#METABOLOMICS WORKBENCH araskind_20151217_9109411_mwtab.txt DATATRACK_ID:463 efahy_20151228_123430 STUDY_ID:ST000300 ANALYSIS_ID:AN000478 PROJECT_ID:PR000241 VERSION 1 CREATED_ON December 28, 2015, 12:34 pm #PROJECT PR:PROJECT_TITLE Identifiying Colonization Factors of Campylobacter jejuni PR:PROJECT_SUMMARY Using next-generation approaches to identify both bacterial and host factors PR:PROJECT_SUMMARY that influence colonization of the chicken gastrointestinal tract by C. jejuni PR:INSTITUTE University of Michigan PR:DEPARTMENT Microbiology and Immunology PR:LABORATORY DiRita Lab PR:LAST_NAME DiRita PR:FIRST_NAME Victor PR:ADDRESS Ann Arbor, MI PR:EMAIL vdirita@umich.edu PR:PHONE 734-936-3804 #STUDY ST:STUDY_TITLE Chicken/C. jejuni Bile Acid Study ST:STUDY_SUMMARY Cecal samples were isolated directly from the ceca of dissected chickens that ST:STUDY_SUMMARY were either experimentally infected with C. jejuni DRH212 or mock-infected with ST:STUDY_SUMMARY PBS. Cecal samples were re-suspended in Life Technologies 1X PBS based on weight ST:STUDY_SUMMARY of sample (1 ml/100 mg = 10-1 dilution) and stored at -80C. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 4 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Gallus gallus SU:TAXONOMY_ID 9031 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0011938 S00017774 C. jejuni Infection:yes SUBJECT_SAMPLE_FACTORS SU0011938 S00017775 C. jejuni Infection:yes SUBJECT_SAMPLE_FACTORS SU0011938 S00017776 C. jejuni Infection:no SUBJECT_SAMPLE_FACTORS SU0011938 S00017777 C. jejuni Infection:no #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Cecum #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Bile Acids-20150305.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1260 CH:COLUMN_NAME Waters Acquity BEH C18 (50 x 2mm, 1.7um) CH:METHODS_FILENAME 2014-07-30-Bile_acid-full-BEH-C18-I.m #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE Bile Acids-20150305.docx #MS MS:MS_COMMENTS - MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:INSTRUMENT_NAME Agilent 6410 QQQ MS:INSTRUMENT_NAME Agilent 6410 QQQ #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples S00017774 S00017775 S00017776 S00017777 Factors C. jejuni Infection:yes C. jejuni Infection:yes C. jejuni Infection:no C. jejuni Infection:no a-Muricholic acid 0 56.81936 0 0 b-Muricholic acid 34.2 33.8 74.8 40.1 Cholic acid 756.1 2153 1722 1311 Chenodeoxycholic acid 0 0 0 0 Deoxycholic acid 63.7 1980 241 0 Glychocholic acid 0 0 0 0 Glycochenodeoxycholic acid 0 0 0 0 Glycodeoxycholic acid 44.1 39.1 39.3 41.9 Glycohyodeoxycholic acid 0 0 0 0 Glycolithocholic acid 0 0 0 0 Glycoursodeoxycholic acid 0 0 0 0 g-Muricholic acid (hyocholic acid) 306.1 256.3 465.2 792.1 Hyodeoxycholic acid 0 0 0 0 Taurocholic acid 1009.8 356.2 119 150.4 Taurochenodeoxycholic acid 437.8 466.4 523.9 123 Taurodeoxycholic acid 38.8 12.1 9.4 8.5 Tauro-hyodeoxycholic acid 86.1 32.4 32.2 33.2 Taurolithocolic acid 162.5 272.2 163.1 153.5 Tauroursodeoxycholic acid 87.6 34.1 34 34.1 Ursodeoxycholic acid 0 0 0 0 w-Muricholic acid 115 152.6 268.7 596.2 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Local ID PubChem CID a-Muricholic acid aMCA 53477700 b-Muricholic acid bMCA 5283853 Cholic acid CA 221493 Chenodeoxycholic acid CDCA 10133 Deoxycholic acid DCA 6742 Glychocholic acid GCA 10140 Glycochenodeoxycholic acid GCDCA 9548796 Glycodeoxycholic acid GDCA 18602029 Glycohyodeoxycholic acid GHDCA 114611 Glycolithocholic acid GLCA 115245 Glycoursodeoxycholic acid GUDCA 12310288 g-Muricholic acid (hyocholic acid) HCA 92805 Hyodeoxycholic acid HDCA 16219478 Taurocholic acid TCA 6675 Taurochenodeoxycholic acid TCDCA 54608447 Taurodeoxycholic acid TDCA 10594 Tauro-hyodeoxycholic acid THDCA 9848818 Taurolithocolic acid TLCA 10595 Tauroursodeoxycholic acid TUDCA 9848818 Ursodeoxycholic acid UDCA 54285959 w-Muricholic acid wMCA 5283851 METABOLITES_END #END