#METABOLOMICS WORKBENCH amitch_20160114_9407421_mwtab.txt DATATRACK_ID:481 kenanazam_20160121_111839 STUDY_ID:ST000337 ANALYSIS_ID:AN000548 PROJECT_ID:PR000269
VERSION             	1
CREATED_ON             	January 21, 2016, 11:48 am
#PROJECT
PR:PROJECT_TITLE                 	Metabolomics approach to allograft assessment in liver transplantation
PR:PROJECT_TYPE                  	Retrospective analysis of biobank samples
PR:PROJECT_SUMMARY               	Organ shortage is the primary limiting factor in liver transplantation and has
PR:PROJECT_SUMMARY               	led to expanded utilization of livers from marginal donors. Assessment of liver
PR:PROJECT_SUMMARY               	allograft quality is currently based on clinical factors such as donor age,
PR:PROJECT_SUMMARY               	cause of death, liver function, routine clinical laboratory values and extent of
PR:PROJECT_SUMMARY               	graft steatosis (fat content). Expanded utilization necessitates a more detailed
PR:PROJECT_SUMMARY               	assessment of liver allograft quality to guide decisions regarding suitability
PR:PROJECT_SUMMARY               	for transplantation. Using a metabolomics approach, we plan to capture molecular
PR:PROJECT_SUMMARY               	snapshots of the physiologic state of the graft and to elucidate biomarkers of
PR:PROJECT_SUMMARY               	allograft quality and graft function post-transplant.
PR:INSTITUTE                     	Ochsner Multi-Organ Transplant Institute / SECIM
PR:DEPARTMENT                    	Abdominal Organ Transplantation
PR:LABORATORY                    	-
PR:LAST_NAME                     	Seal
PR:FIRST_NAME                    	John
PR:ADDRESS                       	1514 Jefferson Highway, New Orleans, LA 70121
PR:EMAIL                         	John.Seal@ochsner.org
PR:PHONE                         	504-232-4253
PR:FUNDING_SOURCE                	SECIM Pilot and Feasibility Award
#STUDY
ST:STUDY_TITLE                   	Metabolomics Approach to Allograft Assessment in Liver Transplantation
ST:STUDY_TYPE                    	Retrospective analysis of biobanked liver tissue from organ donors
ST:STUDY_SUMMARY                 	This pilot study is designed to apply several types of metabolomic analysis for
ST:STUDY_SUMMARY                 	liver allograft assessment with the aim of identifying candidate biomarkers for
ST:STUDY_SUMMARY                 	allograft function and to develop a methodology that could be applied to larger
ST:STUDY_SUMMARY                 	scale studies. The three main categories of metabolomic analysis are reflected
ST:STUDY_SUMMARY                 	in each of the specific aims, including a targeted profiling of central carbon
ST:STUDY_SUMMARY                 	metabolism, open lipidomic and metabolomic profiling for hypothesis generation
ST:STUDY_SUMMARY                 	and MALDI-IMS for tissue-based spatial analysis. Each of these approaches offers
ST:STUDY_SUMMARY                 	potential benefits that could be optimized in a protocol for larger scale
ST:STUDY_SUMMARY                 	studies depending the results of the pilot investigations.
ST:INSTITUTE                     	Ochsner Multi-Organ Transplant Institute
ST:DEPARTMENT                    	SECIM
ST:LABORATORY                    	Abdominal Organ Transplantation
ST:LAST_NAME                     	Seal
ST:FIRST_NAME                    	John
ST:ADDRESS                       	1514 Jefferson Highway, New Orleans, LA 70121
ST:EMAIL                         	John.Seal@ochsner.org
ST:PHONE                         	504-232-4253
ST:NUM_GROUPS                    	3
ST:TOTAL_SUBJECTS                	Targeted analysis and open profiling: Control group - standard criteria donor
ST:TOTAL_SUBJECTS                	(N=15); Experimental group - donation-after-cardiac-death donors (N=10).
