#METABOLOMICS WORKBENCH araskind_20150607_9912061_mwtab.txt DATATRACK_ID:283 STUDY_ID:ST000279 ANALYSIS_ID:AN000446 PROJECT_ID:PR000222 VERSION 1 CREATED_ON 01-15-2019 #PROJECT PR:PROJECT_TITLE Viral Effect on Metabolism PR:PROJECT_TYPE Glycolysis/TCA/Nucleotide analysis (tissue/cells) PR:PROJECT_SUMMARY Global Metabolic profiling of infection by respiratory virus PR:INSTITUTE University of Texas Medical Branch PR:DEPARTMENT Department of Pediatrics PR:LABORATORY Casola Lab PR:LAST_NAME Casola PR:FIRST_NAME Antonella PR:ADDRESS Galveston, TX PR:EMAIL yazhao1@utmb.edu PR:PHONE 409-772-2798 PR:DOI http://dx.doi.org/10.21228/M8TP4R #STUDY ST:STUDY_TITLE Viral Effect on Metabolism (part II) ST:STUDY_TYPE Glycolysis/TCA/Nucleotide analysis (tissue/cells) ST:STUDY_SUMMARY Small airway epithelial cell (SAEC) (Lonza Walkersville, Inc., MD) were grown ST:STUDY_SUMMARY according to the manufacturer's instructions in growth medium (SAGM) containing ST:STUDY_SUMMARY 0.03 mg/ml bovine pituitary extract (BPE), 0.5 µg/ml hydrocortisone, 0.5 ng/ml ST:STUDY_SUMMARY hEGF, 0.5 µg/ml epinephrine, 10 µg/ml transferrin, 5 µg/ml insulin, 0.1 ng/ml ST:STUDY_SUMMARY retinoic acid, 6.5ng/ml triiodothyronine, 50 µg/ml gentamicin and 50ng/ml ST:STUDY_SUMMARY Amphotericin-B, and 0.5 mg/ml bovine serum albumin (BSA, fatty acid free). When ST:STUDY_SUMMARY SAE grew to around 80 to 90% confluence, the cells were put into basal medium ST:STUDY_SUMMARY not supplemented with growth factors 3hours. Then the cell monolayers were ST:STUDY_SUMMARY infected with naïve hMPV at multiplicity of infection (MOI) of 3 to certain ST:STUDY_SUMMARY time. The supernatant was saved and cells were harvested and stored exactly ST:STUDY_SUMMARY according to the protocol provided by University of Michigan Metabolomics Core ST:STUDY_SUMMARY Facility for metabolomics analysis. 50mM ammonium acetate in water was used for ST:STUDY_SUMMARY rinse buffer. ST:INSTITUTE University of Michigan ST:DEPARTMENT Department of Pediatrics ST:LABORATORY Casola Lab (University of Texas Medical Branch) ST:LAST_NAME Zhao ST:FIRST_NAME Yanhua ST:ADDRESS Galveston, TX ST:EMAIL yazhao1@utmb.edu ST:PHONE 409-443-1461 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:SPECIES_GROUP Human #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - S00017199 Time:Naïve hMPV | Diseased:0hr SUBJECT_SAMPLE_FACTORS - S00017200 Time:Naïve hMPV | Diseased:0hr SUBJECT_SAMPLE_FACTORS - S00017201 Time:Naïve hMPV | Diseased:0hr SUBJECT_SAMPLE_FACTORS - S00017205 Time:Naïve hMPV | Diseased:15hrs SUBJECT_SAMPLE_FACTORS - S00017206 Time:Naïve hMPV | Diseased:15hrs SUBJECT_SAMPLE_FACTORS - S00017207 Time:Naïve hMPV | Diseased:15hrs SUBJECT_SAMPLE_FACTORS - S00017208 Time:Naïve hMPV | Diseased:24hrs SUBJECT_SAMPLE_FACTORS - S00017209 Time:Naïve hMPV | Diseased:24hrs SUBJECT_SAMPLE_FACTORS - S00017210 Time:Naïve hMPV | Diseased:24hrs SUBJECT_SAMPLE_FACTORS - S00017202 Time:Naïve hMPV | Diseased:6hrs SUBJECT_SAMPLE_FACTORS - S00017203 Time:Naïve hMPV | Diseased:6hrs SUBJECT_SAMPLE_FACTORS - S00017204 Time:Naïve hMPV | Diseased:6hrs SUBJECT_SAMPLE_FACTORS - S00017187 Time:RSV | Diseased:0hr SUBJECT_SAMPLE_FACTORS - S00017188 Time:RSV | Diseased:0hr SUBJECT_SAMPLE_FACTORS - S00017189 Time:RSV | Diseased:0hr SUBJECT_SAMPLE_FACTORS - S00017193 Time:RSV | Diseased:15hrs SUBJECT_SAMPLE_FACTORS - S00017194 Time:RSV | Diseased:15hrs SUBJECT_SAMPLE_FACTORS - S00017195 Time:RSV | Diseased:15hrs SUBJECT_SAMPLE_FACTORS - S00017196 Time:RSV | Diseased:24hrs SUBJECT_SAMPLE_FACTORS - S00017197 Time:RSV | Diseased:24hrs SUBJECT_SAMPLE_FACTORS - S00017198 Time:RSV | Diseased:24hrs SUBJECT_SAMPLE_FACTORS - S00017190 Time:RSV | Diseased:6hrs SUBJECT_SAMPLE_FACTORS - S00017191 Time:RSV | Diseased:6hrs SUBJECT_SAMPLE_FACTORS - S00017192 Time:RSV | Diseased:6hrs #COLLECTION CO:SAMPLE_TYPE Cells, Cultured CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_PROTOCOL_FILENAME Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx SP:SAMPLEPREP_SUMMARY - #CHROMATOGRAPHY CH:METHODS_ID AQM011 CH:METHODS_FILENAME ALPHA_KETO_ACIDS-FULL.M.zip CH:INSTRUMENT_NAME Agilent 7890A CH:COLUMN_NAME Agilent DB5-MS (30m × 0.25mm, 0.25um) CH:CHROMATOGRAPHY_TYPE GC #ANALYSIS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ANALYSIS_TYPE MS AN:ACQUISITION_PARAMETERS_FILE ALPHA_KETO_ACIDS-FULL.M #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/µg protein MS_METABOLITE_DATA_START Samples S00017199 S00017200 S00017201 S00017205 S00017206 S00017207 S00017208 S00017209 S00017210 S00017202 S00017203 S00017204 S00017187 S00017188 S00017189 S00017193 S00017194 S00017195 S00017196 S00017197 S00017198 S00017190 S00017191 S00017192 Factors Time:Naïve hMPV | Diseased:0hr Time:Naïve hMPV | Diseased:0hr Time:Naïve hMPV | Diseased:0hr Time:Naïve hMPV | Diseased:15hrs Time:Naïve hMPV | Diseased:15hrs Time:Naïve hMPV | Diseased:15hrs Time:Naïve hMPV | Diseased:24hrs Time:Naïve hMPV | Diseased:24hrs Time:Naïve hMPV | Diseased:24hrs Time:Naïve hMPV | Diseased:6hrs Time:Naïve hMPV | Diseased:6hrs Time:Naïve hMPV | Diseased:6hrs Time:RSV | Diseased:0hr Time:RSV | Diseased:0hr Time:RSV | Diseased:0hr Time:RSV | Diseased:15hrs Time:RSV | Diseased:15hrs Time:RSV | Diseased:15hrs Time:RSV | Diseased:24hrs Time:RSV | Diseased:24hrs Time:RSV | Diseased:24hrs Time:RSV | Diseased:6hrs Time:RSV | Diseased:6hrs Time:RSV | Diseased:6hrs alpha-Ketoglutarate 32.6500 52.4178 44.7558 40.7813 43.8859 39.4141 51.7277 56.6941 62.0791 34.4216 45.6329 41.3521 31.2507 11.0836 27.5194 27.8848 29.7566 31.9329 28.6412 31.5092 62.9630 49.5530 41.2806 Aspartate 73.7953 87.0211 86.3011 49.2223 52.7632 53.8199 50.4948 59.1769 60.3232 39.2418 51.1835 50.2460 62.1800 22.5009 52.9602 35.1567 35.5109 47.6875 40.3097 41.6907 76.1404 58.2812 45.9066 fumarate 25.5208 29.5311 27.8301 35.9359 35.9513 36.4617 43.6704 41.1650 55.4450 27.6999 29.9889 27.8468 24.5951 8.1163 24.6142 31.4305 31.1751 30.4861 30.5519 30.6256 42.0009 30.9502 26.5947 Glutamate 622.2134 812.8791 703.0015 952.0996 941.6904 1018.6092 630.6445 932.6074 926.6618 711.1092 865.9719 775.7435 582.2829 179.3840 414.2969 1029.7894 1017.4643 1404.1148 1319.9344 1367.7723 976.6936 732.0595 627.0329 glutamine 9610.5340 11036.1690 8391.8905 9565.4543 11527.4010 11566.7740 13202.4590 17905.1390 16597.2960 5283.7992 7219.7367 7073.6992 7087.3786 2580.9220 5535.9107 7102.9071 6448.4195 14280.6620 11418.6560 14251.5200 8362.6955 7671.2637 5752.1519 Lactate 1226.8116 1154.3111 1088.7150 9558.6964 11747.7510 10819.8770 16091.5630 21880.6420 21472.4130 1787.1996 1630.5334 1317.7483 757.7682 355.2299 783.4521 9016.8693 9533.0856 12403.2630 14430.5830 19211.5900 1775.7993 1436.6286 1238.3704 Oxaloacetate pyruvate 193.1402 189.6068 181.9528 162.1089 220.4977 184.1804 267.3724 316.4268 355.1142 98.4989 109.9000 100.6977 113.3102 55.8973 111.1645 57.4663 65.2732 90.7803 47.6361 46.2901 125.9344 116.2615 93.6529 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant alpha-Ketoglutarate 51 C00026 AKG UM_Target_ID Aspartate 5960 C00049 ASP UM_Target_ID fumarate 444972 C00122 FUM UM_Target_ID Glutamate 611 GLU UM_Target_ID glutamine 5961 C00064 GLN UM_Target_ID Lactate 107689 C00186 LAC UM_Target_ID Oxaloacetate 970 C00036 OAA UM_Target_ID pyruvate 1060 C00022 PYR UM_Target_ID METABOLITES_END #END