126386376","7":"0.11246536","8":"0.126675185"},{"Metabolite":"o-Acetylcarnitine","1":"0.071327043","2":"0.087342238","3":"0.098187993","4":"0.074859897","5":"0.077796249","6":"0.064308985","7":"0.062159132","8":"0.076573166"},{"Metabolite":"Trp","1":"0.502545704","2":"0.543164044","3":"0.535634878","4":"0.55284259","5":"0.56571776","6":"0.546692203","7":"0.536969654","8":"0.739561788"},{"Metabolite":"Cysteine-glutathione disulphide","1":"","2":"","3":"","4":"","5":"","6":"0.121901058","7":"0.215574975","8":""},{"Metabolite":"N-Acetylglucosamine","1":"0.411240677","2":"0.337511777","3":"0.43776125","4":"0.333680089","5":"0.315903059","6":"0.3051426","7":"0.264198209","8":"0.272681157"},{"Metabolite":"Cystathionine","1":"0.222602385","2":"0.284125095","3":"0.261851181","4":"0.283354905","5":"0.213011465","6":"0.208580048","7":"0.223968843","8":"0.270283511"},{"Metabolite":"Thymidine","1":"2.566910102","2":"2.36895773","3":"2.29271609","4":"2.050853019","5":"1.449721691","6":"1.339567945","7":"1.64933367","8":"1.411886198"},{"Metabolite":"Cytidine","1":"0.044814771","2":"0.044743435","3":"0.036687463","4":"0.036377675","5":"0.063670556","6":"0.105166981","7":"0.068615101","8":"0.04916207"},{"Metabolite":"Uridine","1":"2.29297091","2":"2.190736746","3":"2.088087998","4":"1.788165589","5":"1.495809649","6":"1.016393036","7":"1.056118446","8":"1.168069507"},{"Metabolite":"gamma-Glu-cys","1":"0.186457955","2":"0.166947172","3":"0.201960597","4":"0.19798494","5":"0.114296312","6":"0.068372753","7":"0.099941637","8":"0.112966126"},{"Metabolite":"Glycerophosphorylcholine","1":"2.016734903","2":"1.063295134","3":"1.216620258","4":"1.243552367","5":"0.600713588","6":"0.564345723","7":"0.855136901","8":"0.541824863"},{"Metabolite":"Thiamine","1":"0.088634028","2":"0.096919273","3":"0.101119283","4":"0.087235946","5":"0.07983425","6":"0.080255995","7":"0.082990895","8":"0.094051486"},{"Metabolite":"Adenosine","1":"0.020896454","2":"0.022450367","3":"0.022282407","4":"0.017411758","5":"0.058337097","6":"0.070771957","7":"0.036332969","8":"0.023993546"},{"Metabolite":"Inosine","1":"0.07729817","2":"0.079278138","3":"0.094575498","4":"0.069654952","5":"0.278503878","6":"0.348666273","7":"0.161308439","8":"0.101302648"},{"Metabolite":"Guanosine","1":"","2":"","3":"","4":"","5":"0.024859778","6":"0.031115049","7":"0.033745464","8":"0.024320531"},{"Metabolite":"5-Methylthioadenosine","1":"0.023559458","2":"0.03044255","3":"0.025106682","4":"0.02582567","5":"0.017363105","6":"0.020152484","7":"0.016910062","8":"0.017550659"},{"Metabolite":"Glutathione(ox)","1":"6.705120937","2":"8.387508781","3":"3.991878385","4":"4.980242813","5":"5.928173427","6":"11.61722779","7":"7.290645044","8":"5.40720092"},{"Metabolite":"Glutathione(red)","1":"24.38206641","2":"37.82835869","3":"42.16592069","4":"42.92210204","5":"16.59842957","6":"6.363419055","7":"8.757296637","8":"26.0800522"},{"Metabolite":"S-Lactoylglutathione","1":"0.048996765","2":"","3":"0.064561023","4":"0.049860604","5":"","6":"0.145833733","7":"0.032950281","8":"0.081986104"}],