ST:TOTAL_SUBJECTS                	MALDI-IMS - standard criteria donor (N=10)
#SUBJECT
SU:SUBJECT_TYPE                  	Human tissue (Decendent)
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:GENOTYPE_STRAIN               	Not applicable
SU:AGE_OR_AGE_RANGE              	Variable
SU:WEIGHT_OR_WEIGHT_RANGE        	Variable
SU:HEIGHT_OR_HEIGHT_RANGE        	Variable
SU:GENDER                        	Variable
SU:HUMAN_RACE                    	Variable
SU:HUMAN_ETHNICITY               	Variable
SU:HUMAN_TRIAL_TYPE              	Not applicable
SU:HUMAN_LIFESTYLE_FACTORS       	Not applicable
SU:HUMAN_MEDICATIONS             	Not applicable
SU:HUMAN_PRESCRIPTION_OTC        	Not applicable
SU:HUMAN_SMOKING_STATUS          	Not applicable
SU:HUMAN_ALCOHOL_DRUG_USE        	Not applicable
SU:HUMAN_NUTRITION               	Not applicable
SU:HUMAN_INCLUSION_CRITERIA      	Deceased organ donor with consent for use of tissue for research
SU:HUMAN_EXCLUSION_CRITERIA      	None
SU:ANIMAL_ANIMAL_SUPPLIER        	in house/in house
SU:SUBJECT_COMMENTS              	Liver biopsy is routinely performed on livers used for solid organ
SU:SUBJECT_COMMENTS              	transplantation. The tissue is from decedent subjects. Consent for use of tissue
SU:SUBJECT_COMMENTS              	is obtained at the time of organ donation by the local organ procurement
SU:SUBJECT_COMMENTS              	organization.
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	114291	DONOR CAUSE OF DEATH:Anoxia	RECOVERY DATE=1/20/2015; OPO=AROR; ABO=A; AGE (years)=46; RACE=CAU; SEX=F; DONOR HEIGHT (cm)=163; DONOR WEIGHT (kg)=74.4; Hep C Positive Donor=NO; Cold Ischemia (min)=327; Cold Ischemia (Hrs)=5.45; DCD DONOR=YES; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	116707	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=2/7/2015; OPO=LAOP; ABO=A; AGE (years)=52; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=175.26; DONOR WEIGHT (kg)=96.79; Hep C Positive Donor=NO; Cold Ischemia (min)=278; Cold Ischemia (Hrs)=4.63; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	115165	DONOR CAUSE OF DEATH:Anoxia	RECOVERY DATE=4/1/2015; OPO=TXSB; ABO=O; AGE (years)=59; RACE=CAU; SEX=F; DONOR HEIGHT (cm)=165; DONOR WEIGHT (kg)=63; Hep C Positive Donor=NO; Cold Ischemia (min)=323; Cold Ischemia (Hrs)=5.38; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	115333	DONOR CAUSE OF DEATH:Cerebrovascular/Stroke	RECOVERY DATE=4/10/2015; OPO=PADV; ABO=B; AGE (years)=44; RACE=BLK; SEX=M; DONOR HEIGHT (cm)=180.34; DONOR WEIGHT (kg)=59; Hep C Positive Donor=NO; Cold Ischemia (min)=454; Cold Ischemia (Hrs)=7.57; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	115342	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=4/10/2015; OPO=LAOP; ABO=B; AGE (years)=46; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=180.34; DONOR WEIGHT (kg)=103.78; Hep C Positive Donor=NO; Cold Ischemia (min)=216; Cold Ischemia (Hrs)=3.60; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	117751	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=6/25/2015; OPO=LAOP; ABO=O; AGE (years)=48; RACE=BLK; SEX=M; DONOR HEIGHT (cm)=182.9; DONOR WEIGHT (kg)=105.9; Hep C Positive Donor=NO; Cold Ischemia (min)=228; Cold Ischemia (Hrs)=3.80; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	121063	DONOR CAUSE OF DEATH:Cerebrovascular/Stroke	RECOVERY DATE=8/26/2015; OPO=LAOP; ABO=B; AGE (years)=48; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=154.94; DONOR