#METABOLOMICS WORKBENCH Data-spleen DATATRACK_ID:406 STUDY_ID:ST000261 ANALYSIS_ID:AN000416 VERSION 1 CREATED_ON 08-09-2023 #PROJECT PR:PROJECT_TITLE 1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation PR:PROJECT_SUMMARY 1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation PR:INSTITUTE Pacific Northwest National Laboratory PR:DEPARTMENT Fundamental & Computational Sciences PR:LAST_NAME Hu PR:FIRST_NAME Jianzhi PR:ADDRESS -- PR:EMAIL jianzhi.hu@pnnl.gov PR:PHONE -- PR:DOI http://dx.doi.org/10.21228/M80C7B #STUDY ST:STUDY_TITLE 1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation ST:STUDY_TYPE Tissue Extracts Comparison ST:STUDY_SUMMARY Tissue extracts from exposure to gamma radiation mouse spleen and controls were ST:STUDY_SUMMARY compared via NMR based metabolomic analysis ST:INSTITUTE Pacific Northwest National Laboratory ST:DEPARTMENT Fundamental & Computational Sciences ST:LAST_NAME Hu ST:FIRST_NAME Jianzhi ST:ADDRESS -- ST:EMAIL jianzhi.hu@pnnl.gov ST:PHONE -- ST:SUBMIT_DATE 2015-10-26 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57BL/6J SU:SPECIES_GROUP Mammal #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - P041 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 SUBJECT_SAMPLE_FACTORS - P042 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 SUBJECT_SAMPLE_FACTORS - P043 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 SUBJECT_SAMPLE_FACTORS - P044 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 SUBJECT_SAMPLE_FACTORS - P045 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 SUBJECT_SAMPLE_FACTORS - P046 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 SUBJECT_SAMPLE_FACTORS - P047 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 SUBJECT_SAMPLE_FACTORS - P048 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 SUBJECT_SAMPLE_FACTORS - P341 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 SUBJECT_SAMPLE_FACTORS - P342 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 SUBJECT_SAMPLE_FACTORS - P343 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 SUBJECT_SAMPLE_FACTORS - P344 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 SUBJECT_SAMPLE_FACTORS - P345 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 SUBJECT_SAMPLE_FACTORS - P741 Disease? (0, no; 1, yes):1 | Dosage (Gy):7.8 SUBJECT_SAMPLE_FACTORS - P742 Disease? (0, no; 1, yes):1 | Dosage (Gy):7.8 SUBJECT_SAMPLE_FACTORS - P743 Disease? (0, no; 1, yes):1 | Dosage (Gy):7.8 SUBJECT_SAMPLE_FACTORS - P744 Disease? (0, no; 1, yes):1 | Dosage (Gy):7.8 #COLLECTION CO:COLLECTION_SUMMARY Spleens were dissected and snap-frozen at -80°C before NMR spectroscopy and CO:COLLECTION_SUMMARY morphology analysis. CO:SAMPLE_TYPE