#METABOLOMICS WORKBENCH sdasari_20150305_9125171_mwtab.txt DATATRACK_ID:214 STUDY_ID:ST000149 ANALYSIS_ID:AN000236 PROJECT_ID:PR000130 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE High insulin combined with essential amino acids stimulates skeletal muscle PR:PROJECT_TITLE protein synthesis while decreasing insulin sensitivity in healthy humans PR:PROJECT_TYPE Targeted metabolomics PR:PROJECT_SUMMARY CONTEXT:Insulin and essential amino acids (EAAs) regulate skeletal muscle PR:PROJECT_SUMMARY synthesis, yet their independent effects on mitochondrial protein synthesis PR:PROJECT_SUMMARY and oxidative function remain to be clearly defined. OBJECTIVE:The purpose PR:PROJECT_SUMMARY this study was to determine the effects of high or low insulin with or without PR:PROJECT_SUMMARY on MiPS. DESIGN:Thirty participants were randomized to 3 groups of 10 each PR:PROJECT_SUMMARY each participant studied twice. Study groups comprised (1) low and high PR:PROJECT_SUMMARY (2) low insulin with and without EAAs, and (3) high insulin with and without PR:PROJECT_SUMMARY study was conducted in an in-patient clinical research PR:PROJECT_SUMMARY participants were 18 to 45 years old, had a body mass index of <25 kg/m(2), and PR:PROJECT_SUMMARY free of diseases and medications that might impair mitochondrial PR:PROJECT_SUMMARY (? 6 ?U/mL) and high (? 40 ?U/mL) insulin levels were maintained by iv insulin PR:PROJECT_SUMMARY during a somatostatin clamp while maintaining euglycemia (4.7-5.2 mM) and PR:PROJECT_SUMMARY GH and glucagon. The EAA infusion was 5.4% NephrAmine. PR:PROJECT_SUMMARY was infused, and muscle needle biopsies were performed. MAIN OUTCOMES:Muscle PR:PROJECT_SUMMARY oxidative enzymes, and plasma amino acid metabolites were PR:PROJECT_SUMMARY and oxidative enzyme activities did not differ between low and high insulin PR:PROJECT_SUMMARY 0.07 ± 0.009 vs 0.07 ± 0.006%/h, P = .86) or between EAAs and saline during PR:PROJECT_SUMMARY insulin (MiPS: 0.05 ± 0.01 vs 0.07 ± 0.01, P = .5). During high insulin, EAAs PR:PROJECT_SUMMARY comparison with saline increased MiPS (0.1 ± 0.01 vs 0.06 ± 0.01, P < .05) PR:PROJECT_SUMMARY cytochrome c oxidase activity (P < .05) but not citrate synthase (P = .27). EAA PR:PROJECT_SUMMARY decreased (P < .05) the glucose infusion rates needed to maintain euglycemia PR:PROJECT_SUMMARY low (? 40%) and high insulin (? 24%). CONCLUSION:EAAs increased MiPS and PR:PROJECT_SUMMARY enzyme activity only with high insulin concentrations. PR:INSTITUTE Mayo Clinic PR:DEPARTMENT Endocrinology PR:LABORATORY Dr. Sreekumaran Nair's lab PR:LAST_NAME Nair PR:FIRST_NAME Sreekumaran PR:ADDRESS Mayo Clinic, 200 First Street SW, Rochester, MN PR:EMAIL Dasari.Surendra@mayo.edu PR:PHONE - PR:FUNDING_SOURCE 5T32DK007352, 5R01DK041973, UL1 TR000135, U24DK100469 PR:PROJECT_COMMENTS 25222757 #STUDY ST:STUDY_TITLE High Insulin Combined With Essential Amino Acids Stimulates Skeletal Muscle ST:STUDY_TITLE Protein Synthesis While Decreasing Insulin Sensitivity in Healthy Humans ST:STUDY_TYPE High and low insulin with and without essential amino acids ST:STUDY_SUMMARY Thirty participants were randomized to 3 groups of 10 each with each ST:STUDY_SUMMARY studied twice. Study groups comprised (1) low and high insulin, (2) low insulin ST:STUDY_SUMMARY and without EAAs, and (3) high insulin with and without EAAs. ST:INSTITUTE Mayo Clinic ST:DEPARTMENT Endocrinology ST:LAST_NAME Nair ST:FIRST_NAME Sreekumaran ST:ADDRESS - ST:EMAIL Dasari.Surendra@mayo.edu ST:PHONE - ST:NUM_GROUPS 3 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS 10 10C Sample Group:High Insulin Study Date=07/22/08 SUBJECT_SAMPLE_FACTORS 10 10D Sample Group:High Insulin Study Date=07/22/08 SUBJECT_SAMPLE_FACTORS 1 1C Sample Group:High Insulin Study Date=01/05/07 SUBJECT_SAMPLE_FACTORS 1 1D Sample Group:High Insulin Study Date=01/05/07 SUBJECT_SAMPLE_FACTORS 2 2C Sample Group:High Insulin Study Date=01/09/07 SUBJECT_SAMPLE_FACTORS 2 2D Sample Group:High Insulin Study Date=01/09/07 SUBJECT_SAMPLE_FACTORS 3 3C Sample Group:High Insulin Study Date=03/23/07 SUBJECT_SAMPLE_FACTORS 3 3D Sample Group:High Insulin Study Date=03/23/07 SUBJECT_SAMPLE_FACTORS 4 4C Sample Group:High Insulin Study Date=11/30/07 SUBJECT_SAMPLE_FACTORS 4 4D Sample Group:High Insulin Study Date=11/30/07 SUBJECT_SAMPLE_FACTORS 5 5C Sample Group:High Insulin Study Date=01/10/08 SUBJECT_SAMPLE_FACTORS 5 5D Sample Group:High Insulin Study Date=01/10/08 SUBJECT_SAMPLE_FACTORS 6 6C Sample Group:High Insulin Study Date=02/19/08 SUBJECT_SAMPLE_FACTORS 6 6D Sample Group:High Insulin Study Date=02/19/08 SUBJECT_SAMPLE_FACTORS 7 7C Sample Group:High Insulin Study Date=04/03/08 SUBJECT_SAMPLE_FACTORS 7 7D Sample Group:High Insulin Study Date=04/03/08 SUBJECT_SAMPLE_FACTORS 8 8C Sample Group:High Insulin Study Date=07/23/08 SUBJECT_SAMPLE_FACTORS 8 8D Sample Group:High Insulin Study Date=07/23/08 SUBJECT_SAMPLE_FACTORS 9 9C Sample Group:High Insulin Study Date=06/19/08 SUBJECT_SAMPLE_FACTORS 9 9D Sample Group:High Insulin Study Date=06/19/08 SUBJECT_SAMPLE_FACTORS 20 20C Sample Group:High Insulin + EAA Study Date=05/22/07 SUBJECT_SAMPLE_FACTORS 20 20D Sample Group:High Insulin + EAA Study Date=05/22/07 SUBJECT_SAMPLE_FACTORS 21 21C Sample Group:High Insulin + EAA Study Date=06/01/07 SUBJECT_SAMPLE_FACTORS 21 21D Sample Group:High Insulin + EAA Study Date=06/01/07 SUBJECT_SAMPLE_FACTORS 22 22C Sample Group:High Insulin + EAA Study Date=01/08/08 SUBJECT_SAMPLE_FACTORS 22 22D Sample Group:High Insulin + EAA Study Date=01/08/08 SUBJECT_SAMPLE_FACTORS 23 23C Sample Group:High Insulin + EAA Study Date=03/19/08 SUBJECT_SAMPLE_FACTORS 23 23D Sample Group:High Insulin + EAA Study Date=03/19/08 SUBJECT_SAMPLE_FACTORS 24 24C Sample Group:High Insulin + EAA Study Date=03/17/08 SUBJECT_SAMPLE_FACTORS 24 24D Sample Group:High Insulin + EAA Study Date=03/17/08 SUBJECT_SAMPLE_FACTORS 25 25C Sample Group:High Insulin + EAA Study Date=04/24/08 SUBJECT_SAMPLE_FACTORS 25 25D Sample Group:High Insulin + EAA Study Date=04/24/08 SUBJECT_SAMPLE_FACTORS 26 26C Sample Group:High Insulin + EAA Study Date=07/21/08 SUBJECT_SAMPLE_FACTORS 26 26D Sample Group:High Insulin + EAA Study Date=07/21/08 SUBJECT_SAMPLE_FACTORS 27 27C Sample Group:High Insulin + EAA Study Date=08/15/08 SUBJECT_SAMPLE_FACTORS 27 27D Sample Group:High Insulin + EAA Study Date=08/15/08 SUBJECT_SAMPLE_FACTORS 28 28C Sample Group:High Insulin + EAA Study Date=09/25/08 SUBJECT_SAMPLE_FACTORS 29 28D Sample Group:High Insulin + EAA Study Date=09/25/08 SUBJECT_SAMPLE_FACTORS 28 29A Sample Group:High Insulin + EAA Study Date=08/19/08 SUBJECT_SAMPLE_FACTORS 29 29D Sample Group:High Insulin + EAA Study Date=10/24/08 SUBJECT_SAMPLE_FACTORS 20 20A Sample Group:High Insulin+Saline Study Date=03/27/07 SUBJECT_SAMPLE_FACTORS 20 20B Sample Group:High Insulin+Saline Study Date=03/27/07 SUBJECT_SAMPLE_FACTORS 21 21A Sample Group:High Insulin+Saline Study Date=05/10/07 SUBJECT_SAMPLE_FACTORS 21 21B Sample Group:High Insulin+Saline Study Date=05/10/07 SUBJECT_SAMPLE_FACTORS 22 22A Sample Group:High