WEIGHT (kg)=119.15; Hep C Positive Donor=NO; Cold Ischemia (min)=207; Cold Ischemia (Hrs)=3.45; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	120237	DONOR CAUSE OF DEATH:Anoxia	RECOVERY DATE=8/5/2015; OPO=LAOP; ABO=O; AGE (years)=40; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=177.8; DONOR WEIGHT (kg)=82.8; Hep C Positive Donor=NO; Cold Ischemia (min)=320; Cold Ischemia (Hrs)=5.33; DCD DONOR=YES; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	120240	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=8/6/2015; OPO=TNDS; ABO=AB; AGE (years)=27; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=183; DONOR WEIGHT (kg)=93.3; Hep C Positive Donor=NO; Cold Ischemia (min)=293; Cold Ischemia (Hrs)=4.88; DCD DONOR=YES; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	120257	DONOR CAUSE OF DEATH:Anoxia	RECOVERY DATE=8/15/2015; OPO=ALOB; ABO=O; AGE (years)=28; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=183; DONOR WEIGHT (kg)=78; Hep C Positive Donor=YES; Cold Ischemia (min)=226; Cold Ischemia (Hrs)=3.77; DCD DONOR=YES; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	121095	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=9/22/2015; OPO=LAOP; ABO=O; AGE (years)=23; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=170.18; DONOR WEIGHT (kg)=61.37; Hep C Positive Donor=NO; Cold Ischemia (min)=171; Cold Ischemia (Hrs)=2.85; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	114300	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=1/31/2015; OPO=LAOP; ABO=A; AGE (years)=25; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=182.88; DONOR WEIGHT (kg)=66.22; Hep C Positive Donor=NO; Cold Ischemia (min)=377; Cold Ischemia (Hrs)=6.28; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	116736	DONOR CAUSE OF DEATH:Anoxia	RECOVERY DATE=4/11/2015; OPO=LAOP; ABO=B; AGE (years)=25; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=139.7; DONOR WEIGHT (kg)=84.14; Hep C Positive Donor=NO; Cold Ischemia (min)=281; Cold Ischemia (Hrs)=4.68; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	121087	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=9/18/2015; OPO=LAOP; ABO=O; AGE (years)=26; RACE=BLK; SEX=M; DONOR HEIGHT (cm)=175.26; DONOR WEIGHT (kg)=74.84; Hep C Positive Donor=NO; Cold Ischemia (min)=366; Cold Ischemia (Hrs)=6.10; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	117323	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=6/8/2015; OPO=LAOP; ABO=O; AGE (years)=27; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=177.8; DONOR WEIGHT (kg)=67.8; Hep C Positive Donor=NO; Cold Ischemia (min)=262; Cold Ischemia (Hrs)=4.37; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	121091	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=9/20/2015; OPO=LAOP; ABO=O; AGE (years)=31; RACE=CAU; SEX=F; DONOR HEIGHT (cm)=172.72; DONOR WEIGHT (kg)=77.65; Hep C Positive Donor=NO; Cold Ischemia (min)=414; Cold Ischemia (Hrs)=6.90; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	121089	DONOR CAUSE OF DEATH:Anoxia	RECOVERY DATE=9/19/2015; OPO=LAOP; ABO=O; AGE (years)=34; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=182.88; DONOR WEIGHT (kg)=95; Hep C Positive Donor=NO; Cold Ischemia (min)=46; Cold Ischemia (Hrs)=0.77; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	116709	DONOR CAUSE OF DEATH:Trauma	RECOVERY DATE=2/17/2015; OPO=LAOP; ABO=O; AGE (years)=35; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=182.88; DONOR WEIGHT (kg)=72.57; Hep