Tissue #TREATMENT TR:TREATMENT_SUMMARY A total of 17 seven-week-old C57BL/6 female mice were randomly grouped before TR:TREATMENT_SUMMARY total body being exposed to gamma irradiation using a high activity gamma source TR:TREATMENT_SUMMARY (1250 keV 60Co). The linear energy transfer (LET) associated with these fields TR:TREATMENT_SUMMARY is in the range of 0.2-2 keV/?m. For the total body gamma radiation, the animals TR:TREATMENT_SUMMARY were isolated to the corner of their polymer cages, placed at a minimum of 100 TR:TREATMENT_SUMMARY cm from the collimated 6000 Ci 60Co source, and then irradiated to the proposed TR:TREATMENT_SUMMARY dosage. The resulting absorbed dose rate at approximately 600 mg/cm2 depth was TR:TREATMENT_SUMMARY 0.83Gy/min relative to tissue. Groups of mice were exposed to radiation doses of TR:TREATMENT_SUMMARY 0Gy (control, n=8), 3.0Gy (n=5) and 7.8Gy (n=4). After 4 days post exposure, TR:TREATMENT_SUMMARY mice were sacrificed with 70/30 CO2/O2 . #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Cold Methanol/H2O/Chloroform Extraction SP:SAMPLEPREP_PROTOCOL_COMMENTS Extraction protocol: Step 1: Weight each intact frozen tissue and add 0.25 ml SP:SAMPLEPREP_PROTOCOL_COMMENTS methanol and 0.053 ml deionized water for each sample, after homogenizing in ice SP:SAMPLEPREP_PROTOCOL_COMMENTS bath using the Tissue Tearor (Model 985-370, BioSpec Products, Inc.), then SP:SAMPLEPREP_PROTOCOL_COMMENTS following by vortexing the mixture and then adding 0.125 ml chloroform, SP:SAMPLEPREP_PROTOCOL_COMMENTS vortexing again. Step 2: 0.125 ml chloroform and 0.125 ml deionized water were SP:SAMPLEPREP_PROTOCOL_COMMENTS added to the sample and then vortexed again, following by transferring different SP:SAMPLEPREP_PROTOCOL_COMMENTS layers into glass vials separately with syringes after the mixture being left on SP:SAMPLEPREP_PROTOCOL_COMMENTS ice for 15 min and centrifuged at 6,000 rpm for 16 min at 4 °C. Finally, the SP:SAMPLEPREP_PROTOCOL_COMMENTS MeOH/H2O layer solvents (hydrophilic metabolites) were removed by employing the SP:SAMPLEPREP_PROTOCOL_COMMENTS lyophilizer. And the extracts were stored at ?80 °C freezer before performing SP:SAMPLEPREP_PROTOCOL_COMMENTS NMR measurements. #CHROMATOGRAPHY #ANALYSIS AN:ANALYSIS_TYPE NMR AN:SOFTWARE_VERSION Vnmrj/ 4.0; Chenomx/8.1 #NMR NM:INSTRUMENT_NAME Varian 600 MHz NM:INSTRUMENT_TYPE FT-NMR NM:NMR_EXPERIMENT_TYPE 1D NM:SPECTROMETER_FREQUENCY 600 MHZ NM:POWER_LEVEL Deuterium NM:RECEIVER_GAIN 0.05 mM NM:PRESATURATION_POWER_LEVEL 5mm HCN probe with Z axis-gradient NM:CHEMICAL_SHIFT_REF_CPD D2O NM:TEMPERATURE 5mm * 8 in NM:NUMBER_OF_SCANS Gradient shim NM:DUMMY_SCANS PRESAT