"Metabolites":[{"Metabolite":"Urea","KEGG ID":"C00086"},{"Metabolite":"Gly","KEGG ID":"C00037"},{"Metabolite":"Trimethylamine N-oxide","KEGG ID":"C01104"},{"Metabolite":"Putrescine(1,4-Butanediamine)","KEGG ID":"C00134"},{"Metabolite":"beta-Ala","KEGG ID":"C00099"},{"Metabolite":"Ala","KEGG ID":"C00041"},{"Metabolite":"Cyclohexylamine","KEGG ID":"C00571"},{"Metabolite":"GABA","KEGG ID":"C00334"},{"Metabolite":"2AB","KEGG ID":"C02356"},{"Metabolite":"N,N-Dimethylglycine","KEGG ID":"C01026"},{"Metabolite":"Choline","KEGG ID":"C00114"},{"Metabolite":"Ser","KEGG ID":"C00065"},{"Metabolite":"Diethanolamine","KEGG ID":"C06772"},{"Metabolite":"Hypotaurine","KEGG ID":"C00519"},{"Metabolite":"Creatinine","KEGG ID":"C00791"},{"Metabolite":"Pro","KEGG ID":"C00148"},{"Metabolite":"Val","KEGG ID":"C00183"},{"Metabolite":"Betaine","KEGG ID":"C00719"},{"Metabolite":"Thr","KEGG ID":"C00188"},{"Metabolite":"Nicotinamide","KEGG ID":"C00153"},{"Metabolite":"Taurine","KEGG ID":"C00245"},{"Metabolite":"Melamine","KEGG ID":"C08737"},{"Metabolite":"N-Acetylputrescine","KEGG ID":"C02714"},{"Metabolite":"Hydroxyproline","KEGG ID":"C01157"},{"Metabolite":"Creatine","KEGG ID":"C00300"},{"Metabolite":"Ile","KEGG ID":"C00407"},{"Metabolite":"Leu","KEGG ID":"C00123"},{"Metabolite":"Asn","KEGG ID":"C00152"},{"Metabolite":"Ornithine","KEGG ID":"C00077"},{"Metabolite":"Asp","KEGG ID":"C00049"},{"Metabolite":"Adenine","KEGG ID":"C00147"},{"Metabolite":"Hypoxanthine","KEGG ID":"C00262"},{"Metabolite":"1-Methylnicotinamide","KEGG ID":"C02918"},{"Metabolite":"gamma-Butyrobetaine","KEGG ID":"C01181"},{"Metabolite":"Spermidine","KEGG ID":"C00315"},{"Metabolite":"Gln","KEGG ID":"C00064"},{"Metabolite":"Lys","KEGG ID":"C00047"},{"Metabolite":"Glu","KEGG ID":"C00025"},{"Metabolite":"Met","KEGG ID":"C00073"},{"Metabolite":"His","KEGG ID":"C00135"},{"Metabolite":"alpha-Aminoadipate","KEGG ID":"C00956"},{"Metabolite":"Carnitine","KEGG ID":"C00318"},{"Metabolite":"Methionine sulfoxide","KEGG ID":"C02989"},{"Metabolite":"Phe","KEGG ID":"C00079"},{"Metabolite":"Pyridoxine","KEGG ID":"C00314"},{"Metabolite":"Arg","KEGG ID":"C00062"},{"Metabolite":"Citrulline","KEGG ID":"C00327"},{"Metabolite":"Tyr","KEGG ID":"C00082"},{"Metabolite":"Phosphorylcholine","KEGG ID":"C00588"},{"Metabolite":"N8-Acetylspermidine","KEGG ID":"C01029"},{"Metabolite":"N1-Acetylspermidine","KEGG ID":"C00612"},{"Metabolite":"N6,N6,N6-Trimethyllysine","KEGG ID":"C03793"},{"Metabolite":"SAH","KEGG ID":"C00021"},{"Metabolite":"SAM+","KEGG ID":"C00019"},{"Metabolite":"o-Acetylcarnitine","KEGG ID":"C02571"},{"Metabolite":"Trp","KEGG ID":"C00078"},{"Metabolite":"Cysteine-glutathione disulphide","KEGG ID":"-"},{"Metabolite":"N-Acetylglucosamine","KEGG ID":"C00140"},{"Metabolite":"Cystathionine","KEGG ID":"C02291"},{"Metabolite":"Thymidine","KEGG ID":"C00214"},{"Metabolite":"Cytidine","KEGG ID":"C00475"},{"Metabolite":"Uridine","KEGG ID":"C00299"},{"Metabolite":"gamma-Glu-cys","KEGG ID":"C00669"},{"Metabolite":"Glycerophosphorylcholine","KEGG ID":"C00670"},{"Metabolite":"Thiamine","KEGG ID":"C00378"},{"Metabolite":"Adenosine","KEGG ID":"C00212"},{"Metabolite":"Inosine","KEGG ID":"C00294"},{"Metabolite":"Guanosine","KEGG ID":"C00387"},{"Metabolite":"5-Methylthioadenosine","KEGG ID":"C00170"},{"Metabolite":"Glutathione(ox)","KEGG ID":"C00127"},{"Metabolite":"Glutathione(red)","KEGG ID":"C00051"},{"Metabolite":"S-Lactoylglutathione","KEGG ID":"C03451"}]
}

}