Insulin+Saline Study Date=12/11/07 SUBJECT_SAMPLE_FACTORS 22 22B Sample Group:High Insulin+Saline Study Date=12/11/07 SUBJECT_SAMPLE_FACTORS 23 23A Sample Group:High Insulin+Saline Study Date=02/27/08 SUBJECT_SAMPLE_FACTORS 23 23B Sample Group:High Insulin+Saline Study Date=02/27/08 SUBJECT_SAMPLE_FACTORS 24 24A Sample Group:High Insulin+Saline Study Date=03/03/08 SUBJECT_SAMPLE_FACTORS 24 24B Sample Group:High Insulin+Saline Study Date=03/03/08 SUBJECT_SAMPLE_FACTORS 25 25A Sample Group:High Insulin+Saline Study Date=03/14/08 SUBJECT_SAMPLE_FACTORS 25 25B Sample Group:High Insulin+Saline Study Date=03/14/08 SUBJECT_SAMPLE_FACTORS 26 26A Sample Group:High Insulin+Saline Study Date=04/23/08 SUBJECT_SAMPLE_FACTORS 26 26B Sample Group:High Insulin+Saline Study Date=04/23/08 SUBJECT_SAMPLE_FACTORS 27 27A Sample Group:High Insulin+Saline Study Date=07/10/08 SUBJECT_SAMPLE_FACTORS 27 27B Sample Group:High Insulin+Saline Study Date=07/10/08 SUBJECT_SAMPLE_FACTORS 28 28A Sample Group:High Insulin+Saline Study Date=08/04/08 SUBJECT_SAMPLE_FACTORS 28 28B Sample Group:High Insulin+Saline Study Date=08/04/08 SUBJECT_SAMPLE_FACTORS 29 29B Sample Group:High Insulin+Saline Study Date=08/19/08 SUBJECT_SAMPLE_FACTORS 29 29C Sample Group:High Insulin+Saline Study Date=10/24/08 SUBJECT_SAMPLE_FACTORS 11 11C Sample Group:Low Ins +EAA Study Date=01/04/07 SUBJECT_SAMPLE_FACTORS 11 11D Sample Group:Low Ins +EAA Study Date=01/04/07 SUBJECT_SAMPLE_FACTORS 12 12C Sample Group:Low Ins +EAA Study Date=03/02/07 SUBJECT_SAMPLE_FACTORS 12 12D Sample Group:Low Ins +EAA Study Date=03/02/07 SUBJECT_SAMPLE_FACTORS 13 13C Sample Group:Low Ins +EAA Study Date=02/20/07 SUBJECT_SAMPLE_FACTORS 13 13D Sample Group:Low Ins +EAA Study Date=02/20/07 SUBJECT_SAMPLE_FACTORS 14 14C Sample Group:Low Ins +EAA Study Date=12/04/07 SUBJECT_SAMPLE_FACTORS 14 14D Sample Group:Low Ins +EAA Study Date=12/04/07 SUBJECT_SAMPLE_FACTORS 15 15C Sample Group:Low Ins +EAA Study Date=02/29/08 SUBJECT_SAMPLE_FACTORS 15 15D Sample Group:Low Ins +EAA Study Date=02/29/08 SUBJECT_SAMPLE_FACTORS 16 16C Sample Group:Low Ins +EAA Study Date=03/05/08 SUBJECT_SAMPLE_FACTORS 16 16D Sample Group:Low Ins +EAA Study Date=03/05/08 SUBJECT_SAMPLE_FACTORS 17 17C Sample Group:Low Ins +EAA Study Date=04/25/08 SUBJECT_SAMPLE_FACTORS 17 17D Sample Group:Low Ins +EAA Study Date=04/25/08 SUBJECT_SAMPLE_FACTORS 18 18C Sample Group:Low Ins +EAA Study Date=06/26/08 SUBJECT_SAMPLE_FACTORS 18 18D Sample Group:Low Ins +EAA Study Date=06/26/08 SUBJECT_SAMPLE_FACTORS 19 19C Sample Group:Low Ins +EAA Study Date=07/31/08 SUBJECT_SAMPLE_FACTORS 19 19D Sample Group:Low Ins +EAA Study Date=07/31/08 SUBJECT_SAMPLE_FACTORS 30 30C Sample Group:Low Ins +EAA Study Date=12/05/07 SUBJECT_SAMPLE_FACTORS 30 30D Sample Group:Low Ins +EAA Study Date=12/05/07 SUBJECT_SAMPLE_FACTORS 11 11A Sample Group:Low Ins+ Saline Study Date=12/06/06 SUBJECT_SAMPLE_FACTORS 11 11B Sample Group:Low Ins+ Saline Study Date=12/06/06 SUBJECT_SAMPLE_FACTORS 12 12A Sample Group:Low Ins+ Saline Study Date=01/26/07 SUBJECT_SAMPLE_FACTORS 12 12B Sample Group:Low Ins+ Saline Study Date=01/26/07 SUBJECT_SAMPLE_FACTORS 13 13A Sample Group:Low Ins+ Saline Study Date=01/30/07 SUBJECT_SAMPLE_FACTORS 13 13B Sample Group:Low Ins+ Saline Study Date=01/30/07 SUBJECT_SAMPLE_FACTORS 14 14A Sample Group:Low Ins+ Saline Study Date=11/15/07 SUBJECT_SAMPLE_FACTORS 14 14B Sample Group:Low Ins+ Saline Study Date=11/15/07 SUBJECT_SAMPLE_FACTORS 15 15A Sample Group:Low Ins+ Saline Study Date=12/06/07 SUBJECT_SAMPLE_FACTORS 15 15B Sample Group:Low Ins+ Saline Study Date=12/06/07 SUBJECT_SAMPLE_FACTORS 16 16A Sample Group:Low Ins+ Saline Study Date=02/13/08 SUBJECT_SAMPLE_FACTORS 16 16B Sample Group:Low Ins+ Saline Study Date=02/13/08 SUBJECT_SAMPLE_FACTORS 17 17A Sample Group:Low Ins+ Saline Study Date=03/12/08 SUBJECT_SAMPLE_FACTORS 17 17B Sample Group:Low Ins+ Saline Study Date=03/12/08 SUBJECT_SAMPLE_FACTORS 18 18A Sample Group:Low Ins+ Saline Study Date=04/22/08 SUBJECT_SAMPLE_FACTORS 18 18B Sample Group:Low Ins+ Saline Study Date=04/22/08 SUBJECT_SAMPLE_FACTORS 19 19A Sample Group:Low Ins+ Saline Study Date=07/09/08 SUBJECT_SAMPLE_FACTORS 19 19B Sample Group:Low Ins+ Saline Study Date=07/09/08 SUBJECT_SAMPLE_FACTORS 30 30A Sample Group:Low Ins+ Saline Study Date=11/07/07 SUBJECT_SAMPLE_FACTORS 30 30B Sample Group:Low Ins+ Saline Study Date=11/07/07 SUBJECT_SAMPLE_FACTORS 10 10A Sample Group:Low Insulin Study Date=07/02/08 SUBJECT_SAMPLE_FACTORS 10 10B Sample Group:Low Insulin Study Date=07/02/08 SUBJECT_SAMPLE_FACTORS 1 1A Sample Group:Low Insulin Study Date=12/13/06 SUBJECT_SAMPLE_FACTORS 1 1B Sample Group:Low Insulin Study Date=12/13/06 SUBJECT_SAMPLE_FACTORS 2 2A Sample Group:Low Insulin Study Date=12/20/06 SUBJECT_SAMPLE_FACTORS 2 2B Sample Group:Low Insulin Study Date=12/20/06 SUBJECT_SAMPLE_FACTORS 3 3A Sample Group:Low Insulin Study Date=01/26/07 SUBJECT_SAMPLE_FACTORS 3 3B Sample Group:Low Insulin Study Date=01/26/07 SUBJECT_SAMPLE_FACTORS 4 4A Sample Group:Low Insulin Study Date=11/09/07 SUBJECT_SAMPLE_FACTORS 4 4B Sample Group:Low Insulin Study Date=11/09/07 SUBJECT_SAMPLE_FACTORS 5 5A Sample Group:Low Insulin Study Date=12/21/07 SUBJECT_SAMPLE_FACTORS 5 5B Sample Group:Low Insulin Study Date=12/21/07 SUBJECT_SAMPLE_FACTORS 6 6A Sample Group:Low Insulin Study Date=01/28/08 SUBJECT_SAMPLE_FACTORS 6 6B Sample Group:Low Insulin Study Date=01/28/08 SUBJECT_SAMPLE_FACTORS 7 7A Sample Group:Low Insulin Study Date=03/13/08 SUBJECT_SAMPLE_FACTORS 7 7B Sample Group:Low Insulin Study Date=03/13/08 SUBJECT_SAMPLE_FACTORS 8 8A Sample Group:Low Insulin Study Date=06/25/08 SUBJECT_SAMPLE_FACTORS 8 8B Sample Group:Low Insulin Study Date=06/25/08 SUBJECT_SAMPLE_FACTORS 9 9A Sample Group:Low Insulin Study Date=05/02/08 SUBJECT_SAMPLE_FACTORS 9 9B Sample Group:Low Insulin Study Date=05/02/08 #COLLECTION CO:COLLECTION_SUMMARY Percutaneous muscle biopsy specimens of the vastus lateralis (?300 mg) were CO:COLLECTION_SUMMARY at 180 and 480 minutes under local anesthesia (2% lidocaine). Muscle samples CO:COLLECTION_SUMMARY blotted, were dissected free of fat and connective tissue, and then were CO:COLLECTION_SUMMARY Biopsy specimens were collected from the opposite legs on a single visit. CO:COLLECTION_SUMMARY calorimetry was performed at 11:00 am for 20 minutes using a ventilated hood CO:COLLECTION_SUMMARY Medics). The final 10 minutes of steady-state oxygen consumption (Vo2) and CO:COLLECTION_SUMMARY dioxide production (Vco2) determined the respiratory exchange ratio (RER = CO:COLLECTION_SUMMARY Urine was collected throughout the clamp and analyzed for urea content at a CO:COLLECTION_SUMMARY laboratory. #TREATMENT TR:TREATMENT_SUMMARY Low Insulin | High Insulin | Low Ins+ Saline | Low Ins +EAA | High TR:TREATMENT_SUMMARY | High Insulin + EAA #SAMPLEPREP SP:SAMPLEPREP_SUMMARY MMP and muscle fractions were isolated from frozen samples using differential SP:SAMPLEPREP_SUMMARY (18, 19). Biopsy samples were homogenized with protease and phosphatase SP:SAMPLEPREP_SUMMARY (Halt; Thermo Fisher Scientific) and