NM:ACQUISITION_TIME presat NM:RELAXATION_DELAY 7.5 us NM:ZERO_FILLING DSS NM:APODIZATION 293 K NM:BASELINE_CORRECTION_METHOD 19968 #NMR_METABOLITE_DATA NMR_METABOLITE_DATA:UNITS uM NMR_METABOLITE_DATA_START Samples P041 P042 P043 P044 P045 P046 P047 P048 P341 P342 P343 P344 P345 P741 P742 P743 P744 Factors Disease? (0, no; 1, yes):0 | Dosage (Gy):0 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 Disease? (0, no; 1, yes):0 | Dosage (Gy):0 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 Disease? (0, no; 1, yes):1 | Dosage (Gy):3 Disease? (0, no; 1, yes):1 | Dosage (Gy):7.8 Disease? (0, no; 1, yes):1 | Dosage (Gy):7.8 Disease? (0, no; 1, yes):1 | Dosage (Gy):7.8 Disease? (0, no; 1, yes):1 | Dosage (Gy):7.8 2-Aminobutyrate 9.0000 15.3000 9.6000 8.7000 15.7000 20.6000 19.7000 12.5000 26.1000 31.5000 27.7000 27.0000 23.5000 25.2000 22.2000 25.0000 19.9000 2-Oxoglutarate 8.8000 16.8000 9.5000 10.2000 16.0000 20.0000 21.3000 12.1000 28.1000 28.3000 25.1000 21.9000 21.9000 26.8000 20.3000 33.8000 31.6000 3-Hydroxybutyrate 17.4000 27.3000 18.8000 14.6000 29.2000 37.1000 28.3000 20.0000 31.9000 32.2000 41.9000 25.8000 31.0000 12.2000 12.1000 15.7000 15.6000 3-Hydroxyisobutyrate 1.9000 3.6000 2.0000 1.5000 2.6000 3.6000 2.8000 2.0000 2.3000 2.3000 2.4000 2.5000 2.0000 3.3000 2.4000 3.7000 2.8000 Adenosine 4.8000 5.3000 3.5000 6.4000 7.3000 8.9000 10.2000 8.5000 7.5000 10.9000 7.2000 9.1000 6.3000 4.4000 3.7000 4.6000 3.7000 ADP 3.8000 6.2000 4.8000 3.5000 7.6000 8.8000 10.5000 6.7000 4.7000 4.6000 4.9000 3.8000 4.3000 3.2000 3.9000 5.7000 3.6000 Alanine 18.1000 38.8000 18.9000 19.9000 40.2000 57.7000 46.4000 33.4000 54.1000 56.6000 49.3000 66.7000 55.3000 73.9000 63.3000 77.9000 60.9000 AMP 10.1000 15.2000 7.2000 4.3000 18.7000 21.3000 11.8000 10.2000 15.1000 14.4000 12.9000 15.8000 12.0000 9.5000 7.5000 13.0000 12.6000 Arginine 11.3000 19.6000 12.5000 11.6000 22.0000 32.0000 25.7000 20.2000 39.6000 36.5000 32.2000 39.3000 36.4000 31.7000 31.7000 46.8000 32.0000 Ascorbate 55.6000 97.3000 67.3000 44.8000 99.8000 102.8000 112.0000 86.9000 98.3000 103.3000 99.4000 81.7000 102.9000 31.3000 58.1000 66.4000 96.5000 Aspartate 61.1000 93.0000 62.1000 47.2000 104.8000 167.0000 150.4000 100.6000 115.7000 141.7000 124.8000 135.9000 135.3000 91.4000 112.5000 123.6000 111.5000 ATP 4.2000 3.5000 3.5000 1.9000 2.3000 2.1000 7.5000 2.8000 3.5000 2.5000 3.8000 2.3000 2.8000 4.7000 5.5000 5.8000 5.8000 Betaine 11.6000 24.7000 9.3000 8.3000 18.8000 18.1000 21.2000 15.6000 13.8000 20.2000 13.2000 8.0000 8.9000 9.0000 10.1000 16.0000 7.9000 Choline 12.5000 28.2000 14.5000 9.9000 17.6000 29.0000 32.1000 22.1000 14.9000 15.7000 16.2000 15.4000 19.5000 23.8000 21.8000 26.5000 