C Positive Donor=NO; Cold Ischemia (min)=367; Cold Ischemia (Hrs)=6.12; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	114647	DONOR CAUSE OF DEATH:Cerebrovascular/Stroke	RECOVERY DATE=2/12/2015; OPO=LAOP; ABO=O; AGE (years)=39; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=170.18; DONOR WEIGHT (kg)=80.83; Hep C Positive Donor=NO; Cold Ischemia (min)=156; Cold Ischemia (Hrs)=2.60; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	118601	DONOR CAUSE OF DEATH:Anoxia	RECOVERY DATE=7/19/2015; OPO=LAOP; ABO=A1; AGE (years)=39; RACE=CAU; SEX=F; DONOR HEIGHT (cm)=154.9; DONOR WEIGHT (kg)=62.5; Hep C Positive Donor=NO; Cold Ischemia (min)=236; Cold Ischemia (Hrs)=3.93; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	121079	DONOR CAUSE OF DEATH:Anoxia	RECOVERY DATE=9/8/2015; OPO=LAOP; ABO=O; AGE (years)=40; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=160; DONOR WEIGHT (kg)=67.2; Hep C Positive Donor=NO; Cold Ischemia (min)=338; Cold Ischemia (Hrs)=5.63; DCD DONOR=NO; PHS Increased Risk Donor=YES; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	121364	DONOR CAUSE OF DEATH:CVA	RECOVERY DATE=10/7/2015; OPO=LAOP; ABO=O; AGE (years)=43; RACE=CAU; SEX=M; DONOR HEIGHT (cm)=172.72; DONOR WEIGHT (kg)=82.9; Hep C Positive Donor=NO; Cold Ischemia (min)=54; Cold Ischemia (Hrs)=0.90; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	121358	DONOR CAUSE OF DEATH:CVA	RECOVERY DATE=10/4/2015; OPO=LAOP; ABO=O; AGE (years)=44; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=162.6; DONOR WEIGHT (kg)=53.9; Hep C Positive Donor=NO; Cold Ischemia (min)=246; Cold Ischemia (Hrs)=4.10; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	121356	DONOR CAUSE OF DEATH:CVA	RECOVERY DATE=10/3/2015; OPO=LAOP; ABO=O; AGE (years)=49; RACE=BLK; SEX=M; DONOR HEIGHT (cm)=167.6; DONOR WEIGHT (kg)=52.4; Hep C Positive Donor=NO; Cold Ischemia (min)=336; Cold Ischemia (Hrs)=5.60; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
SUBJECT_SAMPLE_FACTORS           	-	115347	DONOR CAUSE OF DEATH:Anoxia	RECOVERY DATE=4/23/2015; OPO=LAOP; ABO=B; AGE (years)=49; RACE=BLK; SEX=F; DONOR HEIGHT (cm)=162.56; DONOR WEIGHT (kg)=73.84; Hep C Positive Donor=NO; Cold Ischemia (min)=280; Cold Ischemia (Hrs)=4.67; DCD DONOR=NO; PHS Increased Risk Donor=NO; Hepatits B Core Ab +=NO
#COLLECTION
CO:COLLECTION_SUMMARY            	A wedge biopsy of the right lobe of the liver is routinely obtained prior to
CO:COLLECTION_SUMMARY            	transplantation. The sample is immediately flash frozen in the operating room
CO:COLLECTION_SUMMARY            	and subsequently transferred to Ochsner Biobank for sample storage.
CO:SAMPLE_TYPE                   	Liver Tissue
CO:COLLECTION_METHOD             	Open biopsy during cold storage preservation of organ for transplant
CO:COLLECTION_LOCATION           	Operating room of liver transplant during
CO:COLLECTION_FREQUENCY          	Single sample prior to transplant
CO:COLLECTION_DURATION           	Not applicable
CO:COLLECTION_TIME               	Not applicable
CO:VOLUMEORAMOUNT_COLLECTED      	~50mg
CO:STORAGE_CONDITIONS            	-180 degree C, liquid nitrogen vapor phase
CO:COLLECTION_VIALS              	ThermoScientific CryoTube vials, 1.8mL
CO:STORAGE_VIALS                 	ThermoScientific CryoTube vials, 1.8mL
CO:COLLECTION_TUBE_TEMP          	-70 degree liguid nitrogen flash freezing
CO:ADDITIVES                     	None
CO:BLOOD_SERUM_OR_PLASMA         	N/A
CO:TISSUE_CELL_IDENTIFICATION    	N/A
