#METABOLOMICS WORKBENCH araskind_20150609_9971371_mwtab.txt DATATRACK_ID:294 STUDY_ID:ST000193 ANALYSIS_ID:AN000295 PROJECT_ID:PR000164 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE BAF60a LKO Liver specific knockout Bile acid PR:PROJECT_SUMMARY Regulation of Bile acid metabolism by hepatic BAF60a PR:INSTITUTE University of Michigan PR:DEPARTMENT Life Sciences Institute PR:LABORATORY Lin Lab PR:LAST_NAME Lin PR:FIRST_NAME Jiandie PR:ADDRESS Ann Arbor, MI PR:EMAIL jdlin@umich.edu PR:PHONE 734-615-3512 #STUDY ST:STUDY_TITLE BAF60a LKO Liver specific knockout Bile acid ST:STUDY_TYPE Twelve weeks old WT and BAF60a Liver specfific knockout mice were fed with high ST:STUDY_TYPE diet for 8 weeks. Mice were sacrified after 4 h of starvation. Blood samples ST:STUDY_TYPE harvested by heart puncture for plasma. Liver were dissected and immediately ST:STUDY_TYPE in liquid nitrogen. All the samples were kept in -80oC freezer for long term ST:STUDY_SUMMARY Twelve weeks old WT and BAF60a Liver specfific knockout mice were fed with high ST:STUDY_SUMMARY diet for 8 weeks. Mice were sacrified after 4 h of starvation. Blood samples ST:STUDY_SUMMARY harvested by heart puncture for plasma. Liver were dissected and immediately ST:STUDY_SUMMARY in liquid nitrogen. All the samples were kept in -80oC freezer for long term ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@umich.edu ST:PHONE - ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 18 #SUBJECT SU:SUBJECT_TYPE - SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0003713 S00014253 Genotype:Knockout SUBJECT_SAMPLE_FACTORS SU0003718 S00014258 Genotype:Knockout SUBJECT_SAMPLE_FACTORS SU0003719 S00014259 Genotype:Knockout SUBJECT_SAMPLE_FACTORS SU0003721 S00014261 Genotype:Knockout SUBJECT_SAMPLE_FACTORS SU0003713 S00014262 Genotype:Knockout SUBJECT_SAMPLE_FACTORS SU0003718 S00014267 Genotype:Knockout SUBJECT_SAMPLE_FACTORS SU0003719 S00014268 Genotype:Knockout SUBJECT_SAMPLE_FACTORS SU0003721 S00014270 Genotype:Knockout SUBJECT_SAMPLE_FACTORS SU0003714 S00014254 Genotype:Wild type SUBJECT_SAMPLE_FACTORS SU0003715 S00014255 Genotype:Wild type SUBJECT_SAMPLE_FACTORS SU0003716 S00014256 Genotype:Wild type SUBJECT_SAMPLE_FACTORS SU0003717 S00014257 Genotype:Wild type SUBJECT_SAMPLE_FACTORS SU0003720 S00014260 Genotype:Wild type SUBJECT_SAMPLE_FACTORS SU0003714 S00014263 Genotype:Wild type SUBJECT_SAMPLE_FACTORS SU0003715 S00014264 Genotype:Wild type SUBJECT_SAMPLE_FACTORS SU0003716 S00014265 Genotype:Wild type SUBJECT_SAMPLE_FACTORS SU0003717 S00014266 Genotype:Wild type SUBJECT_SAMPLE_FACTORS SU0003720 S00014269 Genotype:Wild type #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Liver #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Bile-acids-2015-03-11.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME - CH:COLUMN_NAME Waters Acquity BEH C18 (50 x 2.1mm, 1.7um) CH:METHODS_ID AQM040 CH:METHODS_FILENAME 2014-03-27-Bile_acid-full-ESN-insert.m.zip #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ACQUISITION_PARAMETERS_FILE 2014-03-27-Bile_acid-full-ESN-insert.m.zip AN:PROCESSING_PARAMETERS_FILE EX00282-Bile-Acids-Quant-method.m.