23.1000 Citrate 0.7000 3.7000 0.8000 1.1000 1.5000 2.7000 3.7000 1.3000 4.0000 3.2000 3.1000 3.3000 3.1000 2.3000 2.9000 3.5000 2.3000 Creatine 4.7000 8.2000 5.2000 3.4000 7.9000 9.1000 10.0000 8.6000 19.4000 22.5000 18.6000 19.7000 21.1000 14.4000 15.3000 21.8000 20.4000 Creatine phosphate 3.7000 6.8000 3.4000 2.7000 4.9000 9.7000 6.3000 5.6000 7.3000 8.3000 6.5000 7.6000 7.2000 7.5000 6.8000 9.9000 9.1000 Creatinine 2.9000 6.5000 5.3000 3.1000 6.1000 9.9000 8.7000 5.4000 7.3000 7.1000 9.1000 7.9000 7.7000 11.2000 10.9000 14.0000 13.2000 Cytidine 2.3000 4.4000 2.5000 1.7000 2.6000 3.9000 3.6000 3.3000 3.0000 3.0000 3.1000 3.0000 3.6000 2.3000 2.2000 3.8000 2.1000 Ethanolamine 17.4000 28.3000 14.1000 16.8000 20.9000 42.5000 33.0000 18.8000 30.9000 31.2000 29.8000 18.4000 30.3000 18.2000 29.4000 35.1000 22.7000 Fucose 8.0000 9.7000 7.6000 6.0000 12.7000 18.0000 15.3000 12.9000 20.5000 15.9000 23.2000 15.3000 14.5000 11.1000 12.0000 27.4000 18.3000 Fumarate 0.4000 1.0000 0.7000 0.4000 0.8000 1.1000 0.9000 0.8000 1.2000 1.3000 1.0000 0.8000 0.8000 0.7000 0.9000 0.9000 0.5000 Glucose 9.9000 13.0000 8.3000 5.6000 18.0000 24.5000 22.3000 13.8000 15.1000 15.1000 18.9000 18.0000 23.4000 14.9000 25.8000 37.4000 36.6000 Glutamate 122.9000 206.8000 149.1000 96.8000 202.7000 346.4000 267.7000 229.0000 239.9000 267.7000 280.6000 271.2000 257.1000 166.2000 170.6000 200.0000 187.7000 ?-Glutamylphenylalanine 1.3000 2.2000 1.5000 1.4000 2.7000 3.4000 2.8000 2.1000 3.2000 3.5000 3.2000 2.5000 2.8000 3.1000 2.6000 4.0000 3.4000 Glutathione 11.2000 20.6000 13.7000 12.1000 16.1000 25.4000 25.7000 17.8000 35.7000 37.8000 35.0000 25.6000 27.8000 23.9000 34.8000 41.3000 29.7000 Glycerol 13.2000 18.6000 13.2000 10.9000 17.0000 21.9000 22.8000 15.1000 31.2000 36.9000 34.2000 27.5000 27.9000 28.9000 26.3000 46.8000 39.4000 Glycine 20.0000 42.5000 15.5000 16.9000 44.2000 55.0000 61.1000 39.3000 79.4000 76.0000 67.2000 80.7000 60.2000 26.5000 38.9000 66.7000 40.5000 GTP 4.4000 7.5000 4.9000 3.2000 7.3000 7.9000 6.9000 5.0000 6.1000 6.4000 6.4000 8.9000 8.8000 4.1000 3.0000 4.6000 4.6000 Histidine 1.7000 2.6000 1.4000 1.5000 1.9000 2.1000 2.8000 1.4000 2.0000 2.1000 2.0000 2.7000 1.5000 2.3000 2.5000 2.9000 2.9000 Hypoxanthine 3.4000 5.6000 2.9000 2.9000 4.7000 8.0000 5.5000 4.7000 5.2000 6.4000 5.5000 9.0000 9.6000 6.1000 6.0000 7.3000 7.3000 Inosine 0.7000 1.0000 5.3000 4.7000 9.7000 12.3000 11.5000 10.2000 2.5000 4.4000 4.9000 2.1000 2.7000 0.9000 4.0000 6.4000 1.5000 Isobutyrate 1.4000 2.1000 1.6000 1.6000 2.3000 3.6000 2.2000 1.5000 1.9000 2.0000 2.5000 2.1000 2.0000 1.8000 2.2000 2.8000 2.1000 Isocitrate 17.9000 34.4000 15.2000 22.8000 33.6000 36.9000 31.0000 30.3000 