#METABOLOMICS WORKBENCH rgn5011_20150608_9529801_mwtab.txt DATATRACK_ID:289 TEXT OUTPUT STUDY_ID:ST000225 ANALYSIS_ID:AN000335 PROJECT_ID:PR000120 VERSION 1 CREATED_ON 10-02-2015 #PROJECT PR:PROJECT_TITLE Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts PR:PROJECT_TITLE Gut Microbiota-Host Metabolic Homeostasis in Mice PR:INSTITUTE Pennsylvania State University PR:DEPARTMENT Molecular Toxicology PR:LAST_NAME Andrew PR:FIRST_NAME Patterson PR:EMAIL adp117@PSU.EDU, RGN5011@psu.edu PR:PROJECT_SUMMARY - PR:PHONE - PR:ADDRESS - #STUDY ST:STUDY_TITLE Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts ST:STUDY_TITLE Gut Microbiota-Host Metabolic Homeostasis in Mice ST:STUDY_TYPE drug dosage ST:STUDY_SUMMARY Mice were dosed with TCDF for 5 days ST:INSTITUTE Pennsylvania State University ST:DEPARTMENT Molecular Toxicology ST:LAST_NAME Patterson ST:FIRST_NAME Andrew ST:EMAIL adp117@PSU.EDU ST:PHONE - ST:ADDRESS - #SUBJECT SU:SUBJECT_TYPE mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57BL/6J; Wild Type and Ahr-/- SU:AGE_OR_AGE_RANGE 6 weeks old SU:GENDER Males #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - Control cecal content Gender:Male | Genotype:Control SUBJECT_SAMPLE_FACTORS - Control fecal Gender:Male | Genotype:Control SUBJECT_SAMPLE_FACTORS - Control Liver Gender:Male | Genotype:Control SUBJECT_SAMPLE_FACTORS - Treated Cecal Content Gender:Male | Genotype:Tcdf Treated SUBJECT_SAMPLE_FACTORS - Treated Cecum Gender:Male | Genotype:Tcdf Treated SUBJECT_SAMPLE_FACTORS - Treated Duodenum Gender:Male | Genotype:Tcdf Treated SUBJECT_SAMPLE_FACTORS - Treated Fecal Gender:Male | Genotype:Tcdf Treated SUBJECT_SAMPLE_FACTORS - Treated Ileum Gender:Male | Genotype:Tcdf Treated SUBJECT_SAMPLE_FACTORS - Treated Jejunum Gender:Male | Genotype:Tcdf Treated SUBJECT_SAMPLE_FACTORS - Treated Liver Gender:Male | Genotype:Tcdf Treated #COLLECTION CO:SAMPLE_TYPE Feces, liver, cecal contents, and intestine CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY Wild type and AHR knock out mice (5-6 per group) were treated with TCDF for 5 TR:TREATMENT_SUMMARY days TR:TREATMENT Dietary TR:TREATMENT_COMPOUND TCDF TR:TREATMENT_ROUTE Oral TR:TREATMENT_DOSE 24 µg/kg body weight #SAMPLEPREP SP:SAMPLEPREP_PROTOCOL_FILENAME NMR_metabolomics_protocol.docx SP:PROCESSING_METHOD Homogenization SP:PROCESSING_STORAGE_CONDITIONS -20 SP:EXTRACTION_METHOD Methanol/Water SP:SAMPLE_RESUSPENSION D2O SP:SAMPLE_SPIKING TSP-d4 SP:ORGAN Liver, small and large intestine SP:SAMPLEPREP_SUMMARY - #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:ANALYSIS_TYPE NMR #NMR NM:INSTRUMENT_TYPE Bruker Avance III 600 MHz spectrometer NM:NMR_EXPERIMENT_TYPE NOESY PR1D NM:SPECTROMETER_FREQUENCY 600 MHz NM:NMR_SOLVENT D2O NM:INSTRUMENT_NAME Bruker Avance III #END