centrifuged to pellet myofibrillar (MYO) SP:SAMPLEPREP_SUMMARY The supernatant was centrifuged to pellet mitochondrial (MITO) proteins, and SP:SAMPLEPREP_SUMMARY final supernatant was deproteinated with cold ethanol (1:9, v/v) and then SP:SAMPLEPREP_SUMMARY to pellet sarcoplasmic (SARC) proteins. Aliquots from MMP, MYO, SARC, and MITO SP:SAMPLEPREP_SUMMARY acid hydrolyzed, and free amino acids were purified using cation exchange SP:SAMPLEPREP_SUMMARY and then were dried. Plasma phenylalanine enrichment was determined using SP:SAMPLEPREP_SUMMARY chromatography (GC) and mass spectrometry (MS) as described previously (19). SP:SAMPLEPREP_SUMMARY were derivatized to a heptafluorobutyryl isobutyl ester and identified with a SP:SAMPLEPREP_SUMMARY Quattro Micro triple quadrupole GC-MS system (Waters) operating under negative SP:SAMPLEPREP_SUMMARY chemical ionization using isobutane as the reactant gas. Selected ion SP:SAMPLEPREP_SUMMARY of m/z 399.2 and 403.2 M + 2 and M + 6 fragments of phenylalanine and the SP:SAMPLEPREP_SUMMARY respectively, was performed. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex API 5000 QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Dried amino acids were prepared as isobutyl ester derivatives, and the mass was MS:MS_COMMENTS by an API 5000 triple quadrupole mass spectrometer with a TurboIonSpray source MS:MS_COMMENTS Biosystems). MS:MS_COMMENTS Muscle protein enrichment was determined using HPLC and tandem mass MS:MS_COMMENTS (MS/MS) as described previously (19). HPLC-MS/MS has superior precision MS:MS_COMMENTS to those of isotope ratio mass spectrometry and GC-MS and accuracy comparable MS:MS_COMMENTS that of isotope ratio mass spectrometry (19). Dried amino acids were prepared MS:MS_COMMENTS isobutyl ester derivatives, and the mass was determined by an API 5000 triple MS:MS_COMMENTS mass spectrometer with a TurboIonSpray source (Applied Biosystems). Enrichment MS:MS_COMMENTS calculated as molar percent excess (MPE) representing tracer content (13C) over MS:MS_COMMENTS A calibration curve from 0 to 0.1 MPE was prepared using unlabeled MS:MS_COMMENTS (Sigma-Aldrich) and l-[ring-13C6]phenylalanine. Selected reaction monitoring MS:MS_COMMENTS performed for m/z 222.4 > 121.6 and 226.4 > 125.6 for the M + 2 and M + 6 MS:MS_COMMENTS of phenylalanine and l-[ring-13C6]phenylalanine, respectively. Muscle FSRs MS:MS_COMMENTS calculated as FSR (percentage per hour) = [(E480 ? E180)/(EP · t)]*60 · 100, MS:MS_COMMENTS protein enrichment of biopsy samples (E480 and E180), minutes between biopsies MS:MS_COMMENTS and enrichment (EP) of plasma precursor (10). The phenylalanine rate of MS:MS_COMMENTS (Phe Ra) was calculated as Phe Ra = [(FTracer/Ep) ? FEAA], with tracer infusion MS:MS_COMMENTS (FTracer), plasma enrichment (Ep), and phenylalanine appearance from EAA MS:MS_COMMENTS (FEAA). One participant showed decreased plasma enrichment (10.4%2.2%) during MS:MS_COMMENTS insulin + saline for unknown reason and was removed from the protein turnover MS:MS_COMMENTS The muscle tissue required for fractionation was not available for all MS:MS_COMMENTS and group sizes were 8 to 10 per fraction. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples 10C 10D 1C 1D 2C 2D 3C 3D 4C 4D 5C 5D 6C 6D 7C 7D 8C 8D 9C 9D 20C 20D 21C 21D 22C 22D 23C 23D 24C 24D 25C 25D 26C 26D 27C 27D 28C 28D 29A 29D 20A 20B 21A 21B 22A 22B 23A 23B 24A 24B 25A 25B 26A 26B 27A 27B 28A 28B 29B 29C 11C 11D 12C 12D 13C 13D 14C 14D 15C 15D 16C 16D 17C 17D 18C 18D 19C 19D 30C 30D 11A 11B 12A 12B 13A 13B 14A 14B 15A 15B 16A 16B 17A 17B 18A 18B 19A 19B 30A 30B 10A 10B 1A 1B 2A 2B 3A 3B 4A 4B 5A 5B 6A 6B 7A 7B 8A 8B 9A 9B Factors Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin + EAA Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:High Insulin+Saline Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins +EAA Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Ins+ Saline Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin Sample Group:Low Insulin 1-Methylhistidine 0.0000 0.0000 47.2929 29.0320 4.0370 2.2676 8.8792 4.3683 7.8247 4.0407 18.7500 7.2326 20.0647 10.9779 11.7638 5.9336 49.4524 18.7822 20.7286 9.9786 18.7851 16.1887 0.0000 13.0945 8.0034 5.3222 11.6717 9.6773 15.2346 11.6923 9.1263 4.5097 10.8963 0.0000 0.0000 7.5606 19.0704 10.6648 0.0000 14.3103 15.5059 8.1580 16.5564 13.1731 7.2325 3.9914 4.3250 2.2263 18.6153 8.1466 12.2627 8.1834 18.5124 10.7346 0.0000 0.0000 0.0000 0.0000 0.0000 14.7959 5.4809 2.7406 10.4355 5.9945 61.6930 80.4827 9.2613 7.3606 13.0056 9.5840 9.2549 6.4553 7.2690 4.9272 2.1745 0.9202 15.9744 13.6362 6.5582 5.1830 17.5726 4.3552 10.0086 7.9104 72.6238 79.2093 12.2495 6.4091 9.5295 9.1535 9.7950 7.1532 4.1423 2.6654 0.0000 0.0000 9.3292 5.9677 5.4069 0.0000 12.9040 12.5596 56.2903 37.0293 7.9352 3.4989 14.3573 9.2236 7.2938 3.2479 11.3415 9.1590 18.3009 11.8039 12.3137 7.4578 10.5742 8.5362 14.7617 6.8145 3-Methylhistidine 0.0000 0.0000 6.7442 4.5272 4.9799 4.2331 5.5260 4.0843 5.2486 3.7162 4.8669 2.8641 3.6839 2.8929 6.9063 4.6488 6.3693 3.8398 6.2971 5.3038 7.9769 7.8953 0.0000 6.1589 6.9227 5.3816 5.5210 4.3781 6.3713 4.9770 4.2527 3.6876 8.6772 0.0000 0.0000 4.3378 4.8472 3.1266 0.0000 3.9192 7.7949 5.6392 7.5194 5.8421 6.8075 4.3555 5.1601 3.5350 8.0157 4.8073 4.0160 2.9796 6.6934 5.5562 6.4238 0.0000 0.0000 0.0000 0.0000 4.8193 6.7140 4.4651 5.2748 4.1748 10.0124 13.8559 4.8947 4.1724 5.1477 4.7620 4.8710 4.4158 4.5339 3.3893 3.4718 1.6879 6.6182 5.8976 5.3640 3.2849 6.3865 3.1517 5.6038 4.5585 12.1658 12.1322 6.3239 4.6602 4.7531 4.7236 4.8824 0.0000 4.7553 3.8987 3.2021 3.6933 5.3652 4.9597 5.7856 4.6382 5.4714 5.8412 8.5725 5.1352 7.5037 4.9287 5.8677 4.4621 4.6859 3.6029 4.6240 3.1613 4.1617 3.5341 6.8772 5.5366 6.0218 4.5186 5.8465 3.7457 Alanine 299.8547 385.2816 266.1221 290.4619 226.5545 265.5815 229.2930 316.5119 233.5419 456.0523 205.1036 289.6133 311.5848 312.9199 237.7583 281.9088 297.6879 379.4424 309.3259 401.4587 236.7740 411.2258 209.1951 233.7259 320.1178 429.1329 220.5571 563.9297 285.5924 534.7492 186.4746 291.6530 249.5652 671.7950 274.9800 278.4976 269.2623 577.8587 201.5603 383.7269 163.7028 214.8210 207.1400 430.2982 232.8129 220.5661 251.0494 232.6193 279.2368 312.2150 221.6223 569.9892 190.3423 258.3468 283.0818 506.1384 188.5312 288.5775 237.6490 188.0408 236.3041 211.7325 240.0308 481.0969 305.0188 374.4024 211.1506 475.9907 190.4828 382.5184 328.4537 418.8831 303.8831 545.4155 333.4705 515.0757 232.4925 488.3549 310.9223 532.7009 265.5862 306.6668 227.2517 661.9233 239.7634 594.8233 285.1271 411.8618 151.5900 454.8708 291.5590 451.6938 329.8718 394.1671 328.5742 712.7785 250.9921 423.1716 249.6660 357.4131 187.7172 327.9635 262.4078 549.6357 171.1954 193.1296 236.1674 447.5074 205.3876 351.2427 269.6579 458.8431 415.1195 528.8773 243.1933 359.2557 275.4775 425.0807 329.2291 315.1816 allo-Isoleucine 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 alpha-Aminoadipic-acid 1.6859 1.0761 0.9442 0.4760 0.9530 0.8332 0.8495 0.5223 1.2979 1.1760 0.8941 0.5977 1.2219 0.9038 1.0026 