31.5000 40.7000 35.7000 33.2000 31.5000 38.3000 34.8000 43.3000 40.0000 Lactate 56.0000 119.7000 79.8000 61.2000 107.3000 156.3000 127.9000 107.9000 234.4000 249.3000 205.3000 229.6000 215.4000 204.0000 233.6000 289.5000 241.6000 Leucine 13.1000 21.3000 13.4000 12.4000 18.1000 21.6000 17.8000 13.9000 32.6000 34.9000 35.7000 36.1000 29.6000 49.8000 39.4000 49.7000 34.2000 Lysine 8.2000 10.3000 8.5000 8.8000 9.6000 12.1000 13.0000 10.1000 16.9000 18.8000 20.5000 18.7000 18.6000 12.1000 15.0000 18.4000 18.4000 Malate 12.2000 22.0000 14.6000 12.5000 23.2000 29.5000 32.2000 18.4000 36.4000 30.9000 33.9000 34.4000 33.9000 26.3000 23.7000 25.1000 28.7000 myo-Inositol 46.6000 70.8000 50.6000 37.3000 72.5000 98.2000 79.6000 65.3000 150.8000 134.9000 146.0000 155.0000 158.7000 148.2000 170.5000 194.8000 234.7000 Niacinamide 2.3000 4.4000 2.5000 1.3000 3.2000 3.6000 3.2000 1.9000 4.0000 4.5000 3.5000 4.1000 4.3000 3.5000 3.6000 4.5000 4.9000 O-Phosphoethanolamine 115.1000 188.1000 127.3000 102.6000 188.1000 251.5000 217.9000 164.7000 179.8000 204.3000 191.1000 204.2000 187.8000 128.7000 120.9000 144.3000 143.2000 Oxypurinol 103.7000 202.4000 133.4000 102.4000 154.4000 230.5000 316.4000 201.8000 113.2000 121.9000 132.6000 148.4000 108.1000 174.8000 186.9000 253.7000 194.9000 Phenylalanine 3.0000 4.7000 3.0000 1.7000 3.6000 6.6000 4.6000 3.7000 8.8000 9.0000 7.9000 10.2000 9.0000 15.9000 11.4000 13.9000 11.7000 p-Methylhistidine 1.0000 1.5000 1.0000 1.2000 1.8000 3.0000 2.3000 1.5000 3.3000 4.8000 2.5000 2.9000 4.1000 4.4000 4.1000 4.9000 3.0000 Serine 33.7000 64.5000 38.9000 31.5000 82.1000 104.8000 91.7000 73.5000 88.5000 95.1000 91.4000 72.3000 91.7000 80.1000 79.1000 102.9000 91.7000 sn-Glycero-3-phosphocholine 29.9000 63.1000 37.5000 30.2000 48.6000 89.7000 65.8000 51.3000 87.5000 80.2000 88.6000 101.7000 91.0000 58.4000 72.5000 78.5000 94.4000 ß-Alanine 11.1000 14.9000 11.8000 11.6000 15.9000 20.1000 25.4000 17.1000 19.1000 35.2000 29.8000 32.9000 19.1000 19.1000 21.8000 30.3000 24.7000 Succinate 9.0000 13.8000 9.3000 4.7000 9.8000 10.9000 13.4000 11.1000 13.7000 14.2000 14.3000 11.0000 13.8000 10.2000 8.7000 8.9000 7.5000 Taurine 235.8000 390.7000 253.9000 236.9000 408.6000 516.6000 514.5000 366.5000 554.0000 576.3000 579.5000 508.8000 512.0000 398.3000 504.8000 668.2000 566.9000 Threonine 32.5000 44.8000 30.9000 25.3000 46.6000 53.3000 71.6000 44.5000 73.0000 90.4000 81.8000 93.4000 89.1000 52.9000 65.0000 75.8000 81.8000 Trimethylamine 0.8000 1.0000 1.2000 1.2000 1.6000 2.2000 1.6000 1.1000 2.3000 1.8000 1.9000 1.6000 1.6000 1.2000 1.5000 1.8000 1.6000 Trimethylamine N-oxide 7.4000 10.0000 5.2000 6.0000 8.9000 11.0000 15.1000 7.2000 10.0000 9.5000 