#METABOLOMICS WORKBENCH araskind_20150608_9571701_mwtab.txt DATATRACK_ID:293 STUDY_ID:ST000192 ANALYSIS_ID:AN000294 PROJECT_ID:PR000163 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE Time Restricted Microbiome Studies PR:PROJECT_SUMMARY Study Time Restricted Microbiomes PR:INSTITUTE University of California PR:DEPARTMENT Gastroenterology/Internal Medicine PR:LABORATORY Zarrinpar Lab PR:LAST_NAME Zarrinpar PR:FIRST_NAME Amir PR:ADDRESS San Diego, CA PR:EMAIL azarrinp@ucsd.edu PR:PHONE 858-442-1359 #STUDY ST:STUDY_TITLE TRF Microbiome Study NA/FA/FT/CDKO 4-14 ST:STUDY_TYPE This is a time restricted feeding study (see M Hatori, et al. Cell Metabolism ST:STUDY_TYPE 15(6), 848-860) ST:STUDY_SUMMARY This is a time restricted feeding study (see M Hatori, et al. Cell Metabolism ST:STUDY_SUMMARY 15(6), 848-860). Briefly, there are four groups of mice: (1) NA mice are on a ST:STUDY_SUMMARY chow ad libitum diet; (2) FA mice are on a high fat ad libitum diet (i.e. Diet ST:STUDY_SUMMARY obesity); (3) FT mice are on a high fat time restricted diet (i.e. only eat for ST:STUDY_SUMMARY hours/day and fast for 16); and (4) CDKO mice which are cry knock out mice on a ST:STUDY_SUMMARY chow ad libitum diet. We have collected stool from these four groups while they ST:STUDY_SUMMARY in the light and in the dark. Our expectation is that their stool metabolites ST:STUDY_SUMMARY be different at different times of the day. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@umich.edu ST:PHONE - ST:NUM_GROUPS 8 ST:TOTAL_SUBJECTS 31 #SUBJECT SU:SUBJECT_TYPE - SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0003687 S00014230 Time Restriction:ad libitum | diurnal cycle:day | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003688 S00014231 Time Restriction:ad libitum | diurnal cycle:day | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003689 S00014232 Time Restriction:ad libitum | diurnal cycle:day | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003690 S00014233 Time Restriction:ad libitum | diurnal cycle:day | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003679 S00014222 Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003680 S00014223 Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003681 S00014224 Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003682 S00014225 Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003702 S00014245 Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:CDKO SUBJECT_SAMPLE_FACTORS SU0003703 S00014246 Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:CDKO SUBJECT_SAMPLE_FACTORS SU0003704 S00014247 Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:CDKO SUBJECT_SAMPLE_FACTORS SU0003705 S00014248 Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:CDKO SUBJECT_SAMPLE_FACTORS SU0003691 S00014234 Time Restriction:ad libitum | diurnal cycle:night | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003692 S00014235 Time Restriction:ad libitum | diurnal cycle:night | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003693 S00014236 Time Restriction:ad libitum | diurnal cycle:night | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003694 S00014237 Time Restriction:ad libitum | diurnal cycle:night | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003683 S00014226 Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003684 