0.7080 0.8754 0.6441 1.0198 0.9366 0.6632 2.2531 1.1038 0.7367 2.6714 4.5774 0.9949 2.6609 1.2360 6.9198 0.8128 0.6507 0.8911 5.4072 1.4599 0.6482 1.3517 4.4931 1.0016 4.8210 0.6481 0.6016 1.1124 3.1089 1.8450 0.7572 1.0268 0.6144 1.2424 0.9193 0.7894 2.2724 0.9217 0.6504 1.2137 4.6131 1.1233 0.8232 0.6938 0.9315 0.8821 0.7031 0.8290 0.9424 0.5575 0.5028 1.5604 9.1282 1.3020 1.5885 0.8365 0.9065 1.2625 7.2490 0.9404 2.5952 1.2092 4.6214 1.0154 3.2486 0.9138 3.9302 0.8613 4.8918 0.5825 2.9450 1.5369 1.2675 1.0126 5.6423 0.4008 2.4705 1.3677 1.1012 0.8593 0.9078 1.0379 0.9596 0.8163 0.5464 1.0619 0.9739 1.2950 0.9004 0.8771 0.5487 1.0399 0.8256 1.1571 0.9291 1.1681 0.9539 1.2203 1.2574 0.8744 0.9072 0.7300 0.9797 1.1072 0.8042 alpha-Amino-N-butyric-acid 9.6377 13.4817 15.2897 8.1861 14.7043 11.5478 33.2397 11.6435 22.3671 27.3034 33.7752 16.1121 39.4414 22.6432 28.5825 10.2024 9.2857 6.5703 6.8945 5.7536 14.1274 14.7344 16.4844 7.9534 25.2334 29.2153 27.9171 31.9973 23.8258 29.7089 10.7552 6.1009 17.3017 14.0438 11.6427 8.7680 7.3263 9.9283 9.8277 8.5876 11.9859 6.8970 14.0224 19.9603 28.1185 9.6494 24.7230 6.3626 21.1304 9.8732 10.4529 15.9227 17.3468 6.9101 12.0871 9.8130 8.5489 4.8008 5.4902 8.3568 26.0665 23.9748 12.3212 16.7735 8.5485 7.4972 36.2585 54.4444 11.0911 22.5828 27.9387 24.6991 30.0404 37.4570 12.7051 13.5653 8.8008 17.5639 19.2268 60.6195 12.5911 23.6630 13.8003 27.6667 10.4254 23.4891 28.3087 30.6388 27.6630 45.9887 11.6153 36.8978 18.4046 16.2667 13.0183 16.9242 11.0110 11.4435 11.9253 17.3645 7.9256 7.5206 12.7978 20.8889 14.4790 7.8128 34.5304 34.2677 23.7811 10.6765 27.6153 28.5780 42.3634 47.1512 27.2293 28.9924 9.5301 14.2247 5.3619 2.9115 Anserine 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 1.1064 0.7636 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.3508 0.1773 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 Arginine 84.2264 79.7216 46.8853 32.9271 35.5416 36.0292 33.2101 28.2769 40.9886 30.1165 72.9011 59.0217 88.9942 52.7408 80.3242 55.4241 86.1421 62.7779 52.9569 55.1396 29.3723 22.2792 42.0647 33.6685 96.1263 36.3298 65.4162 37.5201 79.3642 54.6380 51.9989 40.9292 69.4952 22.6278 84.0593 41.9334 85.7048 42.7216 57.1577 23.0062 30.1154 26.5447 41.3414 37.4351 72.0863 40.4324 55.9048 34.9707 73.2952 40.5325 59.5317 46.7546 63.6455 30.4274 68.1291 37.4195 48.3632 45.5903 33.7287 65.2252 33.6555 24.7914 43.7960 34.4238 42.7625 39.4556 61.7388 95.9924 84.2823 96.8592 78.8187 110.1776 66.3603 43.0615 58.9523 28.8669 65.4214 64.2385 33.2176 36.6201 31.3886 26.6092 36.3500 40.8948 42.0433 40.8533 97.6362 68.1068 100.7187 90.9504 63.4093 83.4310 64.2259 55.6456 69.1649 62.1782 56.6835 76.9270 41.7085 39.9068 51.9451 40.8261 38.2262 39.5828 41.2813 39.3857 33.7178 39.8777 46.9739 30.8297 77.4723 95.4099 74.7742 130.5333 77.5672 91.0433 85.6006 136.6583 66.9351 28.1013 Asparagine 112.8139 77.2016 70.6177 54.8989 67.4044 74.3748 61.9791 39.2338 77.0892 75.5990 75.6080 45.5826 57.2747 37.0454 62.1814 38.7085 74.3396 47.5220 73.3991 56.7245 101.5977 48.3363 65.7831 48.7616 71.5096 16.5957 67.3334 28.4617 68.0419 16.5410 53.6888 38.7391 107.7903 22.9199 58.2281 37.7817 82.3832 21.1783 77.1497 24.6486 79.4341 61.7060 66.0499 21.7558 75.1849 46.3148 67.8752 37.3011 66.5685 35.7256 51.8035 21.5132 68.9605 36.7913 69.0821 15.2126 73.8279 44.4526 40.6687 55.5988 108.5413 67.4203 86.1628 104.0102 125.8250 116.6788 49.5228 39.3777 55.2254 58.3572 65.9080 76.6779 69.5440 35.5008 71.1867 26.6433 68.9232 46.3232 80.4929 54.9055 129.3102 65.0984 82.1642 67.4954 122.8022 88.0931 65.3319 57.4677 48.0772 30.3523 81.9087 62.1475 68.9596 60.3575 74.5294 84.2415 71.0894 73.1947 60.8331 81.3965 67.3780 56.5637 57.1122 88.5342 77.9791 57.3714 65.9748 71.1535 71.9709 50.4781 64.4280 64.6522 54.6980 65.9162 65.9780 71.9050 58.8025 85.2795 75.9232 35.6648 Aspartic Acid 9.5230 2.7339 3.4302 3.4985 3.9571 2.4120 2.8647 1.8374 4.3072 2.8962 2.9112 1.4865 2.8535 1.5822 3.4446 2.1726 2.3561 1.5477 5.2076 2.1563 5.2331 5.7384 3.1129 1.8554 3.1849 1.9537 2.3999 1.2996 3.6011 2.1734 2.3098 1.1972 1.9542 1.4761 4.9373 0.8908 6.5337 1.3023 1.8440 4.1021 5.9388 3.3549 3.0824 1.4858 4.9408 2.3730 2.3496 1.4620 3.4338 2.0995 0.8310 0.7875 3.5047 2.3164 1.7735 1.0087 1.6148 0.4809 5.9130 1.5670 5.3437 2.6339 5.0464 3.4132 4.1353 2.5901 2.5302 2.6603 3.3625 2.0477 1.6826 1.7732 2.3273 1.7073 2.1242 0.7314 2.5738 2.1618 7.7277 3.3196 4.9454 3.5170 4.7095 2.8363 3.9415 2.3420 3.0755 2.8104 3.5375 1.9581 1.7852 1.8249 3.7951 2.0552 1.4333 2.1363 1.9728 6.3581 2.9382 2.8586 11.3277 15.7986 3.9497 3.5521 2.6278 1.7742 4.4331 3.0909 4.4166 2.1560 2.6045 1.9823 2.5009 3.1272 3.6110 3.1126 5.1478 1.5413 1.4135 3.2869 beta-Alanine 5.7969 6.4454 6.6264 4.9433 3.5311 3.1951 3.4776 2.9728 3.8173 2.7161 2.8358 3.0728 4.4795 2.4486 2.8221 2.0360 6.5342 5.9448 6.6186 5.1164 4.7643 3.9598 3.3614 3.5260 10.0223 8.7604 3.1231 4.1573 4.2767 5.0489 4.7519 4.7137 4.3127 7.1359 4.9651 3.7570 5.9953 6.1344 5.4161 6.5822 3.6051 4.2899 3.2251 4.2259 7.9531 4.9880 3.4972 2.7597 3.1700 3.0706 6.1490 5.1477 5.1766 5.3715 5.0230 5.0254 5.3211 5.4463 4.9260 5.2829 3.8937 2.9210 3.5418 3.4306 4.2945 4.0637 3.2336 3.8438 3.1564 2.8232 4.2225 4.1628 4.3793 5.3589 4.2083 3.1631 5.1041 6.6959 4.6220 4.9570 3.0276 3.1780 3.4616 3.9689 3.4537 3.8024 3.6002 2.9191 3.0268 3.7946 5.2105 4.6655 3.6235 3.7421 4.7592 4.9762 4.4169 5.0232 3.8726 3.5284 5.3688 6.6940 6.4915 4.9991 5.6456 5.0829 3.5192 3.3932 3.6778 3.5050 4.9824 4.6791 3.3305 3.0257 3.1434 2.7775 5.7425 5.6405 7.9064 4.8378 beta-Aminoisobutyric-acid 1.6378 1.0941 2.1472 2.0488 1.4095 1.7976 2.0754 0.8568 0.8168 0.6842 1.2419 0.8791 0.3239 0.2970 0.7004 0.5788 2.7790 2.1464 1.5583 1.2120 1.2316 1.2169 1.3677 1.0717 1.8604 2.3241 0.7795 1.0449 0.8584 1.3122 1.1040 0.8785 2.7335 1.7315 0.8359 0.5564 0.8826 1.0272 2.3902 2.5809 1.4630 0.8343 1.4926 1.2463 1.6908 1.4538 0.6209 0.5045 0.9248 0.6611 1.0010 0.9798 1.6305 0.4659 0.7642 0.9439 1.1307 0.6922 2.1338 2.6817 1.8791 2.3481 2.4036 2.0938 1.2278 1.9155 1.3409 1.1864 1.4100 1.8148 1.0951 1.5575 0.8093 0.7894 1.5604 0.6951 1.4868 2.0526 1.1483 1.4809 1.4941 2.3280 1.9776 1.9765 1.5013 1.9192 1.3064 0.9545 1.7299 2.1345 1.9363 1.7402 0.5066 0.5374 1.9166 1.4915 1.5518 1.4571 0.7773 1.5403 1.4131 1.1258 1.8796 2.3113 2.2918 1.5023 1.7769 1.4133 0.9673 0.5574 0.9837 0.9841 0.3052 0.3829 0.7428 0.7580 2.4526 2.6594 1.3731 0.7032 Carnosine 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 Citrulline 32.5615 220.1524 38.3992 24.8107 26.4741 28.8589 29.4650 16.0642 22.1333 31.0507 44.7729 29.5103 38.2781 19.2136 53.4634 26.5518 33.9630 39.4279 269.2740 30.6447 21.1952 20.9214 29.5706 16.2600 59.6251 50.3246 32.8916 28.7072 49.4059 40.1460 214.7722 18.6645 52.5215 45.4498 342.0672 21.2356 207.1308 96.9330 32.6624 209.6395 19.7646 11.2646 28.3815 20.2693 66.1497 26.8098 31.0216 15.6892 47.1153 24.4056 45.5963 30.8152 172.4103 76.6780 40.5840 33.4025 29.6510 29.3611 215.4281 36.9647 19.8848 20.8377 32.3904 28.7103 21.4967 22.6570 35.5719 44.4452 43.0159 39.1351 40.1422 44.0635 