#METABOLOMICS WORKBENCH araskind_20160414_082440 DATATRACK_ID:589 STUDY_ID:ST000377 ANALYSIS_ID:AN000611 PROJECT_ID:PR000296 VERSION 1 CREATED_ON April 19, 2016, 4:06 pm #PROJECT PR:PROJECT_TITLE Role of kidney acetylcholine in protection from kidney injury PR:PROJECT_SUMMARY ChAT was induced in a model of kidney inflammation to determine its role in PR:PROJECT_SUMMARY kidney protection PR:INSTITUTE University of Colorado, Denver PR:DEPARTMENT Medicine PR:LABORATORY Garcia Lab PR:LAST_NAME Garcia PR:FIRST_NAME Gabriela PR:ADDRESS Denver, CO PR:EMAIL Gabriela.garcia@ucdenver.edu PR:PHONE 303-724-4897 #STUDY ST:STUDY_TITLE Kidney choline acetyltransferase (ChAT)-(part II) ST:STUDY_TYPE Regular ST:STUDY_SUMMARY Rats were treated with donepezil (DPZ) after the induction of a model of ST:STUDY_SUMMARY glomerulonephritis (GN) to increase kidney ACh levels. Rats were euthanized at ST:STUDY_SUMMARY day 25 after the induction of the disease. Kidneys were rapidly removed, frozen ST:STUDY_SUMMARY in liquid nitrogen and stored at -80C ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 19 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10114 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0012168 S00018110 Disease:GN | Treated:Treated SUBJECT_SAMPLE_FACTORS SU0012168 S00018111 Disease:GN | Treated:Treated SUBJECT_SAMPLE_FACTORS SU0012168 S00018112 Disease:GN | Treated:Treated SUBJECT_SAMPLE_FACTORS SU0012168 S00018113 Disease:GN | Treated:Vehicle SUBJECT_SAMPLE_FACTORS SU0012168 S00018114 Disease:GN | Treated:Vehicle SUBJECT_SAMPLE_FACTORS SU0012168 S00018115 Disease:GN | Treated:Vehicle SUBJECT_SAMPLE_FACTORS SU0012168 S00018116 Disease:GN | Treated:Treated SUBJECT_SAMPLE_FACTORS SU0012168 S00018117 Disease:GN | Treated:Treated SUBJECT_SAMPLE_FACTORS SU0012168 S00018118 Disease:GN | Treated:Treated SUBJECT_SAMPLE_FACTORS SU0012168 S00018119 Disease:GN | Treated:Treated SUBJECT_SAMPLE_FACTORS SU0012168 S00018120 Disease:GN | Treated:Treated SUBJECT_SAMPLE_FACTORS SU0012168 S00018121 Disease:GN | Treated:Treated SUBJECT_SAMPLE_FACTORS SU0012168 S00018122 Disease:GN | Treated:Vehicle SUBJECT_SAMPLE_FACTORS SU0012168 S00018123 Disease:GN | Treated:Vehicle SUBJECT_SAMPLE_FACTORS SU0012168 S00018124 Disease:GN | Treated:Vehicle SUBJECT_SAMPLE_FACTORS SU0012168 S00018125 Disease:Nl | Treated:Control SUBJECT_SAMPLE_FACTORS SU0012168 S00018126 Disease:Nl | Treated:Control SUBJECT_SAMPLE_FACTORS SU0012168 S00018127 Disease:Nl | Treated:Control #COLLECTION CO:COLLECTION_SUMMARY none CO:SAMPLE_TYPE Pooled sample #TREATMENT TR:TREATMENT_SUMMARY none #SAMPLEPREP SP:SAMPLEPREP_SUMMARY none SP:SAMPLEPREP_PROTOCOL_FILENAME 2014-10-07-Neurotransmitter.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE None CH:INSTRUMENT_NAME None CH:COLUMN_NAME Cogent Diamond Hydride CH:METHODS_FILENAME QM009_2mm_DH_Neurotransmitter_MRM-ESP-1.m.zip CH:METHODS_ID DQM0150 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6410A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg sample MS_METABOLITE_DATA_START S00018110 S00018111 S00018112 S00018113 S00018114 S00018115 S00018116 S00018117 S00018118 S00018119 S00018120 S00018121 S00018122 S00018123 S00018124 S00018125 S00018126 S00018127 5-Hydroxyindoleacetic acid 19.22 24.94 5.13 112.61 3.92 12.60 7.41 5.21 10.86 6.13 11.50 1.16 10.51 7.46 7.60 8.62 5.95 4.42 Adenosine 170.10 54.01 56.95 329.77 53.05 76.37 108.84 9.07 86.77 64.97 73.21 75.46 28.41 19.39 51.67 60.07 41.15 52.57 Serotonin 1.55 1.50 1.55 1.51 1.57 1.50 1.56 1.83 1.53 1.59 1.64 1.53 1.42 1.61 1.58 1.54 1.48 3-Methoxytyramine 0.76 0.76 0.76 0.76 0.76 0.75 0.76 0.76 0.75 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 Acetylcholine 3.33 0.19 0.18 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID 5-Hydroxyindoleacetic acid 1826 Adenosine 60961 Serotonin 5202 3-Methoxytyramine 1669 Acetylcholine 187 METABOLITES_END #END