S00014227 Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003685 S00014228 Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003686 S00014229 Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003706 S00014249 Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:CDKO SUBJECT_SAMPLE_FACTORS SU0003707 S00014250 Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:CDKO SUBJECT_SAMPLE_FACTORS SU0003708 S00014251 Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:CDKO SUBJECT_SAMPLE_FACTORS SU0003709 S00014252 Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:CDKO SUBJECT_SAMPLE_FACTORS SU0003695 S00014238 Time Restriction:time restricted | diurnal cycle:day | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003696 S00014239 Time Restriction:time restricted | diurnal cycle:day | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003697 S00014240 Time Restriction:time restricted | diurnal cycle:day | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003698 S00014241 Time Restriction:time restricted | diurnal cycle:day | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003699 S00014242 Time Restriction:time restricted | diurnal cycle:night | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003700 S00014243 Time Restriction:time restricted | diurnal cycle:night | Diet:high fat | mouse strain:BKGND SUBJECT_SAMPLE_FACTORS SU0003701 S00014244 Time Restriction:time restricted | diurnal cycle:night | Diet:high fat | mouse strain:BKGND #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Feces #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Bile-acids-2015-03-11.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - CH:METHODS_ID AQM040 CH:METHODS_FILENAME 2014-03-27-Bile_acid-full-ESN-insert.m.zip #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ACQUISITION_PARAMETERS_FILE 2014-03-27-Bile_acid-full-ESN-insert.m.zip AN:PROCESSING_PARAMETERS_FILE EX00278-Bile-Acids-Quant-method.m.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490 QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg sample MS_METABOLITE_DATA_START Samples S00014230 S00014231 S00014232 S00014233 S00014222 S00014223 S00014224 S00014225 S00014245 S00014246 S00014247 S00014248 S00014234 S00014235 S00014236 S00014237 S00014226 S00014227 S00014228 S00014229 S00014249 S00014250 S00014251 S00014252 S00014238 S00014239 S00014240 S00014241 S00014242 S00014243 S00014244 Factors Time Restriction:ad libitum | diurnal cycle:day | Diet:high fat | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:day | Diet:high fat | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:day | Diet:high fat | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:day | Diet:high fat | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:CDKO Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:CDKO Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:CDKO Time Restriction:ad libitum | diurnal cycle:day | Diet:normal | mouse strain:CDKO Time Restriction:ad libitum | diurnal cycle:night | Diet:high fat | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:night | Diet:high fat | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:night | Diet:high fat | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:night | Diet:high fat | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:BKGND Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:CDKO Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:CDKO Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:CDKO Time Restriction:ad libitum | diurnal cycle:night | Diet:normal | mouse strain:CDKO Time Restriction:time restricted | diurnal cycle:day | Diet:high fat | mouse strain:BKGND Time Restriction:time restricted | diurnal cycle:day | Diet:high fat | mouse strain:BKGND Time Restriction:time restricted | diurnal cycle:day | Diet:high fat | mouse strain:BKGND Time Restriction:time restricted | diurnal cycle:day | Diet:high fat | mouse strain:BKGND Time Restriction:time restricted | diurnal cycle:night | Diet:high fat | mouse strain:BKGND Time Restriction:time restricted | diurnal cycle:night | Diet:high fat | mouse strain:BKGND Time Restriction:time restricted | diurnal cycle:night | Diet:high fat | mouse strain:BKGND a-Muricholic acid 0.4387 7.9793 24.2860 0.1763 1.0876 20.4157 17.6540 17.4253 0.7455 37.7020 22.7902 33.1021 17.6245 0.3579 0.7302 0.6290 3.8753 2.4591 0.8596 0.3337 0.3642 0.4677 43.1995 27.6032 0.5894 65.8995 0.1358 12.7198 77.8016 0.4386 49.4953 b-Muricholic acid 6.3856 10.8537 33.7245 3.8152 4.9241 25.0175 26.1478 22.2363 3.0299 48.4846 26.0940 46.0755 26.9396 6.6412 3.6247 5.7044 12.0586 7.3385 4.5494 3.0200 4.8347 2.4385 50.9674 33.0252 2.1295 76.0978 2.1253 21.1481 90.2622 5.4627 62.5634 Chenodeoxycholic acid 0.0000 0.0000 18.3898 0.0000 0.0000 8.9006 4.8395 7.9431 0.0000 9.8767 14.8974 12.0217 4.9785 1.1489 0.0000 0.0000 1.8727 0.7558 0.0000 0.3501 0.0000 0.0000 30.5705 12.6868 0.4300 19.3577 19.7298 2.7919 58.0121 0.3551 8.9525 Cholic acid 1.7096 0.3594 30.2417 0.9464 0.4606 16.5842 12.8921 17.6275 0.2400 42.3877 25.3151 33.0234 13.1470 0.5466 0.4404 1.4155 4.0762 1.5314 0.5033 1.3378 0.7459 0.3309 77.5777 35.7752 0.3838 117.5786 0.2845 10.4168 103.5397 3.0901 55.9088 Deoxycholic acid 17.0392 123.9090 384.6825 14.7342 16.1942 237.7734 183.9650 206.2516 8.3491 164.9154 303.0140 324.1241 179.6263 20.3866 6.5149 17.3664 21.9414 20.8886 9.2491 4.0958 6.8310 4.0800 360.0362 179.3024 7.8872 243.5205 6.3252 135.2622 1065.1063 5.7681 318.4984 Glychocholic acid 0.0027 0.0004 0.0245 0.0018 0.0017 0.0044 0.0190 0.0090 0.0013 0.0971 0.0026 0.0201 0.0089 0.0018 0.0030 0.0026 0.0026 0.0009 0.0034 0.0006 0.0018 0.0013 0.0222 0.0111 0.0030 0.1221 0.0006 0.0131 0.0190 0.0013 0.0344 Glycochenodeoxycholic acid 0.0021 0.0016 0.0029 0.0016 0.0020 0.0010 0.0015 0.0047 0.0012 0.0045 0.0035 0.0024 0.0030 0.0014 0.0015 0.0016 0.0022 0.0017 0.0015 0.0014 0.0016 0.0013 0.0018 0.0020 0.0014 0.0038 0.0012 0.0030 0.0031 0.0014 0.0031 Glycodeoxycholic acid 0.0011 0.0026 0.0223 0.0014 0.0048 0.0038 0.0148 0.0036 0.0020 0.0380 0.0105 0.0160 0.0245 0.0026 0.0056 0.0062 0.0042 0.0054 0.0025 0.0009 0.0006 0.0014 0.0073 0.0051 0.0026 0.0173 0.0020 0.0091 0.0238 0.0014 0.0292 GLYCO-HYODEOXYCHOLIC ACID/GLYCOURSODEOXYCHOLIC ACID 0.0001 0.0014 0.0096 0.0002 0.0011 0.0034 0.0050 0.0037 0.0002 0.0110 0.0024 0.0065 0.0052 0.0012 0.0009 0.0018 0.0006 0.0007 0.0005 0.0002 0.0000 0.0002 0.0052 0.0039 0.0007 0.0065 0.0002 0.0051 0.0041 0.0001 0.0096 Glycolithocholic acid 0.0000 0.3440 3.1688 0.0000 0.1256 0.8342 1.5992 0.1542 0.1331 2.7781 0.3166 0.7828 2.7988 0.7879 0.4000 0.2710 0.1515 0.8965 0.3682 0.0000 0.0656 0.1220 