37.3120 37.7080 171.8151 86.4320 37.7314 34.8873 33.4696 26.2843 19.1836 24.7729 32.3550 36.5359 25.7747 28.1653 39.7370 34.3766 33.6284 47.7930 32.0714 39.3518 40.7596 27.4596 186.5390 31.9909 34.1149 134.8958 22.9695 31.5015 219.7270 24.7588 32.4066 40.6719 29.7913 16.9117 28.6206 27.8373 27.6592 18.7683 40.3285 42.6734 33.2609 34.7607 51.8779 34.7670 222.0330 129.6090 85.0977 133.6874 Cystathionine 1 1.3576 1.1921 0.9084 0.5517 0.6559 0.6760 0.5897 0.4629 0.6530 0.6278 0.5917 0.3754 0.4021 0.3091 0.5783 0.3723 1.2151 1.0513 1.1098 1.0399 0.5404 0.8323 0.6651 0.4742 0.6454 1.3527 0.4008 0.9567 0.5936 2.1124 1.1107 1.0295 1.1942 1.7967 1.1971 1.0493 1.1797 2.2561 1.1027 1.4306 0.5419 0.4459 0.6010 1.6182 0.7276 0.3659 0.4272 0.2848 0.4697 0.3337 1.0585 1.5101 1.1406 1.0405 1.2078 2.0211 1.1248 1.0229 1.0260 1.0926 0.6496 0.5823 0.5666 0.6280 0.6941 0.5291 0.4988 1.5787 0.4306 0.3680 0.4376 0.3185 0.5273 1.6661 1.2590 1.9344 1.1858 1.8605 0.8092 3.4732 0.7048 1.4127 0.5307 1.2759 0.6431 2.0406 0.6672 0.4260 0.4470 2.0902 0.3153 0.8392 0.5053 0.4120 1.1591 1.1143 1.1792 1.1031 0.7383 0.8693 1.1474 1.0439 0.9809 0.6882 0.5785 0.4759 0.6646 0.6546 0.5986 0.4359 0.5058 0.3556 0.5031 0.4780 0.5572 0.5029 1.2012 1.1066 1.2312 1.0227 Cystathionine 2 0.0000 0.0000 0.9947 0.5203 0.7681 0.7862 0.5309 0.5227 0.7657 0.7430 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.5243 0.9274 0.5236 0.5172 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.5639 0.0000 0.0000 0.0000 1.0338 0.0000 0.1894 0.5191 0.5188 0.5225 1.6322 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.2727 0.0000 0.0000 0.0000 0.7933 0.0000 0.0000 0.0000 0.0000 0.7629 0.7022 0.5199 0.6578 0.8027 0.6583 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.7057 0.0000 0.6528 0.9057 3.2960 0.8129 1.4468 0.5208 1.3241 0.5254 2.0129 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.8425 0.9602 0.0000 0.0000 1.0596 0.5255 0.6987 0.6069 0.5551 0.5347 0.7165 0.5186 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0323 0.0000 Cystine 78.9912 84.2005 21.6769 40.1850 43.9692 44.0640 49.6144 45.7801 45.2520 47.3706 47.8841 59.2165 69.0889 56.3728 101.4837 85.6355 92.5073 84.8747 84.1012 79.3786 46.1344 24.9278 57.9933 57.6266 49.2929 32.6493 64.9827 29.4592 68.9498 36.7237 73.7724 57.5122 104.6048 47.1492 108.7866 76.1602 87.7633 49.5206 72.1679 37.1094 38.4257 38.3476 44.9036 25.3381 74.2184 62.1675 50.3012 47.2016 77.5501 68.5398 64.6650 40.0601 97.0193 73.5274 100.4149 38.9171 68.5896 62.2731 79.5831 96.0577 30.4345 35.8808 57.8071 73.5983 43.9988 45.4845 65.2849 46.4704 61.4565 73.0698 92.9303 100.9928 70.1232 44.0788 57.3741 19.5928 99.2545 53.7368 23.6540 21.0444 18.0198 13.2595 55.4232 33.3467 37.7312 29.3118 78.7240 82.5535 54.5974 47.6013 61.1501 59.0853 79.8773 78.4364 74.9329 52.0634 102.7129 98.8437 24.5486 41.4990 83.8633 100.1761 31.4227 51.6933 26.3687 28.4961 58.7873 67.8220 41.0124 41.5693 53.0397 68.9863 60.0187 72.7772 102.4370 98.8802 85.6804 89.6461 84.9076 77.0700 Ethanolamine 6.2282 4.6648 8.5178 7.7971 7.4510 7.1143 8.1991 7.6915 7.5097 6.9829 5.1723 5.4585 5.5944 5.8256 4.6950 4.9254 4.1376 3.5086 5.3861 7.7983 10.9223 10.6238 6.9050 8.1810 5.5937 5.5603 5.0924 6.3591 5.2561 5.9643 2.9073 4.7171 7.5348 6.6452 3.8067 6.0395 2.6322 3.3470 6.3487 4.1848 7.6612 10.1651 6.4343 8.0261 4.9805 5.6392 5.1910 4.6047 5.7242 5.9460 2.9517 4.1182 3.9215 5.4622 6.0184 5.8594 3.8600 3.0025 4.3671 4.4425 6.0589 5.3301 8.1264 8.4840 6.3391 7.9006 5.9689 5.6397 4.9787 4.7502 6.6505 5.8503 6.7274 6.1041 4.5406 2.1355 3.9223 7.0797 7.7025 9.1821 6.6253 4.8830 8.2684 8.9941 7.6846 8.3748 7.2182 6.5692 4.4291 4.3806 9.5351 5.4070 6.7386 6.5258 4.5551 9.7574 5.0503 4.7005 8.8089 11.8714 2.9231 6.2107 7.8377 7.3625 8.7374 9.5185 8.3349 8.5735 8.5553 9.5518 6.3312 5.0397 5.7592 6.0870 4.9740 5.9550 3.3679 3.8907 6.5461 5.2914 gamma-Amino-N-butyric-acid 0.0000 0.0000 0.5529 0.5267 0.5296 0.5081 0.5085 0.4864 0.5614 0.5457 0.2379 0.2357 0.2383 0.2281 0.2608 0.2393 0.0000 2.5433 0.0000 0.0000 0.5199 0.5029 0.5061 0.5048 0.2641 0.2439 0.2585 0.2579 0.2517 0.2370 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 2.0744 0.0000 0.0000 0.5060 0.5121 0.5175 0.5136 0.2816 0.2622 0.2660 0.2282 0.2609 0.2384 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.4918 0.4825 0.4842 0.5042 0.5199 0.5696 0.2433 0.2249 0.2511 0.2720 0.2368 0.2490 0.2572 0.2456 0.0000 0.0000 0.0000 0.0000 0.5166 0.5340 0.4934 0.4680 0.4893 0.4979 0.5433 0.5603 0.2453 0.2449 0.2453 0.2526 0.2570 0.2345 0.2648 0.2496 0.0000 0.0000 0.0000 0.0000 0.5237 0.5514 0.0000 0.0000 0.5464 0.5542 0.5491 0.4957 0.5060 0.5108 0.5350 0.5314 0.2778 0.2776 0.2339 0.2299 0.2631 0.2821 0.0000 3.9315 0.0000 0.0000 Glutamic acid 50.4393 21.1800 31.5634 12.7191 30.0881 15.5732 23.9734 12.8931 28.6189 18.6478 23.8438 11.1953 33.5762 17.5796 27.4630 13.5123 37.4862 25.5557 7.5245 10.1613 12.2246 13.8632 30.9333 17.1866 22.9272 14.9247 15.6013 5.2506 34.8131 19.7731 26.9190 10.1448 40.5046 26.4630 45.3519 22.2747 49.2776 20.8463 36.0378 25.7555 18.1450 9.8406 27.1644 16.8850 27.7154 10.8701 10.3244 7.0124 30.9349 14.9379 21.2273 19.2446 45.8279 22.2961 39.0403 38.6921 33.4894 16.0614 21.5318 44.7937 30.9292 16.6102 24.7321 16.2485 16.8671 10.9784 32.8156 28.6078 25.5824 15.2583 6.0794 10.0416 13.4293 21.0122 31.8033 14.9967 59.3451 55.7095 28.3121 18.7154 22.1025 19.2689 21.4918 20.1331 15.4261 10.6225 16.5428 16.8139 24.0626 17.9756 4.3471 13.4017 18.5425 12.3534 31.1488 40.9175 58.3017 28.6664 19.1065 10.1296 55.2006 32.4619 22.9275 15.3445 30.2922 13.9681 27.9748 16.0639 28.1615 12.2662 19.4969 14.2946 21.9100 17.0062 25.1403 17.2854 28.8182 25.5916 12.9313 4.4193 Glutamine 308.7157 313.2285 434.3178 408.6568 411.7404 437.2945 388.6537 305.3708 400.5164 442.3960 277.5778 253.0154 224.6220 174.4598 241.1618 198.4704 371.1061 359.6703 301.8559 287.7415 350.9940 440.5140 381.8600 378.1689 288.2235 341.4212 199.4674 283.9951 299.3694 362.8259 291.0492 287.3525 393.6859 497.9962 354.6783 264.9623 300.2354 361.2767 248.5328 306.6246 297.0922 273.7428 381.9911 586.3563 215.4198 184.1882 180.5063 152.4407 287.6328 243.7527 302.0001 464.2851 341.4012 270.4930 303.3186 423.8652 236.3862 235.6181 197.1006 228.3872 453.3373 408.4142 405.5125 484.4521 398.2525 415.3297 234.3633 298.2094 212.2777 233.7398 259.9441 284.3995 317.8654 371.1372 357.6234 215.1138 277.3098 328.3408 421.4044 622.5618 332.1113 420.7796 404.8706 552.9890 347.1736 528.5812 279.8586 248.5645 208.0028 257.7203 236.2099 279.3220 275.0258 270.0024 390.7637 386.5244 287.9883 353.7238 319.9530 477.2548 270.2477 295.9160 401.4829 692.6701 495.3486 395.8580 379.3680 412.0461 403.2033 361.2041 280.3621 280.4062 196.5207 220.2186 239.8925 247.8880 362.2432 487.1477 309.4367 258.4834 Glycine 263.4757 360.8846 197.4351 230.2547 252.3152 328.1708 219.6542 238.3892 187.6521 240.9393 249.3690 320.9205 302.0285 287.5107 214.3502 205.7812 786.8664 872.7588 311.7519 360.6424 181.2534 160.6063 324.3022 369.5673 213.4226 157.0298 155.8607 150.5801 279.1677 192.5170 251.3329 294.9468 