12.0000 8.7000 5.4000 7.9000 10.2000 12.3000 8.3000 Tryptophan 1.0000 1.2000 1.1000 1.2000 1.9000 2.0000 1.5000 1.7000 3.5000 3.7000 3.6000 3.1000 2.9000 3.4000 2.3000 4.6000 2.9000 Tyramine 0.3000 0.5000 0.4000 0.3000 0.4000 0.6000 0.4000 0.5000 0.9000 0.8000 0.8000 0.8000 0.7000 2.1000 2.2000 2.1000 1.4000 Tyrosine 2.0000 3.8000 2.6000 2.0000 3.2000 4.8000 3.9000 3.6000 8.2000 9.5000 6.3000 8.1000 7.2000 10.0000 9.2000 11.7000 9.8000 UDP-galactose 1.9000 2.2000 1.9000 1.5000 2.7000 4.1000 3.8000 1.9000 4.3000 2.6000 3.8000 3.5000 3.6000 2.6000 2.8000 3.0000 3.1000 UDP-glucose 7.7000 11.0000 7.5000 5.2000 10.9000 15.3000 13.6000 9.4000 10.3000 10.4000 11.6000 9.9000 10.0000 7.0000 6.1000 8.8000 9.0000 UDP-glucuronate 2.0000 3.5000 2.4000 2.2000 3.4000 5.0000 4.4000 2.7000 3.7000 2.8000 3.5000 3.0000 4.3000 2.4000 1.9000 3.6000 2.7000 Uracil 0.4000 0.5000 0.5000 0.4000 0.6000 0.6000 0.7000 0.7000 1.9000 1.6000 1.6000 1.5000 1.9000 2.5000 2.1000 2.1000 1.7000 Uridine 2.1000 3.9000 3.1000 3.4000 7.3000 11.4000 7.9000 5.8000 9.2000 10.1000 7.3000 7.4000 8.6000 5.2000 4.8000 7.0000 5.0000 Valine 8.9000 15.8000 10.3000 7.5000 13.8000 18.5000 14.5000 11.7000 29.5000 28.7000 29.6000 31.0000 26.0000 40.9000 37.5000 56.4000 32.1000 NMR_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant 2-Aminobutyrate 6657 2-Oxoglutarate 51 C00026 3-Hydroxybutyrate 441 C01089 3-Hydroxyisobutyrate 87 C01188 Adenosine 60961 C00212 ADP 6022 C00008 Alanine 5950 C00041 AMP 6083 C00020 Arginine 6322 C00062 Ascorbate 54670067 C00072 Aspartate 5960 C00049 ATP 5957 C00002 Betaine 247 C00719 Choline 305 C00114 Citrate 311 C00158 Creatine 586 C00300 Creatine phosphate 587 C02305 Creatinine 588 C00791 Cytidine 6175 C00475 Ethanolamine 700 C00189 Fucose 19466 C01019 Fumarate 444972 C00122 Glucose 5793 C00031 Glutamate 33032 ?-Glutamylphenylalanine 111299 Glutathione 124886 C00051 Glycerol 753 C00116 Glycine 750 C00037 GTP 6830 C00044 Histidine 6274 C00135 Hypoxanthine 790 C00262 Inosine 6021 C00294 Isobutyrate 6590 C02632 Isocitrate 1198 C00311 Lactate 107689 C00186 Leucine 6106 C00123 Lysine 5962 C00047 Malate 222656 C00149 myo-Inositol 892 C00137 Niacinamide 936 C00153 O-Phosphoethanolamine 1015 C00346 Oxypurinol 4644 C07599 Phenylalanine 6140 C00079 p-Methylhistidine Serine 5951 C00065 sn-Glycero-3-phosphocholine 657272 ß-Alanine Succinate 1110 C00042 Taurine 1123 C00245 Threonine 6288 C00188 Trimethylamine 1146 C00565 Trimethylamine N-oxide 1145 C01104 Tryptophan 6305 C00078 Tyramine 5610 C00483 Tyrosine 6057 C00082 UDP-galactose 6857410 UDP-glucose 53477679 C00029 UDP-glucuronate 17473 C00167 Uracil 1174 C00106 Uridine 6029 C00299 Valine 6287 C00183 METABOLITES_END #END