#METABOLOMICS WORKBENCH wpathmasiri_20140228_9161061_mwtab.txt DATATRACK_ID:21 TEXT OUTPUT STUDY_ID:ST000020 ANALYSIS_ID:AN000039 PROJECT_ID:PR000019 VERSION 1 CREATED_ON 10-02-2015 #PROJECT PR:PROJECT_TITLE Intensive Diet and Exercise for Arthritis (IDEA) trial PR:PROJECT_TYPE Effects of a calorie-restricting diet, exercise, and a combination of both in PR:PROJECT_TYPE reducing knee inflammation and compressive forces in obese and overweight adults PR:PROJECT_TYPE with knee osteoarthritis (OA). PR:PROJECT_SUMMARY Osteoarthritis (OA) is the most common form of arthritis and the number one PR:PROJECT_SUMMARY cause of chronic disability in adults. Interventions which can stop or even slow PR:PROJECT_SUMMARY the progression of OA are badly needed. The long-term goal of this proposal is PR:PROJECT_SUMMARY to use a metabolomics approach to gain a better understanding of metabolic PR:PROJECT_SUMMARY alterations in people with knee osteoarthritis (OA) and to discover novel PR:PROJECT_SUMMARY biomarkers of disease activity. OA results from progressive destruction of the PR:PROJECT_SUMMARY articular cartilage accompanied by hypertrophic changes in adjacent bone, PR:PROJECT_SUMMARY varying degrees of synovial inflammation, and destruction of other joint PR:PROJECT_SUMMARY tissues, including the menisci and ligaments. There are multiple risk factors PR:PROJECT_SUMMARY for OA, including age and joint injury, but perhaps most relevant to this PR:PROJECT_SUMMARY proposal is the role of obesity. The mechanism by which altered metabolism in PR:PROJECT_SUMMARY certain obese individuals might contribute to OA pathogenesis is poorly PR:PROJECT_SUMMARY understood and will be a subject of this proposal. A second critical need in the PR:PROJECT_SUMMARY OA field, which will be addressed by this study, is the availability of PR:PROJECT_SUMMARY biomarkers to detect early disease, predict which people are more likely to PR:PROJECT_SUMMARY progress, or measure response to therapy. Our general hypothesis for this pilot PR:PROJECT_SUMMARY study is that metabolic differences, measured by a metabolomic analysis of urine PR:PROJECT_SUMMARY samples, can be used to distinguish overweight and obese individuals who develop PR:PROJECT_SUMMARY symptomatic knee OA from those who do not and distinguish those with OA who will PR:PROJECT_SUMMARY progress. To test this hypothesis our specific aims are to: 1) Determine if PR:PROJECT_SUMMARY metabolic differences, detected by a comprehensive metabolomics analysis, can be PR:PROJECT_SUMMARY used to distinguish people who will develop symptomatic knee OA from those who PR:PROJECT_SUMMARY will not and 2) Determine if metabolic differences, detected by a comprehensive PR:PROJECT_SUMMARY metabolomics analysis, distinguish people with knee OA who exhibit radiographic PR:PROJECT_SUMMARY progression during an 18 month exercise and weight loss intervention from those PR:PROJECT_SUMMARY who do not. PR:INSTITUTE Wake Forest University PR:DEPARTMENT Molecular Medicine, School of Medicine PR:LABORATORY Loeser Laboratory PR:LAST_NAME Richard PR:FIRST_NAME Loeser PR:ADDRESS Medical Center Blvd, Winsten-Salem, NC PR:EMAIL rloeser@wakehealth.edu PR:PHONE (336) 716-4322 PR:FUNDING_SOURCE NIH #STUDY ST:STUDY_TITLE Biomarker Discovery in Knee Osteoarthritis (I) ST:STUDY_TYPE Biomarker Discovery in Knee Osteoarthritis ST:STUDY_SUMMARY The goal of the study was to determine whether there is a set of metabolites ST:STUDY_SUMMARY that differentiate people who have knee OA and show radiographic disease ST:STUDY_SUMMARY progression over 18 months from those who have knee OA and do not show disease ST:STUDY_SUMMARY progression over the same time period. ST:INSTITUTE RTI International ST:DEPARTMENT Systems and Translational Sciences (STS) ST:LABORATORY NIH Eastern Regional Comprehensive Metabolomics Resource Core (RTI RCMRC) ST:LAST_NAME Sumner ST:FIRST_NAME Susan ST:ADDRESS 3040 East Cornwallis Road, Research Triangle Park, NC 27709, USA. ST:EMAIL ssumner@rti.org ST:PHONE 919-541-7479 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 88 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:AGE_OR_AGE_RANGE 56-78 SU:WEIGHT_OR_WEIGHT_RANGE Obese or Overweight SU:GENDER Male, Female SU:HUMAN_RACE Caucasian, African American SU:HUMAN_TRIAL_TYPE Intervention SU:SPECIES_GROUP Human #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 1338-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:B | Gender:M SUBJECT_SAMPLE_FACTORS - 1380-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2370-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2925-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3163-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3166-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3727-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1401-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 1538-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 2120-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 3994-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 4272-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet Only | Race:B | Gender:F SUBJECT_SAMPLE_FACTORS - 1882-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet Only | Race:B | Gender:M SUBJECT_SAMPLE_FACTORS - 1382-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1702-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1864-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2479-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3130-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3296-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3285-fu18 Time Point:fu18 | Progressor:N | Intervention:Diet Only | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 2944-fu18 Time Point:fu18 | Progressor:N | Intervention:Exer Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3786-fu18 Time Point:fu18 | Progressor:N | Intervention:Exer Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3612-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet&Exer | Race:B | Gender:F SUBJECT_SAMPLE_FACTORS - 1934-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2012-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2095-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3985-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1291-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 1441-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 1681-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 1808-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 2830-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet Only | Race:B | Gender:F SUBJECT_SAMPLE_FACTORS - 2276-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2924-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3331-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3706-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3750-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3929-fu18 Time Point:fu18 | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1745-fu18 Time Point:fu18 | Progressor:P | Intervention:Exer Only | Race:B | Gender:F SUBJECT_SAMPLE_FACTORS - 1488-fu18 Time Point:fu18 | Progressor:P | Intervention:Exer Only | Race:B | Gender:M SUBJECT_SAMPLE_FACTORS - 1106-fu18 Time Point:fu18 | Progressor:P | Intervention:Exer Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 4097-fu18 Time Point:fu18 | Progressor:P | Intervention:Exer Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1624-fu18 Time Point:fu18 | Progressor:P | Intervention:Exer Only | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 3845-fu18 Time Point:fu18 | Progressor:P | Intervention:Exer Only | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 1338-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:B | Gender:M SUBJECT_SAMPLE_FACTORS - 1380-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2370-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2925-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3163-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3166-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3727-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1401-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 1538-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 2120-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 3994-RV Time Point:RV | Progressor:N | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 4272-RV Time Point:RV | Progressor:N | Intervention:Diet Only | Race:B | Gender:F SUBJECT_SAMPLE_FACTORS - 1882-RV Time Point:RV | Progressor:N | Intervention:Diet Only | Race:B | Gender:M SUBJECT_SAMPLE_FACTORS - 1382-RV Time Point:RV | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1702-RV Time Point:RV | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1864-RV Time Point:RV | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2479-RV Time Point:RV | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3130-RV Time Point:RV | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3296-RV Time Point:RV | Progressor:N | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3285-RV Time Point:RV | Progressor:N | Intervention:Diet Only | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 2944-RV Time Point:RV | Progressor:N | Intervention:Exer Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3786-RV Time Point:RV | Progressor:N | Intervention:Exer Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3612-RV Time Point:RV | Progressor:P | Intervention:Diet&Exer | Race:B | Gender:F SUBJECT_SAMPLE_FACTORS - 1934-RV Time Point:RV | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2012-RV Time Point:RV | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2095-RV Time Point:RV | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3985-RV Time Point:RV | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1291-RV Time Point:RV | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 1441-RV Time Point:RV | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 1681-RV Time Point:RV | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 1808-RV Time Point:RV | Progressor:P | Intervention:Diet&Exer | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 2830-RV Time Point:RV | Progressor:P | Intervention:Diet Only | Race:B | Gender:F SUBJECT_SAMPLE_FACTORS - 2276-RV Time Point:RV | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 2924-RV Time Point:RV | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3331-RV Time Point:RV | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3706-RV Time Point:RV | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3750-RV Time Point:RV | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 3929-RV Time Point:RV | Progressor:P | Intervention:Diet Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1745-RV Time Point:RV | Progressor:P | Intervention:Exer Only | Race:B | Gender:F SUBJECT_SAMPLE_FACTORS - 1488-RV Time Point:RV | Progressor:P | Intervention:Exer Only | Race:B | Gender:M SUBJECT_SAMPLE_FACTORS - 1106-RV Time Point:RV | Progressor:P | Intervention:Exer Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 4097-RV Time Point:RV | Progressor:P | Intervention:Exer Only | Race:W | Gender:F SUBJECT_SAMPLE_FACTORS - 1624-RV Time Point:RV | Progressor:P | Intervention:Exer Only | Race:W | Gender:M SUBJECT_SAMPLE_FACTORS - 3845-RV Time Point:RV | Progressor:P | Intervention:Exer Only | Race:W | Gender:M #COLLECTION CO:COLLECTION_SUMMARY Urine samples (early morning [second morning void) after a 10-hour fast]) CO:COLLECTION_SUMMARY collected from 44 overweight or obese participants in the IDEA trial at baseline CO:COLLECTION_SUMMARY and at 18 months of follow-up were selected from two subgroups (n=22 each): a CO:COLLECTION_SUMMARY group that exhibited radiographic progression and an age, race, sex, and BMI CO:COLLECTION_SUMMARY matched group who did not progress. Progression was defined radiographically by CO:COLLECTION_SUMMARY a ? 0.7mm decrease in medial JSW from baseline to 18 months while CO:COLLECTION_SUMMARY non-progression was defined as a decrease in medial JSW of ?0.35mm . All CO:COLLECTION_SUMMARY subjects experienced knee pain and had radiographic evidence of tibiofemoral OA. CO:COLLECTION_SUMMARY For those people who were assigned an intervention that included exercise, the CO:COLLECTION_SUMMARY 18-month samples were collected at least 24 hours after the last acute bout of CO:COLLECTION_SUMMARY exercise training. In addition sampling was postponed (1-2 weeks after recovery CO:COLLECTION_SUMMARY from symptoms) in the event of an acute respiratory, urinary tract, or other CO:COLLECTION_SUMMARY infection. Urine was aliquoted and stored in cryovials at -80°C. CO:SAMPLE_TYPE Urine CO:COLLECTION_FREQUENCY Baseline and after 18 month (Follow up) CO:VOLUMEORAMOUNT_COLLECTED 1.8 mL of urine CO:STORAGE_CONDITIONS 80C. #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Chenomx Internal Standard solution (70 ul) and 230 ul D20 was added to each of SP:SAMPLEPREP_SUMMARY the 88 urine sample (400 ul), vortexed for 30s, and centrifuged at 12000 rcf for SP:SAMPLEPREP_SUMMARY 5min. Chenomx ISTD (Chenomx, Edmonton, Alberta, Canada) contains 5mM SP:SAMPLEPREP_SUMMARY 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, Chemical Shift Indicator), 100 SP:SAMPLEPREP_SUMMARY mM Imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) in D2O. SP:SAMPLEPREP_SUMMARY 600 µl aliquot of the supernatant was transferred into 5mm NMR tubes SP:SAMPLEPREP_SUMMARY (Bruker-Biospin, Germany). Phenotypic pooled urine samples were made by SP:SAMPLEPREP_SUMMARY combining 200 µl aliquots from each of the study samples belonging to the same SP:SAMPLEPREP_SUMMARY phenotype (Progressors-Baseline, Progressors-follow up, SP:SAMPLEPREP_SUMMARY Non-progressors-baseline, and Non-progressors-follow up). In addition, a SP:SAMPLEPREP_SUMMARY combined phenotypic pooled sample was also prepared by using 500 µl aliquot SP:SAMPLEPREP_SUMMARY from each of the phenotypic pooled sample. Pooled NMR samples were prepared as SP:SAMPLEPREP_SUMMARY described above and used as quality check (QC) samples. SP:SAMPLEPREP_PROTOCOL_ID RTI NMR SERUM CHENOMX method SP:SAMPLEPREP_PROTOCOL_FILENAME RTI_WOA_IDEA_Metabolomics_Procedure_Feb28_2014.docx SP:PROCESSING_METHOD Dilution using a mixture of D2O and Chenomx ISTD SP:PROCESSING_STORAGE_CONDITIONS On ice SP:EXTRACTION_METHOD None #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:LABORATORY_NAME RTI/DHMRI AN:ANALYSIS_TYPE NMR AN:ACQUISITION_DATE 2013-07-17 AN:SOFTWARE_VERSION Topspin/ 3.0 AN:OPERATOR_NAME Wimal Pathmasiri, Kevin Knagge AN:DATA_FORMAT fid, 1r #NMR NM:INSTRUMENT_TYPE Bruker Avance III NM:NMR_EXPERIMENT_TYPE 1D 1H NM:FIELD_FREQUENCY_LOCK Deuterium NM:STANDARD_CONCENTRATION 0.5 mM NM:SPECTROMETER_FREQUENCY 950 MHz NM:NMR_PROBE cryo, inverse ATMA NM:NMR_SOLVENT D2O NM:NMR_TUBE_SIZE 5mm x 7 in NM:SHIMMING_METHOD topshim NM:PULSE_SEQUENCE noesypr1d NM:WATER_SUPPRESSION presat NM:PULSE_WIDTH 12.15 us NM:POWER_LEVEL 12.589w NM:RECEIVER_GAIN 4 NM:OFFSET_FREQUENCY 4469.3 Hz NM:CHEMICAL_SHIFT_REF_CPD DSS NM:TEMPERATURE 298 K NM:NUMBER_OF_SCANS 64 NM:DUMMY_SCANS 4 NM:ACQUISITION_TIME 0.865 sec NM:RELAXATION_DELAY 2 sec NM:SPECTRAL_WIDTH 18939.40 Hz NM:NUM_DATA_POINTS_ACQUIRED 32K NM:REAL_DATA_POINTS 64K NM:LINE_BROADENING 0.5 NM:ZERO_FILLING yes NM:APODIZATION lorentzian NM:BASELINE_CORRECTION_METHOD Polynomial NM:CHEMICAL_SHIFT_REF_STD DSS NM:INSTRUMENT_NAME Bruker Avance III #NMR_METABOLITE_DATA NMR_METABOLITE_DATA:UNITS N/A NMR_METABOLITE_DATA_START Samples 1338-fu18 Factors Time Point:fu18 | Progressor:N | Intervention:Diet&Exer | Race:B | Gender:M no data NMR_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name no data METABOLITES_END #END