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6490 QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol MS_METABOLITE_DATA_START Samples S00014253 S00014258 S00014259 S00014261 S00014262 S00014267 S00014268 S00014270 S00014254 S00014255 S00014256 S00014257 S00014260 S00014263 S00014264 S00014265 S00014266 S00014269 Factors Genotype:Knockout Genotype:Knockout Genotype:Knockout Genotype:Knockout Genotype:Knockout Genotype:Knockout Genotype:Knockout Genotype:Knockout Genotype:Wild type Genotype:Wild type Genotype:Wild type Genotype:Wild type Genotype:Wild type Genotype:Wild type Genotype:Wild type Genotype:Wild type Genotype:Wild type Genotype:Wild type a-Muricholic acid 4.1789 4.3054 3.1754 0.5348 23.0869 0.5703 4.3232 7.0726 12.1987 1.5295 1.6015 1.1870 0.3936 0.3231 0.8312 0.3083 0.1068 b-Muricholic acid 23.6882 438.3041 205.3238 37.6853 2.2432 228.2595 17.0742 21.4949 53.1749 96.7909 12.0752 16.1168 13.5265 4.2627 7.9110 9.6248 3.3978 3.2480 Chenodeoxycholic acid 8.1323 7.7241 25.1702 0.2705 0.2562 0.2466 0.4360 9.9376 23.6225 0.8570 11.4653 0.2378 0.2431 0.3710 0.2483 0.2968 Cholic acid 7.5341 188.5010 82.3430 10.2012 0.2961 467.4344 7.9031 34.3804 21.6475 79.4950 4.4171 21.9559 10.3629 0.2905 0.7482 0.4359 1.2383 0.5044 Deoxycholic acid 1.0221 53.9350 51.3462 57.6998 0.0078 0.0916 0.0306 0.1446 1.1508 12.7584 0.8756 7.1890 13.0157 0.0066 0.0194 0.0058 0.0105 0.0151 Glychocholic acid 1.6976 2.3255 1.3950 0.0984 0.2866 3.3656 0.5211 1.9494 0.6497 0.5871 0.0631 0.1999 0.1736 0.4694 0.3171 1.1522 0.5884 0.4692 Glycochenodeoxycholic acid 1.5577 1.0765 1.6516 1.7169 0.1233 0.2148 0.1348 0.2692 1.9557 1.4272 2.0088 1.7692 1.5663 0.1007 0.0827 0.1517 0.1098 0.0976 Glycodeoxycholic acid 0.1739 0.0834 0.1533 0.0695 0.0958 0.0195 0.1927 0.1164 0.0024 0.0158 0.0034 0.0102 0.1059 GLYCO-HYODEOXYCHOLIC ACID/GLYCOURSODEOXYCHOLIC ACID 0.1482 0.0699 0.1116 0.0260 0.0496 0.1263 0.0406 0.1648 0.0861 0.0347 0.0305 0.0151 0.0520 0.0266 0.0909 0.0169 0.0173 Glycolithocholic acid 28.0672 4.8878 g-Muricholic acid (hyocholic acid) 0.1487 0.1157 0.2587 0.2175 4.4054 0.1051 Hyodeoxycholic acid 6.9453 72.8349 98.4861 26.7142 0.1438 1.0115 0.6639 0.9453 15.5307 50.0665 2.3639 14.9229 4.7512 0.3199 0.3108 0.3636 0.2650 0.1529 Lithocholic acid 3.8647 5.3238 4.4709 5.5767 0.1186 4.5885 4.7778 4.3239 5.1130 3.4154 0.0446 Taurochenodeoxycholic acid 12.2485 3.1990 9.7440 3.9331 10.8953 12.1795 10.2191 62.5498 3.1704 3.0545 5.1274 2.2880 3.5997 9.2642 8.0246 9.6491 9.0051 4.6705 Taurocholic acid 40.0657 18.0807 44.4774 10.5707 81.0582 408.4611 123.7122 410.3999 12.9477 250.2863 30.7240 12.2931 17.1861 77.3425 122.8872 125.6973 121.6505 89.7196 Taurodeoxycholic acid 13.5764 0.7674 0.7996 0.6414 1.7718 0.9648 11.2209 68.9097 3.4557 4.0120 6.7713 2.4884 3.9312 10.0582 8.7603 10.5946 5.2914 4.8132 Taurolithocolic acid 0.1941 0.3168 0.2043 0.6264 0.6744 2.3481 0.2658 0.2246 0.2962 0.2800 0.2677 0.0237 0.3139 Tauroursodeoxycholic acid/Tauro-hyodeoxycholic acid 29.8597 7.8198 55.9587 16.4705 20.6261 38.1924 43.2647 131.0828 18.2386 44.3355 35.6690 20.6891 19.3819 22.8550 26.7984 30.3775 30.4527 13.8858 w-Muricholic acid 103.1403 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type a-Muricholic acid 53477700 aMCA UM_Target_ID b-Muricholic acid 5283853 bMCA UM_Target_ID Chenodeoxycholic acid 10133 CDCA UM_Target_ID Cholic acid 221493 CA UM_Target_ID Deoxycholic acid 6742 DCA UM_Target_ID Glychocholic acid 10140 GCA UM_Target_ID Glycochenodeoxycholic acid 9548796 GCDCA UM_Target_ID Glycodeoxycholic acid 18602029 GDCA UM_Target_ID GLYCO-HYODEOXYCHOLIC ACID/GLYCOURSODEOXYCHOLIC ACID 12310288 GHDCA/GUDCA UM_Target_ID Glycolithocholic acid 115245 GLCA UM_Target_ID g-Muricholic acid (hyocholic acid) 92805 HCA UM_Target_ID Hyodeoxycholic acid 16219478 HDCA UM_Target_ID Lithocholic acid 9903 LCA UM_Target_ID Taurochenodeoxycholic acid 54608447 TCDCA UM_Target_ID Taurocholic acid 6675 TCA UM_Target_ID Taurodeoxycholic acid 10594 TDCA UM_Target_ID Taurolithocolic acid 10595 TLCA UM_Target_ID Tauroursodeoxycholic acid/Tauro-hyodeoxycholic acid 9848818 THDCA/TUDCA UM_Target_ID w-Muricholic acid 5283851 wMCA UM_Target_ID METABOLITES_END #END