332.3906 253.7881 320.3379 308.8490 346.9321 229.8686 238.4096 219.8249 164.2929 186.1370 253.5812 223.3783 191.2911 203.9728 145.3185 143.0238 251.0203 220.5783 248.2272 222.6620 278.4719 276.2828 282.8291 245.5437 268.7088 333.0897 291.7797 229.1104 367.8912 345.4106 193.9117 292.5032 197.0646 266.4806 209.7528 212.4098 298.3712 441.6936 374.2225 532.3773 238.4130 199.6353 342.2581 168.0171 221.3941 177.0286 401.3345 290.1767 235.0327 140.2692 176.2385 195.7090 207.8383 221.9849 252.2337 288.4062 289.9651 293.7408 337.8455 301.9550 248.7812 297.8127 438.9614 726.9703 211.9142 360.2472 300.8987 385.4083 207.7240 354.2242 137.1050 234.7841 260.6268 275.4388 204.6970 303.5213 192.8674 225.3727 267.0169 342.6584 256.3883 316.1073 240.1865 335.5152 744.3922 834.2188 322.9173 273.7859 Histidine 93.2330 93.6598 109.4613 106.3509 99.9809 112.8827 87.9333 77.0799 150.6342 149.0266 99.3138 87.1616 69.8871 58.9374 85.0701 70.1756 96.8652 92.8652 104.6914 105.1328 156.7234 305.2095 59.8584 91.4762 174.9162 170.4323 102.7570 187.3641 100.4725 198.0001 97.3047 91.6024 106.6852 213.8618 117.5993 84.4377 100.3535 235.2708 107.0158 245.0338 134.4465 124.6323 90.8526 253.2468 88.5002 70.7078 98.9194 80.8703 114.9150 91.8255 102.4348 203.2984 88.3523 81.8858 111.9795 210.1556 91.9968 84.7981 100.1326 100.2877 121.6824 99.1665 114.8495 116.2828 74.5571 119.8455 62.4657 166.2997 70.4140 71.7139 86.0031 84.6806 92.9119 227.3155 80.9762 114.7313 97.9823 205.5333 107.0179 255.7198 131.0459 254.2927 105.7056 286.1746 124.4532 290.1836 83.8335 74.8618 67.0418 153.0898 67.4732 167.4196 95.7976 99.8961 89.8013 105.2895 96.7074 109.5092 120.0328 143.5296 77.1693 101.3396 102.0994 130.0097 134.0861 115.0670 94.3228 87.6374 157.7440 143.2830 95.4124 94.2962 77.2017 79.4556 95.2913 85.6508 89.6010 98.8726 97.9202 81.3840 Homocystine 0.2676 0.2037 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.2025 0.1441 0.2742 0.2583 0.0000 0.0000 0.0000 0.0000 0.1835 0.2034 0.0000 0.1292 0.1896 0.3354 0.2187 0.1565 0.2573 0.9262 0.2668 0.1763 0.2256 0.7982 0.2178 1.7864 0.0000 0.0000 0.0000 0.0000 0.0384 0.0000 0.0000 0.0000 0.0034 0.0000 0.2654 0.7034 0.2294 0.1546 0.2897 4.6395 0.2138 0.1549 0.1577 0.2352 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0044 0.3277 0.0000 0.0000 0.0000 0.0000 0.0094 0.3558 0.2273 0.5716 0.2445 0.7334 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0404 0.0000 0.0000 0.2424 0.0000 0.4857 0.0161 0.0000 0.2150 0.1968 0.2271 0.2153 0.0000 0.0000 0.2546 0.2108 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0042 0.0000 0.0000 0.0000 0.0000 0.0000 0.1701 0.2188 1.3645 0.2258 Hydroxylysine 1 0.0786 0.0703 0.3807 0.3594 0.3705 0.3702 0.3935 0.3517 0.3339 0.3413 0.2390 0.2310 0.2101 0.1744 0.2091 0.1703 0.0391 0.0270 0.0397 0.0427 0.3479 0.3645 0.3735 0.3462 0.1693 0.1684 0.2031 0.2331 0.2669 0.2441 0.0420 0.0236 0.1005 0.0758 0.0542 0.0081 0.0376 0.0553 0.0380 0.0690 0.3468 0.3371 0.3720 0.3825 0.1926 0.1485 0.1928 0.1513 0.1834 0.1622 0.0387 0.0672 0.0584 0.0115 0.0195 0.0742 0.0465 0.0131 0.0284 0.0578 0.3945 0.3908 0.3868 0.6393 0.3715 0.4018 0.1933 0.2349 0.2059 0.2073 0.1951 0.2109 0.1838 0.2111 0.0738 0.0289 0.0631 0.1037 0.3653 0.3955 0.3789 0.4498 0.3582 0.4131 0.3734 0.4358 0.1725 0.1854 0.1917 0.2474 0.1654 0.2549 0.1844 0.1660 0.0747 0.0679 0.0680 0.0674 0.3409 0.7536 0.0795 0.0551 0.3678 0.3836 0.3609 0.3401 0.4207 0.3917 0.3320 0.3103 0.2072 0.2097 0.2030 0.2033 0.1903 0.2083 0.0399 0.0466 0.3027 0.0348 Hydroxylysine 2 0.5400 0.4677 0.9283 0.8505 0.8758 0.9439 0.9666 0.8178 0.5978 0.7006 0.9926 1.0041 0.5391 0.4640 0.6454 0.5794 0.4299 0.3994 0.3752 0.4065 0.7321 0.8729 0.7504 0.7091 0.5053 0.5300 0.6767 0.8407 0.7969 0.9726 0.3175 0.3214 0.3192 0.4466 0.4040 0.2000 0.2814 0.4507 0.3829 0.5602 0.7343 0.7019 0.7300 0.8640 0.5852 0.4126 0.6310 0.4902 0.6767 0.6404 0.2908 0.4332 0.2832 0.2151 0.3110 0.4769 0.2670 0.2475 0.3125 0.3775 1.0248 1.0650 1.0929 1.6471 0.7769 0.8711 0.4970 0.7376 0.6072 0.7345 0.6584 0.8659 0.5052 0.6331 0.2899 0.2008 0.3763 0.6323 0.7757 0.9907 0.8885 1.1607 1.0610 1.5598 0.8455 1.2657 0.4431 0.5604 0.5544 0.8358 0.5798 0.9406 0.5394 0.5145 0.2962 0.3829 0.4202 0.4942 0.6910 0.8028 0.4133 0.4205 0.8784 1.0843 0.7845 0.7460 0.9604 1.0509 0.5743 0.5422 0.9340 0.9509 0.5104 0.6070 0.5724 0.7849 0.4297 0.5891 0.6986 0.3191 Hydroxyproline 10.7882 7.3312 14.7728 12.0515 10.0697 10.6994 12.0800 10.4067 6.5051 5.8795 7.3697 4.4101 4.1326 3.5180 5.6668 4.6441 13.1321 8.2814 10.3904 9.5680 11.8509 12.9063 0.1837 6.2280 6.4255 5.8384 5.1062 5.3730 5.8944 5.8489 7.1481 7.0917 11.7452 8.6272 6.1916 5.8302 9.2107 8.2698 12.7148 10.4028 10.8148 9.2641 6.4098 8.0241 6.4570 5.1370 4.7710 3.6176 6.9006 4.8545 8.3694 9.8525 9.0572 6.9346 8.4754 7.5456 9.3207 6.0570 6.2545 9.6374 12.0222 8.9115 13.2878 14.4238 14.1221 17.1371 4.5766 4.7075 4.3555 4.5062 5.3563 6.1748 5.5009 4.8644 4.6433 2.8986 6.5415 9.9467 8.3254 9.6309 9.7724 8.4278 12.8576 17.6029 16.6909 20.9572 5.9094 4.7036 4.1902 5.0908 8.5980 5.9436 5.8923 5.3411 4.3429 6.1111 7.1684 6.2107 11.5211 10.9975 7.2754 9.2078 13.7884 12.7945 12.9332 10.9568 11.5926 12.9399 7.3048 6.6417 0.6361 5.7772 4.7761 4.3106 5.7146 5.4162 10.5700 9.9498 9.3573 6.7388 Isoleucine 133.6738 45.8083 105.9345 39.4349 62.8163 49.4931 87.0729 16.0027 107.9844 67.8429 90.8093 28.5692 75.4943 19.2792 74.7959 17.2091 73.8701 27.9469 79.8600 39.9780 68.4499 173.7835 66.0473 15.2253 242.2491 184.7408 83.3970 162.7235 89.0820 226.5695 80.4340 22.3734 125.6047 264.9220 121.2389 19.3211 96.3933 195.8761 108.0945 233.0898 57.8921 16.0957 63.7843 191.5844 112.5423 16.7055 68.7783 13.9643 81.2378 21.0067 63.5360 192.3656 111.3795 11.0842 109.5355 321.8275 68.4297 16.0047 16.6972 88.4021 106.0803 62.0399 82.2408 49.9940 48.7965 34.3574 90.7893 337.5715 64.5310 41.7499 61.9709 53.5034 95.5271 310.2277 106.3114 168.9605 99.0689 392.4787 80.3194 281.3185 84.6206 327.4983 68.3631 300.0209 46.3915 243.5424 114.0488 61.1015 54.2718 233.1425 67.5384 222.2734 89.5122 40.7496 91.9610 51.3653 87.7405 51.2582 62.2721 54.3014 95.6386 20.3224 118.6427 105.7505 68.9890 14.5684 92.5441 49.7797 96.7381 20.4105 94.7233 56.5200 61.5676 51.8086 69.0917 39.7949 60.3536 83.2301 72.8857 11.0037 Leucine 162.8621 99.4099 164.9949 90.1571 131.6611 127.8217 163.4744 56.0387 167.4174 136.7188 148.8214 64.4131 137.9465 61.8082 139.4514 48.8914 91.0989 47.0923 124.6873 77.8526 125.6467 286.4212 120.2349 54.5560 387.3490 310.8133 141.8050 267.0488 165.8177 373.3292 103.9201 46.2908 188.7184 316.9236 167.6525 52.9481 137.7678 277.3807 161.9343 350.3508 108.4681 54.0439 120.4710 322.1170 193.3266 54.9837 119.9545 45.1787 156.1993 68.3227 84.8180 226.0865 158.6891 51.2008 157.3780 383.7746 113.6195 45.0702 62.4812 141.7108 161.3327 114.2196 144.7600 112.6868 75.8662 69.8435 178.5739 563.3987 120.3141 98.0375 114.1540 115.0559 170.1747 522.2497 159.1474 215.4920 158.9470 561.8893 124.7746 450.6448 129.6730 528.8455 132.0522 502.1863 78.2218 401.8924 211.4348 139.5585 98.5909 387.3518 124.6285 378.1762 168.0130 113.5240 132.7853 