#METABOLOMICS WORKBENCH araskind_20160414_082215 DATATRACK_ID:588 STUDY_ID:ST000376 ANALYSIS_ID:AN000610 PROJECT_ID:PR000296 VERSION 1 CREATED_ON April 19, 2016, 3:19 pm #PROJECT PR:PROJECT_TITLE Role of kidney acetylcholine in protection from kidney injury PR:PROJECT_SUMMARY ChAT was induced in a model of kidney inflammation to determine its role in PR:PROJECT_SUMMARY kidney protection PR:INSTITUTE University of Colorado, Denver PR:DEPARTMENT Medicine PR:LABORATORY Garcia Lab PR:LAST_NAME Garcia PR:FIRST_NAME Gabriela PR:ADDRESS Denver, CO PR:EMAIL Gabriela.garcia@ucdenver.edu PR:PHONE 303-724-4897 #STUDY ST:STUDY_TITLE Kidney choline acetyltransferase (ChAT)-3 ST:STUDY_TYPE Regular ST:STUDY_SUMMARY We use ChAT(BAC)-EGFP mice, which express enhanced green fluorescent protein ST:STUDY_SUMMARY (EGFP) under the control of transcriptional regulatory elements for choline ST:STUDY_SUMMARY acetyltransferase (ChAT), the sole enzyme that catalyzes the biosynthesis of ST:STUDY_SUMMARY acetylcholine (ACh). These mice were treated with inducers of ChAT. Kidney were ST:STUDY_SUMMARY rapidly removed, frozen on liquid nitrogen and stored at -80C ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 7 ST:TOTAL_SUBJECTS 25 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0012166 S00018085 Treated:Treated | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018086 Treated:Treated | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018087 Treated:Treated | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018088 Treated:Treated | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018089 Treated:Treated | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018090 Treated:Treated | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018091 Treated:Treated | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018092 Treated:Treated | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018093 Treated:Treated | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018094 Treated:Vehicle | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018095 Treated:Treated | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018096 Treated:Treated | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018097 Treated:Vehicle | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018098 Treated:Treated | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018099 Treated:Treated | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018100 Treated:Treated | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018101 Treated:Control | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018102 Treated:Control | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012167 S00018103 Treated:Control | Gender:Female | Strain:WT SUBJECT_SAMPLE_FACTORS SU0012167 S00018104 Treated:Control | Gender:Female | Strain:WT SUBJECT_SAMPLE_FACTORS SU0012166 S00018105 Treated:Treated | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018106 Treated:Treated | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018107 Treated:Treated | Gender:Female | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012166 S00018108 Treated:Control | Gender:Male | Strain:ChAT SUBJECT_SAMPLE_FACTORS SU0012167 S00018109 Treated:Control | Gender:Male | Strain:WT #COLLECTION CO:COLLECTION_SUMMARY none CO:SAMPLE_TYPE Kidney #TREATMENT TR:TREATMENT_SUMMARY none #SAMPLEPREP SP:SAMPLEPREP_SUMMARY none SP:SAMPLEPREP_PROTOCOL_FILENAME 2014-10-07-Neurotransmitter.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE None CH:INSTRUMENT_NAME Unspecified CH:COLUMN_NAME Cogent Diamond Hydride CH:METHODS_FILENAME QM009_2mm_DH_Neurotransmitter_MRM-ESP-1.m.zip CH:METHODS_ID DQM0150 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6410A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg sample MS_METABOLITE_DATA_START Samples S00018085 S00018086 S00018087 S00018088 S00018089 S00018090 S00018091 S00018092 S00018093 S00018094 S00018095 S00018096 S00018097 S00018098 S00018099 S00018100 S00018101 S00018102 S00018103 S00018104 S00018105 S00018106 S00018107 S00018108 Factors Treated:Treated | Gender:Female | Strain:ChAT Treated:Treated | Gender:Female | Strain:ChAT Treated:Treated | Gender:Female | Strain:ChAT Treated:Treated | Gender:Female | Strain:ChAT Treated:Treated | Gender:Female | Strain:ChAT Treated:Treated | Gender:Female | Strain:ChAT Treated:Treated | Gender:Female | Strain:ChAT Treated:Treated | Gender:Male | Strain:ChAT Treated:Treated | Gender:Male | Strain:ChAT Treated:Vehicle | Gender:Male | Strain:ChAT Treated:Treated | Gender:Male | Strain:ChAT Treated:Treated | Gender:Male | Strain:ChAT Treated:Vehicle | Gender:Male | Strain:ChAT Treated:Treated | Gender:Male | Strain:ChAT Treated:Treated | Gender:Male | Strain:ChAT Treated:Treated | Gender:Male | Strain:ChAT Treated:Control | Gender:Female | Strain:ChAT Treated:Control | Gender:Female | Strain:ChAT Treated:Control | Gender:Female | Strain:WT Treated:Control | Gender:Female | Strain:WT Treated:Treated | Gender:Male | Strain:ChAT Treated:Treated | Gender:Female | Strain:ChAT Treated:Treated | Gender:Female | Strain:ChAT Treated:Control | Gender:Male | Strain:ChAT 5-Hydroxyindoleacetic acid 54.35 60.32 11.86 137.95 41.64 15.35 32.93 78.95 17.52 21.21 39.39 22.83 37.21 87.97 117.58 13.79 23.65 8.53 21.56 16.70 24.86 158.95 10.69 229.03 Adenosine 215.39 177.95 211.85 208.78 227.71 24.84 148.61 201.35 169.80 154.60 227.84 158.49 433.38 383.69 350.31 283.57 290.94 175.44 232.07 161.29 243.21 358.18 222.07 322.47 Serotonin 1.59 1.45 1.51 1.51 1.50 1.30 1.58 1.54 1.64 0.00 1.53 1.39 1.62 1.45 1.43 1.33 1.32 1.28 1.30 1.32 1.51 1.54 1.61 1.65 3-Methoxytyramine 0.76 0.77 0.77 0.76 0.76 0.79 0.76 0.78 0.82 0.00 0.78 0.80 0.77 0.77 0.77 0.77 0.76 0.76 0.76 0.76 0.78 0.76 0.78 0.77 Acetylcholine 2.77 2.48 6.08 1.93 2.95 5.77 3.55 3.26 2.72 1.36 15.67 2.01 2.35 19.49 10.77 2.73 2.96 1.50 0.00 0.00 2.47 3.36 13.08 6.68 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID 5-Hydroxyindoleacetic acid 1826 Adenosine 60961 Serotonin 5202 3-Methoxytyramine 1669 Acetylcholine 187 METABOLITES_END #END