#METABOLOMICS WORKBENCH rgn5011_20150618_9618511_mwtab.txt DATATRACK_ID:327 TEXT OUTPUT STUDY_ID:ST000226 ANALYSIS_ID:AN000336 PROJECT_ID:PR000120 VERSION 1 CREATED_ON 10-02-2015 #PROJECT PR:PROJECT_TITLE Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts PR:PROJECT_TITLE Gut Microbiota-Host Metabolic Homeostasis in Mice PR:INSTITUTE Pennsylvania State University PR:DEPARTMENT Molecular Toxicology PR:LAST_NAME Andrew PR:FIRST_NAME Patterson PR:EMAIL adp117@PSU.EDU, RGN5011@psu.edu PR:PROJECT_SUMMARY - PR:PHONE - PR:ADDRESS - #STUDY ST:STUDY_TITLE Metabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental ST:STUDY_TITLE Chemicals Induces Systemic Metabolic Dysfunction in Mice (Part I) ST:STUDY_TYPE drug dosage ST:STUDY_SUMMARY 12 mice were acclimatized for a week, taught to eat dough pills then half were ST:STUDY_SUMMARY treated with TCDF per pill per day for 5 days ST:STUDY_SUMMARY Also 10 AHR -/- mice were used and treated the same way ST:INSTITUTE Pennsylvania State University ST:DEPARTMENT Molecular Toxicology ST:LAST_NAME Patterson ST:FIRST_NAME Andrew ST:EMAIL adp117@PSU.EDU ST:PHONE - ST:ADDRESS - #SUBJECT SU:SUBJECT_TYPE mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57BL/6J; Wild Type and Ahr-/- SU:AGE_OR_AGE_RANGE 6 weeks old SU:GENDER Males #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - Treated serum_Ahr- Gender:Male | Genotype:Ahr -/- | Treatment:TCDF SUBJECT_SAMPLE_FACTORS - Treated Liver Gender:Male | Genotype:Ahr +/+ | Treatment:TCDF SUBJECT_SAMPLE_FACTORS - Treated serum_Ahr+ Gender:Male | Genotype:Ahr +/+ | Treatment:TCDF SUBJECT_SAMPLE_FACTORS - Control serum_Ahr- Gender:Male | Genotype:Ahr -/- | Treatment:Vehicle SUBJECT_SAMPLE_FACTORS - Control Liver Gender:Male | Genotype:Ahr +/+ | Treatment:Vehicle SUBJECT_SAMPLE_FACTORS - Control serum_Ahr+ Gender:Male | Genotype:Ahr +/+ | Treatment:Vehicle #COLLECTION CO:SAMPLE_TYPE Feces, liver, cecal contents, intestine and blood (only liver and blood tested CO:SAMPLE_TYPE with NRM) CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY Wild type and AHR knock out mice (12 wild types and 10 AHR knock out) were TR:TREATMENT_SUMMARY treated with TCDF for 5 days TR:TREATMENT Dietary TR:TREATMENT_COMPOUND TCDF TR:TREATMENT_ROUTE Oral TR:TREATMENT_DOSE 24 µg/kg body weight for AHR and 5 µg/kg body weight for wild type #SAMPLEPREP SP:SAMPLEPREP_PROTOCOL_FILENAME NMR_metabolomics_protocol.docx SP:PROCESSING_METHOD Homogenization SP:PROCESSING_STORAGE_CONDITIONS -20 SP:EXTRACTION_METHOD Methanol/Water SP:SAMPLE_RESUSPENSION D2O SP:SAMPLE_SPIKING TSP-d4 SP:ORGAN Liver, small and large intestine SP:SAMPLEPREP_SUMMARY - #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:ANALYSIS_TYPE NMR #NMR NM:INSTRUMENT_TYPE Bruker Avance III 600 MHz spectrometer NM:NMR_EXPERIMENT_TYPE NOESY PR1D NM:SPECTROMETER_FREQUENCY 600 MHz NM:NMR_SOLVENT D2O NM:INSTRUMENT_NAME Bruker Avance III #END