96.8217 138.8024 119.7131 113.9700 133.5769 143.9304 81.5528 190.5534 204.5837 144.4170 67.1322 169.5180 117.9647 165.6826 60.7722 156.6931 114.4229 128.5988 124.8557 132.2470 98.7208 75.0340 117.1581 118.3198 40.9122 Lysine 339.5560 223.7828 217.4691 200.1702 296.2927 248.6711 236.4373 137.7701 257.0731 289.8394 262.9761 238.6442 346.8804 294.3554 220.0683 160.6625 167.4224 171.7285 172.3229 123.0797 251.1491 577.3312 241.9330 201.6023 520.6150 493.8442 262.4455 704.1866 247.8400 660.6844 106.0113 95.2404 220.0326 1529.3855 288.5289 137.3774 196.3007 1279.9384 159.6697 1222.4793 199.0618 215.6042 223.9622 650.9905 188.0655 143.8546 243.5928 145.2999 189.4054 141.1489 98.6237 973.8264 240.6218 113.1694 212.2313 1564.2400 167.7024 137.2796 96.4502 150.2533 240.3102 218.8650 141.3048 214.4972 248.6113 222.6616 304.1958 888.4544 297.4072 317.0870 247.0346 285.4466 259.0431 768.1188 133.1683 365.9284 154.2480 1603.8399 243.7809 875.5568 213.8939 517.1606 137.9541 611.3000 213.9379 864.0454 220.4046 261.3323 246.1639 651.7717 291.0832 651.4248 265.6885 236.5673 152.3586 169.3366 217.6579 722.2340 176.7040 273.0964 164.0084 127.2004 194.2875 345.9852 242.4759 201.5305 239.0725 261.4840 185.3915 139.6721 301.0113 269.4822 296.2178 425.8083 216.1991 370.0155 154.7223 269.2692 214.9086 72.1035 Methionine 34.4995 25.6437 28.0001 19.8530 24.8135 28.5168 25.7345 11.2661 26.2440 29.3683 34.3775 16.5626 28.0930 11.5739 29.1466 11.0813 27.0260 15.0002 34.0405 22.1920 28.0175 686.5381 20.9181 9.1497 362.5843 743.9722 29.4858 614.3500 36.1996 726.6021 23.1588 11.2624 31.3439 752.0826 31.1251 8.0097 32.2146 663.6292 25.9583 743.5385 22.5334 12.8683 20.4391 565.2458 39.5940 9.1317 28.4610 10.1570 34.7704 10.9944 21.3524 631.1703 23.8839 5.8637 27.5264 637.8377 23.1080 10.3322 9.9426 23.3365 33.7435 23.0941 28.9790 28.3593 24.1297 18.1053 26.9119 807.1774 28.5426 28.8409 34.3003 36.8926 38.9454 856.5073 31.8179 410.1566 26.0672 786.2301 27.8009 663.1625 27.5756 967.1060 27.4585 1064.1691 20.5696 865.0723 42.4639 36.4437 20.4388 632.4487 28.1400 515.9293 38.9927 26.7440 30.0041 25.9747 23.5546 23.8070 19.6392 28.7083 24.6858 10.0684 24.8023 38.5031 22.4458 9.5256 29.5157 31.5886 24.7781 10.9942 38.2020 34.7855 31.5911 34.0878 30.5986 25.3143 27.0245 34.8763 34.1283 7.7379 Ornithine 48.0338 22.0946 88.2521 47.9566 81.5734 31.4800 56.4084 20.2697 52.1038 46.7691 83.3916 47.0178 124.1658 75.3878 64.3823 32.1868 21.2586 11.4200 20.8342 15.9085 25.8374 8.4302 98.8381 55.8836 41.7523 14.9390 63.7541 32.6879 68.5611 31.6732 22.7319 12.1655 44.8809 11.1194 37.4794 17.8334 35.5701 11.2378 32.7817 9.7659 24.2965 19.4817 73.3572 46.6735 71.2731 30.7750 60.2157 19.9668 51.4993 33.2250 17.5770 11.0566 43.5342 14.9989 34.3283 16.5258 15.8872 8.8738 12.8477 22.0306 60.8921 38.0841 37.6856 46.0165 50.6328 27.4494 89.7075 46.6324 93.4330 65.5917 67.4224 54.3968 90.3889 29.5444 36.9927 6.5394 26.7177 12.9707 89.7946 52.4118 38.0247 15.9865 33.0109 27.4680 43.7732 23.7569 64.5566 63.8899 73.4612 40.5447 22.1207 33.7042 84.9701 44.0223 38.5320 43.3613 31.9132 30.0542 51.9250 51.6846 30.3513 22.3750 68.2177 78.9617 41.7498 19.9499 80.0253 49.1316 39.1988 16.0949 87.1899 49.3509 98.6505 105.6883 62.9907 85.3551 15.1022 17.1921 19.0882 9.7296 Phenylalanine 82.1842 50.7062 62.4486 47.6374 50.0670 44.4171 55.7509 34.6067 62.8857 46.6345 60.4879 39.7535 51.0747 36.5775 61.9702 41.4333 48.2443 36.4694 53.1160 41.9261 65.2038 272.3942 49.2962 37.7954 228.7697 298.4344 51.2199 238.2464 59.5585 330.3486 42.3605 34.9108 72.9464 336.1041 56.2146 36.9911 56.6751 374.4393 67.6028 345.2555 50.7681 45.6709 47.3243 263.0411 62.1794 38.5736 48.7965 34.6802 58.7372 39.1362 34.4398 234.6251 50.3189 35.7601 51.6802 312.9131 49.4446 38.4997 39.5453 56.6470 63.5040 44.1469 57.1193 43.8336 50.7145 36.4352 66.0728 418.8770 54.1673 49.7359 61.6835 57.6951 69.5340 358.8052 49.6182 192.0701 55.4201 322.7481 47.3682 296.9068 53.0601 286.9696 53.5696 306.8174 43.2279 321.6852 85.4582 58.3301 43.3319 285.7472 66.8546 278.4367 73.7344 50.3034 45.1980 44.4222 53.7714 45.7098 43.8202 47.8134 49.2786 49.0598 64.4350 65.2507 50.1266 35.8287 64.6850 50.0057 58.6183 39.7173 60.1599 45.5206 56.8525 51.3833 61.4964 52.6002 44.4795 46.2906 52.1120 33.0277 Phosphoethanolamine 1.4936 0.6925 1.0019 0.9166 1.0206 0.9831 1.0729 1.0028 1.4104 1.0935 1.0602 0.7973 0.7902 0.7318 0.8232 0.7763 0.2323 0.4733 1.6126 1.0074 1.4232 1.1720 1.0468 1.0674 0.6143 0.6488 0.8333 0.6546 0.8155 0.6348 0.5391 0.5975 0.7497 0.6681 1.4371 0.9846 1.1149 0.4584 1.2289 0.6998 1.2865 1.2777 1.0465 1.0009 0.6587 0.6205 0.8412 0.6558 0.8087 0.7974 0.7880 0.5753 0.8233 0.5915 1.5574 0.7951 1.0279 0.7377 0.4558 0.9240 1.3775 1.1014 0.8735 1.4858 1.6121 1.4958 0.8822 0.6222 0.7337 0.5567 0.7532 0.5265 0.9382 0.6243 0.6540 0.1215 0.7438 0.8132 1.1020 1.0045 1.3193 1.0054 0.8638 0.0000 1.7491 1.5665 0.9880 0.8249 0.6968 0.5256 0.6587 0.5258 1.0109 0.7993 0.5399 0.1249 0.8688 0.6618 1.1005 1.0978 0.7639 0.8850 0.9689 0.9145 1.0648 1.1063 1.1505 1.0604 1.2311 1.1557 1.4235 0.8421 0.5155 0.5481 0.9828 1.1308 0.1659 0.0643 3.1727 0.5313 Proline 202.5259 152.1420 207.7086 147.3107 167.6404 143.3917 195.9627 128.8497 189.3691 175.3973 206.4400 127.3710 213.2856 128.6060 253.1675 140.5022 205.7347 154.3153 162.3398 119.1012 134.7561 80.3745 163.9147 86.5095 162.3764 107.5247 140.5874 83.1963 242.8683 137.7476 231.2221 149.5745 232.5018 122.5541 194.3306 106.9270 203.6362 105.0926 162.1712 93.4967 115.2579 71.4702 152.6582 79.8573 186.1004 107.6949 126.8363 70.9423 227.5265 140.1655 217.1340 140.1691 179.8543 106.4411 179.8894 105.9880 146.8897 94.5499 100.6501 133.1875 166.9553 105.7345 204.1623 198.6335 155.8442 106.9586 172.8981 152.5065 120.8711 114.7492 168.7403 155.0274 238.4370 164.0794 292.2097 106.1722 163.8764 114.8964 177.5309 131.3303 131.1808 85.9542 160.8854 139.0637 107.2211 70.9680 189.5450 160.7550 102.9582 86.3744 155.9811 121.6341 236.6083 185.6098 294.6169 239.4952 152.2732 139.8669 135.3545 142.3967 155.4966 134.9433 182.3022 230.0112 155.4578 97.0911 199.9043 186.9724 173.3285 123.8071 217.2111 183.0607 212.4963 204.6910 253.1507 190.0210 179.9963 199.6429 160.4773 91.0036 Sarcosine 2.4616 18.4028 1.1991 0.8453 1.5509 1.5179 1.1622 0.6920 1.0970 0.8853 1.8363 1.1590 1.1450 0.7548 1.9510 1.0883 1.8377 2.7152 22.9934 1.4710 0.6494 2.8556 1.0659 0.5171 4.0897 4.6687 1.2825 3.2942 2.8627 6.8541 10.0054 0.7444 3.1241 6.6269 20.7086 1.1581 18.0522 22.5509 2.7207 70.4730 0.4715 0.1867 0.8867 5.7376 2.5845 1.5238 1.1759 0.6555 2.5552 1.6957 1.6157 3.8710 9.1425 4.9949 1.9524 6.6196 1.3658 1.4514 27.4851 2.6571 1.3159 0.8240 1.1265 1.0610 0.4669 0.2955 2.3055 5.7544 1.0253 0.8880 2.5243 2.0995 1.9223 5.0827 8.2290 20.8681 1.5716 5.0954 1.1650 7.3709 0.5401 2.4756 1.0828 4.6696 0.4909 4.0459 2.8585 2.3055 0.7855 4.2290 1.9710 8.6669 1.8034 1.3242 9.7813 1.5503 1.4442 7.9526 0.8197 0.7163 18.3416 1.7573 1.1506 1.1217 1.3326 0.8782 1.3003 1.1091 1.2497 0.8186 1.7589 1.3207 1.1999 0.9858 1.9079 1.3477 17.2500 7.3442 5.6652 9.8089 Serine 114.2469 