CO:TISSUE_CELL_QUANTITY_TAKEN    	N/A
#TREATMENT
TR:TREATMENT_SUMMARY             	Not Applicable
TR:TREATMENT_PROTOCOL_ID         	Not Applicable
TR:TREATMENT_PROTOCOL_FILENAME   	Not Applicable
TR:TREATMENT_PROTOCOL_COMMENTS   	Not Applicable
TR:TREATMENT                     	Not Applicable
TR:TREATMENT_COMPOUND            	Not Applicable
TR:TREATMENT_ROUTE               	Not Applicable
TR:TREATMENT_DOSE                	Not Applicable
TR:TREATMENT_DOSEVOLUME          	Not Applicable
TR:TREATMENT_DOSEDURATION        	Not Applicable
TR:TREATMENT_VEHICLE             	Not Applicable
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Amino acid assay|CoA assay|NAD-ATP assay|NADH assay|Organic acid assay
SP:SAMPLEPREP_PROTOCOL_ID        	SB_AA_Assay.pdf;SB_CoA_Assay.pdf;SB_NAD-ATP_Assay_Agilent.pdf;SB_NADH_Assay_Agilent.pdf;SB_OA_Assay.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Organic acid assay
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
MS:INSTRUMENT_NAME               	Thermo Quantiva QQQ
CH:COLUMN_NAME                   	Waters Acquity BEH C18 (100 x 2mm, 1.7um)
CH:METHODS_FILENAME              	SB_OA_Assay.pdf
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Quantiva QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Organic acid assay-ESI
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	nmol/mg dry powder
MS_METABOLITE_DATA_START
Samples	114291	116707	115165	115333	115342	117751	121063	120237	120240	120257	121095	114300	116736	121087	117323	121091	121089	116709	114647	118601	121079	121364	121358	121356	115347
Factors	DONOR CAUSE OF DEATH:Anoxia	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Anoxia	DONOR CAUSE OF DEATH:Cerebrovascular/Stroke	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Cerebrovascular/Stroke	DONOR CAUSE OF DEATH:Anoxia	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Anoxia	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Anoxia	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Anoxia	DONOR CAUSE OF DEATH:Trauma	DONOR CAUSE OF DEATH:Cerebrovascular/Stroke	DONOR CAUSE OF DEATH:Anoxia	DONOR CAUSE OF DEATH:Anoxia	DONOR CAUSE OF DEATH:CVA	DONOR CAUSE OF DEATH:CVA	DONOR CAUSE OF DEATH:CVA	DONOR CAUSE OF DEATH:Anoxia
Lactate	16.78	33.64	19.91	27.75	19.34	28.49	20.48	19.60	33.61	26.97	19.48	10.83	20.35	17.50	34.76	18.83	16.57	38.18	8.44	15.58	16.57	30.11	6.80		12.64
Pyruvate	0.09	0.36		0.36		0.35				0.43	0.27	0.17		0.23	0.17	0.19		0.44			0.28	0.33			
_3_HBA												0.79			0.62			0.63							
Succinate	1.44	1.56	0.75	3.61	1.08	1.69	0.81	0.72	2.18	1.98	1.22	1.88	2.21	1.40	2.11	1.38	1.02	3.84	0.29	0.81	1.19	1.86	1.23		1.31
Fumarate	0.25	0.38	0.20	0.38	0.19	0.28	0.21	0.19	0.34	0.21	0.30	0.41	0.25	0.27	0.48	0.40	0.35	0.44	0.09	0.12	0.30	0.26	0.43		0.28
Malate	1.97	2.57	1.63	3.47	1.36	2.62	2.13	1.62	3.32	2.09	2.16	3.06	1.96	2.02	3.94	2.66	2.39	3.41	0.57	1.08	2.17	1.83	3.50		2.47
a-KG	0.01	0.03		0.08	0.01	0.03	0.04		0.05	0.09	0.03	0.03	0.02	0.03	0.02	0.02	0.05	0.03	0.02	0.03	0.04	0.05	0.03		0.06
Citrate	0.35			0.09	0.08	0.12	0.20	0.20	N.D.	0.22	0.15	0.25	0.15	0.16	0.10	0.09	0.26	0.15	0.16	0.11	0.13	0.07	0.23		0.21
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	retention index	quantified m/z	PubChem ID	KEGG ID
Lactate	3.98	196.1	107689
Pyruvate	6.68	299.1	1060
_3_HBA	4.21	210.1	441
Succinate	5.44	329.1	1110
Fumarate	5.59	327.1	444972
Malate	5.28	345.1	525
a-KG	6.59	462.2	164533
Citrate	5.95	508.25	311
METABOLITES_END
#END