1.7521 0.7656 0.1488 0.5718 0.0869 0.7377 2.0209 0.0436 1.0931 g-Muricholic acid (hyocholic acid) 0.0000 0.8480 3.2028 0.0000 0.0579 2.3573 1.5390 1.9517 0.0467 2.1928 4.0208 3.1341 1.5101 0.1419 0.0000 0.0000 0.2731 0.1993 0.0745 0.0000 0.0000 0.0000 5.4475 3.5853 0.0281 3.8118 0.0000 0.9581 6.8988 0.0000 4.8329 Hyodeoxycholic acid 1.2527 54.9589 137.1496 0.8299 5.0887 104.8084 67.4455 89.6306 1.4582 49.1730 118.8248 105.9186 62.5374 3.1611 1.6623 1.5122 5.7597 6.4538 1.8774 0.7010 0.8407 0.8242 135.2304 79.8565 1.6499 79.4412 0.3087 45.9897 223.6952 0.7638 115.1579 Lithocholic acid 1.3286 15.9786 21.4998 1.3962 2.3463 20.2621 18.6104 19.5027 1.2113 17.7780 21.6029 19.9286 14.4639 3.0811 1.1955 1.9365 2.3708 3.1762 1.6893 0.6172 1.2139 1.4892 21.1913 17.1575 1.3105 14.4477 0.9767 10.7419 23.0197 0.6589 14.8582 Taurochenodeoxycholic acid 0.0131 0.0770 0.3204 0.0147 0.0316 0.3369 0.1895 0.3973 0.0070 0.3829 0.2374 0.1873 0.0397 0.0371 0.0272 0.0302 0.0524 0.0354 0.0397 0.0224 0.0083 0.0330 0.3148 0.2840 0.0296 1.7013 0.0134 0.2050 0.2069 0.0171 0.1449 Taurocholic acid 0.1134 0.3197 3.7386 0.1690 0.1161 0.8327 1.0869 2.2174 0.0268 5.2223 0.9883 0.6023 0.4616 0.1637 0.0937 0.1548 0.3124 0.1545 0.3036 0.0727 0.0679 0.1200 1.8651 1.9606 0.1500 9.4102 0.1108 1.3753 0.8136 0.0644 0.7004 Taurodeoxycholic acid 0.0149 0.0882 0.3737 0.0117 0.0399 0.3832 0.2194 0.4632 0.0071 0.4379 0.2749 0.2407 0.0465 0.0477 0.0308 0.0373 0.0622 0.0480 0.0475 0.0268 0.0101 0.0075 1.9968 1.5441 0.0362 1.9498 0.0151 0.2341 0.2424 0.0201 0.1657 Taurolithocolic acid 0.0000 0.0000 0.2186 0.0000 0.0106 0.0269 0.1309 0.0500 0.0008 0.1797 0.0617 0.0226 0.0172 0.0046 0.0038 0.0000 0.0180 0.0075 0.0072 0.0000 0.0026 0.0059 0.0632 0.0673 0.0058 0.1053 0.0000 0.0250 0.0195 0.0023 0.0221 Tauroursodeoxycholic acid/Tauro-hyodeoxycholic acid 0.0258 0.3992 2.3705 0.0236 0.0638 1.6673 1.4912 2.0850 0.0146 1.7238 1.1309 1.0145 0.2124 0.0692 0.0709 0.0677 0.1206 0.0855 0.0418 0.0444 0.0137 0.0561 1.5426 1.4825 0.0585 5.8588 0.0253 1.2248 0.8305 0.0226 1.0633 w-Muricholic acid 7.7165 21.3580 50.8936 4.8492 12.6581 40.2688 32.6967 35.3520 5.7967 53.6071 46.9861 57.7588 33.1547 14.2460 8.0432 7.9301 16.3772 12.3835 8.1251 4.7852 6.6345 4.9257 82.8906 52.3476 5.1235 91.7532 2.5496 28.3618 104.5645 6.2739 77.1003 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type a-Muricholic acid 53477700 aMCA UM_Target_ID b-Muricholic acid 5283853 bMCA UM_Target_ID Chenodeoxycholic acid 10133 CDCA UM_Target_ID Cholic acid 221493 CA UM_Target_ID Deoxycholic acid 6742 DCA UM_Target_ID Glychocholic acid 10140 GCA UM_Target_ID Glycochenodeoxycholic acid 9548796 GCDCA UM_Target_ID Glycodeoxycholic acid 18602029 GDCA UM_Target_ID GLYCO-HYODEOXYCHOLIC ACID/GLYCOURSODEOXYCHOLIC ACID 12310288 GHDCA/GUDCA UM_Target_ID Glycolithocholic acid 115245 GLCA UM_Target_ID g-Muricholic acid (hyocholic acid) 92805 HCA UM_Target_ID Hyodeoxycholic acid 16219478 HDCA UM_Target_ID Lithocholic acid 9903 LCA UM_Target_ID Taurochenodeoxycholic acid 54608447 TCDCA UM_Target_ID Taurocholic acid 6675 TCA UM_Target_ID Taurodeoxycholic acid 10594 TDCA UM_Target_ID Taurolithocolic acid 10595 TLCA UM_Target_ID Tauroursodeoxycholic acid/Tauro-hyodeoxycholic acid 9848818 THDCA/TUDCA UM_Target_ID w-Muricholic acid 5283851 wMCA UM_Target_ID METABOLITES_END #END