89.0741 143.4613 133.9903 98.2191 105.7718 123.0119 84.3859 120.2730 121.8753 133.7597 98.8483 131.2260 84.4925 103.1325 76.7421 136.9573 111.5756 94.5445 91.1238 101.2039 111.1036 107.6016 82.9138 99.9619 56.4568 110.0257 75.3424 117.6360 71.7728 92.6043 65.7547 123.2551 41.5697 118.8445 63.2962 136.2303 71.8818 90.7432 52.4221 103.6636 80.1545 95.5553 74.7698 113.3703 65.6479 98.1848 63.1683 96.2580 59.0848 89.7670 68.1762 106.4005 51.3368 102.7883 60.5351 109.1103 76.9371 62.1980 82.5617 171.4879 114.5569 103.1211 122.7468 116.4952 102.8429 109.4607 97.1469 115.7687 117.7486 114.8018 144.1189 98.2980 73.1676 94.6750 32.8569 98.3730 87.9372 180.7659 133.8657 118.8641 84.4469 99.8311 92.2572 106.3059 113.6251 110.9351 114.0374 124.4521 95.4910 100.1537 106.8897 91.9908 87.6236 109.4465 106.1504 92.1413 106.8805 98.7722 147.4374 97.3667 143.8866 123.2892 192.2128 107.8112 78.0864 128.5523 143.7895 127.1031 80.3035 117.6301 127.5911 113.6305 138.6167 104.8410 130.1516 136.2708 193.7701 100.6614 56.7054 Taurine 54.2220 43.8542 79.8894 133.9338 55.8774 82.4030 67.1704 40.2377 70.5103 68.0849 45.5660 38.5364 40.1239 31.7408 49.1459 44.2129 58.8350 57.2986 50.3531 45.0837 96.7060 101.8758 73.2290 52.1810 54.2329 57.0412 43.1993 42.2788 35.7067 33.5187 51.5116 43.8988 54.7112 53.6156 51.9673 33.6456 57.3752 45.6575 39.6791 53.5236 71.7822 49.1011 59.8134 54.0131 57.0106 53.6628 37.4221 36.3397 41.3048 35.8122 50.2508 43.7399 47.1845 42.6424 47.2874 36.4257 46.8594 41.5852 33.1044 48.0148 128.6594 75.0939 94.3470 87.9188 79.0253 46.7514 48.6201 52.4405 42.0890 41.7542 51.6398 45.6323 44.3409 40.0372 48.6482 24.9800 77.6638 60.4505 75.2433 99.9119 88.3621 94.3568 91.3305 91.4114 45.7903 55.6946 60.7094 50.1142 36.8811 46.1714 53.7032 52.4425 51.8283 46.5284 36.7278 42.9898 62.3581 55.3952 50.8679 96.2380 42.4807 45.4248 69.4147 189.0938 66.9734 35.6465 78.1543 92.0088 62.8824 32.9621 54.8767 40.4355 32.3811 35.3615 59.7687 52.5009 49.9678 52.7996 66.0063 43.9501 Threonine 161.8132 139.6460 143.1773 106.0724 100.1157 113.6343 126.2779 73.0369 141.0415 144.3603 163.9054 109.0713 120.0432 72.9972 109.0576 58.6006 148.1770 110.3715 134.1146 105.3329 171.6723 426.6934 108.6981 62.7401 249.6777 314.4524 182.4576 419.3831 148.3661 381.8944 108.6520 59.5126 149.7275 428.9492 124.0860 64.8450 131.2482 391.3091 109.4034 342.5119 171.3426 115.6137 113.4229 373.0270 127.8655 63.3311 185.5233 103.4914 125.0096 66.4827 127.6889 354.2261 115.1688 58.6576 109.1920 367.2757 118.0318 71.6491 72.1827 101.0604 192.2574 136.0707 126.1052 147.9058 202.0054 184.2927 85.9000 388.7923 151.1924 152.8718 135.9025 158.9154 127.1697 421.1610 156.1455 285.5671 100.4515 444.0870 165.5744 477.4526 233.8523 557.3670 104.0931 486.2311 182.9793 749.8330 107.6761 101.2405 117.6112 462.3849 108.8708 371.9868 120.1746 104.7059 153.8976 146.1056 98.4026 111.2206 84.1573 111.7778 105.9803 93.8789 142.0773 188.6415 89.5402 58.9362 126.9113 148.1414 111.9562 66.1253 180.0971 180.9148 115.9675 134.2130 111.3507 114.7481 148.5445 201.2725 140.0885 75.6748 Tryptophan 59.8037 35.5206 65.4267 36.1210 52.7513 38.2386 54.5934 28.0337 51.8932 28.6018 84.2882 40.2664 69.5515 32.8469 56.6904 26.6886 54.3775 31.8677 77.8350 40.1854 64.1911 93.8418 58.6512 29.6185 112.2981 106.1806 62.7870 97.4316 68.5691 102.9887 56.3240 27.7508 78.1298 111.5785 57.8626 25.8419 71.9736 117.3721 80.7977 151.0205 57.0455 40.3820 52.5631 92.9824 72.1383 26.7941 59.1577 28.4500 78.5654 30.2025 49.5025 99.1106 53.0614 26.7777 56.2798 100.5931 58.9986 34.7491 33.6686 72.4355 68.9091 45.8829 61.1941 36.2610 58.6792 40.9652 67.2346 115.4865 59.7976 47.2491 55.1503 41.8388 71.9995 142.8879 74.5928 85.1054 74.9811 131.0192 52.1920 98.3295 66.4743 104.5772 58.3786 117.1024 58.2497 114.1396 77.6130 41.3587 41.4473 120.9138 51.9100 108.1147 83.2441 40.3574 53.1261 42.9450 59.2762 42.6491 57.5877 37.7167 56.7256 34.4494 76.2203 42.2447 59.6230 29.6342 60.9758 42.5968 45.8590 22.3072 91.2335 49.2838 76.2255 44.9064 60.6584 38.0078 53.5003 33.2416 75.2030 30.7951 Tyrosine 70.3129 50.3782 78.8608 49.9735 48.3614 51.9320 59.2224 43.1955 63.4183 55.8085 77.3496 61.7936 85.0365 53.3894 59.1889 35.4605 49.2403 29.7966 76.0609 54.0706 55.5312 80.2679 66.2252 35.5342 65.7673 113.1419 45.3775 71.7362 78.1887 135.3001 53.3613 37.4820 57.6122 149.5061 75.5140 35.7202 70.3727 141.9424 79.4368 163.3516 47.9077 30.0769 55.2821 82.7119 57.5252 29.0454 39.9521 18.1977 62.6464 32.9930 45.7591 88.7746 48.4207 27.8042 69.9242 140.2081 54.4823 34.1722 55.2372 70.5624 73.2420 39.1180 50.6355 60.0943 39.0717 26.8952 71.0041 95.0910 65.4361 58.5957 76.2803 69.2343 71.9494 95.1344 62.8686 53.5355 54.5883 113.8196 62.9509 90.2239 41.4986 54.5741 56.4062 140.1748 33.0441 82.6766 82.6591 61.1647 47.5050 100.7530 76.7441 93.5825 73.1491 57.6476 57.4366 50.3104 52.1911 58.8242 50.7299 55.0946 50.8413 49.5635 92.2943 103.4786 43.6274 37.1821 73.2063 74.1678 53.7761 40.5252 79.8087 80.8696 92.1036 86.1705 62.1383 58.9936 49.5319 49.6650 70.4665 34.6358 Valine 347.1732 216.5445 346.3283 200.6000 225.6599 199.9230 257.0028 114.7812 294.4031 237.1038 292.9948 153.1485 264.7459 143.6255 272.9200 120.4916 194.6066 100.7149 257.5418 158.2389 214.2989 482.4277 246.2435 127.6124 583.2190 558.7978 269.4309 491.1309 322.2088 625.7736 225.4470 99.7072 353.0291 596.8665 344.8907 146.3282 283.2522 584.4180 316.9523 690.9904 206.5850 106.9459 245.9309 551.2496 349.3389 142.5409 255.5079 109.1456 294.7380 156.6719 207.9655 439.2393 289.8256 130.1696 323.8757 717.0036 227.2758 103.3738 171.2863 281.1003 271.0790 204.9436 234.6524 184.0205 188.5520 135.1047 308.0558 805.9557 235.3750 184.6797 239.5412 212.3911 319.0871 814.6324 300.8271 367.8563 319.0017 928.2012 244.3703 703.9573 256.8548 840.9989 216.8191 732.6385 173.7029 712.3858 346.6306 239.6470 189.4600 655.7145 242.4180 619.9128 298.3795 208.6237 258.8581 174.9483 304.5467 231.1474 230.5156 212.7757 300.2963 207.9323 388.2600 327.9886 233.8137 135.9938 284.2943 196.4257 296.9085 139.9930 293.3013 212.3524 251.6495 220.3006 259.9569 184.8064 181.6754 214.0054 245.9480 106.0549 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type 1-Methylhistidine MAYO_ID 3-Methylhistidine MAYO_ID Alanine MAYO_ID allo-Isoleucine MAYO_ID alpha-Aminoadipic-acid MAYO_ID alpha-Amino-N-butyric-acid MAYO_ID Anserine MAYO_ID Arginine MAYO_ID Asparagine MAYO_ID Aspartic Acid MAYO_ID beta-Alanine MAYO_ID beta-Aminoisobutyric-acid MAYO_ID Carnosine MAYO_ID Citrulline MAYO_ID Cystathionine 1 MAYO_ID Cystathionine 2 MAYO_ID Cystine MAYO_ID Ethanolamine MAYO_ID gamma-Amino-N-butyric-acid MAYO_ID Glutamic acid MAYO_ID Glutamine MAYO_ID Glycine MAYO_ID Histidine MAYO_ID Homocystine MAYO_ID Hydroxylysine 1 MAYO_ID Hydroxylysine 2 MAYO_ID Hydroxyproline MAYO_ID Isoleucine MAYO_ID Leucine MAYO_ID Lysine MAYO_ID Methionine MAYO_ID Ornithine MAYO_ID Phenylalanine MAYO_ID Phosphoethanolamine MAYO_ID Proline MAYO_ID Sarcosine MAYO_ID Serine MAYO_ID Taurine MAYO_ID Threonine MAYO_ID Tryptophan MAYO_ID Tyrosine MAYO_ID Valine MAYO_ID METABOLITES_END #END